CN109464375A - A kind of pet deodorant and its preparation method and application - Google Patents

A kind of pet deodorant and its preparation method and application Download PDF

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Publication number
CN109464375A
CN109464375A CN201811513721.7A CN201811513721A CN109464375A CN 109464375 A CN109464375 A CN 109464375A CN 201811513721 A CN201811513721 A CN 201811513721A CN 109464375 A CN109464375 A CN 109464375A
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pet
bacteria
pet deodorant
deodorant
auxiliary material
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黄政雄
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Shenzhen Hemin Biotechnology Co Ltd
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Shenzhen Hemin Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/20Halogens; Compounds thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728Fungi, e.g. yeasts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q15/00Anti-perspirants or body deodorants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cosmetics (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of pet deodorants and its preparation method and application.The pet deodorant is gained after each component mixed fermentation by following mass percent: photosynthetic bacteria 50~60%, saccharomycete 20~30%, lactic acid bacteria 5~15%, brown sugar 1~5%, sea salt 0.5~1.0%, surplus auxiliary material.For pet deodorant provided by the present invention by the synergistic effect between each bacterial strain, pet skin dirt grease of fundamentally degrading makes its fur keep automatically cleaning and free from extraneous odour;The pet deodorant can also inhibit the growth of harmful bacteria with pet, play the double effects of disinfection and deodorization, and to the non-stimulated toxic action of pet itself, can play good health-care effect.The preparation method simple process and low cost of the pet deodorant is suitble to batch production fermenting and producing and popularization and application.

Description

A kind of pet deodorant and its preparation method and application
Technical field
The present invention relates to microbial bacterial agent technical fields, and in particular, to a kind of pet deodorant and preparation method thereof and Using.
Background technique
Current many families all pet-feedings are accompanied as spirit, daily closely related with people.Due to pet cat, dog Etc. no sweat gland, resisting bacterium and other germs will lean on the hair of itself and skin to secret out of a kind of secretory substance with grease Dirt will be generated between this secretory substance long-term existence skin and hair by forming protective film, and give out peculiar smell (fishy smell), With the raising smell more enriching of temperature and humidity, especially rainy day.Further, since accumulation of mud on skin, it is easy to make Bacteria breed causes pet bacterial dermatosis.Therefore, the regular disinfecting, deodorizing of pet just seems particularly significant.
Pet disinfection deodorizer currently on the market, mostly with chlorine class preparation (including inorganic chloride, such as 84 disinfections Agent, sodium hypochlorite, bleaching powder;Organochlorine compound, such as sodium dichloro cyanurate, sodium trichloro-isocyanurate) and aromatic be It is main.If selecting irritation disinfectant, close contact disinfectant when love dotes on smelling is easy in sucking alveolar, will lead to chronic Damage.And cat love licks and licks hair, be infected with thimerosal ingredient just have the possibility licked.Cat and dog are different from mankind's meeting Selection is far from harmful disinfectant, so requirement of the cat and dog to disinfectant is than people Geng Gao, general aromatic or conventional deodorizers For pet deodorization and improper.Therefore, now need to study a kind of safe and nontoxic, fundamentally deodorization biological deodorant.
Summary of the invention
The purpose of the invention is to overcome the above-mentioned deficiency of the prior art, a kind of pet deodorant is provided, which goes The dirt grease that taste agent can effectively degrade on pet skin, and inhibit the growth of harmful bacteria, while reaching deodorization and sterilization Effect.
Another object of the present invention is to provide the preparation methods of above-mentioned pet deodorant.
Another object of the present invention is to provide application of the above-mentioned pet deodorant in degradation grease.
Another object of the present invention is to provide above-mentioned pet deodorants to inhibit the application in bacterial growth.
To achieve the goals above, the present invention is achieved by following scheme:
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: photosynthetic bacteria 50~60%, Saccharomycete 20~30%, lactic acid bacteria 5~15%, brown sugar 1~5%, sea salt 0.5~1.0%, surplus auxiliary material.
