CN106520631A - Complex microbial inoculant and application of composite bactericide to sewage treatment - Google Patents
Complex microbial inoculant and application of composite bactericide to sewage treatment Download PDFInfo
- Publication number
- CN106520631A CN106520631A CN201611151975.XA CN201611151975A CN106520631A CN 106520631 A CN106520631 A CN 106520631A CN 201611151975 A CN201611151975 A CN 201611151975A CN 106520631 A CN106520631 A CN 106520631A
- Authority
- CN
- China
- Prior art keywords
- inoculation
- grease
- composite bacteria
- bacteria agent
- yarrowia lipolytica
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
Abstract
The invention discloses complex microbial inoculant and application of the complex microbial inoculant to sewage treatment and belongs to the technical field of industrial microorganisms. The complex microbial inoculant includes swamp rhodop seudanonas palustris, bacillus pumilus and yarrowia lipolytica active ingredients. The complex microbial inoculant is inoculated into grease waste water according to the inoculating amount of 10%-15% to be reacted for 72 h at the temperature of 37 DEG C, and the grease degrading rate of 70% or above can be achieved. After the complex microbial inoculant is inoculated into the grease waste water to be reacted for 72 h, the grease degrading rate reaches 96.8 %, an ideal effect is achieved in pilot magnification of 1 m<3> as well, and the potential for large-scale industrial application is achieved.
Description
Technical field
The present invention relates to a kind of composite bacteria agent and its application in sewage disposal, belong to industrial microbial technology field.
Background technology
In China, water pollution is still a severe problem.Industrial wastewater, urban domestic wastewater discharge capacity are all very big.The whole nation
Underground water is polluted by point-like or planar.The industry of oil wastewater main source, agricultural, transport and sanitary wastewater discharge.Grease
Water surface is covered in, the oxygen blocked in air is spread to water body, so that water body self-purification ability declines, the ecological balance meets with water
To destruction.At present.The method for processing oil wastewater mainly has Physical, chemical method and bioanalysis.Physical is mainly according to water
With oily objectionable intermingling, density difference, both are disperseed using gravity and centrifugal force, such as gravitational separation process, coalescence, filtration is inhaled
Attached method, membrane separation process etc..Chemical method mainly uses chemical substance and carries out chemical breakdown to the organic matter in waste water, to useful
Organic substance causes damage, and also easily causes secondary pollution.Common method has chemical demulsification oil removing and chemical oxidation etc..Bioanalysis
Processing oil wastewater and microorganism being mainly used by grease hydrolysis into glycerine, aliphatic acid, final decomposition is oxidized to water and drinks titanium dioxide
The products such as carbon.Grease in Biological Treatment of Wastewater has the advantages of putting into little, efficiency high, non-secondary pollution.
The content of the invention
First purpose of the present invention is to provide a kind of composite bacteria agent for sewage disposal, and the microbial inoculum is active bio
Product, its active component include Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica.
In one embodiment of the invention, the microbial inoculum is prepared as follows obtaining：By Rhodopseudomonas palustris,
Bacillus pumilus and Yarrowia lipolytica are cultivated respectively to OD600=1.2～1.5, according to 1:1.5～2:1.5～2 ratio,
Culture medium sealing is placed in 37 DEG C after inoculation in fermentation medium by total inoculum concentration co-inoculation of 10～15% volume ratios, quiet
Put fermentation 3～5 days；The fermentation medium components be 50～70g/L of corn steep liquor, urea 1.0%～1.5%, calcium chloride 0.5～
1.5g/L, 0.3～0.6g/L of phosphopyridoxal pyridoxal phosphate.
In one embodiment of the invention, the Yarrowia lipolytica in the microbial inoculum is Yarrowia lipolytica
CGMCC2.1405。
Second object of the present invention is to provide the preparation method of the microbial inoculum, is by Rhodopseudomonas palustris, short and small bud
Spore bacillus and Yarrowia lipolytica are cultivated respectively to OD600=1.2～1.5, according to 1:1.5～2:1.5～2 ratio, 10
Culture medium sealing in fermentation medium is placed in 37 DEG C after inoculation, is stood by total inoculum concentration co-inoculation of～15% volume ratio
Fermentation 3～5 days.
