CN104095149A - Preparation method of traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows - Google Patents
Preparation method of traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows Download PDFInfo
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Abstract
Disclosed is a preparation method of a traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows. The preparation method comprises performing superfine grinding on eucommia ulmoides leaves, ginkgo leaves, purple perilla, Chinese pulsatilla roots, coneflowers, humulus scandens, shrimp shells, milkvetch roots and licorice roots; adding water and boiling through the high heat; performing soft file insulation; performing cooling and filtration; collecting filtrate; adding white sugar and boiling; cooling down to the room temperature and performing lactobacillus, tea fungi and yeast inoculation to obtain the micro-ecological additive through fermentation; enabling the cows to drink the obtained micro-ecological additive in proportion. According to the traditional Chinese medicine micro-ecological additive, the functions of heat clearing and detoxification, sterilization and anti-inflammation, lactation promotion and the like are achieved, Chinese herbal medicine active materials, active small peptides, lactic acid and probiotics are rich, the amino acid is rich and balanced, the traditional antibiotic treatment method is replaced, and the harm of antibiotics to the health of the cows and antibiotic residues in the milk are reduced. According to the preparation method of the traditional Chinese medicine micro-ecological additive for the prevention and treatment of the subclinical mastitis of the cows, the use is simple and convenient, drug residues are not produced, any toxic and side effect is not produced, the viable bacteria content is high, the preparation method is suitable for industrialized production, and the market prospects are good.
Description
Technical field
The invention belongs to feed microbe fermentation technical field, relate to specifically a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive.
Background technology
Mastitis for milk cows (Mastitis) is that mammary gland is subject to of science variation of a kind of inflammatory disease that the stimulations such as physics, chemistry, microorganism occur, its main feature is that milk generation physicochemical property and bacteriology change, being one of milk cow common disease, is also to one of the most great disease of milk cow disserve to produce.Mastitis for milk cows, according to having or not clinical symptoms, is divided into clinic mastitis (Clinical Mastitis) and recessive mastitis (Subclinical Mastitis) two classes.Cause pathogeny imcrobe infection is its main pathogenic clinically, and pathogenic microorganism mainly comprises staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, Escherichia coli, streptococcus uberis, Friedlander's bacillus etc.The microorganism infecting is because of different from different places, staphylococcus wherein, and streptococcus, Enterobacteriaceae is more common.And recessive mastitis is often ignored treatment then is developed into clinic mastitis because do not show clinical symptoms.
Milk cow suffers from after recessive mastitis, and the toxin that contains a large amount of inflammatory factors, pathogenic microorganism and generation thereof in the milk producing, can endanger the healthy of consumer, particularly for the elderly and infant, endangers maximum.Due to heavy dose of, use various antibiotic medicines for a long time, cause Ruzhong to have residual antibiotic.When people are edible, containing after antibiotic breast, can cause allergic reaction as fash, discomfort, severe patient generation anaphylactic shock.More people are antibiotic Bovine Mastitis Milk because long-term drinking remains, and makes bacterium in body produce drug resistance, brings larger difficulty to the treatment of disease.
At present, antibiotic is the conventional medicine for the treatment of mastitis, but due to long-term a large amount of use of antibiotic, causes on the one hand antibody-resistant bacterium to increase, the food-safety problem that Residue of Antibiotics in Milk brings on the other hand also more and more receives people's concern, " melamine " " malicious milk powder " event.Therefore, safe prevention mastitis for milk cows more and more causes people's attention.
With Chinese herbal treatment mastadenitis of cow, become popular research topic in recent years, Chinese herbal medicine powder for Yue Chunwang etc. " newborn scorching health " treatment clinic mastitis, cure rate is 93.3% (28/30); The Chinese medicine nipple that 2 prescriptions of use compound coptis chinensis and compound indigowoad leaf such as the straight U.S. of Kimura are made is respectively dipped agent treatment recessive mastitis and is compared with control group, and recessive mastitis negative rate is 53.6% and 63.9%; The application herbal injections " Ru Yuankang " such as Zhou Xuzheng adopt breast administration to accept the sick ox of all kinds of clinic mastitis for medical treatment 1638 times, 2203 Ge Ru districts, and average cure rate is 93.3%, total effective rate is 96.7%.
Above Chinese medicine compound prescription is mainly powder, decoction, injection, and wherein powder and decoction dosage are large, because its taste is special, some animals are difficult to gavage, and that injection stimulates animal is very large, to animal produce certain stress, increased its difficulty at clinical expansion.
And the Chinese medicine microecological preparation of preparation that Chinese medicine is fermented together with microorganism has no report in prior art.
Summary of the invention
The object of the invention is in order to address the above problem, a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive is provided, there is the effects such as clearing heat and detoxicating, bactericidal antiphlogistic, the logical breast of lactagogue, be rich in Chinese herbal medicine active material, active small peptide, lactic acid and probio, there is amino acid and enrich balance, replace traditional antibiotic therapy method, reduce antibiotic to the harm of milk cow health and antibiotic the feature such as residual in Ruzhong.
