CN103349165B - Chinese herbal medicine and biological bacterium compound feed additive, and preparation method and application of compound feed additive - Google Patents
Chinese herbal medicine and biological bacterium compound feed additive, and preparation method and application of compound feed additive Download PDFInfo
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- CN103349165B CN103349165B CN201310328340.2A CN201310328340A CN103349165B CN 103349165 B CN103349165 B CN 103349165B CN 201310328340 A CN201310328340 A CN 201310328340A CN 103349165 B CN103349165 B CN 103349165B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a Chinese herbal medicine and biological bacterium compound feed additive, and a preparation method and an application of the compound feed additive. The method comprises the steps that 5 parts of red ginseng, 5 parts of radix isatidis, 7 parts of folium isatidis, 5 parts of astragalus mongholicus, 10 parts of perillaseed, 25 parts of seatangle, 6 parts of herba leonuri, 5 parts of liquorice, 5 parts of tangerine peel and 10 parts of ganoderma applanatum are dried at a low temperature of 25-35 DEG C, crushed, sieved by a 60-mesh sieve, and mixed uniformly; an additive I is prepared; and then a microorganism fermentation additive II is prepared. The additive I and the additive II are combined for use; a prepared product can completely substitute an antibiotic; fed livestock (such as pigs) are free from drug residual ingredients through slaughtering detection; and the compound feed additive can achieve better disease prevention and resistance effects compared with the use of the antibiotic.
Description
Technical field
The present invention relates to field of feed processing, relate in particular to a kind of feed addictive and its production and use.
Background technology
At present, meat products industry be take fast-growing livestock and poultry and is supplied as main flow as meat products.The disadvantage of commercially available existence is antibiotic precipitation in a large number in the human body above, to human body, causes potential hazard to become common recognition, and the mouthfeel fishy smell of meat, flavour of a drug weight, and mouthfeel is poor.The generation that a small amount of raise pig scattered commercially available, although the pig that strolls etc. has reduced the problems referred to above to a certain extent; mouthfeel and the security of meat products have been increased to a certain extent; list still can not fully phase out antibiotic use; and this cultivating condition needs huge breeding spaces; to the right environment of ecology; destroy and be serious; if for example 10,000 square metres of ecological environments are put 20,000 pigs in a suitable place to breed; by not even a blade of grass grows; bark is all taken off light; be a kind of problem that in fact cannot solve large-scale cultivation, there is no sustainability and expansionary.
Summary of the invention
The object of the present invention is to provide a kind of Chinese herbal medicine and biological bacteria compound feed addictive.
Another object of the present invention is to provide the preparation method of Chinese herbal medicine and biological bacteria compound feed addictive.
Meanwhile, also provide a kind of feed and uses thereof.
Chinese herbal medicine provided by the present invention and biological bacteria compound feed addictive Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs,
5 parts of Radix Isatidis,
7 parts of folium isatidis,
5 parts of the Radixs Astragali,
10 parts of perillaseeds,
25 parts of kelps,
6 parts of motherworts,
5 parts, Radix Glycyrrhizae,
5 parts of dried orange peels,
10 parts of artist's conks,
Above-mentioned raw materials I being carried out under 25～40 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I;
Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain:
Described plastc ring is that the 2:4:3 ratio mixing by volume of saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension makes:
The preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 35～40 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
Preferably, described raw material I also comprises trace element, vitamin A vitamin D and amino acid 12-14 mass parts altogether.1-6 kind in described micro-chosen from Fe, copper, zinc, manganese, iodine, selenium.
Preferably, described raw material I also comprises the zeolite powder of 12-44 mass parts.
Preferably, described raw material I further comprises 7 parts of hawthorn, 5 parts, dandelion dry powder, 5 parts of Asiatic plantains.
The preparation method of Chinese herbal medicine and biological bacteria compound feed addictive, be to pulverize by 80 mesh sieves and mix finished product packing after raw material I is mixed by its weight proportion separately, during use, add in feed and mix simultaneously with biological bacteria fermentation raw material II again.
The claimed a kind of feed of the present invention, it contains above-mentioned Chinese herbal medicine and biological bacteria compound feed addictive.
Preferably, the additive I that described feed contains mass percent 1～3% and the additive II of mass percent 1%.
The purposes of this feed or feed addictive is the pig of feeding.
