CN102366021B - Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics - Google Patents

Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics Download PDF

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CN102366021B
CN102366021B CN2011102764143A CN201110276414A CN102366021B CN 102366021 B CN102366021 B CN 102366021B CN 2011102764143 A CN2011102764143 A CN 2011102764143A CN 201110276414 A CN201110276414 A CN 201110276414A CN 102366021 B CN102366021 B CN 102366021B
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parts
culture medium
seed
bacterial strain
chinese medicinal
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CN2011102764143A
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CN102366021A (en
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汪松林
李羽
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汪松林
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Abstract

The invention discloses a manufacturing method of a poultry feed additive containing traditional Chinese medicinal activity probiotics, comprising the following steps of: carrying out single stain quantity enlarge culture on the original strain of every poultry activity probiotic; mixing equal weight first-level seed liquids of each poultry activity probiotic, inoculating to a medium in a seed jar for mixing and culturing of a plurality of strains, and inoculating into a traditional Chinese medicinal liquid medium for the fermentation of the traditional Chinese medicine so as to prepare the poultry feed additive containing traditional Chinese medicinal activity probiotics. The embodiment provided by the invention can be implemented to greatly raise the strain activity of activity probiotics in the poultry feed additive and the colonization performance in intestinal tract, make the prepared poultry feed additive rich in traditional Chinese medicinal components and make the active components with pharmacological effects in the traditional Chinese medicinal components to fully perform, so as to raise the poultry feed additive's body strengthening and disease treating performance and growth promotion performance. Furthermore, antibiotics are completely replaced.

Description

A kind of manufacture method that contains the livestock fodder additives of active Chinese drug component probio
Technical field
The present invention relates to the animal and fowl fodder field, relate in particular to a kind of manufacture method that contains the livestock fodder additives of active Chinese drug component probio.
Background technology
In recent years, the communicable disease of family poultry was concentrated outburst, and poultry farming is suffered heavy losses.In order to strengthen the outburst of a poultry physique, a raising man poultry resistance against diseases, the extensive communicable disease of minimizing, many poultry producers have adopted one after another to the antibiotic feeding patterns of the long-term feeding of family poultry.Antibiotic has kill bacteria and suppresses the effect of pathogenic microorganisms, and therefore this feeding patterns has played certain protection effect in actual production.But, these antibiotic can enter human body along with the edible various poultry meat goods of people, and this has just caused antibiotic abuse indirectly, might cause multiple drug induced disease, even can produce new " super drug-fast bacteria ", thereby human life and health in this feeding patterns serious threat.
For fear of or reduce the poultry of being in and feed the medium-term and long-term antibiotic that uses of process, poultry is arisen at the historic moment with microbial forage additive.So-called poultry is the active probiotic preparation of making through special process to the useful and harmless active probiotic of poultry with microbial forage additive.Poultry is added auxiliary material with microbial forage additive as a kind of functional poultry feed, have have no side effect, have no drug resistance, without pathogenic characteristics, can regulate a poultry intestinal microecology balance, improve family's poultry growth rate, improve efficiency of feed utilization, suppress the harmful intestinal tract microorganism breeding, improve family's poultry general level of the health, strengthen the every household immunity of fowl.At present, in existing poultry farming, common poultry has nearly hundred kinds in lactobacillus preparation (comprising lactobacterium acidophilus, Bacillus bifidus, streptococcus fecalis etc.), bacillus preparation (comprising hay bacillus, bacillus cereus, B.lieheniformis etc.), fungi preparation (comprising aspergillus oryzae etc.), saccharomycete preparation (comprising brewer's yeast etc.) etc. with the kind of microbial forage additive.Use in the microbial forage additive production process existing poultry, mostly only use protein substance and/or glucide as the culture medium of active probiotic, although bacterial strain quantitatively can satisfy the demands, but the activity of most of bacterial strains is not high, field planting performance in enteron aisle a little less than, so the effect that active probiotic should be brought into play as animal and fowl fodder is very restricted; Simultaneously, active probiotic only can strengthen the physique of a poultry to a certain extent, can't strengthen the disease resistance ability of a poultry, does not more possess the disease treatment effect, and therefore, existing poultry microbial forage additive can not replace antibiotic use fully.
