CN104474006A - Traditional Chinese medicine microecological preparation capable of increasing lactation yield of dairy cow and preparation method thereof - Google Patents

Traditional Chinese medicine microecological preparation capable of increasing lactation yield of dairy cow and preparation method thereof Download PDF

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CN104474006A
CN104474006A CN201410702695.8A CN201410702695A CN104474006A CN 104474006 A CN104474006 A CN 104474006A CN 201410702695 A CN201410702695 A CN 201410702695A CN 104474006 A CN104474006 A CN 104474006A
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chinese medicine
radix
preparation
dairy cow
bacterium liquid
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李凤娟
刘伟
朱丽平
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Zhengzhou Houyi Pharmaceutical Co Ltd
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Zhengzhou Houyi Pharmaceutical Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/744Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747Lactobacilli, e.g. L. acidophilus or L. brevis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/742Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/062Ascomycota
    • A61K36/064Saccharomycetales, e.g. baker's yeast
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/23Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232Angelica
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/23Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236Ligusticum (licorice-root)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/34Campanulaceae (Bellflower family)
    • A61K36/344Codonopsis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/36Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481Astragalus (milkvetch)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/483Gleditsia (locust)

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Abstract

The invention relates to a traditional Chinese medicine microecological preparation capable of increasing the lactation yield of dairy cow. The traditional Chinese medicine microecological preparation is obtained by adding lactobacillus, saccharomycetes and bacillus subtilis in the extract of traditional Chinese medicines, and carrying out airtight anaerobic fermentation, wherein the traditional Chinese medicines are composed of the following components in parts by weight: 10-25 parts of cowherb seed, 5-15 parts of astragalus membranaceus, 5-20 parts of spina gleditsiae, 10-25 parts of angelica sinensis, 5-20 parts of codonopsis pilosula, 10-30 parts of ligusticum wallichii, 1-8 parts of uniflower swisscentaury root and 1-6 parts of beautiful sweetgum fruit. By using the traditional Chinese medicine microecological preparation disclosed by the invention, the butter-fat percentage and the milk proportion can be increased, the yield of mycoproteins can be increased, the animal immunity can be enhanced, the feed utilization rate can be increased, and the milk yield of the dairy cow is finally increased.

