CN102643748A - Sludge-reduced microorganism combined dominant population in sewage processing - Google Patents

Sludge-reduced microorganism combined dominant population in sewage processing Download PDF

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Publication number
CN102643748A
CN102643748A CN2012101445484A CN201210144548A CN102643748A CN 102643748 A CN102643748 A CN 102643748A CN 2012101445484 A CN2012101445484 A CN 2012101445484A CN 201210144548 A CN201210144548 A CN 201210144548A CN 102643748 A CN102643748 A CN 102643748A
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China
Prior art keywords
nocardia
weight
gram
accounts
substratum
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CN2012101445484A
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Chinese (zh)
Inventor
刘建伦
张景致
杨艺
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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Treatment Of Sludge (AREA)

Abstract

The invention provides a sludge-reduced microorganism combined dominant population in sewage processing, which mainly comprises salmon-color nocardia, rhodopseudomonas palustris, scarlet nocardia, nocardia coralline, thermophilic myceliophthora, pseudomonas aeruginosa, sucroclastic pyrolysis cellulophaga and cryytococcus neoformans. The strains are respectively cultured in expending mode in a normal method and then performed with submerged fermentation by being mixed according to required proportion, the microorganism combined dominant population greatly reduces generation amount of sludge in urban sewage processing under the condition that original sewage processing devices, processes and methods are not changed and effectively solves the difficult-to-overcome problem of a large amount of surplus sludge in sewage processing for a long time.

Description

The mikrobe combination advantage flora of mud decrement in the WWT
Technical field
The invention provides a kind of a large amount of mikrobe combination advantage floras that reduce the generation of mud when being used for trade effluent and city domestic sewage and handling.
Background technology
Increase along with Chinese society expanding economy and urban population; Developing rapidly of sewage treatment industry; The quantity of sewage work also rises significantly, and the quantity of ending municipal wastewater treatment plant in 2010 has reached more than 2000, and the processing power of sewage is from the m seventies 600000 in 20th century 3/ d is increased to 7,500 ten thousand m 3/ d, wastewater treatment rate reaches 65%, produces a large amount of mud in the wastewater treatment process, and its water ratio is high; Organic content is high, and it is smelly to rot easily, comprehends the formation secondary pollution like improper conducting oneself well; Handle these mud, investment is big, and working cost is high; The land occupation area is big, and the time is long, and the pollution problem meeting is more serious.
(summary of the invention)
