CN101653200A - Composite bioactivity feed additive and preparation method thereof - Google Patents

Composite bioactivity feed additive and preparation method thereof Download PDF

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CN101653200A
CN101653200A CN200910023852A CN200910023852A CN101653200A CN 101653200 A CN101653200 A CN 101653200A CN 200910023852 A CN200910023852 A CN 200910023852A CN 200910023852 A CN200910023852 A CN 200910023852A CN 101653200 A CN101653200 A CN 101653200A
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zeolite powder
feed additive
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CN101653200B (en
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魏军
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BAOJI BODI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention relates to the field of production and processing of livestock and poultry feed additive, and discloses a composite bioactivity feed additive and a preparation method thereof. The effective components thereof comprise saccharicterpenin, traditional Chinese components such as cyrtomium rhizome, hawthorn, large head atractylodes rhizome, astragalus root, immature bitter orange, betelnut, medicated leaven and malt, and viable bacteria components such as bacillus subtilis, bacillus licheniformis, lactobacillus planetarium, saccharomyces cerevisiae, bifidobacterium and photosyntheticbacteria; the carrier is zeolite; the above components are smashed, sieved and mixed to obtain the product.

Description

A kind of composite bioactivity feed additive and preparation method thereof
Technical field
The present invention relates to the production and the manufacture field of additive for farm animal feed, particularly a kind of composite bioactivity feed additive and preparation method thereof.
Background technology
Antibiotic application had once been made huge contribution to the development that herding is produced, feeding antibiotic can suppress harmful microbe growth and breeding in the livestock and poultry alimentary tract, reduce endotrophic consumption, save vitamin and protein etc., promote the growth of the profitable strain of the essential nutriment of synthetic animal in the alimentary canal, breeding, make the synthetic increase of nutriment, suppress or kill some pathogenic microorganism, strengthen the resistance against diseases of livestock and poultry, ward off disease, make the attenuation of intestines wall, thereby help the infiltration and the absorption of nutrition, improve a poor appetite, can stimulate the pituitary gland secreting hormone simultaneously, promote to grow.Studz (1983) report adds the quantity that Bacitracin Zinc has reduced shuttle shape bacillocin in the intestines, has played growth promoting function simultaneously.Defwang (1985) report, antibiotic is in body weight of chicken and the very big improvement on the feeding effect.Hathaway (1990) find to feed to contain in the serum of antibiotic pig and a kind ofly can promote external muscle cell breeding division, and under conditions in vitro, reducing the material of muscle protein, this effect comes from a kind of minimizing of inhibiting factor or increasing of a kind of somatomedin probably.People such as Culotta (1994) report adds antibiotic and also can improve the lean meat percentage of pig in the feed.In addition, people such as Roura find that the content of feeding cytokine in the antibiotic chicken blood flow reduces, cytokine is a kind of parahormone compound, and the effect with immune stimulatory function contacts with antigen for a long time or the long-time stimulus immune system can make the distribution of nutrient use owing to tissue growth turns to him.
But, antibiotic being applied in when bringing the very big interests of people, its negative effect has also been brought increasing trouble to the mankind: 1, the abuse of antibiotics meeting is developed immunity to drugs bacterium; 2, use antibiotic to cause the immunity degradation of livestock and poultry body for a long time; 3, use antibiotic can cause the livestock and poultry autogenous infection for a long time; 4, it is residual to use antibiotic to cause in livestock products and environment for a long time.These problems form potential threat to human health, more and more are subject to people's attention, and have issued in succession in European Union, Japanese developed country and have forbidden and limit and use the application of Antibiotic Additive in feed.World Health Organization has sent warning in the special meeting in October, 1997: the antibiotic property system that comprises four kinds of bacteriums of salmonella has passed to the mankind from animal, the mankind are being faced with and are being subjected to not and can the antibiotic aitiogenic risk of infection of curing the disease be appealed the careful antibiotic of using of livestock and poultry.Professor Xue Hengping of Agricultural University Of Nanjing once work literary composition has done detailed argumentation to antibiotic as the negative effect that feed addictive brought, and proposed gravely " in feed addictive, not use antibacterials such as medical antibiotic; no matter from recent still long-term interest; all be imperative; shoulder heavy responsibilities; expect the relevant decision-making section of China, take out practical ways and measure early.
