CN104491203A - Chinese herbal medicine fermentative extract liquid for preventing and treating cow mastitis and preparation method of Chinese herbal medicine fermentative extract liquid - Google Patents
Chinese herbal medicine fermentative extract liquid for preventing and treating cow mastitis and preparation method of Chinese herbal medicine fermentative extract liquid Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/86—Violaceae (Violet family)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
The invention discloses a Chinese herbal medicine fermentative extract liquid for preventing and treating cow mastitis and a preparation method of the Chinese herbal medicine fermentative extract liquid. The Chinese herbal medicine fermentative extract liquid is prepared from the following raw materials in parts by weight: 10-15 parts of dandelion, 5-15 parts of viola philippica, 5-10 parts of honeysuckle, 5-12 parts of ligusticum wallichii, 5-10 parts of angelica sinensis, 1-10 parts of pericarpium citri reticulatae viride, 10-20 parts of cowherb seed, 5-10 parts of ricepaperplant pith, 1-3 parts of liquorice and 1-3 parts of lactobacillus acidophilus. The Chinese herbal medicine fermentative extract liquid disclosed by the invention is applied to cow breeding, so that the Chinese herbal medicine fermentative extract liquid can play a protecting role on the cow mastitis; the non-specific immune function of the organism can be improved; the milk yield is improved; the quality of the milk is improved; relatively large economic benefits are obtained; and the farming enthusiasm of a farmer is improved.
Description
Technical field
The present invention relates to a kind of veterinary drug, be specifically related to a kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow and preparation method thereof.
Background technology
Mammitis of cow is one of principal disease of harm milk cow production, the cause of disease of this disease can be caused much to comprise: environmental management factor, pathogenic infection, cattle oneself factor, secondary factors.Milk cattle infected this after being ill, showing as breast has the inflammatory phenomena such as red, swollen, hot, pain, and trouble bovine lactation function and milk yield can be made sharply to decline and even stop, causing milch cow Reproductive Performance to lack of proper care, milk also changes thereupon.
Control mammitis of cow mainly uses antibiotic, hormone and chemical synthetic drug, and due to prolonged and repeated use related drugs, the drug resistance of antibacterial is more and more serious, reduces curative effect of medication.Along with people require more and more higher to quality of life, the requirement of people to livestock product safety and quality is also more and more higher.But life-time service chemicals, easily cause Lac Bovis seu Bubali drug residue, affect the quality of milk product, the health of harm humans.
Summary of the invention
The object of the invention is the defect for prior art, a kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow is provided, a kind of preparation method of preventing and treating the Chinese herbal medicine fermented extracted liquid of mammitis of cow is also provided.
In order to realize above object, the technical solution adopted in the present invention is:
Prevent and treat a Chinese herbal medicine fermented extracted liquid for mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 10 parts ~ 15 parts, Herba Violae 5 parts ~ 15 parts, Flos Lonicerae 5 parts ~ 10 parts, Rhizoma Chuanxiong 5 parts ~ 12 parts, Radix Angelicae Sinensis 5 parts ~ 10 parts, 1 part ~ 10 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 10 parts ~ 20 parts, Medulla Tetrapanacis 5 parts ~ 10 parts, 1 ~ 3 part, Radix Glycyrrhizae, bacillus acidophilus 1 ~ 3 part.
Prevent and treat a Chinese herbal medicine fermented extracted liquid for mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 15 parts, Herba Violae 10 parts, Flos Lonicerae 8 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 8 parts, 6 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 18 parts, Medulla Tetrapanacis 7 parts, 1 part, Radix Glycyrrhizae, bacillus acidophilus 2 parts.
Prevent and treat a preparation method for the Chinese herbal medicine fermented extracted liquid of mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci, Herba Violae, Flos Lonicerae, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Pericarpium Citri Reticulatae Viride, Semen Vaccariae, Medulla Tetrapanacis, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 5 ~ 6 times of water, decoct 1 ~ 2 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation; In cultured slant strains, add the sterilized normal saline of 1 ~ 3ml, prepare bacteria suspension; By bacteria suspension anaerobism amplification culture in the culture medium of correspondence that slant culture produces, be 1.0 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 1 ~ 3 part in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and subpackage, obtains Chinese herbal medicine fermented extracted liquid.
The culture medium of carrying out anaerobism amplification culture described in step (2) is MRS culture medium.
Described in step (3), the temperature of anaerobic fermentation is 30 ~ 37 DEG C, and fermentation time is 24 ~ 48 hours.
The present invention selects effect of raw material to be summarized as follows:
Herba Taraxaci: heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome.Cure mainly furuncle swelling toxin, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, jaundice due to damp-heat, the puckery pain of pyretic stranguria.Modern medical theory proves that Herba Taraxaci has stronger bactericidal action.