Description

A kind ofly improve Chinese medicine microecological preparation of Lactation of Dairy Cow amount and preparation method thereof
Technical field
The invention belongs to veterinary drug technical field, be specifically related to a kind ofly improve Chinese medicine microecological preparation of Lactation of Dairy Cow amount and preparation method thereof.
Background technology
In recent years, China's milk cow production fast development, but along with the raising of people's living standard and the raising to Animal product quality requirement, the pollution-free food that antibiotic-free remains becomes the focus that people pay close attention to, and milk and milk product thereof especially come into one's own.In recent years, utilize Chinese medicine microecological preparation to adjust or maintain microecological balance in animal intestinal, enhancing human body immunity function, what promote nutrient substance digests and assimilates the focus becoming Animal nutrition research.Chinese medicine plays the effect of disease therapy and prevention and health care in breeding process, the Chinese medicine undergone microbial fermentation, i.e. Chinese medicine microecological preparation, Chinese medicine ingredients wherein transforms through probiotic bacteria, micromolecule is become by macromole, rapid-action, drug effect is high, 100% absorbed by body, is applied in the production cultivation of milch cow, to improving milk yield, improve the generation of milk quality and prevention bovine subclinical mastitis and have positive role.It not only has nutrition health-care functions, but also avoids the veterinary drugs such as antibacterials in animal body residual, is also a class Green Product.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine microecological preparation improving Lactation of Dairy Cow amount, this Chinese medicine microecological preparation can improve milk yield, improves milk quality and prevent the generation of bovine subclinical mastitis to have positive role.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of Chinese medicine microecological preparation improving Lactation of Dairy Cow amount, be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 10 ~ 25, the Radix Astragali 5 ~ 15, Spina Gleditsiae 5 ~ 20, Radix Angelicae Sinensis 10 ~ 25, Radix Codonopsis 5 ~ 20, Rhizoma Chuanxiong 10 ~ 30, Radix Rhapontici 1 ~ 8 and Fructus Liquidambaris 1 ~ 6.
The Chinese medicine microecological preparation of described raising Lactation of Dairy Cow amount, be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 15 ~ 20, the Radix Astragali 8 ~ 10, Spina Gleditsiae 7 ~ 10, Radix Angelicae Sinensis 15 ~ 20, Radix Codonopsis 5 ~ 10, Rhizoma Chuanxiong 10 ~ 20, Radix Rhapontici 1 ~ 5 and Fructus Liquidambaris 1 ~ 5.
The Chinese medicine microecological preparation of described raising Lactation of Dairy Cow amount, be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 20, the Radix Astragali 10, Spina Gleditsiae 10, Radix Angelicae Sinensis 20, Radix Codonopsis 10, Rhizoma Chuanxiong 20, Radix Rhapontici 5 and Fructus Liquidambaris 5.
Improve a preparation method for the Chinese medicine microecological preparation of Lactation of Dairy Cow amount, concrete steps are as follows:
(1) Chinese medicine Semen Vaccariae, the Radix Astragali, Spina Gleditsiae, Radix Angelicae Sinensis, Radix Codonopsis, Rhizoma Chuanxiong, Radix Rhapontici and Fructus Liquidambaris being dried, pulverizing is that 100 order powder are for subsequent use;
(2) extracting lactic acid bacillus, yeast and bacillus subtilis carry out slant strains cultivation respectively as strain, and the suspension produced by often kind of slant culture is difference amplification culture in appropriate media, is cultured to exponential phase 0.5 × 10 8cFU/mL to 1.0 × 10 8during CFU/mL only, for subsequent use;
(3) preparation of mixed bacteria liquid: be 1:(20 ~ 30 according to the weight ratio of lactobacillus bacterium liquid and powder) measure lactobacillus bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of yeast bacterium liquid and powder) measure yeast bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of bacillus subtilis bacterium liquid and powder) measure bacillus subtilis bacterium liquid, lactobacillus bacterium liquid, yeast bacterium liquid and bacillus subtilis bacterium liquid are mixed to get mixed bacteria liquid;
(4) measuring Chinese medicinal powder by formula ratio adds in the mixed bacteria liquid of step (3), anaerobic fermentation 36 ~ 72 hours at 30 ~ 37 DEG C, and through lyophilization, subpackage, must improve the Chinese medicine microecological preparation of Lactation of Dairy Cow amount.
