CN102367416B - Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive - Google Patents
Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a traditional Chinese medicine probiotic solid culture medium for cow feed, a culture method and a feed additive. The traditional Chinese medicine probiotic solid culture medium for cow feed comprises the following components in parts by mass: 60-70 parts of wheat bran, 15-20 parts of bean pulp, 10-15 parts of corn flour, 2-3 parts of brown sugar, 2-3 parts of traditional Chinese medicine component, 2-4 parts of light calcium carbonate, 0.5-0.8 part of yeast extract and 0.2-0.5 part of fulvic acid. By implementing the embodiment of the invention, the activity of active probiotic strains and the colonization performance in the intestinal tract can be greatly improved; and the feed additive prepared from the traditional Chinese medicine probiotic solid culture medium is rich in traditional Chinese medicine components, and active components having pharmacological action in the traditional Chinese medicine components can be brought into full play, so that the body-strengthening & disease-treating performance and growth promotion performance of the microecological feed additive for cows can be improved, and recessive mastitis of the cows can be effectively treated and prevented, thereby completely replacing the use of antibiotics.
Description
Technical field
The present invention relates to the animal and fowl fodder field, relate in particular to Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives for a kind of ox feed.
Background technology
Bovine subclinical mastitis is a kind of common, complicated, serious disease that can cause the dairy heavy losses, and what not only have a strong impact on cow utilizes the time limit, galactopoiesis quantity and dairy quality, and is harmful to food sanitation and human health.For a long time, the cowboying family mostly adopts and prevents bovine subclinical mastitis to the antibiotic mode of the long-term feeding of milk cow.Microbiotic has kill bacteria and suppresses the effect of pathogenic microorganism, and therefore this feeding patterns has played the effect of certain prevention bovine subclinical mastitis in actual production.But, these microbiotic can enter human body along with the edible milk of people and various dairy product, this has just caused antibiotic abuse indirectly, likely cause multiple drug-induced disease, even can produce new " super resistant organism ", thereby the mankind's life and health in this feeding patterns serious threat.
For fear of or reduce the long-term microbiotic that uses in milk cow nursing process, microecological feed additive for milk cows arises at the historic moment.So-called microecological feed additive for milk cows is the active probiotic preparation that the active probiotic useful and harmless to milk cow made through special process.Microecological feed additive for milk cows adds auxiliary material as a kind of functional ox feed, realized having no side effect, had no drug resistance, prevented bovine subclinical mastitis under without pathogenic prerequisite, not only can regulate milk cow intestinal microecology balance, improve milk cow growth rate, improve efficiency of feed utilization and can suppress the harmful intestinal tract microorganism breeding, improve the milk cow health level, strengthen milk cow immunizing power.At present, in existing milk cattle cultivating, the kind of common microecological feed additive for milk cows has nearly hundred kinds, lactic acid bacteria formulation (comprising lactobacterium acidophilus, bifidus, streptococcus faecium etc.), bacillus preparation (comprising Bacillus subtilus, bacillus cereus, B.lieheniformis etc.), fungi preparation (comprising aspergillus oryzae etc.), yeast preparation (comprising cereuisiae fermentum etc.) etc.Existing ox, use in the microbial forage additive production process, mostly only use protein substance and/or the glucide substratum as active probiotic, although bacterial strain quantitatively can satisfy the demands, but the activity of most of bacterial strains is not high, field planting performance in enteron aisle a little less than, so the effect that active probiotic should be brought into play as milk cow feed is very restricted; Simultaneously, active probiotic only can strengthen the physique of milk cow to a certain extent, and the effect of its treatment and prevention bovine subclinical mastitis is not fairly obvious, and therefore, existing milk cow microbial forage additive can not replace antibiotic use fully.
