CN106635871A - Complex bacteria and application thereof - Google Patents
Complex bacteria and application thereof Download PDFInfo
- Publication number
- CN106635871A CN106635871A CN201610878211.4A CN201610878211A CN106635871A CN 106635871 A CN106635871 A CN 106635871A CN 201610878211 A CN201610878211 A CN 201610878211A CN 106635871 A CN106635871 A CN 106635871A
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- CN
- China
- Prior art keywords
- wsh
- pseudomonas aeruginosa
- streptococcus
- bbe
- candida mycoderma
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention discloses complex bacteria and an application thereof, and belongs to the technical field of industrial microorganisms. The complex bacteria provided by the invention comprise pseudomonas aeruginosa, streptococcus and candida; the strains are respectively cultured to achieve that OD600 is equal to 1, in respective corresponding mediums, and then mixing the strains according to a volume ratio of 5:3:2. After the complex bacteria provided by the invention with an inoculation amount of 5-10% is inoculated into grease wastewater for reaction for 48h, a grease degradation rate reaches 90.2%, so that the complex bacteria can be applied to industry in large scale, and has the advantages of small investment, high efficiency and free of secondary pollution.
Description
Technical field
The present invention relates to a kind of composite bacteria agent capable and its application, belong to industrial microbial technology field.
Background technology
In China, water pollution is still the problem of a sternness.Industrial wastewater, urban domestic wastewater discharge capacity are all very big.The whole nation
Subsoil water is polluted by point-like or planar.The industry of oil wastewater main source, agricultural, transport and sanitary wastewater discharge.Oils and fatss
Water surface is covered in, the oxygen blocked in air spreads to water body, so that water body self-purification ability declines, ecological balance meets with water
To destruction.At present.Processing the method for oil wastewater mainly has Physical, chemical method and bioanalysises.Physical is mainly according to water
With oily objectionable intermingling, density difference, both are disperseed using gravity and centrifugal force, such as gravitational separation process, coalescence, filtration is inhaled
Attached method, membrane separation process etc..Chemical method mainly uses chemical substance and carries out chemolysis to the Organic substance in waste water, to useful
Organic substance causes damage, and also easily causes secondary pollution.Common method has chemical demulsification oil removing and chemical oxidation etc..Bioanalysises
Process oil wastewater and mainly use microorganism by grease hydrolysis into glycerol, fatty acid, final decomposition is oxidized to water and drinks titanium dioxide
The products such as carbon.Oils and fatss in Biological Treatment of Wastewater have the advantages of putting into little, efficiency high, non-secondary pollution.
The content of the invention
First purpose of the present invention is to provide a kind of composite bacteria agent capable, including Pseudomonas aeruginosa, streptococcus, false silk ferment
Mother, the microbial inoculum is with 5:3:2 volume ratio will be cultivated respectively to OD600=1～1.2 bacterium solutions are mixed.
In one embodiment of the invention, the composite bacteria agent capable is Pseudomonas aeruginosa BBE by Pseudomonas aeruginosa,
Streptococcus WSH-24 and candida mycoderma WSH-Z06 is constituted.
In one embodiment of the invention, the Pseudomonas aeruginosa BBE, streptococcus WSH-24 and candida mycoderma
WSH-Z06 bacterium are through domestication；The domestication is carried out in domestication culture medium；It is described domestication medium component be：(NH4)2SO40.5g/L, NaCl 0.5g/L, K2HPO40.3g/L, MgSO4·7H2O 0.2g/L, peptone 1.0g/L, 1～4g/ of Oleum Glycines
L。
Second object of the present invention is to provide the preparation method of the microbial inoculum, is by Pseudomonas aeruginosa BBE, streptococcus
WSH-24 and candida mycoderma WSH-Z06 are seeded to respectively culture medium, and it is OD to cultivate under the conditions of 30 DEG C to cell concentration600=
0.1；Then by Pseudomonas aeruginosa BBE, streptococcus WSH-24 and candida mycoderma WSH-Z06 Cell saps with 5:3:2 ratios are mixed
Close.
In one embodiment of the invention, the Pseudomonas aeruginosa BBE is seeded in LB culture medium and is trained
Support.
In one embodiment of the invention, the streptococcus WSH-24 is seeded in potato sucrose culture medium
Row culture.
In one embodiment of the invention, the candida mycoderma WSH-Z06 is seeded to containing yeast powder 5g/L, egg
White peptone 10g/L, is cultivated in the culture medium of anhydrous glucose 20g/L.
