CN102583782B - Method for degrading phenol in waste water in coal chemical industry - Google Patents
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Abstract
The invention relates to a method for degrading phenol in waste water in coal chemical industry, in particular to a method for degrading phenol in waste water in coal chemical industry by utilizing domesticated bacillus subtilis, which is characterized by including the following steps: a. preparing seed; b. domesticating degradation bacteria; c. separating single bacterial colony; d. culturing degradation phenol seed in amplifying mode; e. selecting phenol degradation bacteria; and f. introducing the phenol degradation bacteria selected in the step e. and receiving amplifying culture into the waste water in the coal chemical industry and culturing the phenol degradation bacteria for 24 to 72h under the temperature of 20-40 DEG C with the stirring speed as 100-130 r/min to degrade phenol in the waste water in the coal chemical industry. Compared with the prior art, the method can completely degrade phenol in waste water produced in coal preparation processes of carbinol, dimethyl ether and olefin, simultaneously has no secondary pollution to the environment and is unique in process.
Description
Technical field
The present invention relates to a kind of method of the phenol in waste water in coal chemical industry of degrading, especially relate to a kind of method of utilizing the subtilis degraded phenol in waste water in coal chemical industry after the domestication.
Background technology
The whole world to substitute industrial chemicals and for the urgent all the more background of the demand of the energy under, the Coal Chemical Industry industry of China becomes the important component part of energy structure in China with its leading industrialization progress, and country has also given increasing attention for coal chemical industry enterprises.At present, methyl alcohol, dme, propylene etc. are chemical main in the Chemical Industry chain.Yet, produce in row's the waste water in processes such as coal methyl alcohol processed, coal derived DME, olefin hydrocarbon making by coals, because containing the existence of phenol, effect is disposed in the processing that has greatly affected coal chemical industrial waste water.
Phenolic compound is a kind of prototype matter poisonous substance, and biont is had toxic action.Its contact by skin and mucous membrane sucks or direct oral cavity immerses in the organism, after protein in the magma in the cell contacts, form insoluble protein and cell is lost activity, especially neural system is had larger avidity, make neural system generation pathology.The common method of Phenol-Containing Wastewater Treatment mainly contains Physical, chemical method and biological process both at home and abroad at present.When the phenol content in the waste water during at 5~500mg/L, be suitable for processing with biological process.Studies show that yeast, root nodule bacterium, Acinetobacter calcoaceticus, pseudomonas, Alcaligenes eutrophus and denitrifying bacteria have certain degradation effect to phenol in wastewater.Phenol in the subtilis degradation treatment coal chemical industrial waste water after present method utilization domestication, have economy, efficient advantage, the more important thing is that subtilis is non-pathogenic bacteria, so can reach effect innoxious, non-secondary pollution degraded phenol in waste water in coal chemical industry.
Also there is not at present a kind of method of utilizing the subtilis degraded phenol in waste water in coal chemical industry after the domestication.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of method that drops into method low, simple to operate and can not cause the degraded phenol in waste water in coal chemical industry of secondary pollution to environment is provided.