Because people propose higher requirement to the safety issue of livestock products, this makes chemical synthesis class additive (antibiotic, hormone etc.) fall under suspicion in the status of additive agent field.We can not deny chemical synthesis class additive in the effect that improves livestock products specific yield and livestock products quantitative aspects, but in use there are many drawbacks really in it.At first, the long-term use will inevitably cause pathogenic microorganism to develop immunity to drugs, thereby dosage is constantly increased, and this has not only strengthened drug cost, has reduced culture benefit, has also increased the residual of animal drug disposition.Secondly, when killing the harmful bacterium of body, suppress inevitably or kill profitable strain in the animal body, destroyed the microbial balance in the body, influence livestock and poultry and grow normally.In addition, chemical synthetic drug residual has certain relation in human common deformity, cancer and some intoxicating phenomenon and the livestock products.Flourishing in the world country takes to forbid or limit the measure of using the chemical synthetic drug additive gradually.For example, many medicated premixs such as virginiamycin, phosphoric acid Taylor rhzomorph, spiramvcin, Bacitracin Zinc use that has been under an embargo.Seek the substitute of chemical synthesis class additive, become the feed industry urgent problem.
Summary of the invention
Problem to be solved by this invention is to seek the substitute of chemical synthesis class additive, and a kind of composite bioactivity feed additive of environmental protection is provided.
Another object of the present invention is to provide the preparation method of above-mentioned a kind of composite bioactivity feed additive.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions.
Technical scheme one: a kind of composite bioactivity feed additive, it is characterized in that, its active ingredient comprises saccharicter-penin, traditional Chinese medicinal components rhizome of cyrtomium, hawthorn, the bighead atractylodes rhizome, the Radix Astragali, the dried immature fruit of citron orange, betel nut, Divine Comedy and Fructus Hordei Germinatus, viable bacteria component bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, S. cervisiae, Bifidobacterium and photosynthetic bacteria; Carrier is a zeolite powder.
The further characteristics of technique scheme are:
Described traditional Chinese medicinal components percent by weight is 10%, viable bacteria component percent by weight is 5.25%, the saccharicter-penin percent by weight is 5%, and all the other are zeolite powder;
It is 1 part rhizome of cyrtomium, 2 parts hawthorn, 2 parts the bighead atractylodes rhizome, 2 parts the Radix Astragali, 2 parts the dried immature fruit of citron orange, 1 part betel nut, 2 parts Divine Comedy and 2 parts Fructus Hordei Germinatus that described traditional Chinese medicinal components comprises weight portion;
It is 2 parts bacillus subtilis, 1 part bacillus licheniformis, 1 part lactobacillus plantarum, 1 part S. cervisiae, 1 part Bifidobacterium and 0.25 part photosynthetic bacteria that described viable bacteria component comprises weight portion.
Technical scheme two: a kind of preparation method of composite bioactivity feed additive, it is characterized in that, may further comprise the steps:
(1) preparation traditional Chinese medicinal components: take by weighing 1 part rhizome of cyrtomium, 2 parts hawthorn, 2 parts the bighead atractylodes rhizome, 2 parts the Radix Astragali, 2 parts the dried immature fruit of citron orange, 1 part betel nut, 2 parts Divine Comedy and 2 parts Fructus Hordei Germinatus according to weight, pulverize, mix, cross 80 mesh sieves, it is stand-by to get traditional Chinese medicinal components:
(2) the preparation carrier is the viable bacteria component of zeolite powder: at first, adopt the solid agar medium to cultivate respectively bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, S. cervisiae, Bifidobacterium, photosynthetic bacteria, and scrape with physiological saline and to wash, the mixed zeolite powder dries, again after pulverizing, cross 80 mesh sieves, getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification; Then, according to carrier is the effective content of each bacterial classification of zeolite powder, and calculating and take by weighing weight portion respectively is 2 parts bacillus subtilis, 1 part bacillus licheniformis, 1 part lactobacillus plantarum, 1 part S. cervisiae, 1 part Bifidobacterium and 0.25 part photosynthetic bacteria; At last, mix, pulverize, cross 80 mesh sieves, carrier is that the viable bacteria component of zeolite powder is stand-by and calculate its effective content;
(3) at first, take by weighing traditional Chinese medicinal components 10%, effective viable bacteria component 5.25%, saccharicter-penin 5% according to percent by weight, all the other are zeolite powder; Then, mix, pulverize, cross 80 mesh sieves, promptly get composite bioactivity feed additive.
