CN104045166A - Compound microorganism bacterium agent and application thereof in eutrophic water body treatment - Google Patents
Compound microorganism bacterium agent and application thereof in eutrophic water body treatment Download PDFInfo
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- CN104045166A CN104045166A CN201410281976.0A CN201410281976A CN104045166A CN 104045166 A CN104045166 A CN 104045166A CN 201410281976 A CN201410281976 A CN 201410281976A CN 104045166 A CN104045166 A CN 104045166A
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Abstract
The invention relates to a compound microorganism bacterium agent which consists of Rhodopseudomonas palustris bacteria liquid, Alcaligenes faecalis bacteria liquid, Acinetobacter calcoaceticus bacteria liquid and bacillus megatherium bacteria liquid according to a volume ratio of 1:(2-3):4:2, wherein the amount of viable organisms in the four kinds of bacteria liquid is 2*10<8>cfu/ml. The invention also provides application of the compound bacterium agent in eutrophic water body treatment. The water body is subjected to pollution treatment through the four composite microbes, so that polluted substances in the water body are decomposed and metabolized, pollutants in the water body are degraded by stimulating growth of indigenous microorganisms in the environment, the index of each pollutant in the treated water body is obviously reduced, the dirt removal effect is good, and the removal rate is high.
Description
technical field
The invention belongs to biological technical field, be specifically related to a kind of complex microbial inoculum and the application in eutrophication water is processed thereof.
Background technology
In recent years, water pollution and eutrophication phenomenon were very serious, and from the whole nation, in the storehouse, 27 emphasis lakes of monitoring, 20, the storehouse, lake of the bad V class of IV-water quality, up to 74%.Wherein " three lakes " (Taihu Lake, Chaohu, Dian Chi) water quality is bad V class, and main contamination index is total nitrogen, total phosphorus, chemical oxygen demand (COD) and chlorophyll a etc.Particularly in May, 2007 Taihu Lake blue-green alga bloom event, directly affect Wuxi Drinking Water for Residents safety.The environmental quality renovation of waters, earth's surface is being faced with unprecedented challenge.
In recent years, along with expanding economy and growth in the living standard, all kinds of artificial water landscapes arise at the historic moment.By typical representational artificial water scenery water quality is investigated, the result of measuring shows: III, IV, V and bad V class water account for respectively 8%, 32%, 28% and 32% of monitoring sum, wherein V and bad V class water account for 60%, and organic indicators such as being followed successively by total nitrogen, total phosphorus, chemical oxygen demand (COD) and ammonia nitrogen mainly exceeds standard.Therefore, the eutrophic removal of Man-made scenic water is also an instant major issue.In addition, the eutrophication problem of aquaculture water also can not be ignored.
At present, the domestic method for eutrophic water restoration of the ecosystem adopts submerged vegetation repair or only adopt filter-feeding fish control algae to purify water more, and conventionally compared with the sealing water body of small scale (as below 100 mu) or can Artificial Control water level, the waters such as Artificial Control current carries out ecological water processing, and for compared with the water body of big scale, this single method is difficult to obtain ideal effect.
Summary of the invention
For overcoming prior art deficiency, the object of the invention is to provide a kind of complex microbial inoculum and the application in eutrophication water is processed thereof, and in water body after treatment, pollutent index reduces obviously, removes dirt effective, and clearance is high.
For achieving the above object, the present invention adopts following technical scheme:
A kind of complex microbial inoculum, this composite fungus agent is made up of Rhodopseudomonas palustris bacterium liquid, Bacillus foecalis alkaligenes bacterium liquid, acinetobacter calcoaceticus bacterium liquid and bacillus megaterium bacterium liquid, its volume ratio is 1:2～3:4:2, and the viable bacteria amount of above-mentioned four kinds of bacterium liquid is 2 × 10
8cfu/ml.
The application of mentioned microorganism composite fungus agent in eutrophication water is processed, is specially: described complex microbial inoculum is rendered in eutrophication water, within every 7 days, thrown bacterium and process once, throwing the bacterium treatment time is 1 month; Complex microbial inoculum injected volume is the 0.05-0.2 ‰ of water body volume.Each throw bacterium process before water sampling carry out water quality analysis mensuration, according to water quality analysis monitoring result: determine that throwing continuously the bacterium treatment time is generally one month.Can be with reference to 〝 water and the tailing water analysis monitoring method 〞 third edition (chief editor of State Bureau of Environmental Protection, Beijing: China Environmental Science Press's publication, 1997, P90 – 360), regularly carry out water quality analysis to repairing water sample, the water-quality guideline of analysis comprises total nitrogen (TN) content, total phosphorus (TP) content, chemical oxygen demand (COD) (COD) and Chlorophyll-a Content (Chla).
Concrete, described eutrophication water is landscape water body, river water or aquaculture water etc.
