CN106173326A - A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent and preparation method thereof - Google Patents
A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent and preparation method thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent and preparation method thereof, including Chinese herbal medicine and compound probiotic, by weight, Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 30 40 parts, 30 40 parts of Radix Rehmanniae Preparata, Vaccaria segetalis 30 40 parts, Medulla Tetrapanacis 30 40 parts, Radix Codonopsis 20 30 parts, Herba Leonuri 20 30 parts, Radix Isatidis 20 30 parts, Radix Rhodiolae 20 30 parts, Herba Taraxaci 10 18 parts, Radix Bupleuri 10 18 parts, Folium Isatidis 10 18 parts, Radix Gentianae 58 parts, Fructus Crataegi 58 parts, Rhizoma Acori Graminei 58 parts；Compound probiotic is made up of following strain: bacillus subtilis 5 10 parts, bacillus thuringiensis 35 parts, bacillus acidophilus 35 parts, cereuisiae fermentum 5 10 parts.The milch cow of present invention Chinese herbal medicine probiotic bacteria composite biological agent, can effectively treat and prevent bovine subclinical mastitis.
Description
Technical field
The present invention relates to animal and fowl fodder technical field, particularly relate to a kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent
And preparation method thereof.
Background technology
Bovine subclinical mastitis is a kind of serious disease common, complicated, that cause dairy heavy losses, the most sternly
Ghost image rings the utilization periods of milk cattle, galactopoiesis quantity and dairy food quality, and is harmful to food hygiene and human health.For a long time with
Coming, cowboying family uses to the mode of milch cow long-term feeding antibiotic mostly to prevent bovine subclinical mastitis.Antibiotic has and kills
The effect that going out antibacterial and suppresses pathogenic microorganism, the most this feeding patterns serves certain prevention milch cow in actual production
The effect of latent mammitis.But, these antibiotic can eat milk along with people and various milk product enters human body, this
The most indirectly result in the abuse of antibiotic, it is possible to cause multiple drug-induced disease, even can produce new " super fastbacteria ",
Thus the life and health of the mankind in this feeding patterns serious threat.
Summary of the invention
The invention aims to overcome the deficiencies in the prior art, it is provided that a kind of milch cow is compound raw with Chinese herbal medicine probiotic bacteria
Thing preparation, by the mutually coordinated effect of probiotic bacteria Yu Chinese herbal medicine, in the presence of Chinese herbal medicine, the growing power of probiotic bacteria increases
By force, probiotic bacteria has facilitation to the metabolism of Chinese herbal medicine, and probiotic bacteria can effectively supplement, regulate or maintain Tiny ecosystem in animal intestinal
Balance, improves cow feeding amount, can activate the immunocyte in systemic blood, promotes that body produces immunoglobulin, improves
Immunoglobulin G and M level in dairy cow milk, strengthen the immunity of milch cow；Herbal component has promoting the circulation of blood and stimulates the menstrual flow, and expedites the emergence of down
Breast, detumescence such as holds back at the effect, additionally it is possible to the inflammation such as secretion anti-microbial type material, effectively prevention and treatment mammitis of cow, by probiotic bacteria
Carry out compounding with Chinese herbal medicine and be capable of cooperative effect, be greatly enhanced the immunocompetence of milch cow, promote Lactation of Dairy Cow and improve milk
Water quality；Strengthen cure the disease performance and growth promotion performance, effectively treat and prevent bovine subclinical mastitis, and then replace antibiosis completely
The use of element.
To achieve these goals, the present invention adopts the following technical scheme that
A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent, including Chinese herbal medicine and compound probiotic, by weight,
Described Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 30-40 part, Radix Rehmanniae Preparata 30-40 part, Vaccaria segetalis 30-40 part, Medulla Tetrapanacis 30-40 part, party
Ginseng 20-30 part, Herba Leonuri 20-30 part, Radix Isatidis 20-30 part, Radix Rhodiolae 20-30 part, Herba Taraxaci 10-18 part, Radix Bupleuri 10-18
Part, Folium Isatidis 10-18 part, Radix Gentianae 5-8 part, Fructus Crataegi 5-8 part, Rhizoma Acori Graminei 5-8 part；
Described compound probiotic is made up of following strain: bacillus subtilis 5-10 part, bacillus thuringiensis 3-5 part,
Bacillus acidophilus's 3-5 part, cereuisiae fermentum 5-10 part.