Summary of the invention
The embodiment of the invention provides a kind of manufacture method that contains the livestock fodder additives of active Chinese drug component probio, so that significantly improve the active and field planting performance in enteron aisle of the bacterial strain of active probiotic in the livestock fodder additives, and make the livestock fodder additives that makes be rich in traditional Chinese medicine ingredients, the active component that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to, performance and growth promotion performance thereby the strong body that improves livestock fodder additives is cured the disease, and then replace antibiotic use fully.
The objective of the invention is to be achieved through the following technical solutions:
A kind of manufacture method that contains the livestock fodder additives of active Chinese drug component probio comprises:
Steps A: choose the multiple poultry that equates bacterial strain quantity with the former bacterial strain of active probiotic, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, thereby obtain the primary seed solution that every kind of poultry is used active probiotic;
Wherein, described multiple poultry comprises in bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, candida utili, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds with active probiotic;
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out herb fermenting in the Chinese medicinal liquor culture medium, thereby make the livestock fodder additives that contains the active Chinese drug component probio;
Wherein, described Chinese medicinal liquor culture medium comprises 5%~8% brown sugar, 2%~3% traditional Chinese medicinal components, 0.2%~0.3% potassium dihydrogen phosphate, 0.3%~0.5% yeast extract and the water of surplus; Described traditional Chinese medicinal components is comprised of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60~80 parts, Radix Codonopsis: 60~80 parts, gynostemma pentaphylla: 60~80 parts, wilsonii: 60~80 parts,
The root of large-flowered skullcap: 50~80 parts, Radix Isatidis: 40~60 parts, the Chinese bulbul: 40~60 parts, honeysuckle: 40~60 parts,
Radix Angelicae Sinensis: 40~60 parts, hawthorn: 50~80 parts, dried orange peel: 50~80 parts, purslane: 40~60 parts,
Radix Glycyrrhizae: 30~50 parts.
As seen from the above technical solution provided by the invention, the manufacture method of the livestock fodder additives that contains the active Chinese drug component probio that the embodiment of the invention provides adopts kinds of traditional Chinese medicines is cultivated good facilitation to poultry growth and resistance against diseases active probiotic, thereby make the active component in the Chinese medicine give full play to pharmacological action, and the active probiotic that the activity that makes the active probiotic of turning out is turned out far above ordinary culture medium has also strengthened the colonization ability of active probiotic in enteron aisle simultaneously; Because Chinese medicine and active probiotic have synergy to immune system, the active probiotic that therefore will breed with Chinese medicine can strengthen the immunocompetence of poultry greatly as the poultry feed additive.We cultivate active probiotic by modern microecology principle is combined with the traditional Chinese medical science with traditional Chinese medicinal components, thereby produce a kind of antibiotic no drug residue, prophyiaxis and promoting growth, poultry feed additive with low cost of replacing fully.
Description of drawings
In order to be illustrated more clearly in the technical scheme of the embodiment of the invention, the accompanying drawing of required use was done to introduce simply during the below will describe embodiment, apparently, accompanying drawing in the following describes only is some embodiments of the present invention, for those of ordinary skill in the art, not paying under the capable prerequisite of creative work, can also obtain other accompanying drawings according to these accompanying drawings.
The schematic flow sheet of the manufacture method of the livestock fodder additives that contains the active Chinese drug component probio that Fig. 1 provides for the embodiment of the invention.
The specific embodiment
Following is clearly and completely described the technical scheme in the embodiment of the invention in conjunction with the accompanying drawing in the embodiment of the invention, and obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills belong to protection scope of the present invention not making the every other embodiment that obtains under the creative work prerequisite.
At first, need to prove that part alleged in the present specification all refers to weight portion, the below makes a detailed description to the manufacture method of the livestock fodder additives that contains the active Chinese drug component probio that the embodiment of the invention provides.
As shown in Figure 1, a kind of manufacture method that contains the livestock fodder additives of active Chinese drug component probio specifically can may further comprise the steps:
Steps A: choose the multiple poultry that equates bacterial strain quantity with the former bacterial strain of active probiotic, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, thereby obtain the primary seed solution that every kind of poultry is used active probiotic;
Wherein, described multiple poultry comprises in bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, candida utili, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds with active probiotic;
Particularly, choose accordingly the multiple poultry that the equates bacterial strain quantity former bacterial strain of active probiotic, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, can comprise with the primary seed solution of active probiotic thereby obtain every kind of poultry:
(1) with the former bacterial strain seed inclined plane inoculating of the bacillus subtilis behind the separating-purifying in the triangular flask of bacillus subtilis bacterium culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bacillus subtilis; The bacillus subtilis bacterium culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; Wherein, the pH value of this culture medium should just can be inoculated in 7.0~7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bacillus subtilis is the bacillus subtilis of the bacillus subtilis inoculation of medium 100mL of every 500mL.