While not using, additive I and additive II bag distribution packaging
Technique effect of the present invention is:
1) this product substitute antibiotics completely, feed the domestic animal (for example pig) of raising and do not have medicament residue composition through butchering detection, and can reach than using the more excellent disease resistance effect of antibiotic, increase domestic animal autoimmunity, through test, the death rate is not more added this product contrast groups and has been reduced more than 85%, uses the antibiotic contrast groups incidence of disease to reduce by 15%, and the death rate has reduced by 8%; Reached and obviously significantly reduced to obtain effect; The interpolation of the additive described in this patent has improved the palatability of feed greatly, improves food conversion ratio, and reduces aquaculture cost;
2) butchering rear detection confirms: use feed addictive that this patent provides or production of fodder and pork be the green nourishing health type meat products such as a kind of low cholesterol, low fat, high protein, homoamino acid; Use the domestic animal of this forage feed, the whole collagen of meat is better abundant, various amino acid contents, apparently higher than common pork, are even better than new day oriental cherry pork (note: newly the rank of day oriental cherry pork in pork cultivation field is equivalent to Kobe beef in the rank in beef cultivation field); Refer to table 1, table 2:
Table 1 pork amino acid content contrast table
Amino acid content in every 100 grams of fresh porks
Table 2 fresh meat composition contrast table
Content in every 100 grams of fresh porks
| Title | Common pork | This product feed and pork |
| Fat | 88.6g | 15.4g |
| Cholesterol | 109mg | 29.2mg |
3) feed addictive or the Feed Energy that use this patent to provide carry out extensive industrialization cultivation, and mouthfeel and quality surpass the quality of common meat, refer to table 3; And it is minimum that breeding process forms destructiveness to ecological environment;
Table 3 this product is fed the mark sheets such as pork eating mouth feel of raising
* foregoing description is 30 conclusions that experienced evaluating member Comprehensive Assessment suggestion draws
The specific embodiment
Below by embodiment, further the present invention is set forth.
Embodiment 1
Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs, 5 parts of Radix Isatidis, 7 parts of folium isatidis, 5 parts of the Radixs Astragali, 10 parts of perillaseeds, 25 parts of kelps, 6 parts of motherworts, 5 parts, Radix Glycyrrhizae, 5 parts of dried orange peels, 10 parts of artist's conks;
Above-mentioned raw materials I being carried out under 25～35 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I; Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain:
Described plastc ring is that the 2:4:3 ratio mixing by volume of saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension makes: the preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 35～40 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
Addition in feed:
In piglet feed, add: the additive I of mass percent 1% and the additive II of mass percent 1%.
In pig starter feed, add: the additive I of mass percent 1.5% and the additive II of mass percent 1%.
In fattening pannage, add: the additive I of mass percent 2%～3% and the additive II of mass percent 1%.
Embodiment 2
Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs, 5 parts of Radix Isatidis, 7 parts of folium isatidis, 5 parts of the Radixs Astragali, 10 parts of perillaseeds, 25 parts of kelps, 6 parts of motherworts, 5 parts, Radix Glycyrrhizae, 5 parts of dried orange peels, 10 parts of artist's conks; 7 parts of hawthorn, 5 parts, dandelion dry powder, 5 parts of Asiatic plantains;
Above-mentioned raw materials I being carried out under 25～40 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I; Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain: described plastc ring be saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension by volume 2:4:3 ratio mix and make; The preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 25～35 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
Addition in feed:
In piglet feed, add: the additive I of mass percent 1% and the additive II of mass percent 1%.
In pig starter feed, add: the additive I of mass percent 1～1.5% and the additive II of mass percent 1%.
In fattening pannage, add: the additive I of mass percent 3% and the additive II of mass percent 1%.
Embodiment 3
Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs, 5 parts of Radix Isatidis, 7 parts of folium isatidis, 5 parts of the Radixs Astragali, 10 parts of perillaseeds, 25 parts of kelps, 6 parts of motherworts, 5 parts, Radix Glycyrrhizae, 5 parts of dried orange peels, 10 parts of artist's conks; Raw material I also comprises trace element, vitamin A vitamin D and amino acid 12-14 mass parts altogether; 1-6 kind in described micro-chosen from Fe, copper, zinc, manganese, iodine, selenium.Described raw material I also comprises the zeolite powder of 12-44 mass parts.
Above-mentioned raw materials I being carried out under 25～40 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I; Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain: described plastc ring be saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension by volume 2:4:3 ratio mix and make; The preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 35～40 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
Addition in feed:
In piglet feed, add: the additive I of mass percent 2% and the additive II of mass percent 1%.
In pig starter feed, add: the additive I of mass percent 2.5% and the additive II of mass percent 1%.
In fattening pannage, add: the additive I of mass percent 3% and the additive II of mass percent 1%.
Embodiment 4
Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs, 5 parts of Radix Isatidis, 7 parts of folium isatidis, 5 parts of the Radixs Astragali, 10 parts of perillaseeds, 25 parts of kelps, 6 parts of motherworts, 5 parts, Radix Glycyrrhizae, 5 parts of dried orange peels, 10 parts of artist's conks; 7 parts of hawthorn, 5 parts, dandelion dry powder, 5 parts of Asiatic plantains.Raw material I also comprises trace element, vitamin A vitamin D and amino acid totally 14 mass parts; 1-6 kind in described micro-chosen from Fe, copper, zinc, manganese, iodine, selenium.Described raw material I also comprises the zeolite powder of 44 mass parts.
Above-mentioned raw materials I being carried out under 25～35 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I; Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain; Described plastc ring is that the 2:4:3 ratio mixing by volume of saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension makes: the preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 35～40 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
Addition in feed:
In piglet feed, add: the additive I of mass percent 1% and the additive II of mass percent 1%.
In pig starter feed, add: the additive I of mass percent 1～1.5% and the additive II of mass percent 1%.
