CN114848763B - Traditional Chinese medicine composition for sow gestation period and/or lactation period, fermented traditional Chinese medicine preparation and preparation method thereof - Google Patents

Traditional Chinese medicine composition for sow gestation period and/or lactation period, fermented traditional Chinese medicine preparation and preparation method thereof Download PDF

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CN114848763B
CN114848763B CN202210306459.9A CN202210306459A CN114848763B CN 114848763 B CN114848763 B CN 114848763B CN 202210306459 A CN202210306459 A CN 202210306459A CN 114848763 B CN114848763 B CN 114848763B
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chinese medicine
traditional chinese
medicine composition
bacterial liquid
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CN114848763A (en
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乔宏兴
范明夏
孙彦婷
张晓静
康宇
赵圣振
张菡
边传周
邢得强
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Henan University of Animal Husbandry and Economy
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
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    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/533—Leonurus (motherwort)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/638—Ligustrum, e.g. Chinese privet
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8998—Hordeum (barley)
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract

The invention provides a traditional Chinese medicine composition for a sow in gestation and/or lactation, a fermented traditional Chinese medicine preparation and a preparation method thereof, and belongs to the technical field of fermented traditional Chinese medicine preparations for animals. The invention relates to a traditional Chinese medicine composition for sows in gestation and/or lactation, which comprises the following traditional Chinese medicine components in parts by weight: 60-70 parts of astragalus, 40-50 parts of angelica, 55-65 parts of dangshen, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of poria cocos, 40-50 parts of prepared rehmannia root, 30-40 parts of motherwort, 30-40 parts of glossy privet fruit, 30-40 parts of malt, 30-40 parts of dried orange peel and 30-40 parts of liquorice. The invention relates to a fermented traditional Chinese medicine preparation for sows in gestation and/or lactation, which is mainly prepared from lactic acid bacteria and the traditional Chinese medicine composition; the lactobacillus consists of lactobacillus plantarum and enterococcus faecium, and the probiotic and the traditional Chinese medicine composition are organically combined, so that the sub-health state of the sow can be improved, and the production performance of the sow can be improved.

Description

Traditional Chinese medicine composition for sow gestation period and/or lactation period, fermented traditional Chinese medicine preparation and preparation method thereof
Technical Field
The invention belongs to the technical field of fermented traditional Chinese medicine preparations for livestock, and particularly relates to a traditional Chinese medicine composition for sows in gestation and/or lactation, a fermented traditional Chinese medicine preparation and a preparation method thereof.
Background
The sow is the most core 'production machine' of the pig farm, the health condition of the sow is directly related to the exertion of the production performance and influences the production benefit of the pig farm, and particularly, the large-scale and intensive cultivation mode and the high-strength production rhythm in the modern animal husbandry cultivation bring higher requirements to the health level of the sow. The epidemic disease generated by livestock in traditional breeding is often treated by antibiotics, but with the development of modern animal husbandry, the drug resistance problem and toxic and side effects caused by the antibiotics are also gradually highlighted, the long-term taking of the livestock can not only have adverse effects on the health of the livestock, but also cause the occurrence of food safety problem due to drug residues in the meat quality of the livestock, so that more pig farms begin to use no antibiotics.
However, in actual clinic, the problem that the sow has a plurality of sub-health problems such as coarse and disordered fur, eye droppings and tear spots, constipation, poor resistance and the like after no antibiotics are used is found, and the production performance of the sow is reduced, so that the pig farm benefit is influenced. Therefore, a green pollution-free drug capable of replacing antibiotics is needed to improve the sub-health condition of sows and improve the productivity of the sows.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for sows in gestation and/or lactation, and the prepared fermented traditional Chinese medicine preparation can improve sub-health problems of sows in gestation and lactation.
The second aim of the invention is to provide a fermented traditional Chinese medicine preparation for sows in gestation and/or lactation, which is beneficial to improving the sub-health problems of sows in gestation and lactation.
The third aim of the invention is to provide a preparation method of the fermented traditional Chinese medicine preparation for the gestation period and/or the lactation period of the sow, which has the advantages of simple process and high utilization rate.
The invention relates to a traditional Chinese medicine composition for sows in gestation and/or lactation, which adopts the following technical scheme:
a traditional Chinese medicine composition for sows in gestation and/or lactation comprises the following traditional Chinese medicine components in parts by weight: 60-70 parts of astragalus, 40-50 parts of angelica, 55-65 parts of dangshen, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of poria cocos, 40-50 parts of prepared rehmannia root, 30-40 parts of motherwort, 30-40 parts of glossy privet fruit, 30-40 parts of malt, 30-40 parts of dried orange peel and 30-40 parts of liquorice.
The invention relates to a traditional Chinese medicine composition for sows in gestation and/or lactation, which comprises the following characteristics:
radix astragali: is dry root of Astragalus membranaceus (Fisch.) bge, astragalus mongholicus (Fisch.) bge, or other similar plants of Leguminosae, contains polysaccharides such as glucose AG-1, AG-2 and heteropolysaccharide AH-1, AH-2, saponins such as Astragalus saponins I-VIII, soyasaponin I, astragaloside IV and Astragaloside IV etc. as effective components; slightly warm and sweet in nature and taste; enter lung and spleen meridians; has the effects of invigorating qi, strengthening yang, consolidating superficial resistance, stopping sweating, promoting wound healing, promoting granulation, promoting diuresis and relieving swelling. Is mainly used for treating spleen and lung qi deficiency, qi deficiency sinking, exterior deficiency sweating, sore and carbuncle difficult to be ulcerated and qi deficiency edema. Modern pharmacology proves that astragalus has the functions of enhancing immunity, promoting hematopoiesis, improving gastrointestinal tract function, regulating heart, regulating blood pressure, resisting tumor, resisting radiation, resisting fatigue, resisting oxidative damage and protecting liver.