And/or,
(2) with the former bacterial strain seed inclined plane inoculating of the bafillus natto behind the separating-purifying in the triangular flask of bafillus natto culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bafillus natto; The bafillus natto culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; The pH value of this culture medium should just can be inoculated in 7.0~7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bafillus natto is the bafillus natto of the bafillus natto inoculation of medium 100mL of every 500mL.
And/or,
(3) with the former bacterial strain seed inclined plane inoculating of the bacillus licheniformis behind the separating-purifying in the triangular flask of bacillus licheniformis culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bacillus licheniformis; The bacillus licheniformis culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; The pH value of this culture medium should just can be inoculated in 7.0~7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bacillus licheniformis is the bacillus licheniformis of the bacillus licheniformis inoculation of medium 100mL of every 500mL.
And/or,
(4) with the former bacterial strain seed inclined plane inoculating of the brewer's yeast behind the separating-purifying in the triangular flask of brewer's yeast culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of brewer's yeast; The brewer's yeast culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of brewer's yeast is the brewer's yeast of the brewer's yeast inoculation of medium 100mL of every 500mL.
And/or,
(5) with the former bacterial strain seed inclined plane inoculating of the candida utili behind the separating-purifying in the triangular flask of candida utili culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of candida utili; The candida utili culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of candida utili is the candida utili of the candida utili inoculation of medium 100mL of every 500mL.
And/or,
(6) with the former bacterial strain seed inclined plane inoculating of the aspergillus niger behind the separating-purifying in the triangular flask of aspergillus niger culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of aspergillus niger; The aspergillus niger culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of aspergillus niger is the aspergillus niger of the aspergillus niger inoculation of medium 100mL of every 500mL.
And/or,
(7) with the former bacterial strain seed inclined plane inoculating of the Rhizopus oryzae behind the separating-purifying in the triangular flask of Rhizopus oryzae culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of Rhizopus oryzae; The Rhizopus oryzae culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of Rhizopus oryzae is the Rhizopus oryzae of the Rhizopus oryzae inoculation of medium 100mL of every 500mL.
And/or,
(8) with the former bacterial strain seed inclined plane inoculating of the Lactobacillus casei behind the separating-purifying in the Kolle flask of Lactobacillus casei culture medium, and 37 ℃ of lower cultivations 48 hours, thereby obtain the primary seed solution of Lactobacillus casei; The Lactobacillus casei culture medium comprise 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, (molecular formula of Tween 80 is C to 1 part Tween 80 64H 124O 26English name is POLYSORBATE 80, Chinese synonym is polyoxyethylene sorbitan monooleate or emulsifying agent T80, soluble in water, ethanol, methyl alcohol and ethyl acetate, be mainly used in doing solubilizer or the emulsifying agent of parenteral solution and oral liquid, the dispersant of capsule, the emulsifying agent of ointment and matrix, the matrix of suppository or the emulsifying agent in the food industry) and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2~6.4 scope, thereby guarantees that bacterial strain can have larger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of Lactobacillus casei is the Lactobacillus casei of the Lactobacillus casei inoculation of medium 100mL of every 500mL.
And/or,
(9) with the former bacterial strain seed inclined plane inoculating of the lactobacillus acidophilus behind the separating-purifying in the Kolle flask of lactobacillus acidophilus culture medium, and 37 ℃ of lower cultivations 48 hours, thereby obtain the primary seed solution of lactobacillus acidophilus; The lactobacillus acidophilus culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2~6.4 scope, thereby guarantees that bacterial strain can have larger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of lactobacillus acidophilus is the lactobacillus acidophilus of the lactobacillus acidophilus inoculation of medium 100mL of every 500mL.
And/or,
(10) with the former bacterial strain seed inclined plane inoculating of the Bifidobacterium behind the separating-purifying in the Kolle flask of Medium of Bifidobacterium, and 37 ℃ of lower cultivations 48 hours, thereby obtain the primary seed solution of Bifidobacterium; Medium of Bifidobacterium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2~6.4 scope, thereby guarantees that bacterial strain can have larger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of Bifidobacterium is the Bifidobacterium of inoculation 100mL in the Medium of Bifidobacterium of every 500mL.
