CN106673204A - Application of bacillus mojavensis KJS-3 to decomposition of phenol - Google Patents
Application of bacillus mojavensis KJS-3 to decomposition of phenol Download PDFInfo
- Publication number
- CN106673204A CN106673204A CN201610415649.9A CN201610415649A CN106673204A CN 106673204 A CN106673204 A CN 106673204A CN 201610415649 A CN201610415649 A CN 201610415649A CN 106673204 A CN106673204 A CN 106673204A
- Authority
- CN
- China
- Prior art keywords
- phenol
- kjs
- culture medium
- hawei
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
- C02F2101/345—Phenols
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to application of bacillus mojavensis KJS-3 to decomposition of phenol, and belongs to the field of wastewater treatment. Removal of phenolic compounds is a key issue in wastewater treatment. The invention mainly focuses on identification of a strain for efficiently degrading the phenol, namely the bacillus mojavensis KJS-3 (with the strain collection number of KCCM10961P). Experiments prove that the strain is a potential environment-friendly strain having the capability of degrading the phenol in wastewater.
Description
Technical field
The present invention relates to Mo Hawei bacillus cereuss KJS-3 is used to decompose the purposes of phenol, belong to field of waste water treatment.
Background technology
Do not only have organic substance in various industrial wastewaters now and also include the refractory organicses compound for decomposing difficulty.These
In refractory organicses compound, toxicity is not only produced to human body but also is become because of natural decomposition difficulty and cause the main of environmental pollution
One of material, so as to cause the appearance of its Environmental Residues problem.Especially as the fast development of chemical industry, make in various techniques
Aromatic compound has benzene ring structure, and it not only can produce toxicity to most human body, because decomposing tired in nature
Difficulty will also become one of main cause material for causing environmental pollution, and its usage amount is also in being continuously increased.Phenol by
EPA regards as the chemicals of major pollutant, and below its concentration 200mg/l growth of microorganism is may interfere with, and is useless
Environmental Pollution material difficult to deal with water.
Emerged in an endless stream using the research of microbial treatments waste water both at home and abroad.Phenol based compound is to pass through hydroxyl by microorganism
Change forms catechol mesostate, then divides aromatic rings by the effect of enzyme, and one kind is resolved into Orthopathways
Succinic acid and S-acetyl-coenzyme-A, another kind be with meta-cleavage pathway resolve into acetaldehyde and acetone acid (Aldrich, T.L., Frantz,
B., Gill, J.F., Kilbane, J.J.and Chakrabarty, A.M.1987.Cloning and complete
Nucleotide sequence determination of the cat B gene encoding cis, cis-muconate
Lactonizing enzyme.Gene 52,185-195.).
But the efficiency of existing microorganism is very low in this metabolic process, the various garbages contained in waste water are difficult rapidly
Catabolism fall.And these intermediate materials are dissolved in waste water, the activity of related microorganism can be reduced so as to make
Further decline into waste water treatment efficiency, and because the toxic component of those chemical substances is discharged in nature, can also make
Into secondary environmental pollution.
Oxybenzene compound is the material for using in a large number in the industrial production, and related chemistry wastewater treatment is asking for a sternness
Topic.So oxybenzene compound decomposes the screening of microorganism, and it is particularly significant in various waste water treatment applications, in order to be directed to this kind ofization
The selected of the screening and separation of the microorganism of compound, separation source and metabolite becomes particularly significant.
The content of the invention
The technical problem to be solved is to provide Mo Hawei bacillus cereuss KJS-3 for decomposing the purposes of phenol.
The capacity of decomposition of Mo Hawei bacillus cereuss KJS-3 Pyrogentisinic Acids of the present invention has improvement, can be used for containing phenol
Various sewage and waste waters process.
