CN105624072A - Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof - Google Patents
Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an active microbial agent for deodorizing livestock and poultry feces. The active microbial agent comprises six types of strains, namely bacillus subtilis, bacillus cereus, arthrobacterium, streptomycete, saccharomycetes and lactic streptococci. The invention further discloses a preparation method of the active microbial agent for deodorizing livestock and poultry feces. The active microbial agent for deodorizing livestock and poultry feces and the preparation method thereof utilize synergism of the six types of feces degrading bacteria or deodorization bacteria and have the advantages of being novel in strain combination, stable in structure, great in enzyme activity, large in bacteria number and the like, meanwhile, the problems that in traditional compost treatment of livestock and poultry feces, feces-urine separation is required, feces degradation is low in speed, and the deodorizing effect is not obvious are solved, and the livestock and poultry feces treatment speed and utilization rate are raised.
Description
Technical field
The present invention relates to ecologic breeding field, it is specifically related to deodorant activities bacterium agent and its preparation method of a kind of fowl and animal excrement the like waste.
Background technology
Along with the development of China's livestock and poultry breeding industry and the enforcement of " vegetable basket project ", mass-producing, intensive livestock and poultry breeding industry emerge rapidly, and the generation of feces of livestock and poultry increases year by year. Just the fowl and animal excrement of discharge contains NH3, the obnoxious flavour such as H2S and indoles, if can not process in time after failing to remove in time or remove, stink can be multiplied, and produces that methyl sulphur is liquor-saturated, the foul gas such as dimethyl disulfide, dimethyl sulfide, dimethylamine and lower fatty acid. Measuring and calculating shows, the pig farm ammonia every day generation delivering 5000 year for sale reaches more than 0.8Kg. Foul gas to healthy generation detrimentally affect that is on-the-spot and people around, as caused lassitude, agitation, hypomnesis and psychologic status bad, also can make disease resistance and the reducing of the productivity of poultry. Therefore, process fowl and animal excrement in time, remove stench and obnoxious flavour that it produces, be one of the major issue needing solution badly of China's development green cultivation, ecologic breeding.
The treatment process of fowl and animal excrement is a lot, is separated mainly through excrement urine at present and processes respectively. Anaerobic methane production method, anaerobic digestion method, contact oxidation method, sequencing batch active sludge, Photosynthetic Bacteria, artificial swamp method etc. are mainly contained for the process of liquid several, desiccating method, chemical method, biological composting process etc. are then mainly contained for ight soil solid. In various treatment process, although desiccating method and chemical method processing speed are fast, but the cost of required energy consumption or medicine is all higher. Although biological composting process cost is lower, but needs Special Equipment, sewage and ight soil solid process respectively, and operate complex steps. Therefore, research and development do not need Special Equipment to carry out excrement urine separation, can Direct Resolution fowl and animal excrement and sewage, the deodorizing microorganism of removing stench and deodorization process under aerobic condition, for solution plant stink to the harm of human body and environment, improve process utilization ratio and the economic worth of fowl and animal excrement, significant.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is provided that the deodorant activities bacterium agent of a kind of fowl and animal excrement the like waste and its preparation method.
The present invention adopts following technique means to solve the problems of the technologies described above: a kind of fowl and animal excrement deodorant activities bacterium agent, comprises Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36, yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium.
The present invention also discloses a kind of method preparing above-mentioned fowl and animal excrement deodorant activities bacterium agent, comprises the steps:
1) single strain fermentation: Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36, yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium are fermented respectively, obtains zymocyte liquid;
2) deodorizing composite fungus agent preparation: after above-mentioned each single strain fermentation ends, by subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria mixing, obtained deodorizing composite fungus agent;
3) fresh culture adds: add fresh culture matrix in composite fungus agent;
4) protective material adds: to step 3) in the liquid that obtains adds potassium sorbate, stir evenly;
5) carrier adds: to step 4) in the liquid that obtains adds attapulgite, stir evenly, i.e. obtained fowl and animal excrement deodorant activities bacterium agent.
