CN105483050A - Lactobacillus plantarum strain and application thereof in fermented feed - Google Patents

Lactobacillus plantarum strain and application thereof in fermented feed Download PDF

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CN105483050A
CN105483050A CN201511029973.9A CN201511029973A CN105483050A CN 105483050 A CN105483050 A CN 105483050A CN 201511029973 A CN201511029973 A CN 201511029973A CN 105483050 A CN105483050 A CN 105483050A
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powder
fermented
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lactobacillusplantarum
plant lactobacillus
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江衍哲
余际国
郭庆
王宏
张虎
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Beijing Yinghuir Biotechnology Co Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
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    • C12R2001/225Lactobacillus
    • C12R2001/25Lactobacillus plantarum
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Abstract

The invention discloses a lactobacillus plantarum strain. The lactobacillus plantarum strain is characterized by being preserved in the China General Microbiological Culture Collection Center (CGMCC) with the register number of CGMCCNo.11721 and the preservation date of Nov. 23rd, 2015, and the CGMCC is located in the Institute of Microbiology, Chinese Academy of Science, No.3, first yard, Beichenxi Road, Chaoyang District, Beijing. The strain has high growth performance, high acid production performance and high bacteriostatic performance, and products fermented by means of the strain have a good feeding effect. Growth of enteric pathogenic bacteria can be inhibited through lactic acid metabolism, so that the immunity of livestock is improved remarkably, the intestinal microecological environment is improved, morbidity is reduced, and the digestion rate and the milk yield are increased to a certain extent.

Description

One lactobacillus plantarum and the application in fermented feed thereof
Technical field
The present invention relates to microbe application and feed preparation manufacture field, particularly relating to a kind of for promoting the plant lactobacillus of animal immunizing power and the application in fermented feed thereof.
Background technology
Fermented feed has good palatability, safe, pollution-free, drug residue free, the feature such as easy to digest.By feeding, fermented feed can promote animal immunizing power, reduces the generation of disease, reduces diarrhea rate, mortality ratio etc., can reduce or the use of substitute antibiotics.
Plant lactobacillus fermenting process can utilize the lactose in raw material to produce lactic acid, and lactic acid is combined the absorption that can promote calcium with calcium, and therefore for the colony having lactose intolerance, be especially in and generate budding colony fast, fermented feed has great advantage.Feed creates various lactobacillus meta-bolites through lactobacillus-fermented, and comprise vitamins, enzyme and various biologically active substances etc., fermented feed is easier to digest and assimilate.But the fermentation capacity of current plant lactobacillus is limited, the production application poor effect in fermented feed.
Summary of the invention
In view of this, the object of the invention is to proposition one lactobacillus plantarum and the application in fermented feed thereof, to promote the immunizing power of animal, reduce the generation of disease, reduce diarrhea rate, mortality ratio etc.
Based on above-mentioned purpose, plant lactobacillus Lactobacillusplantarum provided by the invention is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation place is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, what preservation was registered on the books is numbered CGMCCNo.11721, and preservation date is on November 23rd, 2015.
The present invention also provides the application of a kind of above-mentioned thing Bacterium lacticum Lactobacillusplantarum in fermented feed.
In some embodiments of the invention, described application comprises:
Add water mixing in cereal materials, and obtained fermention medium, is inoculated in described plant lactobacillus Lactobacillusplantarum in described fermention medium and ferments, obtained fermented liquid;
Non-fermented raw mixture is mixed with fermented liquid, obtained fermented feed.
In some embodiments of the invention, described plant lactobacillus Lactobacillusplantarum and Saccharomyces Cerevisiae in S accharomycescerevisiae is inoculated in described fermention medium carries out cooperative fermentation.
In some embodiments of the invention, described yeast is that Saccharomyces Cerevisiae in S accharomycescerevisiaeSa-10 has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, CGMCCNo.6120.
In some embodiments of the invention, described cereal materials is popcorn, dehulled soybean meal or fermented bean dregs, sweet potato powder or analysis for soybean powder, rice flour or Semen setariae powder, whole meal flour, and the weight percentage of described popcorn, dehulled soybean meal or fermented bean dregs, sweet potato powder or analysis for soybean powder, rice flour or Semen setariae powder, whole meal flour and water is followed successively by 1 ~ 20%, 1 ~ 50%, 1 ~ 20%, 1 ~ 20%, 1 ~ 20% and 50 ~ 90%.
In some embodiments of the invention, described cereal materials is rice flour, analysis for soybean powder and Semen Maydis powder, and the weight percentage of described rice flour, analysis for soybean powder and Semen Maydis powder is followed successively by 1 ~ 30%, 1 ~ 30% and 40 ~ 80%.