The present invention is filtered out with outstanding clear for the wastewater treatment of the various industrial undertakings such as chemical plant and paper mill
The microbial strains Mo Hawei bacillus cereuss KJS-3 (Bacillus mojavensis KJS-3) of removing solid capacity.Mo Hawei spore bars
Bacterium (bacillus mojavensis) KJS-3 was preserved in Korean Culture Center, deposit number on 22nd in August in 2008
For KCCM 10961P.The growth conditionss of the bacterium are：20~50 DEG C, pH=6~7.5.
The microorganism that the present invention is used, it is under the conditions of aerobic, and flourish is all right and Pyrogentisinic Acid's compound
Capacity of decomposition is improved.This bacterial strain has outstanding phenol capacity of decomposition, is confirmed in the present invention.The opposing party of the present invention
Face provides a kind of method of the wastewater treatment containing oxybenzene compound.
Description of the drawings
The absorbance result that Fig. 1 is determined in 600nm wavelength：Sample concentration is respectively phenol 500ppm, 250ppm,
Result (remarks-the ppm of the Mo Hawei bacillus cereuss KJS-3 bacterial strains grown up during 50ppm：PPM, 1ppm=1mg/L).
Fig. 2 contains sample phenol 500ppm, and the culture medium of 250ppm, 50ppm is cultivated at 40 DEG C and makes Mo Hawei spore bars
Bacterium KJS-3 grows, and Mo Hawei bacillus cereuss KJS-3 bacterial strains grow feelings in culture dish after this culture fluid each concentration dilution
Condition.
Fig. 3 contains sample phenol 1000ppm, and the culture medium of 2000ppm, 3000ppm is cultivated at 40 DEG C and makes Mo Hawei buds
Spore bacillus KJS-3 grows, and Mo Hawei bacillus cereuss KJS-3 bacterial strains grow in culture dish after this culture fluid each concentration dilution
Situation.
Fig. 4 contains sample phenol 1000ppm, and the culture medium of 2000ppm, 3000ppm is cultivated at 25 DEG C and makes Mo Hawei buds
Spore bacillus KJS-3 grows, and Mo Hawei bacillus cereuss KJS-3 bacterial strains grow in culture dish after this culture fluid each concentration dilution
Situation, but sample phenol 3,000ppm bacterial strains when diluting 100,000 times cannot confirm into long status.
Specific embodiment
It is specifically addressed explanation to the present invention below, the present invention is not limited solely to cited exemplary application.
The separation of the bacterial strain of embodiment 1
The stickum part that mycete cannot grow in food refuse gathers out, in moving on to laboratory, in tryptone soy
Cultivate in shaking table under conditions of 37 DEG C, 16 hours in agar culture medium (TSA). the bacterium colony of growth is selected here, each
It is inoculated in trypticase soy broth (TSB) and is taken care of, the bacterial strain of growth is taken care of respectively in 45 DEG C and 20 DEG C
Cultivated, then taken care of cultivating among these after the bacterial strain that grows out is selected again. by the bacterial strain of these selection keepings 4
Take care of 30 days in DEG C, then by this again under the conditions of 37 DEG C, culture in tryptone soya broth liquid culture medium (TSA). 10
Cultivate 7 days and have wherein 2 bacterial strains of endospore (Endospore) formation to select under the conditions of 37 DEG C in the culture fluid planted
Come, be respectively No. 3 and No. 4 bacterial strains by Strain Designation.
These bacterial strains entrust respectively Korean Culture Center to carry out obtaining Mo Hawei spore bars after hereditary material analysis
Bacterium KJS-3 (Bacillus mojavensis KJS-3) and Mo Hawei bacillus cereuss KJS-4 (Bacillus mojavensis
KJS-4) bacterial strain, and carried out bacterial strain strain registration.Mo Hawei bacillus cereuss KJS-3 (Bacillus mojavensis KJS-
3) in Korean Culture Center Bacillus mojavensis KJS-3 (deposit numbers：KCCM10961P) preservation is carried out.
