CN102139964A - Method for effectively controlling algal blooms by utilizing microbes - Google Patents

Method for effectively controlling algal blooms by utilizing microbes Download PDF

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Publication number
CN102139964A
CN102139964A CN2011100219208A CN201110021920A CN102139964A CN 102139964 A CN102139964 A CN 102139964A CN 2011100219208 A CN2011100219208 A CN 2011100219208A CN 201110021920 A CN201110021920 A CN 201110021920A CN 102139964 A CN102139964 A CN 102139964A
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water
water body
mixed bacteria
bacteria liquid
volume
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CN2011100219208A
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王琳
李季
郭廷忠
童海滨
秦明周
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Henan University
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Henan University
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Abstract

The invention relates to a method for effectively controlling algal blooms by utilizing microbes, which comprises the steps: four different physiological groups of microbes which are bacillus, acinetobacter, denitrifying bacterium and photosynthetic bacterium are mixed to form a mixed bacteria culture according to the volume ratio of 1 to 3 : 1 to 4: 1.7 to 6: 1 to 5; and bacteria addition treatment is carried out for one time every seven days according to 0.05 to 0.2per mill of addition volume of the volume of a treated water body by a continuous bacteria addition method. The mixed bacteria culture can simultaneously purify and treat various pollutants in eutrophic water body, has strong adaptability, can quickly form dominant microflora in water body, lead all pollutant indexes of purified and treated eutrophic water body to be obviously reduced and have good removal effect and higher removal rate, wherein in eutrophic water body, the removal rate of TN reaches 83.1 to 84.4%, the removal rate of TP reaches 86.7 to 92.1%, the removal rate of COD reaches 76.9 to 82.9 %, and the removal rate of Chla reaches 87.2 to 90.4%. The purification method is simple to operate and has low operating cost, is safe and reliable, can maintain water quality of water body for long term and has good economic, ecological and social benefits.