Preferably, subtilis B.subtilisCZ9 and bacillus cereus B.cereusSZ2 adopts LBG culture medium culturing; Described LBG substratum comprises 0.5% glucose, 1% peptone, 0.5% yeast extract paste, 1% sodium-chlor; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
Preferably, streptomycete Streptomycessp.SZ36 adopts SCP culture medium culturing; Described SCP substratum comprises 1.0% sucrose, 2.0% Semen Maydis powder, peptone 0.3%, soybean cake powder 3.0%, 0.1% potassium primary phosphate, ammonium sulfate 0.5%, 0.1% sodium-chlor, 0.3% calcium carbonate; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
Preferably, Arthrobacter Arthrobactersp.SZ16 adopts LB culture medium culturing; Described LB substratum comprises 1% Tryptones, 0.5% yeast extract paste, 1% sodium-chlor; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
Preferably, yeast S.roseusCZ13 adopts YPD culture medium culturing; Described YPD substratum comprises 1% yeast extract, 2% peptone, 2% D-glucose; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
Preferably, streptococcus acidi lactici S.lactisCZ15 adopts fermention medium to cultivate, and described fermention medium comprises sucrose 1%, peptone 2%, yeast extract paste 1%, KH2PO43%, NaCl0.2%, MgSO40.02%, inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
Preferably, after above-mentioned each single strain fermentation ends, subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria are mixed according to the volume ratio of 2-5:0.5-2:1-2:1-3:0.5-3:3-8, obtained deodorizing composite fungus agent; The living bacteria count of obtained composite fungus agent is 8.0 �� 108-2.3��109cfu/ml��
Preferably, in composite fungus agent, fresh culture matrix-cane molasses is added by mass concentration 2%-5%; Described molasses contain sugar more than 50%, brix (Bx) more than 85%.
Preferably, in mass concentration 1-1.5 �� to 3) in the liquid that obtains adds the potassium sorbate of 1-1.5 �� ratio, mixing parametric is: 120rpm, stirs 1h; To 4) add attapulgite amount be 0.4-1.0g/L, mixing parametric: 40-100rpm, stir 4-10min.
It is an advantage of the current invention that: fowl and animal excrement deodorant activities bacterium agent provided by the invention utilizes the synergy between six kinds of dejection degradation bacterium/deodorizing bacterium, there is the features such as strain combinations is new, structure is steady, enzyme is lived by force, bacterium number height, solve the problems such as tradition fowl and animal excrement compost treatment needs excrement urine separation, dejection degradation is slow, deodorizing effect is not obvious simultaneously, improve processing speed and the utilization ratio of fowl and animal excrement, ight soil through deodorizing process also can do fertilizer and be administered in farmland, increase soil fertility, there is good ecological benefits and economic benefit. Its technique of composite fungus agent provided by the invention is simple, and the protective material adopted in preparation process is all non-toxic substance, can ensure the safety of bacterium agent.
Embodiment
Following examples illustrate the content of the present invention further, but should not be construed as limitation of the present invention. Without departing from the spirit and substance of the case in the present invention, the amendment do the inventive method, step or condition or replacement, all belong to the scope of the present invention.
Embodiment 1
The formation of deodorant activities bacterium agent
Subtilis B.subtilisCZ9,2.0 �� 108Cfu/ml;
Bacillus cereus B.cereusSZ2,5.0 �� 107cfu/ml
Arthrobacter Arthrobactersp.SZ16,1.0 �� 108cfu/ml
Streptomycete Streptomycessp.SZ36,1.0 �� 108cfu/ml��
Yeast S.roseusCZ13,5.0 �� 107cfu/ml��
Streptococcus acidi lactici S.lactisCZ15,3.0 �� 108cfu/ml��
Constituted mode bacterium agent as above is obtained by following step:
1) single strain fermentation: Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36 and yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium are fermented respectively, obtains zymocyte liquid.
Subtilis B.subtilisCZ9 and bacillus cereus B.cereusSZ2 adopts LBG substratum (0.5% glucose, 1% peptone, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate; 1% inoculum size inoculation, 180rpm, cultivates 24h.
Streptomycete Streptomycessp.SZ36 adopts SCP substratum (1.0% sucrose, 2.0% Semen Maydis powder, peptone 0.3%, soybean cake powder 3.0%, 0.1% potassium primary phosphate, ammonium sulfate 0.5%, 0.1% sodium-chlor, 0.3% calcium carbonate) to cultivate; 1% inoculum size inoculation, 180rpm, cultivates 24h.
Arthrobacter Arthrobactersp.SZ16 adopts LB substratum (1% Tryptones, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate. Respectively with 1% inoculum size inoculation, 180rpm, cultivates 24h.
