CN106399200A - Alcaligenes sp. and application thereof to high-salt high-polymer waste water - Google Patents
Alcaligenes sp. and application thereof to high-salt high-polymer waste water Download PDFInfo
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- CN106399200A CN106399200A CN201610979936.2A CN201610979936A CN106399200A CN 106399200 A CN106399200 A CN 106399200A CN 201610979936 A CN201610979936 A CN 201610979936A CN 106399200 A CN106399200 A CN 106399200A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/05—Alcaligenes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a strain of alcaligenes sp. and application thereof to high-salt high-polymer waste water. The collection number of the alcaligenes sp. Z-1 provided by the invention is CGMCC No.12585. The alcaligenes sp. can effectively decompose macromolecular polymers, such as polyacrylamide, polysulfonate substances and the like, into micromolecular substances easy to degrade under the environment of high-salt high-polymer waste water, and can obtain energy by decomposing the organisms to maintain growth and reproduction. In the high-salt high-polymer waste water which contains the organisms, such as polyacrylamide, polysulfonate substances and the like, and is treated by the bacteria, the decomposition rate of the high polymer can reach above 95%, the COD (chemical oxygen demand) of the waste water can be effectively reduced and the COD removal rate can reach above 50%.
Description
Technical field
The invention belongs to microorganism and field of waste water treatment, relate to a kind of Alcaligenes Z-1 and its in the high poly- waste water of high salt
In application.
Background technology
China's oil and chemical industry produce substantial amounts of high salt organic wastewater with difficult degradation thereby, every year in these organic wastewaters often
Macromolecule polyalcohol additive containing a large amount of synthetic, these polymer molecular weight big structures complexity are it is difficult to degrade, in ring
In border, long, the harm to environment and people is larger for existence time.Such direct discharging of waste water can be to surface water, subsoil water, soil, plant
Thing and ecological environment cause severe contamination, and the survival and development to enterprise cause many negative effects, also become society, the public
The problem paying high attention to government department.
Zhang Shuxia, what shining outstanding person waits《Composite bacteria agent capable processes the research of deep-well sulphonated polymer mud waste water technology》In be found that micro- life
Thing composite bacteria agent capable HBS5, and for processing the optimum condition of difficult degradation polysulfonate class drilling fluid waste water be：Temperature more than 20 DEG C, bacterium
Agent dosage 0.5g/L (SV30 is 25%～35%), aeration rate 0.25L/m in, initial well-drilling waste water COD mass concentration≤
1100mg/L.
Although report more both at home and abroad to the wastewater treatment of the larger molecular organicses such as the material of class containing polysulfonate, polyacrylamide,
But it is 15000～38000us/cm for conductance, COD is that the used water difficult to degradate of 3500～7000mg/L is imitated with microbial treatments
Fruit less desirable always, so find one or more can conductance be 15000～38000us/cm high-salt wastewater in
Survival, and can efficient degradation sulphur class material, the strain of polyacrylamide.
Content of the invention
Inventing technical problem to be solved is to overcome the deficiencies in the prior art, provides one kind can be in high salt high polycyclic border
The middle bacterial strain that can effectively decompose the macromolecule polyalcohols such as polyacrylamide, polysulfonate class material.
The present invention is achieved by the following technical programs：
The invention provides a kind of bacterial strain Z-1, it is Alcaligenes (Alcaligenes through molecular biology identification
Sp.), this bacterial strain with 2016-6-2 day be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as
CGMCC), address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation registration number
CGMCC NO.12585.
Present invention also offers described Alcaligenes (Alcaligenes sp.) Z-1 is in the high poly- wastewater treatment of high salt
Application, in wherein said high-salt wastewater, dissolved oxygen amount is 2.0～7.0mg/L.
In application of the present invention, the inorganic salt in the high poly- waste water of described high salt is NaCl, CaCl2、MgCl2；Electricity
Lead as 15000～38000us/cm；Described high-salt wastewater COD is 5000～7000mg/L, and polymer provides COD to account for total COD
Ratio is 1%～20%.
Growth promoter is added, described production accelerator is selected from monosaccharide, disaccharide, polysaccharide in described high salt high-COD waste water
One or more of which.The COD that the dosage of preferably described growth promoter provides is former high salt high-COD waste water COD
5 ‰ -5%.
Described Alcaligenes (Alcaligenes sp.) Z-1 adds in the high poly- waste water of high salt, described composite bacteria agent
Amount/organic waste the water yield is preferably 0.1wt ‰～2wt ‰.Organic substance in certain time aeration, energy degrading waste water, reduces
The COD of waste water.
