CN101205101A - Treatment method of oil-containing waste water - Google Patents

Treatment method of oil-containing waste water Download PDF

Info

Publication number
CN101205101A
CN101205101A CNA2007101688771A CN200710168877A CN101205101A CN 101205101 A CN101205101 A CN 101205101A CN A2007101688771 A CNA2007101688771 A CN A2007101688771A CN 200710168877 A CN200710168877 A CN 200710168877A CN 101205101 A CN101205101 A CN 101205101A
Authority
CN
China
Prior art keywords
microorganism
rhodococcus
pseudomonas
lactobacilli
hydrophobicity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CNA2007101688771A
Other languages
Chinese (zh)
Other versions
CN101205101B (en
Inventor
刘晔
刘大川
尹洪波
路炬晶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wuhan Polytechnic University
Original Assignee
Wuhan Polytechnic University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuhan Polytechnic University filed Critical Wuhan Polytechnic University
Priority to CN2007101688771A priority Critical patent/CN101205101B/en
Publication of CN101205101A publication Critical patent/CN101205101A/en
Application granted granted Critical
Publication of CN101205101B publication Critical patent/CN101205101B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a method for treating oily wastewater by making hydrophobic microorganism cell as absorbent, which consists of an absorbing step-the hydrophobic microorganism cell is put into and mixed with the oily wastewater to absorb oil body in the oily wastewater; and a separating step- cell-oil body complexes formed by the absorbing step is separated from the oily wastewater.The cell-oil body complexes which are easy to be separated can be formed after absorbing; after the cell-oil body complexes are separated from the wastewater, COD and BOD of the wastewater can be lowered, and oil body can be extracted from the cell-oil body complexes to be reused or the complexes can be used as ferment medium composition of other microorganism or as fuel.The method can be widely used with an overall low cost.

