CN102367420B - Traditional Chinese medicine probiotic liquid culture medium used in cow forage, culturing method, and forage additive - Google Patents
Traditional Chinese medicine probiotic liquid culture medium used in cow forage, culturing method, and forage additive Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine probiotic liquid culture medium used in cow forages, a culturing method, and a forage additive. The culture medium comprises components of, by weight: 5 to 8% of brown sugar, 2 to 3% of traditional Chinese medicine components, 0.2 to 0.3% of potassium dihydrogen phosphate, 0.3 to 0.5% of yeast extract paste, and balance of water. With the implementation of the embodiment of the invention, strain activity and permanent planting performance of active probiotics in intestines can be greatly improved. The forage additive prepared with the culture medium is rich in traditional Chinese medicine components. Pharmacologic actions of the active components of the traditional Chinese medicine components can be fully performed. Therefore, body strengthening, disease treating, and growth promoting functions of the microbe forage additive used for cows are improved; and cow recessive mastitis can be effectively treated and prevented. Therefore, the forage additive can be used for completely replacing antibiotics.
Description
Technical field
The present invention relates to animal and fowl fodder field, relate in particular to Chinese medicine probiotic bacterium liquid nutrient medium, cultural method and fodder additives for a kind of ox feed.
Background technology
Bovine subclinical mastitis is a kind of serious disease common, complicated, that can cause dairy heavy losses, and what not only have a strong impact on cow utilizes the time limit, galactopoiesis quantity and dairy quality, and is harmful to food sanitation and human health.For a long time, cowboying family mostly adopts and prevents bovine subclinical mastitis to the antibiotic mode of the long-term feeding of milk cow.Microbiotic has kill bacteria and suppresses the effect of pathogenic microorganism, and therefore this feeding patterns has played the effect of certain prevention bovine subclinical mastitis in actual production.But, these microbiotic can enter human body along with the edible milk of people and various dairy product, this has just caused antibiotic abuse indirectly, likely cause multiple drug-induced disease, even can produce new " super resistant organism ", thereby the mankind's life and health in this feeding patterns serious threat.
For fear of or reduce the long-term microbiotic that uses in milk cow nursing process, microecological feed additive for milk cows arises at the historic moment.So-called microecological feed additive for milk cows is by the active probiotic preparation that the useful and harmless active probiotic of milk cow is made through special process.Microecological feed additive for milk cows adds auxiliary material as a kind of functional ox feed, realized having no side effect, had no drug resistance, prevented bovine subclinical mastitis under without pathogenic prerequisite, not only can regulate milk cow intestinal microecology balance, improve milk cow growth rate, improve efficiency of feed utilization and can suppress harmful intestinal tract microorganism breeding, improve milk cow health level, strengthen milk cow immunizing power.At present, in existing milk cattle cultivating, the kind of common microecological feed additive for milk cows has nearly hundred kinds, lactic acid bacteria formulation (comprising lactobacterium acidophilus, bifidus, streptococcus faecium etc.), bacillus preparation (comprising Bacillus subtilus, bacillus cereus, B.lieheniformis etc.), fungi preparation (comprising aspergillus oryzae etc.), yeast preparation (comprising cereuisiae fermentum etc.) etc.Existing ox, use in microbial forage additive production process, mostly only use protein substance and/or glucide as the substratum of active probiotic, although bacterial strain quantitatively can satisfy the demands, but the activity of most of bacterial strains is not high, field planting performance in enteron aisle a little less than, so the effect that active probiotic should be brought into play as milk cow feed is very restricted; Meanwhile, active probiotic only can strengthen the physique of milk cow to a certain extent, and the effect of its treatment and prevention bovine subclinical mastitis is not fairly obvious, and therefore, existing milk cow microbial forage additive can not replace antibiotic use completely.