Herba Violae: heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling.Cure mainly furuncle swelling toxin, carbuncle carbuncle on the back, erysipelas, venom.
Flos Lonicerae: heat-clearing and toxic substances removing, wind-heat dissipating.Cure mainly carbuncle furuncle, sore throat, erysipelas, warm blood fulminant dysentery, anemopyretic cold, pestilence is generated heat.Modern medical theory proves that Flos Lonicerae has antibacterial, antiviral; Antipyretic; The effects such as antiinflammatory.
Rhizoma Chuanxiong: blood-activating and qi-promoting, wind-expelling pain-stopping.
Radix Angelicae Sinensis: enrich blood; Invigorate blood circulation; Menstruction regulating and pain relieving; Moisturize laxation.
Pericarpium Citri Reticulatae Viride: soothing the liver dispelling the stagnated QI, removing food stagnancyization is stagnant.Cure mainly sternal rib pain, hernia, nodule in the breast, acute mastitis, abdominal pain due to retention of food.
Semen Vaccariae: lactogenic, stimulates the menstrual flow, detumescence, pain relieving.There is promoting blood circulation to restore menstrual flow, reducing swelling and alleviating pain, expedite the emergence of the function of stimulating milk secretion.
Medulla Tetrapanacis: clearing away heat and promoting diuresis, ventilation stimulating milk secretion.Cure mainly hygropyrexia dark coloured urine, the puckery pain of gonorrhea, edema oliguria, agalactia.
Radix Glycyrrhizae: invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
Chinese herbal medicine treatment mammitis of cow in have immeasurable effect, present medical research shows that the active ingredient of many Chinese herbal medicine has the effects such as anti-inflammation, drug effect and antibiotic medicine very nearly the same.Chinese herbal medicine contains multiple nutritional components and active substance, has the double effects of medicine and nutrition, both can be improved the immunologic function of body by bactericidal action, treatment organism disease, and the utilization rate that can improve again feedstuff increases milk amount, improves milk quality.Therefore adopt Chinese herbal treatment mammitis of cow not only to ensure that the health of milch cow, ensure that the safety of milk simultaneously.
In recent years probiotic bacteria being applied to aquaculture is a large study hotspot.It has ill curing the disease, not sick diseases prevention, effect of anosis health care.Changed into the micromolecule being easy to body and absorbing by the active ingredient of the Chinese herbal medicine after viable bacteria fermentation by macromole, served that curative effect is fast, the effect of definite effect.Viable bacteria itself also has adjustment flora, suppresses pathogenic microorganism, strengthens the effect of resistance against diseases.The two plays a multiplier role.Therefore the development of Chinese herbal medicine and viable bacteria Chinese herbal medicine fermented extracted liquid by fermentation, can be described as the Gospel of numerous raisers, create larger economic benefit.
The Chinese herbal medicine fermented extracted liquid of the control mammitis of cow adopting technique scheme to obtain has the following advantages: improve immunity of organisms, prevention and therapy bovine mastitis; Supplement the nutrients, enhancing substance metabolism, improves the quality and quantity of milk; Milk Chinese medicine zero remains.
Detailed description of the invention
Embodiment 1:
Prevent and treat a Chinese herbal medicine fermented extracted liquid for mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 10 parts ~ 15 parts, Herba Violae 5 parts ~ 15 parts, Flos Lonicerae 5 parts ~ 10 parts, Rhizoma Chuanxiong 5 parts ~ 12 parts, Radix Angelicae Sinensis 5 parts ~ 10 parts, 1 part ~ 10 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 10 parts ~ 20 parts, Medulla Tetrapanacis 5 parts ~ 10 parts, 1 ~ 3 part, Radix Glycyrrhizae, bacillus acidophilus 1 ~ 3 part.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci, Herba Violae, Flos Lonicerae, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Pericarpium Citri Reticulatae Viride, Semen Vaccariae, Medulla Tetrapanacis, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 5 ~ 6 times of water, decoct 1 ~ 2 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 1.0 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 1 ~ 3 part in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 30 ~ 37 DEG C, and fermentation time is 24 ~ 48 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
Embodiment 2:
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 15 parts, Herba Violae 10 parts, Flos Lonicerae 8 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 8 parts, 6 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 18 parts, Medulla Tetrapanacis 7 parts, 1 part, Radix Glycyrrhizae, bacillus acidophilus 2 parts.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci 15 parts, Herba Violae 10 parts, Flos Lonicerae 8 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 8 parts, 6 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 18 parts, Medulla Tetrapanacis 7 parts, 1 part, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 5 times of water, decoct 1 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 1ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 1.0 × 10 to exponential phase clump count
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 2 parts in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 30 DEG C, and fermentation time is 24 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
Embodiment 3:
