CN107801837A - A kind of fodder utilizes the bio-fermentation process of chicken manure - Google Patents
A kind of fodder utilizes the bio-fermentation process of chicken manure Download PDFInfo
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- CN107801837A CN107801837A CN201711410167.5A CN201711410167A CN107801837A CN 107801837 A CN107801837 A CN 107801837A CN 201711410167 A CN201711410167 A CN 201711410167A CN 107801837 A CN107801837 A CN 107801837A
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- 210000003608 fece Anatomy 0.000 title claims abstract description 95
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 60
- 239000010871 livestock manure Substances 0.000 title claims abstract description 58
- 241000894006 Bacteria Species 0.000 claims abstract description 82
- 230000004151 fermentation Effects 0.000 claims abstract description 81
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 35
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 31
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 22
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 22
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 16
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 16
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- 238000005516 engineering process Methods 0.000 claims abstract description 16
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- 241001465754 Metazoa Species 0.000 description 8
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- 229920000742 Cotton Polymers 0.000 description 1
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000002479 acid--base titration Methods 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
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- 238000000556 factor analysis Methods 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 239000002515 guano Substances 0.000 description 1
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- 239000010842 industrial wastewater Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses the bio-fermentation process that a kind of fodder utilizes chicken manure, integrated modern mixed bacteria microorganism fermentation technique, develop high protein, high amino acid content, low content of cellulose, the chicken manure protein feed of high price of deed rate, the technology zymotechnique is simple efficiently, deodorizing sterilizing effect is good, production cost is low, is easy to industrial-scale production.The application takes mixed bacterium aerobic fermentation and mixed bacterium anaerobic fermentation two-step fermentation technique, and wherein aerobic fermentation flora includes mould, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium；Anaerobic fermentation flora includes one or more of mixtures in streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus.The technique uses more bacterium mixed fungus fermentations, by the combination of different metabolic ability, the complicated metabolism that single strain is difficult to complete is completed, passes through effective conversion of the cooperative fermentation between microorganism to chicken manure, sanitary index is up to state standards requirement, and product nutritive value index is obviously improved.
Description
Technical field
It is especially a kind of to be assisted using a variety of probiotics the present invention relates to the bio-fermentation process that a kind of fodder utilizes chicken manure
The bio-fermentation process of high protein content, high price of deed rate fermentation poultry dung feed is prepared with fermentation, belongs to bioengineering field.
Background technology
With the development of Modern Animal Husbandry, poultry farms scale, intensive degree improve constantly, large-scale poultry farming
Excrement caused by seriously pollutes environment.State Environmental Protection Administration shows for 2002 to scale animal and poultry cultivation Survey of contamination status of sea products result
Show：Chinese festiva feces of livestock and poultry amount is about 1,900,000,000 t, wherein, COD（COD）Discharge capacity oneself up to 71,180,000 t, feces of livestock and poultry production
Amount and COD discharge capacitys are all considerably beyond industrial wastewater and discharging life water amount sum, and feces of livestock and poultry total amount in 2004 is 28
Hundred million t；Reach 4,500,000,000 t within 2010.This is more and more serious to the mankind, other biological and poultry its own existence environment pollutions.From
From the point of view of environment, it causes serious pollution to air, waters and soil, deteriorates ball ecological environment.From resource
Angle sees that it is good renewable resource.If being translated into feed, self recycling for Animal Husbandry Resources is realized,
Can not only solve the shortage of resources of current facing mankind and the hang-up of environmental pollution two, and grain is striven for alleviating people and animals
Contradiction, the Recycling economy of animal husbandry of sustainable development is realized, all tool is of great significance.
The alimentary canal of chicken is short, and the feed residence time is not grown, and its excrement gross protein value is high, and rich in several amino acids,
Calcium, phosphorus and vitamin etc., there is result of study to show in chicken manure dry, crude protein 28.0%, pure protein 11.3%, egg can be digested
White matter 14.4%, crude fat 2% or so, crude fibre 10% or so, calcium 6%-8%, phosphorus 2.5%-3.0%, also containing zinc, copper,
The mineral elements such as iron and abundant B family vitamin.Therefore, it is a kind of compare to be changed into chicken manure using feed by processing
Preferable Harmless processing mode.