Summary of the invention
The embodiment of the present invention provides Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives for a kind of ox feed, so that significantly improve bacterial strain activity and the field planting performance in enteron aisle of active probiotic, and make the fodder additives made by it be rich in traditional Chinese medicine ingredients, can also make in traditional Chinese medicine ingredients the activeconstituents that plays pharmacological action give full play to, performance and growth promotion performance thereby the strong body that improves microecological feed additive for milk cows is cured the disease, effectively treat and the prevention bovine subclinical mastitis, and then replace antibiotic use fully.
The objective of the invention is to be achieved through the following technical solutions:
Chinese medicine probiotic bacterium solid medium for a kind of ox feed, its composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid; The mass parts of its each composition is:
Wheat bran, 60～70 parts;
Dregs of beans, 15～20 parts;
Semen Maydis powder, 10～15 parts;
Brown sugar, 2～3 parts;
Traditional Chinese medicinal components, 2～3 parts;
Light calcium carbonate, 2～4 parts;
Yeast extract paste, 0.5～0.8 part;
Xanthohumic acid, 0.2～0.5 part;
Wherein, the composition of described traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Preferably, the parts by weight of each composition of corresponding traditional Chinese medicinal components are:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
Preferably, this ox feed by the mass parts of each composition of Chinese medicine probiotic bacterium solid medium is:
Wheat bran, 67.8 parts;
Dregs of beans, 15 parts;
Semen Maydis powder, 10 parts;
Brown sugar, 2 parts;
Traditional Chinese medicinal components, 2 parts;
Light calcium carbonate, 2.5 parts;
Yeast extract paste, 0.5 part;
Xanthohumic acid, 0.2 part.
The cultural method of active Chinese drug component probiotic bacterium for a kind of ox feed comprises:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium solid medium according to the ox feed described in technique scheme with Chinese medicine probiotic bacterium solid medium;
Step B: the ox feed is mixed into to the substratum mixture and stirs with Chinese medicine probiotic bacterium solid medium and water, until in the substratum mixture, the mass percent of moisture reaches 35%;
Step C: under 115 ℃～121 ℃, to substratum mixture sterilizing 20 minutes, then be cooled to 30 ℃;
Step D: the active probiotic seed liquor that is every milliliter of 2,000,000,000 strains by active probiotic concentration, the ratio that is 1:1 according to mass ratio with corn cob meal is mixed into active probiotic solid state fermentation seed;
Step e: active probiotic solid state fermentation seed is inoculated in the substratum mixture after step C processes by predetermined inoculative proportion;
Step F: the substratum mixture after inoculation is completed is put into culture vessel, and carries out sealing and fermenting under 37 ℃, when the temperature in culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, until described culture vessel temperature has been fermented while no longer rising.
Preferably, predetermined inoculative proportion is accordingly: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1:5 to 3:10.
A kind of cattle feed addictive comprises: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the ox feed described in technique scheme.
As seen from the above technical solution provided by the invention, for the ox feed that the embodiment of the present invention provides, Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives adopt tens flavor Chinese medicines to use the important component part of Chinese medicine probiotic bacterium solid medium as the ox feed, both improved the performance of the activeconstituents pharmacological action in Chinese medicine, the active probiotic that makes again the activity of its active probiotic of turning out go out higher than other culture medium culturing, and strengthened the colonization ability of probiotics in enteron aisle.To immune synergy, therefore using the active probiotic of Chinese medicine breeding as fodder additives for ox due to Chinese medicine and active probiotic, its immunological competence is considerably beyond other series products.We,, by modern microecology principle is combined with the traditional Chinese medical science, cultivate active probiotic by traditional Chinese medicinal components, thereby produce a kind of antibiotic no drug residue, prophyiaxis and promoting growth, ox Chinese medicine microecological preparation with low cost of replacing fully.
The accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, in below describing embodiment, the accompanying drawing of required use is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite that creative work is capable, can also obtain other accompanying drawings according to these accompanying drawings.
The ox feed that Fig. 1 provides for the embodiment of the present invention schematic flow sheet of the cultural method of active Chinese drug component probiotic bacterium.