Description

Oily wastewater treatment method
Technical field
The present invention relates to absorption method and handle oil-containing industrial water and its recovery method as resource, perhaps relate to the microorganism treatment of waste water.
Background technology
Oil-containing industrial water is the trade effluent of class wide material sources, in the extraction of oil production, transportation and the course of processing, vegetable and animals oils and the course of processing and mechanical workout and cleaning process a large amount of dischargings is arranged all.At present, mainly adopt means such as oil removal, air supporting, coagulating sedimentation and biological degradation at oil-containing industrial water, remove oil body in the waste water with the COD and the BOD value that reduce waste water and make it to reach emission standard.Yet, adopt these processing modes not only will consume a large amount of energy and chemicals, and can not produce any economic benefit.If the oil body of various states in the oily(waste)water can be reclaimed and take suitable mode to utilize again, thereby realize the resource utilization of oily(waste)water, will effectively reduce the total cost that oily(waste)water is handled.
Some investigators propose to utilize the adsorbents adsorb waste water oil body of chemosynthesis and reclaimed, and reduce the method for wastewater through organic matter content simultaneously.Introduced a kind of preparation and using method of high hydrophobicity resin as Chinese patent 99116032.0, processing and oil body that the sorbent material that this high hydrophobicity resin can be used as oil body is used for oily(waste)water reclaim.Yet the chemosynthesis sorbent material exists manufacturing cost higher relatively, needs problems such as recovery.
There is not at present biosorption process to handle the report of oily(waste)water as yet.
Summary of the invention
The objective of the invention is weak point, a kind of method of handling oily(waste)water and separating oil body with the microorganism absorption method is provided at present oily(waste)water treatment technology.
Its ultimate principle is, utilizes the chemotaxis of certain micro-organisms, makes microorganism cells spontaneously be assigned to the higher zone of oil body content in waste water; Utilize the adsorptive power of microorganism cells to hydrophobic substrate (hydro carbons, lipid), the oil body in the absorption oily(waste)water forms cell-oil body mixture.Again formed cell-oil body mixture is separated with waste water, thereby reclaim oil body, and reduce organic content in the waste water.
The invention provides the method for a kind of hydrophobicity microorganism cells, comprise adsorption step: the hydrophobicity microorganism cells is dropped into oily(waste)water and mixing, with the oil body in the absorption oily(waste)water as the sorbent treatment oily(waste)water; With separating step: the cell-oil body mixture that forms in the described adsorption step is separated from oily(waste)water.
Preferably, the hydrophobicity microorganism of using in the specific embodiment of the invention is selected from: aurantia small bacillus (Exiguobacterium aurantiacum), onion bulkholderia cepasea (Burkholderiacepacia), pseudomonas stanieri (Pseudomonas stutzeri), Nocardia bacteria (Nocardiodessp.), rhodococcus (Rhodococcus sp.), mycobacterium (Mycobacterium), sphingomonas paucimobilis (Sphingomonas paucimobilis var.), utmost point candiyeast (Candidaantarctica), pseudomonas testosteroni (Comamonas testosteroni), actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans), actinomyces naeslundii (Actinomycesnaeslundii), Candida albicans (Candida albicans), enterococcus faecalis (Enterococcifaecalis), Lactobacterium acidophilum (Lactobacilli acidophilus), lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus), lactobacillus fermentum (Lactobacilli fermentum), Bacterium lacticum (Lactobacilli gasseri), Lactobacillus Jensenii (Lactobacilli jensenii), plant lactobacillus (Lactobacilli plantarum), Pseudomonas aeruginosa (Pseudomonas aeruginosa), staphylococcus epidermidis (Staphylococci epidermidis), Gall's chain coccus (Streptococci gordonii), Streptococcus mitis (Streptococci mitis), Streptococcus oralis (Streptococci oralis), Streptococcus sanguis (Streptococci sanguis), subtilis (Bacillus subtilis), maltose candiyeast (Candida maltosa), inferior sieve is separated fat yeast (Yarrowia lipolytica), Rhodococcus (Rhodococcus erythropolis), many wrinkle general Salmonellas (Prauserella rugosa), red-purple rhodococcus (Rhodococcus rhodochrous), sweet pea band rhodococcus (Rhodococcus fascians), Pseudomonas fluorescens (Pseudomonas fluorescens), acinetobacter calcoaceticus (Acineobacter sp.), Acinetobacter calcoaceticus (Acineobacter calcoaceticus), pseudomonas putida (Pseudomonas putida), accidental mycobacterium (Mycobacterium fortuitum), pleat streptomycete (Streptyomycesplicatus), pseudomonas mendocina (Pseudomonas mendocina), or their arbitrary combination.
Preferably, the hydrophobicity microorganism of using in embodiments of the present invention is selected from: aurantia small bacillus (Exiguobacterium aurantiacum) NCDO 2321 (T), onion bulkholderia cepasea (Burkholderia cepacia) LMG 12614t2, pseudomonas stanieri (Pseudomonasstutzeri) AN10, Nocardia bacteria (Nocardiodes sp.) KP7, rhodococcus (Rhodococcussp.) I24, mycobacterium (Mycobacterium) PYR-1, sphingomonas paucimobilis (Sphingomonas paucimobilis var.) EPA505, antarctic candida (Candidaantarctica) T-34, pseudomonas testosteroni (Comamonas testosteroni) B-356, actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1098, actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1099, actinomyces naeslundii (Actinomyces naeslundii) PK29, Candida albicans (Candida albicans) ATCC 10261, enterococcus faecalis (Enterococci faecalis) C1030, Lactobacterium acidophilum (Lactobacilli acidophilus) T13, Lactobacterium acidophilum (Lactobacilli acidophilus) RC14, Lactobacterium acidophilum (Lactobacilli acidophilus) 68, Lactobacterium acidophilum (Lactobacilliacidophilus) 75, lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus) 81, lactobacillus fermentum (Lactobacilli fermentum) B54, Bacterium lacticum (Lactobacilli gasseri) 56, Bacterium lacticum (Lactobacilli gasseri) 60, Lactobacillus Jensenii (Lactobacilli jensenii) RC28, plant lactobacillus (Lactobacilli plantarum) RC20, Pseudomonas aeruginosa (Pseudomonasaeruginosa) AK1, staphylococcus epidermidis (Staphylococci epidermidis) 3294, staphylococcus epidermidis (Staphylococci epidermidis) 3081, staphylococcus epidermidis (Staphylococciepidermidis) 26741, staphylococcus epidermidis (Staphylococci epidermidis) 26512, Gall's chain coccus (Streptococci gordonii) NCTC7869, Streptococcus mitis (Streptococcimitis) BA, Streptococcus mitis (Streptococci mitis) BMS, Streptococcus mitis (Streptococcimitis) T9, Streptococcus oralis (Streptococci oralis) PK1317, Streptococcus oralis (Streptococcioralis) H1, Streptococcus sanguis (Streptococci sanguis) CR311, subtilis (Bacillussubtilis) ATCC7058, subtilis (Bacillus subtilis) ATCC15811, maltose candiyeast (Candida maltosa) EH60, inferior sieve is separated fat yeast (Yarrowia lipolytica) EH59, Rhodococcus (Rhodococcus erythropolis) NRRL B-16531, many wrinkle general Salmonella (Prauserella rugosa) NRRL B-2295, red-purple rhodococcus (Rhodococcus rhodochrous) NCIMB 12566, Rhodococcus (Rhodococcus erythropolis) ATCC 4277T, sweet pea band rhodococcus (Rhodococcus fascians) DSM 20669, Pseudomonas fluorescens (Pseudomonasfluorescens) CHA0, acinetobacter calcoaceticus (Acineobacter sp.) ADP-1, Acinetobacter calcoaceticus (Acineobacter calcoaceticus) ATCC 33304, rhodococcus (Rhodococcussp.) Q15, pseudomonas putida (Pseudomonas putida) ATCC 29347, accidental mycobacterium (Mycobacterium fortuitum) CGMCC 1.514, accidental mycobacterium (Mycobacteriumfortuitum) CGMCC 1.513, pleat streptomycete (Streptyomyces plicatus) KCC30, pleat streptomycete (Streptyomyces plicatu) sKCC42, pseudomonas mendocina (Pseudomonasmendocina) CGMCC 1.1768, Aspergillus nidulans (Aspergillus nidulans) CCIC2198 and their mutant strain or their combination arbitrarily.
In the specific embodiment of the invention, use hydrophobic value more than or equal to 30% microorganism cells as described hydrophobicity microorganism cells.
In the specific embodiment of the invention, described oil is mineral oil, vegetable and animals oils or synthetic oil.
In the specific embodiment of the invention, described adsorption step comprises at least one in following two processes: the microorganism amplification procedure, described hydrophobicity microorganism is dropped into oily(waste)water, mixing, blowing air under the temperature that is fit to this hydrophobicity microorganism growth make this hydrophobicity microorganism cells obtain amplification; With the microorganism adsorption process, be fit under the temperature of described hydrophobicity microorganism cells oil suction effect, the mixture of described hydrophobicity microorganism and oily(waste)water is stirred.
In a preferred embodiment of this invention, before described adsorption step, also comprise modified step, described modified step is: waste water is warming up to more than 40 ℃, perhaps adds fungistat in waste water, to suppress original microbial growth in the waste water.
In a preferred embodiment of this invention, described modified step is: waste water is warming up to 40-70 ℃, kept 0.5-10 hour; The fungistat that perhaps adds 0.01%-10% in waste water is handled 12-48h, and described fungistat comprises hydrogen peroxide, Peracetic Acid, acidic oxidized electric potential water, alkaline oxygenated current potential water or its combination; Perhaps be described hyperthermic treatment and described fungistat treatment combination.
In another embodiment of the present invention, described hydrophobicity microorganism cells is a dead cell.
