CN106361683B - A kind of production method of pet special bio bacterium taste removal care agent - Google Patents

A kind of production method of pet special bio bacterium taste removal care agent Download PDF

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CN106361683B
CN106361683B CN201610754181.6A CN201610754181A CN106361683B CN 106361683 B CN106361683 B CN 106361683B CN 201610754181 A CN201610754181 A CN 201610754181A CN 106361683 B CN106361683 B CN 106361683B
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pet
care agent
taste removal
production method
probiotics
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CN106361683A (en
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黄胜威
吴丽芳
潘高志
高战元
蔡冬清
吴正岩
段子渊
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Hefei Institutes of Physical Science of CAS
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Abstract

The invention discloses a kind of production methods of pet special bio bacterium taste removal care agent, comprising the following steps: the fermentation of probiotics single strain and the acquisition of zymocyte liquid, the preparation of probiotics composite bacteria liquid, the addition of fresh culture, protectant addition.The present invention has the advantages that fundamentally the harmful organic contaminants with degradation pet, make its fur keep automatically cleaning and free from extraneous odour (1) by the synergistic effect between probiotics strain in care agent；(2) the elimination of harmful bacteria with pet is acted on by probiotics, play the double effects of disinfection and deodorization；(3) probiotics licks through pet and licks, and into after pet's oral cavity and enteron aisle, can further maintain the oral health and intestinal flora balance of pet, improve the utilization rate of food, enhancing love dotes on immunity, and love is effectively reduced and dotes on oral cavity and excreta peculiar smell and prevents to decay tooth, plays good health-care effect.

Description

A kind of production method of pet special bio bacterium taste removal care agent
Technical field
The present invention relates to microbe technical field more particularly to a kind of systems of pet special bio bacterium taste removal care agent Make method
Background technique
Pet is the purpose for spirit and the animals and plants fed, and the family of pet-feeding is increasing at present.Animal class Pet is especially doggie, the peculiar smell with kitten, is the problem for perplexing pet fan's many years.Peculiar smell with pet, it is main Filthy composition caused by wanting source is itself secretion of pet, rolling of playing is climbed is decomposed under that action of microbes such as bacteria and is produced Raw.To increase, raising pet is entrusted to the care of to mankind's bring emotion and pleasant feeling, the regular disinfecting, deodorizing of pet just seem very heavy It wants.
Pet disinfection deodorizer currently on the market, mostly with chlorine class preparation (including inorganic chloride, such as 84 disinfections Agent, sodium hypochlorite, bleaching powder；Organochlorine compound, such as sodium dichloro cyanurate, sodium trichloro-isocyanurate) and aromatic be It is main.Dog nature are liked smelling everywhere to smell, when in smelling of bowing man everywhere, meeting very close contact disinfectant.If selected Irritation disinfectant, love are easy in sucking alveolar when doting on smelling, will lead to chronic injury.Cat love, which is licked, licks hair, with contamination Thimerosal ingredient just have the possibility licked.They, which are different from our mankind, can select far from harmful disinfectant, so dog Requirement of the cat to disinfectant is than people Geng Gao, and general aromatic or conventional deodorizers are for pet deodorization and improper.
Therefore, safe and nontoxic, fundamentally deodorization pure biological taste removal care agent is selected, becomes more and more pets and raises It supports the family the selection in front yard.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of production side of pet special bio bacterium taste removal care agent Method.
In order to solve the above technical problems, the present invention provides a kind of production method of pet special bio bacterium taste removal care agent, The following steps are included:
1, a kind of production method of pet special bio bacterium taste removal care agent, which comprises the steps of:
(1): the fermentation of probiotics single strain
The composition of the probiotics single plant bacterial strain is as follows:
Bacillus licheniformis Bacillus Lincheniforms (living bacteria count: 1.0 × 109-3.0×109cfu/ ML), bacillus subtilis Bacillus Subtilis (living bacteria count: 5.0 × 109-8.0×109Cfu/mL), pentose piece ball Bacterium Pediococcus pentosaceus (living bacteria count: 4.0 × 109-7.0×109Cfu/mL), lactobacillus acidophilus Lactobacillus acidophilus (living bacteria count: 6.0 × 109-8.0×109Cfu/mL), brewer's yeast Sacchaeomyces cerevisiae (living bacteria count: 1.0 × 109-2.0×109) and streptococcus fecalis cfu/mL Streptococcus faecalis (living bacteria count: 2.0 × 109-3.0×109cfu/mL)；
By bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, lactobacillus acidophilus, brewer's yeast and streptococcus fecalis Six plants of bacterial strains individually ferment, and obtain corresponding zymocyte liquid；
(2): the preparation of probiotics composite bacteria liquid
After fermentation to above-mentioned each single strain, by bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, acidophilus Lactobacillus, six plants of bacterial strain fresh fermentation broths of brewer's yeast and streptococcus fecalis according to 1-3:5-8:4-7:6-8:1-2:2-3 volume Than mixing, probiotics composite bacteria liquid is made, wherein living bacteria count is 1.9 × 1010-3.9×1010cfu/mL；
(3): the addition of fresh culture
Fresh cultured matrix-cane molasses are added into above-mentioned composite bacteria liquid by mass concentration 0.5%-1.5%；
(4): protectant addition
The potassium sorbate that purity is 98%-100% is added into liquid obtained in (3) by mass concentration 1-1.5 ‰, in In the shaking table of 100-150rpm revolving speed, uniform stirring 1-2h.
