CN102367416A - Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive - Google Patents
Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive Download PDFInfo
- Publication number
- CN102367416A CN102367416A CN2011102768854A CN201110276885A CN102367416A CN 102367416 A CN102367416 A CN 102367416A CN 2011102768854 A CN2011102768854 A CN 2011102768854A CN 201110276885 A CN201110276885 A CN 201110276885A CN 102367416 A CN102367416 A CN 102367416A
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- feed
- traditional chinese
- probiotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 63
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 63
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 61
- 239000003814 drug Substances 0.000 title claims abstract description 58
- 239000007787 solid Substances 0.000 title claims abstract description 29
- 239000003674 animal food additive Substances 0.000 title abstract description 10
- 239000001963 growth medium Substances 0.000 title abstract description 5
- 238000012136 culture method Methods 0.000 title abstract 2
- 241000283690 Bos taurus Species 0.000 claims abstract description 41
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 27
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 13
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 11
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 11
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 11
- 239000012138 yeast extract Substances 0.000 claims abstract description 11
- 240000008042 Zea mays Species 0.000 claims abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 2
- 235000005822 corn Nutrition 0.000 claims abstract 2
- 239000000203 mixture Substances 0.000 claims description 34
- 241000894006 Bacteria Species 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 22
- 210000000582 semen Anatomy 0.000 claims description 20
- 241000050051 Chelone glabra Species 0.000 claims description 12
- 241000245665 Taraxacum Species 0.000 claims description 12
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 claims description 11
- 241000756943 Codonopsis Species 0.000 claims description 11
- 240000007890 Leonurus cardiaca Species 0.000 claims description 11
- 235000000802 Leonurus cardiaca ssp. villosus Nutrition 0.000 claims description 11
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 10
- 239000009636 Huang Qi Substances 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000010563 solid-state fermentation Methods 0.000 claims 3
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 235000012054 meals Nutrition 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 208000004396 mastitis Diseases 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 230000009471 action Effects 0.000 abstract description 4
- 230000000144 pharmacologic effect Effects 0.000 abstract description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 2
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 abstract 1
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 229940095100 fulvic acid Drugs 0.000 abstract 1
- 239000002509 fulvic acid Substances 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 description 28
- 239000008267 milk Substances 0.000 description 28
- 210000004080 milk Anatomy 0.000 description 28
- 239000002609 medium Substances 0.000 description 19
- 239000000654 additive Substances 0.000 description 10
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- 230000017531 blood circulation Effects 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 229940126678 chinese medicines Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 241001061264 Astragalus Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 2
- 241000721047 Danaus plexippus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 2
- 235000011941 Tilia x europaea Nutrition 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 235000006533 astragalus Nutrition 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000000920 calcium hydroxide Substances 0.000 description 2
- 235000011116 calcium hydroxide Nutrition 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000004571 lime Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 210000004233 talus Anatomy 0.000 description 2
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000202726 Bupleurum Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 240000004670 Glycyrrhiza echinata Species 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- 239000004378 Glycyrrhizin Substances 0.000 description 1
- 240000002045 Guettarda speciosa Species 0.000 description 1
- 235000001287 Guettarda speciosa Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 240000005384 Rhizopus oryzae Species 0.000 description 1
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 241001530119 Vaccaria Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 238000001354 calcination Methods 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 230000008975 immunomodulatory function Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000010341 ping gan Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
- 150000008130 triterpenoid saponins Chemical class 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a traditional Chinese medicine probiotic solid culture medium for cow feed, a culture method and a feed additive. The traditional Chinese medicine probiotic solid culture medium for cow feed comprises the following components in parts by mass: 60-70 parts of wheat bran, 15-20 parts of bean pulp, 10-15 parts of corn flour, 2-3 parts of brown sugar, 2-3 parts of traditional Chinese medicine component, 2-4 parts of light calcium carbonate, 0.5-0.8 part of yeast extract and 0.2-0.5 part of fulvic acid. By implementing the embodiment of the invention, the activity of active probiotic strains and the colonization performance in the intestinal tract can be greatly improved; and the feed additive prepared from the traditional Chinese medicine probiotic solid culture medium is rich in traditional Chinese medicine components, and active components having pharmacological action in the traditional Chinese medicine components can be brought into full play, so that the body-strengthening & disease-treating performance and growth promotion performance of the microecological feed additive for cows can be improved, and recessive mastitis of the cows can be effectively treated and prevented, thereby completely replacing the use of antibiotics.
