CN109350655B - Fermented traditional Chinese medicine for improving growth and reproductive performance of pigs and preparation method thereof - Google Patents
Fermented traditional Chinese medicine for improving growth and reproductive performance of pigs and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a fermented traditional Chinese medicine for improving the growth and reproductive performance of pigs, which is prepared by fermenting astragalus, codonopsis pilosula, bighead atractylodes rhizome, white paeony root, Chinese angelica, liquorice, poria cocos and acanthopanax senticosus through specific probiotics. The fermented traditional Chinese medicine can obviously improve the health condition of pigs and improve the growth and reproductive performance of the pigs. The preparation method of the fermented traditional Chinese medicine comprises inoculating strains to the traditional Chinese medicine components, and performing solid state fermentation, and has the advantages of simple process and easy industrial production.
Description
Technical Field
The invention relates to the technical field of traditional Chinese veterinary medicine preparations, in particular to a fermented traditional Chinese medicine for improving growth and reproductive performance of pigs and a preparation method thereof.
Background
Antibiotics play an important role in preventing and treating animal epidemic diseases, improving breeding benefits and guaranteeing public health safety, but the problems of antibiotic residues, bacterial drug resistance and the like appearing in recent years bring hidden dangers to food safety and public health safety. Therefore, the development of safe, effective and economical antibiotic substitutes becomes a hotspot of current cultivation research.
The traditional Chinese medicine has rich resources, can be widely applied to various cultured animals, has the characteristics of natural sources, functional diversity, safety, reliability, economy, environmental friendliness and the like, and can improve the resistance of the cultured animals, improve the production performance and improve the quality of livestock and poultry products. However, most of the traditional Chinese medicines are directly added after being crushed at present, and have the defects of large addition amount, slow effect and the like.
At present, the research of applying microbial fermentation to the processing technology of traditional Chinese medicines is available, however, both the basic theory aspect and the research means are still in the initial stage, particularly, the research on compound fermentation is relatively less, and the number of new fermentation products produced in batch is small. The reason is that the modern fermentation process faces some critical problems, such as ambiguity of the traditional Chinese medicine self system, complexity of contained components, incomplete research of fermentation mechanism, diversity of microorganism growth characteristics, uncertainty of biotransformation effect generated in the traditional Chinese medicine fermentation process, and the like. Therefore, modern fermentation technology needs to be further improved, and further research and improvement are needed in aspects such as breeding and purification of strains, research and application of various microbial fermentation processes, confirmation of fermentation mechanisms, how to screen traditional Chinese medicine fermentation, establishment of a multi-dimensional evaluation system and the like.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a fermented traditional Chinese medicine capable of improving the growth and reproductive performance of pigs, which has the advantages of no medicine residue, safety, reliability, and clear and stable effect. The invention also provides a preparation method of the fermented traditional Chinese medicine.
In order to achieve the purpose, the invention adopts the following technical scheme:
a fermented traditional Chinese medicine for improving pig growth and reproductive performance is prepared by fermenting traditional Chinese medicine raw materials with probiotics, wherein the traditional Chinese medicine raw materials comprise the following components in parts by mass: 5-15% of astragalus, 10-30% of codonopsis pilosula, 10-30% of bighead atractylodes rhizome, 10-25% of white peony root, 5-20% of angelica, 10-30% of liquorice, 10-25% of poria cocos and 5-15% of acanthopanax; the probiotics are bacillus subtilis and lactobacillus acidophilus.
Preferably, in order to enhance the effect of the product, part of the Chinese medicinal materials may be processed products, such as radix astragali Preparata, radix Codonopsis Preparata, Atractylodis rhizoma Preparata, radix Paeoniae alba Preparata, Glycyrrhrizae radix Preparata, and radix Angelicae sinensis Preparata.
Preferably, the traditional Chinese medicine raw materials comprise the following components in percentage by mass: 5-10% of astragalus membranaceus, 12-15% of codonopsis pilosula, 10-15% of bighead atractylodes rhizome, 10-15% of white peony root, 12-15% of angelica sinensis, 10-15% of liquorice, 10-15% of poria cocos and 5-8% of acanthopanax.
Further preferably, the traditional Chinese medicine raw materials comprise the following components in percentage by mass: 7.1% of astragalus, 14.3% of codonopsis pilosula, 14.3% of bighead atractylodes rhizome, 14.3% of white paeony root, 14.3% of angelica, 14.3% of liquorice, 14.3% of poria cocos and 7.1% of acanthopanax.
