CN103451127B - Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof - Google Patents
Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof Download PDFInfo
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- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical group CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 title claims abstract description 109
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 51
- 230000015556 catabolic process Effects 0.000 title claims abstract description 49
- 241000123669 Zoogloea resiniphila Species 0.000 title claims abstract description 11
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims abstract description 23
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000002957 persistent organic pollutant Substances 0.000 claims abstract description 12
- 230000000593 degrading effect Effects 0.000 claims abstract description 11
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims description 38
- 239000003292 glue Substances 0.000 claims description 21
- 239000002028 Biomass Substances 0.000 claims description 15
- 230000033558 biomineral tissue development Effects 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 6
- 239000010865 sewage Substances 0.000 claims description 5
- 230000001413 cellular effect Effects 0.000 claims description 4
- 239000002351 wastewater Substances 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- MVPPADPHJFYWMZ-UHFFFAOYSA-N chlorobenzene Chemical compound ClC1=CC=CC=C1 MVPPADPHJFYWMZ-UHFFFAOYSA-N 0.000 abstract description 12
- 239000003344 environmental pollutant Substances 0.000 abstract description 4
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 150000001555 benzenes Chemical class 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 31
- 241000589651 Zoogloea Species 0.000 description 21
- 235000015097 nutrients Nutrition 0.000 description 10
- 238000000034 method Methods 0.000 description 8
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 230000008859 change Effects 0.000 description 6
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- 238000005516 engineering process Methods 0.000 description 5
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- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
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- 238000004458 analytical method Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical group CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- LGRFSURHDFAFJT-UHFFFAOYSA-N Phthalic anhydride Natural products C1=CC=C2C(=O)OC(=O)C2=C1 LGRFSURHDFAFJT-UHFFFAOYSA-N 0.000 description 2
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 2
- 108010021006 Tyrothricin Proteins 0.000 description 2
- 125000005605 benzo group Chemical group 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- JHIWVOJDXOSYLW-UHFFFAOYSA-N butyl 2,2-difluorocyclopropane-1-carboxylate Chemical compound CCCCOC(=O)C1CC1(F)F JHIWVOJDXOSYLW-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
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- BGHCVCJVXZWKCC-UHFFFAOYSA-N tetradecane Chemical compound CCCCCCCCCCCCCC BGHCVCJVXZWKCC-UHFFFAOYSA-N 0.000 description 2
- 231100001234 toxic pollutant Toxicity 0.000 description 2
- GSXRBRIWJGAPDU-BBVRJQLQSA-N tyrocidine A Chemical compound C([C@H]1C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@H](C(N[C@H](CC=2C=CC=CC=2)C(=O)N2CCC[C@H]2C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N1)=O)CC(C)C)C(C)C)C1=CC=C(O)C=C1 GSXRBRIWJGAPDU-BBVRJQLQSA-N 0.000 description 2
- 229960003281 tyrothricin Drugs 0.000 description 2
- 239000002912 waste gas Substances 0.000 description 2
- KMOUUZVZFBCRAM-OLQVQODUSA-N (3as,7ar)-3a,4,7,7a-tetrahydro-2-benzofuran-1,3-dione Chemical compound C1C=CC[C@@H]2C(=O)OC(=O)[C@@H]21 KMOUUZVZFBCRAM-OLQVQODUSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical class N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010012218 Delirium Diseases 0.000 description 1
- 206010012373 Depressed level of consciousness Diseases 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
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- 241000187654 Nocardia Species 0.000 description 1
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- HGCIXCUEYOPUTN-UHFFFAOYSA-N cyclohexene Chemical compound C1CCC=CC1 HGCIXCUEYOPUTN-UHFFFAOYSA-N 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof in an ortho-xylene organic pollutant. The collection number of the Zoogloearesiniphila HJ1 is CCTCC NO. M2012235. The invention also provides application of the Zoogloearesiniphila HJ1 with the ortho-xylene degradation capacity in degrading organic pollutants, namely benzene, methylbenzene, ethylbenzene, dimethylbenzene and chlorobenzene. The Zoogloearesiniphila HJ1 disclosed by the invention can be used for efficiently degrading the benzene series pollutants, such as ortho-xylene, and is high in tolerance capacity.
Description
Technical field
The invention belongs to environmental pollutant biologic treating technique field, be specifically related to a strain have o-Xylol degradation capability moving glue bacterium (
zoogloea resiniphila) HJ1 and application thereof.