Description
Technical field
The present invention relates to the animal and fowl fodder field, relate in particular to a kind of ox feed with Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives.
Background technology
Bovine subclinical mastitis is a kind of serious disease common, complicated, that can cause the dairy heavy losses, and what not only have a strong impact on cow utilizes the time limit, galactopoiesis quantity and dairy quality, and is harmful to food sanitation and human health.For a long time, the cowboying family is adopted mostly and is prevented bovine subclinical mastitis to the antibiotic mode of the long-term feeding of milk cow.Microbiotic has kill bacteria and the effect that suppresses pathogenic microorganism, and therefore this feeding patterns has played the effect of certain prevention bovine subclinical mastitis in actual production.But; These microbiotic can Along with people's eat milk and various milk food prod entering human body; This has just caused antibiotic abuse indirectly; Might cause multiple drug-induced disease, even can produce new " super resistant organism ", thereby human life and health in this feeding patterns serious threat.
For fear of or reduce milk cow and feed the medium-term and long-term microbiotic that uses of process, microecological feed additive for milk cows arises at the historic moment.So-called microecological feed additive for milk cows is the active probiotic preparation of processing through special process to the useful and harmless active probiotic of milk cow.Microecological feed additive for milk cows adds auxiliary material as a kind of functional ox feed; Realized under the prerequisite that has no side effect, has no drug resistance, do not have pathogenicity bo, preventing bovine subclinical mastitis, not only can regulate milk cow intestinal microecology balance, improve milk cow growth rate, improve efficiency of feed utilization and can suppress the harmful intestinal tract mikrobe breeding, improve the milk cow health level, strengthen milk cow immunizing power.At present; In existing milk cattle cultivating, the kind of common microecological feed additive for milk cows has nearly hundred kinds in lactic acid bacteria formulation (comprising lactobacterium acidophilus, bifidus, streptococcus faecium etc.), bacillus preparation (comprising Bacillus subtilus, bacillus cereus, lichens bacillus etc.), fungi preparation (comprising aspergillus oryzae etc.), yeast preparation (comprising cereuisiae fermentum etc.) etc.Existing ox with the microbial forage additive production process in; Only use protein substance and/or glucide substratum mostly as active probiotic; Though bacterial strain quantitatively can satisfy the demands; But the activity of most of bacterial strains is not high, the field planting performance in enteron aisle a little less than, so active probiotic as milk cow feed the effect that should bring into play receive very big restriction; Simultaneously, active probiotic only can strengthen the physique of milk cow to a certain extent, and the effect of its treatment and prevention bovine subclinical mastitis is not fairly obvious, and therefore, existing milk cow microbial forage additive can not replace antibiotic use fully.
Summary of the invention
The embodiment of the invention provides a kind of ox feed with Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives; So that significantly improve the active and field planting performance in enteron aisle of the bacterial strain of active probiotic; And make by its fodder additives that makes and be rich in traditional Chinese medicine ingredients; The activeconstituents that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to; Performance and promotes growth performance thereby the strong body that improves microecological feed additive for milk cows is cured the disease are effectively treated and are prevented bovine subclinical mastitis, and then replace antibiotic use fully.