Summary of the invention
The embodiment of the present invention provides Chinese medicine probiotic bacterium liquid nutrient medium, cultural method and fodder additives for a kind of ox feed, so that significantly improve bacterial strain activity and the field planting performance in enteron aisle of active probiotic, and make the fodder additives being made by it be rich in traditional Chinese medicine ingredients, can also make the activeconstituents that plays pharmacological action in traditional Chinese medicine ingredients give full play to, performance and growth promotion performance thereby the strong body that improves microecological feed additive for milk cows is cured the disease, effectively treat and prevention bovine subclinical mastitis, and then replace antibiotic use completely.
The object of the invention is to be achieved through the following technical solutions:
A Chinese medicine probiotic bacterium liquid nutrient medium for ox feed, its composition comprises: brown sugar, traditional Chinese medicinal components, potassium primary phosphate, yeast extract paste and water; Its each composition accounts for total mass ratio:
Brown sugar, 5%～8%;
Traditional Chinese medicinal components, 2%～3%;
Potassium primary phosphate, 0.2%～0.3%;
Yeast extract paste, 0.3%～0.5%;
Surplus is water;
Wherein, the composition of described traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Preferably, the parts by weight of each composition of corresponding traditional Chinese medicinal components are:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
Preferably, this ox feed accounts for total mass ratio with each composition of Chinese medicine probiotic bacterium liquid nutrient medium and is:
Brown sugar, 6%;
Traditional Chinese medicinal components, 3%;
Potassium primary phosphate, 0.2%;
Yeast extract paste, 0.5%;
Surplus is water.
A cultural method for active Chinese drug component probiotic bacterium for ox feed, comprising:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium liquid nutrient medium according to the ox feed described in technique scheme with Chinese medicine probiotic bacterium liquid nutrient medium;
Step B: under predetermined sterilising temp, ox feed is used to Chinese medicine probiotic bacterium liquid nutrient medium sterilizing 20 minutes, then be cooled to 30 ℃;
Step C: be the active probiotic seed liquor of every milliliter of 1,500,000,000 strains by active probiotic concentration, the ox feed being inoculated into after sterilizing by predetermined inoculative proportion is used in Chinese medicine probiotic bacterium liquid nutrient medium;
Step D: the ox feed that docking finishes active probiotic carries out anaerobically fermenting with Chinese medicine probiotic bacterium liquid nutrient medium, the rotating speed of fermentation unit is 180r/min, leavening temperature is 32 ℃, until described ox feed reaches at 5.0 o'clock by the pH value in Chinese medicine probiotic bacterium liquid nutrient medium, has fermented.
Preferably, corresponding predetermined inoculative proportion is: active probiotic seed liquor and ox feed use the volume ratio of Chinese medicine probiotic bacterium liquid nutrient medium between 1:20 to 1:10.
Preferably, corresponding predetermined inoculative proportion is: active probiotic seed liquor and ox feed are 1:20 by the volume ratio of Chinese medicine probiotic bacterium liquid nutrient medium.
Preferably, corresponding predetermined sterilising temp is at 115 ℃～121 ℃.
Preferably, corresponding predetermined sterilising temp is 121 ℃.
A cattle feed addictive, comprising: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the ox feed described in technique scheme.
As seen from the above technical solution provided by the invention, for the ox feed that the embodiment of the present invention provides, Chinese medicine probiotic bacterium liquid nutrient medium, cultural method and fodder additives adopt ten a few herbs as ox feed, to use the important component part of Chinese medicine probiotic bacterium liquid nutrient medium, both improved the performance of the activeconstituents pharmacological action in Chinese medicine, the active probiotic that makes again the activity of its active probiotic of turning out go out higher than other culture medium culturing, and strengthened the colonization ability of probiotics in enteron aisle.Because Chinese medicine and active probiotic are to immune synergy, therefore using the active probiotic of Chinese medicine breeding as fodder additives for ox, its immunological competence is considerably beyond other series products.We,, by modern microecology principle is combined with the traditional Chinese medical science, cultivate active probiotic by traditional Chinese medicinal components, thereby produce a kind of antibiotic no drug residue, prophyiaxis and promoting growth, ox Chinese medicine microecological preparation with low cost of replacing completely.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, below the accompanying drawing of required use during embodiment is described is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite that creative work is capable, can also obtain other accompanying drawings according to these accompanying drawings.