Background technology
O-Xylol (C
8h
10,
o-xylene) be the industrial chemicals of producing phthalic anhydride (Tetra hydro Phthalic anhydride, PA), dyestuff, agrochemical, wherein 90% left and right is for the production of phthalic anhydride.Its steam and air form explosive mixture, and chance naked light, high heat energy cause combustion explosion.Its steam is heavier than air, and can be diffused in lower place quite far away, meets burning things which may cause a fire disaster and is drawing and strile-back.If meet high heat, in container, press and increase, there is the danger of cracking and blast.If flow velocity is too fast, easily produce and gather static.Skin, mucous membrane are had to hormesis, central nervous system is had to anesthetic action; Long term can affect liver, renal function.Acute poisoning: patient has the severe persons such as cough, stream are prevented, conjunctival congestion to have illusion, delirium, obnubilation etc., the hysteria sample that has having shows effect.O-Xylol is classified as one of 129 kinds of preferential toxic pollutants of controlling by Environmental Protection Agency (EPA).Therefore the purification techniques of studying o-Xylol just seems very urgent and important.
Biopurification technology has the features such as removal efficiency is high, processing costs is low, secondary pollution is little, is widely used in gradually degraded and the purification of toxic pollutant.One of key that adopts biotechnology processing o-Xylol is to obtain the bacterial strain with efficient degradation o-Xylol ability.At present, Chinese scholars has been carried out large quantity research to the biological degradation of benzene compounds, the o-Xylol degradation bacteria being separated to so far mainly comprise rhodococcus (
rhodococcus), pseudomonas (
pseudomonas) etc.Large quantity research shows the efficient o-Xylol degradation bacteria of separation screening from environment, remains one of important method of eliminating BETX pollutent in environment.
Moving glue bacterium in the present invention (
zoogloea resiniphila) be a kind of common tyrothricin, be 201010249613.0 in the patent No., the Chinese patent that patent name is " for composite and highly effective microorganism preparation and the synergistic process of synergy of sewage treatment plant " discloses a kind of compound formulation that uses moving glue bacterium, makes from the multiple mushroom of hair Zymomonas mobilis, genus bacillus, pseudomonas, Alcaligenes and Nocardia, for the treatment of various sewage, especially high saliferous and high-ammonia-nitrogen sewage.Through patent searching and other pertinent literatures, not yet find to utilize the report of this strain degradation o-Xylol.The discovery of this degradation bacteria is significant for the high-efficient purification of the benzo pollutants such as BETX in trade effluent waste gas.
Summary of the invention
Technical problem to be solved by this invention is to overcome deficiency of the prior art and a kind of efficient, moving glue bacterium and the application thereof with o-Xylol degradation capability that tolerance is strong are provided.
The technical scheme that the present invention solves the problems of the technologies described above employing is: a strain have o-Xylol degradation capability moving glue bacterium (
zoogloea resiniphila) HJ1, the GenBank number of logging in of bacterial strain 16S rDNA is JQ751310, this bacterial strain is preserved in Chinese Typical Representative thing preservation center, address: and Wuhan, China Wuhan University, 430072, deposit number is: CCTCC NO. M2012235.Being characterized as of this bacterial strain: bacterium colony is white in color, neat in edge, smooth moistening.The form of observing this thalline under transmission electron microscope is tyrothricin, amphitrichous, Gram-negative, oxidase positive.
The invention provides the moving glue bacterium HJ1 with o-Xylol degradation capability in the application of degrading in o-Xylol organic pollutant.
The present invention also provides the moving glue bacterium HJ1 with o-Xylol degradation capability in the application of degrading in benzene, toluene, ethylbenzene, dimethylbenzene, chlorobenzene organic pollutant.
The present invention puts into the described moving glue bacterium HJ1 with o-Xylol degradation capability in the sewage containing o-Xylol through cultivating the bacteria suspension obtaining, and is 25-35 DEG C in temperature, under the condition of pH value for 7-9, cultivates.
Moving glue bacterium HJ1 optimum culturing temperature in waste water with o-Xylol degradation capability of the present invention is 35 DEG C, pH7, and o-Xylol degradation rate is 5.33 mg/ (Lh).
The moving glue bacterium HJ1 degraded o-Xylol concentration range with o-Xylol degradation capability of the present invention is 0-256 mg/L, and high specific degradation rate is 0.19 mg o-Xylol/(mg cellsh).