The objective of the invention is to realize through following technical scheme:
A kind of ox feed is with Chinese medicine probiotic bacterium solid medium, and its composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid; Its each composition accounts for total mass ratio:
Wheat bran, 60～70 parts;
Dregs of beans, 15～20 parts;
Semen Maydis powder, 10～15 parts;
Brown sugar, 2～3 parts;
Traditional Chinese medicinal components, 2～3 parts;
Light calcium carbonate, 2～4 parts;
Yeast extract paste, 0.5～0.8 part;
Xanthohumic acid, 0.2～0.5 part;
Wherein, the composition of said traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight that each composition of traditional Chinese medicinal components accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Preferably, each composition of corresponding traditional Chinese medicinal components accounts for the parts by weight of traditional Chinese medicinal components total amount and is:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
Preferably, this ox feed uses each composition of Chinese medicine probiotic bacterium solid medium to account for total mass ratio to be:
Wheat bran, 67.8 parts;
Dregs of beans, 15 parts;
Semen Maydis powder, 10 parts;
Brown sugar, 2 parts;
Traditional Chinese medicinal components, 2 parts;
Light calcium carbonate, 2.5 parts;
Yeast extract paste, 0.5 part;
Xanthohumic acid, 0.2 part.
A kind of ox feed comprises with the cultural method of Chinese medicine active probiotic:
Steps A: prepare the ox feed with Chinese medicine probiotic bacterium solid medium with the moity of Chinese medicine probiotic bacterium solid medium according to the ox feed described in the technique scheme;
Step B: the ox feed is mixed into the substratum mixture with Chinese medicine probiotic bacterium solid medium and water and stirs, and the mass percent of moisture reaches 35% in the substratum mixture;
Step C: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to substratum mixture sterilization 20 minutes;
Step D: with active probiotic concentration is the active probiotic seed liquor of every milliliter 2,000,000,000 strain, is that 1: 1 mixed becomes active probiotic solid state fermentation seed with corn cob meal according to mass ratio;
Step e: active probiotic solid state fermentation seed is inoculated in the described substratum mixture by predetermined inoculative proportion;
Step F: the substratum mixture that will inoculate after accomplishing is put into culture vessel, and under 37 ℃, carries out sealing and fermenting, when the temperature in the culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, fermentation is accomplished when said culture vessel temperature no longer rises.
Preferably, predetermined accordingly inoculative proportion is: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1: 5 to 3: 10.
A kind of cattle feed addictive comprises: contain the Chinese medicine active probiotic according to the ox feed described in the technique scheme with what the cultural method of Chinese medicine active probiotic was cultivated.
Technical scheme by the invention described above provides can be found out; The ox feed that the embodiment of the invention provides adopts tens flavor Chinese medicines as the important component part of ox feed with Chinese medicine probiotic bacterium solid medium with Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives; Both improved the performance of the activeconstituents pharmacological action in the Chinese medicine; Make the activity of its active probiotic of turning out be higher than the active probiotic that other culture medium culturing goes out again, and strengthened the field planting ability of probiotics in enteron aisle.Because Chinese medicine and active probiotic be to immune synergy, the active probiotic of therefore breeding with Chinese medicine is as fodder additives for ox, and its immunological competence is considerably beyond other series products.We cultivate active probiotic through modern microecology principle is combined with the traditional Chinese medical science with traditional Chinese medicinal components, use the Chinese medicine probiotics thereby produce a kind of antibiotic no drug residue, prophyiaxis and promoting growth, ox with low cost of replacing fully.
Description of drawings
In order to be illustrated more clearly in the technical scheme of the embodiment of the invention; The accompanying drawing of required use is done to introduce simply in will describing embodiment below; Obviously, the accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skill in the art; Do not paying under the capable prerequisite of creative work, can also obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is ox feed that the embodiment of the invention the provided schematic flow sheet with the cultural method of Chinese medicine active probiotic.
Embodiment
Following combines the accompanying drawing in the embodiment of the invention, and the technical scheme in the embodiment of the invention is carried out clear, intactly description, and obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills belong to protection scope of the present invention not making the every other embodiment that is obtained under the creative work prerequisite.
Make a detailed description in the face of the ox feed that the embodiment of the invention provided with Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives down.