Poultry manure technology in terms of Fertilizer Transformed and recycling is ripe day by day at present, but in fodder processing side
The drawbacks of more also be present in face.In terms of Fodder making, treatment effeciency highest method mainly have chemical method, physical treatment process and
Thermal jet regenerative chicken feces feed method etc., not only cost is higher for these methods, the nutriment that can also destroy in material, reduces economy
Effect is overflow.Beneficial microbe has that breeding is fast, metabolism is prosperous, and fermented feed can detoxify, detoxification, and can accumulate useful intermediate supersession and produce
Thing, its palatability is improved, using microbial fermentation substantial amounts of agricultural byproducts and discarded object can be made to change into feed, thus in biology
In terms of method prepares feed, also there are many people studying, they add the microorganisms such as EM bacterium in material, and are additionally added corn
The conditioner such as powder and wheat bran, then carries out silage fermentation, can substantially shorten fermentation time, and can also improve the product of feed
Matter, as patent CN201510642108.5 discloses a kind of pig feed containing chicken manure；Patent CN201510703677.6 is disclosed
A kind of poultry dung feed and preparation method thereof；Patent CN200710115062.7 discloses a kind of animal wastes（Containing chicken manure）For original
Microbiological feed of material and preparation method thereof；Patent CN201611037162.8 disclose it is a kind of strengthen pig immunity feed and
Its preparation method；Patent CN201410514712.5 discloses a kind of method of recycling brid guano production swine feed.
Although biological fermentation process prepares the technique of poultry dung feed and reached its maturity at present, still suffered from current technique complex manufacturing,
Production cost is high, fodder still needs to the further drawback such as lifting using horizontal low, palatability.
The content of the invention
In order to overcome the above-mentioned deficiencies of the prior art, meet chicken manure harmless treatment and market to high protein, high feed report
The demand of reward rate poultry dung feed, the present invention provide the bio-fermentation process that a kind of fodder utilizes chicken manure, and it is micro- to integrate modern mixed bacterium
Biofermentation technique, develop high protein, high amino acid content, low content of cellulose, the chicken manure egg of high price of deed rate
White feed, the technology zymotechnique is simple efficiently, deodorizing sterilizing effect is good, and production cost is low, is easy to industrially scalable life
Production.
The present invention is achieved through the following technical solutions above-mentioned technique effect：
A kind of fodder utilizes the bio-fermentation process of chicken manure, specifically includes mixed bacterium aerobic fermentation and the mixed step of bacterium anaerobic fermentation two hair
Ferment, wherein aerobic fermentation flora include mould, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium；Anaerobic fermentation bacterium
Group includes one or more of mixtures in streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus.
Mould, saccharomycete, bacillus subtilis, the amount ratio of thermophilic heterotrophic ammonia-oxidizing bacterium in above-mentioned aerobic fermentation flora
For 1.2:1.8:0.6:1.
Above-mentioned mould is aspergillus niger, one or two kinds of Mixed Microbes of sporotrichum thermophile bacterium；
Above-mentioned saccharomycete can be mixing one or more of in candida utili, brewer's yeast, saccharomyces cerevisiae, molasses yeast
Bacterium；
Preferably, the optimal strain of saccharomycete is the Mixed Microbes of candida utili and molasses yeast, and its amount ratio is 1:0.75.
Preferably, the optimal flora of anaerobic fermentation flora is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus inoculation
Ratio 1:1:3 mixed bacterial.