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Particularly, in actual applications, preferably from the described 10 kinds of poultry of steps A with all selecting the identical weight primary seed solution the primary seed solution of active probiotic, then mix, thereby make the livestock fodder additives that finally makes with these 10 kinds of probios.
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Wherein, corresponding seed tank culture base comprises 3%~8% brown sugar, 0.2%~0.5% potassium dihydrogen phosphate, 0.3%~0.5% yeast extract, 0.2%~0.5% peptone and the water of surplus, and each constituent content of seed tank culture base described herein is mass percent;
Particularly, in actual production was used, for many factors such as comprehensive cost of manufacture and serviceabilities, each composition of this seed tank culture base accounts for total mass ratio can select scheme in the following table 1, and the embodiment one in wherein should table is scheme most preferably:
Table 1:
Embodiment Brown sugar Potassium dihydrogen phosphate Yeast extract Peptone Water
Embodiment one ??8% ??0.22% ??0.5% ??0.3% Surplus
Embodiment two ??7% ??0.25% ??0.4% ??0.3% Surplus
Embodiment three ??5% ??0.3% ??0.3% ??0.4% Surplus
Embodiment four ??6% ??0.2% ??0.4% ??0.2% Surplus
Further, many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base accordingly, can may further comprise the steps thereby obtain many bacterial classifications mixing secondary seed solution:
Step C1: preparation seed tank culture base;
Step C2: under 115 ℃~121 ℃, to seed tank culture base sterilization 25 minutes, be cooled to again 30 ℃;
Particularly, in actual applications, preferably select under 121 ℃ the seed tank culture base was sterilized 25 minutes, can be killed fully to guarantee the harmful bacteria in the seed tank culture base.
Step C3: many bacterial classifications Hybrid TierI seed liquor is inoculated in the seed tank culture base (many bacterial classifications Hybrid TierI seed liquor that the inoculative proportion according to 3%~5% herein refers to 3 to 5 unit volumes of inoculation in the seed tank culture base of per 100 unit volumes) according to 3%~5% inoculative proportion;
Step C4: the seed tank culture base that docking finishes carries out anaerobic fermentation, and the rotating speed of installation for fermenting is 180r/min, and fermentation temperature is 32 ℃, finishes until the pH value in the seed tank culture base reaches fermentation in 4.8 o'clock, namely obtains many bacterial classifications and mixes secondary seed solution.Generally speaking, the anaerobic fermentation of seed tank culture base generally can be finished in 48~72 hours, and after fermentation was finished, the number of active probiotic can reach every milliliter of 1,500,000,000 strains.
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out herb fermenting in the Chinese medicinal liquor culture medium, thereby make the livestock fodder additives that contains the active Chinese drug component probio;
Wherein, described Chinese medicinal liquor culture medium comprises 5%~8% brown sugar, 2%~3% traditional Chinese medicinal components, 0.2%~0.3% potassium dihydrogen phosphate, 0.3%~0.5% yeast extract and the water of surplus, and each constituent content of Chinese medicinal liquor culture medium described herein is mass percent; Described traditional Chinese medicinal components is comprised of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60~80 parts, Radix Codonopsis: 60~80 parts, gynostemma pentaphylla: 60~80 parts, wilsonii: 60~80 parts,
The root of large-flowered skullcap: 50~80 parts, Radix Isatidis: 40~60 parts, the Chinese bulbul: 40~60 parts, honeysuckle: 40~60 parts,
Radix Angelicae Sinensis: 40~60 parts, hawthorn: 50~80 parts, dried orange peel: 50~80 parts, purslane: 40~60 parts,
Radix Glycyrrhizae: 30~50 parts.