Embodiment 2 has the confirmation of phenol capacity of decomposition
Above-mentioned 2 kinds of microorganism Mo Hawei bacillus cereuss KJS-3 (Bacillus mojavensis KJS-3) and Mo Hawei
Bacillus cereuss KJS-4 (Bacillus mojavensis KJS-4) in 40 DEG C of temperature, tryptone soya broth culture medium (TSA)
Middle inoculated and cultured. entered using the Mo Hawei bacillus cereuss KJS-3 (Bacillus mojavensis KJS-3) cultivated in 40 DEG C
Capable this experiment.
1, Mo Hawei bacillus cereuss KJS-3 bacterial strains in 10ml TSB inoculation of mediums, 40 DEG C, culture more than 16 hours.
5ml is taken in culture fluid after cultivating 16 hours out, be inoculated with 100ml TSB, train under 40 DEG C of temperature conditionss
Support 90 minutes, mixing speed remains 100~200rpm.
2, the culture cultivated in above-mentioned steps takes 500 μ l (microlitre) in 10,000rpm, centrifugation point under conditions of 1 minute
From and remove the supernatant, reinject the normal saline (0.9%NaCl) of 500 μ l, minimum culture is inoculated in after fully mixing
In base.
3, according to the different time, in 0min, 30min, 60min, 2 hour, 3 hours, 4 hours, 5 hours, 6 hours, 7 is little
When and after 8 hours, mensuration absorbance (O.D) value, determines the growth curve of bacterial strain in UV, visible light spectrophotometer.
The composition of minimum culture medium is as follows：(1 liter, pH7.0) NH4Cl 1.0g, K2HPO44.35g, NaH2PO43.9g,
MgSO4.7H2O 0.48g CaCl20.03g, FeSO40.01g, MnCl20.01g, CoCl20.001g, Na2MnO4
0.001g。
3 minimum culture medium are made, respectively as 50ppm, 250ppm, 500ppm is made and tried the concentration with phenol
Test, absorbance is determined as follows under 600nm wavelength：
| 50ppm phenol | 250ppm phenol | 500ppm phenol | |
| 0min | 0.032 | 0.032 | 0.032 |
| 30min | 0.057 | 0.07 | 0.066 |
| 1hr | 0.074 | 0.076 | 0.094 |
| 2hrs | 0.114 | 0.130 | 0.161 |
| 3hrs | 0.150 | 0.181 | 0.193 |
| 4hrs | 0.312 | 0.331 | 0.317 |
| 5hrs | 0.519 | 0.524 | 0.539 |
| 6hrs | 0.721 | 0.687 | 0.688 |
| 7hrs | 0.754 | 0.704 | 0.735 |
| 8hrs | 0.731 | 0.705 | 0.691 |
Understand in from the above, Mo Hawei bacillus cereuss KJS-3 bacterial strains decompose utilization using phenol as carbon, and 7 is little
Shi Houcheng maximum growth states, with reference to Fig. 1.
Embodiment 3
The phenol content for decomposing bacterial strain for phenol is determined, and according to following order reaction experiment is carried out, and is entered in wavelength 650nm
Row absorbance measurement.
1) culture fluid is gone to refrigerator keeping standby by bacterial strain after culture 8 hours.
2) phenol is quantitative
(1) prepared by standard substance：250ppm- phenol, 25ppm- phenol, 12.5ppm- phenol.
(2) sample：500ppm- phenol, comprising a small amount of culture medium (40 DEG C, culture 8 hours)；
250ppm- phenol include a small amount of culture medium, 50ppm- phenol comprising a small amount of culture medium (40 DEG C, culture 8 hours).
(3) experimentation：Standard substance and each sample respectively take respectively 100ml, plus the 8wt%K of 6ml3Fe(CN)6, 6ml
0.1M FeCl3After (0.1M HCl make solvent), after reacting 5 minutes, mensuration absorbance value (OD) at wavelength 650nm.