Advantage of the present invention is, process hydrophobicity microorganism absorption oil body can form and be easy to isolated cells-oil body mixture, behind isolated cell from waste water-oil body mixture, not only can reduce the COD and the BOD of waste water, and can from mixture, the extract oil body weight newly utilize, perhaps this mixture is acted as a fuel or the medium component of other microbiological processes uses.Because the inventive method adopts microorganism cells as sorbent material, the sorbent treatment of chemosynthesis is compared, the advantage of this processing mode is: (1) sorbent material is the microorganism cells body, be that microorganism utilizes the spontaneous growth of nutritive substance in the waste water to produce, do not have manufacturing cost basically and need not to reclaim; (2) make full use of the motion of microorganism itself and adjust ability, compare with chemosorbent and have better choice, and can reduce mechanical energy consumption; (3) microorganism cells can increase at short notice in a large number, can further improve adsorption area, thereby improves adsorption effect.Also have, because the inventive method mainly utilizes the hydrophobic adsorption of cell to oil body, compare with the method for microbiological deterioration oil removing, the metabolic enzyme system of oil body there are not special requirement, so the oily(waste)water of the applicable different oil type of the inventive method, thereby has the wider scope of application.
Embodiment
Main points of the present invention are, break through in the prior art with the conventional techniques thinking of oil body in microbiological deterioration or " eating up " waste water with the purification oily(waste)water, the hydrophobicity of utilizing the certain micro-organisms cell to have is directly adsorbed the waste water oil body, thereby provides a kind of with microorganism cells with water repellent surface method as the sorbent treatment oily(waste)water.Being used for the kind with microorganism of water repellent surface of the present invention has no particular limits, various microorganism cellss such as hydrophobic bacterium, actinomycetes, yeast and mould known or that occurring in nature exists all can use, those skilled in the art is easy to judge and obtain such microorganism, and has the dead cell that the microorganism of water repellent surface obtains can be used for the present invention too after deactivation.
In order to make those skilled in the art can implement the present invention more easily, below the present invention's used " microorganism cells with water repellent surface " is done more clearly explanation." microorganism cells " that this paper mentions and the identical implication of " hydrophobicity microorganism " expression with water repellent surface.
Belong to following (a) or (b) in the part institute description scope or cited microorganism all can be used for implementing the present invention.
(a) the existing known oil that can utilize is as degrading outside carbon source for growth or can be in born of the same parents/born of the same parents or transforming the microorganism of oil.
This quasi-microorganism is to utilization, degraded or the conversion of oil, generally all in the profit mixed system, carry out, it at first needs to utilize hydrophobic adsorptivity of himself or secreted tensio-active agent, with hydrophobic oil picked-up or be adsorbed in the cell or on cell walls/film or cell peripheral, that is to say, this quasi-microorganism has stronger adsorptivity to oils, can be used as the microorganism cells with water repellent surface and is used for the present invention.Industrial application and the existing a large amount of reports of research to this quasi-microorganism, those skilled in the art fully can be according to the retrieval of existing documents and materials, know the kind that can be used for microorganism of the present invention, also obtain many kinds from microbial preservation unit easily and can be used for implementing hydrophobicity microorganism of the present invention.
The microorganism that can be used as hydrophobicity microorganism use of the present invention includes, but not limited to the microorganism of following kind:
Aurantia small bacillus (Exiguobacterium aurantiacum)
Onion bulkholderia cepasea (Burkholderia cepacia)
Pseudomonas stanieri (Pseudomonas stutzeri)
Nocardia bacteria (Nocardiodes sp.)
Rhodococcus (Rhodococcus sp.)
Mycobacterium (Mycobacterium)
Sphingomonas paucimobilis (Sphingomonas paucimobilis var.)
Antarctic candida (Candida antarctica)
Pseudomonas testosteroni (Comamonas testosteroni)
Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans)
Actinomyces naeslundii (Actinomyces naeslundii)
Candida albicans (Candida albicans)
Enterococcus faecalis (Enterococci faecalis)
Lactobacterium acidophilum (Lactobacilli acidophilus)
Lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus)
Lactobacillus fermentum (Lactobacilli fermentum)
Bacterium lacticum (Lactobacilli gasseri)
Lactobacillus Jensenii (Lactobacilli jensenii)
Plant lactobacillus (Lactobacilli plantarum)
Pseudomonas aeruginosa (Pseudomonas aeruginosa)
Staphylococcus epidermidis (Staphylococci epidermidis)
Gall's chain coccus (Streptococci gordonii)
Streptococcus mitis (Streptococci mitis)
Streptococcus oralis (Streptococci oralis)
Streptococcus sanguis (Streptococci sanguis)
Subtilis (Bacillus subtilis)
Maltose candiyeast (Candida maltosa)
Inferior sieve is separated fat yeast (Yarrowial lipolytica)
Rhodococcus (Rhodococcus erythropolis)
Many wrinkle general Salmonellas (Prauserella rugosa)
Red-purple rhodococcus (Rhodococcus rhodochrous)
Sweet pea band rhodococcus (Rhodococcus fascians)
Pseudomonas fluorescens (Pseudomonas fluorescens)
Acinetobacter calcoaceticus (Acineobacter sp.)
Acinetobacter calcoaceticus (Acineobacter calcoaceticus)
Pseudomonas putida (Pseudomonas putida)
Accidental mycobacterium (Mycobacterium fortuitum)
Pleat streptomycete (Streptyomyces plicatus)
Pseudomonas mendocina (Pseudomonas mendocina)
Aspergillus nidulans (Aspergillus nidulans)
More specifically, as the mentioned microorganism examples of types, table 1 has been listed the example and the pertinent literature of partially hydrophobic microorganism strains:
Table 1
Numbering The bacterial strain name Hydrophobic value %
1 Aurantia small bacillus (Exiguobacterium aurantiacum) NCDO 2321 (T) 53
2 Onion bulkholderia cepasea (Burkholderia cepacia) LMG 12614t2 62
Above-mentioned 1,2 bacterial strain pertinent literatures: Mohanty G, Mukherji S.Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia.International Biodeterionration and Biodegradation, in press, 2007
3 Pseudomonas stanieri (Pseudomonas stutzeri) AN10 59
4 Nocardia bacteria (Nocardiodes sp.) KP7 66
Document: Saito A, Iwabuchi T, Harayama S.A novel phenanthrene dioxygenase from Nocardioides sp.Strain KP7:expression in Escherichia coli.J.Bacteriol., 2000,182:2134-2141
5 Rhodococcus (Rhodococcus sp.) I24 69
Document: Treadway S L, Yanagimachi K S, Lankenau E, et al.Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24.Appl.Microbiol.Biotechnol.1999,51:786-793
6 Mycobacterium (Mycobacterium) PYR-1 73
Document: Khan AA, Wang R F, Cao W W, et al.Molecular cloning, nucleotide sequence, and expression of genes encoding a ploycyclic aromatic ring dioxygenase from Mycobacterium sp.strain PYR-1.Appl.Environ. Microbiol.2001,67:3577-3585
7 Sphingomonas paucimobilis (Sphingomonaspaucimobilis var.) EPA505 68
Document: Story S P, Parker S H, Kline J D, et al.Identification of four structural genes and two putative promoters necessary for utilization of naphthalene, phenanthrene, and fluoranthene by Sphingomonas paucimobilis var.EPA505.Gene, 2000,260:155-169
8 Antarctic candida (Candida antarctica) T-34 54
Document: Hua Z, Chen J, Lun S, et al.Influence of biosufactants produced by Candida antarctica on surface properties of microorganism and biodegradation ofn-alkanes.Water Research, 2003,37:4143-4150
9 Pseudomonas testosteroni (Comamonas testosteroni) B-356 55
Document: Mehmannavaz R, Prasher S O, Ahmad D.Cell surface properties of rhizobial strains isolated from soils contaminated with hydrocarbons:hydrophobicity and adhesion to sandy soil.Process biochemistry, 2001,36:683-688
10 Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1098 66
11 Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1099 59
12 Actinomyces naeslundii (Actinomyces naeslundii) PK29 61
13 Candida albicans (Candida albicans) ATCC10261 58
14 Enterococcus faecalis (Enterococci faecalis) C1030 51
15 Lactobacterium acidophilum (Lactobacilli acidophilus) T13 72
16 Lactobacterium acidophilum (Lactobacilli acidophilus) RC14 68
17 Lactobacterium acidophilum (Lactobacilli acidophilus) 68 64
18 Lactobacterium acidophilum (Lactobacilli acidophilus) 75 59
19 Lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus) 81 58
20 Lactobacillus fermentum (Lactobacilli fermentum) B54 54
21 Bacterium lacticum (Lactobacilli gasseri) 56 68
22 Bacterium lacticum (Lactobacilli gasseri) 60 62
23 Lactobacillus Jensenii (Lactobacilli jensenii) RC28 71
24 Plant lactobacillus (Lactobacilli plantarum) RC20 64
25 Pseudomonas aeruginosa (Pseudomonas aeruginosa) AK1 58
26 Staphylococcus epidermidis (Staphylococci epidermidis) 3294 66
27 Staphylococcus epidermidis (Staphylococci epidermidis) 3081 63
28 Staphylococcus epidermidis (Staphylococci epidermidis) 26741 58
29 Staphylococcus epidermidis (Staphylococci epidermidis) 26512 59
30 Gall's chain coccus (Streptococci gordonii) NCTC7869 54
31 Streptococcus mitis (Streptococci mitis) BA 61
32 Streptococcus mitis (Streptococci mitis) BMS 56
33 Streptococcus mitis (Streptococci mitis) T9 57
34 Streptococcus oralis (Streptococci oralis) PK1317 64
35 Streptococcus oralis (Streptococci oralis) H1 67
36 Streptococcus sanguis (Streptococci sanguis) CR311 71
Document: van der Mei H C, Bos R, Busscher H J.A reference guide to microbial cell surface hydrophobicity based on contact angles.Colloids and Surfaces B:Biointerfaces 11,1998,11:213-221
37 Subtilis (Bacillus subtilis) ATCC7058 65
38 Subtilis (Bacillus subtils) ATCC15811 53