The method that third object of the present invention is to provide oils and fatss in a kind of degraded oil wastewater, is added in oil wastewater
Enter the composite bacteria agent capable, 10～60h is reacted at 30 DEG C.
In one embodiment of the invention, the addition of the composite bacteria agent capable is by volume 5～10%.
The present invention also provides application of the microbial inoculum in sewage disposal.
Beneficial effects of the present invention：The invention provides a kind of composite bacteria agent capable, the oil that can effectively degrade in oil wastewater
Fat.The microbial inoculum of the present invention is seeded in oil wastewater and is reacted after 48h, degradation rate is up to 90.2%.
Specific embodiment
The measure of oil and grease degradation rate：
Fat content in reactant liquor is measured using UV-2450 types ultraviolet-visible spectrophotometer.Extract reaction solution
Separatory funnel is proceeded to after 10mL, plus the acidifying of 1.5mL sulphuric acid, 5mL toluene is taken and is added separatory funnel, stratification extraction after concussion.
By the water sample after extraction to returning in former separatory funnel, plus 5mL toluene repeats extraction once.Extract mixing is dilute with toluene
Release, the light absorption value of extract is determined under 324nm wavelength.The dense of oils and fatss can be calculated according to oils and fatss with the standard curve of light absorption value
Degree.
Culture medium：
Domestication culture medium (g/L)：(NH4)2SO40.5, NaCl 0.5, K2HPO40.3, MgSO4·7H2O 0.2, albumen
Peptone 1.0, Oleum Glycines concentration increases to successively 4g/L from 1g/L.
Detection culture medium (g/L)：(NH4)2SO45.0, Na2HPO42.0, K2HPO42.0, NaCl 2.0g, MgSO40.5,
Soybean oil 20mL.
LB culture medium (g/L)：Yeast powder 5, peptone 10, Sodium Chloride 5, for cultivating Pseudomonas aeruginosa.
Potato sucrose culture medium (g/L)：Rhizoma Solani tuber osi 200, sucrose 20, for cultivating streptococcus WSH-24.
Candida mycoderma WSH-Z06 basal mediums (g/L)：Yeast powder 5, peptone 10, anhydrous glucose 20.
The preparation of the composite bacteria agent capable of embodiment 1
1st, the domestication of bacterial strain：By Pseudomonas aeruginosa BBE (deposit number CCTCC NO：M 2011185), streptococcus WSH-
(deposit number is CCTCC NO for 24 (being purchased from ATCC, its numbering is ATCC 35246) and candida mycoderma WSH-Z06：M 20714)
Be seeded to respectively in the domestication culture medium without soybean oil, with 5d as cultivation cycle, tame culture medium in Semen sojae atricolor oil concentration according to
Secondary 1g/L, 2g/L, 3g/L, 4g/L of increasing to is tamed, and the bacterium solution before and after domestication is reacted respectively in detection culture medium, is surveyed
Stand oil fat degradation effect, as a result as shown in table 1.Through domestication, candida mycoderma WSH-Z06 oil and grease degradation rates are improved to 78.2%,
The oil and grease degradation rate of Pseudomonas aeruginosa BBE and streptococcus WSH-24 is respectively increased to 50.1% and 43.9%.
2nd, the Pseudomonas aeruginosa BBE after domestication, streptococcus WSH-24 and candida mycoderma WSH-Z06 bacterium are seeded to respectively
Corresponding basal medium is cultivated so as to OD600=1～1.2.
3rd, by the culture fluid of Pseudomonas aeruginosa BBE, streptococcus WSH-24 and candida mycoderma WSH-Z06 bacterium with 5：3：2
Ratio mixing, prepare composite bacteria agent capable.
The oil and grease degradation rate (%) of bacterial strain before and after 1 three plants of bacterium domestications of table
The composite bacteria agent capable of embodiment 2 degraded oil wastewater
The step of by embodiment 1, prepares composite bacteria agent capable, and its difference is bacterial strain not to be tamed.By microbial inoculum with 5%
Addition is seeded in oil wastewater, and in 30 DEG C 48h is reacted, and calculates degradation rate.As a result show, without microbial inoculum pair prepared by domestication
The degradation rate of oils and fatss reaches 63.5%.
The composite bacteria agent capable of embodiment 3 degraded oil wastewater
Microbial inoculum prepared by embodiment 1 is seeded in oil wastewater respectively with Different adding amount, and 48h, meter are reacted in 30 DEG C
Degradation rate is calculated, as a result as shown in table 2.Microbial inoculum is inoculated with 5～10% volume ratio, oil and grease degradation rate up to more than 80%,
With significant effect.