Part strain is successfully cultivated, is tamed by test of many times according to the analysis of sources of odor by the present invention, sieve Select the bacterial strain of special efficacy degradation grease and foul smell.Wherein, on the one hand photosynthetic bacteria can degrade foul smell molecule, such as hydrogen sulfide, ammonia Gas and indoles etc., to realize the purpose of removal stink;Another aspect photosynthetic bacteria adaptability itself is extremely strong, can dislike having It is lived and reproduced in the environment of stink, and can effectively inhibit the growth of putrefactive microorganisms, terminate the progress of decay process.Saccharomycete can Energy and effective nutriment (such as protein) required for being provided for other effective biological flora proliferation in pet deodorant, To promote the normal growth of other bacterial strains to be proliferated and maintain normal activities.Lactic acid bacteria can be by the grease on animal skin surface It is metabolized for own growth, small molecule metabolites is broken down into using biological enzyme, to eliminate sources of odor.
In order to make above-mentioned multiple-microorganism flora maintain a relatively steady state, every kind of flora is allowed also for Special role and the interaction relationship between them, the present invention utilize special fermentation after mixing each bacterial strain according to a certain percentage Technique, generate a large amount of biological enzyme, it can be achieved that efficiency maximize, release largely go the peculiar smell factor when in use, effectively drop The dirt grease on pet skin is solved, and inhibits the growth of harmful bacteria, while achieving the effect that deodorization and sterilization, from the root It solves peculiar smell, and enhances the resistance of pet skin.It needs to be emphasized that above-mentioned multiple-microorganism flora is according to one Certainty ratio is compound, moreover it is possible to so that various microbial floras mutually provide energy and original on the basis of playing own biological function Material, shows to act synergistically, and forms a microorganism stable state, and this has very the comprehensive deodorization ability for improving pet deodorant Big facilitation.
Preferably, the pet deodorant is gained after each component mixed fermentation by following mass percent: photosynthetic thin Bacterium 55~60%, saccharomycete 20~25%, lactic acid bacteria 10~15%, brown sugar 1~5%, sea salt 0.5~1.0%, surplus auxiliary material.
It is highly preferred that the pet deodorant is gained after each component mixed fermentation by following mass percent: photosynthetic Bacterium 60%, saccharomycete 20%, lactic acid bacteria 10%, brown sugar 5%, sea salt 0.5%, surplus auxiliary material.
Wherein, the auxiliary material is water, vitamin, amino acid etc..
Preferably, the photosynthetic bacteria includes but is not limited to Rhodopseudomonas palustris, Rhodopseudomonas spheroides, dark red red spiral shell At least one of bacterium.
Preferably, the saccharomycete includes but is not limited at least one of saccharomyces cerevisiae, torulopsis, Candida.
Preferably, the lactic acid bacteria includes but is not limited to lactobacillus acidophilus, streptococcus lactis, at least one in lactobacillus plantarum Kind.
The present invention has played significant synergistic effect, composite bacteria liquid hair by mixing above each bacterial strain according to a certain percentage After ferment for single strain, there is better degradation effect to skin oil and fat, and also obviously mention to the inhibiting rate of dermatophyte It is high.
The preparation method of above-mentioned pet deodorant includes the following steps: respectively to match photosynthetic bacteria, saccharomycete and lactic acid bacteria It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get composite bacteria liquid in proportion;To Brown sugar, sea salt and surplus auxiliary material are added in composite bacteria liquid, is issued in 15~40 DEG C, pH 5.7~6.8,180~200rpm condition Up to pet deodorant after ferment 10~22 days.
Preferably, the fermentation condition is 37 DEG C, pH6.5,190rpm, and fermentation time is 20 days.