39 Maltose candiyeast (Candida maltosa) EH60 58
40 Inferior sieve is separated fat yeast (Yarrowia lipolytica) EH59 64
Document: Kaczorek E, Chrzanowski L, Pijanowska A, Olszanowski A.Yeast and bacteria cell hydrophobicity and hydrocarbon biodegradation in the presence of natural surfactants:rhamnolipides and saponins.2007, in press
41 Rhodococcus (Rhodococcus erythropolis) NRRL B-16531 68
42 Many wrinkle general Salmonella (Prauserella rugosa) NRRL B-2295 53
43 Red-purple rhodococcus (Rhodococcus rhodochrous) NCIMB 12566 77
44 Rhodococcus (Rhodococcus erythropolis) ATCC 4277T 59
45 Sweet pea band rhodococcus (Rhodococcus fascians) DSM 20669 70
Document: van Beilen J B, Smits T H M, Whyte L G et al.Alkane hydroxylase homologues in gram-positive strains.Environmental Microbiology, 2002,11:676-682
46 Pseudomonas fluorescens (Pseudomonas fluorescens) CHA0 52
47 Acinetobacter calcoaceticus (Acineobacter sp.) ADP-1 68
48 Acinetobacter calcoaceticus (Acineobacter calcoaceticus) ATCC 33304 73
49 Rhodococcus (Rhodococcus sp.) Q15 71
50 Pseudomonas putida (Pseudomonas putida) ATCC 29347 67
Document: Whyte L G, Schultz A, van Beilen J B, et al.Prevalence of alkane monooxygenase genes in arctic and antarctic hydrocarbon-contaminated and pristine soils.FEMS Microbiology Ecology, 2002,41:141-150
51 Accidental mycobacterium (Mycobacterium fortuitum) CGMCC 1.514 76
52 Accidental mycobacterium (Mycobacterium fortuitum) CGMCC 1.513 70
54 Pleat streptomycete (Streptyomyces plicatus) KCC30 62
55 Pleat streptomycete (Streptyomyces plicatu) sKCC42 59
56 Pseudomonas mendocina (Pseudomonas mendocina) CGMCC 1.1768 63
57 Aspergillus nidulans (Aspergillus nidulans) CCIC2198 52
The listed microorganism of table 1 comprises bacterium, actinomycetes, yeast and mould, they are used to handle oily(waste)water as the hydrophobicity microorganism in a preferred embodiment of the invention, these bacterial strains both can use separately, also can two kinds be used in combination, in addition can bacterium, actinomycetes, yeast and mould mix and use.
The mutant strain that should be understood that cited microorganism strains still belongs to and can implement hydrophobicity microorganism of the present invention as long as it has the hydrophobicity cell surface.
(b) hydrophobic value is greater than 30% microorganism cells.
" hydrophobic value " is to judge for convenience and obtain to be used for hydrophobicity microorganism of the present invention and the quantitative concept introduced, the hydrophobicity on its expression microorganism cells surface or to the adsorptive power of oil.
The measuring method of hydrophobic value: get 2mL optical density(OD) OD 600Value is microorganism cells suspension liquid of aqueous phase (call in the following text and treat side microorganism cells liquid, the optical density(OD) that records is designated as initial OD) between 0.1～0.2, injects 10 * 150mm Glass tubing, injects 0.1mL n-Hexadecane hydrocarbon in Glass tubing, places 30 ℃ of following incubations 10 minutes.Left standstill 30 seconds after 1 minute in vibration on the vortex oscillation device, after vibrating again 1 minute sample placed and left standstill under 30 ℃ 1 hour.Draw lower floor's enchylema with syringe, inject cuvette and measure OD 600Value (optical density(OD) that records is designated as absorption back OD).The value that the difference of initial OD and absorption back OD obtains divided by initial OD is the hydrophobic value of tested microorganism cells in percentage ratio.Measurement result is measured mean value with 3 times and is as the criterion.Above-mentioned OD value is blank determination with water with spectrophotometer.
Microorganism cells liquid to be measured:The microorganism cells that is used to measure hydrophobic value can be the diluent of bacterium, yeast, mould or actinomycetic nutrient solution or culture.Can use the suitable substratum and the culture condition of growth and breeding separately when cultivating these microorganisms.In culturing process, can on the basis of collective media, add or not add oil as substrate/inductor.The cell of the microorganism cells liquid that is used for measuring can be survived, and also can be the dead cell through inactivation treatment.Also have, treat that the cell in the side microorganism cells liquid can be the combination of different types of microorganisms cell.
Measure with the hydrophobic value measuring method and above-describedly to treat side microorganism cells liquid, if the gained hydrophobic value more than or equal to 30%, then this microorganism cells belongs to hydrophobicity microorganism cells of the present invention.Preferably, the hydrophobic value of hydrophobicity microorganism cells of the present invention is more than or equal to 50%, and more preferably greater than equaling 70%.
For more convenient, it is a kind of for obtaining the microbial culture method (type culture condition) of microorganism cells liquid to be measured that this paper provides: in the basic salt culture medium culturing micro-organisms of following component, and K 2HPO 4, 10g/L; NaH 2PO 4, 5g/L; (NH 4) 2SO 4, 2g/L; MgSO 4, 0.2g/L; CaCl 2, 2-3mg/L; FeSO 4, 1-2mg/L; Yeast powder, 100mg/L; Carbon source adopts n-Hexadecane 1.0g/L or Oleum Gossypii semen 1.0g/L, and culture temperature is 30 ℃.The hydrophobic value of listed each microorganism strains of table 1 is the hydrophobic value that this microorganism records after present method is cultivated.
Aforementioned (a) made specific descriptions from different angles to hydrophobicity microorganism of the present invention with (b) part.The microorganism that these two portions relate to can be an eclipsed.And in (a) the listed part known microorganisms, other known microorganisms and from the isolating microorganism of nature as long as its hydrophobic value, all belongs to the hydrophobicity microorganism that (b) part is defined more than or equal to 30%, all can be used for implementing the present invention.
Above-mentioned hydrophobic value measuring method promptly can be used to judge known or whether can be used as hydrophobicity microorganism of the present invention from the isolating microorganism of nature, also can be used as from the means of occurring in nature screening hydrophobicity microorganism.
There is no particular limitation to handled oily(waste)water for the inventive method, is generally at the extraction of oil production, transportation and the course of processing, vegetable and animals oils and the oily(waste)water in the course of processing and mechanical workout and the cleaning process.Hydrophobicity microorganism cells of the present invention only need utilize its surface hydrophobicity to adsorb hydrophobic oil, and need not to utilize specific enzyme to degrade or utilize specific oil, when therefore it is applied to oily(waste)water oily kind is not had special requirement, mineral oil, vegetable and animals oils or the synthetic oil (as industrial lubricant) etc. that are present in the waste water with liquid state suspension, emulsification or solubilising state are suitable for method of the present invention.
Before dropping into the adsorption step of hydrophobicity microorganism cells, can carry out modified step with the absorption oil body to oily(waste)water.Usually contain a large amount of microorganisms in the oil-containing industrial water, its population composition also has nothing in common with each other.Can increase for the hydrophobicity microorganism that guarantee to add becomes dominance population in the waste water, before dropping into the hydrophobicity microorganism, can implement modifier treatment to waste water as required, breed in a large number to suppress original microorganism, thereby make the absorption microorganism after access, obtain competitive edge and become the dominance population.Modifier treatment can be adopted physical method or chemical process.Physical method comprises that high temperature is antibacterial, particularly, wastewater temperature is adjusted to 40-70 ℃, keeps 0.5-10h; Chemical process comprises the fungistat processing, and particularly, the fungistat that adds 0.01%-10% in waste water is handled 12-48h, and fungistat can be hydrogen peroxide, Peracetic Acid and current potential water etc., and fungistat can be used alone or in combination.The use also capable of being combined of above-mentioned physical method and chemical process.
In adsorption step,, can be suitable for that this microorganism is increased rapidly, thereby the surface-area of sorbent material is significantly enlarged if the hydrophobicity microorganism of dropping into is a survivaling cell.The condition that is suitable for this microorganism growth is often referred in waste water blowing air, stir, keep the means of the temperature etc. of suitable breeding promotion microbial reproduction commonly used.And can regulate waste water ph according to microorganism used therefor kind and waste water composition, or add the nutritive substance of an amount of carbon, nitrogenous source and so on as required, the pH value is regulated and nutrition adds also and can add before or after dropping into the hydrophobicity microorganism.Those skilled in the art is easy to obtain by limited test the condition of described suitable used hydrophobicity microorganism growth amplification.Usually, preferable amplification condition is: waste water ph 5.0-8.0, temperature 25-40 ℃.
The benefit of a large amount of amplifications of hydrophobicity microorganism is, can remove the process of the preceding large scale culturing hydrophobicity microorganism cells of wastewater treatment from, and can make full use of original organic substance in the waste water, can save cost and can also reduce waste water COD, BOD extraly, can make waste water reach emission standard or significantly reduce subsequent disposal load.