The present invention realizes in the following way:
A kind of method of the phenol in waste water in coal chemical industry of degrading is characterized in that: the method comprises the steps:
A. seed preparation: picking one articulating enters in the enrichment medium from subtilis preservation pipe, after the activated and enlarged culturing of bacterial strain, be that 1 ~ 5% inoculum size is inoculated in the enrichment medium by mass percentage, aerobic cultivation 24 ~ 72h under temperature is 20 ~ 40 ℃ can be used for the degradation bacteria of taming;
B. the domestication of degradation bacteria: get the basis that by mass percentage 1 ~ 5% inoculum size access of seed that above-mentioned steps a makes contains 100mg/L phenol and select in the substratum, it is 20 ~ 40 ℃ in temperature, stir speed (S.S.) is under 100 ~ 130r/min behind aerobic cultivation 24 ~ 72h, get after the cultivation nutrient solution by mass percentage 1 ~ 5% inoculum size be inoculated in the basis that contains 200mg/L phenol and select in the substratum, cultivate 24 ~ 72h under the condition same as described above, by that analogy, namely get after previous step is cultivated nutrient solution by mass percentage 1 ~ 5% inoculum size be inoculated in phenol content and select in the substratum than the basis of the high 100mg/L of previous step, cultivate 24 ~ 72h under the condition same as described above, the nutrient solution of getting after the previous step inoculation culture for the last time, 1 ~ 5% inoculum size is inoculated in the basis selection substratum that contains 1000mg/L phenol by mass percentage, cultivates 24 ~ 72h under the condition same as described above;
C. the separation of single bacterium colony: the nutrient solution of selecting above-mentioned steps b turbidity maximum, coat the basis that contains 300mg/L phenol after the dilution and select the solid medium agar plate, under being 20 ~ 40 ℃, temperature cultivates 24 ~ 72h, the single bacterium colony that grows obtains the different single bacterium colony bacterial strain of form flat board line purifying 2 times;
D. degradation of phenol seed enlarged culturing: get the different shape list bacterium colony bacterial strain that above-mentioned steps c makes, be linked into respectively in the enrichment medium that contains 300mg/L phenol, be 20 ~ 40 ℃ in temperature, stir speed (S.S.) is aerobic cultivation 24 ~ 72h under 100 ~ 130r/min, can be used for the seed of degradation of phenol;
E. the selection of Phenol-degrading Bacteria Strains: the seed of the degradation of phenol that above-mentioned steps d is made, by mass percentage 1 ~ 5% inoculum size access respectively add 0,100,200 ..., 900, in the coal chemical industrial waste water of 1000mg/L phenol, it is 20 ~ 40 ℃ in temperature, under stir speed (S.S.) 100 ~ 130r/min behind aerobic cultivation 24 ~ 72h Pyrogentisinic Acid's concentration measure, selecting and making content is the bacterial strain that phenol in 0~200mg/L coal chemical industrial waste water can be degradable;
F. the Phenol-degrading Bacteria Strains of above-mentioned steps e being selected, the access enrichment medium in by mass percentage 1 ~ 5% the inoculum size access coal chemical industrial waste water, is 20 ~ 40 ℃ in temperature after enlarged culturing, aerobic cultivation 24 ~ 72h under stir speed (S.S.) 100 ~ 130r/min, i.e. phenol in the degradable coal chemical industrial waste water;
Described enrichment medium, substratum is selected on the basis, and the basis selects the solid medium preparation method to be respectively:
1) preparation of enrichment medium: the raw material of following weight mixed to make enrichment medium: peptone 5.0g, beef diffusion juice 3.0g, NaCl 5.0g, MnSO
4H
2O 0.01g, water 1000mL;
2) preparation of substratum is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, water 1000mL;
3) preparation of solid medium is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, agar 20g, water 1000mL;
Described step b contain 100,200 ..., 900, the basis of 1000mg/L phenol select substratum be by the basis select substratum 1000mL respectively with 100,200 ..., 900,1000mg phenol mixes;
It is to select substratum 1000mL, phenol 300mg and agar 20g to mix by the basis that solid medium is selected on the basis that contains 300mg/L phenol of described step c;
The enrichment medium that contains 300mg/L phenol of described steps d is to be mixed by enrichment medium 1000mL and phenol 300mg;
Described step e contain 0,100,200 ..., 900, the preparation of the coal chemical industrial waste water of 1000mg/L phenol be according to add respectively 0,100,200 in every 1000mL coal chemical industrial waste water ..., 900,1000mg phenol mixes;
Described coal chemical industrial waste water is the waste water that is derived from coal methyl alcohol processed, coal derived DME, the generation of olefin hydrocarbon making by coal process, and its phenol content is 20 ~ 160mg/L.