The described milch cow preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, comprises the following steps:
(1) ratio takes each Chinese herbal medicine by weight, after being pulverized respectively by Chinese herbal medicine, sieves, mixing, soaks, steaming and decocting
Three times, the filtrate after steaming and decocting and medicinal residues are mixed, lyophilizing, prepare Chinese medicine lyophilized powder；
(2) bacillus subtilis, bacillus thuringiensis, bacillus acidophilus, cereuisiae fermentum are individually placed to culture medium
In cultivate, be then purified cultivation, respectively obtain bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, addicted to acid
Lactobacillus bacterium solution, cereuisiae fermentum bacterium solution；By bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, bacillus acidophilus bacterium
Liquid, cereuisiae fermentum bacterium solution mix, and obtain mixed bacteria liquid；
(3) by weight, Chinese medicine lyophilized powder 20-30 part is put into fermentation tank, be subsequently adding enzyme 5-7 part, low
Poly-galactose 10-15 part, Angel Yeast 5-10 part, add mixed bacteria liquid 5-10 part, airtight, fermentation；Fermentation takes anaerobism to send out
The mode of ferment, the continuous fermentation time is 30-40 hour, and PH is 3.8-4.0.
Described in step (2), the preparation method of bacillus subtilis bacterium solution is: weighs bacillus subtilis sterilized water and joins
Make the bacterium solution that mass volume ratio is 3-5%, then measure bacterium solution 0.1ml and be inoculated on LBG fluid medium in 37 DEG C of cultivations
24h, when spore rate reaches more than 75%, makes fermentation liquid be cooled to 20 DEG C, obtains bacillus subtilis bacterium solution.
Described in step (2), the preparation method of bacillus thuringiensis bacterium solution is: weigh bacillus thuringiensis with aseptic
Water is configured to the bacterium solution that mass volume ratio is 3～5%, then measures bacterium solution 0.1ml and is inoculated on TPY fluid medium in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus thuringiensis bacterium solution.
Described in step (2), the preparation method of bacillus acidophilus's bacterium solution is: weighs bacillus acidophilus's sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on DMRS fluid medium in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus acidophilus's bacterium solution.
Described in step (2), the preparation method of cereuisiae fermentum bacterium solution is: weighs cereuisiae fermentum sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on potato dextrose broth in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain cereuisiae fermentum bacterium solution.
At least one in amylase, cellulase, pectase, lysozyme chloride of enzyme described in step (3).
The pharmacological property of each medicine of heretofore described herbal component is as follows:
Thallus Laminariae (Thallus Eckloniae): YIN nourishing suppressing the hyperactive liver, press down gas eliminating stagnation, organic iodine content is high also can improve milk yield rich in the lactogenesis factor.
Radix Rehmanniae Preparata: there is the effect of YIN nourishing of enriching blood.
Vaccaria segetalis: containing multiple Saponin, has promoting blood circulation to restore menstrual flow, reducing swelling and alleviating pain, expedites the emergence of the function of stimulating milk secretion.
Medulla Tetrapanacis: sweet in the mouth, light, cold nature, there is clearing away heat and promoting diuresis, the effects such as stimulating milk secretion is sensible.
Radix Codonopsis: sweet in the mouth is flat, invigorating the spleen and replenishing QI and taste, and its active component Saponin can improve immunity of organisms.
Herba Leonuri: blood circulation promoting and blood stasis dispelling, containing multiple alkaloid and organic selenium, can improve the immunity of body.
Radix Isatidis: there is heat-clearing and toxic substances removing, removing heat from blood antiinflammatory, its active component is indigo, indirubin etc. have resisting pathogenic microbes,
Strengthen immunologic function.
Radix Rhodiolae: main active is flavone and rhodioside can not only improve immunity of organisms and make that body is anti-to be lacked
Oxygen.