The moving glue bacterium HJ1 with o-Xylol degradation capability of the present invention is finally converted into CO o-Xylol
2, H
2o and cellular biomass, its average yield coefficient is 0.2572 mg cells/mg o-Xylol, average mineralization rate is 54.8%.
The present invention compared with prior art has the following advantages: can realize the high-efficient purification of benzo pollutants, the especially o-Xylol such as BETX in trade effluent waste gas, and not produce any secondary pollution.Easily promote, purify cost low.
Brief description of the drawings
Fig. 1 is
zoogloea resiniphilathe gramstaining photo of HJ1.
Fig. 2 is
zoogloea resiniphilathe transmission electron microscope photo of HJ1.
Fig. 3 is
zoogloea resiniphilathe phylogeny tree graph of HJ1.
Fig. 4 is
zoogloea resiniphilacellular biomass change curve in the degradation curve of HJ1 to 32mg/L, 64 mg/L, 96 mg/L, 128 mg/L, 160 mg/L o-Xylols, degradation process.
Fig. 5 is
zoogloea resiniphilacellular biomass change curve in the degradation curve of HJ1 to 192 mg/L, 224 mg/L, 256 mg/L, 288 mg/L o-Xylols, degradation process.
Fig. 6 is
zoogloea resiniphilahJ1 is to the matched curve of o-Xylol specific degradation rate.
Fig. 7 is
zoogloea resiniphilahJ1 is CO in the process of degraded o-Xylol
2growing amount matched curve.
Fig. 8 is
zoogloea resiniphilahJ1 is cellular biomass formation curve in the process of degraded o-Xylol.
Fig. 9 is
zoogloea resiniphilahJ1 is to the common organic pollutant degradation performance analysis of industry.
Embodiment
The experimental technique using in following embodiment if no special instructions, is ordinary method.
Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.
Embodiment mono-,
zoogloea resiniphilaseparation, purifying and the qualification thereof of HJ1.
1.
zoogloea resiniphilathe separation of HJ1 and purifying.
zoogloea resiniphilahJ1 is the strain Gram-negative bacteria that domestication from the active sludge of Xinchang waste water in pharmaceutical plants treatment station, separation obtain.Concrete steps are as follows:
Get the active sludge of Xinchang waste water in pharmaceutical plants treatment station, tap water is eluriated after five times, and the sky 48h that exposes to the sun removes residual organism as far as possible.Preparation inorganic salt nutrient solutions (pH7), carry out orientation domestication taking o-Xylol as sole carbon source to active sludge, and 2 d change fresh inorganic salt nutrient solution, measure the pH of nutrient solution every day.Preparation o-Xylol starting point concentration is 100 mg/L minimal mediums, and compound method is as follows: 1g/L KH
2pO
4, 4.5g/L Na
2hPO
4, 0.5g/L (NH
4)
2sO
4, 0.1g/L MgSO
4, 0.02g/L CaCl
2, 0.1mg/L MnSO
44H
2o, 0.1mg/L ZnSO
47H
2o, 0.02mg/L CuSO
45H
2o, 0.02mg/L CoCl
26H
2o, 0.02 mg/L Na
2moO
42H
2o, 1mg/L FeSO
47H
2o, 0.02mg/L H
3bO
3, pH 7.3 ~ 7.55.Be sub-packed in the sealing saline bottle (50 mL/) of 250 mL, 110 DEG C of sterilizing 40 min.After substratum is cooling, add o-Xylol as sole carbon source, the mud of the domestication amount that by volume mark is 5% is joined in substratum, in 30 DEG C, the shaking table shaking culture of 160 rpm.3d left and right is transferred in the minimal medium after fresh sterilizing with 10% amount, shaking table shaking culture.Separate in conjunction with agar inorganic salt plate streaking, picking list bacterium colony access minimal medium shaking table continues shaking culture, until obtain single bacterium of the degradable o-Xylol that growth is fast in above-mentioned substratum, bacterium colony is regular and proterties is stable.