The present invention has following effect:
1) degradation technique is unique: the method for a kind of phenol in waste water in coal chemical industry of degrading provided by the invention, comprise seed preparation, degradation bacteria domestication, degradation bacteria selection, then with the Phenol-degrading Bacteria Strains of selecting, after enlarged culturing, access by a certain percentage in the coal chemical industrial waste water, be 20 ~ 40 ℃ in temperature, aerobic cultivation 24 ~ 72h under stir speed (S.S.) 100 ~ 130r/min, i.e. phenol in the degradable coal chemical industrial waste water.
2) degradable phenol in the coal chemical industrial waste water: because the waste water of coal methyl alcohol processed, coal derived DME, the generation of olefin hydrocarbon making by coal process, its phenol content is between 20 ~ 160mg/L.So, utilize method provided by the invention, can degradable coal methyl alcohol processed, coal derived DME, olefin hydrocarbon making by coal process produce the phenol in the waste water.
3) environmental sound: subtilis used in the present invention is non-pathogenic bacteria, is different from other microorganism, and the phenol in the coal chemical industrial waste water finally can be converted into carbonic acid gas and water, can not cause secondary pollution to environment.
Embodiment
Embodiment 1:
A kind of method of the phenol in waste water in coal chemical industry of degrading, the method comprises the steps:
A. seed preparation: preserve picking one ring the pipe from subtilis, in the access enrichment medium, after the activated and enlarged culturing of bacterial strain, be that 5% inoculum size is inoculated in the enrichment medium by mass percentage, aerobic cultivation 24h under temperature is 40 ℃ can be used for the degradation bacteria of taming;
B. the domestication of degradation bacteria: get the basis that by mass percentage 5% inoculum size access of seed that above-mentioned steps a makes contains 100mg/L phenol and select in the substratum, it is 40 ℃ in temperature, stir speed (S.S.) is under the 130r/min behind the aerobic cultivation 24h, get after the cultivation nutrient solution by mass percentage 5% inoculum size be inoculated in the basis that contains 200mg/L phenol and select in the substratum, cultivate 24h under the condition same as described above, by that analogy, namely get after previous step is cultivated nutrient solution by mass percentage 5% inoculum size be inoculated in phenol content and select in the substratum than the basis of the high 100mg/L of previous step, cultivate 24h under the condition same as described above, the nutrient solution of getting after the previous step inoculation culture for the last time, 5% inoculum size is inoculated in the basis selection substratum that contains 1000mg/L phenol by mass percentage, cultivates 24h under the condition same as described above;
C. the separation of single bacterium colony: the nutrient solution of selecting above-mentioned steps b turbidity maximum, coat the basis that contains 300mg/L phenol after the dilution and select the solid medium agar plate, under being 40 ℃, temperature cultivates 24h, the single bacterium colony that grows obtains the different single bacterium colony bacterial strain of form flat board line purifying 2 times;
D. degradation of phenol seed enlarged culturing: get the different shape list bacterium colony bacterial strain that above-mentioned steps c makes, be linked into respectively the enrichment medium that contains 300mg/L phenol, be 40 ℃ in temperature, stir speed (S.S.) is aerobic cultivation 24h under the 130r/min, can be used for the seed of degradation of phenol;
E. the selection of Phenol-degrading Bacteria Strains: the seed of the degradation of phenol that above-mentioned steps d is made, by mass percentage 5% inoculum size access respectively add 0,100,200 ..., 900, in the coal chemical industrial waste water of 1000mg/L phenol, it is 40 ℃ in temperature, under the stir speed (S.S.) 130r/min behind the aerobic cultivation 24h Pyrogentisinic Acid's concentration measure, selecting and making content is the bacterial strain that phenol in 0~200mg/L coal chemical industrial waste water can be degradable;
F. the Phenol-degrading Bacteria Strains of above-mentioned steps e being selected, the access enrichment medium in by mass percentage 5% the inoculum size access coal chemical industrial waste water, is 40 ℃ in temperature after enlarged culturing, aerobic cultivation 24h, i.e. phenol in the degradable coal chemical industrial waste water under the stir speed (S.S.) 130r/min.