Chinese angelica root: is root of Diels of Angelica of Umbelliferae, angelica Angelica sinensis (Oliv.) containing neutral oil component such as beta-pinene, alpha-pinene, camphene, etc. Comprises effective components such as acid oil component such as p-methyl benzyl alcohol, 5-methoxy-2, 3-xylenol, organic acid, saccharide, vitamins, amino acids, etc.; sweet and pungent taste and warm nature; enter liver, heart and spleen meridians; has effects of replenishing blood, regulating menstruation, promoting blood circulation, relieving pain, loosening bowel, and relieving constipation. Is used for treating sallow complexion due to blood deficiency, menoxenia, amenorrhea, dysmenorrhea, blood deficiency, blood stagnation, pain due to blood cold, traumatic injury, rheumatalgia, carbuncle, skin ulcer, constipation due to intestinal dryness, etc. Modern pharmacology proves that angelica has the effects of resisting anemia, enhancing immunity, inhibiting platelet aggregation, resisting thrombus, resisting myocardial ischemia and hypoxia, expanding peripheral blood vessels, reducing blood pressure, exciting or inhibiting uterine smooth muscle, relaxing bronchial smooth muscle, reducing blood fat, resisting inflammation, protecting liver and the like.
Radix codonopsis pilosulae: is dried root of radix Codonopsis Codonopsis pilosula (Franch.) belonging to Campanulaceae, nannf, nannf.var.modesta (Nannf.) L.T.shen or radix Codonopsis Codonopsis tangshen Oliv. Containing volatile oil, baicalin glucoside, trace alkaloid, amino acid, polysaccharide and saponin, and containing syringin, n-hexyl-beta-D-glucopyranoside, dandelion alcohol, friedelane, etc.; sweet in nature and smell, and flat; spleen and lung meridian entered; has the effects of invigorating spleen and replenishing qi, and invigorating spleen and replenishing lung. Is mainly used for treating weakness of spleen and stomach, diarrhea caused by overeating, tiredness and weakness, cough and asthma caused by deficiency of lung, and the like. Modern pharmacological researches have proved that radix Codonopsis has effects of lowering blood pressure, exciting nervous system, increasing blood sugar and promoting blood coagulation.
White atractylodes rhizome: is dried rhizome of Atractylodes macrocephala Koidz of Atractylodes of Compositae, and contains atractylol, beta-eucalyptol, atractylol, atractylis ovata ether, atractylis ovata lactone, dehydrated atractylis ovata lactone, fructose, inulin, etc. as effective components; warm, sweet and bitter in nature and taste; spleen and stomach meridian; has the effects of invigorating spleen and replenishing qi, eliminating dampness and promoting diuresis, consolidating superficial resistance and arresting sweating, tonifying qi and preventing miscarriage. Is used for treating spleen deficiency diarrhea, edema, spontaneous perspiration, and fetal irritability. Modern pharmacology proves that the bighead atractylodes rhizome has the effects of improving gastrointestinal functions, enhancing immune system functions, resisting tumors, promoting urination, regulating nervous systems and the like.
Poria cocos: is dry sclerotium of Poria cocos (Polyporaceae) and contains effective components such as beta-pachyman, pachymic acid, acetylpachymic acid, melic acid, apopore acid, 3 beta-hydroxy wool-supported trienoic acid, histidine, choline, adenine, etc.; the taste is flat, sweet and light; return to heart, lung, spleen, stomach and kidney meridians; has effects of promoting diuresis, removing dampness, invigorating spleen, invigorating middle warmer, and tranquilizing mind. Is used for treating phlegm dampness edema, oliguria, spleen deficiency, anorexia, loose stool, diarrhea, and restlessness. Modern pharmacology proves that poria cocos has the effects of promoting diuresis, soothing nerves, resisting inflammation and bacteria, resisting tumors, improving immunity, protecting liver, resisting rejection reaction, promoting hematopoiesis and the like.
Prepared rehmannia root: is root tuber of rehmannia (academic name: rehmannia glutinosa) belonging to Scrophulariaceae, and contains rehmannia glycoside, rehmannia essence, jiao Dehuang essence, and rehmannia kudzein. Sweet in nature and slightly warm. It enters liver and kidney meridians. Has effects of replenishing blood, nourishing yin, replenishing essence, and nourishing marrow. It is mainly used for treating blood deficiency, asthenia, blood deficiency, essence deficiency, deficiency heat, night sweat, etc. Modern pharmacological researches have proved that the prepared rehmannia root has the effects of reducing blood sugar content, strengthening heart, promoting urination and inhibiting fungi.
Motherwort: is fresh or dried aerial parts of herba Leonuri Leonurus japonicus Houttuynt of Leonurus of Labiatae, and contains leonurine, stachydrine, linolenic acid, beta-linolenic acid, oleic acid, lauric acid, benzoic acid, rutin, and fumaric acid. Bitter and pungent in flavor, slightly cold in nature, entering liver, pericardium and bladder meridians. Has effects of promoting blood circulation, regulating menstruation, inducing diuresis, relieving swelling, and clearing heat and toxic substances. It is used for treating menoxenia, dysmenorrhea, amenorrhea, lochiorrhea, edema, oliguria, and pyocutaneous disease. Modern pharmacological researches have proved that motherwort has the effects of stimulating uterus, accelerating uterine contraction, promoting urination and inhibiting dermatophytes.