As a kind of selectable specific embodiment, the pet deodorant the preparation method is as follows:
S1. first each bacterium solution is added by the above proportion into fermenter in 1000 kilograms of calculation of capacity at cost, in every kind of bacteria suspension Living bacteria count is 107~108Cfu/mL is added the qualified natural water without any chemical substance examined, stirs evenly;
S2. it covers that sealing cover is totally-enclosed, stays tracheae to be inserted in the bottle that 2000mL water is filled in periphery, generate during the fermentation Gas is sprayed by plastic tube toward the bottle for filling water, has a large amount of bubble, can naturally have gas ejection in this process, simultaneously Prevent outside bacterium from entering;Fermentation condition is 15~40 DEG C, pH 5.7~6.8,180~200rpm, and all kinds of strain quantity are being sent out It is constantly proliferated during ferment;
S3. foam can be generated by 5~6 days by fermenting, and be changed from small to big, and can slowly be disappeared to 10 days or so foams, as foam disappears One layer of white suspended matter is had on mistake face, this is saccharomycete effect, and whole process is completed to need 20 days or so to go to get pet Taste agent.
Resulting pet deodorant has a kind of denseer sweet tea sour taste, and pH value is less than 3.8.
Through detecting, the pet deodorant is 60% or so to the degradation rate of skin oil and fat, the inhibiting rate to bacterium is 90~ 98%.Therefore, application of the pet deodorant in degradation grease and inhibition bacterial growth is also claimed in the present invention.
In addition, spraying, degradation of the discovery to ammonia in spraying or air in source of odor using the pet deodorant stoste Rate is 93% or so, and the degradation rate to hydrogen sulfide gas is 92.5% or so, shows that the pet deodorant can effectively remove peculiar smell.
Compared with prior art, the invention has the following advantages:
Pet deodorant provided by the present invention is by the synergistic effect between each bacterial strain, pet skin dirt of fundamentally degrading Grease makes its fur keep automatically cleaning and free from extraneous odour;The pet deodorant can also inhibit the growth of harmful bacteria with pet, The double effects of disinfection and deodorization are played, and to the non-stimulated toxic action of pet itself, good health-care effect can be played.It is described The preparation method simple process and low cost of pet deodorant is suitble to batch production fermenting and producing and popularization and application.
Specific embodiment
The present invention is made combined with specific embodiments below and further being elaborated, the embodiment is served only for explaining this Invention, is not intended to limit the scope of the present invention.Test method as used in the following examples is normal unless otherwise specified Rule method;Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment pet deodorant and comparative example composite bacteria agent are detected to animal skin oil by skin oil and fat test equipment The degradation situation of rouge.
By colony counting method Statistics Implementation example pet deodorant and comparative example composite bacteria agent to animal bacteria dermatoses Pathogen staphylococcus aureus and streptococcic bacteriostasis rate.
It is detected by " the measurement Berthelot spectrophotometry of air and wasteair ammonia " (HJ533-2009) method Embodiment pet deodorant and comparative example composite bacteria agent eliminate situation to particular space ammonia.
Pass through " the measurement gas chromatography of air quality hydrogen sulfide, methyl mercaptan, methyl sulfide and methyl disulfide " (GB/ T14678-1993) method detection embodiment pet deodorant and comparative example composite bacteria agent eliminate situation to particular space hydrogen sulfide.
Embodiment 1
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 40%, Rhodospirillum rubrum 20%, saccharomyces cerevisiae 10%, Candida 10%, lactobacillus acidophilus 4%, lactobacillus plantarum 6%, brown sugar 5%, sea Salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 37 DEG C, pH 6.5,190rpm Up to pet deodorant after it.
Embodiment 2
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 35%, Rhodopseudomonas spheroides 20%, saccharomyces cerevisiae 15%, torulopsis 10%, lactobacillus acidophilus 5%, streptococcus lactis 5%, brown sugar 4%, Sea salt 1.0%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 37 DEG C, pH 6.5,190rpm Up to pet deodorant after it.
Embodiment 3
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas spheroides 20%, Rhodospirillum rubrum 30%, torulopsis 20%, Candida 10%, streptococcus lactis 5%, lactobacillus plantarum 5%, brown sugar 5%, sea salt 0.6%, surplus auxiliary material complements to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 37 DEG C, pH 6.5,190rpm Up to pet deodorant after it.