Preferably, the bacillus licheniformis and bacillus subtilis single strain fermentation the following steps are included:
The bacillus licheniformis and bacillus subtilis are cultivated into 24-36h, inoculum concentration 1%- in LBG culture medium 2%, shaking speed 180-200rpm.
Preferably, the formula of the LBG culture medium are as follows: glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, Pure water 1000mL.
Preferably, the Pediococcus pentosaceus and lactobacillus acidophilus fermentation the following steps are included:
The Pediococcus pentosaceus and lactobacillus acidophilus are cultivated into 24-36h, inoculum concentration 1%-2% in MRS culture medium, Shaking speed is 180-200rpm.
Preferably, the formula of the MRS culture medium are as follows: peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000mL.
Preferably, the brewer's yeast fermentation the following steps are included:
The brewer's yeast is cultivated into 24-36h, inoculum concentration 1%-2%, shaking speed 180- in YPD culture medium 200rpm。
Preferably, the formula of the YPD culture medium are as follows: yeast extract 10g, peptone 20g, glucose 20g, pure water 1000mL。
Preferably, the streptococcus fecalis fermentation the following steps are included:
The streptococcus fecalis is cultivated into 24-36h, inoculum concentration 1%-2%, shaking speed 180- in nutrient medium 200rpm。
Preferably, the formula of the nutrient medium are as follows: sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, sodium chloride 2g, magnesium sulfate 0.2g, pure water 1000mL.
Preferably, the sugar content of the fresh cultured matrix-cane molasses is 50%-100%, and brix (Bx) is 85%-100%.
The present invention has the advantages that the biological bacteria taste removal care agent includes bacillus, Bacillus acidi lactici, saccharomycete and chain Coccus, harmful organic substance, organic contamination by the synergistic effect between probiotics strain, with pet of fundamentally degrading Object, so that the fur of pet be made to keep automatically cleaning and free from extraneous odour.Meanwhile the probiotics in care agent can also kill harmful bacteria, Play the double effects of disinfection and deodorization.Importantly, the probiotics in biological bacteria taste removal care agent licks through pet and licks, enter After pet's oral cavity and enteron aisle, moreover it is possible to further maintain pet's oral cavity health and intestinal flora balance, improve the utilization rate of food, increase Strong love dotes on immunity, and love is effectively reduced and dotes on oral cavity and excreta peculiar smell and prevents to decay tooth.
Detailed description of the invention
Fig. 1 is a kind of stream of the production method for pet special bio bacterium taste removal care agent that 1-3 of the embodiment of the present invention is provided Cheng Tu.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation Example.