Preferably, the content of each probiotic is as follows: bacillus subtilis greater than or equal to 1.0 × 107cfu/g, Lactobacillus acidophilus is more than or equal to 1.0 multiplied by 107cfu/g。
The traditional Chinese medicine raw materials used by the invention have the following effects:
astragalus root has the functions of enhancing organism immunity, protecting liver, resisting aging and stress, and has better effect of invigorating qi and invigorating yang after being roasted with honey.
Codonopsis pilosula, radix Codonopsis acts to strengthen the middle-jiao and replenish qi. It is not dry but greasy, and is good at invigorating spleen, invigorating qi, and nourishing blood. Contains saponin and trace alkaloids, and can enhance immunity; can dilate peripheral blood vessel, improve microcirculation, and enhance hemopoietic function. The efficacy of tonifying middle-jiao and Qi after being stir-baked with honey is enhanced.
Bai Zhu strengthens spleen and qi, eliminates dampness and strengthens superficies. Largehead atractylodes rhizome stir-baked with bran can alleviate dryness and strengthen the functions of strengthening spleen and stomach.
Radix Paeoniae alba has effects of suppressing hyperactive liver, relieving pain, nourishing blood, astringing yin, and softening liver, astringing yin, and relieving pain after vinegar processing.
Dang Gui can tonify blood and activate blood, moisten intestines to relieve constipation. It is a key herb for leukemia, and it is combined with Huang Qi to nourish the blood of heart and liver, nourish blood and harmonize nutrient. After stir-baked with earth, the effects of enriching blood and relaxing and lubricating are enhanced.
Licorice root, radix Glycyrrhizae can tonify spleen and qi, clear heat and remove toxicity. Has acid and spasm resisting, antiinflammatory, and antiallergic effects, and can be used for treating ulcer; contains glycyrrhizin, and has effects in removing toxic substances and relieving various poisoning and bacterial endotoxin. Honey-baked licorice root, radix Glycyrrhizae Praeparata, is sweet and warm and can tonify spleen, nourish both qi and blood.
Poria cocos, being mild in nature, can eliminate pathogens, strengthen body resistance, excrete dampness and induce diuresis, reduce swelling, tonify spleen and calm heart.
Acanthopanax senticosus has the effects of tonifying liver and kidney, strengthening spleen and stomach and tonifying lung qi.
According to the invention, through screening the traditional Chinese medicine raw materials, the traditional Chinese medicines with the effects of tonifying qi and blood, strengthening liver and kidney, strengthening spleen and stomach and strengthening defensive exterior are selected for combination, and then are fermented by bacillus subtilis and lactobacillus acidophilus, so that substances which are not easy to absorb in the traditional Chinese medicines are fully degraded, the absorption rate and the efficacy of the traditional Chinese medicines are improved, the toxic and side effects of the traditional Chinese medicines are reduced, the palatability of the traditional Chinese medicines is improved, and meanwhile, the function of conditioning intestinal tracts by using the microecological.
The selected strains act in a synergistic manner, so that the cellulose in the tissues of the traditional Chinese medicine has excellent degradation capability, the effective components of the traditional Chinese medicine can be released to the maximum extent, and the drug effect of the traditional Chinese medicine is obviously improved.
The invention also provides a preparation method of the fermented traditional Chinese medicine, which at least comprises the following steps: crushing the traditional Chinese medicine raw materials, weighing the raw materials in parts by weight, mixing and sterilizing; then inoculating the bacillus subtilis and the lactobacillus acidophilus, and fermenting and culturing for 5-10 days at the temperature of 30-40 ℃ to obtain the bacillus subtilis and lactobacillus acidophilus fermented lactobacillus.
Preferably, the ratio of the number of bacteria inoculated with the bacillus subtilis to the number inoculated with the lactobacillus acidophilus is 1: 1.
The optimized inoculation amount of each strain can ensure that the strains do not generate mutual inhibition in the fermentation process, so that the fermentation process is effectively carried out, the fermentation effect and the viable count in the final product are ensured, and the drug effect of the product is stable and reliable.
Preferably, the Chinese medicinal raw materials are crushed and sieved by a 60-120 mesh sieve.
Preferably, the sterilization is performed at 100 ℃ for 30-60 min.