Herba Taraxaci: the flat sweet in the mouth of property can heat-clearing and toxic substances removing, dispersing swelling and dissipating binds is containing composition and the Radix Scutellariae such as taraxasterol, choline, inulin
Common suppression mastitis harmful bacteria (staphylococcus aureus and micrococcus scarlatinae).
Radix Bupleuri: bitter in the mouth, cold nature, returns liver and gall warp, has soothing liver-QI stomach function regulating, the function of yang invigorating, and anti-bacteria and anti-virus also has immunity
Regulatory function.
Folium Isatidis: active component is indirubin, its effect antiviral and enhancing immunity.
Radix Gentianae: have digestion promoting function, its active component gentiopicrin has direct hepatoprotective effect.
Fructus Crataegi: promoting digestion and removing stagnation, circulation of qi promoting dissipating blood stasis, antidiarrheal dysentery.
Rhizoma Acori Graminei: there is removing dampness appetizing, eliminating phlegm of having one's ideas straightened out, effect of refreshment Fructus Alpiniae Oxyphyllae.
Chinese herbal medicine used by the present invention is the treatment and prevention mechanism of the growth characteristic according to milch cow, bovine subclinical mastitis
And the cultivation demand during milk cattle cultivating, use monarch, minister, the drug matching principle helping, make, choose respectively can lactogenic and
Improve milk water quality, can strengthen milch cow immunity of organisms, can improve milch cow resistance against diseases, promote milch cow digestion and growth
A few herbs are formulated: first selecting Thallus Laminariae (Thallus Eckloniae), Radix Rehmanniae Preparata, Vaccaria segetalis, Medulla Tetrapanacis is monarch drug, can be with blood circulation promoting and blood stasis dispelling, lactogenesis and raising
Milk yield；Select Radix Codonopsis, Herba Leonuri, Radix Isatidis, Radix Rhodiolae to improve milch cow immunity of organisms, reach the purpose of benefiting qi and nourishing blood；With
Shi Xuanyong Herba Taraxaci, Radix Bupleuri, Folium Isatidis improve milch cow anti-bacteria and anti-virus ability；Radix Gentianae, Fructus Crataegi, Rhizoma Acori Graminei is finally selected to improve
Milch cow digestion power.As can be seen here, herbal component provided by the present invention can not only promote the speed of growth of milch cow, and
The disease resistance ability of milch cow can be strengthened, thus can effectively treat and prevent bovine subclinical mastitis.
Compared with the prior art, beneficial effects of the present invention is as follows:
The milch cow of present invention Chinese herbal medicine probiotic bacteria composite biological agent, by the mutually coordinated work of probiotic bacteria Yu Chinese herbal medicine
With, in the presence of Chinese herbal medicine, the growing power of probiotic bacteria strengthens, and probiotic bacteria has facilitation to the metabolism of Chinese herbal medicine, benefit
Microecological balance in animal intestinal can effectively be supplemented, regulate or be maintained to raw bacterium, improves cow feeding amount, can activate systemic blood
In immunocyte, promote that body produces immunoglobulin, improve immunoglobulin G and M level in dairy cow milk, strengthen milch cow
Immunity；Herbal component has promoting the circulation of blood and stimulates the menstrual flow, and expedites the emergence of stimulating milk secretion, and detumescence such as holds back at the effect, additionally it is possible to secretion anti-microbial type material, has
Effect prevention and the treatment inflammation such as mammitis of cow, carry out probiotic bacteria and Chinese herbal medicine compounding being capable of cooperative effect, increase greatly
The immunocompetence of strong milch cow, promotes Lactation of Dairy Cow and improves milk water quality；Strengthen cure the disease performance and growth promotion performance, effectively treat
With prevention bovine subclinical mastitis, and then replace the use of antibiotic completely.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but the present invention is not limited only to these embodiments, not
On the premise of departing from present inventive concept, within any improvement made all falls within protection scope of the present invention.