Particularly, in actual production is used, for many factors such as comprehensive cost of manufacture and serviceabilities, each composition of this Chinese medicinal liquor culture medium accounts for total mass ratio can select preferred version in the following table 2, and the embodiment 1 in wherein should table is most preferred embodiment:
Table 2:
Embodiment Brown sugar Traditional Chinese medicinal components Potassium dihydrogen phosphate Yeast extract Water
Embodiment 1 ??6% ??3% ??0.2% ??0.5% Surplus
Embodiment 2 ??5% ??2% ??0.25% ??0.3% Surplus
Embodiment 3 ??7% ??2.5% ??0.25% ??0.35% Surplus
Embodiment 4 ??8% ??2.5% ??0.3% ??0.4% Surplus
Corresponding with it, the composition of corresponding traditional Chinese medicinal components, and each composition parts by weight of accounting for the traditional Chinese medicinal components total amount can select the preferred version in the following table 3, and wherein the unit in this table is weight portion, and embodiment a is most preferred embodiment:
Table 3:
The traditional Chinese medicine ingredients title Example a Example b Example c Example d
The Radix Astragali ??80 ??60 ??70 ??75
Radix Codonopsis ??80 ??75 ??70 ??60
Gynostemma pentaphylla ??80 ??60 ??70 ??75
Wilsonii ??80 ??75 ??70 ??60
The root of large-flowered skullcap ??50 ??60 ??70 ??80
Radix Isatidis ??50 ??60 ??55 ??40
The Chinese bulbul ??50 ??40 ??55 ??60
Honeysuckle ??50 ??60 ??55 ??40
Radix Angelicae Sinensis ??60 ??40 ??50 ??55
Hawthorn ??50 ??80 ??70 ??60
Dried orange peel ??60 ??50 ??60 ??70
Purslane ??50 ??60 ??55 ??40
Radix Glycyrrhizae ??50 ??30 ??40 ??45
Further, many bacterial classifications mixing secondary seed solution are inoculated into carry out herb fermenting in the Chinese medicinal liquor culture medium accordingly, can may further comprise the steps thereby make the livestock fodder additives that contains the active Chinese drug component probio:
Step D1: preparation Chinese medicinal liquor culture medium;
Step D2: under 115 ℃~121 ℃, to Chinese medicinal liquor medium sterilization 20 minutes, be cooled to again 30 ℃;
Particularly, in actual applications, preferably select under 121 ℃ Chinese medicinal liquor medium sterilization 20 minutes, can be killed fully to guarantee the harmful bacteria in the Chinese medicinal liquor culture medium.
Step D3: many bacterial classifications are mixed secondary seed solution be inoculated into (inoculative proportion according to 5%~10% herein refers to that many bacterial classifications of 5 to 10 unit volumes of Chinese medicinal liquor inoculation of medium of per 100 unit volumes mix secondary seed solution) in the Chinese medicinal liquor culture medium according to 5%~10% inoculative proportion;
Step D4: the Chinese medicinal liquor culture medium that docking finishes carries out anaerobic fermentation, the rotating speed of installation for fermenting is 180r/min, fermentation temperature is 32 ℃, finishes until the pH value in the Chinese medicinal liquor culture medium reaches fermentation in 5.0 o'clock, namely obtains to contain the livestock fodder additives of active Chinese drug component probio; Generally speaking, the anaerobic fermentation of Chinese medicinal liquor culture medium generally can be finished in 48~72 hours, and after fermentation was finished, the number of active probiotic can reach every milliliter of 2,000,000,000 strains.
In technique scheme, the effect of each composition of traditional Chinese medicinal components of the present invention is: the Radix Astragali---sweet warm in nature, the tonifying Qi and lifting yang of distinguishing the flavor of, and its active component astragalus polyose can improve immunity of organisms; Radix Codonopsis---the sweet property of distinguishing the flavor of is flat, tonifying middle-Jiao and Qi and taste, and its active component saponin can improve immunity of organisms; Gynostemma pentaphylla---toxin expelling, regulate immunologic function, the total saponin of its active component is three times of ginseng; Wilsonii---flavor is hot warm in nature, anti-stress regulate the body disorder, but its active component eleutheroside irritation cell immunity: the root of large-flowered skullcap---rush down excess fire, except damp and hot, its active component baicalin anti-inflammatory, antibiotic; Radix Isatidis---have clearing heat and detoxicating, cool blood anti-inflammatory, its active component is indigo, indigo red etc. has resisting pathogenic microbes, strengthens immunologic function; The Chinese bulbul---heat-clearing and damp-drying drug, removing pattogenic heat from the blood and toxic material from the body; Honeysuckle---clearing heat and detoxicating, antibacterial anti-inflammatory, antiviral, adjusting immunity of organism; Radix Angelicae Sinensis---it is sweet warm in nature to distinguish the flavor of, and can enrich blood, and can invigorate blood circulation again, and its active component sodium ferulate energy activating macrophage plays immunologic function; Hawthorn---promoting digestion and removing indigestion, the