CN111302501A - Method for treating eutrophic seawater by using composite bacteria - Google Patents
Method for treating eutrophic seawater by using composite bacteria Download PDFInfo
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- CN111302501A CN111302501A CN202010103558.8A CN202010103558A CN111302501A CN 111302501 A CN111302501 A CN 111302501A CN 202010103558 A CN202010103558 A CN 202010103558A CN 111302501 A CN111302501 A CN 111302501A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a method for treating eutrophic seawater by using composite bacteria, which comprises the following steps: the preparation method comprises the following raw materials: bacillus subtilis, rhodopseudomonas palustris, acinetobacter calcoaceticus, saccharomyces cerevisiae, alcaligenes faecalis, bacillus megaterium, molasses and the balance of aqueous solution; dissolving molasses in water, adjusting pH to strong acidity, making culture medium of composite strain, and sterilizing the culture medium; sequentially inoculating various strains into the prepared composite bacterium culture medium for fermentation culture, and adjusting the pH value to slightly reduce; carrying out anaerobic fermentation treatment on the compound bacteria; and regularly detecting the quality of the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process. The invention can rapidly decompose and degrade organic matters and mineral elements in the water body, avoid forming eutrophic seawater and reduce water body pollution.
Description
Technical Field
The invention relates to the technical field of seawater treatment, in particular to a method for treating eutrophic seawater by using composite bacteria.
Background
The seawater eutrophication refers to the phenomenon of water quality pollution caused by the excessive content of N, P and other nutritive salts in the marine water body, which is essentially caused by the unbalanced input and output of the nutritive salts, so that the species distribution of the aquatic ecosystem is unbalanced, the species grow excessively, the flow of the substances and energy of the system is influenced, and the whole marine water body ecosystem is damaged.
The current treatment method for eutrophic seawater comprises the following two methods: firstly, the input of exogenous nutrient substances is controlled, and most of water eutrophication is mainly caused by the enrichment of the externally input nutrient substances in the water. If the nutrient substances input from the outside are reduced or cut off, the possibility of nutrient substance enrichment of the water body is lost; secondly, reducing the load of endogenous nutrient substances, effectively controlling the phosphorus enrichment in the lake, and adopting different methods according to different conditions, wherein the main methods comprise 1, engineering measures including excavating sediment deposits, carrying out deep aeration of a water body, injecting water for diluting, laying plastic on the surface of the sediment and the like; 2. chemical methods, this is a class of methods that includes coagulation sedimentation and algae killing with chemicals, for example, there are many cations that can effectively precipitate phosphorus from aqueous solutions, among which the most valuable are the less expensive iron, aluminum and calcium, which all precipitate with phosphate as insoluble precipitates; 3. biological measures, namely planting aquatic plants such as emergent aquatic plants, floating plants, large-scale floating plants, periphyton, planktonic algae and submerged plants, and removing nitrogen and phosphorus nutrients in the water body by utilizing the metabolic activities of nitrogen and phosphorus absorbed by the aquatic plants; 4. the microorganism adding method comprises the step of adding a proper amount of microorganisms, namely various strains, so as to accelerate the decomposition of pollutants in water and play a role in purifying water. The growth of microorganisms is greatly influenced by the environment, such as pH value, temperature, air pressure, dissolved oxygen in a water body and the like, and the activity of the composite bacteria cannot be ensured when the eutrophic seawater is treated by the existing measures, so that the treatment efficiency of the eutrophic seawater is low, and therefore, a method for treating the eutrophic seawater by the composite bacteria is designed to solve the problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for treating eutrophic seawater by using composite bacteria.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for treating eutrophic seawater by using composite bacteria comprises the following steps:
s1, preparing the following raw materials in parts by weight: 15-20 parts of bacillus subtilis, 5-10 parts of rhodopseudomonas palustris, 4-8 parts of acinetobacter calcoaceticus, 5-10 parts of saccharomyces cerevisiae, 5-10 parts of alcaligenes faecalis, 5-10 parts of bacillus megaterium, 30-50 parts of molasses and the balance of aqueous solution;
s2, dissolving molasses in water, adjusting the pH value of the molasses to be strong acid, preparing a culture medium of the compound strain, and sterilizing the culture medium;
s3, sequentially inoculating various strains in S1 into a compound bacterium culture medium prepared in S2 for fermentation culture, and adjusting the pH value to slightly reduce;
s4, carrying out anaerobic fermentation treatment on the compound bacteria in the S3 for 20-30 days;
and S5, periodically carrying out quality detection on the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process.
Preferably, the sugar content of the molasses is greater than or equal to 5%.
Preferably, the pH value of the culture medium prepared from the molasses and the water in the S2 is 5-6.