Embodiment 1:
A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent, including Chinese herbal medicine and compound probiotic, by weight,
Described Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 30 parts, 40 parts of Radix Rehmanniae Preparata, Vaccaria segetalis 30 parts, Medulla Tetrapanacis 40 parts, Radix Codonopsis 20 parts, Herba Leonuri
Grass 20 parts, Radix Isatidis 30 parts, Radix Rhodiolae 30 parts, Herba Taraxaci 10 parts, Radix Bupleuri 10 parts, Folium Isatidis 18 parts, Radix Gentianae 5 parts, Fructus Crataegi 5
Part, Rhizoma Acori Graminei 8 parts；
Described compound probiotic is made up of following strain: bacillus subtilis 5 parts, bacillus thuringiensis 3 parts, addicted to yogurt
Bacillus 5 parts, cereuisiae fermentum 10 parts.
The described milch cow preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, comprises the following steps:
(1) ratio takes each Chinese herbal medicine by weight, after being pulverized respectively by Chinese herbal medicine, sieves, mixing, soaks, steaming and decocting
Three times, the filtrate after steaming and decocting and medicinal residues are mixed, lyophilizing, prepare Chinese medicine lyophilized powder；
(2) bacillus subtilis, bacillus thuringiensis, bacillus acidophilus, cereuisiae fermentum are individually placed to culture medium
In cultivate, be then purified cultivation, respectively obtain bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, addicted to acid
Lactobacillus bacterium solution, cereuisiae fermentum bacterium solution；By bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, bacillus acidophilus bacterium
Liquid, cereuisiae fermentum bacterium solution mix, and obtain mixed bacteria liquid；
(3) by weight, Chinese medicine lyophilized powder 20 parts is put into fermentation tank, be subsequently adding enzyme 5 parts, oligomeric gala
Sugar 10 parts, Angel Yeast 5 parts, add mixed bacteria liquid 10 parts, airtight, fermentation；The mode of anaerobic fermentation is taked in fermentation, sends out continuously
The ferment time is 30 hours, and PH is 4.0.
Described in step (2), the preparation method of bacillus subtilis bacterium solution is: weighs bacillus subtilis sterilized water and joins
Make the bacterium solution that mass volume ratio is 3-5%, then measure bacterium solution 0.1ml and be inoculated on LBG fluid medium in 37 DEG C of cultivations
24h, when spore rate reaches more than 75%, makes fermentation liquid be cooled to 20 DEG C, obtains bacillus subtilis bacterium solution.
Described in step (2), the preparation method of bacillus thuringiensis bacterium solution is: weigh bacillus thuringiensis with aseptic
Water is configured to the bacterium solution that mass volume ratio is 3～5%, then measures bacterium solution 0.1ml and is inoculated on TPY fluid medium in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus thuringiensis bacterium solution.
Described in step (2), the preparation method of bacillus acidophilus's bacterium solution is: weighs bacillus acidophilus's sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on DMRS fluid medium in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus acidophilus's bacterium solution.
Described in step (2), the preparation method of cereuisiae fermentum bacterium solution is: weighs cereuisiae fermentum sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on potato dextrose broth in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain cereuisiae fermentum bacterium solution.
Enzyme described in step (3) is selected from amylase.
Embodiment 2:
A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent, including Chinese herbal medicine and compound probiotic, by weight,
Described Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 35 parts, 35 parts of Radix Rehmanniae Preparata, Vaccaria segetalis 35 parts, Medulla Tetrapanacis 35 parts, Radix Codonopsis 25 parts, Herba Leonuri
Grass 25 parts, Radix Isatidis 25 parts, Radix Rhodiolae 25 parts, Herba Taraxaci 15 parts, Radix Bupleuri 15 parts, Folium Isatidis 15 parts, Radix Gentianae 6 parts, Fructus Crataegi 6
Part, Rhizoma Acori Graminei 6 parts；
Described compound probiotic is made up of following strain: bacillus subtilis 7 parts, bacillus thuringiensis 4 parts, addicted to yogurt
Bacillus 4 parts, cereuisiae fermentum 7 parts.