Preferably, the fermentation is solid state fermentation, the traditional Chinese medicine inoculated with the strain can be placed in a fermentation bag, a one-way air valve is arranged on the fermentation bag, gas generated by fermentation can be released from the fermentation bag, and outside air cannot enter the fermentation bag, so that the effective performance of the fermentation process is ensured.
Detailed Description
In order to better explain the technical scheme of the invention, the following examples are further exemplified.
Example 1
Pulverizing the above Chinese medicinal materials, sieving with 80 mesh sieve, weighing honey-fried radix astragali 7.1kg, honey-fried radix Codonopsis 14.3kg, parched Atractylodis rhizoma 14.3kg, vinegar-fried radix Paeoniae alba 14.3kg, soil-fried radix Angelicae sinensis 14.3kg, honey-fried Glycyrrhrizae radix 14.3kg, Poria 14.3kg, and radix Acanthopanacis Senticosi 7.1kg, mixing, sterilizing at 100 deg.C for 40min, inoculating Bacillus subtilis fermentation broth (diluted to 5 × 10 with sterile water)7cfu/mL)15L, Lactobacillus acidophilus fermentation broth (diluted to 5 × 10 with sterile water7cfu/mL)15L, fully and uniformly mixed, put into a fermentation bag with a one-way valve, and fermented and cultured for 7d at 37 ℃.
Test example 1 Effect on health of sow, reproductive Property and growth Performance of farrowing pig
(1) Test animals and groups
72 sows are all multiparous sows, the number of births is 2-4, the weight is basically close, and the body condition is healthy. After numbering uniformly, the test group was divided into a control group and a test group, which had 20 and 52 heads, respectively. The control group was fed with the basic ration, and the test group additionally added 1kg of the fermented traditional Chinese medicine prepared in example 1 per ton of the basic ration. The test period is from breeding to weaning of the born piglets. The daily management of the test group and the control group is carried out according to the conventional management of a pig farm.
(2) Index and method of measurement
The number of the sick head and the abortion condition are recorded by taking a group as a unit, the birth process of each sow is recorded, and indexes such as litter size, number of born alive litter, number of weak dead teratocarcinoma, weight of newborn litter, number of weaned piglets (21 days old), weight of weaned litter (21 days old) and the like are recorded by taking a litter as a unit. Calculating the runoff rate, the average birth process, the average litter size, the weak and dead teratogenesis rate, the average live litter size, the average birth weight, the average weaning weight, the average daily gain and the weaning survival rate. Wherein the abortion rate is the number of the aborted sows/the group number multiplied by 100 percent; weak and dead teratogenesis rate is the group (weak fetus + dead fetus + malformed fetus)/total number born of the group is multiplied by 100 percent; the survival rate is equal to the number born alive of the group/the number born of the group multiplied by 100 percent; average daily gain (average weaning weight-average primary weight)/21; the weaning survival rate is the total number of weaned piglets per group/the total number of live piglets per group multiplied by 100%.
(3) Results
The influence of fermented traditional Chinese medicines on the health condition of the sows is as follows: the incidence of the test group was reduced 61.67% compared to the control group.
② the influence of the fermented traditional Chinese medicine on the reproductive performance of the sows: compared with the control group, the test group has the advantages of reducing the abortion rate, obviously shortening the sow birth process (p is less than 0.01), improving the average litter size (p is less than 0.05), improving the average live litter size (p is less than 0.01), increasing the litter weight of born piglets (p is less than 0.01), increasing the average litter weight (p is less than 0.05), and reducing the weak death teratogenesis rate (reducing 36.73%).
| Item | Control group | Test group |
| Number of sow heads (head) | 20 | 52 |
| Flow rate (%) | 5.00 | 1.92 |
| Mean labor (min) | 225.26±37.17 | 200.39±25.53** |
| Average litter size (head) | 10.11±1.15 | 10.78±1.10* |
| Weak death teratogenesis rate (%) | 8.33 | 5.27 |
| Average alive number (head) | 9.68±1.20 | 10.49±1.01** |
| Weight of newborn litter (kg) | 16.02±1.73 | 17.60±1.50** |
| Average initial weight (kg) | 1.59±0.07 | 1.64±0.08* |
Remarking: "+" indicates significant difference (p < 0.05) compared to control group, "+" indicates very significant difference (p < 0.01), and no notation indicates insignificant difference (p > 0.05).