As a result it is as shown in the table：
As can be known from the above table, the OD values in sample 500ppm are 1.304, and the phenol that there are about 17ppm is detected, and illustrates it
The phenol of 483ppm is decomposed in 500ppm, and the OD values in sample 250ppm are 1.108, and the phenol that there are about 14ppm is tested
Go out, the phenol for illustrating 236ppm in 250ppm is decomposed, finally the OD values in sample 50ppm are 1.084, and explanation there are about
The phenol of 13ppm is detected, and the phenol of 37ppm is decomposed, so, Mo Hawei bacillus cereuss KJS-3 bacterial strains are directed in the present invention
The characteristics of capacity of decomposition of oxybenzene compound is outstanding is proved.
Embodiment 4
The Mo Hawei bacillus cereuss KJS-3 bacterial strains cultivated in embodiment 2 carry out dilution stage by stage, confirm its bacterial strain shape
Condition.
With 102, 104, 105, 106Concentration is diluted, after the 0.5ml that respectively asks for, at tryptone soya broth culture medium (TSA)
On equably drawout cultivated, clump count is selected in the culture dish of a diameter of 90mm from 30 to less than 300, and with original
Make comparisons come the bacteria concentration in the culture medium cultivated.
Understand in Fig. 2, there are 103 bacterium colonies in the phenol culture medium containing 500ppm, extension rate is 105, originally cultivate
Belong to 2 × 10 in base7The bacterial strain of cfu/ml.
There are 37 bacterium colonies in the culture medium of the phenol containing 250ppm, extension rate is 105, it is 8 that this is original culture medium
×106The bacterial strain of cfu/ml concentration.There are 32 bacterium colonies in the culture medium of the phenol containing 25ppm, extension rate is 105, this is former
Carry out culture medium for 6 × 106The bacterial strain of cfu/ml concentration.
Mo Hawei bacillus cereuss KJS-3 bacterial strains are can be seen that till phenol content is 500ppm, grow from this result
In good condition, and to 50ppm, 250ppm, 500ppm is done if comparing, and bacterium number increases most in 500ppm, is at least training
In foster base, phenol is sole carbon source, and also decomposable asymmetric choice net is utilized and normal growth Mo Hawei bacillus cereuss KJS-3.
Embodiment 5
Mo Hawei bacillus cereuss KJS-3 bacterial strains in TSB culture medium, incubated overnight, containing phenol 6 minimum culture medium
Prepare inoculation, wherein 3 culture medium are cultivated under the conditions of 25 DEG C, another 3 culture medium are cultivated under the conditions of 40 DEG C, at this moment, each
The concentration containing phenol of temperature is dilution 10 after culture in 1,000ppm, 2,000ppm, 3,000ppm, 16 hours5Or 106Again simultaneously
Equably capable culture, culture medium TSA of a diameter of 90mm are driven in tiling in tryptone soya broth culture medium (TSA) to take 0.5ml
The bacterium number of middle growth is confirmed.
Understand in Fig. 3, the 1 of 1,000ppm phenol, in 000,000 times of diluted medium is contained under the conditions of 40 DEG C, bacterium number
Respectively 65,49, this and original culture medium 1.2 × 108Cfu/ml related bacterium number compares the level for belonging at a relatively high, but
In being 100,000 times of diluted medium containing 2,000ppm and 3,000ppm phenol, bacterium number is 5,13, although Ke Yifen
Solution utilizes phenol, but can be seen that growth inhibiting situation occur, so, Mo Hawei bacillus cereuss KJS-3 is 40 DEG C in temperature
When, can grow in 1, the 000ppm culture medium containing phenol.