The described milch cow preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, comprises the following steps:
(1) ratio takes each Chinese herbal medicine by weight, after being pulverized respectively by Chinese herbal medicine, sieves, mixing, soaks, steaming and decocting
Three times, the filtrate after steaming and decocting and medicinal residues are mixed, lyophilizing, prepare Chinese medicine lyophilized powder；
(2) bacillus subtilis, bacillus thuringiensis, bacillus acidophilus, cereuisiae fermentum are individually placed to culture medium
In cultivate, be then purified cultivation, respectively obtain bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, addicted to acid
Lactobacillus bacterium solution, cereuisiae fermentum bacterium solution；By bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, bacillus acidophilus bacterium
Liquid, cereuisiae fermentum bacterium solution mix, and obtain mixed bacteria liquid；
(3) by weight, Chinese medicine lyophilized powder 25 parts is put into fermentation tank, be subsequently adding enzyme 6 parts, oligomeric gala
Sugar 12 parts, Angel Yeast 7 parts, add mixed bacteria liquid 7 parts, airtight, fermentation；The mode of anaerobic fermentation is taked in fermentation, sends out continuously
The ferment time is 35 hours, and PH is 3.8.
Described in step (2), the preparation method of bacillus subtilis bacterium solution is: weighs bacillus subtilis sterilized water and joins
Make the bacterium solution that mass volume ratio is 3-5%, then measure bacterium solution 0.1ml and be inoculated on LBG fluid medium in 37 DEG C of cultivations
24h, when spore rate reaches more than 75%, makes fermentation liquid be cooled to 20 DEG C, obtains bacillus subtilis bacterium solution.
Described in step (2), the preparation method of bacillus thuringiensis bacterium solution is: weigh bacillus thuringiensis with aseptic
Water is configured to the bacterium solution that mass volume ratio is 3～5%, then measures bacterium solution 0.1ml and is inoculated on TPY fluid medium in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus thuringiensis bacterium solution.
Described in step (2), the preparation method of bacillus acidophilus's bacterium solution is: weighs bacillus acidophilus's sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on DMRS fluid medium in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus acidophilus's bacterium solution.
Described in step (2), the preparation method of cereuisiae fermentum bacterium solution is: weighs cereuisiae fermentum sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on potato dextrose broth in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain cereuisiae fermentum bacterium solution.
Enzyme described in step (3) is selected from amylase, cellulase.
Embodiment 3:
A kind of milch cow Chinese herbal medicine probiotic bacteria composite biological agent, including Chinese herbal medicine and compound probiotic, by weight,
Described Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 40 parts, 40 parts of Radix Rehmanniae Preparata, Vaccaria segetalis 30 parts, Medulla Tetrapanacis 30 parts, Radix Codonopsis 30 parts, Herba Leonuri
Grass 30 parts, Radix Isatidis 20 parts, Radix Rhodiolae 200 parts, Herba Taraxaci 18 parts, Radix Bupleuri 18 parts, Folium Isatidis 10 parts, Radix Gentianae 8 parts, Fructus Crataegi 8
Part, Rhizoma Acori Graminei 5 parts；
Described compound probiotic is made up of following strain: bacillus subtilis 10 parts, bacillus thuringiensis 5 parts, addicted to acid
Lactobacillus 5 parts, cereuisiae fermentum 5 parts.
The described milch cow preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, comprises the following steps:
(1) ratio takes each Chinese herbal medicine by weight, after being pulverized respectively by Chinese herbal medicine, sieves, mixing, soaks, steaming and decocting
Three times, the filtrate after steaming and decocting and medicinal residues are mixed, lyophilizing, prepare Chinese medicine lyophilized powder；
(2) bacillus subtilis, bacillus thuringiensis, bacillus acidophilus, cereuisiae fermentum are individually placed to culture medium
In cultivate, be then purified cultivation, respectively obtain bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, addicted to acid
Lactobacillus bacterium solution, cereuisiae fermentum bacterium solution；By bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, bacillus acidophilus bacterium
Liquid, cereuisiae fermentum bacterium solution mix, and obtain mixed bacteria liquid；
(3) by weight, Chinese medicine lyophilized powder 30 parts is put into fermentation tank, be subsequently adding enzyme 7 parts, oligomeric gala
Sugar 15 parts, Angel Yeast 10 parts, add mixed bacteria liquid 10 parts, airtight, fermentation；The mode of anaerobic fermentation is taked in fermentation, continuously
Fermentation time is 40 hours, and PH is 4.0.