The preparation method of the seed liquor of above-mentioned aspergillus niger or sporotrichum thermophile bacterium is：Aseptically, trained using potato
Support base and cultivate 24h under the conditions of 35~40 DEG C, aspergillus niger or sporotrichum thermophile bacterium seed liquor is made, it is desirable to effective work of seed liquor
Bacterium number >=109cfu/mL。
Above-mentioned saccharomycete seed liquor preparation method is：Under aseptic technique, oese is used from saccharomycete YDP inclined-planes
The ring strain of picking two, it is inoculated into the triangular flask of 500mL dress 300mLPDA fluid nutrient mediums, 28 DEG C~32 DEG C, 140r/min shakes
After swinging culture 24h, then expand culture according to above-mentioned technique inoculation seeding tank and prepare saccharomycete seed liquor, it is desirable to which seed liquor has
Imitate viable count >=109cfu/mL。
Above-mentioned bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquid and preparation method thereof are：Aseptically, use
LB culture mediums cultivate 24h under the conditions of 28~32 DEG C, liquid bacillus seed liquor are made, it is desirable to the living bacteria count of seed liquor
≥109cfu/mL。
Above-mentioned streptococcus thermophilus, urine enterococcus and the seed of lactobacillus acidophilus liquid and preparation method thereof are：By inclined-plane or glycerol tube
Preservation of bacteria strain is seeded to LB culture mediums by corresponding seed culture medium, wherein clostridium butyricum, and lactic acid bacteria culturers connect
For kind into MRS culture mediums, 37 DEG C of 20~30h of quiescent culture obtain primary seed solution；Then primary seed solution is inoculated into 10L's
In vial seed fermentation tank, 37 DEG C of 20~30h of fermentation obtain secondary seed solution, it is desirable to and the living bacteria count of seed liquor >=
109cfu/mL。
Present invention also offers the bio-fermentation process that a kind of fodder utilizes chicken manure, its concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is 3~5wt% of solid medium, mixes rear compost fermentation thoroughly,
24~30h of aerlbic culture；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.5~2wt%, 12~16h of anaerobic fermentation；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
The chicken manure of the preferred chick of chicken manure in above-mentioned technique.
The invention provides the bio-fermentation process that a kind of fodder utilizes chicken manure, gained fermentation poultry dung stink all disappears
Lose, quality is soft, not agglomerating, with denseer sour fragrance；Rich in probiotics, vitamin, enzyme and amino acid, short in product
The nutritional ingredients such as peptide, good protein, product nutrition, safety, green, there is enhancing development, improve efficiency of feed utilization, increase
Strong immunity, the advantages that improving intestinal microecology, preventing disease, reduce environmental pollution.The product can be directly used for feeding pig, chicken
Deng animal, can also be fed with coordinating after feed collocation.
To verify the wholesomeness of product of the present invention, its sanitary index is determined, and is contrasted with national standard, it is as a result as follows
Shown in table.Measurement result shows that the application product meets state health standards.
The application product sanitary index of table 1
The present invention provides the bio-fermentation process that a kind of fodder utilizes chicken manure, compared with prior art, has following significantly excellent
Gesture：
（1）The application utilizes aerobic bacteria（Aspergillus niger, sporotrichum thermophile bacterium, saccharomycete, bacillus subtilis, thermophilic heterotrophism ammonia oxygen
Change bacterium）And anaerobic bacteria（Streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus）Co-fermentation fodder comprehensively utilizes chicken manure, fills
Point using microbial bacteria inter-species synergy, eliminate restrict poultry dung feedization utilize existing for stink is big, a large amount of cause a disease be present
Bacterium, using the drawback such as albumen is relatively low, the palatability and trophic level of fermentation poultry dung are lifted, so as to be obviously improved the feeding of cotton dregs