Fructus ligustri lucidi: is mature fruit of Ligustrum lucidum ait Ligustrum lucidum Ait. Belonging to Oleaceae, and contains oleanolic acid, acetyl oleanolic acid, ursolic acid, mannitol, glucose, palmitic acid, stearic acid, oleic acid, linoleic acid, etc. Sweet and bitter in nature and cool in nature; it enters liver and kidney meridians. Has effects of nourishing liver and kidney, clearing heat and improving eyesight. Is used for treating soreness of waist and knees, premature graying of beard and hair, internal heat due to yin deficiency, fatigue heat due to bone steaming, night sweat, spermatorrhea, dizziness, dim eyesight, amnesia, tinnitus, etc. Modern pharmacological researches have proved that fructus Ligustri Lucidi has anticancer, antiinflammatory, blood lipid reducing, blood glucose reducing, immunity enhancing, HPD photooxidation resisting, intraocular pressure reducing, antiaging, and liver protecting effects.
Malt: is obtained by germinating and drying mature fruit of Hordeum vulgare L. Belonging to Gramineae, and contains amylase, invertase, vitamin C, fat, lecithin, dextrin, maltose, glucose, etc. Sweet in nature and flat in nature, enters spleen and stomach meridians. Has effects of promoting qi circulation, resolving food stagnation, invigorating spleen, stimulating appetite, and promoting lactation. Is mainly used for treating weakness of spleen and stomach, inappetence, abdominal distention, milk stasis, etc. Modern pharmacological studies prove that malt has the effects of promoting secretion of gastric acid and pepsin, reducing blood sugar and inhibiting platelet aggregation.
Dried orange peel: is dried mature pericarp of Citrus reticulata Citrus reticulata Blanco and its cultivar of Citrus of Rutaceae, and contains nobiletin, hesperidin, neohesperidin, hesperetin, p-hydroxyefrin, flavone compounds, etc. Pungent and bitter in nature and warm in nature; enter spleen and lung meridians. Has effects of regulating qi-flowing, invigorating spleen, eliminating dampness and resolving phlegm. It is used for treating spleen and stomach qi stagnation syndrome, emesis, singultus, wet phlegm, cough due to cold phlegm, and thoracic obstruction. Modern pharmacological researches prove that the dried orange peel has the effects of scavenging oxygen free radicals, resisting lipid peroxidation, promoting bile flow, reducing serum cholesterol and the like.
Licorice root: is prepared from dry root and rhizome of Glycyrrhiza Glycyrrhiza uralensis Fisch. Glycyrrhiza Glycyrrhiza inflata Batal. Or Glycyrrhiza glabra Glycyrrhiza glabra L. Belonging to Glycyrrhiza genus of Leguminosae family, and contains glycyrrhizic acid, glycyrrhetinic acid, isoglycyrrhizic acid, glycyrrhizin, mannitol, glucose, sucrose, organic acid, volatile oil, etc. Sweet in taste and neutral in nature; it enters heart, lung, spleen and stomach meridians. Has effects in invigorating qi, invigorating spleen, moistening lung, relieving cough, clearing away heat and toxic materials, relieving spasm, and alleviating pain. Is used for treating spleen deficiency, listlessness, palpitation, cough, asthma, carbuncle, pharyngitis, poisoning due to food and medicine, and spasm and pain of limbs. Modern pharmacological studies prove that licorice has the effects of detoxification, antidiuretic, antitussive, anti-inflammatory and antiallergic, etc.
The traditional Chinese medicine composition for the gestation period and/or the lactation period of the sow has reasonable compatibility of the traditional Chinese medicine components, can exert the mutual synergistic effect, is beneficial to enhancing the immunity of the sow, protecting the reproductive system, promoting the absorption and utilization of nutrition, and simultaneously is beneficial to the growth and development of piglets, can be matched with strains to prepare a fermented traditional Chinese medicine preparation, and has a higher application prospect.
Preferably, the traditional Chinese medicine composition for the gestation period and/or the lactation period of the sow comprises the following traditional Chinese medicine components in parts by weight: 65 parts of astragalus, 45 parts of angelica, 60 parts of radix codonopsis, 60 parts of bighead atractylodes rhizome, 60 parts of poria cocos, 45 parts of prepared rehmannia root, 35 parts of motherwort herb, 35 parts of glossy privet fruit, 35 parts of malt, 35 parts of dried orange peel and 35 parts of liquorice.
The invention relates to a fermented traditional Chinese medicine preparation for sows in gestation and/or lactation, which adopts the following technical scheme:
a fermented Chinese medicinal preparation for sow gestation and/or lactation is mainly prepared from lactobacillus and any one of the above Chinese medicinal compositions; the lactobacillus consists of lactobacillus plantarum and enterococcus faecium.
The invention relates to a fermented traditional Chinese medicine preparation for sows in gestation and/or lactation, which adopts the following main characteristics of lactobacillus:
lactobacillus plantarum: is Lactobacillus in Lactobacillus family, facultative anaerobic, and has optimal pH of 6.5; the strain is in a short rod shape or a chain shape, does not produce spores, and is an off-white, round, smooth and opaque colony in an LBS agar culture medium; lactic acid is produced during fermentation, and the ability to ferment carbohydrates is strong. The main functions are maintaining balance of intestinal flora and intestinal function, promoting nutrient absorption, enhancing immunity, resisting bacteria and tumor, reducing cholesterol, improving liver function, etc.