Yeast S.roseusCZ13 adopts YPD substratum (1% yeast extract, 2% peptone, 2% D-glucose) to cultivate; 1% inoculum size inoculation, 180rpm, cultivates 24h.
Streptococcus acidi lactici S.lactisCZ15 adopts fermention medium (1% sucrose, 2% peptone, 1% yeast extract paste, 3%KH2PO4, 0.2%NaCl, 0.02%MgSO4) cultivate. 1% inoculum size inoculation, 180rpm, cultivates 24h. In the embodiment of the present invention, other compositions of all substratum are pure water.
2) deodorizing composite fungus agent preparation: after above-mentioned each single strain fermentation ends, subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria are mixed according to the volume ratio of 2:0.5:1:1:0.5:3, obtained deodorizing composite fungus agent. The living bacteria count of obtained composite fungus agent is 8.0 �� 108cfu/ml��
3) fresh culture adds: the fresh culture matrix-cane molasses (containing sugar more than 50%, brix (Bx) more than 85%) added in composite fungus agent by mass concentration 2%.
4) protective material adds: by mass concentration 1 �� to 3) in the liquid that obtains adds potassium sorbate (purity more than 98%), stir evenly (120rpm stirs 1h).
5) carrier adds: to 4) in the liquid that obtains adds attapulgite (0.4g/L), and stir evenly (40rpm, 4 minutes), namely obtain fowl and animal excrement deodorant activities bacterium agent.
The deodorizing microorganism process swine excrement test effect research that in pig farm prepared by Application Example 1
Test site: glad auspicious pig-breeding field, Qujiang District, Quzhou City of Zhejiang Province
Test materials: swine excrement, fowl and animal excrement deodorant activities bacterium agent prepared by embodiment 1.
Test design: arranging two process, each septic tank treatment capacity is 100 tons.
Process 1: not adding fowl and animal excrement deodorant activities bacterium agent in septic tank, fowl and animal excrement directly enters in septic tank.
Process 2: after fowl and animal excrement enters septic tank, add this deodorant activities bacterium agent by 1 �� volume ratios in septic tank.
Application process: after being added in septic tank by fowl and animal excrement deodorant activities bacterium agent, adopts portable gas blower oxygen supply, oxygenation every day 1h, oxygenation capacity 200m3, all the other time leaves standstill, and a point Different periods measures the removal effect to ight soil stink respectively.
Test-results: using fowl and animal excrement deodorant activities bacterium agent process ight soil prepared by embodiment 1,24h stink obviously declines, and after 48h, ammonia, hydrogen sulfide, thiomethyl alcohol clearance are respectively 70.5,75.7% and 80.1%, and deodorizing effect is very remarkable.
Embodiment 2
The formation of deodorant activities bacterium agent
Subtilis B.subtilisCZ9,3.0 �� 108Cfu/ml;
Bacillus cereus B.cereusSZ2,1.0 �� 108cfu/ml
Arthrobacter Arthrobactersp.SZ16,1.5 �� 108cfu/ml
Streptomycete Streptomycessp.SZ36,2.0 �� 108cfu/ml��
Yeast S.roseusCZ13,1.5 �� 108cfu/ml��
Streptococcus acidi lactici S.lactisCZ15,5.0 �� 108cfu/ml��
1) single strain fermentation: Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36 and yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium are fermented respectively, obtains zymocyte liquid.
Subtilis B.subtilisCZ9 and bacillus cereus B.cereusSZ2 adopts LBG substratum (0.5% glucose, 1% peptone, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate; 1.5% inoculum size, 190rpm, cultivates 30h.
Streptomycete Streptomycessp.SZ36 adopts SCP substratum (1.0% sucrose, 2.0% Semen Maydis powder, peptone 0.3%, soybean cake powder 3.0%, 0.1% potassium primary phosphate, ammonium sulfate 0.5%, 0.1% sodium-chlor, 0.3% calcium carbonate) to cultivate; 1.5% inoculum size inoculation, 190rpm, cultivates 30h.
Arthrobacter Arthrobactersp.SZ16 adopts LB substratum (1% Tryptones, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate. 1.5% inoculum size inoculation, 190rpm, cultivates 30h.
Yeast S.roseusCZ13 adopts YPD substratum (1% yeast extract, 2% peptone, 2% D-glucose) to cultivate; 1.5% inoculum size inoculation, 190rpm, cultivates 30h.