Described in step (2), the preparation method of bacillus subtilis bacterium solution is: weighs bacillus subtilis sterilized water and joins
Make the bacterium solution that mass volume ratio is 3-5%, then measure bacterium solution 0.1ml and be inoculated on LBG fluid medium in 37 DEG C of cultivations
24h, when spore rate reaches more than 75%, makes fermentation liquid be cooled to 20 DEG C, obtains bacillus subtilis bacterium solution.
Described in step (2), the preparation method of bacillus thuringiensis bacterium solution is: weigh bacillus thuringiensis with aseptic
Water is configured to the bacterium solution that mass volume ratio is 3～5%, then measures bacterium solution 0.1ml and is inoculated on TPY fluid medium in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus thuringiensis bacterium solution.
Described in step (2), the preparation method of bacillus acidophilus's bacterium solution is: weighs bacillus acidophilus's sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on DMRS fluid medium in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus acidophilus's bacterium solution.
Described in step (2), the preparation method of cereuisiae fermentum bacterium solution is: weighs cereuisiae fermentum sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on potato dextrose broth in 37 DEG C
Cultivate 24h, when spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain cereuisiae fermentum bacterium solution.
Enzyme described in step (3) is selected from pectase, lysozyme chloride.
Test example:
(1) toxicity test
Select SD rat, give the biological preparation of the present invention, after molten water every day gavage once, continuous 90 days.During test
Observe the outward appearance of animal, general behavior, food ration, body weight change, be administered latter 90 days and drug withdrawal within 2 weeks, carry out hematology (RBC, HB,
Reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB,
CHOL), the index checking such as urine biochemistry, organ coefficient, histopathology.Result of the test shows: the preparation of the present invention feeds
Rat is in good condition, and outward appearance sign, behavioral activity, food-intake and body weight increase change the most without exception；Hematological examination, blood life
Chemistry, urine biochemical analysis all between normal range, group without significant difference；Each group main organs histopathological examination has no bright
Aobvious abnormal.These parameters drug withdrawal also had no change after 2 weeks.Preparation according to the result of the test present invention is administered rat for continuous 90 days
Having no significant effect, without clear and definite toxicity target organ and sensitive indicator, convalescent period observes and also has no retardance toxic reaction, and prompting is originally
The dose safety of the pharmaceutical preparation clinical practice of invention is higher.
(2) therapeutic test
1, the therapeutic outcome to milch cow clinic mastitis
(1) test method:
Experimental animal is selected from the ill milch cow of clinic mastitis of certain cattle farm, and symptom is that the ill district of mammary gland is red, swollen, surface
Temperature raises, the sensitivity touched, and volume increases, and lactation amount substantially reduces or stops, milk abnormal quality, in pale yellow or light red,
Milk is thin or thickness, or floccule occurs.Select Niu Zuowei this test of cure object that this type of symptom occurs, but with seriously
Systemic infection symptom, super acute or recurrence sick cattle except.Selecting the 50 example disease cattle meeting above-mentioned standard, stochastic averagina is divided into
5 groups, often organize 10 example disease cattle.The 1st group of preparation using embodiment 1, feeds 1 every day；The 2nd group of preparation using embodiment 2, often
It feeds 1 time；The 3rd group of preparation using embodiment 3, feeds 1 every day；4th group uses antibiotic, every day 1 time；5th group is not
Medication compares, and does not make any treatment and processes.
(2) criterion of therapeutical effect:
Recovery from illness: breast is red, swollen, hot, bitterly, scleroma disappear, milk perusal milk is uniform, without luming, milk yield rise；
Appetite, body temperature, spirit etc. all recover normal.
Effective: breast red and swollen heat pain is clearly better, and lump in breast substantially reduces, heating paresthesia disappears, and milk character is basic
Normally, milk is without grumeleuse or a small amount of milk block, and milk yield gos up but is not reaching to premorbid level.