Embodiment
Following is clearly and completely described the technical scheme in the embodiment of the present invention in conjunction with the accompanying drawing in the embodiment of the present invention, and obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills, not making under the creative work prerequisite the every other embodiment obtained, belong to protection scope of the present invention.
For the ox the feed below embodiment of the present invention provided, Chinese medicine probiotic bacterium solid medium, cultural method and fodder additives are described in detail.
(1) Chinese medicine probiotic bacterium solid medium for the ox feed
Chinese medicine probiotic bacterium solid medium for a kind of ox feed, its concrete composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid; The mass parts of its each composition is: wheat bran, 60～70 parts; Dregs of beans, 15～20 parts; Semen Maydis powder, 10～15 parts; Brown sugar, 2～3 parts; Traditional Chinese medicinal components, 2～3 parts; (light calcium carbonate not only claims but also claims precipitated chalk to light calcium carbonate, is the trickle calcium carbonate of the particle of settling volume between 2.4～2.8mL/g made by the chemical process method; In industrial production at home, light calcium carbonate adopts carborization to make mostly---the calcining of the raw materials such as Wingdale is generated to lime and carbonic acid gas, add again water to make lime become milk of lime, then passing into carbonic acid gas makes the milk of lime carbonization generate precipitation of calcium carbonate, finally to precipitation of calcium carbonate dewatered, drying and pulverization process get final product), 2～4 parts; Yeast extract paste, 0.5～0.8 part; Xanthohumic acid, 0.2～0.5 part.Adopt wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and the xanthohumic acid substratum as active probiotic in the present invention, these materials are not only originated abundant, with low cost, and be more suitable for cultivation, packing and the preservation of active probiotic than liquid nutrient medium, therefore more be suitable for industrialized production and utilization.
Wherein, the composition of corresponding traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Particularly, in actual production is used, for many factors such as comprehensive cost of manufacture and use propertieies, this ox feed accounts for total mass ratio with each composition of Chinese medicine probiotic bacterium solid medium can select the scheme in following table 1, and wherein this embodiment mono-in showing is scheme most preferably:
Table 1:
| Composition | Embodiment mono- | Embodiment bis- | Embodiment tri- | Embodiment tetra- |
| Wheat bran | 67.8 | 61.8 | 63.8 | 64.8 |
| Dregs of beans | 15 | 18 | 17 | 16 |
| Semen Maydis powder | 10 | 12 | 10 | 12 |
| Brown sugar | 2 | 3 | 2.5 | 2 |
| Traditional Chinese medicinal components | 2.5 | 2.5 | 2.5 | 2.5 |
| Light calcium carbonate | 2 | 2 | 3 | 2 |
| Yeast extract paste | 0.5 | 0.5 | 0.7 | 0.5 |
| Xanthohumic acid | 0.2 | 0.2 | 0.5 | 0.2 |
In above table, the composition of corresponding traditional Chinese medicinal components, and the parts by weight of each composition can select the scheme in following table 2, wherein the unit in this table is weight part, and embodiment a is scheme most preferably:
Table 2:
| The traditional Chinese medicine ingredients title | Example a | Example b | Example c | Example d |
| The Radix Astragali | 80 | 70 | 75 | 60 |
| Radix Codonopsis | 80 | 60 | 65 | 75 |
| Taraxacum | 80 | 75 | 70 | 60 |
| The root of large-flowered skullcap | 50 | 40 | 55 | 60 |
| Rhizome of chuanxiong | 20 | 15 | 15 | 10 |
| Semen Vaccariae | 50 | 40 | 55 | 60 |
| Play both sides of the street | 50 | 60 | 55 | 40 |
| Radix bupleuri | 50 | 60 | 70 | 80 |
| Radix Angelicae Sinensis | 60 | 55 | 50 | 40 |
| Motherwort Herb | 60 | 50 | 55 | 70 |
| Kelp | 120 | 110 | 100 | 90 |
| Radix Glycyrrhizae | 50 | 30 | 40 | 45 |