Can be seen that under the conditions of 25 DEG C, in 100,000 times of diluted medium of the phenol for containing 1,000ppm in Fig. 4
Bacterium colony is 98, originally culture medium 2 × 106The bacterium number of cfu/ml concentration.But containing 2,000ppm phenol 100,000 times dilute
In releasing culture medium, detection bacterium number is 11,13, although can decompose using phenol but occur growth inhibited and show
As, and contain in 100,000 times of diluted medium of 3,000ppm phenol, bacterium number is not detected substantially, only 1~2 inspection
Go out, the growth inhibited phenomenon very strong to it occur.
So, Mo Hawei bacillus cereuss KJS-3 contains in 25 DEG C of temperature can also in the culture medium of 1,000ppm phenol
Growth, but with compared with 40 DEG C, the speed of growth has declined.
Claims (5)
1. Mo Hawei bacillus cereuss KJS-3 is used to decompose the purposes of phenol.
2. purposes according to claim 1, it is characterised in that the Mo Hawei bacillus cereuss KJS-3 is in Korean Culture
Collection carries out preservation, deposit number：KCCM10961P.
3. purposes according to claim 1 and 2, it is characterised in that the Mo Hawei bacillus cereuss KJS-3 contains for process
The purposes of the waste water of phenol.
4. purposes according to claim 3, it is characterised in that the concentration of described phenol in wastewater is 1000~
3000ppm。
5. purposes according to claim 3, it is characterised in that described wastewater temperature is 25~40 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610415649.9A CN106673204A (en) | 2016-06-06 | 2016-06-06 | Application of bacillus mojavensis KJS-3 to decomposition of phenol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610415649.9A CN106673204A (en) | 2016-06-06 | 2016-06-06 | Application of bacillus mojavensis KJS-3 to decomposition of phenol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106673204A true CN106673204A (en) | 2017-05-17 |
Family
ID=58839678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610415649.9A Pending CN106673204A (en) | 2016-06-06 | 2016-06-06 | Application of bacillus mojavensis KJS-3 to decomposition of phenol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106673204A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974459A (en) * | 2010-10-12 | 2011-02-16 | 黑龙江省科学院微生物研究所 | Microbes capable of degrading phenol and cyanogen in coking waste water and method for treating coking waste water by using same |
| CN102344899A (en) * | 2011-10-21 | 2012-02-08 | 沈阳建筑大学 | Preparation method and application for compound fungus agent for degrading organic matter |
| CN102583782A (en) * | 2012-03-27 | 2012-07-18 | 宁夏大学 | Method for degrading phenol in waste water in coal chemical industry |
| CN103748214A (en) * | 2011-07-13 | 2014-04-23 | 生物地带有限公司 | Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same |
| CN103931659A (en) * | 2014-04-30 | 2014-07-23 | 武汉虹睿生物科技开发有限公司 | Application of bacillus mojavensis KJS-3 as biopesticide |
| CN103960461A (en) * | 2014-04-24 | 2014-08-06 | 武汉虹睿生物科技开发有限公司 | Application of adopting Mojave Bacillus KJS-3 as feed additive |
| CN105255758A (en) * | 2015-10-15 | 2016-01-20 | 国家海洋局第三海洋研究所 | Application of Sulfobacillus acidophilus TPY in phenol pollutant degradation and method for degrading phenol pollutants through Sulfobacillus acidophilus TPY |
-
2016
- 2016-06-06 CN CN201610415649.9A patent/CN106673204A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974459A (en) * | 2010-10-12 | 2011-02-16 | 黑龙江省科学院微生物研究所 | Microbes capable of degrading phenol and cyanogen in coking waste water and method for treating coking waste water by using same |
| CN103748214A (en) * | 2011-07-13 | 2014-04-23 | 生物地带有限公司 | Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same |
| CN102344899A (en) * | 2011-10-21 | 2012-02-08 | 沈阳建筑大学 | Preparation method and application for compound fungus agent for degrading organic matter |