For achieving the above object, the method that the present invention adopts is as follows:
A kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, it is characterized in that: it is to form by the Chinese medicine of following weight portion: folium cortex eucommiae 120～150, ginkgo leaf 120～150, purple perilla 100～130, the Chinese bulbul 100～130, Echinacea 100～120, humulus grass 100～120, shrimp shell 80～100, the Radix Astragali 60～80, Radix Glycyrrhizae 40～60, ultramicro grinding, adding water intense fire boils, slow fire insulation, cold filtration, collect filtrate, add white sugar to boil, coolingly be down to inoculating lactobacillus after normal temperature, fermented tea and saccharomycetes to make fermentation gained probiotics, gained probiotics is drunk to milk cow in proportion.
Described a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, it is characterized in that each Chinese herbal medicine ultramicro grinding, take the Chinese herbal medicine of weight portion, the water that adds 6～8 times of amounts of Chinese herbal medicine gross weight, soak 30～60min, intense fire boils, slow fire insulation 30～60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 4～5 times of amounts again, and intense fire boils, slow fire insulation 30～60min, and cooling Floor 12 filtered through gauze, merges twice filtrate.
Described a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, it is characterized in that take that 100 Kg filtrates are as the interpolation white sugar 15～20Kg of unit, and boil 5～10min, be cooled to normal temperature, inoculating lactobacillus, fermented tea and saccharomycete preparation 15～30Kg, ferment to till pH6.0-7.0.
Described a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, is characterized in that fermentation is to carry out in aseptic fermenting cellar.
Described a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, is characterized in that fermentation time is about 3～7 days, and fermentation temperature is 35～37 ℃.
Described a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, is characterized in that gained zymotic fluid dilutes 5～10 times and directly to milk cow, drinks afterwards.
Choose parity, age, the lactation moon close, health is good, breast detects the negative control group of 10 of negative healthy cows (40Ge Ru district) without clinical abnormal, milk without the CMT of naked eyes visible change, CMT detects 20 of the milk cows that positive somatic number more than 500,000 (suffers from recessive mastitis) at every milliliter of breast, be divided at random 2 groups, 5 (20Ge Ru districts of positive controls, 14 of positive udder region) and 15 of Chinese medicine micro-ecological additive treatment groups (60, newborn district, 49 of positive udder region).Treatment group milk cow is early, squeezed after milk evening, by 5 times of Chinese medicine micro-ecological additive dilutions, allows milk cow drink, every day 2 times, and continuous use 5 days, positive controls is not treated, and negative control group is diluted 10 times by Chinese medicine micro-ecological additive, allows milk cow drink.Beneficial effect of the present invention is following 2 points: 1), from table 1 result, show the cure rate of Chinese medicine micro-ecological additive clinical practice and be efficiently respectively 73.5% and 91.8%, reach good result for the treatment of, not morbidity of negative control group simultaneously, has reached preventive effect; 2), from table 2 result, show that Chinese medicine micro-ecological additive treatment group can improve the lactation amount of ill milk cow fast through the treatment of 5 days, improved 19.69%, suitable with negative control group, reach the lactation amount standard of normal health milk cow.
Preparation method of the present invention is simple, easy to use, drug residue free, without any side effects, viable bacteria content is high, the stimulation that milk cow is caused, stress be little, economical and practical, for production practices provide good traditional Chinese medicine formula and the micro-ecological additive of preventing and treating mastitis for milk cows, the final significant problem that solves " antibacterial milk " in production reality, be applicable to suitability for industrialized production, have good market prospects.
Table 1 Chinese medicine micro-ecological additive is treated cow subclinical mastitis curative effect and cure rate.
Table 2 Chinese medicine micro-ecological additive is on the impact of cow subclinical mastitis lactation amount [Kg. (d. head)
-1].
| Group | 0 d | 5 d |
| Negative control group | 20.57±0.30 | 21.63±0.33 |
| Positive controls | 16.86±0.45 | 17.02±0.25 |
| Chinese medicine micro-ecological additive treatment group | 17.16±0.41 | 20.85±0.22 |
The specific embodiment
embodiment 1
A preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, comprises the following steps:
Take (Kg) Chinese herbal medicine of following weight portion: folium cortex eucommiae 120, ginkgo leaf 120, purple perilla 100, the Chinese bulbul 100, Echinacea 100, humulus grass 100, shrimp shell 80, the Radix Astragali 60, Radix Glycyrrhizae 40, ultramicro grinding.