Coal chemical industrial waste water is taken from the waste water that coal methanol process processed is produced, and after processing with aforesaid method, through the amino antipyrine spectrophotometry of 4-, does not detect the existence of phenol in the waste water.
Embodiment 2:
A. seed preparation: picking one articulating enters in the enrichment medium from subtilis preservation pipe, after the activated and enlarged culturing of bacterial strain, being that 3% inoculum size is inoculated in enrichment medium by mass percentage, is 30 ℃ of lower aerobic cultivations 48h in temperature, can be used for the degradation bacteria of taming;
B. the domestication of degradation bacteria: get the basis that by mass percentage 3% inoculum size access of seed that above-mentioned steps a makes contains 100mg/L phenol and select in the substratum, it is 30 ℃ in temperature, stir speed (S.S.) is under the 115r/min behind the aerobic cultivation 48h, get after the cultivation nutrient solution by mass percentage 3% inoculum size be inoculated in the basis that contains 200mg/L phenol and select in the substratum, cultivate 48h under the condition same as described above, by that analogy, namely get after previous step is cultivated nutrient solution by mass percentage 3% inoculum size be inoculated in phenol content and select to cultivate 48h under the condition same as described above in the substratum than the basis of the high 100mg/L of previous step.Be the nutrient solution of getting after the previous step inoculation culture for the last time, 3% inoculum size is inoculated in the basis that contains 1000mg/L phenol and selects to cultivate 48h under the condition same as described above in the substratum by mass percentage;
C. the separation of single bacterium colony: the nutrient solution of selecting above-mentioned steps b turbidity maximum, coat the basis that contains 300mg/L phenol after the dilution and select the solid medium agar plate, under being 30 ℃, temperature cultivates 48h, the single bacterium colony that grows obtains the different single bacterium colony bacterial strain of form flat board line purifying 2 times;
D. degradation of phenol seed enlarged culturing: get the different shape list bacterium colony bacterial strain that above-mentioned steps c makes, be linked into respectively the enrichment medium that contains 300mg/L phenol, be 30 ℃ in temperature, stir speed (S.S.) is aerobic cultivation 48h under the 115r/min, can be used for the seed of degradation of phenol;
E. the selection of Phenol-degrading Bacteria Strains: the seed of the degradation of phenol that above-mentioned steps d is made, by mass percentage 3% inoculum size access respectively add 0,100,200 ..., 900, in the coal chemical industrial waste water of 1000mg/L phenol, it is 30 ℃ in temperature, under the stir speed (S.S.) 115r/min behind the aerobic cultivation 48h Pyrogentisinic Acid's concentration measure, selecting and making content is the bacterial strain that phenol in 0~200mg/L coal chemical industrial waste water can be degradable;
F. the Phenol-degrading Bacteria Strains of above-mentioned steps e being selected, the access enrichment medium in by mass percentage 3% the inoculum size access coal chemical industrial waste water, is 30 ℃ in temperature after enlarged culturing, aerobic cultivation 48h, i.e. phenol in the degradable coal chemical industrial waste water under the stir speed (S.S.) 115r/min.
Coal chemical industrial waste water is taken from the waste water that the coal derived DME process is produced, and after processing with aforesaid method, through the amino antipyrine spectrophotometry of 4-, does not detect the existence of phenol in the waste water.