Contain traditional Chinese medicinal components among the present invention, wherein Divine Comedy: strengthening the spleen and stomach, in the accent that helps digestion; Fructus Hordei Germinatus: aid digestion, hypoglycemic, antimycotic, promoting digestion and removing indigestion; Hawthorn: appetite-stimulating indigestion-relieving, long-pending, promoting blood circulation to remove blood stasis, the promoting the circulation of qi of reducing phlegm of disappearing change to stagnate; The bighead atractylodes rhizome: strengthening the spleen and replenishing qi, eliminating dampness Li Shui, hidroschesis, antiabortive; The Radix Astragali: invigorating qi for strengthening superficies, diuresis holder poison, apocenosis, expelling pus and promoting granulation; The dried immature fruit of citron orange: the diffusing ruffian of reducing phlegm, relieving stagnant Qi disappears long-pending; Betel nut: desinsection, broken long-pending, the therapeutic method to keep the adverse qi flowing downward, Hang Shui; Its resultant effect is that flavouring deodorizing, spleen benefiting and stimulating the appetite, change stagnate and disappear long-pendingly, promote to grow, improve utilization of nutrients, improve the livestock products quality, the expelling parasite sterilization, strengthen immunity of organisms, strengthen the livestock and poultry reproductive capability, anti-stress acting on the adaptation source.
Contain useful viable bacteria component among the present invention, wherein: the subtilin that produces in (1) bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have the obvious suppression effect to the conditioned pathogen of pathogenic bacteria or autogenous infection; Bacillus subtilis can consume the free oxygen in the environment rapidly, causes the enteron aisle hypoxemia, promotes the growth of useful anaerobic bacteria, and produces organic acid such as lactic acid, reduces the enteron aisle pH value, suppresses other pathogenic bacteria growth indirectly; Bacillus subtilis can stimulate growing of animal immune organ, activates T, bone-marrow-derived lymphocyte, improves immunoglobulin (Ig) and antibody horizontal, strengthens cellular immunity and humoral immune function, improves herd immunity; Bacillus subtilis thalline self synthesizes enzymes such as AMS, protease, lipase, cellulase, in alimentary canal with animal body in digestive enzymes together play a role; Multiple B family vitamins such as bacillus subtilis energy energy synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.(2) bacillus licheniformis can promote the growth of normal physiological anaerobic bacteria in the enteron aisle, adjusts intestinal bacilli illness, recovers function of intestinal canal; Enterobacterial infection is had special efficacy, and to light-duty or heavy chordapsus, light-duty and medium-sized acute bacillary dysentery etc. all has obvious curative effects; Can produce the resistant activity material, and have unique biological and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria; Can suppress harmful bacterium breeding, make intestinal flora keep normal; Produce digestive ferment, synthetic vitamin; Strengthen immunity; Produce hydrogen peroxide.(3) lactobacillus plantarum can decompose the sugared acid of producing, and suppresses the breeding of pathogenic bacteria and spoilage organisms, and alctasin is promptly made by the Bacillus acidi lactici of living, and can treat to digest and diarrhoea.(4) Bifidobacterium can be safeguarded enteron aisle normal bacteria colony balance, suppresses growth of pathogenic bacteria, prevents constipation, diarrhea and gastrointestinal disorders etc.; Can be antitumor; Can be in enteron aisle synthetic vitamin, amino acid and raising body to the absorption of calcium ion; Can reduce cholesterol levels in the blood, prevent and treat hypertension; Can improve the anti-lactose of dairy products, improve digestibility; Can strengthen human body immune function, prevent antibiotic side effect, anti-ageing.(5) S. cervisiae can be improved the animal gastrointestinal tract micro-ecological environment, promotes the secretion of the synthetic and enzyme of microprotein, thereby promotes the digestion and the absorption of EA material.The saccharomycete culture can also be grown by the competitive pathogenic microorganism that suppresses in intestines and stomach, improves intestines wall construction and form, the harm of mould fungus inhibition toxin.Metabolite in the culture can be used as the absorption of nutrient ingredients utilization, can also bring into play antibacterial action as prebiotics.(6) photosynthetic bacteria: improve feeding environment, reduce the concentration of gases such as ammonia, hydrogen sulfide in the colony house, improve and purifying water body water quality, for aquatic products provides good living environment.