the present invention selects effect of raw material as follows:
Semen Vaccariae: have promoting blood circulation to restore menstrual flow, reducing swelling and alleviating pain, expedites the emergence of the function of stimulating milk secretion.
The Radix Astragali: there is the effects such as invigorating QI to consolidate the body surface resistance, diuretic detumescent, expelling pus and toxin by strengthening QI, granulation promoting.
Spina Gleditsiae: cure mainly swollen ulcer drug, scrofula, skin ulcer rash stubborn dermatitis, puerperal hypogalactia, placenta retention, leprosy.
Radix Angelicae Sinensis: enrich blood; Invigorate blood circulation; Menstruction regulating and pain relieving; Moisturize laxation.
Radix Codonopsis: there is invigorating the spleen and replenishing QI, effect of spleen invigorating lung benefiting.
Rhizoma Chuanxiong: blood-activating and qi-promoting, wind-expelling pain-stopping.
Radix Rhapontici: heat-clearing and toxic substances removing, eliminating carbuncle, stimulating milk secretion, relaxing muscles and tendons is promoted blood circulation.
Fructus Liquidambaris: dispelling, collateral-activating, diuretic stimulates the menstrual flow.For arthralgia, numbness contracture, edema distension, the few amenorrhea of breast.
Lactobacillus, yeast and bacillus subtilis: be supplemented with bacteria group, maintain microecological balance; Nutrition digestion-aid effect; Improve immunity of organisms; Environment purification.
compared with prior art, the beneficial effect that obtains of the present invention:
1. Chinese medicine microecological preparation of the present invention improves butterfat percnetage and milk proportion, increases the output of tropina, strengthens animal immunizing power, improves efficiency of feed utilization, the final milk yield improving milch cow.
2. Chinese medicine ingredients of the present invention transforms through probiotic bacteria, micromolecule is become by macromole, rapid-action, drug effect is high, 100% absorbed by body, is applied in the production cultivation of milch cow, to improving milk yield, improve the generation of milk quality and prevention bovine subclinical mastitis and have positive role.
3. Chinese medicine microecological preparation of the present invention not only has nutrition health-care functions, but also avoids the veterinary drugs such as antibacterials in animal body residual, is also a class Green Product.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but do not limit content of the present invention.
Following bacterium liquid self-control or commercially available:
embodiment 1
A kind of Chinese medicine microecological preparation improving Lactation of Dairy Cow amount, be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 10 ~ 25, the Radix Astragali 5 ~ 15, Spina Gleditsiae 5 ~ 20, Radix Angelicae Sinensis 10 ~ 25, Radix Codonopsis 5 ~ 20, Rhizoma Chuanxiong 10 ~ 30, Radix Rhapontici 1 ~ 8 and Fructus Liquidambaris 1 ~ 6.
Improve a preparation method for the Chinese medicine microecological preparation of Lactation of Dairy Cow amount, concrete steps are as follows:
(1) Chinese medicine Semen Vaccariae, the Radix Astragali, Spina Gleditsiae, Radix Angelicae Sinensis, Radix Codonopsis, Rhizoma Chuanxiong, Radix Rhapontici and Fructus Liquidambaris being dried, pulverizing is that 100 order powder are for subsequent use;
(2) extracting lactic acid bacillus, yeast and bacillus subtilis carry out slant strains cultivation respectively as strain, the suspension produced by often kind of slant culture is difference amplification culture in appropriate media, stop when being cultured to exponential phase 0.5 × 108CFU/mL to 1.0 × 108CFU/mL, for subsequent use;
(3) preparation of mixed bacteria liquid: be 1:(20 ~ 30 according to the weight ratio of lactobacillus bacterium liquid and powder) measure lactobacillus bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of yeast bacterium liquid and powder) measure yeast bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of bacillus subtilis bacterium liquid and powder) measure bacillus subtilis bacterium liquid, lactobacillus bacterium liquid, yeast bacterium liquid and bacillus subtilis bacterium liquid are mixed to get mixed bacteria liquid;
(4) measuring Chinese medicinal powder by formula ratio adds in the mixed bacteria liquid of step (3), anaerobic fermentation 36 ~ 72 hours at 30 ~ 37 DEG C, and through lyophilization, subpackage, must improve the Chinese medicine microecological preparation of Lactation of Dairy Cow amount, spice feed.
embodiment 2