In some embodiments of the invention, the inoculum size of described plant lactobacillus Lactobacillusplantarum is 1% ~ 3%.
In some embodiments of the invention, described non-fermented raw mixture comprises amino acid, VITAMIN, trace element, salt, stone flour, monocalcium phosphate, white sugar and super steam fish meal.
Bacterial classification provided by the invention growth, produce sour, antibacterial etc. in show good performance, its leavened prod has good feeding effect.Its metabolism lactic acid can suppress enteropathogenic bacteria growing, thus significantly can promote the immunizing power of livestock and poultry, improves intestine microenvironment, reduces sickness rate, and brings certain lifting to the aspect such as digestibility, milk yield.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, the present invention is described in more detail.
The separation andpreconcentration of embodiment 1 plant lactobacillus Lactobacillusplantarum
Described plant lactobacillus EM-12 bacterial strain of stating is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 23rd, 2015, deposit number is: CGMCCNo.11721, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
Plant lactobacillus EM-12 provided by the invention makes separation screening in fermentation cow's milk by oneself from the Inner Mongol four prince's flag herdsman to obtain, and concrete isolation identification step is as follows:
1) get rice meal, analysis for soybean powder and Semen Maydis powder mixs by weight the ratio of 1:1:1, get said mixture and to add water mixing according to the ratio of material water weight ratio 1:4, obtain substratum;
2) get the fermentation milk sample containing viable bacteria, the inoculum size according to 2% is inoculated in described substratum, fully shakes mixing, 36 DEG C of anaerobism, quiescent culture, incubation time 24 hours; Above-mentioned nutrient solution is repeatedly gone down to posterity 3 times in this substratum, obtains the lactobacillus strain being applicable to fermented cereal class;
3) get above-mentioned nutrient solution 1mL, inoculation gradient dilution also coats the dull and stereotyped upper 36 DEG C of cultivation 72h of MRS; Picking individual colonies meets bacterium 36 DEG C of cultivation 18h in MRS liquid nutrient medium, uses in order to preservation and qualification.
4) bacterial classification is delivered to Institute of Microorganism, Academia Sinica to identify, comprehensively analyze according to bacterium cell form, biochemical character, 16SrRNA gene order, pheS gene order etc., be accredited as plant lactobacillus (Lactobacillusplantarum).
Cellular form and the physical and chemical experiment of this bacterial strain the results are shown in following table:
16SrDNA sequencing results:
GCGTGCTAATACATGCAGTCGAACGAACTCTGGTATTGATTGGTGCTTGCATCATGATTTACATTTGAGTGAGTGGCGAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCGGGGGATAACACCTGGAAACAGATGCTAATACCGCATAACAACTTGGACCGCATGGTCCGAGTTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTAGATGGTGGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTGACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTCAACCGAAGAAGTGCATCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTGCAGCTAACGCATTAAGCATTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAATCTAAGAGATTAGACGTTCCCTTCGGGGACATGGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATCAGTTGCCAGCATTAAGTTGGGCACTCTGGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAACGAGTTGCGAACTCGCGAGAGTAAGCTAATCTCTTAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTAGGAACCAGCCGCCTAAGTGACAGA
PheS gene sequencing result:
GGCGACAATTACAAGACGTGCTACTACGCACGCAGACGTCTGCTGATCAGCCGCGGTCACTTGAAAATCACGATTTTTCTAAAGGACCGCTGAAGGTCTTGTCACCTGGCCGCGTTTATCGGCGTGATACGGATGATGCAACCCATTCCCATCAATTTCATCAAATTGAAGGGTTAGTCGTGGACAAGCATATTACGATGGCTGATTTGAAGGGCACCTTAATTCTGGTTGCCAAGACTTTGTTTGGCGATCAATTCGATGTTCGGCTACGGCCAAGCTTCTTTCCATTCACGGAACCATCCGTAGAAGCTGATGTAACTTGCTTTAATTGCAATGGCAAGGGCTGTGCAATCTGTAAGCAAACGGGTTGGATCGAAGTACTGGGTGCCGGCATGGTTCACCCCCACGTGTTAGAAATGTCTGGCATTGATCCAGAAGAATATGGTGGTTTTGCTTTCGGTCTAGGGA
The mensuration of embodiment 2 plant lactobacillus EM-12 thalli growth performance and product acid activity
(1) preparation of substratum:
Get a certain amount of cereal materials and be crushed to 1.0mm, to add water mixing in the ratio of material water weight ratio 1:3, then by mixed liquor high-temperature sterilization, then be cooled to about 36 DEG C, acquisition fermention medium.Wherein, described sterilising conditions is 121 DEG C of sterilizings, 20 minutes time.Described cereal materials comprises rice meal, analysis for soybean powder and Semen Maydis powder, and the weight ratio of described rice flour, analysis for soybean powder and Semen Maydis powder is 1:1:1.