③ the influence of the fermented traditional Chinese medicine on the growth performance of the piglets born by the sows: compared with a control group, the test group can obviously improve the number of weaned piglets born by the sow (p is less than 0.01), improve the weaning survival rate (1.95%), increase the weight of weaned litter (p is less than 0.01) and increase the average weaning weight (p is less than 0.05); compared with the control group, the average daily gain of the test group is slightly increased, but the difference is not significant (p is more than 0.05).
| Item | Control group | Test group |
| Number of weaned piglets | 8.84±0.83 | 9.76±1.01** |
| Survival rate of weaning (%) | 91.30 | 93.08 |
| Weight of weaning litter (kg) | 57.02±5.06 | 64.21±6.22** |
| Average weight weaned (kg) | 6.45±0.18 | 6.58±0.19* |
| Average daily gain (kg) | 0.232±0.01 | 0.236±0.01 |
Remarking: "+" indicates significant difference (p < 0.05) compared to control group, "+" indicates very significant difference (p < 0.01), and no notation indicates insignificant difference (p > 0.05).
The test results show that the fermented traditional Chinese medicine can obviously reduce the sow flow rate, shorten the production process (p is less than 0.01), improve the average litter size (p is less than 0.05), improve the average live number (p is less than 0.01), increase the litter weight of born piglets (p is less than 0.01), increase the average litter weight (p is less than 0.05), reduce the weak death rate (36.73%), improve the number of weaned piglets (p is less than 0.01), improve the weaning survival rate (1.95%), increase the litter weight of weaned piglets (p is less than 0.01) and increase the average weaning value (p is less than 0.05).
Test example 2 Effect on growth Performance of commercial pigs
(1) Test animals and groups
50 big-and-long ternary hybrid pigs of 28-day-old weaning pigs and with close body weight, half of the male and female pigs, are randomly divided into 2 groups, 20 control groups and 30 test groups. The test period is from nursing to slaughtering, and the test comprises 3 stages of nursing period, growth period and fattening period, wherein the nursing period is 30d, the growth period is 40d and the fattening period is 60 d. The daily management of the test group and the control group is carried out according to the conventional management of a pig farm.
(2) Index and method of measurement
Weighing the pigs one by one in 28 days, 58 days, 98 days and 158 days in the morning on an empty stomach, and recording the feed intake, the health condition of the pigs, the death and elutriation condition and the like of each group every day. And calculating the average daily gain, the average daily feed intake and the material-weight ratio of each group in each test period.
(3) Test results
The influence of the fermented traditional Chinese medicine on the growth performance of nursery pigs is as follows: during the test period, the control group and the test group do not die. Compared with the control group, the initial weight difference of the test group at 28 days of age is not significant (p is more than 0.05), the weight of the test group at 58 days of age is obviously increased (p is less than 0.01), the average daily gain is obviously increased (p is less than 0.01), the average daily feed intake is slightly improved, and the feed-weight ratio is obviously reduced (reduced by 5.38%).
| Item | Control group | Test group |
| Number of heads (head) | 20 | 30 |
| Death rate (%) | 0 | 0 |
| 28 day old body weight (kg) | 6.98±0.18 | 6.95±0.19 |
| Body weight (kg) at age 58 days | 18.19±0.55 | 18.83±0.36** |
| Average daily gain (kg) | 0.374±0.020 | 0.396±0.008** |
| Average daily food intake (kg) | 0.697 | 0.698 |
| Material to weight ratio | 1.86 | 1.76 |
Remarking: "x" indicates very significant difference (p < 0.01) compared to the control group, and no notation indicates insignificant difference (p > 0.05).
② the influence of the fermented traditional Chinese medicine on the growth performance of the growing pig: during the test period, the control group and the test group both die 1 pig, and the death and culling rates are 5.00% and 3.33% respectively. Compared with the control group, the weight of the test group at 98 days of age is obviously increased (p is less than 0.01), the average daily gain is obviously increased (p is less than 0.01), the average daily feed intake is close, and the feed-weight ratio is obviously reduced (reduced by 4.67%).
| Item | Control group | Test group |
| Number of heads (head) | 19 | 29 |
| Death rate (%) | 5.00 | 3.33 |
| Body weight (kg) at age 58 days | 18.19±0.55 | 18.83±0.36** |
| 98 days old body weight (kg) | 43.25±1.00 | 44.98±0.82** |
| Average daily gain (kg) | 0.627±0.026 | 0.654±0.019** |
| Average daily food intake (kg) | 1.61 | 1.61 |
| Material to weight ratio | 2.57 | 2.45 |
Remarking: ". indicates that the difference was very significant compared to the control group (p < 0.01).