The ox feed that Fig. 1 provides for the embodiment of the present invention schematic flow sheet of the cultural method of active Chinese drug component probiotic bacterium.
Embodiment
Face, in conjunction with the accompanying drawing in the embodiment of the present invention, is clearly and completely described the technical scheme in the embodiment of the present invention below, and obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to protection scope of the present invention.
For the ox the feed below embodiment of the present invention being provided, Chinese medicine probiotic bacterium liquid nutrient medium, cultural method and fodder additives are described in detail.
(1) Chinese medicine probiotic bacterium liquid nutrient medium for ox feed
A Chinese medicine probiotic bacterium liquid nutrient medium for ox feed, its concrete composition comprises: brown sugar, traditional Chinese medicinal components, potassium primary phosphate, yeast extract paste and water; Its each composition accounts for total mass ratio: brown sugar, 5%～8%; Traditional Chinese medicinal components, 2%～3%; Potassium primary phosphate, 0.2%～0.3%, yeast extract paste, 0.3%～0.5%; Surplus is water.In the present invention, adopt a large amount of water and a small amount of brown sugar, traditional Chinese medicinal components, potassium primary phosphate and yeast extract paste as the substratum of active probiotic, therefore this is a kind of liquid nutrient medium, its each moiety source is abundant, with low cost, is very suitable for large-scale industrial production.
Wherein, the composition of corresponding traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
Particularly, in actual production is used, for many factors such as comprehensive cost of manufacture and use propertieies, this ox feed accounts for total mass ratio with each composition of Chinese medicine probiotic bacterium liquid nutrient medium can select the scheme in following table 1, and wherein this embodiment mono-in showing is scheme most preferably:
Table 1:
| Embodiment | Brown sugar | Traditional Chinese medicinal components | Potassium primary phosphate | Yeast extract paste | Water |
| Embodiment mono- | 6% | 3% | 0.2% | 0.5% | Surplus |
| Embodiment bis- | 7% | 2.5% | 0.3% | 0.3% | Surplus |
| Embodiment tri- | 5.5% | 2.5% | 0.25% | 0.4% | Surplus |
| Embodiment tetra- | 8% | 2% | 0.25% | 0.45% | Surplus |
In above table, the composition of corresponding traditional Chinese medicinal components, and the parts by weight that each composition accounts for traditional Chinese medicinal components total amount can select the scheme in following table 2, wherein the unit in this table is weight part, and embodiment a is scheme most preferably:
Table 2:
| Traditional Chinese medicine ingredients title | Example a | Example b | Example c | Example d |
| The Radix Astragali | 80 | 70 | 75 | 60 |
| Radix Codonopsis | 80 | 60 | 65 | 75 |
| Taraxacum | 80 | 75 | 70 | 60 |
| The root of large-flowered skullcap | 50 | 40 | 55 | 60 |
| Rhizome of chuanxiong | 20 | 15 | 15 | 10 |
| Semen Vaccariae | 50 | 40 | 55 | 60 |
| Play both sides of the street | 50 | 60 | 55 | 40 |
| Radix bupleuri | 50 | 60 | 70 | 80 |
| Radix Angelicae Sinensis | 60 | 55 | 50 | 40 |
| Motherwort Herb | 60 | 50 | 55 | 70 |
| Kelp | 120 | 110 | 100 | 90 |
| Radix Glycyrrhizae | 50 | 30 | 40 | 45 |
Further, the effect of each medicine of traditional Chinese medicinal components described in the present invention is: the Radix Astragali---sweet warm in nature, the tonifying Qi and lifting yang of taste, and its activeconstituents astragalus polysaccharides can improve immunity of organisms; Radix Codonopsis---the sweet property of taste is flat, invigorating the spleen and replenishing QI and taste, and its activeconstituents saponin can improve immunity of organisms; Taraxacum---the flat taste of property is sweet can be clearing heat and detoxicating, and dispersing swelling and dissipating binds suppresses mastitis harmful bacteria (streptococcus aureus and micrococcus scarlatinae) jointly containing the compositions such as taraxasterol, choline, synanthrin and the root of large-flowered skullcap; The root of large-flowered skullcap---clearing heat and moistening dryness, the effects such as removing pattogenic heat from the blood and toxic material from the body; Rhizome of chuanxiong---blood-activating and