2.
zoogloea resiniphilathe qualification of HJ1
By 16S rDNA sequential analysis and Physiology and biochemistry experimental identification, determine that bacterial strain HJ1 is
zoogloea resiniphila.Concrete steps are as follows:
Adopt the DNA of the centrifugal environmental sample DNA recovery test kit of 3S post (V2.2, Shanghai Shenergy Biocolor BioScience & Technology Company) extraction and purifying bacterial strain, 4 DEG C of preservations.Select universal primer BSF8/20 and the BSR1541/20 of bacterium to carry out pcr amplification to the DNA of purifying, primer sequence is respectively:
BSF8/20：5'-AGAGT TTGAT CCTGG CTCAG-3'
BSR1541/20：5'-AAGGA GGTGA TCCAG CCGCA-3'
PCR response procedures is set as: 94 ° of C denaturation 4 min; Then 94 ° of C sex change 1 min, 59 ° of C, 1 min that anneals, 72 ° of C extend 1.5 min, circulate 35 cycles; Then 72 ° of C extend 10 min; Last 4 ° of C keep 10 min.PCR product is checked order (the prompt base in the English Weihe River, Shanghai), sequencing result is shown in sequence table.
The 16S rDNA sequence of bacterial strain HJ1 is uploaded to Genbank, obtain the accession number JQ751310 of Genbank, carry out homology comparison with the gene order in Genbank, find that it belongs to simultaneously
zoogloeabelong to, with
zoogloea resiniphilastrain DhA-35(NR027188) homology is the highest, reaches 99%.By this bacterial strain called after
zoogloea resiniphilhJ1, and (address: Wuhan, China Wuhan University, postcode 430072) preservation at Chinese Typical Representative culture collection center on June 18th, 2012, preserving number is CCTCC NO.M2012235.
Embodiment bis-,
zoogloea resiniphilahJ1 detects the degradation property of different concns o-Xylol.
Under optimum environment factor condition (nutrient solution pH7,35 DEG C of culture temperature), investigate the degradation property of bacterial strain LX-1 to starting point concentration 32-288mg/L o-Xylol.Result shows, the degradable 32-256 mg/L o-Xylol of bacterial strain HJ1 energy, but can only Partial digestion starting point concentration be 288 mg/L o-Xylols.Under optimal culture condition, bacterial strain high specific degradation rate has reached 0.19 mg
o-xylene/(mg cellsh).Concrete implementation step is as follows:
Inorganic salt nutrient solution 1000 mL of preparation pH=7, are sub-packed in the saline bottle of 250mL every bottle of 50mL, 110 DEG C of sterilizing 40 min.After nutrient solution is cooling, get wherein 10 bottles and add the bacteria suspension in the HJ1 of logarithmic phase, add o-Xylol as sole carbon source, make its concentration reach 32,64,128,160,192,224,256,288 mg/L; Blank is set.Saline bottle seals rear 35 DEG C of shaking culture, and o-Xylol concentration and biomass residual in nutrient solution are measured in timing, draws bacterial strain HJ1 for different starting point concentration o-Xylols degradation curve very, and calculates corresponding specific degradation rate.
Fig. 4 ~ 6 are that bacterial strain HJ1 is with the degradation curve without starting point concentration o-Xylol.Result shows, when o-Xylol concentration is during lower than 256 mg/L, bacterial strain HJ1 can be degradable; But bacterial strain HJ1 fails to realize degradable to 288 mg/L o-Xylols, and after 132 h, its residual concentration is about 113.5 mg/L.When o-Xylol concentration is during lower than 160 mg/L, the biomass of bacterial strain HJ1 is along with the concentration of o-Xylol raises and increases; In the time that the concentration of o-Xylol is 160-224 mg/L, the biomass of bacterial strain remains unchanged, and maintains 46 mg/L left and right; In the time that the concentration of o-Xylol rises to 256 mg/L, the biomass of bacterial strain drops to 36.6 mg/L; In the time that o-Xylol concentration further rises to 288 mg/L, the biomass of bacterial strain further drops to 15.5 mg/L.Pass through Haldane ' s inhibition growth kinetics model simultaneously specific degradation rate has been carried out to matching, obtained high specific degradation rate.In the time that o-Xylol concentration is 20 mg/L, o-Xylol specific degradation rate reaches maximum value 0.19h
-1.
Embodiment tri-,
zoogloea resiniphilahJ1 is to the mineralization rate of o-Xylol and average yield coefficient analysis.