(2) acquisition of fermented bacterium:
Get this lactobacillus strain of glycerine pipe preservation, be seeded to MRS substratum, 36 DEG C of quiescent culture 20 hours, obtain lactobacillus strain.Wherein, described MRS liquid nutrient medium is composed as follows: 10g peptone, 5g extractum carnis, 4g yeast leaching powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL tween 80,0.2g magnesium sulfate, 0.05g manganous sulfate add 1000mL distilled water, regulate pH to 6.5,121 DEG C of sterilizing 20min.
(3) by obtained lactobacillus strain by volume 2% inoculum size be inoculated in the fermention medium of step (1), 36 DEG C of standing Anaerobic culturel, incubation time 20 hours, obtain fermented liquid.Get after fermention medium dilutes 10 times, under 600nm, measure absorbance, absorbance is 0.76 ± 0.02.Get fermented liquid centrifugal mensuration pH and lactic acid content, wherein pH value is 3.80 ± 0.05, lactic acid content 7.5 ± 0.5g/L.
The mensuration of comparative example 2 plant lactobacillus type strain product acid activity
Get China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center plant lactobacillus type strain, measure its product acid activity according to the step (1) of embodiment 2, (2) and (3).
(1) with the step (1) of embodiment 2.
(2) with the step (2) of embodiment 2.
(3) get after seed culture medium dilutes 10 times, under 600nm, measure absorbance, absorbance is 0.62 ± 0.04; Get fermented liquid centrifugal mensuration pH and lactic acid content, wherein pH value is 4.10 ± 0.05, lactic acid content 6.0 ± 0.5g/L.
Visible, plant lactobacillus EM-12 provided by the invention has higher product acid activity, can make an addition in feed as souring agent.
The guaranteeing the quality of embodiment 3 plant lactobacillus EM-12, antipollution experiment
(1) non-fermented raw mixture is prepared: by weight percentage, amino acid 2%, VITAMIN 0.5%, trace element 0.5%, salt 1.0%, stone flour 3.0%, monocalcium phosphate 3.0%, white sugar 30% and super steam fish meal 60% are mixed, and to be crushed to granularity be 1.0mm, obtained non-fermented raw mixture.
(2) fermented liquid is prepared according to the step of embodiment 2.
(3) be 70%, 30% to mix fermented liquid and non-fermented raw mixture according to weight percentage, obtain fermented soln.
(4) adopt plastics Soft Roll to pack, adopt the general pasteurization of food service industry to carry out sterilizing, sterilization time controlled at 60 minutes.
(5) guarantee the quality, antipollution experiment
1. the fermented soln of acquisition is poured in triangular flask, the uncovered incubator being placed in 30 DEG C of juxtaposition; 2. solution is poured in aluminium foil bag, and heat sealing, be placed in 30 DEG C of incubators.Wherein, the feed liquid 1. keeps 72 hours, and free from extraneous odour, pH are unchanged.2. without swollen bag, free from extraneous odour, pH were unchanged in 72 hours for the aluminium foil bag in.
What comparative example 3 plant lactobacillus type strain product was sour guarantees the quality, antipollution experiment
Get China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center plant lactobacillus type strain, carry out guaranteeing the quality according to the step (1) of embodiment 3, (2), (3), (4) and (5), antipollution experiment.
(1)-(4) are with step (1)-(4) of embodiment 3.
(5) guarantee the quality, antipollution experiment
1. the fermented soln of acquisition is poured in triangular flask, the uncovered incubator being placed in 30 DEG C of juxtaposition; 2. solution is poured in aluminium foil bag, and heat sealing, be placed in 30 DEG C of incubators.Wherein, the feed liquid 1. keeps 72 hours, and smell acid is smelly, and pH significantly declines.2. the 72 hours obvious swollen bags of the aluminium foil bag in, smell acid is smelly, pH significantly declines.
Visible, plant lactobacillus EM-12 provided by the invention have guarantee the quality preferably, contamination resistance.
It should be noted that, in another embodiment, the preparation of described non-fermented raw mixture comprises the following steps: by weight percentage, amino acid 3%, VITAMIN 0.4%, trace element 0.6%, salt 1.5%, stone flour 2.3%, monocalcium phosphate 3.6%, white sugar 38.6% and super steam fish meal 50% are mixed, and to be crushed to granularity be 1.2mm, obtained non-fermented raw mixture.