Oil and grease degradation rate under the different condition of table 2
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclosing should be by being defined that claims are defined.
Claims (10)
1. a kind of composite bacteria agent capable, it is characterised in that including Pseudomonas aeruginosa, streptococcus, candida mycoderma, the microbial inoculum is by institute
State Pseudomonas aeruginosa, streptococcus, candida mycoderma to cultivate respectively to OD600=1～1.2, then with 5:3:2 volume ratio is by bacterium solution
Mixed.
2. composite bacteria agent capable according to claim 1, it is characterised in that by Pseudomonas aeruginosa be Pseudomonas aeruginosa BBE,
Streptococcus WSH-24 and candida mycoderma WSH-Z06 is constituted.
3. microbial inoculum according to claim 2, it is characterised in that by the Pseudomonas aeruginosa BBE, streptococcus WSH-24 and
Candida mycoderma is tamed by WSH-Z06 bacterium；The domestication is carried out in domestication culture medium；The domestication medium component
For：(NH4)2SO40.5g/L, NaCl 0.5g/L, K2HPO40.3g/L, MgSO4·7H2O 0.2g/L, peptone 1.0g/L, bean
1～4g/L of oil.
4. the preparation method of microbial inoculum described in claim 1 or 2, it is characterised in that by Pseudomonas aeruginosa BBE, streptococcus WSH-
24 and candida mycoderma WSH-Z06 are seeded to respectively culture medium, and it is OD to cultivate under the conditions of 30 DEG C to cell concentration600=0.1；So
Afterwards by Pseudomonas aeruginosa BBE, streptococcus WSH-24 and candida mycoderma WSH-Z06 Cell saps with 5:3:2 ratios mix.
5. method according to claim 4, it is characterised in that the Pseudomonas aeruginosa BBE is seeded to into LB culture medium
In cultivated.
6. method according to claim 4, it is characterised in that the streptococcus WSH-24 is seeded to into potato sucrose training
Cultivated in foster base.
7. method according to claim 4, it is characterised in that the candida mycoderma WSH-Z06 is seeded to containing yeast
Powder 5g/L, peptone 10g/L, are cultivated in the culture medium of anhydrous glucose 20g/L.
8. in a kind of degraded oil wastewater oils and fatss method, it is characterised in that add in oil wastewater multiple described in claim 1
Microbial inoculum is closed, 10～60h is reacted at 30 DEG C.
9. method according to claim 3, it is characterised in that the addition of the composite bacteria agent capable by volume for 5～
10%.
10. application of the arbitrary microbial inoculum of claim 1 or 2 in sewage disposal.
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| CN201610878211.4A CN106635871B (en) | 2016-10-08 | 2016-10-08 | Complex microbial inoculant and application thereof |
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| CN201610878211.4A CN106635871B (en) | 2016-10-08 | 2016-10-08 | Complex microbial inoculant and application thereof |
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| CN106635871B CN106635871B (en) | 2020-02-04 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108359617A (en) * | 2017-12-29 | 2018-08-03 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization |
| CN108823141A (en) * | 2018-06-11 | 2018-11-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of oil smoke rubbish microbiological garbage treatment agent |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN109666602A (en) * | 2018-12-19 | 2019-04-23 | 中国石油天然气集团有限公司 | Oily wastewater degradation compound bacteria and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108359617A (en) * | 2017-12-29 | 2018-08-03 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization |
| CN108359617B (en) * | 2017-12-29 | 2021-03-02 | 浙江双良商达环保有限公司 | Acinetobacter CL05 and application thereof in village and town sewage dephosphorization treatment |
| CN108823141A (en) * | 2018-06-11 | 2018-11-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of oil smoke rubbish microbiological garbage treatment agent |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN109666602A (en) * | 2018-12-19 | 2019-04-23 | 中国石油天然气集团有限公司 | Oily wastewater degradation compound bacteria and preparation method thereof |
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| CN106635871B (en) | 2020-02-04 |
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Address after: 9 floor, block A, Lianhua business center, Lianhua street, Lianhua, Hangzhou,, Xihu District, Zhejiang Patentee after: Zhe JIANG Shuangliang Sunda Environment Protection Co.,Ltd. Address before: 9 floor, block A, Lianhua business center, Lianhua street, Lianhua, Hangzhou,, Xihu District, Zhejiang Patentee before: ZHEJIANG SHUANGLIANG SHANGDA ENVIRONMENTAL PROTECTION Co.,Ltd. |