Under optimum environment factor condition (nutrient solution pH7,35 DEG C of culture temperature), investigate bacterial strain
zoogloea resiniphilahJ1 is to the mineralization rate of initial o-Xylol concentration 32-258 mg/L and average yield coefficient.Result shows, described bacterial strain HJ1 can finally be converted into CO o-Xylol
2, H
2o and cellular biomass, average mineralization rate is 54.8%, average yield coefficient is 0.2572 mg cells/mg
o-xylene.Concrete implementation step is as follows:
In degraded substratum by bacterial suspension inoculation in 32,64,92,128,160,192,224,256 and 288 mg/L taking o-Xylol as sole carbon source, sealing is cultivated under 35 DEG C, pH=7.5 condition.Measure initial bacteria concentration and CO in culturing bottle
2concentration.Then, add hydrochloric acid wait degrading when complete, leave standstill half an hour, then measure final bacteria concentration and CO
2concentration.According to CO
2the changing conditions of concentration is calculated the mineralization rate of o-Xylol, according to the yield coefficient of bacteria concentration change calculations strains for degrading o-Xylol.
Result is as shown in Fig. 7 ~ 8.When o-Xylol concentration is during lower than 192 mg/L, CO
2growing amount and the linear growth of o-Xylol consumption; When o-Xylol concentration is during higher than 192 mg/L, CO
2growing amount tends towards stability.When o-Xylol concentration is during lower than 192 mg/L, CO
2it is y=1.821x that the matching of growing amount and o-Xylol consumption is closed, and this is CO
2the ratio of growing amount and o-Xylol consumption, consumes 1mg o-Xylol and can produce 1.821mgCO
2.In theory, when o-Xylol is entirely H
2o and CO
2time, CO
2the pass of growing amount and o-Xylol consumption is y=3.32x.When this shows that o-Xylol is degradable by bacterial strain HJ1, the mineralization rate of C is 54.8%.When o-Xylol concentration is during lower than 192 mg/L, biomass and the linear growth of o-Xylol consumption; When toluene concentration is during higher than 192 mg/L, biomass sharply declines.When toluene concentration is during lower than 192 mg/L, bacterial strain concentration consumes concentration degree of fitting (R to toluene
2) being greater than 0.99, the slope of straight line is cell yield coefficient, is 0.2572 mg cells/mg
o-xylene.
Embodiment tetra-,
zoogloea resiniphilahJ1 is to the common organic pollutant degradation performance analysis of industry
In the industry such as benzene, toluene, ethylbenzene, chlorobenzene, common are organic pollutants as sole carbon source respectively, investigate bacterial strain
zoogloea resiniphilahJ1 is for the degradation capability of these materials.Result shows, the bacterial strain HJ1 above-mentioned organic pollutant of degrading to some extent, comparatively fully degrading benzene thing, to the degradation rate of chlorobenzene, hexanaphthene etc. at 8-42%.Specific embodiments is as follows:
Preparation pH is 7.3, inorganic salt nutrient solution 800 mLs of salt concn (taking NaCl) as 0.66%, is sub-packed in the saline bottle of 250 mL every bottle of 50 mL, 110 DEG C of sterilizing 40 min.After nutrient solution is cooling, get wherein 8 bottles and add the bacteria suspension in logarithmic phase HJ1, make initial biomass reach 38.4 mg/L(in wet thallus), using benzene, toluene, ethylbenzene, chlorobenzene etc. as sole carbon source, starting point concentration is 100 mg/L respectively; Separately getting 8 bottles adds the above-mentioned organism of same amount but does not add HJ1 bacteria suspension as blank.Saline bottle sealing is placed on the shaking table shaking culture of 33 DEG C, analyzes residual organic concentration in liquid phase respectively at 48h, measures corresponding biomass, calculates the degradation rate of each material.
Result as shown in Figure 9.The molecular structure of organic pollutant, accept or provide the ability of electronics to determine its chemical stability, and affecting microorganism to organic absorption and transfer ability, thereby determining organic biodegradable.Because BETX has the structure of phenyl ring, bacterial strain HJ1 has relevant oxygenase, can be by BETX catalyzed degradation effectively.Bacterial strain HJ1 can not degrade and have the chlorobenzene of benzene ring structure, may be because the Cl on phenyl ring makes electrophilic substitution reaction passivation.And methyl halide, the tetradecane, sherwood oil (main component is pentane and hexane), the structure of the compounds such as hexanaphthene and tetrahydrobenzene and the difference of BETX are larger, therefore bacterial strain HJ1 can not carry out degradable to it.Above test-results explanation, bacterial strain
zoogloea resiniphilathe HJ1 multiple common organic pollutant of degrading, has certain directive significance for this bacterial strain for actual industrial Pollution abatement.
Although the present invention with embodiment openly as above; but it is not in order to limit protection scope of the present invention; any technician who is familiar with this technology, not departing from change and the retouching done in the spirit and scope of the present invention, all should belong to protection scope of the present invention.