Preferably, the pH in S3 is lowered to 3-4, and the fermentation culture is in the form of aerobic fermentation, and the time of aerobic fermentation is 2-4 d.
Preferably, the fermentation temperature in S3 and S4 is controlled at 20-40 ℃.
The method for treating eutrophic seawater by using composite bacteria, which is provided by the invention, can effectively improve the activity of composite bacterial colonies by using a culture medium prepared from molasses and water and carrying out certain sterilization and disinfection treatment on the culture medium, and the bacillus subtilis, the rhodopseudomonas palustris, the acinetobacter calcoaceticus, the saccharomyces cerevisiae, the alcaligenes faecalis and the bacillus megaterium can quickly form dominant bacterial groups in a water body, so that the indexes of various pollutants in the treated water body are obviously reduced, the effect of removing the pollutants is good, the removal rate is higher, organic matters and some mineral elements rich in the water body are quickly decomposed and degraded, the normal indexes of the water body of the seawater are ensured, the formation of eutrophic seawater is avoided, and the water body pollution is reduced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example one
A method for treating eutrophic seawater by using composite bacteria comprises the following steps:
s1, preparing the following raw materials in parts by weight: 15 parts of bacillus subtilis, 5 parts of rhodopseudomonas palustris, 4 parts of acinetobacter calcoaceticus, 5 parts of saccharomyces cerevisiae, 5 parts of alcaligenes faecalis, 5 parts of bacillus megaterium, 30 parts of molasses and the balance of aqueous solution;
s2, dissolving molasses in water, adjusting the pH value of the molasses to be strong acid, preparing a culture medium of the compound strain, and sterilizing the culture medium;
s3, sequentially inoculating various strains in S1 into a compound bacterium culture medium prepared in S2 for fermentation culture, and adjusting the pH value to slightly reduce;
s4, carrying out anaerobic fermentation treatment on the compound bacteria in the S3 for 20 days;
and S5, periodically carrying out quality detection on the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process.
The sugar degree of the molasses is more than or equal to 5%, the pH value of a culture medium prepared from the molasses and water in S2 is 5, the pH value in S3 is reduced to 3, the fermentation culture is in the form of aerobic fermentation, the time of the aerobic fermentation is 2d, and the fermentation temperature in S3 and S4 is controlled at 20 ℃.
Example two
A method for treating eutrophic seawater by using composite bacteria comprises the following steps:
s1, preparing the following raw materials in parts by weight: 17 parts of bacillus subtilis, 7 parts of rhodopseudomonas palustris, 6 parts of acinetobacter calcoaceticus, 7 parts of saccharomyces cerevisiae, 7 parts of alcaligenes faecalis, 7 parts of bacillus megaterium, 40 parts of molasses and the balance of aqueous solution;
s2, dissolving molasses in water, adjusting the pH value of the molasses to be strong acid, preparing a culture medium of the compound strain, and sterilizing the culture medium;
s3, sequentially inoculating various strains in S1 into a compound bacterium culture medium prepared in S2 for fermentation culture, and adjusting the pH value to slightly reduce;
s4, carrying out anaerobic fermentation treatment on the compound bacteria in the S3 for 25 d;
and S5, periodically carrying out quality detection on the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process.
The sugar degree of the molasses is more than or equal to 5%, the pH value of a culture medium prepared from the molasses and the water in S2 is 5.5, the pH value in S3 is reduced to 3.5, the fermentation culture is in the form of aerobic fermentation, the time of the aerobic fermentation is 3d, and the fermentation temperature in S3 and S4 is controlled at 30 ℃.
EXAMPLE III
A method for treating eutrophic seawater by using composite bacteria comprises the following steps:
s1, preparing the following raw materials in parts by weight: 20 parts of bacillus subtilis, 10 parts of rhodopseudomonas palustris, 8 parts of acinetobacter calcoaceticus, 10 parts of saccharomyces cerevisiae, 10 parts of alcaligenes faecalis, 10 parts of bacillus megaterium, 50 parts of molasses and the balance of aqueous solution;
s2, dissolving molasses in water, adjusting the pH value of the molasses to be strong acid, preparing a culture medium of the compound strain, and sterilizing the culture medium;
s3, sequentially inoculating various strains in S1 into a compound bacterium culture medium prepared in S2 for fermentation culture, and adjusting the pH value to slightly reduce;
s4, carrying out anaerobic fermentation treatment on the compound bacteria in the S3 for 30 d;
and S5, periodically carrying out quality detection on the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process.
The sugar degree of the molasses is more than or equal to 5%, the pH value of a culture medium prepared from the molasses and water in S2 is 6, the pH value in S3 is reduced to 4, the fermentation culture is in the form of aerobic fermentation, the time of the aerobic fermentation is 4d, and the fermentation temperature in S3 and S4 is controlled at 40 ℃.