Through above-mentioned stage based on the microorganism amplification, when hydrophobicity microorganism cells quantity is enough in the waste water, entering the microorganism adsorption process is the main stage, usually, adopt lower temperature and more weak stirring intensity will help the formation of cell-oil body mixture, and make it to assemble and become volume and the bigger solid particulate of intensity, thereby can reclaim by filtering separation easily.Usually, the preferable condition of adsorption process is: waste water ph 5.0-8.0, temperature 15-30 ℃.
Obviously, microorganism amplification procedure in the adsorption step and microorganism adsorption process are not well-separated from the time, mainly are by controlling different conditions, making one of them be in leading relatively status.In the actually operating, operational condition that is beneficial to amplification or the condition that is beneficial to absorption can be only kept, also the object of the invention can be reached.This is understanding easily for a person skilled in the art and implements.
In addition, tend to produce a large amount of useless cells after leavened prod is being extracted in microbial fermentation industry, these cells have hydrophobicity sometimes, and this moment, available these cells were handled oily(waste)water as the hydrophobicity microorganism cells.These cells can drop into oily(waste)water with the dead cell form and directly carry out the oil body adsorption process after deactivation, owing to there is not the microorganism amplification procedure, need the cell concentration of input bigger.The benefit of this method is not only can handle oily(waste)water, and the useless cell of other fermentation industry is utilized once more.
After finishing adsorption step, must be from waste water Separation and Recovery cell-oil body mixture.Cell-oil body mixture is the solid or the semisolid of particulate state, form such as cotton-shaped usually.Can be with conventional solid-liquid separating method isolated complex, as filtration, sedimentation etc.
Therefore the composition of oil body is simple relatively in the oily(waste)water that the particular industry class is discharged, and the oil body that reclaims through biology absorption has very high recycling and is worth, and the shape of cell-oil body mixture is become this recycling and created superior condition.
Adopt the adsorption treatment oily(waste)water, not only available energy reduces the COD and the BOD value of waste water, and oil body can be recycled, thereby can really realize waste water reclaiming.From this angle, Comparatively speaking adsorption treatment oily(waste)water and traditional biological degradation processing mode more can embody the characteristics of recycling economy, have bigger development potentiality.And the microorganism that present method proposed absorption is a kind of new oily(waste)water adsorption treatment technology, does not appear in the newspapers at present.Compare with the chemisorption processing of having reported, the advantage of this processing mode is: (1) sorbent material is the microorganism cells body, is that microorganism utilizes the spontaneous growth of nutritive substance in the waste water to produce, and does not have manufacturing cost basically and need not to reclaim; (2) make full use of the motion of microorganism itself and adjust ability, compare with chemosorbent and have better choice, and can reduce mechanical energy consumption; (3) microorganism cells can increase at short notice in a large number, can further improve adsorption area, thereby improves adsorption effect.
Below in conjunction with embodiment content of the present invention is described further.
[embodiment]
Used hydrophobicity microorganism cells sees before and states table 1.
Handled oily(waste)water kind sees Table 2.
The processing condition of modified step see Table 3.
The oily(waste)water treating processes:
A. seed culture, each hydrophobicity microorganism or its combination are inserted seed culture medium, cultivate by the condition that the conventional cultural method of each microorganism strains or each microbial preservation unit and above-mentioned pertinent literature provide, most microorganisms can adopt the Luria-Bertzani substratum to cultivate down at 28 ℃, and the part acidophilic microorganism can adopt the Luria-Bertzani substratum of pH5 to cultivate down at 28 ℃;
B. modified, for oily(waste)water, undertaken by hardening and tempering process shown in the table 3;
C. inoculation is got among a. nutrient solution and is inserted modified back waste water, makes that the hydrophobicity microorganism concn reaches 10 in the waste water 5More than the CFU/mL;
D. amplification, blowing air stirs, pH neutrality, temperature and time sees Table 4 amplification condition hurdles;
E. absorption is stirred, pH neutrality, and temperature and time sees Table 4 adsorption conditions hurdles;
F. separate, will obtain cell-oil body mixture through the waste water filtering of hydrophobicity microorganism oil suction processing;
G. measure, record COD value and oil residues content in the processed waste water with extraction process.
For the embodiment 4,16,17 and 19 that uses the dead cell body, nutrient solution is carried out pasteurize handle, under 8000g, obtained bacterium mud in centrifugal 10 minutes again, then bacterium mud is dropped into oily(waste)water and makes bacterium mud weight account for 1% of wastewater flow rate, directly carry out oil body absorption.
Table 2 kind of waste water
Numbering Wastewater source COD Oleaginousness
1 Certain oil refinery effluent 1560mg/L 730mg/L
2 Oil production waste water in oil field after the oil removal 950mg/L 380mg/L
3 Railway locomotive depot waste water 860mg/L 470mg/L
4 Vegetable oil mill waste water 22000mg/L 1200mg/L
5 Vegetable oil mill waste water after the oil removal 16000mg/L 740mg/L
Table 3 hardening and tempering process
Numbering Treatment time Treatment temp Fungistat Concentration
1 2h 60℃ Do not have Do not have
2 24h 30℃ Hydrogen peroxide 0.1％
3 48h 20℃ Peracetic Acid 0.2％
4 24h 30℃ Acidic oxidized electric potential water 10％
5 0h 30℃ Do not have Do not have
6 12h 20℃ Alkaline oxygenated current potential water 5％
Embodiment 1～embodiment 24 uses hydrophobicity microorganism shown in the table 1, and oily(waste)water is handled shown in the his-and-hers watches 2, and the hardening and tempering process that is adopted lists in 3.
As shown in table 4, embodiment 1～embodiment 11 all uses the individual plant microorganism, and wherein embodiment 2,3 and 11 uses yeast, actinomycetes and mould as the hydrophobicity microorganism respectively.And wherein embodiment 4 usefulness dead cells directly adsorb oily(waste)water.
In addition, the two or more hydrophobicity microorganism that embodiment 12～embodiment 24 usefulness make up is at random handled oily(waste)water jointly, and wherein embodiment 16,17 and 19 uses blended hydrophobicity microorganism dead cell.
Table 4 embodiment 1～embodiment 24
Embodiment Waste water Modified Bacterial strain Amplification condition Adsorption conditions Handle back COD Handle the back oleaginousness
1 3 6 5 30℃，48h 30℃，12h 570mg/L 290mg/L
2 5 2 8 30℃，36h 20℃，48h 595mg/L 250mg/L
3 5 2 12 30℃，36h 20℃，48h 600mg/L 312mg/L
4 5 6 30 Use dead cell 15℃，6h 1130mg/L 586mg/L
5 1 2 41 30℃，36h 20℃，12h 476mg/L 177mg/L
6 2 2 42 28℃，36h 20℃，48h 366mg/L 177mg/L
7 2 1 46 30℃，36h 20℃，48h 560mg/L 270mg/L
8 1 3 51 28℃，48h 15℃，8h 580mg/L 220mg/L
9 4 5 51 30℃，36h 20℃，48h 510mg/L 160mg/L
10 5 2 52 30℃，36h 20℃，48h 580mg/L 209mg/L
11 5 2 57 30℃，36h 20℃，48h 560mg/L 326mg/L
12 3 6 1-7 30℃，48h 30℃，12h 410mg/L 211mg/L
13 4 5 6-10 30℃，36h 20℃，48h 1110mg/L 360mg/L
14 5 2 12-15 30℃，36h 20℃，6h 970mg/L 501mg/L
15 4 4 15-19 30℃，36h 20℃，36h 1210mg/L 563mg/L
16 5 3 20-24 Use dead cell 20℃，12h 1800mg/L 313mg/L
17 3 3 25-30 Use dead cell 15℃，8h 207mg/L 85mg/L
18 5 6 28-32 30℃，36h 20℃，36h 880mg/L 533mg/L
19 5 5 33-38 Use dead cell 15℃，6h 2020mg/L 466mg/L
20 5 2 39-40 30℃，36h 20℃，48h 890mg/L 350mg/L
21 1 2 41-45 30℃，36h 20℃，12h 210mg/L 70mg/L
22 2 2 41-47 28℃，36h 20℃，48h 215mg/L 15mg/L
23 2 1 46-50 30℃，36h 20℃，48h 142mg/L 9mg/L
24 1 3 50-56 28℃，48h 15℃，8h 342mg/L 160mg/L
From the result of the listed embodiment 1～embodiment 24 of table 4 as can be seen, handle various types of oily(waste)waters with single strain hydrophobicity microorganism cells and can obviously reduce waste water oleaginousness and COD value; Often can obtain better treatment effect when being used in combination with the various hydrophobic microorganism; And, no matter use single strain or many bacterial strains, directly adsorb oily(waste)water with the dead cell body and also can obtain good deoiling effect.
In addition, cell-oil body mixture of obtaining of the foregoing description is the granular substance or the floss of solid/semi-solid.Filtration obtains can making recycling behind these cells-oil body mixture, comprising: do fermentation culture medium for microbe and use, do oil fuel and use, separate behind the oil body reuse etc.
The COD that looks oily(waste)water is different with oil-containing, and the inventive method can be used as the final handling procedure before the discharge of wastewater, also as the pre-treatment program of other waste water treatment process, to reduce the load of subsequent wastewater treatment system.
In the specific embodiment of the invention, those skilled in the art can optimize the waste water hardening and tempering process according to used hydrophobicity microorganism type and waste water composition, makes to reach better amplification and adsorption effect.Also can be according to the physico-chemical property of waste water, the hydrophobicity microorganism of selecting to optimize makes up, and it can better be increased and adsorb.
Above-mentioned example only is used for setting forth and unrestricted the present invention.Those skilled in the art design is made according to the technology of the present invention change or modification all should be in claim protection domains of the present invention.

