CN108085276B - Micro-ecological composite microbial inoculum for breeding pollution and serving as intestinal tract improver at the same time and application thereof - Google Patents
Micro-ecological composite microbial inoculum for breeding pollution and serving as intestinal tract improver at the same time and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention discloses a micro-ecological composite microbial agent for breeding pollution and serving as an intestinal tract improver and application thereof. Such bacilli include, but are not limited to, bacillus subtilis, bacillus stearothermophilus, bacillus licheniformis, bacillus amyloliquefaciens, bacillus pumilus, and the like. The mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 5-15% of alcaligenes faecalis, 10-20% of bacillus, 25-45% of bifidobacterium, 12-46% of lactobacillus and 8-20% of saccharomycetes. The microecological composite microbial inoculum can establish and restore the ecological balance of dominant flora in intestinal tracts, inhibit the growth of putrefying bacteria in the intestinal tracts, reduce the conversion of protein to amine and ammonia, reduce the content of the ammonia and the amine in intestines and blood, reduce harmful gases such as ammonia and the like discharged out of bodies along with excrement, improve the air quality in houses and reduce environmental pollution.
Description
Technical Field
The invention belongs to the technical field of compound microbial preparations, and particularly relates to a micro-ecological compound microbial inoculum for breeding pollution and serving as an intestinal tract improver and application thereof.
Background
Since the 90 s of the 20 th century, the development of large-scale breeding has brought about increasingly serious environmental pollution problems while solving the problems of animal product supply and driving the economic development of rural areas. The untreated livestock and poultry manure is randomly accumulated, and the sewage and manure are randomly discharged, thereby bringing a series of environmental problems. The problem of nitrogen pollution in the livestock and poultry breeding wastewater is the most prominent, and the ammonia nitrogen in the wastewater of most farms exceeds the standard, so that the nitrate content in the underground water is too high, and the eutrophication of the water body is easily caused to deteriorate the water quality. The pollution of nitrogen to water is not a local environmental pollution problem, but a problem affecting the environment of a large watershed, which harms the eutrophication degree of estuaries and offshore sea areas and also seriously damages the whole ecological environment. The problem of nitrogen pollution in livestock wastewater is a common concern at home and abroad. At present, compared with physical and chemical methods, the microbiological method has been widely accepted by people due to its incomparable advantages of high efficiency, low cost, no secondary pollution and the like in the aspect of water pollution environment treatment, and becomes the main content of attention and research in the fields of environmental science and water treatment.
The complex microbial preparation is a microbial population in which two or more kinds of microbes are cultured together and interact with each other to exhibit the greatest functional advantage. The compound microorganism preparation has practical and potential huge application, is widely applied to various fields such as agriculture, industry, medicine, environmental protection and the like, has the advantages of quick response, low investment, simple operation, no secondary pollution and the like in the field of environmental protection, and shows huge development prospect.
The using amount of the domestic environment-friendly microbial inoculum expands obviously, according to incomplete statistics, the average annual increasing rate of the using amount of the microbial inoculum in recent years reaches more than 15 percent, and the domestic market environment-friendly microbial inoculum mainly has 3 sources: one is imported abroad, the share accounts for more than 60% of the total amount, and the representatives of the manufacturers are danish novicent, foshan bivofeng (American Bioform), Youki ball university (EM bacteria) in Japan and the like; one is produced by domestic enterprises, accounts for about 30% of market share, and represents manufacturers such as Guangzhou agricultural crown (Taizi) biotechnology limited company, Guangyu biotechnology limited company and the like; and the third is the development and production of scientific research institutions in colleges and universities, which accounts for 5-10% of the total amount and mainly represents colleges and universities such as the institute of microbiology in the Chinese academy of sciences, the Qinghua university and the like. About 35 enterprises specialized in environmental protection microbial agent production in China exist, wherein over 80 percent of the microbial agents produced by the enterprises are mainly compounded by EM (effective microorganisms) introduced in Japan and related microbial agents; about 10% of enterprises and domestic research institutes jointly develop development and production of environment-friendly special microbial inoculum. At present, more enterprises are engaged in the production of the environmental-friendly microbial inoculum in China, but the production scale of the enterprises is small, about 5-50 t/year, the concentration of the industry is low, the produced microbial inoculum mainly simulates Japanese EM (effective microorganisms), and no imported microbial inoculum has pertinence on the treatment effect of wastewater. The effect of the environment-friendly special microbial inoculum developed by scientific research institutes of colleges and universities in China can be compared favorably with that of an imported microbial inoculum, but the product conversion is slow, the product is basically in a self-production and self-marketing stage, and the scale cannot be really formed.