The present invention provides the microorganism combination advantage flora of mud decrement in a kind of sewage disposal; Microorganism consumes more organic matter by the internal respiration effect; And microorganism can generate a large amount of hydrolysis ectoenzymes; Under synergy; Utilize the complex relationship between microorganism, energy is degradable organic pollutant and suspension rapidly, changes the colourity of sewage; Eliminate stink, decomposing harmful substances.Begin from the source; Under the situation that does not change original sewage disposal device, operation scheme, technology, method; Through to trade effluent; A large amount of growing amounts that reduce mud during city domestic sewage is handled have solved effectively and in WWT, have been difficult to a large amount of excess sludge problems of overcoming for a long time.
Working method
Method by routine; Respectively bacterial classification is received enlarged culturing in the slant medium separately, 28 ℃~60 ℃ of culture temperature, incubation time is 3~10 days; Aerobic fermentation; Cultivate through shaking table, receive seeding tank in 5%~15% ratio and add the substratum submerged fermentation, become finished product by seeding tank switching large-tonnage ferment tank again.
Combination advantage flora of the present invention adds this bacterial classification and adds 0.001~0.5% by every cube of waste water in handling trade effluent and city domestic sewage.
(1), salmon look Nocardia bacteria (Nocardia salmonicolor) cultivates 121 ℃ of 20 minutes high-temperature sterilizations of substratum, leavening temperature is 28 ℃.
Substratum title (PH7.2) Weight (gram)
Yeast 4
Peptone 5
Glucose 4
Water 1000mL
(2), through 121 ℃ of 20 minutes high-temperature sterilizations, leavening temperature is 28 ℃~30 ℃ to Rhodopseudomonas palustris (Rhodopseudomonas palustris) with substratum.
Substratum title (PH6.8) Weight (gram)
Yeast 3
Peptone 3
CaCl 2 3
MgSO 4 2
Water 1000mL
(3), nocardia rudra (Nocardia rubropertincta) cultivates substratum through 121 ℃ of 20 minutes high-temperature sterilizations, leavening temperature is 28 ℃.
Substratum title (PH7.2) Weight (gram)
Glucose 6
K 2HPO 4 0.5
Water 1000mL
(4), coral Nocardia bacteria (Nocardia corallina) cultivates substratum through 121 ℃ of 20 minutes high-temperature sterilizations,, leavening temperature is 28 ℃.
Substratum title (PH7.0) Weight (gram)
Peptone 3
Beef leaching thing 3
NaCl 5
Water 1000mL
(5), thermophilic ruin the silk mould (a Myceliophthora thermophila) substratum is cultivated through 121 ℃ of 20 minutes high-temperature sterilizations,, leavening temperature is 45~60 ℃.
Substratum title (PH nature) Weight (gram)
Yam leaching thing 1L
Glucose 15
(6), Pseudomonas aeruginosa (Pseudomonas aeruginosa) cultivates substratum through 121 ℃ of 20 minutes high-temperature sterilizations, leavening temperature is 30 ℃.
Substratum title (PH7.0) Weight (gram)
Peptone 10
Beef leaching thing 3
NaCl 5
Water 1000mL
(7), separate sugared pyrolysis CELLULOLYTIC BACTERIUM (Caldicellulosiruptor) substratum cultivated through 121 ℃ of 20 minutes high-temperature sterilizations, leavening temperature is 40~60 ℃.
Substratum title (PH7.2) Weight (gram)
Yeast extract paste 1
NH 4Cl 2 1.5
NaCl 2
FeCl 3 4
Water 1000mL
(8), geotrichum candidum (Geotrichum candidum) cultivates substratum through 121 ℃ of 20 minutes high-temperature sterilizations, leavening temperature is 25 ℃~28 ℃.
Substratum title (PH nature) Weight (gram)
The 50Be wort 1
Agar 15
With the top fermentation proportional mixing, contain several 106~1,010 hundred million/mL of bacterium, reach more than 80% at processing trade effluent and city domestic sewage clearance.