Embodiment 3:
A. seed preparation: picking one articulating enters in the enrichment medium from subtilis preservation pipe, after the activated and enlarged culturing of bacterial strain, being that 1% inoculum size is inoculated in enrichment medium by mass percentage, is 20 ℃ of lower aerobic cultivations 72h in temperature, can be used for the degradation bacteria of taming;
B. the domestication of degradation bacteria: get the basis that by mass percentage 1% inoculum size access of seed that above-mentioned steps a makes contains 100mg/L phenol and select in the substratum, it is 20 ℃ in temperature, stir speed (S.S.) is under the 100r/min behind the aerobic cultivation 72h, get after the cultivation nutrient solution by mass percentage 1% inoculum size be inoculated in the basis that contains 200mg/L phenol and select in the substratum, cultivate 72h under the condition same as described above, by that analogy, namely get after previous step is cultivated nutrient solution by mass percentage 1% inoculum size be inoculated in phenol content and select to cultivate 72h under the condition same as described above in the substratum than the basis of the high 100mg/L of previous step.Be the nutrient solution of getting after the previous step inoculation culture for the last time, 1% inoculum size is inoculated in the basis that contains 1000mg/L phenol and selects to cultivate 72h under the condition same as described above in the substratum by mass percentage;
C. the separation of single bacterium colony: the nutrient solution of selecting above-mentioned steps b turbidity maximum, coat the basis that contains 300mg/L phenol after the dilution and select the solid medium agar plate, under being 20 ℃, temperature cultivates 72h, the single bacterium colony that grows obtains the different single bacterium colony bacterial strain of form flat board line purifying 2 times;
D. degradation of phenol seed enlarged culturing: get the different shape list bacterium colony bacterial strain that above-mentioned steps c makes, be linked into respectively the enrichment medium that contains 300mg/L phenol, be 20 ℃ in temperature, stir speed (S.S.) is aerobic cultivation 72h under the 100r/min, can be used for the seed of degradation of phenol;
E. the selection of Phenol-degrading Bacteria Strains: the seed of the degradation of phenol that above-mentioned steps d is made, by mass percentage 1% inoculum size access respectively add 0,100,200 ..., 900, in the coal chemical industrial waste water of 1000mg/L phenol, it is 20 ℃ in temperature, under the stir speed (S.S.) 100r/min behind the aerobic cultivation 72h Pyrogentisinic Acid's concentration measure, selecting and making content is the bacterial strain that phenol in 0~200mg/L coal chemical industrial waste water can be degradable;
F. the Phenol-degrading Bacteria Strains of above-mentioned steps e being selected, the access enrichment medium in by mass percentage 1% the inoculum size access coal chemical industrial waste water, is 20 ℃ in temperature after enlarged culturing, aerobic cultivation 72h, i.e. phenol in the degradable coal chemical industrial waste water under the stir speed (S.S.) 100r/min.
Coal chemical industrial waste water is taken from the waste water that the olefin hydrocarbon making by coal process is produced, and after processing with aforesaid method, through the amino antipyrine spectrophotometry of 4-, does not detect the existence of phenol in the waste water.
Above-mentioned enrichment medium, substratum is selected on the basis, solid medium is selected on the basis, contain 100,200 ..., 900, the basis of 1000mg/L phenol selects substratum, solid medium is selected on the basis that contains 300mg/L phenol, the enrichment medium that contains 300mg/L phenol, contain 0,100,200 ..., 900, the coal chemical industrial waste water preparation method of 1000mg/L phenol is respectively:
1) preparation of enrichment medium: the raw material of following weight mixed to make enrichment medium: peptone 5.0g, beef diffusion juice 3.0g, NaCl 5.0g, MnSO
4H
2O 0.01g, water 1000mL;
2) preparation of substratum is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, water 1000mL;
3) preparation of solid medium is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, agar 20g, water 1000mL;
Above-mentionedly contain 100,200 ..., 900, the basis of 1000mg/L phenol selects the substratum preparation: the basis of above-mentioned steps b preparation select substratum 1000mL respectively with 100,200 ..., 900,1000mg phenol mixes;
The solid medium preparation is selected on the basis of the above-mentioned 300mg/L of containing phenol: the basis of above-mentioned steps b preparation selects substratum 1000mL and phenol 300mg, agar 20g to mix;
The enrichment medium preparation of the above-mentioned 300mg/L of containing phenol: enrichment medium 1000mL and the phenol 300mg of above-mentioned steps a preparation mix;
Above-mentionedly contain 0,100,200 ..., 900, the preparation of the coal chemical industrial waste water of 1000mg/L phenol be according to add respectively 0,100,200 in every 1000mL coal chemical industrial waste water ..., 900,1000mg phenol mixes.