qi-promoting, yin-nourishing, stagnation resolvation, rhizome of chuanxiong is the gas medicine in blood, allows blood tonify without causing stagnation; Semen Vaccariae---Huo Xue Tong Longitude, the lactogenesis carbuncle that disappears, it contains triterpenoid saponin, flavonoid glycoside; Play both sides of the street---promoting blood circulation and removing blood stasis, Qu Feng Tong Network, Li Shui Tong Longitude; Radix bupleuri---bitter, cold nature, return liver and gall warp, has easypro liver and stomach, rises the function of sun, and anti-bacteria and anti-virus also has immunoloregulation function; Radix Angelicae Sinensis---taste is sweet warm in nature, can enrich blood, and can invigorate blood circulation again, and its activeconstituents Sodium Ferulate energy activating macrophage, plays immunologic function; Motherwort Herb---promoting blood circulation to remove blood stasis, containing multiple alkaloid and organoselenium, can improve the immunizing power of body; Kelp---nourish yin and calm the liver, press down gas dissipating bind, organic iodine content is high and be rich in logical milk factor and can improve milk production of cow; Radix Glycyrrhizae---have clearing heat and detoxicating, coordinating the actions of various ingredients in a prescription, its activeconstituents Potenlini is cell immunomodulator.The mentioned traditional Chinese medicinal components of the present invention is the growth characteristic according to milk cow, cultivation demand in the treatment of bovine subclinical mastitis and prevention mechanism and milk cattle cultivating process, adopt monarch, minister, assistant, the drug matching principle making, choose respectively and can promote milk cow digestion and growth, can strengthen milk cow immunity of organisms, the a few herbs that can improve milk cow resistance against diseases are formulated---first select the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis is as monarch drug in a prescription, improve animal body immunizing power, reach the object of benefiting qi and nourishing blood: select taraxacum simultaneously, the root of large-flowered skullcap, radix bupleuri improves its anti-bacteria and anti-virus ability, and select rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, Motherwort Herb, kelp breast promoting blood circulation and removing blood stasis, logical and improve milk yield, finally select the Radix Glycyrrhizae not only can be clearing heat and detoxicating and can also be in harmonious proportion all the other all medicines.As can be seen here, traditional Chinese medicinal components provided by the present invention not only can promote the speed of growth of milk cow, and can strengthen the disease resistance ability of milk cow, thereby can effectively treat and prevent bovine subclinical mastitis.
(2) cultural method of active Chinese drug component probiotic bacterium for ox feed
As shown in Figure 1, the cultural method of active Chinese drug component probiotic bacterium for a kind of ox feed, specifically comprises following step:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium liquid nutrient medium according to the ox feed described in technique scheme with Chinese medicine probiotic bacterium liquid nutrient medium;
Step B: under predetermined sterilising temp, ox feed is used to Chinese medicine probiotic bacterium liquid nutrient medium sterilizing 20 minutes, then be cooled to 30 ℃;
Wherein, corresponding predetermined sterilising temp can be between 115 ℃～121 ℃, but are preferably in actual applications 121 ℃, thereby guarantee that ox feed can be killed completely with the harmful bacteria in active probiotic substratum.
Step C: be the active probiotic seed liquor of every milliliter of 1,500,000,000 strains by active probiotic concentration, the ox feed being inoculated into after sterilizing by predetermined inoculative proportion is used in Chinese medicine probiotic bacterium liquid nutrient medium;
Wherein, corresponding predetermined inoculative proportion is: active probiotic seed liquor and ox feed use the volume ratio of Chinese medicine probiotic bacterium liquid nutrient medium between 1:20 to 1:10, but are preferably in actual applications 1:20; Thereby neither waste active probiotic seed liquor, can allow again ox feed cultivate all good active probiotics of quantity quality with active probiotic substratum.