| CN102583782A (en) * | 2012-03-27 | 2012-07-18 | 宁夏大学 | Method for degrading phenol in waste water in coal chemical industry |
| CN103960461A (en) * | 2014-04-24 | 2014-08-06 | 武汉虹睿生物科技开发有限公司 | Application of adopting Mojave Bacillus KJS-3 as feed additive |
| CN103931659A (en) * | 2014-04-30 | 2014-07-23 | 武汉虹睿生物科技开发有限公司 | Application of bacillus mojavensis KJS-3 as biopesticide |
| CN105255758A (en) * | 2015-10-15 | 2016-01-20 | 国家海洋局第三海洋研究所 | Application of Sulfobacillus acidophilus TPY in phenol pollutant degradation and method for degrading phenol pollutants through Sulfobacillus acidophilus TPY |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mirizadeh et al. | Biodegradation of cyanide by a new isolated strain under alkaline conditions and optimization by response surface methodology (RSM) | |
| Afzal et al. | Characteristics of phenol biodegradation in saline solutions by monocultures of Pseudomonas aeruginosa and Pseudomonas pseudomallei | |
| Banerjee et al. | Interlining Cr (VI) remediation mechanism by a novel bacterium Pseudomonas brenneri isolated from coalmine wastewater | |
| Zhu et al. | Isolation and application of a chlorpyrifos-degrading Bacillus licheniformis ZHU-1 | |
| Prasad et al. | Physicochemical analysis of textile effluent and decolorization of textile azo dye by Bacillus Endophyticus strain VITABR13 | |
| CN110643548B (en) | Microbacterium flavum for degrading aniline and application thereof | |
| CN101857847A (en) | Pseudomonas aeruginosa strain separating, purifying and domesticating method and use | |
| Joshi et al. | Screening and characterization of heavy metal resistant bacteria for its prospects in bioremediation of contaminated soil | |
| El-Sayed et al. | Multiple heavy metal and antibiotic resistance of Acinetobacter baumannii strain HAF–13 isolated from industrial effluents | |
| Moradkhani et al. | Biodegradation of cyanide under alkaline conditions by a strain of Pseudomonas putida isolated from gold mine soil and optimization of process variables through response surface methodology (RSM) | |
| Zhu et al. | The endophytic bacterium Serratia sp. PW7 degrades pyrene in wheat | |
| Feng et al. | Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L. | |
| Arous et al. | Treatment of olive mill wastewater through employing sequencing batch reactor: performance and microbial diversity assessment | |
| Wang et al. | Sulfamethoxazole degradation by Aeromonas caviae and co-metabolism by the mixed bacteria | |
| Farag et al. | Degradation of phenol by a new-degradable marine halophilic fungus Fennellia flavipes isolated from mangrove sediments | |
| Mehanni et al. | Antibiotic-resistant bacteria in hospital wastewater treatment plant effluent and the possible consequences of its reuse in agricultural irrigation | |
| Karunya et al. | Efficiency of bacterial isolates in the degradation of malathion and parathion | |
| Kumkaew et al. | Biodecolorization of biogas plant effluent derived from anaerobically digested distillery wastewater by naturally selected Pseudomonas putida | |
| Teerakun et al. | Optimization of paraquat degradation with microbial consortium from contaminated soil using statistic method | |
| CN107164277A (en) | A kind of Halomonas of degradation of phenol | |
| Dey et al. | Studies on biodegradation of 4-chlorophenol and 4-nitrophenol by isolated pure cultures | |
| CN106673204A (en) | Application of bacillus mojavensis KJS-3 to decomposition of phenol | |
| Kausar et al. | Efficacy of Bacterial Strains Isolated from Textile Wastewater for Degradation of Azo Dye Associated Aromatic Amines. | |
| Thimmarayan et al. | Biodegradation of naphthalene–Ecofriendly approach for soil pollution mitigation | |
| Chaturvedi et al. | Multiple antibiotic resistance patterns of rhizospheric bacteria isolated from Phragmites australis growing in constructed wetland for distillergy effluent treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170517 |