Description

A kind of method of utilizing microorganism effectively to control algal bloom
Technical field
The present invention relates to a kind of method of controlling algal bloom, particularly a kind of method of utilizing microorganism effectively to control algal bloom, it is applicable to the purifying treatment of eutrophication water.
Background technology
At present, China's body eutrophication phenomenon generally takes place, and its direct result that causes is that water quality deterioration, water taste are stench unpleasant, algal bloom, species diversity descend, water supply quality frequently takes place, has a strong impact on wawter bloom, threaten human health etc.In recent years, though in stifled source, cut and to have obtained remarkable progress aspect dirty, from the eutrophication degree analyzing, each water nutrition state index is than its former still rising to some extent.As seen, prevent and treat body eutrophication by the input of controlling exogenous nitrogen, phosphorus and do not obtain the ideal effect in a short time.But this does not also mean that the control body eutrophication does not need to control the input of exogenous nutrition salt, mainly is just to be difficult to thoroughly recover because in a single day water body forms eutrophication.
Certainly, when effectively controlling exogenous pollution, nitrogen, phosphorus and the organic loading subdued in the eutrophication water could fundamentally solve body eutrophication.At present, cut down the combination that technological approaches that endogenous pollutes mainly contains physics, chemistry, biological method and these methods both at home and abroad.Dilution is washed away, physical methods such as deep layer draining, mechanical desilting, bed mud on-the-spot disposal, and cost is too high, technical sophistication, and can only play retarding action for the generation that prevents body eutrophication, fundamentally can't resolve the problem that water quality degenerates gradually.The advantage of using chemical methods such as algicide is that the clearance of nitrogen, phosphorus and organic carbon is higher, generally can reach 75~85%, and treatment effect is stable, but easily causes secondary pollution, and sludge yield is bigger, and working cost is high.Traditional biological water treatment is because characteristics such as its cost is low, non-secondary pollutions, it is present sewage work treatment process commonly used, and the general water yield of eutrophication water is huge, influence factor is many, different with traditional biological engineering of water treatment, its improvement can not be carried out in the reactor that limits and under the artificial control condition.
In recent years, the someone carries out the research that microorganism is applied to purifying of the hypertrophic water system, by microorganism to the absorbing of pollutent, the supernormal growth of control algae.The method of utilizing microorganism that algae is controlled at present is to utilize one or both bacteriums mostly, and can only degrade to certain or certain several pollutent of causing eutrophication.The innovation and creation of a kind of " utilizing the method for microbially decontaminate aquaculture water " were once disclosed on the Chinese patent communique, its application number 200310107114.8, this method is to utilize thiobacillus denitrificans, sulfuration bacterium and four kinds of microorganisms of photosynthetic bacterium in nitrobacteria, the denitrifying bacterium that aquaculture water is purified, and the clearance of total nitrogen (TN) also can only reach 79.1%.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing microorganism effectively to control algal bloom, it can overcome the deficiency of prior art, by adding the microorganism of four kinds of difference in functionality monoids, the growth of the indigenous microorganism in the environmental stimulation pollutent in the water body of degrading, recover the original self-purification capacity of water body, thereby reach the purpose of repairing water body, simultaneously, purifying treatment to different eutrophication waters is effective, the pollutants removal rate height, and, simple to operate, can not cause secondary pollution of water.
Photosynthetic bacterium, denitrifying bacterium, acinetobacter calcoaceticus and the four kinds of microorganisms of genus bacillus that provided by Beijing Voto Sky ﹠ Land Biotech Co., Ltd. are provided in the present invention, and carry out the Pollution abatement of eutrophication water as the purpose bacterium with the physiology monoid that dirty function falls in this four classes difference, make its decompose in process of production with the metabolism water body in pollution substance, and degrade pollutent in the water body of the growth of the indigenous microorganism in the environmental stimulation, keep the water aim of ecological equilibrium thereby reach, and then the eutrophication of control water body.
The inventive method comprises following processing step:
1〉be 1~3: 1~4 by volume with genus bacillus, acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium liquid bacterium liquid: 1.7~6: 1~5 mixed becomes mixed bacteria liquid;
2 〉, with step 1 mixed bacteria liquid render in the eutrophication water, injected volume is for handling 0.05~0.2 ‰ of water body volume, described eutrophic water is landscape water body or river water or aquaculture water;
3 〉, with step 1 mixed bacteria liquid, according to step 2〉injected volume, adopt the bacterium mode of throwing continuously, throwing bacterium in per 7 days handles once, the continuous throwing bacterium treatment time is according to each bacterium processing water sampling before of throwing, and measures water-quality guideline, determines according to the water quality analysis monitoring.Be generally one month.
4 〉, adopt standard method: " water and tailing water analysis monitoring method ", the State Bureau of Environmental Protection chief editor .. third edition. Beijing: the China Environmental Science Press publishes, 1997.P90 360, regularly carry out water quality analysis, analyze water-quality guideline and be respectively total nitrogen content (TN), total phosphorous (TP), chemical oxygen demand (COD) (COD) and chlorophyll a content (Chla) repairing water sample.