Streptococcus acidi lactici S.lactisCZ15 adopts fermention medium (1% sucrose, 2% peptone, 1% yeast extract paste, 3%KH2PO4, 0.2%NaCl, 0.02%MgSO4) cultivate. 1.5% inoculum size inoculation, 190rpm, cultivates 30h.
2) deodorizing composite fungus agent preparation: after above-mentioned each single strain fermentation ends, subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria are mixed according to the volume ratio of 3:1:1.5:2:1.5:5, obtained deodorizing composite fungus agent. The living bacteria count of obtained composite fungus agent is 1.4 �� 109cfu/ml��
3) fresh culture adds: the fresh culture matrix-cane molasses (containing sugar more than 50%, brix (Bx) more than 85%) added in composite fungus agent by mass concentration 3%.
4) protective material adds: by mass concentration 1.3 �� to 3) in the liquid that obtains adds potassium sorbate (purity more than 98%), stir evenly (120rpm stirs 1h).
5) carrier adds: to 4) in the liquid that obtains adds attapulgite (0.6g/L), and stir evenly (60rpm, 6 minutes), namely obtain fowl and animal excrement deodorant activities bacterium agent.
The deodorizing microorganism process swine excrement test effect research that in pig farm prepared by Application Example 2
Test site: glad auspicious pig-breeding field, Qujiang District, Quzhou City of Zhejiang Province
Test materials: swine excrement, fowl and animal excrement deodorant activities bacterium agent prepared by embodiment 2.
Test design: arranging two process, each septic tank treatment capacity is 100 tons.
Process 1: not adding fowl and animal excrement deodorant activities bacterium agent in septic tank, fowl and animal excrement directly enters in septic tank.
Process 2: after fowl and animal excrement enters septic tank, adds in 1 �� ratios in septic tank and uses this fowl and animal excrement deodorant activities bacterium agent.
Application process: after being added in septic tank by fowl and animal excrement deodorant activities bacterium agent, adopts portable gas blower oxygen supply, oxygenation every day 1h, oxygenation capacity 200m3, all the other time leaves standstill, and a point Different periods measures the removal effect to ight soil stink respectively.
Test-results: using fowl and animal excrement deodorant activities bacterium agent process ight soil prepared by embodiment 2,24h stink obviously declines, and after 48h, ammonia, hydrogen sulfide, thiomethyl alcohol clearance are respectively 89.5,91.7% and 92.1%, and deodorizing effect is very remarkable.
Embodiment 3
The formation of deodorant activities bacterium agent
Subtilis B.subtilisCZ9,5.0 �� 108Cfu/ml;
Bacillus cereus B.cereusSZ2,2.0 �� 108cfu/ml
Arthrobacter Arthrobactersp.SZ16,2.0 �� 108cfu/ml
Streptomycete Streptomycessp.SZ36,3.0 �� 108cfu/ml��
Yeast S.roseusCZ13,3.0 �� 108cfu/ml��
Streptococcus acidi lactici S.lactisCZ15,8.0 �� 108cfu/ml��
1) single strain fermentation: Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36 and yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium are fermented respectively, obtains zymocyte liquid.
Subtilis B.subtilisCZ9 and bacillus cereus B.cereusSZ2 adopts LBG substratum (0.5% glucose, 1% peptone, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate; 2.0% inoculum size inoculation, 200rpm, cultivates 36h.
Streptomycete Streptomycessp.SZ36 adopts SCP substratum (1.0% sucrose, 2.0% Semen Maydis powder, peptone 0.3%, soybean cake powder 3.0%, 0.1% potassium primary phosphate, ammonium sulfate 0.5%, 0.1% sodium-chlor, 0.3% calcium carbonate) to cultivate; 2.0% inoculum size inoculation, 200rpm, cultivates 36h.
Arthrobacter Arthrobactersp.SZ16 adopts LB substratum (1% Tryptones, 0.5% yeast extract paste, 1% sodium-chlor) to cultivate. Respectively with 2.0% inoculum size inoculation, 200rpm, cultivates 36h.
Yeast S.roseusCZ13 adopts YPD substratum (1% yeast extract, 2% peptone, 2% D-glucose) to cultivate; 2.0% inoculum size inoculation, 200rpm, cultivates 36h.