③ the influence of the fermented traditional Chinese medicine on the growth performance of the fattening pigs: during the test period, no death phenomenon occurs in the fattening pigs of the control group and the test group. Compared with the control group, the weight of the test group at the age of 158 days is obviously increased (p is less than 0.01), the average daily gain is obviously increased (p is less than 0.01), the average daily feed intake is slightly improved, and the feed-weight ratio is obviously reduced (reduced by 5.44%).
| Item | Control group | Test group |
| Number of heads (head) | 19 | 29 |
| Death rate (%) | 0 | 0 |
| 98 days old body weight (kg) | 43.25±1.00 | 44.98±0.82** |
| 158 days old body weight (kg) | 94.40±2.43 | 101.41±2.85** |
| Average daily gain (kg) | 0.852±0.043 | 0.940±0.041** |
| Average daily food intake (kg) | 2.51 | 2.62 |
| Material to weight ratio | 2.94 | 2.78 |
Remarking: ". indicates that the difference was very significant compared to the control group (p < 0.01).
Fourthly, the influence of the fermented traditional Chinese medicines on the growth performance of the commercial pigs in the whole test period is as follows: during the test period, the control group and the test group both died 1, and the death and culling rates were 5.00% and 3.33%, respectively. Compared with a control group, the weight of the test group at the age of 158 days is obviously increased (p is less than 0.01), the average daily gain is obviously increased (p is less than 0.05), the average daily feed intake is obviously improved (improved by 1.85 percent), and the material-to-weight ratio is obviously reduced (reduced by 4.49 percent).
| Item | Control group | Test group |
| Number of heads (head) | 20 | 30 |
| Death rate (%) | 5.00 | 3.33 |
| 28 day old body weight (kg) | 6.98±0.18 | 6.95±0.19 |
| 158 days old body weight (kg) | 94.40±2.43 | 101.41±2.85** |
| Average daily gain (kg) | 0.649±0.109 | 0.710±0.096* |
| Average daily food intake (kg) | 1.729 | 1.807 |
| Material to weight ratio | 2.67 | 2.55 |
Remarking: "+" indicates significant difference (p < 0.05) compared to control group, "+" indicates very significant difference (p < 0.01), and no notation indicates insignificant difference (p > 0.05).
The test results show that the fermented traditional Chinese medicine can obviously increase the weight (p is less than 0.01) and the average daily gain (p is less than 0.05 or p is less than 0.01) of the nursery pigs, the growing pigs, the fattening pigs and the commercial pigs in the whole test period, reduce the feed-weight ratio (the weight of the nursery pigs, the growing pigs, the fattening pigs and the commercial pigs in the whole test period is respectively reduced by 5.38%, 4.67%, 5.44% and 4.49%), improve the growth performance of the commercial pigs and increase the breeding benefit.
In order to better illustrate the technical solution of the present invention, further comparison is made below by means of a comparative example and an example of the present invention.
Comparative example 1
The bacillus subtilis in the example 1 is replaced by bacillus licheniformis, and the other components and the preparation method are the same as the example 1, so that the fermented traditional Chinese medicine reference 1 is obtained.
Comparative example 2
The lactobacillus acidophilus in example 1 was replaced with lactobacillus plantarum, and the remaining components and preparation methods were the same as in example 1, to obtain fermented traditional Chinese medicine control 2.
The reference substance 1 and the reference substance 2 are added into basal ration of sows and commercial pigs according to the same mixing amount of the fermented traditional Chinese medicines in the example 1, the influence of the reference substance on the growth performance of the commercial pigs is equivalent to that of the product in the example 1, and the reference substance has no obvious difference on the birth process of the sows, the litter weight of birth and the average birth weight compared with the control group fed with the basal ration.
Comparative example 3
The caulis spatholobi in the example 1 is used for replacing angelica, and the rest components and the preparation method are the same as the example 1, so that a fermented traditional Chinese medicine reference substance 3 is obtained.
Comparative example 4
The coix seed in the example 1 replaces the tuckahoe, and the other components and the preparation method are the same as the example 1, so as to obtain the fermented traditional Chinese medicine reference substance 4.