Claims (10)

1. the method with the sorbent treatment oily(waste)water is characterized in that, described sorbent material is the hydrophobicity microorganism cells, and comprises,
Adsorption step: the hydrophobicity microorganism cells is dropped into oily(waste)water and mixing, with the oil body in the absorption oily(waste)water; With
Separating step: the cell-oil body mixture that forms in the described adsorption step is separated from oily(waste)water.
2. the method for claim 1 is characterized in that, described hydrophobicity microorganism is selected from: aurantia small bacillus (Exiguobacterium aurantiacum)
Onion bulkholderia cepasea (Burkholderia cepacia)
Pseudomonas stanieri (Pseudomonas stutzeri)
Nocardia bacteria (Nocardiodes sp.)
Rhodococcus (Rhodococcus sp.)
Mycobacterium (Mycobacterium)
Sphingomonas paucimobilis (Sphingomonas paucimobilis var.)
Antarctic candida (Candida antarctica)
Pseudomonas testosteroni (Comamonas testosteroni)
Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans)
Actinomyces naeslundii (Actinomyces naeslundii)
Candida albicans (Candida albicans)
Enterococcus faecalis (Enterococci faecalis)
Lactobacterium acidophilum (Lactobacilli acidophilus)
Lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus)
Lactobacillus fermentum (Lactobacilli fermentum)
Bacterium lacticum (Lactobacilli gasseri)
Lactobacillus Jensenii (Lactobacilli jensenii)
Plant lactobacillus (Lactobacilli plantarum)
Pseudomonas aeruginosa (Pseudomonas aeruginosa)
Staphylococcus epidermidis (Staphylococci epidermidis)
Gall's chain coccus (Streptococci gordonii)
Streptococcus mitis (Streptococci mitis)
Streptococcus oralis (Streptococci oralis)
Streptococcus sanguis (Streptococci sanguis)
Subtilis (Bacillus subtilis)
Maltose candiyeast (Candida maltosa)
Inferior sieve is separated fat yeast (Yarrowia lipolytica)
Rhodococcus (Rhodococcus erythropolis)
Many wrinkle general Salmonellas (Prauserella rugosa)
Red-purple rhodococcus (Rhodococcus rhodochrous)
Sweet pea band rhodococcus (Rhodococcus fascians)
Pseudomonas fluorescens (Pseudomonas fluorescens)
Acinetobacter calcoaceticus (Acineobacter sp.)
Acinetobacter calcoaceticus (Acineobacter calcoaceticus)
Pseudomonas putida (Pseudomonas putida)
Accidental mycobacterium (Mycobacterium fortuitum)
Pleat streptomycete (Streptyomyces plicatus)
Pseudomonas mendocina (Pseudomonas mendocina)
Aspergillus nidulans (Aspergillus nidulans), or their arbitrary combination.
3. the method for claim 1 is characterized in that, described hydrophobicity microorganism is selected from: aurantia small bacillus (Exiguobacterium aurantiacum) NCDO 2321 (T)
Onion bulkholderia cepasea (Burkholderia cepacia) LMG 12614t2
Pseudomonas stanieri (Pseudomonas stutzeri) AN10
Nocardia bacteria (Nocardiodes sp.) KP7
Rhodococcus (Rhodococcus sp.) I24
Mycobacterium (Mycobacterium) PYR-1
Sphingomonas paucimobilis (Sphingomonas paucimobilis var.) EPA505
Antarctic candida (Candida antarctica) T-34
Pseudomonas testosteroni (Comamonas testosteroni) B-356
Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1098
Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans) HG1099
Actinomyces naeslundii (Actinomyces naeslundii) PK29
Candida albicans (Candida albicans) ATCC10261
Enterococcus faecalis (Enterococci faecalis) C1030
Lactobacterium acidophilum (Lactobacilli acidophilus) T13
Lactobacterium acidophilum (Lactobacilli acidophilus) RC14
Lactobacterium acidophilum (Lactobacilli acidophilus) 68
Lactobacterium acidophilum (Lactobacilli acidophilus) 75
Lactobacterium casei rhamnosyl subspecies (Lactobacilli casei subsp.rhamnosus) 81
Lactobacillus fermentum (Lactobacilli fermentum) B54
Bacterium lacticum (Lactobacilli gasseri) 56
Bacterium lacticum (Lactobacilli gasseri) 60
Lactobacillus Jensenii (Lactobacilli jensenii) RC28
Plant lactobacillus (Lactobacilli plantarum) RC20
Pseudomonas aeruginosa (Pseudomonas aeruginosa) AK1
Staphylococcus epidermidis (Staphylococci epidermidis) 3294
Staphylococcus epidermidis (Staphylococci epidermidis) 3081
Staphylococcus epidermidis (Staphylococci epidermidis) 26741
Staphylococcus epidermidis (Staphylococci epidermidis) 26512
Gall's chain coccus (Streptococci gordonii) NCTC7869
Streptococcus mitis (Streptococci mitis) BA
Streptococcus mitis (Streptococci mitis) BMS
Streptococcus mitis (Streptococci mitis) T9
Streptococcus oralis (Streptococci oralis) PK1317
Streptococcus oralis (Streptococci oralis) H1
Streptococcus sanguis (Streptococci sanguis) CR311
Subtilis (Bacillus subtilis) ATCC7058
Subtilis (Bacillus subtilis) ATCC15811
Maltose candiyeast (Candida maltosa) EH60
Inferior sieve is separated fat yeast (Yarrowia lipolytica) EH59
Rhodococcus (Rhodococcus erythropolis) NRRL B-16531
Many wrinkle general Salmonella (Prauserella rugosa) NRRL B-2295
Red-purple rhodococcus (Rhodococcus rhodochrous) NCIMB 12566
Rhodococcus (Rhodococcus erythropolis) ATCC 4277T
Sweet pea band rhodococcus (Rhodococcus fascians) DSM 20669
Pseudomonas fluorescens (Pseudomonas fluorescens) CHA0
Acinetobacter calcoaceticus (Acineobacter sp.) ADP-1
Acinetobacter calcoaceticus (Acineobacter calcoaceticus) ATCC 33304
Rhodococcus (Rhodococcus sp.) Q15
Pseudomonas putida (Pseudomonas putida) ATCC 29347
Accidental mycobacterium (Mycobacterium fortuitum) CGMCC 1.514
Accidental mycobacterium (Mycobacterium fortuitum) CGMCC 1.513
Pleat streptomycete (Streptyomyces plicatus) KCC30
Pleat streptomycete (Streptyomyces plicatus) KCC42
Pseudomonas mendocina (Pseudomonas mendocina) CGMCC 1.1768
Aspergillus nidulans (Aspergillus nidulans) CCIC2198 and their mutant strain or their combination arbitrarily.
4. the method for claim 1 is characterized in that, described hydrophobicity microorganism cells is a hydrophobic value more than or equal to 30% microorganism cells.
5. the method for claim 1 is characterized in that, described oil is mineral oil, vegetable and animals oils or synthetic oil.
6. the method for claim 1 is characterized in that, described adsorption step comprises at least one in following two processes:
The microorganism amplification procedure drops into oily(waste)water with described hydrophobicity microorganism, and mixing, blowing air under the temperature that is fit to this hydrophobicity microorganism growth make this hydrophobicity microorganism cells obtain amplification; With
The microorganism adsorption process is being fit under the temperature of described hydrophobicity microorganism cells oil suction effect, and the mixture of described hydrophobicity microorganism and oily(waste)water is stirred.
7. the method for claim 1 is characterized in that, described adsorption step comprises:
The microorganism amplification procedure: described hydrophobicity microorganism is dropped into oily(waste)water, and stirring, blowing air under 25～40 ℃ of temperature make this hydrophobicity microorganism cells obtain amplification; With
Microorganism adsorption process: behind described microorganism amplification procedure, under 15～40 ℃ of temperature, continue to stir, so that the oil body in the described hydrophobicity microorganism cells absorption oily(waste)water forms microorganism cells-oil body mixture.
8. the method for claim 1 is characterized in that, before described adsorption step, also comprises modified step, and described modified step is: waste water is warming up to more than 40 ℃, perhaps adds fungistat in waste water, to suppress original microbial growth in the waste water.
9. method as claimed in claim 8 is characterized in that, described modified step is: waste water is warming up to 40-70 ℃, kept 0.5-10 hour; The fungistat that perhaps adds 0.01%-10% in waste water was handled 12-48 hour, and described fungistat comprises hydrogen peroxide, Peracetic Acid, acidic oxidized electric potential water, alkaline oxygenated current potential water or its combination; Perhaps be described hyperthermic treatment and described fungistat treatment combination.
10. the method for claim 1 is characterized in that, described hydrophobicity microorganism cells is viable cell or dead cell.
CN2007101688771A 2007-12-13 2007-12-13 Treatment method of oil-containing waste water Expired - Fee Related CN101205101B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2007101688771A CN101205101B (en) 2007-12-13 2007-12-13 Treatment method of oil-containing waste water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2007101688771A CN101205101B (en) 2007-12-13 2007-12-13 Treatment method of oil-containing waste water