The present invention provide Alcaligenes Z-1 compared with prior art, this equal can be by larger molecular organicses effectively decomposition
For the material of the easy degraded of small molecule, and by decomposing organic matter acquisition energy, using monosaccharide in waste water, small molecular organic acid or
Multiple small organic molecule such as person's alcohols, maintains the growth and breeding of itself.Contain polypropylene through Alcaligenes Z-1 process of the present invention
In the high-salt wastewaters of Organic substance such as amide, polysulfonate class, the high polymer rate of being decomposed can reach more than 95%, can effectively reduce waste water
COD, COD clearance can reach more than 50%.The high poly- waste water of high salt is processed using Alcaligenes Z-1 of the present invention, can high degree
Reduce cost for wastewater treatment, reduce energy consumption and the material consumption of such wastewater treatment；And the increasing with strain Alcaligenes Z-1 addition
Plus, degradation efficiency is greatly improved, thus improving biochemical efficiency, reduces biochemical floor space.Additionally, working as biochemical system
After being impacted, add strain Z-1, biochemical system recovery time can be shortened.
Specific embodiment
With reference to example, technical solution of the present invention is described in further detail.Following examples are merely to illustrate this
Bright, it does not limit the scope of the invention.
The preparation method of embodiment 1 microbial bacterial agent and the application in producing polyacrylamide factory waste water
The bacterial strain Z-1 of the present invention is to be obtained by domestication culture, screening and separation from well-drilling waste water reservoir bed mud.
1.Z-1 biological characteristicses
Z-1 is monococcus, gram negative bacteria.On solid medium, bacterium colony is rounded, white, smooth, and moistening have light
Pool, neat in edge, do not glue.
2. couple bacterial strain Z-1 16S rDNA identifies, comprises the following steps that：
Forward primer F1：5 '-AGAGTTTGATCCTGGCTCAG-3 ', reverse primer R1：5’-
GGTTACCTTGTTACGACTT-3 ', carries out DNA cloning (PCR).PCR reaction condition：95 DEG C of denaturations 5min, 95 DEG C of degeneration
30s, 58 DEG C of annealing 30s, 72 DEG C of extension 90s, 35 circulations, 72 DEG C of final extension 10min, 4 DEG C of preservations.PCR primer uses 0.8%
Agarose gel separate, purification with reclaim after be sequenced.Be sequenced row using NCBIBlast compare analysis, determine bacterial strain Z-1 from
Belong to Alcaligenes (Alcaligenes sp.).
The 16S rDNA sequence of Alcaligenes (Alcaligenes sp.) Z-1 refers to sequence table, and this bacterial strain is in 2016 6
The moon is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 2nd, and address is the Chaoyang District, Beijing City North Star
West Road 1 institute 3, preserves registration number CGMCC NO.12585.
3. the cultural method of the Alcaligenes Z-1 bacterial strain of the present invention, comprises the steps：
1) preparation of seed liquor
A. solid medium：Carnis Bovis seu Bubali cream：10g, peptone：10g, NaCl：5g, glucose：10g, distilled water：1000ml,
Agar powder：18g, pH：7.0-7.2；
B. fluid medium：Carnis Bovis seu Bubali cream：10g, peptone：10g, NaCl：5g, glucose：10g, distilled water：1000ml,
pH：7.0-7.2；
C. by after (121 DEG C, the 30min) sterilizings of above-mentioned culture medium high-temperature vapour, fall in super-clean bench on the flat board of sterilizing,
Stand-by after culture medium solidifying；
D. bacterium Z-1 is inoculated on above-mentioned flat board by the method for line, flat board is inverted in 30 DEG C of incubator cultures 24
～36h treats that bacterium colony grows；
E. the well-grown Z-1 bacterium colony made even on plate with inoculating loop scrapes, and is inoculated in fluid medium, triangle is bottled
Liquid measure is 60%, in 30 DEG C of temperature, 120rpm, incubation time 24～36h to exponential phase, obtains seed liquor.
2) ferment tank
A. fermentation broth formula is：Glucose 15g/L, KH2PO45g/L, (NH4)2HPO46g/L, citric acid
0.9g/L, yeast extract 19.5g/L, trace element solution 5mL, defoamer 0.1%, distilled water 1000mL, adjust pH to 7.0.Micro-
Secondary element solution composition is：FeSO4·7H2O 10g/L, ZnSO4·7H2O 5.25g/L, CuSO4·5H2O 3g/L, H3BO3
0.3g/L, CoCl2.6H2O 0.04g, CaCl22.0g/L, Na2MoO4·2H2O 0.15g/L, distilled water 1000mL, pH7.0.