promoting the circulation of qi stasis of blood of faling apart, antidiarrheal dysentery; Dried orange peel---promoting the circulation of qi invigorating the spleen is whetted the appetite in the accent, and its active component volatile oil, hesperidine have the function that promotes digestive juice secretion, improve a poor appetite; Purslane---clearing heat and detoxicating, strengthening spleen and nourishing stomach, expelling parasite; Radix Glycyrrhizae---have clearing heat and detoxicating, coordinating the drug actions of a prescription, its active component glycyrrhizic acid is cell immunomodulator.The mentioned traditional Chinese medicinal components of the present invention is according to the growth characteristic of poultry and the cultivation demand in the poultry farming process, the drug matching principle of adopt monarch, minister, helping, make, it is formulated to choose respectively a few herbs that can promote poultry digestion and growth, can strengthen the poultry immunity of organisms, can improve the poultry resistance against diseases---and at first select the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii as monarch drug in a prescription, improve animal body immunity: select simultaneously the root of large-flowered skullcap, the Chinese bulbul, Radix Isatidis, honeysuckle to improve the poultry resistance against diseases; And select Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane to promote digestion and growth; Select at last the Radix Glycyrrhizae not only can be clearing heat and detoxicating and can also be in harmonious proportion all the other all medicines.This shows that traditional Chinese medicinal components provided by the present invention not only can promote the speed of growth of poultry, and can strengthen the disease resistance ability of poultry, even can also treat some bird common diseases.
Need to prove that strain culturing equipment used in the present invention and the strain culturing technique of not describing in detail all can adopt strain culturing equipment general in the prior art and strain culturing technique, no longer are described in detail in this application.Various Chinese medicines described in the present invention all can be buied in each large Chinese medicine pharmacy easily, therefore this are no longer described in detail.The former bacterial strain of various active probiotic bacterium described in the present invention all can be introduced from Chinese common micro-organisms culture presevation administrative center (CGMCC) or agriculture microorganism fungus kind preservation administrative center (ACCC), with existing state and the survival quality of guaranteeing each bacterial classification.
As seen, the enforcement of the embodiment of the invention can significantly improve the active and field planting performance in enteron aisle of bacterial strain of active probiotic in the livestock fodder additives, and make the livestock fodder additives that makes be rich in traditional Chinese medicine ingredients, the active component that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to, performance and growth promotion performance thereby the strong body that improves livestock fodder additives is cured the disease, and then replace antibiotic use fully.
The above; only for the better specific embodiment of the present invention, but protection scope of the present invention is not limited to this, anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.

Claims (4)

1. a manufacture method that contains the livestock fodder additives of active Chinese drug component probio is characterized in that, comprising:
Steps A: choose the multiple poultry that equates bacterial strain quantity with the former bacterial strain of active probiotic, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, thereby obtain the primary seed solution that every kind of poultry is used active probiotic;
Wherein, described multiple poultry comprises in bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, candida utili, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds with active probiotic;
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out herb fermenting in the Chinese medicinal liquor culture medium, thereby make the livestock fodder additives that contains the active Chinese drug component probio;
Wherein, described Chinese medicinal liquor culture medium comprises 5%~8% brown sugar, 2%~3% traditional Chinese medicinal components, 0.2%~0.3% potassium dihydrogen phosphate, 0.3%~0.5% yeast extract and the water of surplus; Described traditional Chinese medicinal components is comprised of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60~80 parts, Radix Codonopsis: 60~80 parts, gynostemma pentaphylla: 60~80 parts, wilsonii: 60~80 parts,
The root of large-flowered skullcap: 50~80 parts, Radix Isatidis: 40~60 parts, the Chinese bulbul: 40~60 parts, honeysuckle: 40~60 parts,
Radix Angelicae Sinensis: 40~60 parts, hawthorn: 50~80 parts, dried orange peel: 50~80 parts, purslane: 40~60 parts,
Radix Glycyrrhizae: 30~50 parts.