Nutritive value, wherein：The ability of aspergillus niger assimilation starch and cellulose is strong, can be degraded to the structural carbohydrate of chicken manure
The monosaccharide materials such as the available monosaccharide and disaccharide of yeast, make yeast, bacillus, lactic acid bacteria etc. be able to good growth；Together
Sample, sporotrichum thermophile bacterium the synergy between decomposition of cellulose, with aspergillus niger can obtain " 1+1 at high temperature>2 " technology
Effect；The presence of saccharomycete can be by quickly breeding the mycoprotein of increase product, and can suppress the albumen quilt in chicken manure
Decompose, avoid the occurrence of the phenomenon for occurring crude protein decline in fermentation process；Meanwhile the quick breeding of saccharomycete makes under material pH value
Drop, suppress the growth for including the pathogenic bacteria such as salmonella；Bacillus subtilis can be killed during hot fermentation in chicken manure
Pathogenic bacteria, and there is stronger production protease ability, can be that cultivated animals are more easy to digest by high molecular weight protein mass degradation
Small molecular weight protein, peptide and the amino acid of absorption；The presence of thermophilic heterotrophic ammonia-oxidizing bacterium can prevent and treat the generation of ammonia, make
The zymotechnique that poultry dung feed utilizes more cleans, while may advantageously facilitate the growth of probiotics；Streptococcus thermophilus, urogaster
The addition of the anaerobic bacteria such as coccus and lactobacillus acidophilus, carbons material in excrement material can be decomposed and form the organic molecule with sour fragrance
Compound, fermentation poultry dung is produced sour fragrance, while make to contain substantial amounts of probiotics in fermentation poultry dung, into after animal intestinal tract,
Production to promoting the beneficial bacteria of intestinal tract such as Bifidobacterium, suppresses disease bacteria growing, recovers gut flora balance, strengthens intestine immunity
Function and physiological function play an important roll；
（2）This application provides the zymotechnique that a kind of fodder utilizes chicken manure, sent out using aerobic fermentation and the step of anaerobic fermentation two
Ferment technique, the pathogenic bacteria such as Shigella present in chicken manure will can be effectively eliminated during aerobic fermentation, and can be by foul smell thing
Matter NH3Transform into nitrate, H2S is changed into H2SO4, other materials can be changed into CO2And H2O, elimination stink, aspergillus niger,
Bacillus subtilis and saccharomycete can secrete a variety of enzymes and help digest and then improve food conversion ratio；In anaerobic fermentation process
In, by the fermentation of anaerobe, generate sour fragrance matter, improve the palatability of product, additionally it is possible to secrete vitamin,
The nutriments such as organic acid improve the immunity of body so as to promote the growth of cultivation livestock and poultry.Aerobic fermentation and anaerobic fermentation
Complement each other, be built into the biofermentation technique system that high-efficiency feedization utilizes chicken manure jointly, there is product preferably application to push away
Wide value；
（3）Using stepwise fermentation, fermentation strain requires different, different reaction controlling conditions to temperature, pH, logical oxygen etc., improves
The growth rate of thalline, shortens fermentation time, improves production efficiency, under the technical program, most long fermentation time is
48h, the fermentation time of prior art substantially less than in industry；
（4）Technological condition for fermentation is gentle, and production cost is low, and using solid fermentation, and fermentation period is shorter, the technical program production
Cost is only to have reported the 1/2 of technical merit at present, and lifting industrial economy benefit and large-scale promotion, which are applied, has important work
With；
（5）Using solid state fermentation, simple production process, energy consumption is low, non-environmental-pollution, investment is small, is easy to large-scale production.