The reference substance 3 and the reference substance 4 are added into basal ration of sows and commercial pigs according to the same mixing amount of the fermented traditional Chinese medicines in the embodiment 1, the influence of the reference substance on the feed-weight ratio of the commercial pigs in the whole test period is 2-5% higher than that of the product in the embodiment 1, and the reproductive performance of the sows is not obviously different from that of a control group fed with the basal ration.
Examples 2 to 6
The compositions of the Chinese herbs of examples 2-6 are as follows (unit kg):
| components | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 |
| Astragalus membranaceus | 5 | 15 | 10 | 6 | 5 |
| Codonopsis pilosula | 30 | 10 | 10 | 15 | 10 |
| White atractylodes rhizome | 10 | 15 | 10 | 11 | 30 |
| White peony root | 25 | 10 | 10 | 15 | 10 |
| Radix Angelicae sinensis | 5 | 5 | 15 | 10 | 20 |
| Licorice root, radix Glycyrrhizae | 10 | 10 | 30 | 18 | 10 |
| Poria cocos (Schw.) wolf | 10 | 25 | 10 | 10 | 10 |
| Radix Et caulis Acanthopanacis Senticosi | 5 | 10 | 5 | 15 | 5 |
Examples 2-6 were prepared in the same manner as in example 1.
The fermented traditional Chinese medicines in the examples 2 to 6 have the reproductive performance of sows and the growth performance of commercial pigs which are obviously different from those of the control group, but have slightly poorer effects than those of the example 1.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (6)
1. The application of fermented traditional Chinese medicine in preparing the medicine for improving the reproductive performance of pigs is characterized in that the fermented traditional Chinese medicine is prepared by fermenting the following traditional Chinese medicine raw materials by probiotics: 7.1% of astragalus, 14.3% of codonopsis pilosula, 14.3% of bighead atractylodes rhizome, 14.3% of white paeony root, 14.3% of angelica, 14.3% of liquorice, 14.3% of poria cocos and 7.1% of acanthopanax; the probiotics are bacillus subtilis and lactobacillus acidophilus; the preparation method of the fermented traditional Chinese medicine at least comprises the following steps: crushing the traditional Chinese medicine raw materials, weighing the raw materials in parts by weight, mixing and sterilizing; then inoculating the bacillus subtilis and the lactobacillus acidophilus, and fermenting and culturing for 5-10 days at the temperature of 30-40 ℃ to obtain the bacillus subtilis and lactobacillus acidophilus fermented lactobacillus.
2. The use according to claim 1, wherein the probiotic bacteria are present in an amount of: bacillus subtilis greater than or equal to 1.0 × 107cfu/g, Lactobacillus acidophilus is more than or equal to 1.0 multiplied by 107cfu/g。
3. The use of claim 1, wherein the ratio of bacteria inoculated with said bacillus subtilis to lactobacillus acidophilus is 1: 1.
4. The use of claim 1, wherein: the Chinese medicinal raw materials are crushed and sieved by a 60-120 mesh sieve.
5. The use of claim 1, wherein: the sterilization is performed at 100 ℃ for 30-60 min.
6. The use of claim 1, wherein: the fermentation is solid state fermentation.
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| CN110859897A (en) * | 2019-12-03 | 2020-03-06 | 六安恒佳生物科技有限公司 | Fermented traditional Chinese medicine additive for preventing postweaning multisystemic wasting syndrome |
| CN111388613B (en) * | 2020-05-19 | 2021-11-23 | 山西中医药大学 | Traditional Chinese veterinary medicine astragalus root compound particles for promoting growth and enhancing immunity |
| CN112741869A (en) * | 2021-03-18 | 2021-05-04 | 河南省帝一方生物制药有限公司 | Fermented traditional Chinese medicine preparation for strengthening body resistance and eliminating pathogenic factors of livestock and poultry and preparation method thereof |
| CN113973999B (en) * | 2021-10-27 | 2023-07-07 | 江西农业大学 | Fermented compound traditional Chinese medicine additive for improving water content and PSY of sows and sheep and preparation method thereof |
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Denomination of invention: A fermented traditional Chinese medicine and its preparation method for improving pig growth and reproductive performance Effective date of registration: 20230619 Granted publication date: 20210622 Pledgee: Cangzhou Bank Co.,Ltd. Shijiazhuang Branch Pledgor: HEBEI JUNYU PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980044676 |