Further, the effect of each medicine of traditional Chinese medicinal components described in the present invention is: the Radix Astragali---sweet warm in nature, the tonifying Qi and lifting yang of distinguishing the flavor of, and its activeconstituents astragalus polysaccharides can improve immunity of organisms; Radix Codonopsis---the sweet property of distinguishing the flavor of is flat, invigorating the spleen and replenishing QI and taste, and its activeconstituents saponin can improve immunity of organisms; The flat flavor of taraxacum---property is sweet can be clearing heat and detoxicating, and dispersing swelling and dissipating binds suppresses mastitis harmful bacteria (streptococcus aureus and micrococcus scarlatinae) jointly containing the composition such as taraxasterol, choline, synanthrin and the root of large-flowered skullcap; The root of large-flowered skullcap---clearing heat and moistening dryness, the effects such as removing pattogenic heat from the blood and toxic material from the body; Rhizome of chuanxiong---blood-activating and qi-promoting, yin-nourishing, stagnation resolvation, rhizome of chuanxiong is the gas medicine in blood, allows the blood tonify without causing stagnation; Semen Vaccariae---Huo Xue Tong Longitude, the lactogenesis carbuncle that disappears, it contains triterpenoid saponin, flavonoid glycoside; Play both sides of the street---promoting blood circulation and removing blood stasis, Qu Feng Tong Network, Li Shui Tong Longitude; Radix bupleuri---bitter, cold nature, return the liver and gall warp, and easypro liver and stomach are arranged, and rises the function of sun, and anti-bacteria and anti-virus also has immunoloregulation function; Radix Angelicae Sinensis---distinguish the flavor of sweet warm in nature, can enrich blood, can invigorate blood circulation again, its activeconstituents Sodium Ferulate energy activating macrophage, play immunologic function; Motherwort Herb---promoting blood circulation to remove blood stasis, containing multiple alkaloid and organoselenium, can improve the immunizing power of body; Kelp---nourish yin and calm the liver, press down the gas dissipating bind, organic iodine content is high and be rich in logical milk factor and can improve milk production of cow; Radix Glycyrrhizae---have clearing heat and detoxicating, coordinating the actions of various ingredients in a prescription, its activeconstituents Potenlini is cell immunomodulator.The mentioned traditional Chinese medicinal components of the present invention is the growth characteristic according to milk cow, cultivation demand in the treatment of bovine subclinical mastitis and prevention mechanism and milk cattle cultivating process, adopt monarch, minister, assistant, the drug matching principle made, choose respectively and can promote milk cow digestion and growth, can strengthen the milk cow immunity of organisms, the a few herbs that can improve the milk cow resistance against diseases are formulated---at first select the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis is as monarch drug in a prescription, improve animal body immunizing power, reach the purpose of benefiting qi and nourishing blood: select taraxacum simultaneously, the root of large-flowered skullcap, radix bupleuri improves its anti-bacteria and anti-virus ability, and select rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, Motherwort Herb, kelp breast promoting blood circulation and removing blood stasis, logical and improve milk yield, finally select the Radix Glycyrrhizae not only can be clearing heat and detoxicating and can also be in harmonious proportion all the other all medicines.As can be seen here, traditional Chinese medicinal components provided by the present invention not only can promote the speed of growth of milk cow, and can strengthen the disease resistance ability of milk cow, thereby can effectively treat and prevent bovine subclinical mastitis.
(2) cultural method of active Chinese drug component probiotic bacterium for the ox feed
As shown in Figure 1, the cultural method of active Chinese drug component probiotic bacterium for a kind of ox feed specifically comprises following step:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium solid medium according to the ox feed described in technique scheme with Chinese medicine probiotic bacterium solid medium;
Step B: the ox feed is mixed into to the substratum mixture and stirs with Chinese medicine probiotic bacterium solid medium and water, until in the substratum mixture, the mass percent of moisture reaches 35%;
Step C: under 115 ℃～121 ℃, to substratum mixture sterilizing 20 minutes, then be cooled to 30 ℃;
In actual applications, preferably select under 121 ℃ substratum mixture sterilizing 20 minutes, can be killed fully with the harmful bacteria in Chinese medicine probiotic bacterium solid medium to guarantee the ox feed.