In one embodiment of the invention, the fermentation medium components be 50～70g/L of corn steep liquor, urea 10～
15g/L, 0.5～1.5g/L of calcium chloride, 0.3～0.6g/L of phosphopyridoxal pyridoxal phosphate.
Third object of the present invention is to provide a kind of sewage water treatment method, is with 5～10% by described composite bacteria agent
Volume ratio is put into into sewage, 35～37 DEG C of 24～72h of reaction.
The present invention also provides application of the microbial inoculum in sewage disposal.
Beneficial effect：The invention provides a kind of composite bacteria agent, the grease that effectively can be degraded in oil wastewater.By this
Bright microbial inoculum is seeded to after reacting 72h in oil wastewater, oil and grease degradation rate up to 96.8%, in 1m3Enlarged experiment in also obtain
Preferable effect, possesses the potentiality of large-scale industrial application.
Specific embodiment
Culture medium：
Rhodopseudomonas palustris culture medium：Yeast extract l.5g/L, peptone 2.0g/L, NaCl 0.5g/L, KH2PO4
0.6g/L,K2HPO4 0.9g/L。
Yarrowia lipolytica culture medium：Sucrose 1g/L, peptone 25g/L, urea 1g/L, magnesium sulfate 1g/L, sodium chloride
15g/L, Tween-80 1ml/L.
Bacillus pumilus culture medium：Soybean protein 15g/L, brown sugar 20g/L, ammonium sulfate 10g/L.
The measure of oil and grease degradation rate：
Fat content in reactant liquor is measured using UV-2450 types ultraviolet-visible spectrophotometer.Extract reaction solution
Separatory funnel is proceeded to after 10mL, plus the acidifying of 1.5mL sulfuric acid, 5mL toluene is taken and is added separatory funnel, stratification extraction after concussion.
By the water sample after extraction in time former separatory funnel, plus 5mL toluene repeats extraction once.Will be extract mixing dilute with toluene
Release, the light absorption value of extract is determined under 324nm wavelength.The dense of grease can be calculated with the calibration curve of light absorption value according to grease
Degree.
Embodiment 1
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1：1 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermented and cultured
In base (corn steep liquor 50g/L, urea 15g/L, magnesium chloride 1.0g/L), culture medium sealing is placed in into 37 DEG C after inoculation, standing for fermentation 3
～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 69.7%.
Embodiment 2
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1：1 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermented and cultured
In base (corn steep liquor 50g/L, urea 15g/L, calcium chloride 1.0g/L), culture medium sealing is placed in into 37 DEG C after inoculation, standing for fermentation 3
～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 75.3%.
Embodiment 3
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1：1 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermented and cultured
In base (corn steep liquor 50g/L, urea 15g/L, phosphopyridoxal pyridoxal phosphate 0.6g/L), culture medium sealing is placed in into 37 DEG C after inoculation, is stood
Fermentation 3～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 72.6%.
Embodiment 4
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1：1 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermented and cultured
Base (corn steep liquor 50, urea 15g/L, calcium chloride 1.0g/L, phosphopyridoxal pyridoxal phosphate 0.6g/L.) in, culture medium sealing is put after inoculation
In 37 DEG C, standing for fermentation 3～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 90.3%.
Embodiment 5
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1.5：1.5 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermentation
Culture medium (corn steep liquor 50, urea 15g/L, calcium chloride 1.0g/L, phosphopyridoxal pyridoxal phosphate 0.6g/L.) in, it is culture medium is close after inoculation
37 DEG C are sealed and placed in, standing for fermentation 3～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 95.4%.
Embodiment 6
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：2：2 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermented and cultured
Base (corn steep liquor 50, urea 15g/L, calcium chloride 1.0g/L, phosphopyridoxal pyridoxal phosphate 0.6g/L.) in, culture medium sealing is put after inoculation
In 37 DEG C, standing for fermentation 3～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 96.8%.