<110> Zhejiang Polytechnical University
<120> mono-strain has moving glue bacterium and the application thereof of o-Xylol degradation capability
<160> 1
<210>1
<211>1440
<212> DNA
<213> move glue bacterium (
zoogloea resiniphila)
<400>1
ttggggcggc agctttccat gcaagtcgaa cggcagcacg ggcttcggcc tggtggcgag <60>
tggcgaacgg gtgagtaatg catcggaacg tacccagtcg tgggggataa cgtagcgaaa <120>
gttacgctaa taccgcatac gtcctgaggg agaaagcggg ggaccgtaag gcctcgcgcg <180>
attggagcgg ccgatgtcgg attagctagt tggtggggta aaggcctacc aaggcgacga <240>
tccgtagcgg gtctgagagg atgatccgcc acactgggac tgagacacgg cccagactcc <300>
tacgggaggc agcagtgggg aattttggac aatgggcgaa agcctgatcc agccatgccg <360>
cgtgagtgaa gaaggccttc gggttgtaaa gctctttcag acggaaagaa atcttctggg <420>
ctaataccct gggaggatga cggtaccgta agaagaagca ccggctaact acgtgccagc <480>
agccgcggta atacgtaggg tgcgagcgtt aatcggaatt actgggcgta aagcgtgcgc <540>
aggcggtgat gtaagacaga tgtgaaatcc ccgggctcaa cctgggaact gcgtttgtga <600>
ctgcatcact cgagtacggc agagggaggt ggaattccgc gtgtagcagt gaaatgcgta <660>
gagatgcgga ggaacaccga tggcgaaggc agcctcctgg gccagtactg acgctcatgc <720>
acgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccc taaacgatgt <780>
caactagttg ttcggtgagg agactcattg agtaacgcag ctaacgcgtg aagttgaccg <840>
cctggggagt acggccgcaa ggttaaaact caaaggaatt gacggggacc cgcacaagcg <900>
gtggatgatg tggattaatt cgatgcaacg cgaaaaacct tacctaccct tgacatgcca <960>
ggaacttgcc agagatggct tggtgctcga aagagagcct ggacacaggt gctgcatggc <1020>
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgtc <1080>
attagttgcc atcattaagt tgggcactct aatgagactg ccggtgacaa accggaggaa <1140>
ggtggggatg acgtcaagtc ctcatggccc ttatgggtag ggcttcacac gtcatacaat <1200>
ggtcggtaca gagggttgcc aagccgcgag gtggagccaa tcccagaaag ccgatcgtag <1260>
tccggattgg agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgcagatca <1320>
gcatgctgcg gtgaatacgt tcccgggtct tgtacacacc gcccgtcaca ccatgggagt <1380>
ggggtttacc agaagtaggt agcttaaccg caaggagggc gctaccacgt agctcgtccc <1440>
Claims (7)
- One strain have o-Xylol degradation capability moving glue bacterium ( zoogloea resiniphila) HJ1, its deposit number is CCTCC NO.M2012235.
- 2. the moving glue bacterium HJ1 with o-Xylol degradation capability claimed in claim 1 is in the application of degrading in o-Xylol organic pollutant.
- 3. the moving glue bacterium HJ1 with o-Xylol degradation capability claimed in claim 1 is in the application of degrading in benzene, toluene, ethylbenzene, dimethylbenzene organic pollutant.
- 4. application according to claim 2, is characterized in that: the described moving glue bacterium HJ1 with o-Xylol degradation capability is put in the sewage containing o-Xylol through cultivating the bacteria suspension obtaining, and is 25-35 DEG C in temperature, under the condition of pH value for 7-9, cultivates.
- 5. application according to claim 4, is characterized in that: described in there is o-Xylol degradation capability moving glue bacterium HJ1 optimum culturing temperature in waste water be 35 DEG C, pH7, o-Xylol degradation rate is 5.33 mg/ (Lh).
- 6. application according to claim 5, it is characterized in that: the described moving glue bacterium HJ1 degraded o-Xylol concentration range with o-Xylol degradation capability is 0-256 mg/L, and high specific degradation rate is 0.19 mg o-Xylol/(mg cellsh).
- 7. application according to claim 5, is characterized in that: the described moving glue bacterium HJ1 with o-Xylol degradation capability is finally converted into CO o-Xylol 2, H 2o and cellular biomass, its average yield coefficient is 0.2572 mg cells/mg o-Xylol, average mineralization rate is 54.8%.
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