Step D: the active probiotic seed liquor that is every milliliter of 2,000,000,000 strains by active probiotic concentration, the ratio that is 1:1 according to mass ratio with corn cob meal is mixed into active probiotic solid state fermentation seed;
Wherein, corresponding corn cob meal preferably passes through Micvowave Sterilization (corresponding Micvowave Sterilization refers to heat energy killing microorganisms that microwave irradiation that proportion is 300MHz～300000MHz produces and the method for gemma.This method is applicable to the sterilizing of liquid and solid materials, and solid materials is had to desiccation) after corn cob meal, in the active probiotic solid state fermentation seed that guarantees synthesized, not have unwanted bacteria.
Step e: active probiotic solid state fermentation seed is inoculated in the substratum mixture after step C processes by predetermined inoculative proportion;
Wherein, predetermined inoculative proportion is accordingly: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1:5 to 3:10; Thereby neither waste the active probiotic seed liquor, can allow again the ox feed cultivate all good active probiotics of quantity quality with Chinese medicine probiotic bacterium solid medium.
Step F: the substratum mixture after inoculation is completed is put into culture vessel, and carries out sealing and fermenting under 37 ℃, when the temperature in culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, until described culture vessel temperature has been fermented while no longer rising; More than active probiotic bacterial strain number now reaches every milliliter of 4,500,000,000 strains.In general, sealing and fermenting is 5～7 days usually, can make the culture vessel temperature no longer increase.
Particularly, ox feed provided by the present invention with the cultural method of active Chinese drug component probiotic bacterium go for to subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum, Chan ?one or more in candiyeast, aspergillus niger, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and bifidus bacillus cultivated.
(3) cattle feed addictive made with the cultural method of active Chinese drug component probiotic bacterium by the ox feed
A kind of cattle feed addictive comprises: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the described ox feed of technique scheme.
Wherein, in corresponding cattle feed addictive, can comprise subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum, Chan ?candiyeast, aspergillus niger, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and bifidus bacillus isoreactivity probiotic bacterium.
It should be noted that, strain culturing equipment used in the present invention and the strain culturing technique of not describing in detail all can adopt strain culturing equipment general in prior art and strain culturing technique, no longer are described in detail in this application.Various Chinese medicines described in the present invention all can be buied easily in each large Chinese medicine pharmacy, therefore this are no longer described in detail.The former bacterial strain of various active probiotic bacterium described in the present invention all can be introduced from Chinese common micro-organisms culture presevation administrative center (CGMCC) or agriculture microbial strains preservation administrative center (ACCC), with existing state and the survival quality of guaranteeing each bacterial classification.
Visible, the enforcement of the embodiment of the present invention can significantly improve bacterial strain activity and the field planting performance in enteron aisle of active probiotic, and make the fodder additives made by it be rich in traditional Chinese medicine ingredients, can also make in traditional Chinese medicine ingredients the activeconstituents that plays pharmacological action give full play to, performance and growth promotion performance thereby the strong body that improves microecological feed additive for milk cows is cured the disease, effectively treat and the prevention bovine subclinical mastitis, and then replace antibiotic use fully.