Embodiment 7
(1) preparation of microbial inoculum：By Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica respectively respective
Cultivate in culture medium to OD600=1.2, according to 1：1.5：2 ratio, the inoculum concentration co-inoculation of 15% volume ratio are in fermentation training
Foster base (corn steep liquor 50, urea 15g/L, calcium chloride 1.0g/L, phosphopyridoxal pyridoxal phosphate 0.6g/L.) in, culture medium is sealed after inoculation
37 DEG C are placed in, standing for fermentation 3～5 days.
(2) degraded sewage grease：Composite bacteria agent prepared by step (1) is put into useless to 500mL greases with 10% volume ratio
In water (containing grease 40%～50%), in 37 DEG C of incubators, 72h is reacted.
Oil in Sewage Water fat content after and reaction 72h initial to reaction is detected, is as a result shown, grease after reaction 72h
Degradation rate is 95.2%.
Embodiment 8
Microbial inoculum is prepared as described in Example 6, and microbial inoculum is put into 1m by 10% volume ratio3The treatment tank of scale
In, as a result 37 DEG C of reaction 72h show, oil and grease degradation rate is 95.8% after reaction 72h.
Although the present invention is disclosed as above with preferred embodiment, which is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclosing should be by being defined that claims are defined.
Claims (7)
1. a kind of composite bacteria agent for sewage disposal, it is characterised in that the microbial inoculum is active biological product, its active component
Including Rhodopseudomonas palustris, bacillus pumilus and Yarrowia lipolytica.
2. composite bacteria agent according to claim 1, it is characterised in that be prepared as follows obtaining：Will be marsh red false single
Born of the same parents bacterium, bacillus pumilus and Yarrowia lipolytica are cultivated respectively to OD600=1.2～1.5, according to 1:1.5～2:1.5～2
Ratio, 10～15% total inoculum concentration co-inoculation in fermentation medium, after inoculation by culture medium sealing be placed in 37 DEG C, stand
Fermentation 3～5 days；The fermentation medium components are 50～70g/L of corn steep liquor, 10～15g/L of urea, 0.5～1.5g/ of calcium chloride
L, 0.3～0.6g/L of phosphopyridoxal pyridoxal phosphate.
3. composite bacteria agent according to claim 1 and 2, it is characterised in that the Yarrowia lipolytica is solution fat Ye Shi ferment
Female CGMCC 2.1405.
4. the preparation method of composite bacteria agent described in claim 1 or 2, it is characterised in that by Rhodopseudomonas palustris, short and small gemma
Bacillus and Yarrowia lipolytica are cultivated respectively to OD600=1.2～1.5, according to 1:1.5～2:1.5～2 ratio, 10～15%
Total inoculum concentration co-inoculation in fermentation medium, after inoculation by culture medium sealing be placed in 37 DEG C, standing for fermentation 3～5 days.
5. method according to claim 4, it is characterised in that the fermentation medium components be 50～70g/L of corn steep liquor,
10～15g/L of urea, 0.5～1.5g/L of calcium chloride, 0.3～0.6g/L of phosphopyridoxal pyridoxal phosphate.
6. a kind of sewage water treatment method, it is characterised in that the composite bacteria agent described in claim 1 is thrown with 5～10% volume ratios
Enter into sewage, 35～37 DEG C of 24～72h of reaction.