Enterococcus faecium: the enterococcus is facultative anaerobic gram-positive lactobacillus, the thallus is spherical or chain, has no capsule or spore, has strong environmental adaptability and resistance, can resist various antibiotics such as tetracycline, kanamycin, gentamicin and the like, and has no strict requirement on growth conditions. Arginine can be used as an energy source to ferment sorbitol and not to ferment arabinose. The main functions are improving intestinal microecological balance, preventing and treating animal intestinal flora disorder, decomposing protein into small peptide, synthesizing B vitamins, etc.
The invention selects astragalus, angelica, dangshen, bighead atractylodes rhizome, poria cocos, prepared rhizome of rehmannia, motherwort, glossy privet fruit, malt and dried orange peel as fermentation raw materials, and simultaneously utilizes lactobacillus plantarum and enterococcus faecium to carry out bioconversion to the raw materials to prepare the fermentation traditional Chinese medicine preparation. The invention can fully extract the effective components of the traditional Chinese medicine composition by fermenting the lactobacillus plantarum and the enterococcus faecium, thereby being beneficial to improving the utilization rate of medicinal materials; and the probiotics and the traditional Chinese medicine composition are organically combined, so that the novel efficacy of the fermented traditional Chinese medicine preparation can be given while the efficacy of the traditional Chinese medicine composition is enhanced and toxic and side effects are reduced, the immunity of animal organisms can be improved, the growth is promoted, the balance of intestinal flora is regulated, and the traditional Chinese medicine composition has multiple efficacies and advantages of antibiosis, antivirus, no drug resistance, greenness, high efficiency and the like.
The fermented traditional Chinese medicine preparation mainly solves the following problems: (1) The application of antibiotics can be reduced, so that the problems of bacterial drug resistance, drug residue and the like are solved; (2) The feed additive has the advantages of benefiting qi, nourishing blood, improving organism immunity and disease resistance, and solving the sub-health problem of sows; (3) improving the productivity of the sow and prolonging the service life of the sow; (4) Can regulate animal intestinal flora balance and further improve organism immunity; (5) Is beneficial to the improvement of the growth quality of piglets and is beneficial to the healthy development of the livestock industry.
Preferably, the lactobacillus plantarum is provided by lactobacillus plantarum bacterial liquid, and the enterococcus faecium is provided by enterococcus faecium bacterial liquid.
The viable count of the X-ray bacterial liquid in the present invention means the viable count of the X-ray bacteria in the X-ray bacterial liquid, for example: the viable count of the lactobacillus plantarum bacterial liquid refers to the viable count of lactobacillus plantarum in the lactobacillus plantarum bacterial liquid.
Preferably, the volume of the lactobacillus plantarum bacterial liquid adopted for each 60-70 g of astragalus is 120-130 mL, and the viable count of the lactobacillus plantarum bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL; the volume of enterococcus faecium bacterial liquid adopted for each 60-70 g of astragalus is 120-130 mL, and the viable count of the enterococcus faecium bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL。
Preferably, in order to ensure the flora balance in the fermentation process and promote the close matching of two lactic acid bacteria, the ratio of the viable count of the lactobacillus plantarum bacterial liquid to the viable count of the enterococcus faecium bacterial liquid is 1:1, volume ratio is 1:1.
further, in the fermented traditional Chinese medicine preparation for the gestation period and/or the lactation period of the sow, the traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 65 parts of astragalus, 45 parts of angelica, 60 parts of radix codonopsis, 60 parts of bighead atractylodes rhizome, 60 parts of poria cocos, 45 parts of prepared rhizome of rehmannia, 35 parts of motherwort, 35 parts of glossy privet fruit, 35 parts of malt, 35 parts of dried orange peel and 35 parts of liquorice; the volume of the lactobacillus plantarum bacterial liquid adopted by each 65g of astragalus is 125mL, and the viable count of the lactobacillus plantarum bacterial liquid is 5.0x10 6 CFU/mL; the volume of enterococcus faecium bacterial liquid adopted for each 65g of astragalus is 125mL, the enterococcus faecium isThe viable count of the bacterial liquid is 5.0X10 6 CFU/mL。
The invention relates to a preparation method of a fermented traditional Chinese medicine preparation for sows in gestation and/or lactation, which adopts the following technical scheme:
a preparation method of any one of the above fermented traditional Chinese medicine preparation comprises the following steps: mixing 60-100 mesh Chinese medicinal composition powder with the lactobacillus, and hermetically fermenting. The preparation method of the fermented traditional Chinese medicine preparation is prepared by mixing traditional Chinese medicines with fermentation strains and then carrying out solid state fermentation, is simple and convenient to operate, has no harmful substances such as industrial three wastes and the like after fermentation, has high utilization rate and good feasibility, is suitable for large-scale industrial production, and has good popularization and application values.
Preferably, the 60-100 mesh traditional Chinese medicine composition powder is obtained by crushing the traditional Chinese medicine composition, sieving the crushed traditional Chinese medicine composition by adopting a 60-100 mesh sieve, and collecting the traditional Chinese medicine composition powder passing through the sieve.
Preferably, the particle size of the traditional Chinese medicine composition powder is 80 meshes.
Preferably, the temperature of the closed fermentation is 25-35 ℃ and the time is 3-7 days. For example, the closed fermentation temperature is 30 ℃, and the time is 5 days.
The lactobacillus in the invention is composed of lactobacillus plantarum and enterococcus faecium. Preferably, the lactobacillus plantarum is provided by lactobacillus plantarum bacterial liquid, and the enterococcus faecium is provided by enterococcus faecium bacterial liquid.
The lactobacillus plantarum bacterial liquid is obtained by activating lactobacillus plantarum bacterial to obtain liquid bacterial, and then diluting the liquid bacterial; the activation of the lactobacillus plantarum species was performed in MRS broth.