By these four kinds of complex microorganisms of Rhodopseudomonas palustris, Bacillus foecalis alkaligenes, acinetobacter calcoaceticus and bacillus megaterium, eutrophication water is carried out to Pollution abatement, make the pollution substance in its decomposition and metabolism water body, and the growth of indigenous microorganism in the environmental stimulation pollutent of degrading in water body, thereby reach the object that maintains water ecology balance, and then control the eutrophication of water body.
Compared to the prior art, beneficial effect of the present invention:
The present invention adopts Rhodopseudomonas palustris, Bacillus foecalis alkaligenes, acinetobacter calcoaceticus and the bacillus megaterium of four kinds of difference in functionality monoids, render to continuously by a certain percentage in landscape water body, river water and the aquaculture water of eutrophication, multiple pollutant component in water body is processed simultaneously, strong adaptability, it can form very soon dominant microflora in water body, each pollutent index in water body after treatment is obviously reduced, remove dirt effective, clearance is higher.Total nitrogen in eutrophication water (TN) clearance is reached to 88.5-91.6%, total phosphorus (TP) clearance reaches 93.3-97.9%, chemical oxygen demand (COD) (COD) clearance reaches 85.8-91.0%, and the clearance of Chlorophyll-a Content (Chla) reaches 94.8-96.5%.Due to thorough to the pollutant removal in eutrophication water, make the water body of eutrophication substantially controlled, can keep for a long time Water quality, there is good economic benefit, ecological benefits and social benefit.In addition, microbial safety of the present invention is reliably nontoxic, and in the time of purifying water body, working cost is low, only need regularly add bacterium liquid, and operation, maintenance simply, are consumed energy low, are easy to promote the use of.
embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described further, but protection scope of the present invention is not limited to this.
In following embodiment, Rhodopseudomonas palustris used, Bacillus foecalis alkaligenes, acinetobacter calcoaceticus and bacillus megaterium are all purchased from Chinese agriculture microbial strains preservation administrative center (ACCC), and bacterium numbering is respectively: 10649,01705,10479 and 10010.When preparation, can first bacterial classification be activated respectively to amplification by this area routine techniques, obtain viable bacteria amount and be 2 × 10
8four kinds of bacterium liquid of cfu/ml, then mix four kinds of bacterium liquid can obtain composite fungus agent according to corresponding volume ratio.
embodiment 1
A kind of complex microbial inoculum, this composite fungus agent is made up of Rhodopseudomonas palustris bacterium liquid, Bacillus foecalis alkaligenes bacterium liquid, acinetobacter calcoaceticus bacterium liquid and bacillus megaterium bacterium liquid, and its volume ratio is 1:2:4:2, and the viable bacteria amount of above-mentioned four kinds of bacterium liquid is 2 × 10
8cfu/ml.
The application of mentioned microorganism composite fungus agent in eutrophic landscape water body is processed, be specially: described complex microbial inoculum is rendered in eutrophic landscape water body, within every 7 days, throw bacterium and process once, injected volume is 0.05 ‰ of water body volume, and throwing the bacterium treatment time is 30 days.
Landscape water body is taken from the more serious waters of zoological park internal contamination, water quality characteristic: TN 1.55mg/L, TP 0.15mg/L, COD 87.6mg/L, Chla 117.3mg/L.Throw continuously bacterium after 30 days, with reference to 〝 water and the tailing water analysis monitoring method 〞 third edition (chief editor of State Bureau of Environmental Protection, Beijing: China Environmental Science Press's publication, 1997, P90 – 360), carry out water quality analysis to repairing water sample, the water quality that records landscape water body is TN 0.13mg/L, TP 0.01mg/L, COD 12.4mg/L, Chla 4.11mg/L.In this eutrophic landscape water body, the clearance of each pollutent: TN is 91.6%, TP is 93.3%, COD is 85.8%, Chla is 96.5%.Hence one can see that, and after processing, each pollutent index obviously reduces, and clearance is higher, and after processing, the eutrophication of landscape water body is substantially controlled.
embodiment 2
A kind of complex microbial inoculum, this composite fungus agent is made up of Rhodopseudomonas palustris bacterium liquid, Bacillus foecalis alkaligenes bacterium liquid, acinetobacter calcoaceticus bacterium liquid and bacillus megaterium bacterium liquid, and its volume ratio is 1:3:4:2, and the viable bacteria amount of above-mentioned four kinds of bacterium liquid is 2 × 10
8cfu/ml.
The application of mentioned microorganism composite fungus agent in eutrophication water is processed, is specially: described complex microbial inoculum is rendered in eutrophication water, within every 7 days, thrown bacterium and process once, injected volume is 0.2 ‰ of water body volume, throws bacterium and processes 30 days.