Disclosure of Invention
The invention aims to provide a microecological compound microbial inoculum for breeding pollution and simultaneously serving as an intestinal tract improving agent and application thereof, wherein the microecological compound microbial inoculum can establish and restore the ecological balance of dominant bacterial communities in intestinal tracts, and beneficial bacterial communities generate antibacterial substances such as organic acid, hydrogen peroxide, bacteriocin and the like in the intestinal tracts, so that the growth of putrefying bacteria in the intestinal tracts is inhibited, the urease activity is reduced, the conversion of protein to amine and ammonia is reduced, the content of the ammonia and the amine in intestines and blood is reduced, harmful gases such as ammonia and the like discharged out of bodies along with excrement are reduced, the quality of air in a house is improved, and the environmental pollution is reduced.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composite microbial preparation for improving the pollution of culture and improving intestinal tract is proportionally prepared from Alcaligenes faecalis, bacillus, bifidobacterium, lactobacillus and yeast.
Such bacilli include, but are not limited to, bacillus subtilis, bacillus stearothermophilus, bacillus licheniformis, bacillus amyloliquefaciens, bacillus pumilus, and the like.
The mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 5-15% of alcaligenes faecalis, 10-20% of bacillus, 25-45% of bifidobacterium, 12-46% of lactobacillus and 8-20% of saccharomycetes.
Further preferably, the mass percentage of each microorganism in the composite microbial inoculum in the total mass of the microorganisms is as follows: 6% of alcaligenes faecalis, 10% of bacillus, 30% of bifidobacterium, 34% of lactobacillus and 20% of yeast.
Further preferably, the mass percentage of each microorganism in the composite microbial inoculum in the total mass of the microorganisms is as follows: 10% of alcaligenes faecalis, 15% of bacillus, 40% of bifidobacterium, 25% of lactobacillus and 10% of yeast.
Further preferably, the bacillus is formed by mixing bacillus subtilis and bacillus licheniformis according to the mass ratio of 1: 1.
The micro-ecological compound microbial inoculum used for breeding pollution and simultaneously used as an intestinal tract improver is applied as a feed additive or a breeding pollution repairing agent.
The microbial compound bacteria agent of the invention reduces the pollution of malodorous substances in feces and urine by firstly establishing and restoring the advantageous bacteria group and microecological balance in the intestinal tract, and because the advantageous bacteria group generates bacteriostatic substances such as organic acid, hydrogen peroxide, bacteriocin and the like in the intestinal tract, the growth of putrefying bacteria in the intestinal tract is inhibited, the urease activity is reduced, the conversion of protein to amine and ammonia is reduced, the content of ammonia and amine in the intestine and blood is reduced, thus reducing harmful gases such as ammonia and the like discharged out of the body along with feces, improving the air quality in a house and reducing the environmental pollution.
The microbial compound bacterial agent can not only produce alkaline, neutral or acidic protease, amylase and lipase, but also produce enzyme capable of degrading complex carbohydrate in plant feed. The microecological preparation can supplement beneficial flora in digestive tract and increase endogenous enzymes in organism, thereby directly participating in metabolism of nitrogenous substances, and having remarkable effect of reducing the amount of nitrogenous substances in feces.
The composite bacteria of the invention is added with alcaligenes faecalis which can be parasitized in the intestinal cavity of animals and can inhibit the growth of microorganisms such as staphylococcus aureus, pseudomonas aeruginosa, proteus vulgaris and the like, thus being harmless to the growth of animals as a feed additive, and meanwhile, the bacterial strain can also be applied to wastewater treatment, and the alcaligenes faecalis has a certain removal effect on nitrogen and phosphorus, can be used for cultivation pollution treatment and has a remarkable effect on reducing N, P pollution of livestock and poultry manure.