Embodiment 1
A kind of production method of pet special bio bacterium taste removal care agent, as shown in Figure 1, including the following steps:
(1): the fermentation of probiotics single strain
The composition of the probiotics single plant bacterial strain is as follows:
Bacillus licheniformis Bacillus Lincheniforms (living bacteria count: 1.0 × 109Cfu/mL), withered grass bud Spore bacillus Bacillus Subtilis (living bacteria count: 5.0 × 109Cfu/mL), Pediococcus pentosaceus Pediococcus Pentosaceus (living bacteria count: 4.0 × 109Cfu/mL), lactobacillus acidophilus Lactobacillus acidophilus (has Effect viable count: 6.0 × 109Cfu/mL), brewer's yeast Sacchaeomyces cerevisiae (living bacteria count: 1.0 × 109) and streptococcus fecalis Streptococcus faecalis (living bacteria count: 2.0 × 10 cfu/mL9cfu/mL)；
By above-mentioned bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, lactobacillus acidophilus, brewer's yeast and excrement chain Six plants of bacterial strains of coccus individually ferment, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilis using LBG culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000mL) it cultivates for 24 hours, inoculum concentration 1%, shaking speed 180rpm；
Pediococcus pentosaceus and lactobacillus acidophilus use MRS culture medium (peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000mL) it cultivates for 24 hours, inoculum concentration 1%, shaking speed 180rpm；
Saccharomycete is cultivated using YPD culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000mL) For 24 hours, inoculum concentration 1%, shaking speed 180rpm；
Streptococcus fecalis uses nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000mL) it cultivates for 24 hours, inoculum concentration 1%, shaking speed 180rpm；
(2) the preparation of probiotics composite bacteria liquid
After fermentation to above-mentioned each single strain, by bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, acidophilus Six plants of lactobacillus, brewer's yeast and streptococcus fecalis bacterial strain fresh fermentation broths are mixed by the volume ratio of 1:5:4:6:1:2, are made prebiotic Bacterium composite bacteria liquid, wherein living bacteria count is 1.9 × 1010cfu/mL；
(3) the addition of fresh culture
Added by mass concentration 0.5% into above-mentioned composite bacteria liquid fresh cultured matrix-cane molasses (containing sugar 50%, Brix (Bx) 85%).
(4) protectant addition
Potassium sorbate (purity 98%) is added into liquid obtained in (3) by mass concentration 1 ‰, stirs evenly (120rpm, 1h) obtains pet special bio bacterium taste removal care agent.
Test effect research is as follows:
Test place: Quzhou City, Zhejiang Province Qujiang District Pai Duoge pet shop.
Experimental material: doggie urine, biological bacteria taste removal care agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: experimental setup control group and experimental group are gone by the foul smell of the doggie urine to different disposal form Except the measurement of rate further reflects effectiveness of the invention.
Control group: holding 200mL doggie urine in glass jar, does not add biological bacteria taste removal care agent, and room temperature sealing is placed；
Experimental group: holding 200mL doggie urine in glass jar, in 1 ‰ ratios addition biological bacteria taste removal care agent in urine In, room temperature sealing is placed.
Experimental result: this implementation preparation biological bacteria taste removal care agent handle doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly removed；Ammonia, hydrogen sulfide, methyl mercaptan removal rate measurement result show after for 24 hours: The removal rate of each odorous gas is respectively 70.5%, 75.7% and 80.1%, deodorizing effect highly significant.
Embodiment 2
A kind of production method of pet special bio bacterium taste removal care agent, as shown in Figure 1, including the following steps:
(1): the fermentation of probiotics single strain
The composition of the probiotics single plant bacterial strain is as follows:
Bacillus licheniformis Bacillus Lincheniforms (living bacteria count: 2.0 × 109Cfu/mL), withered grass bud Spore bacillus Bacillus Subtilis (living bacteria count: 6.0 × 109Cfu/mL), Pediococcus pentosaceus Pediococcus Pentosaceus (living bacteria count: 6.0 × 109Cfu/mL), lactobacillus acidophilus Lactobacillus acidophilus (has Effect viable count: 7.0 × 109Cfu/mL), brewer's yeast Sacchaeomyces cerevisiae (living bacteria count: 1.5 × 109) and streptococcus fecalis Streptococcus faecalis (living bacteria count: 2.5 × 10 cfu/mL9cfu/mL)；
By above-mentioned bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, lactobacillus acidophilus, brewer's yeast and excrement chain Six plants of bacterial strains of coccus individually ferment, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilis using LBG culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000mL) culture 30h, inoculum concentration 1.5%, shaking speed 190rpm；
Pediococcus pentosaceus and lactobacillus acidophilus use MRS culture medium (peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000mL) culture 30h, inoculum concentration 1.5%, shaking speed 190rpm；
Saccharomycete is cultivated using YPD culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000mL) 30h, inoculum concentration 1.5%, shaking speed 190rpm；
Streptococcus fecalis uses nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000mL) culture 30h, inoculum concentration 1.5%, shaking speed 190rpm；
(2): the preparation of probiotics composite bacteria liquid
After fermentation to above-mentioned each single strain, by bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, acidophilus Six plants of lactobacillus, brewer's yeast and streptococcus fecalis bacterial strain fresh fermentation broths are mixed by the volume ratio of 2:6:6:7:1.5:2.5, are made Probiotics composite bacteria liquid, wherein living bacteria count is 2.5 × 1010cfu/mL；
(3): the addition of fresh culture
Added by mass concentration 1.0% into above-mentioned composite bacteria liquid fresh cultured matrix-cane molasses (containing sugar 55%, Brix (Bx) 90%).