Water body is taken from Xiaoqinghe River water, water quality characteristic: TN 7.97mg/L, TP 0.97mg/L, COD 238.7mg/L, Chla 236.7mg/L.Throw continuously bacterium after 30 days, with reference to 〝 water and the tailing water analysis monitoring method 〞 third edition (chief editor of State Bureau of Environmental Protection, Beijing: China Environmental Science Press's publication, 1997, P90 – 360), carry out water quality analysis to repairing water sample, the water quality that records water body is TN 0.91mg/L, TP 0.02mg/L, COD 21.3mg/L, Chla 12.2mg/L.In this eutrophication river water, the clearance of each pollutent: TN is 88.5%, TP is 97.9%, COD is 91.0%, Chla is 94.8%.Hence one can see that, and after processing, each pollutent index obviously reduces, and clearance is higher, and after processing, the eutrophication of river water is substantially controlled.
embodiment 3
A kind of complex microbial inoculum, this composite fungus agent is made up of Rhodopseudomonas palustris bacterium liquid, Bacillus foecalis alkaligenes bacterium liquid, acinetobacter calcoaceticus bacterium liquid and bacillus megaterium bacterium liquid, and its volume ratio is 1:3:4:2, and the viable bacteria amount of above-mentioned four kinds of bacterium liquid is 2 × 10
8cfu/ml.
The application of mentioned microorganism composite fungus agent in eutrophication water is processed, is specially: described complex microbial inoculum is rendered in eutrophication water, within every 7 days, thrown bacterium and process once, injected volume is 0.1 ‰ of water body volume, throws bacterium and processes 30 days.
Water body is taken from aquaculture water, water quality characteristic: TN 2.07mg/L, TP 0.63mg/L, COD 95.6mg/L, Chla 134.6mg/L.Throw continuously bacterium after 30 days, with reference to 〝 water and the tailing water analysis monitoring method 〞 third edition (chief editor of State Bureau of Environmental Protection, Beijing: China Environmental Science Press's publication, 1997, P90 – 360), carry out water quality analysis to repairing water sample, the water quality that records water body is TN 0.18mg/L, TP 0.03mg/L, COD 10.3mg/L, Chla 4.98mg/L.In this eutrophication aquaculture water, the clearance of each pollutent: TN is 91.3%, TP is 95.2%, COD is 89.2%, Chla is 96.3%.Hence one can see that, and after processing, each pollutent index obviously reduces, and clearance is higher, and after processing, the eutrophication of aquaculture water is substantially controlled.
Claims (3)
1. a complex microbial inoculum, it is characterized in that, this composite fungus agent is made up of Rhodopseudomonas palustris bacterium liquid, Bacillus foecalis alkaligenes bacterium liquid, acinetobacter calcoaceticus bacterium liquid and bacillus megaterium bacterium liquid, and its volume ratio is 1:2～3:4:2, and the viable bacteria amount of above-mentioned four kinds of bacterium liquid is 2 × 10
8cfu/ml.
2. the application of complex microbial inoculum in eutrophication water is processed described in claim 1, is characterized in that, described complex microbial inoculum is rendered in eutrophication water, within every 7 days, throws bacterium and processes once, and throwing the bacterium treatment time is 1 month; Complex microbial inoculum injected volume is the 0.05-0.2 ‰ of water body volume.
3. the application of complex microbial inoculum in eutrophication water is processed as claimed in claim 2, is characterized in that, described eutrophication water is landscape water body, river water or aquaculture water.
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| CN104370377B (en) * | 2014-11-15 | 2016-04-27 | 北京紫岚风生态环境科学技术研究院 | A kind of formula of biological agent for purifying eutrophication water body and using method thereof |
| CN109455828A (en) * | 2019-01-10 | 2019-03-12 | 江苏强农农业技术服务有限公司 | A kind of application method of immobilized microorganism in livestock breeding wastewater processing |
| CN111302501A (en) * | 2020-02-20 | 2020-06-19 | 广东海洋大学深圳研究院 | Method for treating eutrophic seawater by using composite bacteria |
| CN114736816A (en) * | 2021-04-30 | 2022-07-12 | 厦门百仕洁环保科技有限公司 | Salt-tolerant composite strain for water treatment and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104370377B (en) * | 2014-11-15 | 2016-04-27 | 北京紫岚风生态环境科学技术研究院 | A kind of formula of biological agent for purifying eutrophication water body and using method thereof |
| CN109455828A (en) * | 2019-01-10 | 2019-03-12 | 江苏强农农业技术服务有限公司 | A kind of application method of immobilized microorganism in livestock breeding wastewater processing |
| CN109455828B (en) * | 2019-01-10 | 2021-08-06 | 山东亿安生物工程有限公司 | Application method of immobilized microorganism in livestock and poultry breeding wastewater treatment |
| CN111302501A (en) * | 2020-02-20 | 2020-06-19 | 广东海洋大学深圳研究院 | Method for treating eutrophic seawater by using composite bacteria |
| CN114736816A (en) * | 2021-04-30 | 2022-07-12 | 厦门百仕洁环保科技有限公司 | Salt-tolerant composite strain for water treatment and application thereof |
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Application publication date: 20140917 |