(1) the ox feed is with Chinese medicine probiotic bacterium solid medium
A kind of ox feed is with Chinese medicine probiotic bacterium solid medium, and its concrete composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid; Its each composition accounts for total mass ratio: wheat bran, 60～70 parts; Dregs of beans, 15～20 parts; Semen Maydis powder, 10～15 parts; Brown sugar, 2～3 parts; Traditional Chinese medicinal components, 2～3 parts; (precipitated chalk is claimed but also claimed to light calcium carbonate not only to light calcium carbonate, is the trickle lime carbonate of the particle of settling volume between 2.4～2.8mL/g that makes with the chemical process method; In the industrial production at home; Light calcium carbonate adopts carborization to make mostly---the calcining of raw materials such as Wingdale is generated lime and carbonic acid gas; Adding entry again makes lime become milk of lime; Feed carbonic acid gas then and make the milk of lime carbonization generate ZG 301, at last to ZG 301 dewater, drying and pulverization process get final product), 2～4 parts; Yeast extract paste, 0.5～0.8 part; Xanthohumic acid, 0.2～0.5 part.Adopt wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid substratum among the present invention as active probiotic; These materials are not only originated abundant, with low cost; And be more suitable for cultivation, packing and the preservation of active probiotic than liquid nutrient medium, therefore be suitable for industrialized production and utilization more.
Wherein, the composition of corresponding traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight that each composition of traditional Chinese medicinal components accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Particularly; In actual production is used; For many-sided factors such as comprehensive cost of manufacture and use propertieies, this ox feed accounts for total mass ratio with each composition of Chinese medicine probiotic bacterium solid medium can select the scheme in the following table 1 for use, and the embodiment one during wherein this is shown is scheme most preferably:
Table 1:
| Composition | Embodiment one | Embodiment two | Embodiment three | Embodiment four |
| Wheat bran | 67.8 | 61.8 | 63.8 | 64.8 |
| Dregs of beans | 15 | 18 | 17 | 16 |
| Semen Maydis powder | 10 | 12 | 10 | 12 |
| Brown sugar | 2 | 3 | 2.5 | 2 |
| Traditional Chinese medicinal components | 2.5 | 2.5 | 2.5 | 2.5 |
| Light calcium carbonate | 2 | 2 | 3 | 2 |
| Yeast extract paste | 0.5 | 0.5 | 0.7 | 0.5 |
| Xanthohumic acid | 0.2 | 0.2 | 0.5 | 0.2 |
In above table, the composition of corresponding traditional Chinese medicinal components, and the parts by weight that each composition accounts for the traditional Chinese medicinal components total amount can select the scheme in the following table 2 for use, wherein the unit in this table is weight part, and embodiment a is scheme most preferably:
Table 2:
| The traditional Chinese medicine ingredients title | Instance a | Instance b | Instance c | Instance d |
| The Radix Astragali | 80 | 70 | 75 | 60 |
| Radix Codonopsis | 80 | 60 | 65 | 75 |
| Taraxacum | 80 | 75 | 70 | 60 |
| The root of large-flowered skullcap | 50 | 40 | 55 | 60 |
| Rhizome of chuanxiong | 20 | 15 | 15 | 10 |
| Semen Vaccariae | 50 | 40 | 55 | 60 |
| Play both sides of the street | 50 | 60 | 55 | 40 |
| Radix bupleuri | 50 | 60 | 70 | 80 |
| Radix Angelicae Sinensis | 60 | 55 | 50 | 40 |
| Motherwort Herb | 60 | 50 | 55 | 70 |
| Kelp | 120 | 110 | 100 | 90 |
| Radix Glycyrrhizae | 50 | 30 | 40 | 45 |
Further, the present invention is described in the efficacy of traditional Chinese medicines for the various components: Astragalus - sweet and warm, qi Sun, the active ingredient of Astragalus polysaccharides can improve immunity; Codonopsis - sweet nature, Buzhongyiqi , and stomach, the active ingredient saponins can improve immunity; dandelion - sweet natured can detoxify, and reducing swelling with dandelion sterols, choline, inulin and other ingredients and skullcap common mastitis suppression of harmful bacteria (Kim aureus and Streptococcus pyogenes); skullcap - heat and dryness, cooling blood detoxification; Sichuan dome - blood circulation of qi, yin, circulation, Sichuan