Step D: the ox feed that docking finishes active probiotic carries out anaerobically fermenting with Chinese medicine probiotic bacterium liquid nutrient medium, the rotating speed of fermentation unit is 180r/min, leavening temperature is 32 ℃, until described ox feed reaches at 5.0 o'clock by the pH value in Chinese medicine probiotic bacterium liquid nutrient medium, has fermented.Active probiotic bacterial strain number now reaches every milliliter of 2,000,000,000 strains.In general, anaerobically fermenting is 48～72 hours conventionally, and its ox feed all can reach 5.0 by the pH value in active probiotic substratum.
Particularly, ox feed provided by the present invention with the cultural method of active Chinese drug component probiotic bacterium go for to subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum, Chan ?one or more in candiyeast, aspergillus niger, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and bifidus bacillus cultivate.
(3) cattle feed addictive being made with the cultural method of active Chinese drug component probiotic bacterium by ox feed
A cattle feed addictive, comprising: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the ox feed described in technique scheme.
Wherein, in corresponding cattle feed addictive, can comprise subtilis, bacillus natto, Bacillus licheniformis, cereuisiae fermentum, Chan ?candiyeast, aspergillus niger, Rhizopus oryzae, lactobacterium casei, Lactobacterium acidophilum and bifidus bacillus isoreactivity probiotic bacterium.
It should be noted that, strain culturing equipment used in the present invention and the strain culturing technique of not describing in detail all can adopt strain culturing equipment general in prior art and strain culturing technique, are no longer described in detail in this application.Various Chinese medicines described in the present invention all can be buied easily in each large Chinese medicine pharmacy, therefore this are no longer described in detail.The former bacterial strain of various active probiotic bacterium described in the present invention all can be introduced from Chinese common micro-organisms culture presevation administrative center (CGMCC) or agriculture microbial strains preservation administrative center (ACCC), to guarantee existing state and the survival quality of each bacterial classification.
Visible, the enforcement of the embodiment of the present invention can significantly improve bacterial strain activity and the field planting performance in enteron aisle of active probiotic, and make the fodder additives being made by it be rich in traditional Chinese medicine ingredients, can also make the activeconstituents that plays pharmacological action in traditional Chinese medicine ingredients give full play to, performance and growth promotion performance thereby the strong body that improves microecological feed additive for milk cows is cured the disease, effectively treat and prevention bovine subclinical mastitis, and then replace antibiotic use completely.
The above; be only the present invention's embodiment preferably, but protection scope of the present invention is not limited to this, is anyly familiar with in technical scope that those skilled in the art disclose in the present invention; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (9)
1. a Chinese medicine probiotic bacterium liquid nutrient medium for ox feed, is characterized in that, its composition comprises: brown sugar, traditional Chinese medicinal components, potassium primary phosphate, yeast extract paste and water; Its each composition accounts for total mass ratio:
Brown sugar, 5%～8%;
Traditional Chinese medicinal components, 2%～3%;
Potassium primary phosphate, 0.2%～0.3%;
Yeast extract paste, 0.3%～0.5%;
Surplus is water;
Wherein, the composition of described traditional Chinese medicinal components comprises: the Radix Astragali, Radix Codonopsis, taraxacum, the root of large-flowered skullcap, rhizome of chuanxiong, Semen Vaccariae, play both sides of the street, radix bupleuri, Radix Angelicae Sinensis, Motherwort Herb, kelp and Radix Glycyrrhizae;
The parts by weight of each composition of traditional Chinese medicinal components are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, taraxacum: 60～80 parts, the root of large-flowered skullcap: 40～60 parts,
Rhizome of chuanxiong: 10～20 parts, Semen Vaccariae: 40～60 parts, play both sides of the street: 40～60 parts, radix bupleuri: 50～80 parts,
Radix Angelicae Sinensis: 40～60 parts, Motherwort Herb: 50～80 parts, kelp: 80-120 part, Radix Glycyrrhizae: 30～50 parts.