(4): protectant addition
Potassium sorbate (purity 99%) is added into liquid obtained in (3) by mass concentration 1.2 ‰, stirs evenly (110rpm, 2h) obtains pet special bio bacterium taste removal care agent.
Test effect research is as follows:
Test place: Quzhou City, Zhejiang Province Qujiang District Pai Duoge pet shop.
Experimental material: doggie urine, biological bacteria taste removal care agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: experimental setup control group and experimental group are gone by the foul smell of the doggie urine to different disposal form Except the measurement of rate further reflects effectiveness of the invention.
Control group: holding 200mL doggie urine in glass jar, does not add biological bacteria taste removal care agent, and room temperature sealing is placed；
Experimental group: holding 200mL doggie urine in glass jar, in 1 ‰ ratios addition biological bacteria taste removal care agent in urine In, room temperature sealing is placed.
Experimental result: this implementation preparation biological bacteria taste removal care agent handle doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly removed；Ammonia, hydrogen sulfide, methyl mercaptan removal rate measurement result show after for 24 hours: The removal rate of each odorous gas is respectively 85.1%, 81.3% and 90.1%, deodorizing effect highly significant.
Embodiment 3
A kind of production method of pet special bio bacterium taste removal care agent, as shown in Figure 1, including the following steps:
(1): the fermentation of probiotics single strain
The composition of the probiotics single plant bacterial strain is as follows:
Bacillus licheniformis Bacillus Lincheniforms (living bacteria count: 3.0 × 109Cfu/mL), withered grass bud Spore bacillus Bacillus Subtilis (living bacteria count: 8.0 × 109Cfu/mL), Pediococcus pentosaceus Pediococcus Pentosaceus (living bacteria count: 7.0 × 109Cfu/mL), lactobacillus acidophilus Lactobacillus acidophilus (has Effect viable count: 8.0 × 109Cfu/mL), brewer's yeast Sacchaeomyces cerevisiae (living bacteria count: 2.0 × 109) and streptococcus fecalis Streptococcus faecalis (living bacteria count: 3.0 × 10 cfu/mL9cfu/mL)；
By above-mentioned bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, lactobacillus acidophilus, brewer's yeast and excrement chain Six plants of bacterial strains of coccus individually ferment, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilis using LBG culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000mL) culture 36h, inoculum concentration 2%, shaking speed 200rpm；
Pediococcus pentosaceus and lactobacillus acidophilus use MRS culture medium (peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000mL) culture 36h, inoculum concentration 2%, shaking speed 200rpm；
Saccharomycete is cultivated using YPD culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000mL) 36h, inoculum concentration 2%, shaking speed 200rpm；
Streptococcus fecalis uses nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000mL) culture 36h, inoculum concentration 2%, shaking speed 200rpm；
(2): the preparation of probiotics composite bacteria liquid
After fermentation to above-mentioned each single strain, by bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, acidophilus Six plants of lactobacillus, brewer's yeast and streptococcus fecalis bacterial strain fresh fermentation broths are mixed by the volume ratio of 3:8:7:8:2:3, are made prebiotic Bacterium composite bacteria liquid, wherein living bacteria count is 3.9 × 1010cfu/mL；
(3): the addition of fresh culture
Added by mass concentration 1.5% into above-mentioned composite bacteria liquid fresh cultured matrix-cane molasses (containing sugar 60%, Brix (Bx) 95%).
(4): protectant addition
Potassium sorbate (purity 99%) is added into liquid obtained in (3) by mass concentration 1.5 ‰, stirs evenly (130rpm, 1h) obtains pet special bio bacterium taste removal care agent.
Test effect research is as follows:
Test place: Quzhou City, Zhejiang Province Qujiang District Pai Duoge pet shop.
Experimental material: doggie urine, biological bacteria taste removal care agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: experimental setup control group and experimental group are gone by the foul smell of the doggie urine to different disposal form Except the measurement of rate further reflects effectiveness of the invention.
Control group: holding 200mL doggie urine in glass jar, does not add biological bacteria taste removal care agent, and room temperature sealing is placed；
Experimental group: holding 200mL doggie urine in glass jar, in 1 ‰ ratios addition biological bacteria taste removal care agent in urine In, room temperature sealing is placed.
Experimental result: this implementation preparation biological bacteria taste removal care agent handle doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly removed；Ammonia, hydrogen sulfide, methyl mercaptan removal rate measurement result show after for 24 hours: The removal rate of each odorous gas is respectively 72.1%, 82.3% and 86.1%, deodorizing effect highly significant.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.