Streptococcus acidi lactici S.lactisCZ15 adopts fermention medium (1% sucrose, 2% peptone, 1% yeast extract paste, 3%KH2PO4, 0.2%NaCl, 0.02%MgSO4) cultivate. 2.0% inoculum size inoculation, 200rpm, cultivates 36h.
2) deodorizing composite fungus agent preparation: after above-mentioned each single strain fermentation ends, subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria are mixed according to the volume ratio of 5:2:2:3:3:8, obtained deodorizing composite fungus agent. The living bacteria count of obtained composite fungus agent is 2.3 �� 109cfu/ml��
3) fresh culture adds: the fresh culture matrix-cane molasses (containing sugar more than 50%, brix (Bx) more than 85%) added in composite fungus agent by mass concentration 5%.
4) protective material adds: by mass concentration 1.5 �� to 3) in the liquid that obtains adds potassium sorbate (purity more than 98%), stir evenly (120rpm stirs 1h).
5) carrier adds: to 4) in the liquid that obtains adds attapulgite (1.0g/L), and stir evenly (100rpm, 10 minutes), namely obtain fowl and animal excrement deodorant activities bacterium agent.
The deodorizing microorganism process swine excrement test effect research that in pig farm prepared by Application Example 3
Test site: glad auspicious pig-breeding field, Qujiang District, Quzhou City of Zhejiang Province
Test materials: swine excrement, fowl and animal excrement deodorant activities bacterium agent prepared by embodiment 3.
Test design: arranging two process, each septic tank treatment capacity is 100 tons.
Process 1: not adding fowl and animal excrement deodorant activities bacterium agent in septic tank, fowl and animal excrement directly enters in septic tank.
Process 2: after fowl and animal excrement enters septic tank, add this fowl and animal excrement deodorant activities bacterium agent in 1 �� ratios in septic tank.
Application process: after being added in septic tank by fowl and animal excrement deodorant activities bacterium agent, adopts portable gas blower oxygen supply, oxygenation every day 1h, oxygenation capacity 200m3, all the other time leaves standstill, and a point Different periods measures the removal effect to ight soil stink respectively.
Test-results: using fowl and animal excrement deodorant activities bacterium agent process ight soil prepared by embodiment 3,24h stink obviously declines, and after 48h, ammonia, hydrogen sulfide, thiomethyl alcohol clearance are respectively 88.1,86.3% and 80.5%, and deodorizing effect is very remarkable.
Claims (10)
1. a fowl and animal excrement deodorant activities bacterium agent, it is characterised in that: comprise Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36, yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium.
2. prepare the method for fowl and animal excrement deodorant activities bacterium agent as claimed in claim 1 for one kind, it is characterised in that, comprise the steps:
1) single strain fermentation: Bacillus subtilis CZ9, bacillus cereus BacilluscereusSZ2, Arthrobacter Arthrobactersp.SZ16, streptomycete Streptomycessp.SZ36, yeast SporobolomycesroseusCZ13, streptococcus acidi lactici StreptococcuslactisCZ15 six strain bacterium are fermented respectively, obtains zymocyte liquid;
2) deodorizing composite fungus agent preparation: after above-mentioned each single strain fermentation ends, by subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria mixing, obtained deodorizing composite fungus agent;
3) fresh culture adds: add fresh culture matrix in composite fungus agent;
4) protective material adds: to step 3) in the liquid that obtains adds potassium sorbate, stir evenly;
5) carrier adds: to step 4) in the liquid that obtains adds attapulgite, stir evenly, i.e. obtained fowl and animal excrement deodorant activities bacterium agent.
3. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, subtilis B.subtilisCZ9 and bacillus cereus B.cereusSZ2 adopts LBG culture medium culturing; Described LBG substratum comprises 0.5% glucose, 1% peptone, 0.5% yeast extract paste, 1% sodium-chlor; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
4. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, streptomycete Streptomycessp.SZ36 adopts SCP culture medium culturing; Described SCP substratum comprises 1.0% sucrose, 2.0% Semen Maydis powder, peptone 0.3%, soybean cake powder 3.0%, 0.1% potassium primary phosphate, ammonium sulfate 0.5%, 0.1% sodium-chlor, 0.3% calcium carbonate; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
5. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, Arthrobacter Arthrobactersp.SZ16 adopts LB culture medium culturing; Described LB substratum comprises 1% Tryptones, 0.5% yeast extract paste, 1% sodium-chlor; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
6. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, yeast S.roseusCZ13 adopts YPD culture medium culturing; Described YPD substratum comprises 1% yeast extract, 2% peptone, 2% D-glucose; Inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
7. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, streptococcus acidi lactici S.lactisCZ15 adopts fermention medium to cultivate, and described fermention medium comprises sucrose 1%, peptone 2%, yeast extract paste 1%, KH2PO43%, NaCl0.2%, MgSO40.02%, inoculum size is 1%-2%, 180-200rpm, cultivates 24-36h.
8. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterized in that, after above-mentioned each single strain fermentation ends, subtilis, bacillus cereus, Arthrobacter, streptomycete, yeast, milk-acid bacteria are mixed according to the volume ratio of 2-5:0.5-2:1-2:1-3:0.5-3:3-8, obtained deodorizing composite fungus agent; The living bacteria count of obtained composite fungus agent is 8.0 �� 108-2.3��109cfu/ml��
9. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterised in that, in composite fungus agent, add fresh culture matrix-cane molasses by mass concentration 2%-5%; Described molasses contain sugar more than 50%, brix (Bx) more than 85%.
10. a kind of method preparing fowl and animal excrement deodorant activities bacterium agent according to claim 2, it is characterized in that, in mass concentration 1-1.5 �� to 3) in the liquid that obtains adds the potassium sorbate of 1-1.5 �� ratio, mixing parametric is: 120rpm, stirs 1h; To 4) add attapulgite amount be 0.4-1.0g/L, mixing parametric: 40-100rpm, stir 4-10min.
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| CN106187331A (en) * | 2016-07-13 | 2016-12-07 | 中国科学院合肥物质科学研究院 | One utilizes fowl and animal excrement to prepare complex microorganism ecology bacterial manure and method thereof |
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| CN106673385A (en) * | 2017-02-09 | 2017-05-17 | 合肥隆扬农业科技有限公司 | Microbial deodorizing liquid for pig manure and preparation method of microbial deodorizing liquid |
| CN106883037A (en) * | 2017-03-23 | 2017-06-23 | 贵州大学 | Duck excrement deodorant and the method that duck excrement biological organic fertilizer is prepared using the deodorant |
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| CN106187331B (en) * | 2016-07-13 | 2019-11-05 | 中国科学院合肥物质科学研究院 | It is a kind of to prepare complex microorganism ecology bacterial manure and its method using fowl and animal excrement |
| CN106361683A (en) * | 2016-08-29 | 2017-02-01 | 中国科学院合肥物质科学研究院 | Preparation method of special biological microbe smell removal care agent for pets |
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| CN106883037A (en) * | 2017-03-23 | 2017-06-23 | 贵州大学 | Duck excrement deodorant and the method that duck excrement biological organic fertilizer is prepared using the deodorant |
| CN107324508B (en) * | 2017-07-05 | 2019-02-22 | 季思成 | A method of based on special efficacy microorganism fast degradation is used to dissolve solid-state fecal pollution object |
| CN107324508A (en) * | 2017-07-05 | 2017-11-07 | 季思成 | It is a kind of based on using special efficacy microorganism fast degradation so as to the method for solid-state fecal pollution thing of dissolving |
| CN107261818A (en) * | 2017-07-25 | 2017-10-20 | 哈尔滨天储生物科技有限公司 | A kind of liquid fowl and animal excrement deodorization fly eradication liquid and preparation method |
| CN108300668A (en) * | 2018-01-26 | 2018-07-20 | 重庆鹓雏农业科技有限公司 | Organic matter speciality fermenting agent |
| CN108300668B (en) * | 2018-01-26 | 2021-08-17 | 重庆鹓雏农业科技有限公司 | Organic matter special fermentation bacterial agent |
| CN108913626A (en) * | 2018-07-26 | 2018-11-30 | 中国科学院合肥物质科学研究院 | A kind of fowl and animal excrement deodorization composite bacteria agent and preparation method thereof |
| CN108913626B (en) * | 2018-07-26 | 2022-02-11 | 中国科学院合肥物质科学研究院 | Livestock and poultry manure deodorizing composite microbial inoculum and preparation method thereof |
| CN110042071A (en) * | 2019-04-02 | 2019-07-23 | 辽宁石油化工大学 | A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof |
| CN110463662A (en) * | 2019-09-27 | 2019-11-19 | 台圣环保设备(马鞍山)有限公司 | A kind of live stock feeding method |
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