The composite bacteria produced in the three groups of embodiments are put in three places of the same water area, the seawater at the three places is purified, and the nitrogen and phosphorus content in the eutrophic seawater at the three places is detected, so that the following results are obtained: (the smaller the nitrogen and phosphorus content, the better the treatment effect)
| Examples | A | II | III |
| Nitrogen content (mg/L) | 0.58 | 0.32 | 0.51 |
| Phosphorus content (mg/L) | 0.065 | 0.035 | 0.054 |
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A method for treating eutrophic seawater by using composite bacteria is characterized by comprising the following steps:
s1, preparing the following raw materials in parts by weight: 15-20 parts of bacillus subtilis, 5-10 parts of rhodopseudomonas palustris, 4-8 parts of acinetobacter calcoaceticus, 5-10 parts of saccharomyces cerevisiae, 5-10 parts of alcaligenes faecalis, 5-10 parts of bacillus megaterium, 30-50 parts of molasses and the balance of aqueous solution;
s2, dissolving molasses in water, adjusting the pH value of the molasses to be strong acid, preparing a culture medium of the compound strain, and sterilizing the culture medium;
s3, sequentially inoculating various strains in S1 into a compound bacterium culture medium prepared in S2 for fermentation culture, and adjusting the pH value to slightly reduce;
s4, carrying out anaerobic fermentation treatment on the compound bacteria in the S3 for 20-30 days;
and S5, periodically carrying out quality detection on the composite bacteria, putting the bacterial colonies qualified in detection into seawater, and blowing dissolved oxygen into the seawater through an air pump in the putting process.
2. The method for treating eutrophic seawater by using composite bacteria as recited in claim 1, wherein the sugar degree of the molasses is 5% or more.
3. The method for treating eutrophic seawater by using composite bacteria as claimed in claim 1, wherein the pH of the culture medium prepared from molasses and water in S2 is 5-6.
4. The method for processing eutrophic seawater by using composite bacteria as set forth in claim 1, wherein the pH of the S3 is lowered to 3-4, and the fermentation culture is in the form of aerobic fermentation for 2-4 days.
5. The method for treating eutrophic seawater with complex bacteria as set forth in claim 1, wherein the fermentation temperature in S3 and S4 is controlled to 20-40 ℃.
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| CN202010103558.8A CN111302501A (en) | 2020-02-20 | 2020-02-20 | Method for treating eutrophic seawater by using composite bacteria |
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| CN202010103558.8A CN111302501A (en) | 2020-02-20 | 2020-02-20 | Method for treating eutrophic seawater by using composite bacteria |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY143595A (en) * | 2009-03-23 | 2011-05-31 | Univ Teknologi Mara | Biological treatment of water bodies |
| CN102557323A (en) * | 2010-10-21 | 2012-07-11 | 江苏绿科生物技术有限公司 | Biological purification method for freshwater aquaculture water environment |
| CN102732453A (en) * | 2012-05-23 | 2012-10-17 | 绍兴虞林生物科技有限公司 | Water purification microbial agent for eutrophicated water body |
| CN104045166A (en) * | 2014-06-23 | 2014-09-17 | 河南大学 | Compound microorganism bacterium agent and application thereof in eutrophic water body treatment |
| CN108085276A (en) * | 2017-12-26 | 2018-05-29 | 江苏星海生物科技有限公司 | For breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously |
| CN108996703A (en) * | 2018-07-25 | 2018-12-14 | 青岛理工大学 | Black smelly water soil and water hydrologic cycle ecological remediation system |
-
2020
- 2020-02-20 CN CN202010103558.8A patent/CN111302501A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY143595A (en) * | 2009-03-23 | 2011-05-31 | Univ Teknologi Mara | Biological treatment of water bodies |
| CN102557323A (en) * | 2010-10-21 | 2012-07-11 | 江苏绿科生物技术有限公司 | Biological purification method for freshwater aquaculture water environment |
| CN102732453A (en) * | 2012-05-23 | 2012-10-17 | 绍兴虞林生物科技有限公司 | Water purification microbial agent for eutrophicated water body |
| CN104045166A (en) * | 2014-06-23 | 2014-09-17 | 河南大学 | Compound microorganism bacterium agent and application thereof in eutrophic water body treatment |
| CN108085276A (en) * | 2017-12-26 | 2018-05-29 | 江苏星海生物科技有限公司 | For breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously |
| CN108996703A (en) * | 2018-07-25 | 2018-12-14 | 青岛理工大学 | Black smelly water soil and water hydrologic cycle ecological remediation system |
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