Embodiment
The seed liquor of fermented bacterium in following examples uses following technique：
（1）Aspergillus niger or the seed liquid and preparation method thereof of sporotrichum thermophile bacterium：Aseptically, using potato culture medium 35~
24h is cultivated under the conditions of 40 DEG C, aspergillus niger or sporotrichum thermophile bacterium seed liquor is made, it is desirable to the living bacteria count of seed liquor >=
109cfu/mL；
（2）Saccharomycete seed liquor preparation method：Under aseptic technique, oese picking two is used from saccharomycete YDP inclined-planes
Ring strain, it is inoculated into the triangular flask of 500mL dress 300mLPDA fluid nutrient mediums, 28 DEG C~32 DEG C, 140r/min shaken cultivations
After 24h, then expand culture according to above-mentioned technique inoculation seeding tank and prepare saccharomycete seed liquor, it is desirable to effective viable bacteria of seed liquor
Number >=109cfu/mL；
（3）Bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquid and preparation method thereof：Aseptically, cultivated using LB
Base cultivates 24h under the conditions of 28~32 DEG C, and liquid bacillus seed liquor is made, it is desirable to and the living bacteria count of seed liquor >=
109cfu/mL；
（4）Streptococcus thermophilus, urine enterococcus and the seed of lactobacillus acidophilus liquid and preparation method thereof：By inclined-plane or glycerol tube preservation of bacteria strain
By corresponding seed culture medium, wherein clostridium butyricum is seeded to LB culture mediums, and lactic acid bacteria culturers are seeded to MRS trainings
Support in base, 37 DEG C of 20~30h of quiescent culture obtain primary seed solution；Then primary seed solution is inoculated into 10L vial kind
In sub- fermentation tank, 37 DEG C of 20~30h of fermentation obtain secondary seed solution, it is desirable to living bacteria count >=10 of seed liquor9cfu/mL。
Embodiment 1
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are candida utili
With molasses yeast 1:0.75 Mixed Microbes；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus 1:1:3 mixing
Flora；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 2
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, and the saccharomycete is candida utili and molasses yeast 1:0.75 Mixed Microbes；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus 1:1:3 mixing
Flora；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 3
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is sporotrichum thermophile bacterium, and the saccharomycete is candida utili and molasses yeast 1:0.75 it is mixed
Close bacterium；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus 1:1:3 mixing
Flora；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 4
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are Candida utilis ferment
It is female；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus 1:1:3 mixing
Flora；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 5
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are molasses yeast；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus 1:1:3 mixing
Flora；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 6
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are candida utili
With molasses yeast 1:0.75 Mixed Microbes；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is streptococcus thermophilus；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 7
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are candida utili
With molasses yeast 1:0.75 Mixed Microbes；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is urine enterococcus；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment 8
A kind of fodder utilizes the bio-fermentation process of chicken manure, and concrete technology step includes：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is the 4wt% of solid medium, mixes rear compost fermentation thoroughly, is led to
Wind culture 27h；The mould is aspergillus niger, sporotrichum thermophile bacterium 1:1 mixed bacteria liquid, the saccharomycete are candida utili
With molasses yeast 1:0.75 Mixed Microbes；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.8wt%, anaerobic fermentation 14h；Wherein described anaerobic bacteria is lactobacillus acidophilus；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
Embodiment illustrates the technique effect of the application acquirement with test data below 9
1st, index determining method
Nutritional ingredient index determining：Take sample 100g to be placed in 105 DEG C of drying boxes and dry 10h or so, untill constant weight, grinding
Powdering, wherein total protein use national standard（GB/T6432-1994）Kjeldahl nitrogen determination, true albumen are sunk with copper sulfate method
According to national standard after the albumen of shallow lake（GB/T6432-1994）Kjeldahl nitrogen determination, coarse-fibred content acid-alkali washing-weight
Method is quickly determined, and the detection of total amino acid is measured using formaldehyde method；
Lactic acid content determines：Lactic acid content measure uses acid-base titration in fermentation poultry dung.The extracting method of lactic acid in sample：It is accurate
The tunning 2g after drying is really weighed, is placed in 100mL beakers, adds 50mL ultra-pure waters, after standing 24h under the conditions of 4 DEG C,
Filter in 150mL conical flasks, instill 2~3 drop phenolphthalein indicators, it is micro- to be titrated to solution with 0.1mol/L sodium hydroxide solution
It is red.Lactic acid content calculation formula：
Lactic acid content（%）=
In formula：C is naoh concentration, and V is that sample liquid consumes sodium hydroxide volume, V0Sodium hydroxide volume is consumed for blank, M is
Lactic acid molal weight, m are that sample weighs quality.