The above; be only the present invention's embodiment preferably, but protection scope of the present invention is not limited to this, anyly is familiar with in technical scope that those skilled in the art disclose in the present invention; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (6)
1. a Chinese medicine probiotic bacterium solid medium for the ox feed, is characterized in that, its composition comprises: wheat bran, dregs of beans, Semen Maydis powder, brown sugar, traditional Chinese medicinal components, light calcium carbonate, yeast extract paste and xanthohumic acid; The mass parts of its each composition is:
Wheat bran, 60～70 parts;
Dregs of beans, 15～20 parts;
Semen Maydis powder, 10～15 parts;
Brown sugar, 2～3 parts;
Traditional Chinese medicinal components, 2～3 parts;
Light calcium carbonate, 2～4 parts;
Yeast extract paste, 0.5～0.8 part;
Xanthohumic acid, 0.2～0.5 part;
Wherein, the composition of described traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
2. Chinese medicine probiotic bacterium solid medium for ox feed according to claim 1, is characterized in that, the parts by weight of each composition of described traditional Chinese medicinal components are:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
3. Chinese medicine probiotic bacterium solid medium for ox feed according to claim 1 and 2, is characterized in that, this ox feed by the mass parts of each composition of Chinese medicine probiotic bacterium solid medium is:
Wheat bran, 67.8 parts;
Dregs of beans, 15 parts;
Semen Maydis powder, 10 parts;
Brown sugar, 2 parts;
Traditional Chinese medicinal components, 2 parts;
Light calcium carbonate, 2.5 parts;
Yeast extract paste, 0.5 part;
Xanthohumic acid, 0.2 part.
4. the cultural method of an ox feed use active Chinese drug component probiotic bacterium, is characterized in that, comprising:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium solid medium according to the described ox feed of any one in claims 1 to 3 with Chinese medicine probiotic bacterium solid medium;
Step B: the ox feed is mixed into to the substratum mixture and stirs with Chinese medicine probiotic bacterium solid medium and water, until in the substratum mixture, the mass percent of moisture reaches 35%;
Step C: under 115 ℃～121 ℃, to substratum mixture sterilizing 20 minutes, then be cooled to 30 ℃;
Step D: the active probiotic seed liquor that is every milliliter of 2,000,000,000 strains by active probiotic concentration, the ratio that is 1:1 according to mass ratio with corn cob meal is mixed into active probiotic solid state fermentation seed;
Step e: active probiotic solid state fermentation seed is inoculated in the substratum mixture after step C processes by predetermined inoculative proportion;
Step F: the substratum mixture after inoculation is completed is put into culture vessel, and carries out sealing and fermenting under 37 ℃, when the temperature in culture vessel reaches 40 ℃, culture vessel is overturn; Continuing fermentation, until described culture vessel temperature has been fermented while no longer rising.
5. the cultural method of active Chinese drug component probiotic bacterium for ox feed according to claim 4, is characterized in that, described predetermined inoculative proportion is: the volume ratio of active probiotic solid state fermentation seed and substratum mixture is between 1:5 to 3:10.
6. a cattle feed addictive, is characterized in that, comprising: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the described ox feed of any one in claim 4 to 5.
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| CN103652498B (en) * | 2013-12-18 | 2015-08-05 | 青岛百瑞吉生物工程有限公司 | A kind of biological forage for dairy cattle and preparation method thereof |
| CN104095149B (en) * | 2014-08-14 | 2016-06-15 | 郭庆 | A kind of preparation method preventing and treating bovine subclinical mastitis Traditional Chinese drug microecology additive |
| CN104435391A (en) * | 2014-12-29 | 2015-03-25 | 河南天纳图生物科技有限公司 | Fermentation traditional Chinese medicine for activating blood and increasing milk for livestock and preparation method thereof |
| CN104642813B (en) * | 2015-03-12 | 2017-09-29 | 如意情生物科技股份有限公司 | A kind of ruminant feed and preparation method thereof |
| CN106509409B (en) * | 2015-09-14 | 2020-10-13 | 天津昌农科技有限责任公司 | Fermented feed for dairy cows |
| CN106260542A (en) * | 2016-07-29 | 2017-01-04 | 河南德邻生物制品有限公司 | A kind of lactating sow Chinese medicine microbial fermentation preparation and preparation method thereof |
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| CN101406244A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Feedstuff additive for immunity enhancement of livestock and poultry |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
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| CN101406244A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Feedstuff additive for immunity enhancement of livestock and poultry |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
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