Contain saccharicter-penin (Saccharicter-penin) among the present invention, saccharicter-penin is by carbohydrate (〉=30%), the natural bioactivity substance that glycocide (〉=30%) and organic acid are formed; Being the triterpene saponin that extracts from Camellia vegetable seeds grouts and the mixture of carbohydrate, is a kind of pale brown look, ashless fine shape crystallization, can regulate and control the immune disease-resistance poison, improves breeding performonce fo animals, improve the animal product quality.
Comprehensively above-mentioned, composite bioactivity feed additive environmental protection of the present invention, traditional Chinese medicinal components have regulate the flow of vital energy help digestion, help spleen be good for the stomach, clearing heat and detoxicating, sterilization is antibiotic, invigorating blood circulation and dissolving stasis, vigorous blood follow, enhance metabolism, tonifying Qi establishing-Yang, nourishing the blood and yin, the arresting convulsion of calming the nerves, the promoting the circulation of qi of having one's ideas straightened out, expelling parasite remove long-pending effect; The probiotics viable bacteria component has effects such as microecological balance in the enteron aisle of maintenance, increase zymoprotein, enhancing immunologic function of livestock and birds, biological antagonist effect, synthetic vitamin B family and generation antibiotics material; The saccharicter-penin component has conditioning and strengthens the humoral immunity of livestock and poultry body and the function of cellular immunity, substitute antibiotic (drug residue free), suppress effects such as pathogenic microorganism growth in vivo.Three big components are incorporated into one, give full play to leading role separately, make it to regulate the enteron aisle of livestock and poultry animal comprehensively, improve the utilization rate of feed, improve the production performance of livestock and poultry etc.
The specific embodiment
Be 300kg with every jar of mixing ability of V-Mixer below, specify the preparation process of composite bioactivity feed additive of the present invention.
(1) be 10% traditional Chinese medicinal components, 5.25% viable bacteria component, 5% saccharicter-penin according to weight ratio, all the other are zeolite powder, and the traditional Chinese medicinal components of calculating is 30kg, and the viable bacteria component is 15.75kg, and saccharicter-penin is 15kg.
(2) comprising weight portion according to traditional Chinese medicinal components is 1 part rhizome of cyrtomium, 2 parts hawthorn, 2 parts the bighead atractylodes rhizome, 2 parts the Radix Astragali, 2 parts the dried immature fruit of citron orange, 1 part betel nut, 2 parts Divine Comedy and 2 parts Fructus Hordei Germinatus, calculate rhizome of cyrtomium, betel nut and be about 2.143kg respectively, hawthorn, the bighead atractylodes rhizome, the Radix Astragali, the dried immature fruit of citron orange, Divine Comedy, Fructus Hordei Germinatus are about 4.286kg respectively; Then, above-mentioned traditional Chinese medicinal components is pulverized, mixed, cross 80 mesh sieves, stand-by behind the high temperature sterilization.