In fattening pannage, add: the additive I of mass percent 3% and the additive II of mass percent 1%.
Claims (10)
1. Chinese herbal medicine and biological bacteria compound feed addictive, be comprised of additive I and additive II, and its raw material comprises raw material I and microorganism fermentation raw material II:
Described above-mentioned raw materials I comprises following Chinese herbal medicine according to weight proportion:
5 parts of red ginsengs,
5 parts of Radix Isatidis,
7 parts of folium isatidis,
5 parts of the Radixs Astragali,
10 parts of perillaseeds,
25 parts of kelps,
6 parts of motherworts,
5 parts, Radix Glycyrrhizae,
5 parts of dried orange peels,
10 parts of artist's conks,
Above-mentioned raw materials I being carried out under 25～40 ℃ of conditions to low temperature drying pulverizes 80 orders and sieves to mix and make additive I;
Described microorganism fermentation raw material II comprises 20 parts, dregs of beans 30 50 parts, Fen ﹑ wheat bran and oily rice bran, adding quality is that the plastc ring of 0.8～1.2 times of dregs of beans, wheat bran and oily rice bran gross weight mixes again, and it is carried out under 25～35 ℃ of conditions to low temperature drying and pulverizes the 60 orders additive II of sieving to obtain:
Described plastc ring is that the 2:4:3 ratio mixing by volume of saccharomyces cerevisiae bacteria suspension, bafillus natto bacteria suspension and Lactobacillus plantarum bacteria suspension makes;
The preparation of described saccharomyces cerevisiae bacteria suspension is:
1) dull and stereotyped cultivation activates: saccharomyces cerevisiae is inoculated in to the dull and stereotyped cultivation of PDA upper, under 25 ℃ of conditions, cultivates 20～36h, form single bacterium colony;
2) saccharomycete of step 1) activation culture is inoculated in PDA Liquid Culture, under 25～30 ℃, 200～240rpm condition, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described bafillus natto bacteria suspension is:
1) dull and stereotyped cultivation activates: bafillus natto is inoculated in to the dull and stereotyped cultivation of LB upper, under 35～40 ℃ of conditions, cultivates 14～30h, form single bacterium colony;
2) the bafillus natto bacterial classification of step 1) activation culture is inoculated in LB Liquid Culture, under 35～40 ℃, 180～240rpm condition, cultivates 8～16h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby;
The preparation of described Lactobacillus plantarum bacteria suspension is:
1) dull and stereotyped cultivation activates: Lactobacillus plantarum is inoculated in to the dull and stereotyped cultivation of MRS upper, under 30～40 ℃ of conditions, cultivates 24～36h, form single bacterium colony;
2) Lactobacillus plantarum of step 1) activation culture is inoculated in MRS Liquid Culture, under 30～40 ℃ of conditions, cultivates 12～24h, make it in the logarithm middle and later periods;
3) by step 2) bacteria liquid that obtains centrifugal collection thalline under 4 ℃, 4000rpm condition, with SPSS, be diluted to 1.0～2.0 * 10
11individual/m1 is standby.
2. Chinese herbal medicine as claimed in claim 1 and biological bacteria compound feed addictive, is characterized in that: described raw material I also comprises trace element, vitamin A vitamin D and amino acid 12-14 mass parts altogether.
3. Chinese herbal medicine as claimed in claim 2 and biological bacteria compound feed addictive, is characterized in that: the 1-6 kind in described micro-chosen from Fe, copper, zinc, manganese, iodine, selenium.
4. Chinese herbal medicine as claimed in claim 1 and biological bacteria compound feed addictive, is characterized in that: described raw material I also comprises the zeolite powder of 12-44 mass parts.
5. Chinese herbal medicine as claimed in claim 1 and biological bacteria compound feed addictive, is characterized in that: described raw material I further comprises 7 parts of hawthorn, 5 parts, dandelion dry powder, 5 parts of Asiatic plantains.
6. a Chinese herbal medicine as described in claim 1-5 and the preparation method of biological bacteria compound feed addictive, be to pulverize by 80 mesh sieves and mix finished product packing after raw material I is mixed by its weight proportion separately, during use, add in feed and mix simultaneously with microorganism fermentation raw material II again.
7. a feed, is characterized in that the Chinese herbal medicine and the biological bacteria compound feed addictive that contain one of claim 1-5.
8. feed as claimed in claim 7, the additive I that it is characterized in that containing mass percent 1～3% and the additive II of mass percent 1%.
9. the purposes of Chinese herbal medicine and biological bacteria compound feed addictive described in claim 1-5, is characterized in that the pig of feeding.
10. the purposes of feed described in claim 7-8, is characterized in that the pig of feeding.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310328340.2A CN103349165B (en) | 2013-07-31 | 2013-07-31 | Chinese herbal medicine and biological bacterium compound feed additive, and preparation method and application of compound feed additive |
Applications Claiming Priority (1)
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| CN108477398A (en) * | 2018-03-31 | 2018-09-04 | 潍坊友容实业有限公司 | A kind of cattle feed additive/premixed material and preparation method thereof |
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