The enterococcus faecium bacterial liquid is obtained by activating enterococcus faecium bacterial to obtain liquid bacterial, and then diluting the liquid bacterial; the activation of the enterococcus faecium species is performed in MRS broth.
Preferably, the viable count of the lactobacillus plantarum bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL; the viable count of the enterococcus faecium bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL。
Preferably, the closed fermentation is performed in a thermostatted incubator.
Preferably, when the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows is used, the fermented traditional Chinese medicine preparation is applied to feed for pregnant and/or lactating sows as an additive, so that the weak constitution of the sows in the special period can be aimed at, the sub-health state of the pregnant and lactating sows can be improved, and the immunity and the production performance of the sow in the gestation and/or lactating can be improved.
Preferably, the mass of the fermented traditional Chinese medicine preparation adopted by each ton of feed for sows in gestation and/or lactation is 3000g.
Drawings
FIG. 1 is a diagram showing the body surface of a sow before feeding, as observed in Experimental example 3;
FIG. 2 is a graph showing the body surface of a sow after 15 days of feeding, as observed in Experimental example 3;
FIG. 3 is a graph showing the body surface of a sow after 30 days of feeding, as observed in Experimental example 3;
FIG. 4 shows the results of analysis of intestinal flora diversity of stool samples obtained on days 0, 14 and 28 of the sow in Experimental example 4;
fig. 5 shows the results of the intestinal flora NMDS analysis of the fecal samples from experimental example 4 at day 0, day 14 and day 28.
Detailed Description
The technical effects of the present invention will be described in detail with reference to the following examples.
The raw materials in the following examples and comparative examples are conventional commercial products, wherein the strain number of lactobacillus plantarum is CGMCC 1.557, and the strain number of enterococcus faecium is CGMCC 1.130, and are purchased from China general microbiological culture Collection center; MRS broth medium was purchased from Beijing Obock Biotechnology Co.
Example 1
The traditional Chinese medicine composition for sows in gestation and/or lactation of the embodiment comprises the following traditional Chinese medicine components in parts by weight: 65 parts of astragalus, 45 parts of angelica, 60 parts of radix codonopsis, 60 parts of bighead atractylodes rhizome, 60 parts of poria cocos, 45 parts of prepared rehmannia root, 35 parts of motherwort herb, 35 parts of glossy privet fruit, 35 parts of malt, 35 parts of dried orange peel and 35 parts of liquorice.
Example 2
The traditional Chinese medicine composition for sows in gestation and/or lactation of the embodiment comprises the following traditional Chinese medicine components in parts by weight: 60 parts of astragalus, 40 parts of angelica, 55 parts of codonopsis pilosula, 55 parts of bighead atractylodes rhizome, 55 parts of poria cocos, 40 parts of prepared rehmannia root, 30 parts of motherwort herb, 30 parts of glossy privet fruit, 30 parts of malt, 30 parts of dried orange peel and 30 parts of liquorice.
Example 3
The traditional Chinese medicine composition for sows in gestation and/or lactation of the embodiment comprises the following traditional Chinese medicine components in parts by weight: 70 parts of astragalus, 50 parts of angelica, 65 parts of codonopsis pilosula, 65 parts of bighead atractylodes rhizome, 65 parts of poria cocos, 50 parts of prepared rehmannia root, 40 parts of motherwort herb, 40 parts of glossy privet fruit, 40 parts of malt, 40 parts of dried orange peel and 40 parts of liquorice.
Example 4
The fermented traditional Chinese medicine preparation for sows in gestation and/or lactation of the embodiment is mainly prepared from lactobacillus and the traditional Chinese medicine composition of the embodiment 1, wherein the lactobacillus consists of lactobacillus plantarum and enterococcus faecium.
Example 5
The fermented traditional Chinese medicine preparation for sows in gestation and/or lactation of the embodiment is mainly prepared from lactobacillus and the traditional Chinese medicine composition of the embodiment 2, wherein the lactobacillus consists of lactobacillus plantarum and enterococcus faecium.
Example 6
The fermented traditional Chinese medicine preparation for sows in gestation and/or lactation of the embodiment is mainly prepared from lactobacillus and the traditional Chinese medicine composition of the embodiment 3, wherein the lactobacillus consists of lactobacillus plantarum and enterococcus faecium.
Example 7
The embodiment is a preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows in embodiment 4, comprising the following steps:
1) Activating Lactobacillus plantarum and enterococcus faecium with MRS broth culture medium, and diluting until viable count is 5.0X10 6 CFU/mL to obtain lactobacillus plantarum bacterial liquid and enterococcus faecium bacterial liquid for later use;
2) Crushing the traditional Chinese medicine composition in the embodiment 1, sieving by adopting a sieve with the sieve mesh size of 80 meshes, and collecting traditional Chinese medicine composition powder passing through the sieve;
3) Mixing the lactobacillus plantarum bacterial liquid obtained in the step 1) with the enterococcus faecium bacterial liquid and the traditional Chinese medicine composition powder obtained in the step 2), wherein the dosage of the lactobacillus plantarum bacterial liquid and the enterococcus faecium bacterial liquid is as follows: the volume of lactobacillus plantarum bacterial liquid adopted by each 65g of astragalus is 125mL, the volume of enterococcus faecium bacterial liquid adopted by each 65g of astragalus is 125mL, and then the fermentation is carried out for 5 days at 30 ℃ in a sealing way, thus obtaining the feed.