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 10 parts, Herba Violae 5 parts, Flos Lonicerae 5 parts, Rhizoma Chuanxiong 5 parts, Radix Angelicae Sinensis 5 parts, 1 part, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 10 parts, Medulla Tetrapanacis 5 parts, 1 part, Radix Glycyrrhizae, bacillus acidophilus 3 parts.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci 10 parts, Herba Violae 5 parts, Flos Lonicerae 5 parts, Rhizoma Chuanxiong 5 parts, Radix Angelicae Sinensis 5 parts, 1 part, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 10 parts, Medulla Tetrapanacis 5 parts, 1 part, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 6 times of water, decoct 1 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 3ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 1.0 × 10 to exponential phase clump count
8during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 3 parts in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 37 DEG C, and fermentation time is 24 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
Embodiment 4:
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 12 parts, Herba Violae 8 parts, Flos Lonicerae 6 parts, Rhizoma Chuanxiong 10 parts, Radix Angelicae Sinensis 7 parts, 7 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 15 parts, Medulla Tetrapanacis 10 parts, 2 parts, Radix Glycyrrhizae, bacillus acidophilus 1 part.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci 12 parts, Herba Violae 8 parts, Flos Lonicerae 6 parts, Rhizoma Chuanxiong 10 parts, Radix Angelicae Sinensis 7 parts, 7 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 15 parts, Medulla Tetrapanacis 10 parts, 2 parts, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 6 times of water, decoct 1 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 2ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 1.0 × 10 to exponential phase clump count
9during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 1 part in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 33 DEG C, and fermentation time is 24 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
Embodiment 5:
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 13 parts, Herba Violae 15 parts, Flos Lonicerae 10 parts, Rhizoma Chuanxiong 8 parts, Radix Angelicae Sinensis 10 parts, 10 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 20 parts, Medulla Tetrapanacis 8 parts, 3 parts, Radix Glycyrrhizae, bacillus acidophilus 2 parts.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci 13 parts, Herba Violae 15 parts, Flos Lonicerae 10 parts, Rhizoma Chuanxiong 8 parts, Radix Angelicae Sinensis 10 parts, 10 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 20 parts, Medulla Tetrapanacis 8 parts, 3 parts, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 5 times of water, decoct 1 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 1.5 ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 0.5 × 10 to exponential phase clump count
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 2 parts in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 34 DEG C, and fermentation time is 48 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
Embodiment 6:
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow, be made up of the raw material of following weight portion: Herba Taraxaci 14 parts, Herba Violae 12 parts, Flos Lonicerae 5 parts, Rhizoma Chuanxiong 9 parts, Radix Angelicae Sinensis 9 parts, 3 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 12 parts, Medulla Tetrapanacis 9 parts, 3 parts, Radix Glycyrrhizae, bacillus acidophilus 3 parts.
The preparation method of the Chinese herbal medicine fermented extracted liquid of this control mammitis of cow, concrete steps are as follows:
(1) take Herba Taraxaci 14 parts, Herba Violae 12 parts, Flos Lonicerae 5 parts, Rhizoma Chuanxiong 9 parts, Radix Angelicae Sinensis 9 parts, 3 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 12 parts, Medulla Tetrapanacis 9 parts, 3 parts, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 6 times of water, decoct 2 hours, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation in MRS solid medium; In cultured slant strains, add the sterilized normal saline of 3ml, prepare bacteria suspension; The bacteria suspension that slant culture produces is carried out interior anaerobism amplification culture in MRS fluid medium, is 1.0 × 10 to exponential phase clump count
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 3 parts in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and the temperature of fermentation is 37 DEG C, and fermentation time is 36 hours, subpackage, obtains Chinese herbal medicine fermented extracted liquid.
With specific experiment, effect of the present invention is described below:
Trial target: Chinese herbal medicine fermented extracted liquid of the present invention; Reference substance: extracts of Chinese herbal medicine.
Experimental animal: the holstein cow 30 in Stochastic choice plant lactogenic mid-term, is divided into five groups, often organizes six.
EXPERIMENTAL DESIGN: the product of the oral feeding embodiment of the present invention 2 of group one, the product of the oral feeding embodiment of the present invention 3 of group two, the product of the oral feeding embodiment of the present invention 4 of group three, pure extracts of Chinese herbal medicine in the present invention of group four oral feeding is matched group, and it is blank group that group five does not feed any medicine.
Test method: during on-test and at the end of, adopt the somatic number of somatic cell analysis-e/or determining milch cow, each measurement result is the tie-in meansigma methods of two days; Measure the initial milk yield of every cow head during on-test, measured once every 10 days, the test period is two months, calculates each group of average milk production in two months respectively later.