(3) the preparation carrier is the viable bacteria component of zeolite powder:
A, adopt the solid agar medium to cultivate bacillus subtilis (Bacillus subtilis), nutrient solution prescription is peptone, beef extract, sodium chloride, distilled water, pH7.4; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is the careless bacillus of zeolite powder, and calculates the effective content of careless bacillus;
B, adopt the solid agar medium to cultivate bacillus licheniformis (Bacil-lus licheniformis), nutrient solution prescription is peptone, beef extract, sodium chloride, distilled water, pH7.0; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is the bacillus licheniformis of zeolite powder, and calculates the effective content of bacillus licheniformis;
C, adopt the solid agar medium to cultivate withered lactobacillus plantarum (Lactobacillus planetarium), nutrient solution prescription is peptone, beef extract, sodium chloride, distilled water, pH6.5; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification;
D, adopt the solid agar medium to cultivate S. cervisiae (Saccharomyces cerevisiac), nutrient solution prescription is that peptone, yeast extract, pancreatin are separated junket peptone, salting liquid II, distilled water, pH value 4.5; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification;
E, adopt the solid agar medium to cultivate Bifidobacterium (Bifidobacterium), nutrient solution prescription is peptone, beef extract, sodium chloride, distilled water, pH7.0; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification;
F, adopt the solid agar medium to cultivate photosynthetic bacteria (Photosynthetic Bacteria), nutrient solution prescription is peptone, beef extract, sodium chloride, distilled water, pH7.0; Scrape with physiological saline then and wash, the mixed zeolite powder dries, and after pulverizing, crosses 80 mesh sieves again, and getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification;
G, be 15.75kg according to the viable bacteria component, it is 2 parts bacillus subtilis, 1 part bacillus licheniformis, 1 part lactobacillus plantarum, 1 part S. cervisiae, 1 part Bifidobacterium and 0.25 part photosynthetic bacteria that the viable bacteria component comprises weight portion, calculates photosynthetic bacteria respectively and is about 0.629kg; Bacillus subtilis is about 5.032kg; Bacillus licheniformis, lactobacillus plantarum, S. cervisiae, Bifidobacterium are about 2.516kg respectively; According to the effective content of each bacterial classification, converting out required carrier respectively is each bacterial classification of zeolite powder then; At last, taking by weighing carrier is each bacterial classification of zeolite powder, mixes, pulverizes, and crosses 80 mesh sieves, carrier is that the viable bacteria component of zeolite powder is stand-by.
(4) take by weighing saccharicter-penin 15kg, being mixed into traditional Chinese medicinal components and carrier is the viable bacteria component of zeolite powder, and the interpolation zeolite powder is 300kg to gross weight, in V-Mixer, mix, pulverize, cross 80 mesh sieves, require the mixture homogeneity coefficient of variation (CV)≤5%, promptly get composite bioactivity feed additive.
Below in conjunction with the test example the present invention is described in further details.
Test example 1:
Select for use the porkling of the little health of same kind, same age in days, individual difference to divide two groups at random: one group is that test group, one group are control group, and the normal feed of the test group+additive of the present invention of normal feed 2 ‰ weight mixes and feeds the normal feed of control group; Control group is consistent with test group feed, management, epidemic prevention etc.; One month nursing time of test.
Result of the test such as following table:
Group Beginning counterpoise (kg/ head) End weightening finish (kg/ head) Average daily gain (g/ head) Feedstuff-meat ratio Gross profit/head
Test group ??46.5±1.50 ??72.2 ??858±48.0 ??2.71∶1 27.71 unit
Control group ??46.6±1.30 ??72.6 ??867±53.4 ??2.95∶1 0 yuan
Conclusion (of pressure testing):
1, can improve the daily gain 13.8% of piglet, reduce the material anharmonic ratio.
2, test piglet skin light hair is bright, and the state of mind is better, and the incidence of disease reduces, and saves expenses for medicine about 53%.
3, obviously reduce diarrhea rate, ight soil ammonia stink descends, and breeding environment improves.
4, the food-residue in the ight soil obviously reduces, and improves efficiency of feed utilization and reaches 13.8%.
5, high hot epidemic situation takes place in control group, and test group is not seen.Illustrate that this product is greatly improved to the immunity of pig.
Test example 2:
The test kind: Luo Man father and mother are for ages in kind of chicken 28 week.
Test period: one week of prerun, just trying for 9 weeks, the 3rd week was collected kind of an egg.
Experiment quantity: 3840
Test grouping: select for use the kind chicken of the little health of same kind, same age in days, individual difference to divide two groups at random: one group is that test group, one group are control group.