2. Chinese medicine probiotic bacterium liquid nutrient medium for ox feed according to claim 1, is characterized in that, the parts by weight of each composition of described traditional Chinese medicinal components are:
The Radix Astragali: 80 parts, Radix Codonopsis: 80 parts, taraxacum: 80 parts, the root of large-flowered skullcap: 50 parts,
Rhizome of chuanxiong: 20 parts, Semen Vaccariae: 50 parts, play both sides of the street: 50 parts, radix bupleuri: 50 parts,
Radix Angelicae Sinensis: 60 parts, Motherwort Herb: 60 parts, kelp: 120 parts, Radix Glycyrrhizae: 50 parts.
3. Chinese medicine probiotic bacterium liquid nutrient medium for ox feed according to claim 1 and 2, is characterized in that, this ox feed accounts for total mass ratio with each composition of Chinese medicine probiotic bacterium liquid nutrient medium and is:
Brown sugar, 6%;
Traditional Chinese medicinal components, 3%;
Potassium primary phosphate, 0.2%;
Yeast extract paste, 0.5%;
Surplus is water.
4. a cultural method for active Chinese drug component probiotic bacterium for ox feed, is characterized in that, comprising:
Steps A: the moiety preparation ox feed Chinese medicine probiotic bacterium liquid nutrient medium according to the ox feed described in any one in claims 1 to 3 with Chinese medicine probiotic bacterium liquid nutrient medium;
Step B: under predetermined sterilising temp, ox feed is used to Chinese medicine probiotic bacterium liquid nutrient medium sterilizing 20 minutes, then be cooled to 30 ℃;
Step C: be the active probiotic seed liquor of every milliliter of 1,500,000,000 strains by active probiotic concentration, the ox feed being inoculated into after sterilizing by predetermined inoculative proportion is used in Chinese medicine probiotic bacterium liquid nutrient medium;
Step D: the ox feed that docking finishes active probiotic carries out anaerobically fermenting with Chinese medicine probiotic bacterium liquid nutrient medium, the rotating speed of fermentation unit is 180r/min, leavening temperature is 32 ℃, until described ox feed reaches at 5.0 o'clock by the pH value in Chinese medicine probiotic bacterium liquid nutrient medium, has fermented.
5. the cultural method of active Chinese drug component probiotic bacterium for ox feed according to claim 4, it is characterized in that, described predetermined inoculative proportion is: active probiotic seed liquor and ox feed use the volume ratio of Chinese medicine probiotic bacterium liquid nutrient medium between 1:20 to 1:10.
6. the cultural method of active Chinese drug component probiotic bacterium for ox feed according to claim 5, is characterized in that, described predetermined inoculative proportion is: active probiotic seed liquor and ox feed are 1:20 by the volume ratio of Chinese medicine probiotic bacterium liquid nutrient medium.
7. the cultural method with active Chinese drug component probiotic bacterium according to the ox feed described in claim 4,5 or 6, is characterized in that, described predetermined sterilising temp is at 115 ℃～121 ℃.
8. the cultural method of active Chinese drug component probiotic bacterium for ox feed according to claim 7, is characterized in that, described predetermined sterilising temp is 121 ℃.
9. a cattle feed addictive, is characterized in that, comprising: the active Chinese drug component probiotic bacterium of cultivating with the cultural method of active Chinese drug component probiotic bacterium according to the ox feed described in claim 4 to 8 any one.
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| CN102669482B (en) * | 2012-06-09 | 2013-05-22 | 河南中医学院 | Chinese herbal medicine feed additive for preventing cow recessive mastitis |
| CN104510779B (en) * | 2013-09-29 | 2017-12-22 | 瑞普（天津）生物药业有限公司 | A kind of Chinese medicine microecological preparation for treating cow subclinical mastitis |
| CN104095149B (en) * | 2014-08-14 | 2016-06-15 | 郭庆 | A kind of preparation method preventing and treating bovine subclinical mastitis Traditional Chinese drug microecology additive |
| CN116098938A (en) * | 2022-12-30 | 2023-05-12 | 安徽科技学院 | Treatment method for improving milk cow reproduction rate and milk yield by adopting Chinese herbal medicine preparation and application |
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| CN101406244A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Feedstuff additive for immunity enhancement of livestock and poultry |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
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