Clinical test results refers to following table:
As can be seen from above result of the test, the detection before overtesting, find that the SCC value of five groups has 2,1,2,3,2 respectively higher than 500,000/ml, illustrate when on-test, this milch cow just has the possibility infecting mastitis; During off-test, find through test, find that the SCC value of group four and group five has 4,6 respectively higher than 500,000/ml, the somatic number of first three group is all than little many during on-test, illustrate that the present invention can reduce the somatic number of milk, have significant effect to control mammitis of cow.
As can be seen from the above tests, group one, two and three at the average daily milk production amplification degree of experimental period all more than more than 10%, milk after test increases than weight average, although group four average daily milk production and milk proportion also have increase, but amplitude is far from, the extracts of Chinese herbal medicine after fermenting is large, although the average daily milk production that group five tests rear milch cow too increases 1.56%, milk proportion increases to negative value; In view of this, Chinese herbal medicine fermented extracted liquid of the present invention significantly can either promote the increase of milk milk amount, can increase milk proportion again, illustrates that the present invention can improve efficiency of feed utilization.
Above-described is only the preferred embodiment of the present invention; should be understood that; for a person skilled in the art; do not departing under general idea prerequisite of the present invention; some changes and improvements can also be made; these also should be considered as protection scope of the present invention, and these all can not affect effect of the invention process and practical applicability.
Claims (5)
1. prevent and treat a Chinese herbal medicine fermented extracted liquid for mammitis of cow, be characterised in that: be made up of the raw material of following weight portion: Herba Taraxaci 10 parts ~ 15 parts, Herba Violae 5 parts ~ 15 parts, Flos Lonicerae 5 parts ~ 10 parts, Rhizoma Chuanxiong 5 parts ~ 12 parts, Radix Angelicae Sinensis 5 parts ~ 10 parts, 1 part ~ 10 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 10 parts ~ 20 parts, Medulla Tetrapanacis 5 parts ~ 10 parts, 1 ~ 3 part, Radix Glycyrrhizae, bacillus acidophilus 1 ~ 3 part.
2. a kind of Chinese herbal medicine fermented extracted liquid preventing and treating mammitis of cow according to claim 1, is characterized in that: be made up of the raw material of following weight portion: Herba Taraxaci 15 parts, Herba Violae 10 parts, Flos Lonicerae 8 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 8 parts, 6 parts, Pericarpium Citri Reticulatae Viride, Semen Vaccariae 18 parts, Medulla Tetrapanacis 7 parts, 1 part, Radix Glycyrrhizae, bacillus acidophilus 2 parts.
3. the Chinese herbal medicine fermented extracted liquid and preparation method thereof of control mammitis of cow according to claim 1, is characterized in that: concrete steps are as follows:
(1) take Herba Taraxaci, Herba Violae, Flos Lonicerae, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Pericarpium Citri Reticulatae Viride, Semen Vaccariae, Medulla Tetrapanacis, Radix Glycyrrhizae, decoct with water 3 times, each chemical feeding quantity 5 ~ 6 times of water, decoct 1 ~ 2 hour, filter, merging filtrate, cooling;
(2) Lactobacillus acidophilus species is carried out slant strains cultivation; In cultured slant strains, add the sterilized normal saline of 1 ~ 3ml, prepare bacteria suspension; By bacteria suspension anaerobism amplification culture in the culture medium of correspondence that slant culture produces, be 1.0 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
10during CFU/ml only, bacillus acidophilus bacterium liquid is obtained;
(3) get bacillus acidophilus bacterium liquid 1 ~ 3 part in mass ratio respectively, add in 50 parts of step (1) gained Chinese herbal medicine filtrate, closing anaerobic ferments, and subpackage, obtains Chinese herbal medicine fermented extracted liquid.
4. a kind of Chinese herbal medicine fermented extracted liquid and preparation method thereof for preventing and treating piglet pujos blancos according to claim 3, it is characterized in that: the culture medium of carrying out slant strains cultivation described in step (2) is MRS solid medium, the culture medium of carrying out anaerobism amplification culture is MRS fluid medium.
5. a kind of Chinese herbal medicine fermented extracted liquid and preparation method thereof for preventing and treating piglet pujos blancos according to claim 4, it is characterized in that: described in step (3), the temperature of anaerobic fermentation is 30 ~ 37 DEG C, fermentation time is 24 ~ 48 hours.
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| CN106676020A (en) * | 2015-11-06 | 2017-05-17 | 中创云牧科技咨询(北京)股份有限公司 | Application of lactic acid bacteria in modulating silage and preventing dairy cow mastitis |
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| CN108743739A (en) * | 2018-06-27 | 2018-11-06 | 张家口万全区科泰生物科技有限公司 | A kind of preparation method of the active peptide of enhancing milk cow immunity |
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