2nd, experiment packet
Experiment is divided into embodiment 1-8 test groups, blank control group（Redistilled water replaces fermentation seed liquid, the same embodiment of remaining technique
1）, control 1 group（Aerobic flora only contains mould, and mould, anaerobic bacteria flora composition and ratio, fermentation manufacturing technique is the same as embodiment 1）、
Compare 2 groups（Aerobic flora only contains saccharomycete, and saccharomycete, anaerobic bacteria flora composition and ratio, fermentation manufacturing technique is the same as embodiment 1）、
Compare 3 groups（Aerobic flora is only bacillus subtilis, and anaerobic bacteria flora composition and ratio, fermentation manufacturing technique are the same as embodiment 1）, it is right
According to 4 groups（Aerobic flora only contains thermophilic heterotrophic ammonia-oxidizing bacterium, saccharomycete, anaerobic bacteria flora composition and ratio, fermentation manufacturing technique
With embodiment 1）；Chicken manure raw material is that index nutrient component difference is not notable after testing with a collection of raw material in above-mentioned packet；
3rd, data analysis statistical
The random single factor analysis of data application SPSS11.5 softwares, and LSD multiple comparison graphs are used, with " average value ± standard
Difference（SD）" represent.
4th, result of the test
Influence of the different technology conditions of table 2 to fermentation poultry dung nutritive index
| Testing index | Crude protein（%） | True albumen（%） | Crude fibre（%） | Amino acid（%） | Lactic acid（%） |
| Blank control group | 29.17±0.71 | 13.77±0.46 | 15.02±0.44 | 8.11±0.38 | 0.43±0.08 |
| The test group of embodiment 1 | 32.09±0.65 | 22.81±0.53 | 6.13±0.29 | 18.27±0.25 | 7.54±0.28 |
| The test group of embodiment 2 | 29.74±0.51 | 19.62±0.42 | 11.85±0.35 | 10.27±0.32 | 3.96±0.31 |
| The test group of embodiment 3 | 28.78±0.62 | 18.14±0.56 | 10.87±0.29 | 9.93±0.28 | 4.08±0.25 |
| The test group of embodiment 4 | 30.77±0.48 | 18.46±0.55 | 7.91±0.41 | 13.17±0.44 | 5.21±0.37 |
| The test group of embodiment 5 | 29.48±0.65 | 16.29±0.49 | 8.43±0.52 | 12.09±0.52 | 4.84±0.26 |
| The test group of embodiment 6 | 31.74±0.72 | 20.70±0.51 | 7.06±0.38 | 15.08±0.46 | 2.11±0.18 |
| The test group of embodiment 7 | 30.52±0.69 | 19.88±0.63 | 7.22±0.42 | 14.79±0.39 | 1.47±0.15 |
| The test group of embodiment 8 | 31.19±0.44 | 20.21±0.59 | 7.14±0.45 | 14.96±0.41 | 3.69±0.22 |
| Compare 1 group | 24.78±0.86 | 15.29±0.68 | 10.65±0.55 | 9.76±0.33 | 4.92±0.32 |
| Compare 2 groups | 29.29±0.92 | 17.48±0.66 | 14.29±0.49 | 10.21±0.37 | 3.14±0.29 |
| Compare 3 groups | 26.91±0.78 | 15.41±0.71 | 12.95±0.61 | 11.35±0.41 | 4.74±0.41 |
| Compare 4 groups | 24.08±0.88 | 14.37±0.69 | 14.88±0.39 | 9.63±0.32 | 2.07±0.36 |
Above-mentioned result of the test shows that the selection of aerobic fermentation flora and anaerobic fermentation flora can produce significant shadow to fermentation poultry dung
Ring.Comparing embodiment nutritional ingredient index, embodiment 1 are the application most preferred embodiment.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to foregoing reality
Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to foregoing reality
Apply the technical scheme described in example to modify, or equivalent substitution is carried out to which part technical characteristic；And to these modifications
Or replace, the essence of appropriate technical solution is departed from the spirit and scope of claimed technical solution of the invention.