The preparation of zymotic fluid: add the water of 6 times of amounts of Chinese herbal medicine gross weight, soak 30min, intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 4 times of amounts again, and intense fire boils, slow fire insulation 300min, and cooling Floor 12 filtered through gauze, merges twice filtrate.100 Kg filtrates are that unit adds white sugar 15Kg, and boil 5min, are cooled to normal temperature, inoculating lactobacillus, fermented tea and saccharomycete preparation 15Kg, and fermentation is to carry out in aseptic fermenting cellar, and fermentation temperature is 35 ℃, and fermentation 7d ferments to till pH6.0.
Gained zymotic fluid dilutes 5 times and directly to milk cow, drinks afterwards.
embodiment 2
A preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, comprises the following steps:
Take following weight (Kg) Chinese herbal medicine: folium cortex eucommiae 150, ginkgo leaf 150, purple perilla 130, the Chinese bulbul 130, Echinacea 120, humulus grass 120, shrimp shell 100, the Radix Astragali 80, Radix Glycyrrhizae 60, ultramicro grinding.
The preparation of zymotic fluid: add the water of 8 times of amounts of Chinese herbal medicine gross weight, soak 60min, intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 5 times of amounts again, and intense fire boils, slow fire insulation 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate.100 Kg filtrates are that unit adds white sugar 20Kg, and boil 10min, are cooled to normal temperature, inoculating lactobacillus, fermented tea and saccharomycete preparation 30Kg, and fermentation is to carry out in aseptic fermenting cellar, 37 ℃ of fermentation temperatures, fermentation time 7d, ferments to till pH7.0.
Gained zymotic fluid dilutes 10 times and directly to milk cow, drinks afterwards.
Claims (6)
1. a preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive, it is characterized in that: it is to form by the Chinese medicine of following weight portion: folium cortex eucommiae 120～150, ginkgo leaf 120～150, purple perilla 100～130, the Chinese bulbul 100～130, Echinacea 100～120, humulus grass 100～120, shrimp shell 80～100, the Radix Astragali 60～80, Radix Glycyrrhizae 40～60, ultramicro grinding, adding water intense fire boils, slow fire insulation, cold filtration, collect filtrate, add white sugar to boil, coolingly be down to inoculating lactobacillus after normal temperature, fermented tea and saccharomycetes to make fermentation gained probiotics, gained micro-ecological additive is drunk to milk cow in proportion.
2. a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive according to claim 1, it is characterized in that each Chinese herbal medicine ultramicro grinding, take the Chinese herbal medicine of weight portion, the water that adds 6～8 times of amounts of Chinese herbal medicine gross weight, soak 30～60min, intense fire boils, slow fire insulation 30～60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 4～5 times of amounts again, and intense fire boils, slow fire insulation 30～60min, and cooling Floor 12 filtered through gauze, merges twice filtrate.
3. a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive according to claim 1, it is characterized in that take that 100 Kg filtrates are as the interpolation white sugar 15～20Kg of unit, and boil 5～10min, be cooled to normal temperature, inoculating lactobacillus, fermented tea and saccharomycete preparation 15～30Kg, ferment to till pH6.0-7.0.
4. a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive according to claim 1, is characterized in that fermentation is to carry out in aseptic fermenting cellar.
5. a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive according to claim 1, is characterized in that fermentation time is about 3～7 days, and fermentation temperature is 35～37 ℃.
6. a kind of preparation method who prevents and treats cow subclinical mastitis Chinese medicine micro-ecological additive according to claim 1, is characterized in that gained zymotic fluid dilutes 5～10 times and directly to milk cow, drinks afterwards.
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| CN104857174A (en) * | 2015-05-26 | 2015-08-26 | 郭庆 | Preparation method of traditional Chinese medicine micro-ecological oral preparation for preventing and treating staphylococcosis in chickens |
| CN104938822A (en) * | 2015-06-06 | 2015-09-30 | 安徽极速时代动物营养有限公司 | Feed additive for reducing cow body cells and treating recessive mastitis and preparation method of feed additive |
| CN104997867A (en) * | 2015-08-12 | 2015-10-28 | 郭庆 | Method for preparing traditional Chinese medicine micro-ecology oral preparation for preventing ascariasis |
| CN105410368A (en) * | 2015-11-22 | 2016-03-23 | 衡阳市富矿饲料添加剂有限公司 | Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof |
| RU2624868C2 (en) * | 2015-09-14 | 2017-07-07 | федеральное государственное автономное образовательное учреждение высшего образования "Северо-Кавказский федеральный университет" | Preparation method of the complex gel for mastitis treatment and prevention in cows and complex gel on its basis |
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| RU2624868C2 (en) * | 2015-09-14 | 2017-07-07 | федеральное государственное автономное образовательное учреждение высшего образования "Северо-Кавказский федеральный университет" | Preparation method of the complex gel for mastitis treatment and prevention in cows and complex gel on its basis |
| CN105410368A (en) * | 2015-11-22 | 2016-03-23 | 衡阳市富矿饲料添加剂有限公司 | Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof |
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