dome of blood gas medicine, let the blood fill the does not stagnate; Vaccaria - promoting blood circulation longitude, under the breast carbuncle, which contains triterpenoid saponins, glycosides; Passepartout - blood circulation, Qufeng, diuresis through longitude; Bupleurum - taste bitter, slightly cold, hepatobiliary classics, there Shugan and stomach, Sun's functional, antibacterial and antiviral and immunomodulatory functions; Angelica - sweet and warm, not only blood, but also blood, ferulic acid, the active ingredient Sodium can activate macrophages, immune function play; Motherwort - stasis, containing a variety of alkaloids and organic selenium, can improve the body's immune system; Khumbu - Pinggan, suppressing gas relieves congestion, organic iodine content High and rich in Tongru factors can increase milk yield; licorice - with detoxification, to reconcile the various drugs, their active ingredient glycyrrhizin in cell immunomodulator.The mentioned traditional Chinese medicinal components of the present invention is the growth characteristic according to milk cow, the treatment of bovine subclinical mastitis and the breed demand in prevention mechanism and the milk cattle cultivating process; The drug matching principle of adopt monarch, minister, helping, make; It is formulated to choose a few flavor Chinese medicines that can promote milk cow digestion and growth, can strengthen the milk cow immunity of organisms, can improve the milk cow resistance against diseases respectively---and at first select for use the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis as monarch drug in a prescription; Improve animal body immunizing power, reach the purpose of benefiting qi and nourishing blood: select for use taraxacum, the root of large-flowered skullcap, radix bupleuri to improve its anti-bacteria and anti-virus ability simultaneously; And select rhizome of chuanxiong, Semen Vaccariae for use, play both sides of the street, Motherwort Herb, kelp breast promoting blood circulation and removing blood stasis, logical and improve milk yield; Select for use the Radix Glycyrrhizae not only can be clearing heat and detoxicating and can also be in harmonious proportion all the other all medicines at last.This shows that traditional Chinese medicinal components provided by the present invention not only can promote the speed of growth of milk cow, and can strengthen the disease resistance ability of milk cow, thereby can effectively treat and prevent bovine subclinical mastitis.
(2) the ox feed is with the cultural method of Chinese medicine active probiotic
As shown in Figure 1, a kind of ox feed specifically comprises following step with the cultural method of Chinese medicine active probiotic:
Steps A: prepare the ox feed with Chinese medicine probiotic bacterium solid medium with the moity of Chinese medicine probiotic bacterium solid medium according to the ox feed described in the technique scheme;
Step B: the ox feed is mixed into the substratum mixture with Chinese medicine probiotic bacterium solid medium and water and stirs, and the mass percent of moisture reaches 35% in the substratum mixture;
Step C: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to substratum mixture sterilization 20 minutes;
In practical application, preferably select for use under 121 ℃ the substratum mixture was sterilized 20 minutes, can be killed fully with the harmful bacterium in the Chinese medicine probiotic bacterium solid medium to guarantee the ox feed.
Step D: with active probiotic concentration is the active probiotic seed liquor of every milliliter 2,000,000,000 strain, is that 1: 1 mixed becomes active probiotic solid state fermentation seed with corn cob meal according to mass ratio;
Wherein, corresponding corn cob meal preferably passes through Micvowave Sterilization (the microwaves corresponding sterilization is meant that adopting frequency is the heat energy killing microorganisms that produces of the microwave irradiation of 300MHz～300000MHz and the method for gemma.This method is fit to the sterilization of liquid and solid materials, and solid materials is had desiccation) after corn cob meal, not have unwanted bacteria in the assurance institute synthetic active probiotic solid state fermentation seed.