Claims (9)
1. a kind of fodder utilizes the bio-fermentation process of chicken manure, mixed bacterium aerobic fermentation and the mixed step of bacterium anaerobic fermentation two are specifically included
Fermentation, wherein aerobic fermentation flora include mould, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium；Anaerobic fermentation
Flora includes one or more of mixtures in streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus.
2. a kind of fodder according to claim 1 utilizes the bio-fermentation process of chicken manure, it is characterised in that described aerobic
Mould, saccharomycete, bacillus subtilis, the amount ratio of thermophilic heterotrophic ammonia-oxidizing bacterium are 1.2 in fermentative microflora:1.8:0.6:1.
3. a kind of fodder according to claim 1-2 utilizes the bio-fermentation process of chicken manure, it is characterised in that described mould
Bacterium is aspergillus niger, one or two kinds of Mixed Microbes of sporotrichum thermophile bacterium.
4. a kind of fodder according to claim 1-2 utilizes the bio-fermentation process of chicken manure, it is characterised in that the ferment
Female bacterium can be Mixed Microbes one or more of in candida utili, brewer's yeast, saccharomyces cerevisiae, molasses yeast.
5. a kind of fodder according to claim 1 utilizes the bio-fermentation process of chicken manure, it is characterised in that the yeast
The optimal strain of bacterium is the Mixed Microbes of candida utili and molasses yeast, and its amount ratio is 1:0.75.
6. a kind of fodder according to claim 1 utilizes the bio-fermentation process of chicken manure, it is characterised in that the anaerobism
The optimal flora of fermentative microflora is streptococcus thermophilus, urine enterococcus and lactobacillus acidophilus inoculative proportion 1:1:3 mixed bacterial.
7. a kind of fodder according to claim 1 utilizes the bio-fermentation process of chicken manure, it is characterised in that the black song
The preparation method of mould or sporotrichum thermophile bacterium seed liquor is：Aseptically, using potato culture medium in 35~40 DEG C of bars
24h is cultivated under part, aspergillus niger or sporotrichum thermophile bacterium seed liquor is made, it is desirable to living bacteria count >=10 of seed liquor9cfu/mL；
The saccharomycete seed liquor preparation method is：Under aseptic technique, the ring of oese picking two is used from saccharomycete YDP inclined-planes
Strain, it is inoculated into the triangular flask of 500mL dress 300mLPDA fluid nutrient mediums, 28 DEG C~32 DEG C, 140r/min shaken cultivations 24h
Afterwards, then expand culture according to above-mentioned technique inoculation seeding tank and prepare saccharomycete seed liquor, it is desirable to the living bacteria count of seed liquor
≥109cfu/mL；The bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquid and preparation method thereof are：Aseptically,
24h is cultivated under the conditions of 28~32 DEG C using LB culture mediums, liquid bacillus seed liquor is made, it is desirable to effective work of seed liquor
Bacterium number >=109cfu/mL；The streptococcus thermophilus, urine enterococcus and the seed of lactobacillus acidophilus liquid and preparation method thereof are：By inclined-plane
Or glycerol tube preservation of bacteria strain is seeded to LB culture mediums, lactic acid by corresponding seed culture medium, wherein clostridium butyricum
Bacterium strain is seeded in MRS culture mediums, and 37 DEG C of 20~30h of quiescent culture obtain primary seed solution；Then primary seed solution is connect
Kind is into 10L vial seed fermentation tank, and 37 DEG C of 20~30h of fermentation obtain secondary seed solution, it is desirable to effective work of seed liquor
Bacterium number >=109cfu/mL。
8. a kind of fodder according to claim 1 utilizes the bio-fermentation process of chicken manure, it is characterised in that the feed
Change is included using the bio-fermentation process concrete technology step of chicken manure：
（1）Dispensing：According to 80 parts of chicken manure, 10 parts of corn flour, 5 parts of wheat bran, 0.1 part of ammonium sulfate, 2 parts of urea, potassium dihydrogen phosphate
0.05 part, the ratio of 0.03 part of magnesium sulfate be well mixed, adjust the water content 40%~50% of material, dry and wet standard with hand-tight to be held
Water, which can just be oozed, to be advisable；
（2）Aerobic fermentation：The mould activated, saccharomycete, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are pressed
1.2:1.8:0.6:1 ratio is seeded to culture medium, and total inoculum concentration is 3~5wt% of solid medium, mixes rear compost fermentation thoroughly,
24~30h of aerlbic culture；
（3）Anaerobic fermentation：Stop ventilation after aerobic fermentation, anaerobic fermentation bacterial is inoculated with after 2h, inoculum concentration is solid medium
1.5~2wt%, 12~16h of anaerobic fermentation；
（4）Dry：Low-temperature aeration is dried after fermentation ends；
（5）Crush：Dried fermentation poultry dung is crushed and produces fermentation poultry dung feed.