Embodiment 4
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 30%, Rhodospirillum rubrum 20%, saccharomyces cerevisiae 15%, Candida 10%, lactobacillus acidophilus 5%, lactobacillus plantarum 10%, brown sugar 3%, Sea salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 35 DEG C, pH 6.3,180rpm Up to pet deodorant after it.
Embodiment 5
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 30%, Rhodopseudomonas spheroides 30%, saccharomyces cerevisiae 15%, Candida 15%, lactobacillus acidophilus 4%, lactobacillus plantarum 6%, brown sugar 5%, sea salt 1.0%, surplus auxiliary material complements to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 18 under the conditions of 15 DEG C, pH5.7,180rpm Up to pet deodorant after it.
Embodiment 6
A kind of pet deodorant is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas spheroides 25%, Rhodospirillum rubrum 35%, torulopsis 10%, Candida 15%, lactobacillus acidophilus 5%, lactobacillus plantarum 5%, brown sugar 1%, sea Salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned pet deodorant, comprising the following steps: match photosynthetic bacteria, saccharomycete and lactic acid bacteria respectively It is set to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 40 DEG C, pH 6.8,200rpm Up to pet deodorant after it.
Comparative example 1
A kind of composite bacteria agent is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 25%, Rhodospirillum rubrum 20%, saccharomyces cerevisiae 5%, Candida 10%, lactobacillus acidophilus 2%, lactobacillus plantarum 2%, brown sugar 5%, sea salt 0.5%, surplus auxiliary material complements to 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned composite bacteria agent, comprising the following steps: photosynthetic bacteria, saccharomycete and lactic acid bacteria are respectively configured At bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get composite bacteria liquid according to the above ratio; Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 days under the conditions of 37 DEG C, pH 6.5,190rpm Afterwards to obtain the final product.
Comparative example 2
A kind of composite bacteria agent, be gained after each component mixed fermentation by following mass percent: bacillus subtilis 62% is made Brewer yeast 10%, Candida 10%, lactobacillus acidophilus 4%, lactobacillus plantarum 6%, brown sugar 2%, sea salt 2.0%, surplus auxiliary material are supplied To 100%.Wherein, auxiliary material is water, vitamin, amino acid etc..
The preparation method of above-mentioned composite bacteria agent, comprising the following steps: distinguish bacillus subtilis, saccharomycete and lactic acid bacteria It is configured to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 37 DEG C, pH 6.5,190rpm After it to obtain the final product.
Comparative example 3
A kind of composite bacteria agent is gained after each component mixed fermentation by following mass percent: Rhodopseudomonas palustris 30%, Rhodopseudomonas spheroides 30%, Rhodospirillum rubrum 30%, brown sugar 5%, sea salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material For water, vitamin, amino acid etc..
The preparation method of above-mentioned composite bacteria agent, comprising the following steps: by Rhodopseudomonas palustris, Rhodopseudomonas spheroides and Rhodospirillum rubrum is each configured to bacteria suspension, and living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then according to the above ratio It is mixed to get composite bacteria liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, in 37 DEG C, pH 6.5, After fermenting 20 days under the conditions of 190rpm to obtain the final product.
Comparative example 4
A kind of composite bacteria agent, be gained after each component mixed fermentation by following mass percent: saccharomyces cerevisiae 30%, ball intend ferment Mother 30%, Candida 30%, brown sugar 5%, sea salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, vitamin, ammonia Base acid etc..
The preparation method of above-mentioned composite bacteria agent, comprising the following steps: distinguish saccharomyces cerevisiae, torulopsis and Candida It is configured to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then it is mixed to get compound bacteria according to the above ratio Liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, ferments 20 under the conditions of 37 DEG C, pH 6.5,190rpm After it to obtain the final product.
Comparative example 5
A kind of composite bacteria agent is gained after each component mixed fermentation by following mass percent: lactobacillus acidophilus 30%, newborn chain Coccus 30%, lactobacillus plantarum 30%, brown sugar 5%, sea salt 0.5%, surplus auxiliary material complement to 100%.Wherein, auxiliary material is water, dimension life Element, amino acid etc..