Figure G2009100238521D00101
Basal diet is formed:
Figure G2009100238521D00102
The basal diet nutritional labeling:
Figure G2009100238521D00103
Result of the test:
Figure G2009100238521D00104
Test group and control group compare:
(1) laying rate improves 4.21%;
(2) plant the egg qualification rate and improve 5.73%;
(3) incubation rate improves 7.43%;
(4) strong young rate 3.36;
(5) every kind chicken profit increases by 6.38 yuan.
Conclusion (of pressure testing):
Composite bioactivity feed additive of the present invention can improve the production performance of kind of chicken, improves laying rate; And business efficiency is better than the antibiotic Bacitracin Zinc.
The inventor is through a large amount of raising feeding experiments, the result shows: the antibiotic that composite bioactivity feed additive of the present invention combines Chinese traditional treatment theory, the saccharicter-penin of Chinese herbal medicine substitutes theory and beneficial bacterium three big elite with bacterium system bacterium theory, has really brought into play the advantage of three big systems.

Claims (5)

1, a kind of composite bioactivity feed additive, it is characterized in that, its active ingredient comprises saccharicter-penin, traditional Chinese medicinal components rhizome of cyrtomium, hawthorn, the bighead atractylodes rhizome, the Radix Astragali, the dried immature fruit of citron orange, betel nut, Divine Comedy and Fructus Hordei Germinatus, viable bacteria component bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, S. cervisiae, Bifidobacterium and photosynthetic bacteria; Carrier is a zeolite powder.
2, a kind of composite bioactivity feed additive according to claim 1 is characterized in that, described traditional Chinese medicinal components percent by weight is 10%, viable bacteria component percent by weight is 5.25%, the saccharicter-penin percent by weight is 5%, and all the other are zeolite powder;
3, a kind of composite bioactivity feed additive according to claim 1, it is characterized in that it is 1 part rhizome of cyrtomium, 2 parts hawthorn, 2 parts the bighead atractylodes rhizome, 2 parts the Radix Astragali, 2 parts the dried immature fruit of citron orange, 1 part betel nut, 2 parts Divine Comedy and 2 parts Fructus Hordei Germinatus that described traditional Chinese medicinal components comprises weight portion;
4, a kind of composite bioactivity feed additive according to claim 1, it is characterized in that it is 2 parts bacillus subtilis, 1 part bacillus licheniformis, 1 part lactobacillus plantarum, 1 part S. cervisiae, 1 part Bifidobacterium and 0.25 part photosynthetic bacteria that described viable bacteria component comprises weight portion.
5, a kind of preparation method of composite bioactivity feed additive is characterized in that, may further comprise the steps:
(1) preparation traditional Chinese medicinal components: take by weighing 1 part rhizome of cyrtomium, 2 parts hawthorn, 2 parts the bighead atractylodes rhizome, 2 parts the Radix Astragali, 2 parts the dried immature fruit of citron orange, 1 part betel nut, 2 parts Divine Comedy and 2 parts Fructus Hordei Germinatus according to weight, pulverize, mix, cross 80 mesh sieves, it is stand-by to get traditional Chinese medicinal components:
(2) the preparation carrier is the viable bacteria component of zeolite powder: at first, adopt the solid agar medium to cultivate respectively bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, S. cervisiae, Bifidobacterium, photosynthetic bacteria, and scrape with physiological saline and to wash, the mixed zeolite powder dries, again after pulverizing, cross 80 mesh sieves, getting carrier respectively is each bacterial classification of zeolite powder, and calculates the effective content of each bacterial classification; Then, according to carrier is the effective content of each bacterial classification of zeolite powder, and calculating and take by weighing weight portion respectively is 2 parts bacillus subtilis, 1 part bacillus licheniformis, 1 part lactobacillus plantarum, 1 part S. cervisiae, 1 part Bifidobacterium and 0.25 part photosynthetic bacteria; At last, mix, pulverize, cross 80 mesh sieves, carrier is that the viable bacteria component of zeolite powder is stand-by and calculate its effective content;
(3) at first, take by weighing traditional Chinese medicinal components 10%, effective viable bacteria component 5.25%, saccharicter-penin 5% according to percent by weight, all the other are zeolite powder; Then, mix, pulverize, cross 80 mesh sieves, promptly get composite bioactivity feed additive.
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