Adding bacillus such as: bacillus stearothermophilus, Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus and the like can not only produce alkaline, neutral or acidic protease, amylase and lipase, but also produce enzymes capable of degrading complex carbohydrates in plant feeds, and promote protein absorption in vivo, so that a large amount of nitrogen-containing substances are reduced from excrement, and pollution is reduced. The protease, the lipase and the amylase can also consume N, P which is discharged by livestock and poultry in vitro and is not completely digested, thereby reducing the culture pollution.
The added bifidobacteria and lactobacillus can establish and restore the ecological balance of dominant flora in the intestinal tract, and the beneficial flora generates antibacterial substances such as organic acid, hydrogen peroxide, bacteriocin and the like in the intestinal tract, thereby inhibiting the growth of putrefying bacteria in the intestinal tract, reducing the activity of urease, reducing the conversion of protein to amine and ammonia, reducing the content of ammonia and amine in the intestine and blood, and reducing harmful gases such as ammonia and the like discharged out of the body along with feces.
The bifidobacterium can prevent the decarboxylation of pathogenic bacteria on amino acid, reduce the concentration of the content amine and hydrogen sulfide in the intestine, further reduce the malodorous substances discharged along with feces, and protect the ecological environment.
The yeast can reduce the concentration of ammonia nitrogen in rumen, increase the concentration of non-ammonia nitrogen, especially microorganism nitrogen, in duodenum, and change the content of amino acids in duodenum, wherein the content of methionine, sarcosine, serine and threonine is increased.
The strains of the alcaligenes faecalis, the bacillus, the bifidobacterium, the lactobacillus, the microzyme and the like are all common strains in feed additives and pollution treatment in the field.
Has the advantages that:
the microecological composite microbial inoculum can be used for treating breeding pollution and can be used as an intestinal tract improving agent, the ecological balance of dominant flora in intestinal tracts can be established and recovered, the microecological composite microbial inoculum can be used as a feed additive, the microecological composite microbial inoculum can be used as an animal intestinal tract improving agent and can be used for promoting the treatment of breeding pollution, after the microecological composite microbial inoculum is used in a pig farm, the removal rate of ammonia gas in a piggery with a dry manure cleaning structure is 87.2%, and the pollution amount of N, P in pig manure pollutants is reduced by more than. The microbial compound bacteria agent is added into the chicken feed, the deodorization effect is obvious, and the ammonia removal rate reaches 79.7%.
Detailed Description
The strains described in the following examples are all commercially available, and the sources and types of the strains used in the following examples are given only as examples for better explaining the present invention, and the strains are not particularly limited.
The bacillus subtilis is specifically bacillus subtilis 1.1086 (China general microbiological culture Collection center); the bacillus licheniformis is specifically bacillus licheniformis (CICC 20085) purchased from CICC (China center for culture Collection of Industrial microorganisms); bacillus amyloliquefaciens (CICC 20164) is purchased from CICC (China center for Industrial culture Collection of microorganisms) and Alcaligenes faecalis (BNCC 186639) is purchased from Beinai biology; the Bifidobacterium is Bifidobacterium coryniformis 1.2279 (China general microbiological culture Collection center); lactobacillus (BNCC 106633) was purchased from north na organisms; the yeast is specifically Candida utilis BNCC188871 purchased from Beinanbiota. The strains are cultured under respective optimal conditions to obtain thalli, and the microecological composite microbial inoculum is obtained by compounding 5-15% of alcaligenes faecalis, 10-20% of bacillus, 25-45% of bifidobacterium, 12-46% of lactobacillus and 8-20% of saccharomycetes according to the proportion.
Example 1
A micro-ecological compound microbial inoculum used for cultivating pollution and simultaneously used as an intestinal tract improving agent comprises the following microorganisms in percentage by mass in the total mass of the microorganisms: 6% of alcaligenes faecalis, 10% of bacillus, 30% of bifidobacterium, 34% of lactobacillus and 20% of yeast. Wherein the bacillus is formed by mixing bacillus subtilis and bacillus licheniformis according to the mass ratio of 1: 1.