Step e: active probiotic solid state fermentation seed is inoculated in the described substratum mixture by predetermined inoculative proportion;
Wherein, predetermined accordingly inoculative proportion is: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1: 5 to 3: 10; Thereby neither waste the active probiotic seed liquor, can let the ox feed cultivate all good active probiotics of quantity quality again with Chinese medicine probiotic bacterium solid medium.
Step F: the substratum mixture that will inoculate after accomplishing is put into culture vessel, and under 37 ℃, carries out sealing and fermenting, when the temperature in the culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, fermentation is accomplished when said culture vessel temperature no longer rises; The active probiotic bacterial strain number of this moment reaches more than every milliliter 4,500,000,000 strain.In general, sealing and fermenting is 5～7 days usually, and the culture vessel temperature is no longer risen.
Particularly, ox feed provided by the present invention with the cultural method of Chinese medicine active probiotic go for to subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum 、 Chan ?in candiyeast, black mold, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and the bifidus bacillus one or more cultivate.
(3) cattle feed addictive that makes with the cultural method of Chinese medicine active probiotic by the ox feed
A kind of cattle feed addictive comprises: contain the Chinese medicine active probiotic according to the described ox feed of technique scheme with what the cultural method of Chinese medicine active probiotic was cultivated.
Wherein, can comprise in the corresponding cattle feed addictive subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum 、 Chan ?candiyeast, black mold, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and bifidus bacillus isoreactivity probiotic bacterium.
Need to prove that strain culturing equipment used in the present invention and the strain culturing technology of not describing in detail all can adopt strain culturing equipment general in the prior art and strain culturing technology, do not remake detailed description in this application.Various Chinese medicines described in the present invention all can be buied in each big Chinese medicine pharmacy easily, therefore this are no longer detailed.The former bacterial strain of various active probiotic bacterium described in the present invention all can be introduced from Chinese common micro-organisms culture presevation administrative center (CGMCC) or agriculture microbial strains preservation administrative center (ACCC), with existing state and the survival quality of guaranteeing each bacterial classification.
It is thus clear that; The enforcement of the embodiment of the invention can significantly improve the active and field planting performance in enteron aisle of bacterial strain of active probiotic; And make by its fodder additives that makes and be rich in traditional Chinese medicine ingredients, the activeconstituents that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to, performance and promotes growth performance thereby the strong body of raising microecological feed additive for milk cows is cured the disease; Effectively treat and the prevention bovine subclinical mastitis, and then replace antibiotic use fully.
The above; Be merely the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technician who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (6)
1. an ox feed is characterized in that its composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid with Chinese medicine probiotic bacterium solid medium; Its each composition accounts for total mass ratio:
Wheat bran, 60～70 parts;
Dregs of beans, 15～20 parts;
Semen Maydis powder, 10～15 parts;
Brown sugar, 2～3 parts;
Traditional Chinese medicinal components, 2～3 parts;
Light calcium carbonate, 2～4 parts;
Yeast extract paste, 0.5～0.8 part;
Xanthohumic acid, 0.2～0.5 part;
Wherein, the composition of said traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight that each composition of traditional Chinese medicinal components accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
2. ox feed according to claim 1 is characterized in that with Chinese medicine probiotic bacterium solid medium the parts by weight that each composition of said traditional Chinese medicinal components accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
3. ox feed according to claim 1 and 2 is characterized in that with Chinese medicine probiotic bacterium solid medium each composition of this ox feed use Chinese medicine probiotic bacterium solid medium accounts for total mass ratio and is:
Wheat bran, 67.8 parts;
Dregs of beans, 15 parts;
Semen Maydis powder, 10 parts;
Brown sugar, 2 parts;
Traditional Chinese medicinal components, 2 parts;
Light calcium carbonate, 2.5 parts;
Yeast extract paste, 0.5 part;
Xanthohumic acid, 0.2 part.