7. application of the microbial inoculum described in claim 1 or 2 in sewage disposal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151975.XA CN106520631A (en) | 2016-12-14 | 2016-12-14 | Complex microbial inoculant and application of composite bactericide to sewage treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151975.XA CN106520631A (en) | 2016-12-14 | 2016-12-14 | Complex microbial inoculant and application of composite bactericide to sewage treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106520631A true CN106520631A (en) | 2017-03-22 |
Family
ID=58339380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611151975.XA Pending CN106520631A (en) | 2016-12-14 | 2016-12-14 | Complex microbial inoculant and application of composite bactericide to sewage treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106520631A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823141A (en) * | 2018-06-11 | 2018-11-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of oil smoke rubbish microbiological garbage treatment agent |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN113213642A (en) * | 2021-04-01 | 2021-08-06 | 浙江理工大学 | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101279796A (en) * | 2007-04-06 | 2008-10-08 | 阮承迈 | Domestic sewage process method |
| CN101942474A (en) * | 2010-06-11 | 2011-01-12 | 湖北大学 | Method for preparing whole-cell lipase preparation for treatment of oily sewage |
| CN105219687A (en) * | 2015-09-22 | 2016-01-06 | 宁波创蓝环境科技有限公司 | A kind of microbial preparation for the treatment of high grease sewage from catering business and application thereof |
-
2016
- 2016-12-14 CN CN201611151975.XA patent/CN106520631A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101279796A (en) * | 2007-04-06 | 2008-10-08 | 阮承迈 | Domestic sewage process method |
| CN101942474A (en) * | 2010-06-11 | 2011-01-12 | 湖北大学 | Method for preparing whole-cell lipase preparation for treatment of oily sewage |
| CN105219687A (en) * | 2015-09-22 | 2016-01-06 | 宁波创蓝环境科技有限公司 | A kind of microbial preparation for the treatment of high grease sewage from catering business and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吴兰等: "解脂耶氏酵母菌处理含油废水的研究", 《环境科学研究》 * |
| 胡南等: "沼泽红假单胞菌的分离•鉴定及生物净化作用研究", 《安徽农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823141A (en) * | 2018-06-11 | 2018-11-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of oil smoke rubbish microbiological garbage treatment agent |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN113213642A (en) * | 2021-04-01 | 2021-08-06 | 浙江理工大学 | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105621626B (en) | A kind of high-concentration chemical industry sewage composite bacteria agent and its application | |
| JP5027649B2 (en) | Female hormone substance-degrading microorganism and use thereof | |
| CN102586160B (en) | Stenotrophomonas maltophilia DS4 | |
| CN106520631A (en) | Complex microbial inoculant and application of composite bactericide to sewage treatment | |
| CN102676424B (en) | Obligate halophile for processing high-salt oily waste liquor in petroleum industry | |
| CN106635871A (en) | Complex bacteria and application thereof | |
| CN105087440B (en) | Pseudomonas mendocina NX-1 and its application in n-hexane degradation | |
| CN108823122A (en) | One plant of 2,4 dichloro benzene Phenol degrading bacteria and biodegrading process | |
| CN111440747B (en) | Compound microbial agent for sewage treatment, microecological preparation and application | |
| CN109706096A (en) | One plant of brevibacterium frigoritolerans and its application with denitrogenation and efficient flocculating ability | |
| CN109251880A (en) | A kind of Bacillus cereus and its application in improvement water systems'phosphorus pollution | |
| CN103255060A (en) | A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial | |
| CN102583782B (en) | Method for degrading phenol in waste water in coal chemical industry | |
| Narendran et al. | Bioremoval of toxic substances in synthetic wastewater using Trichoderma pubescens (NPK2), isolated from mangrove soil | |
| CN107794233A (en) | A kind of processing method of composite bacteria preparation and preparation method thereof and well-drilling waste water | |
| Rogers | Experimental evaluation of mycoremediation of Escherichia coli bacteria in solution using Pleurotus ostreatus | |
| CN111004749B (en) | Salt-tolerant bacillus lentus GBW-HB1902 and application thereof | |
| Liu et al. | Effect of inoculation on the biodegradation of butterfat-detergent mixtures in fixed-film sand columns | |
| CN103451127B (en) | Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof | |
| CN106834165A (en) | Secondary coccus, cell fraction of degradable penicillin and combinations thereof | |
| CN102220245B (en) | Aspergillus nomius SGFA1 and use of aspergillus nomius SGFA1 in degradation of formaldehyde | |
| CN105002114A (en) | Method for simply and rapidly separating and screening aerobic grease decomposing bacillus | |
| JP4577352B2 (en) | Novel clostridium species KD13 and purification method using the same | |
| CN106085923B (en) | A kind of preparation method and application of bacillus amyloliquefaciens and its biological flocculant | |
| CN110093278A (en) | A kind of extracting method and its application of potent algistatic activity substance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170322 |