Publications (2)

Publication Number Publication Date
CN101205101A true CN101205101A (en) 2008-06-25
CN101205101B CN101205101B (en) 2010-11-24

Family

ID=39565611

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2007101688771A Expired - Fee Related CN101205101B (en) 2007-12-13 2007-12-13 Treatment method of oil-containing waste water

Country Status (1)

Country Link
CN (1) CN101205101B (en)

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101993177A (en) * 2010-10-13 2011-03-30 江苏博大环保股份有限公司 Method for treating polymer-containing sewage of oil field
CN102060350A (en) * 2010-11-18 2011-05-18 中国海洋石油总公司 Deep treatment process of oily wastewater
CN102643748A (en) * 2012-05-09 2012-08-22 刘建伦 Sludge-reduced microorganism combined dominant population in sewage processing
CN102660531A (en) * 2012-04-23 2012-09-12 新疆农业科学院微生物应用研究所 Decomposition maturing agent and application thereof to green waste recycling
CN102852497A (en) * 2012-08-07 2013-01-02 陕西延长石油(集团)有限责任公司研究院 Compound microorganism oil extraction method for low permeability oilfield
CN103045509A (en) * 2012-12-13 2013-04-17 霍泽仁 Application of Comamonas testosterone Link
CN103748214A (en) * 2011-07-13 2014-04-23 生物地带有限公司 Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same
CN104402174A (en) * 2014-11-28 2015-03-11 云南圣清环保科技有限公司 Deep treatment method of coal chemical industry wastewater
CN106635871A (en) * 2016-10-08 2017-05-10 浙江双良商达环保有限公司 Complex bacteria and application thereof
CN107475161A (en) * 2017-09-25 2017-12-15 黄灵君 The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation
CN107557318A (en) * 2017-09-25 2018-01-09 黄灵君 For removing the microorganism formulation of hydrogen sulfide malodorant
CN108164087A (en) * 2017-12-27 2018-06-15 中节能环保装备股份有限公司 A kind of oily waste water multi-stage combination processing method and its device
CN108546653A (en) * 2018-03-12 2018-09-18 浙江亲水园生物科技有限公司 A kind of novel fermentation Bacillus acidi lactici and its application in field of fodder and treated sewage field
CN108996707A (en) * 2018-08-17 2018-12-14 西北工业大学 A kind of polycyclic aromatic hydrocarbon bioremediation agents, preparation method and its application
CN109554303A (en) * 2017-09-27 2019-04-02 中国石油化工股份有限公司 A kind of aurantia Exiguobacterium sp and preparation and its application
CN110157634A (en) * 2019-03-12 2019-08-23 浙江工业大学 It is a kind of to move Sphingol single-cell and its application less
CN112047570A (en) * 2020-09-03 2020-12-08 常德鑫芙蓉环保有限公司 Treatment method of oily industrial wastewater
CN114437954A (en) * 2020-11-03 2022-05-06 中国石油天然气集团有限公司 Bacillus flexus for degrading sulfonated drilling fluid, mixed bacteria and application of bacillus flexus
CN114958685A (en) * 2022-06-24 2022-08-30 太原理工大学 Hg removal method 2+ Aerobic denitrifying strain with performance and application thereof