The preparation method of above-mentioned composite bacteria agent, comprising the following steps: by lactobacillus acidophilus, streptococcus lactis and lactobacillus plantarum It is each configured to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, then be mixed to get according to the above ratio multiple Combined bacteria liquid;Brown sugar, sea salt and surplus auxiliary material is added into composite bacteria liquid in proportion, is issued in 37 DEG C, pH 6.5,190rpm condition After ferment 20 days to obtain the final product.
Application examples
Degradation of the composite bacteria agent of the pet deodorant and comparative example 1~5 that detect Examples 1 to 6 respectively to animal skin grease Situation, to staphylococcus aureus and streptococcic bacteriostasis rate, situation is eliminated to particular space ammonia, to particular space hydrogen sulfide Situation is eliminated, concrete outcome is shown in Table 1.
1 sources of odor eradicating efficacy of table and fungistatic effect statistical conditions
The above result shows that the effect of Examples 1 to 6 pet deodorant is substantially better than comparative example 1~5, use is of the present invention Pet deodorant can effectively degrade animal skin dirt grease, solve sources of odor from the root, and can effectively inhibit skin harmful The growth of germ enhances the resistance of pet skin.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all of the invention Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle Within the scope of.

Claims (10)

1. a kind of pet deodorant, which is characterized in that be gained after each component mixed fermentation by following mass percent: photosynthetic Bacterium 50~60%, saccharomycete 20~30%, lactic acid bacteria 5~15%, brown sugar 1~5%, sea salt 0.5~1.0%, surplus auxiliary material.
2. pet deodorant according to claim 1, which is characterized in that the pet deodorant is by following mass percent Each component mixed fermentation after gained: photosynthetic bacteria 55~60%, saccharomycete 20~25%, lactic acid bacteria 10~15%, brown sugar 1~5%, Sea salt 0.5~1.0%, surplus auxiliary material.
3. pet deodorant according to claim 1, which is characterized in that the pet deodorant is by following mass percent Each component mixed fermentation after gained: photosynthetic bacteria 60%, saccharomycete 20%, lactic acid bacteria 10%, brown sugar 5%, sea salt 0.5%, surplus is auxiliary Material.
4. pet deodorant according to claim 1, which is characterized in that the photosynthetic bacteria is Rhodopseudomonas palustris, ball At least one of shape red pseudomonas, Rhodospirillum rubrum.
5. pet deodorant according to claim 1, which is characterized in that the saccharomycete is saccharomyces cerevisiae, torulopsis, vacation At least one of silk yeast.
6. pet deodorant according to claim 1, which is characterized in that the lactic acid bacteria be lactobacillus acidophilus, streptococcus lactis, At least one of lactobacillus plantarum.
7. the preparation method of any one of the claim 1~6 pet deodorant, which comprises the steps of: by light It closes bacterium, saccharomycete and lactic acid bacteria and is each configured to bacteria suspension, living bacteria count is 10 in every kind of bacteria suspension7~108Cfu/mL, It is mixed to get composite bacteria liquid in proportion again;Brown sugar, sea salt and surplus auxiliary material are added into composite bacteria liquid, in 15~40 DEG C, pH 5.7~6.8, up to pet deodorant after fermenting 10~22 days under the conditions of 180~200rpm.
8. preparation method according to claim 7, which is characterized in that the fermentation condition be 37 DEG C, pH 6.5,190rpm, Fermentation time is 20 days.
9. application of any one of the claim 1~6 pet deodorant in degradation grease.
10. any one of the claim 1~6 pet deodorant is inhibiting the application in bacterial growth.
CN201811513721.7A 2018-12-11 2018-12-11 A kind of pet deodorant and its preparation method and application Pending CN109464375A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111500490A (en) * 2020-04-16 2020-08-07 深圳合民生物科技有限公司 Microbial type bacteriostatic and odor-removing preparation as well as preparation method and application thereof

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