4. an ox feed is characterized in that with the cultural method of Chinese medicine active probiotic, comprising:
Steps A: prepare the ox feed with Chinese medicine probiotic bacterium solid medium with the moity of Chinese medicine probiotic bacterium solid medium according to the ox feed described in the claim 1 to 3;
Step B: the ox feed is mixed into the substratum mixture with Chinese medicine probiotic bacterium solid medium and water and stirs, and the mass percent of moisture reaches 35% in the substratum mixture;
Step C: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to substratum mixture sterilization 20 minutes;
Step D: with active probiotic concentration is the active probiotic seed liquor of every milliliter 2,000,000,000 strain, is that 1: 1 mixed becomes active probiotic solid state fermentation seed with corn cob meal according to mass ratio;
Step e: active probiotic solid state fermentation seed is inoculated in the described substratum mixture by predetermined inoculative proportion;
Step F: the substratum mixture that will inoculate after accomplishing is put into culture vessel, and under 37 ℃, carries out sealing and fermenting, when the temperature in the culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, fermentation is accomplished when said culture vessel temperature no longer rises.
5. ox feed according to claim 4 is characterized in that with the cultural method of Chinese medicine active probiotic described predetermined inoculative proportion is: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1: 5 to 3: 10.
6. a cattle feed addictive is characterized in that, comprising: contain the Chinese medicine active probiotic according to the described ox feed of claim 4 to 5 with what the cultural method of Chinese medicine active probiotic was cultivated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102768854A CN102367416B (en) | 2011-09-16 | 2011-09-16 | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102768854A CN102367416B (en) | 2011-09-16 | 2011-09-16 | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102367416A true CN102367416A (en) | 2012-03-07 |
| CN102367416B CN102367416B (en) | 2013-12-25 |
Family
ID=45760009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102768854A Expired - Fee Related CN102367416B (en) | 2011-09-16 | 2011-09-16 | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102367416B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103652498A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Biological cow feed and preparation method thereof |
| CN104095149A (en) * | 2014-08-14 | 2014-10-15 | 郭庆 | Preparation method of traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows |
| CN104435391A (en) * | 2014-12-29 | 2015-03-25 | 河南天纳图生物科技有限公司 | Fermentation traditional Chinese medicine for activating blood and increasing milk for livestock and preparation method thereof |
| CN104547507A (en) * | 2013-10-14 | 2015-04-29 | 新疆农垦科学院 | Traditional Chinese medicine prescription for reducing number of somatic cells in dairy cow milk and promoting milk production |
| CN104642813A (en) * | 2015-03-12 | 2015-05-27 | 武汉如意食用菌生物高科技有限公司 | Ruminant feed and preparation method of ruminant feed |
| CN106260542A (en) * | 2016-07-29 | 2017-01-04 | 河南德邻生物制品有限公司 | A kind of lactating sow Chinese medicine microbial fermentation preparation and preparation method thereof |
| CN106509409A (en) * | 2015-09-14 | 2017-03-22 | 天津昌农科技有限责任公司 | Dairy cow fermentation feed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406244A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Feedstuff additive for immunity enhancement of livestock and poultry |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
| CN101700332A (en) * | 2009-11-04 | 2010-05-05 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composite and premixed feed capable of increasing production performance of livestock and poultry |
-
2011
- 2011-09-16 CN CN2011102768854A patent/CN102367416B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406244A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Feedstuff additive for immunity enhancement of livestock and poultry |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
| CN101700332A (en) * | 2009-11-04 | 2010-05-05 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composite and premixed feed capable of increasing production performance of livestock and poultry |
Non-Patent Citations (1)
| Title |
|---|