B. the Z-1 seed liquor above-mentioned culture being obtained, the inoculum concentration according to fermentation liquid total amount 2%～5% moves to fermentation tank,
Carry out fermentation culture, condition is：30 DEG C of tank temperature, tank pressure 0.03MPa, ventilation 1:1V/V/min, mixing speed 150rpm, fermentation
Time 12～18h, makes bacteria concentration in fermentation liquid be 3 × 109CFU/mL；
3) preservation of microbial inoculum
The zymocyte liquid of above-mentioned acquisition is staticly settled, concentrates 3～5 times, make liquid microbial inoculum, protect as in 4 DEG C of environment
Deposit.
4) use of microbial inoculum
During efficient composite bacteria agent uses, add aeration activation 0.5-1h, microbial inoculum dosage phase in tap water
Between, dosage is：Microbial inoculum amount/the water yield=0.1 ‰～2 ‰, can once add or add several times.
4.Z-1 bacterium processes certain and produces polyacrylamide factory waste water
Certain produces polyacrylamide factory waste water, and waste water conductance is 45000～60000us/cm, inorganic salt based on NaCl,
COD is 12000～18000mg/L, and wherein polyacrylamide provides COD content to account for total amount 15%～18%.Wastewater dilution 2～3
Times, with high-efficiency strain Z-1 process.Water inlet conductance is 16000～25000us/cm, and COD is about 5500～6000mg/L, gathers
Acrylamide provides COD content to account for total amount 5%～6%, adds Z-1 bacterium dosage to be 1 ‰, aerobic through aerobic 24～36h, goes out
Water COD reaches below 1500mg/L, and polyacrylamide provides COD content to account for total COD0.3%～0.5% of water outlet.
Embodiment 2：Z-1 bacterium processes certain offshore platform well-drilling waste water
In certain offshore platform well-drilling waste water, COD is 5000～7000mg/L as different from Example 1, wherein polypropylene acyl
Amine provides COD to account for the 5% of total COD, and polysulfonate class macromolecular substances provide COD to account for total COD and measure as 6%-10%, waste water conductance
For 27000～35000us/cm.Process this waste water using Z-1 bacterium, strain dosage is 2 ‰, after wherein aerobic 48h is processed, go out
Water COD reaches below 1500mg/L；Add growth promoter while adding strain, growth promoter provides COD to be about
200mg/L, water outlet COD reach below 500mg/L, and polyacrylamide, the content of polysulfonate class are respectively less than 0.2%.
Example 3：Z-1 bacterium processes certain natural gas drilling waste water
Processing waste water as different from Example 1 is Shanxi somewhere natural gas drilling waste water, and water outlet COD is 5500mg/L,
Conductance is 25000～28000us/cm, and the wherein macromole such as polysulfonate class accounts for 8%, adds 0.5 ‰ Z-1 bacterium and processes, goes out after 48h
Water COD reaches 500mg/L, and the macromole ratio such as polysulfonate class is below 0.3%.
Inventive principle
Alcaligenes Z-1 is one kind energy degradation of polypropylene amide of separation screening from well-drilling waste water reservoir bed mud, polysulfonate class
Antibacterial Deng larger molecular organicses.The high-salt wastewater living environment that Z-1 is 15000～38000us/cm in conductance, produces first
There is to polymer the biological hydrolysis enzyme of certain degradation capability, the first side chain of attack polymer, it is carried out with de- chain and decomposes, profit
The carbon source being produced with catabolic process and nitrogen source, sulphur source maintain and provide needed for own growth.After side chain decomposes, main chain is exposed again
Outward, in the presence of Z-1 can secrete monooxygenase, so that main polymer chain is gradually disconnected, be fractured into the material of molecular weight, from
And so that microorganism is possibly realized by the use of polymerization macromole as carbon source.A series of oxidation reaction participating in through multiple microbial enzymes
The polyacrylamide of long-chain is just broken off into short chain, comparatively short strand further by microorganism using thus creating second
Acid, thus the macromolecule polyalcohol such as polyacrylamide, polysulfonate class is utilized by the microorganism, utilizes Organic substance to maintain certainly in microorganism
During body growth and breeding, reach the purpose that waste water COD is degraded.
SEQUENCE LISTING
<110>Company limited of CNOOC Tianjin Chemical Research ＆ Design Institute
CNOOC Energy Development Co., Ltd
<120>One plant of Alcaligenes and its application in high salt high-COD waste water
<130> THY-16-63
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1387
<212> DNA
<213> Alcaligenes sp.