Invalid: breast is red, swollen, hot, bitterly, scleroma be not improved or more serious, milk is abnormal, and milk yield declines；Controlling
During treatment, the course of disease does not subtracts.
(3) according to clinical symptoms and the curative effect changing comprehensive descision medicine of milk quality, and each group of mastitis milk is calculated
Cattle healing number, significant figure and invalid number in 50 days, is shown in Table 1:
The effect of table 1 invention formulation treatment clinical type mammitis of cow
| Group | Sick cattle number (example) | Cure number (example) | Significant figure (example) | Invalid number (example) | Cure rate (%) |
| 1 | 10 | 7 | 4 | 0 | 70% |
| 2 | 10 | 9 | 1 | 0 | 90% |
| 3 | 10 | 8 | 3 | 0 | 80% |
| 4 | 10 | 3 | 3 | 4 | 30% |
| 5 | 10 | 0 | 0 | 15 | 0 |
As shown in Table 1, invention formulation reaches 90% to the cure rate of clinical type mammitis of cow, wherein embodiment 2
Therapeutic effect is best.
2. the impact on milk production of cow
Observing the milk yield situation of above-mentioned 5 groups of cattle, result is as shown in table 2.
The impact (unit: kg/ group) on milk production of cow of table 2 invention formulation
Note: before medication, milk yield is the milk yield that medication is surveyed the previous day, refers to after medicine start meter after first time medication
Number
* pole significant difference, P ＜ 0.05 are represented；
Showing from table 2 result, the milk production of cow that invention formulation feeds substantially increases, and the preparation effect of embodiment 2 is
Good.
Claims (7)
1. a milch cow Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that: include Chinese herbal medicine and compound probiotic, press
Weight portion meter, described Chinese herbal medicine is composed of the following components: Thallus Laminariae (Thallus Eckloniae) 30-40 part, Radix Rehmanniae Preparata 30-40 part, Vaccaria segetalis 30-40 part, Medulla Tetrapanacis
30-40 part, Radix Codonopsis 20-30 part, Herba Leonuri 20-30 part, Radix Isatidis 20-30 part, Radix Rhodiolae 20-30 part, Herba Taraxaci 10-18 part,
Radix Bupleuri 10-18 part, Folium Isatidis 10-18 part, Radix Gentianae 5-8 part, Fructus Crataegi 5-8 part, Rhizoma Acori Graminei 5-8 part；
Described compound probiotic is made up of following strain: bacillus subtilis 5-10 part, bacillus thuringiensis 3-5 part, addicted to acid
Lactobacillus 3-5 part, cereuisiae fermentum 5-10 part.
2. a milch cow as claimed in claim 1 preparation method for Chinese herbal medicine probiotic bacteria composite biological agent, its feature exists
In: comprise the following steps:
(1) ratio takes each Chinese herbal medicine by weight, after being pulverized respectively by Chinese herbal medicine, sieves, mixing, soaks, steaming and decocting three times,
Filtrate after steaming and decocting and medicinal residues are mixed, lyophilizing, prepare Chinese medicine lyophilized powder；
(2) it is individually placed in culture medium enter by bacillus subtilis, bacillus thuringiensis, bacillus acidophilus, cereuisiae fermentum
Row cultivate, be then purified cultivation, respectively obtain bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, addicted to yogurt bar
Bacterium bacterium solution, cereuisiae fermentum bacterium solution；By bacillus subtilis bacterium solution, bacillus thuringiensis bacterium solution, bacillus acidophilus's bacterium solution, beer
Brewer yeast bacterium bacterium solution mixes, and obtains mixed bacteria liquid；
(3) by weight, Chinese medicine lyophilized powder 20-30 part is put into fermentation tank, be subsequently adding enzyme 5-7 part, oligomeric half
Lactose 10-15 part, Angel Yeast 5-10 part, add mixed bacteria liquid 5-10 part, airtight, fermentation；Anaerobic fermentation is taked in fermentation
Mode, the continuous fermentation time is 30-40 hour, and PH is 3.8-4.0.