2. the manufacture method that contains the livestock fodder additives of active Chinese drug component probio according to claim 1, it is characterized in that, the described multiple poultry of the choosing equal bacterial strain quantity former bacterial strain of active probiotic, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, comprise with the primary seed solution of active probiotic thereby obtain every kind of poultry:
With the former bacterial strain seed inclined plane inoculating of the bacillus subtilis behind the separating-purifying in the triangular flask of bacillus subtilis bacterium culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bacillus subtilis; The bacillus subtilis bacterium culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the bafillus natto behind the separating-purifying in the triangular flask of bafillus natto culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bafillus natto; The bafillus natto culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the bacillus licheniformis behind the separating-purifying in the triangular flask of bacillus licheniformis culture medium, and shaken cultivation 24 hours, thus obtain the primary seed solution of bacillus licheniformis; The bacillus licheniformis culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the brewer's yeast behind the separating-purifying in the triangular flask of brewer's yeast culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of brewer's yeast; The brewer's yeast culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the candida utili behind the separating-purifying in the triangular flask of candida utili culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of candida utili; The candida utili culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the aspergillus niger behind the separating-purifying in the triangular flask of aspergillus niger culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of aspergillus niger; The aspergillus niger culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
With the former bacterial strain seed inclined plane inoculating of the Rhizopus oryzae behind the separating-purifying in the triangular flask of Rhizopus oryzae culture medium, and shaken cultivation 48 hours, thus obtain the primary seed solution of Rhizopus oryzae; The Rhizopus oryzae culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined plane inoculating of the Lactobacillus casei behind the separating-purifying in the Kolle flask of Lactobacillus casei culture medium, and 37 ℃ of lower cultivations 48 hours, thereby is obtained the primary seed solution of Lactobacillus casei; The Lactobacillus casei culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water;
And/or,
The former bacterial strain seed inclined plane inoculating of lactobacillus acidophilus behind the separating-purifying in the Kolle flask of lactobacillus acidophilus culture medium, and 37 ℃ of lower cultivations 48 hours, thereby is obtained the primary seed solution of lactobacillus acidophilus; The lactobacillus acidophilus culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water;
And/or,
The former bacterial strain seed inclined plane inoculating of Bifidobacterium behind the separating-purifying in the Kolle flask of Medium of Bifidobacterium, and 37 ℃ of lower cultivations 48 hours, thereby is obtained the primary seed solution of Bifidobacterium; Medium of Bifidobacterium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water.
3. the manufacture method that contains the livestock fodder additives of active Chinese drug component probio according to claim 1 and 2, it is characterized in that, described many bacterial classifications Hybrid TierI seed liquor is inoculated into carried out many bacterial classifications Mixed culture in the seed tank culture base, may further comprise the steps thereby obtain many bacterial classifications mixing secondary seed solution:
Step C1: preparation seed tank culture base;
Wherein, described seed tank culture base comprises 3%~8% brown sugar, 0.2%~0.5% potassium dihydrogen phosphate, 0.3%~0.5% yeast extract, 0.2%~0.5% peptone and the water of surplus;
Step C2: under 115 ℃~121 ℃, to seed tank culture base sterilization 25 minutes, be cooled to again 30 ℃;
Step C3: many bacterial classifications Hybrid TierI seed liquor is inoculated in the seed tank culture base according to 3%~5% inoculative proportion;
Step C4: the seed tank culture base that docking finishes carries out anaerobic fermentation, and the rotating speed of installation for fermenting is 180r/min, and fermentation temperature is 32 ℃, finishes until the pH value in the seed tank culture base reaches fermentation in 4.8 o'clock, namely obtains many bacterial classifications and mixes secondary seed solution.
4. the manufacture method that contains the livestock fodder additives of active Chinese drug component probio according to claim 1 and 2, it is characterized in that, described many bacterial classifications mixing secondary seed solution are inoculated into carried out herb fermenting in the Chinese medicinal liquor culture medium, may further comprise the steps thereby make the livestock fodder additives that contains the active Chinese drug component probio:
Step D1: preparation Chinese medicinal liquor culture medium;
Step D2: under 115 ℃~121 ℃, to Chinese medicinal liquor medium sterilization 20 minutes, be cooled to again 30 ℃;
Step D3: many bacterial classifications are mixed secondary seed solution be inoculated in the Chinese medicinal liquor culture medium according to 5%~10% inoculative proportion;
Step D4: the Chinese medicinal liquor culture medium that docking finishes carries out anaerobic fermentation, the rotating speed of installation for fermenting is 180r/min, fermentation temperature is 32 ℃, finishes until the pH value in the Chinese medicinal liquor culture medium reaches fermentation in 5.0 o'clock, namely obtains to contain the livestock fodder additives of active Chinese drug component probio.
CN2011102764143A 2011-09-16 2011-09-16 Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics CN102366021B (en)

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