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101993177A (en) * 2010-10-13 2011-03-30 江苏博大环保股份有限公司 Method for treating polymer-containing sewage of oil field
CN101993177B (en) * 2010-10-13 2013-05-01 江苏博大环保股份有限公司 Method for treating polymer-containing sewage of oil field
CN102060350A (en) * 2010-11-18 2011-05-18 中国海洋石油总公司 Deep treatment process of oily wastewater
CN103748214A (en) * 2011-07-13 2014-04-23 生物地带有限公司 Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same
CN103748214B (en) * 2011-07-13 2016-07-06 生物地带有限公司 For removing the biological product of degradation resistant chemicals in water, industrial wastewater and soil and using the method for these goods
CN102660531A (en) * 2012-04-23 2012-09-12 新疆农业科学院微生物应用研究所 Decomposition maturing agent and application thereof to green waste recycling
CN102643748A (en) * 2012-05-09 2012-08-22 刘建伦 Sludge-reduced microorganism combined dominant population in sewage processing
CN102852497A (en) * 2012-08-07 2013-01-02 陕西延长石油(集团)有限责任公司研究院 Compound microorganism oil extraction method for low permeability oilfield
CN103045509A (en) * 2012-12-13 2013-04-17 霍泽仁 Application of Comamonas testosterone Link
CN104402174A (en) * 2014-11-28 2015-03-11 云南圣清环保科技有限公司 Deep treatment method of coal chemical industry wastewater
CN106635871A (en) * 2016-10-08 2017-05-10 浙江双良商达环保有限公司 Complex bacteria and application thereof
CN107557318B (en) * 2017-09-25 2021-07-20 杭州富阳佳畅机械有限公司 Microbial preparation for removing hydrogen sulfide malodorous substance
CN107475161A (en) * 2017-09-25 2017-12-15 黄灵君 The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation
CN107557318A (en) * 2017-09-25 2018-01-09 黄灵君 For removing the microorganism formulation of hydrogen sulfide malodorant
CN107475161B (en) * 2017-09-25 2021-07-20 杭州富阳佳畅机械有限公司 Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation
CN109554303B (en) * 2017-09-27 2022-04-12 中国石油化工股份有限公司 Microbacterium aurantiacus and preparation and application thereof
CN109554303A (en) * 2017-09-27 2019-04-02 中国石油化工股份有限公司 A kind of aurantia Exiguobacterium sp and preparation and its application
CN108164087B (en) * 2017-12-27 2021-03-26 中节能环保装备股份有限公司 Multi-stage combined treatment method and device for oily wastewater
CN108164087A (en) * 2017-12-27 2018-06-15 中节能环保装备股份有限公司 A kind of oily waste water multi-stage combination processing method and its device
CN108546653A (en) * 2018-03-12 2018-09-18 浙江亲水园生物科技有限公司 A kind of novel fermentation Bacillus acidi lactici and its application in field of fodder and treated sewage field
CN108996707A (en) * 2018-08-17 2018-12-14 西北工业大学 A kind of polycyclic aromatic hydrocarbon bioremediation agents, preparation method and its application
CN108996707B (en) * 2018-08-17 2021-08-17 西北工业大学 Polycyclic aromatic hydrocarbon bioremediation agent, and preparation method and application thereof
CN110157634A (en) * 2019-03-12 2019-08-23 浙江工业大学 It is a kind of to move Sphingol single-cell and its application less
CN110157634B (en) * 2019-03-12 2021-06-04 浙江工业大学 Sphingomonas paucimobilis and application thereof
CN112047570A (en) * 2020-09-03 2020-12-08 常德鑫芙蓉环保有限公司 Treatment method of oily industrial wastewater
CN114437954A (en) * 2020-11-03 2022-05-06 中国石油天然气集团有限公司 Bacillus flexus for degrading sulfonated drilling fluid, mixed bacteria and application of bacillus flexus
CN114958685A (en) * 2022-06-24 2022-08-30 太原理工大学 Hg removal method 2+ Aerobic denitrifying strain with performance and application thereof
CN114958685B (en) * 2022-06-24 2023-09-15 太原理工大学 Hg-removing device 2+ Performance aerobic denitrification strain and application thereof

Also Published As

Publication number Publication date
CN101205101B (en) 2010-11-24

Similar Documents

Publication Publication Date Title
CN101205101B (en) Treatment method of oil-containing waste water
Prieto et al. Degradation of phenol by Rhodococcus erythropolis UPV-1 immobilized on Biolite® in a packed-bed reactor
US20150352610A1 (en) Microbial compositions for hydrocarbon remediation and methods of use thereof
JP6359091B2 (en) Microbial based sewage treatment composition and method of use
Jin et al. Biodegradation of aniline in an alkaline environment by a novel strain of the halophilic bacterium, Dietzia natronolimnaea JQ-AN
Felshia et al. Efficacy of free and encapsulated Bacillus lichenformis strain SL10 on degradation of phenol: a comparative study of degradation kinetics
JP3450518B2 (en) Novel microorganism J1, biodegradation treatment method for aromatic compound and / or organochlorine compound using the same and environmental restoration method
Li et al. Biodegradation of nitrobenzene in a lysogeny broth medium by a novel halophilic bacterium Bacillus licheniformis
Dias-Machado et al. Treatment of cork boiling wastewater using chemical oxidation and biodegradation
WO2006090780A1 (en) Estrogenic substance degradable microorganism and use thereof
Elangovan et al. Performance evaluation of various bioreactors for the removal of Cr (VI) and organic matter from industrial effluent
Suhaila et al. Biotransformation using resting cells of Rhodococcus UKMP-5M for phenol degradation
KR101300348B1 (en) Liquid composition of microorganisms for bioremediation of hydrocarbon-contaminated soil, method of preparing the same, and Bioremediation using the same
US6383797B1 (en) Bacterial consortium EBC1000 and a method using the bacterial consortium EBC1000 for remedying biologically recalcitrant toxic chemicals contained in industrial wastewater, waste materials and soils
Ishaya et al. Degradation of used engine oil by alcaligenes sp. strain isolated from oil contaminated site: isolation, identification, and optimization of the growth parameters
Gaskin et al. Comparison of enrichment methods for the isolation of pyrene-degrading bacteria
RU2509150C2 (en) Association of strains of bacteria-oil decomposers, and remediation method of oil-contaminated objects
US9669437B1 (en) Method for scavenging aromatic hydrocarbons, crude petroleum and/or a petroleum refined product
Amini et al. Biodegradation of polycyclic aromatic hydrocarbons by Pseudomonas species
Omer et al. Biovalorization of Olive Mill Wastewater Using Phenol Degrading Bacteria to Produce Biofertilizer
Cisneros-de La Cueva et al. Isolation and characterization of a novel strain, Bacillus sp KJ629314, with a high potential to aerobically degrade diesel
KR20090030897A (en) Liquid composition of microorganisms for bioremediation of hydrocarbon-contaminated soil, method of preparing the same, and bioremediation using the same
Mohanty Microbial Degradation of Phenol: A Comparitive Study
Sathish Stabilization of bacterial cells culture on immobilized Alginate beads and optimization of Congo red decolorization
RU2237711C1 (en) Method of restoring oil-polluted bleaching earth

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101124

Termination date: 20131213