The described bacterial strain of present method is subtilis
Bacillus Subtilis(CGMCC 1.2172) are preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center.
The amino antipyrine spectrophotometry of 4-was adopted in the phenol in waste water in coal chemical industry concentration determination before and after subtilis after the described utilization domestication of present method was processed, concrete steps are: get and process front and back waste water 5mL, place the 10mL centrifuge tube, at 10000r/min, 4 ℃ of lower centrifugal 5min.Then take the amino antipyrine of 4-as indicator, in the variation of UV, visible light spectrophotometer mensuration absorbance, according to phenol Standard concentration-absorbancy curve, obtain the concentration of phenol in wastewater.
Claims (7)
1. the method for the phenol in waste water in coal chemical industry of degrading, it is characterized in that: the method comprises the steps:
A. seed preparation: picking one articulating enters in the enrichment medium from subtilis preservation pipe, after the activated and enlarged culturing of bacterial strain, be that 1 ~ 5% inoculum size is inoculated in the enrichment medium by mass percentage, aerobic cultivation 24 ~ 72h under temperature is 20 ~ 40 ℃ can be used for the degradation bacteria of taming;
B. the domestication of degradation bacteria: get the basis that by mass percentage 1 ~ 5% inoculum size access of seed that above-mentioned steps a makes contains 100mg/L phenol and select in the substratum, it is 20 ~ 40 ℃ in temperature, stir speed (S.S.) is under 100 ~ 130r/min behind aerobic cultivation 24 ~ 72h, get after the cultivation nutrient solution by mass percentage 1 ~ 5% inoculum size be inoculated in the basis that contains 200mg/L phenol and select in the substratum, cultivate 24 ~ 72h under the condition same as described above, by that analogy, namely get after previous step is cultivated nutrient solution by mass percentage 1 ~ 5% inoculum size be inoculated in phenol content and select in the substratum than the basis of the high 100mg/L of previous step, cultivate 24 ~ 72h under the condition same as described above, the nutrient solution of getting after the previous step inoculation culture for the last time, 1 ~ 5% inoculum size is inoculated in the basis selection substratum that contains 1000mg/L phenol by mass percentage, cultivates 24 ~ 72h under the condition same as described above;
C. the separation of single bacterium colony: the nutrient solution of selecting above-mentioned steps b turbidity maximum, coat the basis that contains 300mg/L phenol after the dilution and select the solid medium agar plate, under being 20 ~ 40 ℃, temperature cultivates 24 ~ 72h, the single bacterium colony that grows obtains the different single bacterium colony bacterial strain of form flat board line purifying 2 times;
D. degradation of phenol seed enlarged culturing: get the different shape list bacterium colony bacterial strain that above-mentioned steps c makes, be linked into respectively in the enrichment medium that contains 300mg/L phenol, be 20 ~ 40 ℃ in temperature, stir speed (S.S.) is aerobic cultivation 24 ~ 72h under 100 ~ 130r/min, can be used for the seed of degradation of phenol;
E. the selection of Phenol-degrading Bacteria Strains: the seed of the degradation of phenol that above-mentioned steps d is made, by mass percentage 1 ~ 5% inoculum size access respectively add 0,100,200 ..., 900, in the coal chemical industrial waste water of 1000mg/L phenol, it is 20 ~ 40 ℃ in temperature, under stir speed (S.S.) 100 ~ 130r/min behind aerobic cultivation 24 ~ 72h Pyrogentisinic Acid's concentration measure, selecting and making content is the bacterial strain that phenol in 0~200mg/L coal chemical industrial waste water can be degradable;
F. the Phenol-degrading Bacteria Strains of above-mentioned steps e being selected, the access enrichment medium in by mass percentage 1 ~ 5% the inoculum size access coal chemical industrial waste water, is 20 ~ 40 ℃ in temperature after enlarged culturing, aerobic cultivation 24 ~ 72h under stir speed (S.S.) 100 ~ 130r/min, i.e. phenol in the degradable coal chemical industrial waste water.
2. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1 is characterized in that: described enrichment medium, and substratum is selected on the basis, and the basis selects the solid medium preparation method to be respectively:
1) preparation of enrichment medium: the raw material of following weight mixed to make enrichment medium: peptone 5.0g, beef diffusion juice 3.0g, NaCl 5.0g, MnSO
4H
2O 0.01g, water 1000mL;
2) preparation of substratum is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, water 1000mL;
3) preparation of solid medium is selected on the basis: the raw material of following weight is mixed can make the basis and select substratum: K
2HPO
40.5g, KH
2PO
40.5g, NaCl 0.2g, NH
4NO
31.0g, MgSO
40.2g, CaCl
20.2g, MnSO
4H
2O 0.01g, agar 20g, water 1000mL.
3. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1 is characterized in that: described step b contain 100,200 ..., 900, the basis of 1000mg/L phenol select substratum be by the basis select substratum 1000mL respectively with 100,200 ..., 900,1000mg phenol mixes.
4. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1 is characterized in that: it is to select substratum 1000mL, phenol 300mg and agar 20g to mix by the basis that solid medium is selected on the basis that contains 300mg/L phenol of described step c.
5. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1, it is characterized in that: the enrichment medium that contains 300mg/L phenol of described steps d is to be mixed by enrichment medium 1000mL and phenol 300mg.
6. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1 is characterized in that: described step e contain 0,100,200 ..., 900, the preparation of the coal chemical industrial waste water of 1000mg/L phenol be according to add respectively 0,100,200 in every 1000mL coal chemical industrial waste water ..., 900,1000mg phenol mixes.
7. the method for a kind of phenol in waste water in coal chemical industry of degrading as claimed in claim 1 is characterized in that: described coal chemical industrial waste water is to be derived from the waste water that coal methyl alcohol processed, coal derived DME, olefin hydrocarbon making by coal process produce, and its phenol content is 20 ~ 160mg/L.
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CN103060420A (en) * | 2012-12-25 | 2013-04-24 | 天津大学 | Interaction of polyphenol pollutants in the process of biodegradation and a research method thereof |
CN106673204A (en) * | 2016-06-06 | 2017-05-17 | 武汉虹睿生物科技开发有限公司 | Application of bacillus mojavensis KJS-3 to decomposition of phenol |
CN107792940A (en) * | 2016-08-30 | 2018-03-13 | 江苏吉华化工有限公司 | A kind of method of phenol in degraded wastewater from chemical industry |
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Citations (2)
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CN1059165A (en) * | 1991-04-01 | 1992-03-04 | 陈绍维 | Secondary reformer gas station phenol water treatment technique and device |
CN2873784Y (en) * | 2006-01-13 | 2007-02-28 | 淄博东能煤气工程技术开发有限公司 | Industrial gas phenolic liquid evaporator |
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CN1059165A (en) * | 1991-04-01 | 1992-03-04 | 陈绍维 | Secondary reformer gas station phenol water treatment technique and device |
CN2873784Y (en) * | 2006-01-13 | 2007-02-28 | 淄博东能煤气工程技术开发有限公司 | Industrial gas phenolic liquid evaporator |
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