9. a kind of fodder according to claim 8 utilizes the bio-fermentation process of chicken manure, it is characterised in that the biology
The chicken manure of the preferred chick of chicken manure in zymotechnique.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108531411A (en) * | 2018-04-23 | 2018-09-14 | 中国科学院成都生物研究所 | A kind of combination bacteria preparation of high-efficiency fermenting chicken manure |
| CN108634090A (en) * | 2018-04-23 | 2018-10-12 | 中国科学院成都生物研究所 | A kind of chicken manure fermenting method that true protein content is high |
| CN109020727A (en) * | 2018-10-12 | 2018-12-18 | 江西省农业科学院植物保护研究所 | A kind of bio-organic fertilizer and preparation method thereof preventing Asparagus Stem Blight |
| CN109160853A (en) * | 2018-10-28 | 2019-01-08 | 青岛嘉瑞生物技术有限公司 | A kind of biological organic fertilizer of efficient prevention and treatment rice disease |
| CN109400384A (en) * | 2018-12-19 | 2019-03-01 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN109825460A (en) * | 2019-03-29 | 2019-05-31 | 上海华杰生态环境工程有限公司 | The composite bacteria agent and preparation method thereof of Rapid Fermentation human and animal excreta |
| CN113142387A (en) * | 2021-02-08 | 2021-07-23 | 扬州翔龙禽业发展有限公司 | Chicken manure recycling treatment method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108531411A (en) * | 2018-04-23 | 2018-09-14 | 中国科学院成都生物研究所 | A kind of combination bacteria preparation of high-efficiency fermenting chicken manure |
| CN108634090A (en) * | 2018-04-23 | 2018-10-12 | 中国科学院成都生物研究所 | A kind of chicken manure fermenting method that true protein content is high |
| CN109020727A (en) * | 2018-10-12 | 2018-12-18 | 江西省农业科学院植物保护研究所 | A kind of bio-organic fertilizer and preparation method thereof preventing Asparagus Stem Blight |
| CN109020727B (en) * | 2018-10-12 | 2021-09-14 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing stem blight of asparagus and preparation method thereof |
| CN109160853A (en) * | 2018-10-28 | 2019-01-08 | 青岛嘉瑞生物技术有限公司 | A kind of biological organic fertilizer of efficient prevention and treatment rice disease |
| CN109400384A (en) * | 2018-12-19 | 2019-03-01 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN109400384B (en) * | 2018-12-19 | 2022-02-25 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN109825460A (en) * | 2019-03-29 | 2019-05-31 | 上海华杰生态环境工程有限公司 | The composite bacteria agent and preparation method thereof of Rapid Fermentation human and animal excreta |
| CN113142387A (en) * | 2021-02-08 | 2021-07-23 | 扬州翔龙禽业发展有限公司 | Chicken manure recycling treatment method and application |
| CN113142387B (en) * | 2021-02-08 | 2023-09-08 | 扬州翔龙禽业发展有限公司 | Chicken manure recycling treatment method and application |
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