The milch cow the most according to claim 2 preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that
Described in step (2), the preparation method of bacillus subtilis bacterium solution is: weighs bacillus subtilis sterilized water and is configured to quality
Volume ratio is the bacterium solution of 3-5%, then measures bacterium solution 0.1ml and is inoculated on LBG fluid medium in 37 DEG C of cultivation 24h, spore
When rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus subtilis bacterium solution.
The milch cow the most according to claim 2 preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that:
Described in step (2), the preparation method of bacillus thuringiensis bacterium solution is: weighs bacillus thuringiensis sterilized water and is configured to
Mass volume ratio is the bacterium solution of 3～5%, then measures bacterium solution 0.1ml and is inoculated on TPY fluid medium in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus thuringiensis bacterium solution.
The milch cow the most according to claim 2 preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that:
Described in step (2), the preparation method of bacillus acidophilus's bacterium solution is: weighs bacillus acidophilus's sterilized water and is configured to quality volume
Than the bacterium solution for 3-5%, then measuring bacterium solution 0.1ml and be inoculated on DMRS fluid medium in 37 DEG C of cultivation 24h, spore rate reaches
During to more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain bacillus acidophilus's bacterium solution.
The milch cow the most according to claim 2 preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that:
Described in step (2), the preparation method of cereuisiae fermentum bacterium solution is: weighs cereuisiae fermentum sterilized water and is configured to quality volume
Than the bacterium solution for 3-5%, then measure bacterium solution 0.1ml and be inoculated on potato dextrose broth in 37 DEG C of cultivation 24h,
When spore rate reaches more than 75%, make fermentation liquid be cooled to 20 DEG C, obtain cereuisiae fermentum bacterium solution.
The milch cow the most according to claim 2 preparation method of Chinese herbal medicine probiotic bacteria composite biological agent, it is characterised in that
At least one in amylase, cellulase, pectase, lysozyme chloride of enzyme described in step (3).
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| CN108185167A (en) * | 2018-02-28 | 2018-06-22 | 普安县凯升农业发展有限公司 | A kind of nursing period white feed for goat and preparation method thereof |
| CN108785415A (en) * | 2018-08-23 | 2018-11-13 | 普定县科技服务中心 | A kind of drug and preparation method thereof for treating sow mammitis |
| CN111066970A (en) * | 2019-12-30 | 2020-04-28 | 湖南百宜饲料科技有限公司 | Feed additive for improving reproductive performance of ternary sows |
| CN113439798A (en) * | 2021-05-28 | 2021-09-28 | 山西农业大学 | Enzyme and microecological preparation compound agent and preparation method thereof |
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| CN103349165A (en) * | 2013-07-31 | 2013-10-16 | 刘范涛 | Chinese herbal medicine and biological bacterium compound feed additive, and preparation method and application of compound feed additive |
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| CN1853497A (en) * | 2005-04-20 | 2006-11-01 | 上海泓宝绿色水产科技发展有限公司 | Improvement of cow milk output and milk quality by Chinese medicinal herb composite microbial forage additive |
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
| CN102366023A (en) * | 2011-09-16 | 2012-03-07 | 汪松林 | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics |
| CN103349165A (en) * | 2013-07-31 | 2013-10-16 | 刘范涛 | Chinese herbal medicine and biological bacterium compound feed additive, and preparation method and application of compound feed additive |
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| CN107865218A (en) * | 2017-11-29 | 2018-04-03 | 衡阳香樟苑生态农业发展科技有限公司 | A kind of milk cow high-yield feed and preparation method thereof |
| CN108185167A (en) * | 2018-02-28 | 2018-06-22 | 普安县凯升农业发展有限公司 | A kind of nursing period white feed for goat and preparation method thereof |
| CN108785415A (en) * | 2018-08-23 | 2018-11-13 | 普定县科技服务中心 | A kind of drug and preparation method thereof for treating sow mammitis |
| CN111066970A (en) * | 2019-12-30 | 2020-04-28 | 湖南百宜饲料科技有限公司 | Feed additive for improving reproductive performance of ternary sows |
| CN113439798A (en) * | 2021-05-28 | 2021-09-28 | 山西农业大学 | Enzyme and microecological preparation compound agent and preparation method thereof |
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Application publication date: 20161207 |