Claims (10)

1. a kind of production method of pet special bio bacterium taste removal care agent, which comprises the steps of:
(1): the fermentation of probiotics single strain
The composition of the probiotics single plant bacterial strain and corresponding living bacteria count are as follows:
Bacillus licheniformis, living bacteria count: 1.0 × 109-3.0×109cfu/mL；
Bacillus subtilis, living bacteria count: 5.0 × 109-8.0×109cfu/mL；
Pediococcus pentosaceus, living bacteria count: 4.0 × 109-7.0×109cfu/mL；
Lactobacillus acidophilus, living bacteria count: 6.0 × 109-8.0×109cfu/mL；
Brewer's yeast, living bacteria count: 1.0 × 109-2.0×109cfu/mL；
Streptococcus fecalis, living bacteria count: 2.0 × 109-3.0×109cfu/mL；
By above-mentioned bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, lactobacillus acidophilus, brewer's yeast and streptococcus fecalis Six plants of bacterial strains individually ferment, and obtain corresponding zymocyte liquid；
(2): the preparation of probiotics composite bacteria liquid
After fermentation to above-mentioned each single strain, by bacillus licheniformis, bacillus subtilis, Pediococcus pentosaceus, acidophilus cream bar Six plants of bacterium, brewer's yeast and streptococcus fecalis bacterial strain fresh fermentation broths are mixed according to the volume ratio of 1-3:5-8:4-7:6-8:1-2:2-3 It closes, probiotics composite bacteria liquid is made, wherein living bacteria count is 1.9 × 1010-3.9×1010cfu/mL；
(3): the addition of fresh culture
Culture substrate-cane molasses are added into above-mentioned composite bacteria liquid by mass concentration 0.5%-1.5%；
(4): protectant addition
The potassium sorbate that purity is 98%-100% is added into liquid obtained in (3) by mass concentration 1-1.5 ‰, in 100- In the shaking table of 150rpm revolving speed, uniform stirring 1-2h.
2. a kind of production method of pet special bio bacterium taste removal care agent according to claim 1, which is characterized in that institute State the fermentation of bacillus licheniformis and bacillus subtilis single strain the following steps are included:
The bacillus licheniformis and bacillus subtilis are cultivated into 24-36h, inoculum concentration 1%-2% in LBG culture medium, Shaking speed is 180-200rpm.
3. a kind of production method of pet special bio bacterium taste removal care agent according to claim 2, which is characterized in that institute State the formula of LBG culture medium are as follows: glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, pure water 1000mL.
4. a kind of production method of pet special bio bacterium taste removal care agent according to claim 1, which is characterized in that institute State the fermentation of Pediococcus pentosaceus and lactobacillus acidophilus the following steps are included:
The Pediococcus pentosaceus and lactobacillus acidophilus are cultivated into 24-36h, inoculum concentration 1%-2%, shaking table in MRS culture medium Revolving speed is 180-200rpm.
5. a kind of production method of pet special bio bacterium taste removal care agent according to claim 4, which is characterized in that institute State the formula of MRS culture medium are as follows: peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000mL。
6. a kind of production method of pet special bio bacterium taste removal care agent according to claim 1, which is characterized in that institute State the fermentation of brewer's yeast the following steps are included:
The brewer's yeast is cultivated into 24-36h, inoculum concentration 1%-2%, shaking speed 180- in YPD culture medium 200rpm。
7. a kind of production method of pet special bio bacterium taste removal care agent according to claim 6, which is characterized in that institute State the formula of YPD culture medium are as follows: yeast extract 10g, peptone 20g, glucose 20g, pure water 1000mL.
8. a kind of production method of pet special bio bacterium taste removal care agent according to claim 1, which is characterized in that institute State the fermentation of streptococcus fecalis the following steps are included:
The streptococcus fecalis is cultivated into 24-36h, inoculum concentration 1%-2%, shaking speed 180- in nutrient medium 200rpm。
9. a kind of production method of pet special bio bacterium taste removal care agent according to claim 8, which is characterized in that institute State the formula of nutrient medium are as follows: sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, sodium chloride 2g, sulfuric acid Magnesium 0.2g, pure water 1000mL.
10. a kind of production method of pet special bio bacterium taste removal care agent described in -9 Arbitrary Terms according to claim 1, special Sign is that the sugar content of the fresh cultured matrix-cane molasses is 50%-100%, and brix (Bx) is 85%-100%.
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