The composite microbial inoculum is mixed with pig feed (big feed) according to the mass ratio of 1:50 for feeding pigs, after the composite microbial inoculum is used in a pig farm, the ammonia gas removal rate of a pigsty with a dry and clear manure structure is 87.2%, and the N, P pollution amount in pig manure pollutants is reduced by 58.5% and 69.4%.
Example 2
A micro-ecological compound microbial inoculum used for cultivating pollution and simultaneously used as an intestinal tract improving agent comprises the following microorganisms in percentage by mass in the total mass of the microorganisms: the mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 10% of alcaligenes faecalis, 15% of bacillus, 40% of bifidobacterium, 25% of lactobacillus and 10% of yeast. Wherein the bacillus is formed by mixing bacillus subtilis and bacillus amyloliquefaciens according to the mass ratio of 1: 1.
The composite microbial inoculum is mixed with poultry feed (big feed) according to the mass ratio of 1:50 and is used for feeding chickens in a chicken farm, and the microbial composite microbial inoculum is added into the chicken feed, so that the deodorization effect is remarkable, and the ammonia removal rate reaches 79.7%. The N, P pollution amount in the chicken manure pollutants is respectively reduced by 45.8 percent and 57.9 percent.
Example 3
A micro-ecological compound microbial inoculum used for breeding pollution and used as an intestinal tract improving agent simultaneously, wherein the mass parts of each microorganism in the total mass of the microorganism in the compound microbial inoculum are shown in a table 1. The compound bacteria with the following numbers 1-5 are mixed with pig feed (big feed) according to the mass ratio of 1:50 for feeding pigs, and the ammonia gas removal rate of a dry manure cleaning structure after the compound bacteria agent is used is shown in table 1.
Table 1:
Claims (5)
1. a micro-ecological composite microbial inoculum for breeding pollution and simultaneously serving as an intestinal tract improver is characterized in that the composite microbial inoculum is prepared by compounding alcaligenes faecalis, bacillus, bifidobacterium, lactobacillus and saccharomycetes in proportion; the mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 5-15% of alcaligenes faecalis, 10-20% of bacillus, 25-45% of bifidobacterium, 12-46% of lactobacillus and 8-20% of saccharomycetes; the bacillus is formed by mixing bacillus subtilis and bacillus licheniformis according to the mass ratio of 1: 1.
2. The micro-ecological composite microbial inoculum for cultivating pollution and simultaneously serving as an intestinal tract improving agent according to claim 1, wherein the mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 6% of alcaligenes faecalis, 10% of bacillus, 30% of bifidobacterium, 34% of lactobacillus and 20% of yeast.
3. The micro-ecological composite microbial inoculum for cultivating pollution and simultaneously serving as an intestinal tract improving agent according to claim 1, wherein the mass percentage of each microorganism in the total mass of the microorganism in the composite microbial inoculum is as follows: 10% of alcaligenes faecalis, 15% of bacillus, 40% of bifidobacterium, 25% of lactobacillus and 10% of yeast.
4. Use of the micro-ecological complex microbial inoculum according to any one of claims 1 to 3 for breeding pollution and simultaneously serving as an intestinal tract improving agent as a breeding pollution repairing agent.
5. Use of the micro-ecological complex microbial inoculum according to any one of claims 1-3 for breeding pollution and simultaneously as an intestinal tract improving agent in the preparation of feed additives.
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Citations (5)
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| JPS63287599A (en) * | 1987-05-20 | 1988-11-24 | Komatsugawa Kakoki Kk | Method for purifying water |
| CN101341928A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive powder agent for pig and preparation technique |
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63287599A (en) * | 1987-05-20 | 1988-11-24 | Komatsugawa Kakoki Kk | Method for purifying water |
| CN101341928A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive powder agent for pig and preparation technique |
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101653200A (en) * | 2009-09-09 | 2010-02-24 | 宝鸡博迪生物科技有限责任公司 | Composite bioactivity feed additive and preparation method thereof |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
Non-Patent Citations (1)
| Title |
|---|
| 一株高效废水除臭粪产碱菌的筛选及除臭特性研究;贺琨等;《安全与环境学报》;20140825;第14卷(第4期);摘要，图1-5 * |
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