Example 8
The embodiment is a preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows in embodiment 5, comprising the following steps:
1) Activating Lactobacillus plantarum and enterococcus faecium with MRS broth culture medium, and diluting until viable count is 5.0X10 6 CFU/mL to obtain lactobacillus plantarum bacterial liquid and enterococcus faecium bacterial liquid for later use;
2) Pulverizing the traditional Chinese medicine composition in the embodiment 2, sieving with a sieve with 80 meshes, and collecting traditional Chinese medicine composition powder passing through the sieve;
3) Mixing the lactobacillus plantarum bacterial liquid obtained in the step 1) with the enterococcus faecium bacterial liquid and the traditional Chinese medicine composition powder obtained in the step 2), wherein the dosage of the lactobacillus plantarum bacterial liquid and the enterococcus faecium bacterial liquid is as follows: the volume of lactobacillus plantarum bacterial liquid adopted by each 60g of astragalus is 120mL, the volume of enterococcus faecium bacterial liquid adopted by each 60g of astragalus is 120mL, and then the fermentation is carried out for 7 days at 25 ℃ in a sealing way, thus obtaining the feed.
Example 9
The embodiment is a preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows in embodiment 6, comprising the following steps:
1) Activating Lactobacillus plantarum and enterococcus faecium with MRS broth culture medium, and diluting until viable count is 5.0X10 6 CFU/mL to obtain lactobacillus plantarum bacterial liquid and enterococcus faecium bacterial liquid for later use;
2) Pulverizing the traditional Chinese medicine composition of the example 3, sieving with a sieve with 80 meshes, and collecting traditional Chinese medicine composition powder passing through the sieve;
3) Mixing the lactobacillus plantarum bacterial liquid obtained in the step 1) with the enterococcus faecium bacterial liquid and the traditional Chinese medicine composition powder obtained in the step 2), wherein the dosage of the lactobacillus plantarum bacterial liquid and the enterococcus faecium bacterial liquid is as follows: the volume of lactobacillus plantarum bacterial liquid adopted by each 70g of astragalus is 130mL, the volume of enterococcus faecium bacterial liquid adopted by each 70g of astragalus is 130mL, and then the fermentation is carried out in a sealing way for 3 days at 35 ℃ to obtain the traditional Chinese medicine.
Example 10
The preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows in this embodiment is different from that in embodiment 7 only in that: step 2) the traditional Chinese medicine composition in the embodiment 1 is crushed, then is sieved by adopting a sieve with 60 meshes, and the traditional Chinese medicine composition powder passing through the sieve is collected.
Example 11
The preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows in this embodiment is different from that in embodiment 7 only in that: step 2) the traditional Chinese medicine composition in the embodiment 1 is crushed, and then is sieved by adopting a sieve with the sieve mesh size of 100 meshes, and the traditional Chinese medicine composition powder passing through the sieve is collected.
Comparative example 1
The feed of this comparative example was free of any drugs and additives.
Comparative example 2
The preparation method of the fermented traditional Chinese medicine preparation of the comparative example is different from that of example 7 only in that: the traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 65 parts of astragalus, 45 parts of white peony root, 60 parts of dangshen, 60 parts of bighead atractylodes rhizome, 60 parts of rhizoma alismatis, 45 parts of prepared rehmannia root, 35 parts of motherwort herb, 35 parts of medlar, 35 parts of malt, 35 parts of green tangerine peel and 35 parts of liquorice.
Comparative example 3
The preparation method of the fermented traditional Chinese medicine preparation of the comparative example is different from that of example 7 only in that: the bacillus subtilis is adopted to replace lactobacillus plantarum, and the bacillus licheniformis is adopted to replace enterococcus faecium.
Experimental example 1
After the fermentation of examples 7 to 9 and comparative examples 2 to 3 is completed, taking samples of the fermented traditional Chinese medicine preparation prepared in each example or comparative example, extracting total polysaccharide in the samples by adopting a water extraction and alcohol precipitation method, and measuring the content of the total polysaccharide by using a phenol-sulfuric acid method; simultaneously, extracting organic acid by adopting a distillation method, and detecting the content of formic acid and acetic acid by utilizing a liquid chromatography.
The Chinese medicinal composition of example 1 was pulverized, then sieved with a sieve having a mesh size of 80 mesh, and the powder of the Chinese medicinal composition passing through the sieve was collected, and then the content of total polysaccharide, formic acid and acetic acid in the powder of the Chinese medicinal composition was measured by the method of this experimental example using the powder as a sample. The measurement results of this experimental example are shown in table 1 below.
TABLE 1 Total polysaccharide and organic acid content of examples 1, 7-9 and comparative examples 2-3
Figure BDA0003565433790000101
The results in Table 1 show that compared with the traditional Chinese medicine composition powder without fermentation in example 1, the total polysaccharide and organic acid contents of the fermented traditional Chinese medicine preparations prepared in examples 7-9 are improved to different degrees, wherein the total polysaccharide content after fermentation is improved by 1.87-4.05% compared with the comparative example, which shows that the fermented traditional Chinese medicine preparation is beneficial to improving the immunity of sows; in addition, compared with the fermentation, the formic acid content of the fermented traditional Chinese medicine preparation prepared in the examples 7-9 is increased by 0.0338-0.0501%, and the acetic acid content is increased by 0.0870-0.2389%, which shows that the fermented traditional Chinese medicine preparation is beneficial to regulating the pH of intestinal flora and inhibiting the growth of harmful bacteria.
In contrast, the fermented Chinese medicinal preparation prepared by adopting other Chinese medicinal composition formula in comparative example 2 has lower total polysaccharide content and organic acid content than those of the fermented Chinese medicinal preparations prepared in examples 7-9 of the present application; comparative example 3 after fermentation using bacillus subtilis and bacillus licheniformis, the content of acetic acid was rather reduced.