| 兰时乐等: "中草药饲料添加剂发酵菌种的筛选与发酵条件研究", 《中国饲料》, no. 15, 31 December 2010 (2010-12-31), pages 13 - 17 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547507A (en) * | 2013-10-14 | 2015-04-29 | 新疆农垦科学院 | Traditional Chinese medicine prescription for reducing number of somatic cells in dairy cow milk and promoting milk production |
| CN104547507B (en) * | 2013-10-14 | 2017-11-28 | 新疆农垦科学院 | It is a kind of to reduce somatic number in milk cow's milk and promote newborn Chinese prescription |
| CN103652498A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Biological cow feed and preparation method thereof |
| CN103652498B (en) * | 2013-12-18 | 2015-08-05 | 青岛百瑞吉生物工程有限公司 | A kind of biological forage for dairy cattle and preparation method thereof |
| CN104095149A (en) * | 2014-08-14 | 2014-10-15 | 郭庆 | Preparation method of traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows |
| CN104095149B (en) * | 2014-08-14 | 2016-06-15 | 郭庆 | A kind of preparation method preventing and treating bovine subclinical mastitis Traditional Chinese drug microecology additive |
| CN104435391A (en) * | 2014-12-29 | 2015-03-25 | 河南天纳图生物科技有限公司 | Fermentation traditional Chinese medicine for activating blood and increasing milk for livestock and preparation method thereof |
| CN104642813A (en) * | 2015-03-12 | 2015-05-27 | 武汉如意食用菌生物高科技有限公司 | Ruminant feed and preparation method of ruminant feed |
| CN106509409A (en) * | 2015-09-14 | 2017-03-22 | 天津昌农科技有限责任公司 | Dairy cow fermentation feed |
| CN106260542A (en) * | 2016-07-29 | 2017-01-04 | 河南德邻生物制品有限公司 | A kind of lactating sow Chinese medicine microbial fermentation preparation and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102367416B (en) | 2013-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN102366024B (en) | Production method of pig feed additive containing traditional Chinese medicine and active probiotics | |
| CN108813098B (en) | Microbial fermentation composite antibiotic-free feed containing natural antibiotic components | |
| CN102366021B (en) | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics | |
| CN102657287B (en) | Compound feed addictive with bacteria and oxidation resisting functions | |
| CN106798862B (en) | Traditional Chinese medicine microecological preparation for improving immunity of chicks, preparation method and use method thereof | |
| CN102366025B (en) | Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics | |
| CN103636946B (en) | Preparation method and application of Chinese herbal medicine immunological enhancer for finless eel | |
| CN102367418B (en) | Traditional Chinese medicine probiotics liquid medium used for pig feed, culturing method and feed additive | |
| CN102367416B (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN102696884A (en) | Composite feed additive product | |
| CN102669435A (en) | Feed additive product | |
| CN102367415A (en) | Traditional Chinese medicine probiotics liquid culture medium for pig feed, culture method and feed additive | |
| CN104304748A (en) | Probiotic fermented feed additive for dairy cattle | |
| CN110495522A (en) | A kind of feed Chinese medicine microecological preparation | |
| CN108991261A (en) | A kind of preparation method for the pannage that ferments | |
| CN104082528B (en) | A kind of feed addictive and preparation method thereof | |
| CN113616715B (en) | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof | |
| CN102367420B (en) | Traditional Chinese medicine probiotic liquid culture medium used in cow forage, culturing method, and forage additive | |
| CN102763772A (en) | Functional feed additive | |
| CN102367419B (en) | Traditional Chinese medicine probiotics liquid culture medium for poultry feed, culture method and feed additive | |
| CN102367417B (en) | Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive | |
| CN109221697A (en) | Weaned piglets probiotic feed and preparation method thereof | |
| CN106215011A (en) | A kind of preparation method of the Chinese herbal medicinal active fermentation broth preventing swine fever | |
| CN103385368B (en) | Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190812 Address after: 116000 No. 229-4 Zhongshan District, Dalian City, Liaoning Province Patentee after: Hualin (Liaoning) Biotechnology Co.,Ltd. Address before: 116201 No. 182 Nansha Street, Shahekou District, Dalian City, Liaoning Province, 6-1 Patentee before: Wang Songlin |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131225 |