The present invention mainly is the microorganism-genus bacillus of adopting four kinds of difference in functionality monoids, acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium by volume 1~3: 1~4: 1.7~6: 1~5 mixed becomes mixed bacteria liquid, and by 0.05~0.2 ‰ injected volume of processed water body volume, throw bacterium continuously, throwing bacterium in per 7 days handles once, landscape water body to eutrophication, river water, multiple pollutant component in the aquaculture water is handled simultaneously, adaptability is strong, in water body, can very fast formation dominant microflora, make that each pollutent index obviously reduces in the water after the processing, the removal dirt is effective, clearance is higher, total nitrogen content in the eutrophication water (TN) clearance is reached 83.1 84.4%, total phosphorous (TP) clearance reaches 86.7 92.1%, chemical oxygen demand (COD) (COD) clearance reaches 76.9 82.9%, the clearance of chlorophyll a content (Chla) reaches 87.2 90.4%, because it is thorough to the pollutant removal in the eutrophication water, make the water body of eutrophication controlled substantially, can keep the water body quality for a long time, have good economic benefit, ecological benefits and social benefit.And the microbial safety that the inventive method adopted is reliably nontoxic, and running cost is low when purifying water body, only need regularly add bacterium liquid, operation easily, safeguard simple, power consumption is low, be easy to promote the use of.
Embodiment
Describe the specific embodiment of the present invention in detail below in conjunction with embodiment:
Embodiment 1,
Processing to eutrophic landscape water body.Four kinds of microorganism genus bacillus that employing is provided by Beijing Voto Sky ﹠ Land Biotech Co., Ltd., acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium liquid bacterium liquid, by their volume ratio 1: 1: 2: 1 mixed becomes mixed bacteria liquid, and is standby.The test water body is taken from the more serious waters of zoological park internal contamination, water quality characteristic: COD87.6mg/L, TN 1.55mg/L, TP 0.15mg/L, Chla117.3mg/L, above-mentioned standby mixed bacteria liquid is rendered in the landscape water body of described eutrophication, injected volume is 0.05 ‰ of this water body volume, adopt the mode of throwing in bacterium liquid continuously, throwing bacterium in per 7 days handles once, throwing in mixed bacteria liquid continuously handled after 30 days, the water quality that records landscape water body is COD 20.2mg/L, TN0.25mg/L, TP 0.02mg/L, Chla 11.3mg/L, and employing standard method: " water and tailing water analysis monitoring method ", the State Bureau of Environmental Protection chief editor. the third edition. Beijing: the China Environmental Science Press publishes, 1997.P90 360, regularly analyze repairing water sample, hence one can see that, and the eutrophication of the eutrophic landscape water body after the processing is controlled substantially.To the clearance of this each pollutent of eutrophic landscape water body be that COD is 76.9% respectively, TN is 83.9%, TP is 86.7%, Chla is 90.4%.As seen, handle each pollutent index of back and obviously reduce, and clearance is higher.
Embodiment 2:
Processing to the eutrophication river water.Four kinds of microorganism genus bacillus that employing is provided by Beijing Voto Sky ﹠ Land Biotech Co., Ltd., acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium liquid bacterium liquid, by their volume ratio 1: 1: 5: 3 mixed becomes mixed bacteria liquid, and is standby.Get Xiaoqinghe River water as the test water body, water quality characteristic is COD 238.7mg/L, TN 7.97mg/L, TP 0.97mg/L, Chla 236.7mg/L, above-mentioned standby mixed bacteria liquid is rendered in the river water of described eutrophication, injected volume is 0.2 ‰ of this water body volume, adopt the mode of throwing in mixed bacteria liquid continuously, throwing in mixed bacteria liquid in per 7 days handles once, throwing in mixed bacteria liquid continuously handled after 30 days, the water quality that records water body is COD 40.7mg/L, TN 1.56mg/L, TP " " 0.08mg/L, Chla 30..3mg/L, and employing standard method: " water and tailing water analysis monitoring method ", the State Bureau of Environmental Protection chief editor. the third edition. Beijing: the China Environmental Science Press publishes, 1997.P90 360, regularly analyze repairing water sample.Draw thus, the eutrophication of the eutrophication river water after the processing is controlled substantially.To the clearance of this each pollutent of eutrophication river water be that COD is 82.9% respectively, TN is 84.4%, TP is 91.8%, Chla is 87.2%.As seen, handle each pollutent index of back and obviously reduce, and clearance is higher.
Embodiment 3:
Processing to the eutrophication aquaculture water.Four kinds of microorganism genus bacillus that employing is provided by Beijing Voto Sky ﹠ Land Biotech Co., Ltd., acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium liquid bacterium liquid, by their volume ratio 1: 1: 3: 1 mixed becomes mixed bacteria liquid, and is standby.Get and culture water as the test water body, water quality characteristic: COD 95.6mg/L, TN 2.07mg/L, TP 0.63mg/L, Chla134.6mg/L, above-mentioned standby mixed bacteria liquid is rendered in the aquaculture water of described eutrophication, injected volume is 0.1 ‰ of this water body volume, adopt the mode of throwing in mixed bacteria liquid continuously, throwing in mixed bacteria liquid in per 7 days handles once, throwing in mixed bacteria liquid continuously handled after 30 days, the water quality that records water body is COD 20.0mg/L, TN 0.35mg/L, TP0.05mg/L, Chla 13.3mg/L, and employing standard method: " water and tailing water analysis monitoring method ", the State Bureau of Environmental Protection chief editor. the third edition. Beijing: the China Environmental Science Press publishes, 1997.P90-360, regularly analyze repairing water sample, draw thus, the eutrophication of the eutrophication aquaculture water after the processing is controlled substantially, and to the clearance of this each pollutent of eutrophication aquaculture water be respectively: COD is 79.1%, TN is 83.1%, TP is 92.1%, Chla is 90.1%.As seen, handle each pollutent index of back and obviously reduce, and clearance is higher.