Experimental example 2
The experimental examples were used for statistical comparison of the related data of the feed prepared in application examples 7 to 11 as additives and the feed of comparative example 1 in terms of productivity of sow.
In 2021, in a core pig farm in the Henan province, sows adopt green ecological breeding, antibiotics are not used, and the problems of long delivery period, a large number of weaning, low survival rate in the lactation period, small weaning weight of piglets and the like are commonly existed. The method comprises the steps of selecting 60 pregnant sows with similar gestation period and gestation time in the same batch (75-80 days), randomly dividing the pregnant sows into 6 groups, 10 groups each, sequentially feeding fermented traditional Chinese medicine preparations prepared in application examples 7-11 as feed additives to groups 1-5, feeding feed containing comparative example 1 to group 6, and controlling the feeding amount and feeding mode of each group to be the same, wherein 3000g of fermented traditional Chinese medicine preparation is added to each ton of feed. The relevant production indexes of sows and piglets were recorded during the continuous use for 60 days, namely, the end of the lactation period (average weaning day age of 25 days in the field), and the production indexes are shown in the following table 2.
Table 2 production performance index of sow and piglet in experimental example 2
Figure BDA0003565433790000111
As can be seen from Table 2, compared with comparative example 1, after the fermented traditional Chinese medicine preparation prepared by the preparation method of the fermented traditional Chinese medicine preparation for pregnant and/or lactating sows of examples 7-11 is taken, the labor of the sows is shortened to 0.6-1.9 h, the litter size is increased by 0.9-2.5, the litter size is increased by 1.18-2.68, the litter size is increased by 0.42-0.69 kg, and the survival rate in the lactating is increased by 2.09% -4.87%. The test result shows that the fermented traditional Chinese medicine preparation has remarkable effect in improving the production performance of sows.
Experimental example 3
The body surfaces of group 1 sows (feed fed with the fermented traditional Chinese medicine preparation prepared in application example 7) in experimental example 2 were observed, wherein the body surfaces of sows before feeding, after feeding for 15 days and after feeding for 30 days are shown in fig. 1 to 3. As can be seen from the comparison of figures 1-3, the body surface of the sow is obviously changed, and the phenomena of rough and messy body surface hairiness, chapped skin and the like of the sow due to the sub-health state are obviously improved, so that the fermented traditional Chinese medicine preparation has excellent effects of improving the immunity of the sow and improving the sub-health state.
Experimental example 4
The feces of the sows of group 1 (fed with the fermented traditional Chinese medicine preparation prepared in application example 7) of experimental example 2 were sampled on days 0, 14 and 28, and the feces samples at three different time points were subjected to intestinal flora diversity analysis by high throughput sequencing, as shown in fig. 4 (note: fig. 4 includes 7 frames, corresponding to seven alpha diversity indexes, respectively, identified in the top gray area thereof: in each frame, the abscissa is a grouping tag, the group a is the result of test day 0, the group B is the result of test day 14, the group C is the result of test day 28, and the ordinate is the value of the corresponding alpha diversity index).
As can be seen from fig. 4, in the fecal sample of the sow fed with the fermented traditional Chinese medicine preparation prepared by the preparation method of the fermented traditional Chinese medicine preparation for gestation and/or lactation sow of application example 7, the Chao1 index and Observeds species index are improved on the 14 th day, and the 28 th day is obviously improved, which indicates that the richness of intestinal flora is gradually and obviously improved after the fermented traditional Chinese medicine preparation is used; the Shannon index and the Simpson index are also improved on the 14 th day, and the 28 th day is obviously improved, which shows that the diversity of intestinal colonies is gradually and obviously improved after the fermented traditional Chinese medicine preparation is used; in addition, the Pielou's evenness index shows the same change trend, which shows that the distribution uniformity of intestinal flora is better after the fermented traditional Chinese medicine preparation is used.
Meanwhile, intestinal flora NMDS analysis was performed on stool samples of sows on day 0, day 14 and day 28 in the experimental example 2 group 1, and the results are shown in FIG. 5 (note: each dot represents one sample in FIG. 5, and different colored dots indicate different samples (groups). Since NMDS adopts rank ordering, the closer (farther) the distance between the two dots can be approximately considered, indicating that the smaller (larger) the difference of microbial communities in the two samples. The results in fig. 5 show that: by using the fermented traditional Chinese medicine preparation, the differences of microbial communities in intestinal tracts of different individuals are gradually reduced on the 14 th day except for samples with large individual differences, and the differences of microbial communities in intestinal tracts of different individuals are further reduced (the differences are obvious) on the 28 th day, so that the intestinal flora can be gradually consistent by using the fermented traditional Chinese medicine preparation.
In addition, stool samples of sows on day 0, day 14 and day 28 of experimental example 2 group 1 were sequenced, and the population composition of the intestinal flora of sows was analyzed at the phylum and genus classification level, and the results are shown in tables 3 and 4, respectively.
TABLE 3 sow intestinal flora portal level analysis (%)
Project Day 0 14 days For 28 days
Thick wall fungus door 89.77±2.90a 89.76±4.43a 80.96±4.83b
Bacteroides door 11.23±1.04a 7.43±1.08b 11.08±0.98a
Thick-walled bacteria/bacteroides 11.62±1.53a 8.06±0.91b 7.36±0.76b
Note that: the data in Table 3 are expressed in terms of mean.+ -. Standard deviation, the letters a and b are shoulder marks, and indicate whether there is a significant difference between the data, the letters are the same and represent the difference is not significant (P.gtoreq.0.05), and the shoulder marks are different and represent the difference is significant (P.gtoreq.0.05).