<400> 1
gagagagctt gctctcttgg tggcgagtgg cggacgggtg agtaatatat cggaacgtgc 60
ccagtagcgg gggataacta ctcgaaagag tggctaatac cgcatacgcc ctacggggga 120
aaggggggga ttcttcggaa cctctcacta ttggagcggc cgatatcgga ttagctagtt 180
ggtggggtaa aggctcacca aggcaacgat ccgtagctgg tttgagagga cgaccagcca 240
cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga attttggaca 300
atgggggaaa ccctgatcca gccatcccgc gtgtatgatg aaggccttcg ggttgtaaag 360
tacttttggc agagaagaaa aggtatctcc taatacgaga tactgctgac ggtatctgca 420
gaataagcac cggctaacta cgtgccagca gccgcggtaa tacgtagggt gcaagcgtta 480
atcggaatta ctgggcgtaa agcgtgtgta ggcggttcgg aaagaaagat gtgaaatccc 540
agggctcaac cttggaactg catttttaac tgccgagcta gagtatgtca gaggggggta 600
gaattccacg tgtagcagtg aaatgcgtag atatgtggag gaataccgat ggcgaaggca 660
gccccctggg ataatactga cgctcagaca cgaaagcgtg gggagcaaac aggattagat 720
accctggtag tccacgccct aaacgatgtc aactagctgt tggggccgtt aggccttagt 780
agcgcagcta acgcgtgaag ttgaccgcct ggggagtacg gtcgcaagat taaaactcaa 840
aggaattgac ggggacccgc acaagcggtg gatgatgtgg attaattcga tgcaacgcga 900
aaaaccttac ctacccttga catgtctgga atcccgaaga gatttgggag tgctcgcaag 960
agaaccggaa cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta 1020
agtcccgcaa cgagcgcaac ccttgtcatt agttgctacg caagagcact ctaatgagac 1080
tgccggtgac aaaccggagg aaggtgggga tgacgtcaag tcctcatggc ccttatgggt 1140
agggcttcac acgtcataca atggtcggga cagagggtcg ccaacccgcg agggggagcc 1200
aatctcagaa acccgatcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg 1260
aatcgctagt aatcgcggat cagaatgtcg cggtgaatac gttcccgggt cttgtacaca 1320
ccgcccgtca caccatggga gtgggttcac cagaagtagg tagcctaacc gaaggaggcg 1380
ctaccac 1387
Claims (6)
1. a kind of Alcaligenes (Alcaligenes sp.) Z-1 is it is characterised in that the deposit number of described Alcaligenes Z-1 is
CGMCC No.12585.
2. the Alcaligenes described in a kind of claim 1 (Alcaligenes sp.) Z-1 answering in the high poly- wastewater treatment of high salt
With it is characterised in that in described high-salt wastewater dissolved oxygen amount be 2.0～7.0mg/L.
3. application according to claim 2 it is characterised in that the inorganic salt in the high poly- waste water of described high salt be NaCl,
CaCl2, MgCl2；Conductance is 15000～38000us/cm, and COD is 5000～7000mg/L, and polymer provides COD to account for total COD ratio
Example is 1%～20%.
4. application according to claim 2 is it is characterised in that add growth promoter in the high poly- waste water of described high salt,
Described production accelerator is selected from monosaccharide, small molecular organic acid, alcohols one or more of which.
5. application according to claim 2 is it is characterised in that the COD that the dosage of described growth promoter provides is
The 5 ‰ -5% of the high poly- waste water COD of former high salt.
6. application according to claim 2 is it is characterised in that the dosage of described Alcaligenes Z-1 is high poly- useless for high salt
The 0.1wt ‰ to 2wt ‰ of water.
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| CN201610979936.2A CN106399200B (en) | 2016-11-08 | 2016-11-08 | Alcaligenes and application thereof in high-salt high-polymer wastewater |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109650557A (en) * | 2019-02-14 | 2019-04-19 | 上海海事大学 | A kind of method, microorganism antisludging agent and purposes reducing Calcium in Seawater magnesium ion content |
| WO2022250694A1 (en) * | 2021-05-28 | 2022-12-01 | Kimberly-Clark Worldwide, Inc. | Optimization of a halophilic phb depolymerase for industrial applications |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109650557A (en) * | 2019-02-14 | 2019-04-19 | 上海海事大学 | A kind of method, microorganism antisludging agent and purposes reducing Calcium in Seawater magnesium ion content |
| CN109650557B (en) * | 2019-02-14 | 2021-10-08 | 上海海事大学 | Method for reducing content of calcium and magnesium ions in seawater, microbial scale inhibitor and application |
| WO2022250694A1 (en) * | 2021-05-28 | 2022-12-01 | Kimberly-Clark Worldwide, Inc. | Optimization of a halophilic phb depolymerase for industrial applications |
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| Publication number | Publication date |
|---|---|
| CN106399200B (en) | 2019-12-10 |
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