Claims (1)

1. method of utilizing microorganism effectively to control algal bloom is characterized in that this method comprises following processing step:
1〉with genus bacillus, acinetobacter calcoaceticus, denitrifying bacterium, photosynthetic bacterium liquid bacterium liquid by volume 1~3: 1~4: 1.7~6: 1~5 mixed becomes mixed bacteria liquid;
2〉with step 1〉mixed bacteria liquid render in the eutrophication water, injected volume is for handling 0.05~0.2 ‰ of water body volume;
3〉with step 1〉mixed bacteria liquid, according to step 2〉injected volume, mode with continuous input mixed bacteria liquid, throwing in mixed bacteria liquid in per 7 days handles once, before handling, each input mixed bacteria liquid takes a sample, measures water-quality guideline, and according to water quality analysis monitoring situation, definite input mixed bacteria liquid treatment time continuously;
4〉adopt standard method: " water and tailing water analysis monitoring method ", regularly carry out water quality analysis to repairing water sample, analyze total nitrogen content TN, total phosphorous TP, chemical oxygen demand (COD) C0D and the chlorophyll a content Chla of water quality.
CN2011100219208A 2011-01-20 2011-01-20 Method for effectively controlling algal blooms by utilizing microbes Pending CN102139964A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103103140A (en) * 2011-11-09 2013-05-15 浙江商达环保有限公司 Aerobic phosphorus removing bacterial stain for sewage treatment and application thereof
CN104045166A (en) * 2014-06-23 2014-09-17 河南大学 Compound microorganism bacterium agent and application thereof in eutrophic water body treatment
CN104370377B (en) * 2014-11-15 2016-04-27 北京紫岚风生态环境科学技术研究院 A kind of formula of biological agent for purifying eutrophication water body and using method thereof
CN111018095A (en) * 2019-12-26 2020-04-17 中国水产科学研究院渔业机械仪器研究所 Method for controlling cyanobacterial microcystis bloom and portable treatment system

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103103140A (en) * 2011-11-09 2013-05-15 浙江商达环保有限公司 Aerobic phosphorus removing bacterial stain for sewage treatment and application thereof
CN104045166A (en) * 2014-06-23 2014-09-17 河南大学 Compound microorganism bacterium agent and application thereof in eutrophic water body treatment
CN104370377B (en) * 2014-11-15 2016-04-27 北京紫岚风生态环境科学技术研究院 A kind of formula of biological agent for purifying eutrophication water body and using method thereof
CN111018095A (en) * 2019-12-26 2020-04-17 中国水产科学研究院渔业机械仪器研究所 Method for controlling cyanobacterial microcystis bloom and portable treatment system
CN111018095B (en) * 2019-12-26 2024-03-12 中国水产科学研究院渔业机械仪器研究所 Method for controlling cyanobacteria microcystis bloom and portable treatment system

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Application publication date: 20110803