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 20, the Radix Astragali 10, Spina Gleditsiae 10, Radix Angelicae Sinensis 20, Radix Codonopsis 10, Rhizoma Chuanxiong 20, Radix Rhapontici 5 and Fructus Liquidambaris 5.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 3
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 15, the Radix Astragali 8, Spina Gleditsiae 8, Radix Angelicae Sinensis 12, Radix Codonopsis 7, Rhizoma Chuanxiong 15, Radix Rhapontici 3 and Fructus Liquidambaris 2.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 4
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 10, the Radix Astragali 5, Spina Gleditsiae 5, Radix Angelicae Sinensis 10, Radix Codonopsis 5, Rhizoma Chuanxiong 10, Radix Rhapontici 1 and Fructus Liquidambaris 1.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 5
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 12, the Radix Astragali 7, Spina Gleditsiae 7, Radix Angelicae Sinensis 22, Radix Codonopsis 8, Rhizoma Chuanxiong 16, Radix Rhapontici 8 and Fructus Liquidambaris 4.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 6
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 20, the Radix Astragali 13, Spina Gleditsiae 18, Radix Angelicae Sinensis 11, Radix Codonopsis 20, Rhizoma Chuanxiong 25, Radix Rhapontici 2 and Fructus Liquidambaris 3.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 7
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 22, the Radix Astragali 8, Spina Gleditsiae 9, Radix Angelicae Sinensis 20, Radix Codonopsis 8, Rhizoma Chuanxiong 19, Radix Rhapontici 3 and Fructus Liquidambaris 6.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 8
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 25, the Radix Astragali 15, Spina Gleditsiae 10, Radix Angelicae Sinensis 14, Radix Codonopsis 16, Rhizoma Chuanxiong 18, Radix Rhapontici 8 and Fructus Liquidambaris 2.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 9
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 16, the Radix Astragali 12, Spina Gleditsiae 17, Radix Angelicae Sinensis 16, Radix Codonopsis 6, Rhizoma Chuanxiong 30, Radix Rhapontici 4 and Fructus Liquidambaris 4.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 10
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 15, the Radix Astragali 9, Spina Gleditsiae 7, Radix Angelicae Sinensis 15, Radix Codonopsis 5, Rhizoma Chuanxiong 10, Radix Rhapontici 5 and Fructus Liquidambaris 5.
The present embodiment is identical with embodiment 1 preparation method.
embodiment 11
Improve a Chinese medicine microecological preparation for Lactation of Dairy Cow amount, described Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 20, the Radix Astragali 8, Spina Gleditsiae 8, Radix Angelicae Sinensis 19, Radix Codonopsis 7, Rhizoma Chuanxiong 14, Radix Rhapontici 3 and Fructus Liquidambaris 2.
The present embodiment is identical with embodiment 1 preparation method.
With specific experiment, effect of the present invention is described below:
1 materials and methods:
1.1 trial targets: add feedstuff of the present invention.Reference substance: normal diet
1.2 experimental animals: holstein cow
1.3 EXPERIMENTAL DESIGN
Each 30 of the holstein cow of Stochastic choice 11 plants.The product of the plant 1 spice feeding embodiment of the present invention 2, the product of the plant 2 spice feeding embodiment of the present invention 3, the product of the plant 3 spice feeding embodiment of the present invention 4, the like, the pure feedstuff of plant 11 spice feeding.
1.4 test method
Test first 10 days, the milk that the milch cow every of front 10 plants and the 11st plant produces every day is weighed and gravity test, to front 10 plants spice embodiment of the present invention 2 ~ 11 product respectively before from the 11st day, the 11st plant's feed normal diet.Continuous Observation 20 days, records the milk yield of every cow head every in 20 days and proportion thereof in table 1.
1.5 clinical test results
Table 1 pair plant 1 ~ 11 test data sheet result
As can be seen from the above tests, plant 1 ~ 10 is at the average daily milk production amplification degree of experimental period all more than more than 10%, and the milk after test increases than weight average.The average daily milk production that rear milch cow is tested by plant 11 increases by 3.05%, and milk proportion increases to negative value.In view of this, Chinese medicine microorganism formulation of the present invention can promote the increase of milk milk amount.While milk amount increases, milk proportion does not decline, and explanation is the effect that Chinese medicine microecological preparation improves efficiency of feed utilization.