Claims (2)

1. the mikrobe combination advantage flora of mud decrement in the WWT is characterized in that; Salmon look Nocardia bacteria (Nocardia salmonicolor), Rhodopseudomonas palustris (Rhodopseudomonas palustris), nocardia rudra (Nocardia rubropertincta), coral Nocardia bacteria (Nocardia corallina), thermophilicly ruin silk mould (Myceliophthora thermophila), a Pseudomonas aeruginosa (Pseudomonas aeruginosa), separate sugared pyrolysis CELLULOLYTIC BACTERIUM (Caldicellulosiruptor), geotrichum candidum (Geotrichum candidum), weight Nocardia salmonicolor accounts for 5~13% in proportion, Rhodopseudomonas palustris accounts for 7~14% for 8 kinds of bacterium, Clostridium beijerinckii accounts for 12~18%, Nocardia corallina accounts for 4~11%, Clostridium thermocellum accounts for 3~10%, Pseudomonas aeruginosa accounts for 14~19%, Caldicellulosiruptor accounts for 12~17%, Geotrichum candidum accounts for 3~6%.
2. the mikrobe combination advantage flora of mud decrement in WWT is characterized in that; Mud can reduce the generation of mud in a large number when handling trade effluent and city domestic sewage.This dominant microflora adds this bacterial classification and adds 0.001~0.5% by every cube of waste water in handling trade effluent and city domestic sewage.More than each bacterial classification to distinguish enlarged culturing, the proportioning of its substratum is following:
(1), salmon look Nocardia bacteria (Nocardia salmonicolor)
Substratum title (PH7.2) Weight (gram) Yeast 4 Peptone 5 Glucose 4 Water 1000mL
(2), Rhodopseudomonas palustris (Rhodopseudomonas palustris)
Substratum title (PH6.8) Weight (gram) Yeast 3 Peptone 3 CaCl 2 3 MgSO 4 2 Water 1000mL
(3), nocardia rudra (Nocardia rubropertincta)
Substratum title (PH7.2) Weight (gram) Glucose 6 K 2HPO 4 0.5
Water 1000mL
(4), coral Nocardia bacteria (Nocardia corallina)
Substratum title (PH7.0) Weight (gram) Peptone 3 Beef leaching thing 3 NaCl 5 Water 1000mL
(5), the thermophilic silk mould (Myceliophthora thermophila) of ruining
Substratum title (PH nature) Weight (gram) Yam leaching thing 1L Glucose 16
(6), Pseudomonas aeruginosa (Pseudomonas aeruginosa)
Substratum title (PH7.0) Weight (gram) Peptone 10 Beef leaching thing 3 NaCl 5 Water 1000mL
(7), separate sugared pyrolysis CELLULOLYTIC BACTERIUM (Caldicellulosiruptor)
Substratum title (PH7.2) Weight (gram) Yeast extract paste 1 NH 4Cl 2 1.5 NaCl 2 FeCl 3 4
Water 1000mL
(8), geotrichum candidum (Geotrichum candidum)
Substratum title (PH nature) Weight (gram) The 50Be wort 1 Agar 15
CN2012101445484A 2012-05-09 2012-05-09 Sludge-reduced microorganism combined dominant population in sewage processing Pending CN102643748A (en)

Priority Applications (1)

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Application Number Priority Date Filing Date Title
CN2012101445484A CN102643748A (en) 2012-05-09 2012-05-09 Sludge-reduced microorganism combined dominant population in sewage processing

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108238677A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body cadmium ion enrichment treatment device
CN108238679A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body lead ion enrichment treatment device
CN108238678A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body copper ion enrichment treatment device
CN110510753A (en) * 2019-08-20 2019-11-29 深圳市科世纪环保科技有限公司 A kind of technique that organic enzymes are used for mud decrement

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CN102250805A (en) * 2011-07-01 2011-11-23 浙江商达环保有限公司 High-density culture method of composite microbial agent for papermaking wastewater treatment
CN102381822A (en) * 2011-07-18 2012-03-21 刘建伦 Multi-strain compound microbial high-temperature rapid-digestion sludge harmless treatment method

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CN1269828A (en) * 1997-08-14 2000-10-11 诺沃挪第克公司 Reduction of malodour
CN1718548A (en) * 2005-05-16 2006-01-11 上海市政工程设计研究院 Treatment method of industrial waste water
CN101466843A (en) * 2006-05-01 2009-06-24 达特默斯大学托管会 Process for the hydrolysis of cellulose mediated by ternary complexes of cellulose, clostridium thermocellum cells, and cellulase expressed by these cells
CN101074142A (en) * 2007-06-20 2007-11-21 上海裕麒环境技术服务有限公司 Method for circulating and reutilizing sludge during process of sewage
CN101205101A (en) * 2007-12-13 2008-06-25 武汉工业学院 Treatment method of oil-containing waste water
CN102050523A (en) * 2010-12-29 2011-05-11 赵亚勋 Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism
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CN102381822A (en) * 2011-07-18 2012-03-21 刘建伦 Multi-strain compound microbial high-temperature rapid-digestion sludge harmless treatment method

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108238677A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body cadmium ion enrichment treatment device
CN108238679A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body lead ion enrichment treatment device
CN108238678A (en) * 2016-12-27 2018-07-03 中国科学院宁波城市环境观测研究站 A kind of water body copper ion enrichment treatment device
CN110510753A (en) * 2019-08-20 2019-11-29 深圳市科世纪环保科技有限公司 A kind of technique that organic enzymes are used for mud decrement

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