As shown in Table 3, more than 90% of the intestinal flora of the sow are distributed in the phylum of the firmicutes and the bacteroides, and after the fermented traditional Chinese medicine preparation is added, the relative abundance of the firmicutes is obviously reduced (P is less than or equal to 0.05) at day 28; the relative abundance of the bacteroides is obviously reduced (P is less than or equal to 0.05) on the 14 th day, and the bacteroides is restored to the level before use on the 28 th day; the ratio of firmicutes/bacteroides was significantly reduced (P.ltoreq.0.05) on day 14, continuing until day 28. The results show that the fermented traditional Chinese medicine preparation can improve the structure of intestinal flora, reduce the relative abundance of the firmicutes in the intestinal flora of sows, and simultaneously reduce the ratio of the firmicutes to the bacteroides. The fermented traditional Chinese medicine preparation disclosed by the invention can promote the nutrient absorption of sows to a certain extent, prevent the metabolic diseases of sows and the incidence rate of pathological obesity such as fatty liver and the like, has an important effect on adjusting the body condition (such as backfat thickness) of sows, and has an important guiding significance in production.
TABLE 4 sow intestinal flora level analysis (%)
Project Day 0 14 days For 28 days
SMB53 28.10±2.29a 20.42±2.55b 16.25±2.85c
Lactobacillus 2.91±0.33c 12.83±1.40b 18.96±1.15a
Oscillospira 2.35±0.29c 2.11±0.20b 1.82±0.17a
Note that: the data in Table 4 are expressed in terms of mean.+ -. Standard deviation, and the letters a, b, c are shoulder marks, indicating whether there is a significant difference between the data, the same letters represent the difference not significant (P.gtoreq.0.05), and the different shoulder marks represent the difference significant (P.gtoreq.0.05).
As shown in Table 4, the fermented traditional Chinese medicine preparation provided by the invention can obviously reduce the proportion of SMB53 (SMB 53 genus) and Oscillospira (Oscillus) and obviously improve the abundance of Lactobacillus. The fermented traditional Chinese medicine preparation disclosed by the invention is added into sow daily ration, so that the abundance of beneficial intestinal bacteria can be improved, and the abundance of partial harmful bacteria can be reduced.
The experimental examples show that after the traditional Chinese medicine fermentation preparation disclosed by the invention is applied as a feed additive for sows in gestation and/or lactation, the sub-health state of the sows can be comprehensively improved, and the health degree of the sows is improved, so that the overall production performance of the sows is improved, and meanwhile, the traditional Chinese medicine fermentation preparation has a remarkable effect on regulating the microecological balance of intestinal flora of the sows.

Claims (5)

1. The fermented traditional Chinese medicine composition for the gestation period and/or the lactation period of the sows is characterized by being prepared by fermenting lactobacillus and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 60-70 parts of astragalus membranaceus, 40-50 parts of angelica sinensis, 55-65 parts of codonopsis pilosula, 55-65 parts of bighead atractylodes rhizome, 55-65 parts of poria cocos, 40-50 parts of prepared rehmannia root, 30-40 parts of motherwort herb, 30-40 parts of glossy privet fruit, 30-40 parts of malt, 30-40 parts of dried orange peel and 30-40 parts of liquorice;
the lactobacillus consists of lactobacillus plantarum and enterococcus faecium; the volume of the lactobacillus plantarum bacterial liquid adopted by each 60-70 g of astragalus is 120-130 mL, and the viable count of the lactobacillus plantarum bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL; the volume of enterococcus faecium bacterial liquid adopted for each 60-70 g of astragalus is 120-130 mL, and the viable count of the enterococcus faecium bacterial liquid is (1.0-10.0) multiplied by 10 6 CFU/mL; the ratio of the viable count of the lactobacillus plantarum bacterial liquid to the viable count of the enterococcus faecium bacterial liquid is 1:1, volume ratio is 1:1.
2. the fermented traditional Chinese medicine composition for sow gestation and/or lactation according to claim 1, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 65 parts of astragalus, 45 parts of angelica, 60 parts of radix codonopsis, 60 parts of bighead atractylodes rhizome, 60 parts of poria cocos, 45 parts of prepared rhizome of rehmannia, 35 parts of motherwort, 35 parts of glossy privet fruit, 35 parts of malt, 35 parts of dried orange peel and 35 parts of liquorice; the volume of the lactobacillus plantarum bacterial liquid adopted by each 65g of astragalus is 125mL, and the viable count of the lactobacillus plantarum bacterial liquid is 5.0x10 6 CFU/mL; the volume of enterococcus faecium bacterial liquid adopted for each 65g of astragalus is 125mL, and the viable count of the enterococcus faecium bacterial liquid is 5.0X10 6 CFU/mL。
3. A method for preparing a fermented traditional Chinese medicine composition for sows in gestation and/or lactation according to any one of claims 1-2, which is characterized by comprising the following steps: mixing 60-100 mesh traditional Chinese medicine composition powder with the lactobacillus, and hermetically fermenting to obtain the traditional Chinese medicine composition.
4. The method for preparing a fermented Chinese medicinal composition for gestation and/or lactation of sow according to claim 3, wherein the particle size of the powder of the Chinese medicinal composition is 80 mesh.
5. The method for preparing the fermented traditional Chinese medicine composition for the gestation and/or lactation of sows as claimed in claim 3, wherein the temperature of the closed fermentation is 25-35 ℃ and the time is 3-7 days.
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