Claims (4)

1. one kind is improved the Chinese medicine microecological preparation of Lactation of Dairy Cow amount, it is characterized in that: be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 10 ~ 25, the Radix Astragali 5 ~ 15, Spina Gleditsiae 5 ~ 20, Radix Angelicae Sinensis 10 ~ 25, Radix Codonopsis 5 ~ 20, Rhizoma Chuanxiong 10 ~ 30, Radix Rhapontici 1 ~ 8 and Fructus Liquidambaris 1 ~ 6.
2. improve the Chinese medicine microecological preparation of Lactation of Dairy Cow amount according to claim 1, it is characterized in that: be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 15 ~ 20, the Radix Astragali 8 ~ 10, Spina Gleditsiae 7 ~ 10, Radix Angelicae Sinensis 15 ~ 20, Radix Codonopsis 5 ~ 10, Rhizoma Chuanxiong 10 ~ 20, Radix Rhapontici 1 ~ 5 and Fructus Liquidambaris 1 ~ 5.
3. improve the Chinese medicine microecological preparation of Lactation of Dairy Cow amount according to claim 1, it is characterized in that: be add the fermentation of lactobacillus, yeast and bacillus subtilis closing anaerobic by the extracting solution of Chinese medicine to form, wherein said Chinese medicine is made up of the component of following weight portion: Semen Vaccariae 20, the Radix Astragali 10, Spina Gleditsiae 10, Radix Angelicae Sinensis 20, Radix Codonopsis 10, Rhizoma Chuanxiong 20, Radix Rhapontici 5 and Fructus Liquidambaris 5.
4. improve a preparation method for the Chinese medicine microecological preparation of Lactation of Dairy Cow amount as claimed in claim 1, it is characterized in that, concrete steps are as follows:
(1) Chinese medicine Semen Vaccariae, the Radix Astragali, Spina Gleditsiae, Radix Angelicae Sinensis, Radix Codonopsis, Rhizoma Chuanxiong, Radix Rhapontici and Fructus Liquidambaris being dried, pulverizing is that 100 order powder are for subsequent use;
(2) extracting lactic acid bacillus, yeast and bacillus subtilis carry out slant strains cultivation respectively as strain, and the suspension produced by often kind of slant culture is difference amplification culture in appropriate media, is cultured to exponential phase 0.5 × 10 8cFU/mL to 1.0 × 10 8during CFU/mL only, for subsequent use;
(3) preparation of mixed bacteria liquid: be 1:(20 ~ 30 according to the weight ratio of lactobacillus bacterium liquid and powder) measure lactobacillus bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of yeast bacterium liquid and powder) measure yeast bacterium liquid, be 1:(20 ~ 30 according to the weight ratio of bacillus subtilis bacterium liquid and powder) measure bacillus subtilis bacterium liquid, lactobacillus bacterium liquid, yeast bacterium liquid and bacillus subtilis bacterium liquid are mixed to get mixed bacteria liquid;
(4) measuring Chinese medicinal powder by formula ratio adds in the mixed bacteria liquid of step (3), anaerobic fermentation 36 ~ 72 hours at 30 ~ 37 DEG C, and through lyophilization, subpackage, must improve the Chinese medicine microecological preparation of Lactation of Dairy Cow amount.
CN201410702695.8A 2014-11-29 2014-11-29 Traditional Chinese medicine microecological preparation capable of increasing lactation yield of dairy cow and preparation method thereof Pending CN104474006A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
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CN105341377A (en) * 2015-11-20 2016-02-24 衡阳市富矿饲料添加剂有限公司 Feed additive for improving milk production rate of cows, and preparation method thereof
CN105661025A (en) * 2016-01-08 2016-06-15 四川农业大学 Feed additive capable of reducing somatic cell account of dairy cow and preparation method thereof
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CN115998790A (en) * 2021-10-22 2023-04-25 洛阳瑞华动物保健品有限公司 Traditional Chinese medicine preparation for improving lactation of female animals

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Publication number Priority date Publication date Assignee Title
CN105341377A (en) * 2015-11-20 2016-02-24 衡阳市富矿饲料添加剂有限公司 Feed additive for improving milk production rate of cows, and preparation method thereof
CN105661025A (en) * 2016-01-08 2016-06-15 四川农业大学 Feed additive capable of reducing somatic cell account of dairy cow and preparation method thereof
CN108653518A (en) * 2018-05-24 2018-10-16 湖北华大瑞尔科技有限公司 A kind of probiotics fermention Chinese medicine preparation and preparation method thereof
CN108653518B (en) * 2018-05-24 2021-10-08 湖北华大瑞尔科技有限公司 Probiotic fermented traditional Chinese medicine preparation and preparation method thereof
CN115998790A (en) * 2021-10-22 2023-04-25 洛阳瑞华动物保健品有限公司 Traditional Chinese medicine preparation for improving lactation of female animals
CN115998790B (en) * 2021-10-22 2024-02-09 洛阳瑞华动物保健品有限公司 Traditional Chinese medicine preparation for improving lactation of female animals

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