The feeding effect of embodiment 4 fermented feed
The fermented feed prepared by aforesaid method is used for the feeding experiment of weanling pig, and the uniform 28 age in days weanling pigs of Stochastic choice 100 health, body weight, are divided into control group and test group, often organizes 5 repetitions, each repetition 10,14 days trial periods.Control group fed powder, test group within first 4 days, feed fermentation liquid sucking pig material, within 5-7 days, be transited into the powder identical with control group gradually, the powder identical with control group of feeding for 8-14 days.The body weight of statistics piglet and diarrhoea data are as following table.
As seen from the above table, the piglet of liquid material of feeding comparatively is fed the many weightening finishes 9.0% (1.29kg) of piglet of powder, and diarrhea rate reduces by 70.6%.
The present invention significantly can promote the immunizing power of livestock and poultry, improves intestine microenvironment, reduces diarrhea rate.
Embodiment 5 works in coordination with the fermentation situation of yeast
Spreading cultivation of fermented bacterium: select yeast and plant lactobacillus EM-12, cultivate spread cultivation through primary inclined plane cultivation, two-stage liquid respectively, step is as follows:
(1) primary inclined plane is cultivated
Bacterium lacticum is transferred in MRS medium slant, cultivate 24 hours in 36 DEG C; Yeast saccharomyces cerevisiae is transferred in YPD medium slant, cultivate 20 hours in 30 DEG C;
(2) secondary liquid is cultivated
Get the fresh Bacterium lacticum inclined plane inoculating that step (1) obtains to cultivate in liquid nutrient medium, seal with aseptic sealed membrane, be placed in constant incubator, 36 DEG C of quiescent culture 20h obtain one-level Bacterium lacticum seed liquor, get primary seed solution in 5% ratio be seeded in secondary seed medium, the same first order seed of culture condition, obtains the seed liquor of Bacterium lacticum;
Getting the fresh yeast saccharomyces cerevisiae inclined-plane that step (1) obtains is forwarded in liquid nutrient medium, 30 DEG C of shaking tables cultivate 18 hours, obtain one-level yeast saccharomyces cerevisiae seed liquor, get primary seed solution and be inoculated in secondary seed medium by 5%, the same first order seed of culture condition; Obtain the seed liquor of yeast saccharomyces cerevisiae.
(3) preparation of substratum:
Get a certain amount of cereal materials and be crushed to 1.0mm, to add water mixing in the ratio of material water weight ratio 1:4, by mixed liquor high-temperature sterilization, and be cooled to about 36 DEG C, acquisition fermention medium.Wherein, described sterilising conditions is 121 DEG C of sterilizings, 20 minutes time.Described cereal materials comprises rice meal, analysis for soybean powder and Semen Maydis powder, and the weight ratio of described rice flour, analysis for soybean powder and whole meal flour is 3:1:1.
(4) get the seed liquor of yeast saccharomyces cerevisiae, the seed liquor of lactobacillus strain respectively, access same fermention medium by the inoculum size of total inoculum size 4%.Culture temperature 34 DEG C, shaking speed 200rpm.Wherein, yeast and Bacterium lacticum inoculate volume ratio 1:1.Ferment 20 hours, yeast viable count is 1.1 × 10 8cfu/ml, living preparation of lactobacillus number 2.2 × 10 9cfu/ml.
In the present embodiment, described yeast is yeast saccharomyces cerevisiae (Saccharomycescerevisiae) Sa-10, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, CGMCCNo.6120.
Comparative example 5
Plant lactobacillus EM-12 is replaced with China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center plant lactobacillus type strain, other steps are with embodiment 5.Ferment 20 hours, yeast viable count is 0.8 × 10 8cfu/ml, living preparation of lactobacillus number 1.8 × 10 9cfu/ml.
Visible, this bacterial strain has superior growth performance more when the probiotic bacterium of collaborative yeast class carries out fermentation culture, is therefore more suitable for the cooperative fermentation with yeast class bacterial classification, having and more having research and development, using potentiality when preparing multiple bacteria compound fermentation feed.
Bacterial classification provided by the invention growth, produce sour, antibacterial etc. in show good performance, its leavened prod has good feeding effect.Its metabolism lactic acid can suppress enteropathogenic bacteria growing, thus significantly can promote the immunizing power of livestock and poultry, improves intestine microenvironment, reduces sickness rate, and brings certain lifting to the aspect such as digestibility, milk yield.
Those of ordinary skill in the field are to be understood that: the discussion of above any embodiment is only exemplary, and not intended to be implies that the scope of the present disclosure (comprising claim) is limited to these examples; Under thinking of the present invention, also can combine between technical characteristic in above embodiment or different embodiment, step can realize with random order, and there are other changes many of different aspect of the present invention as above, and they do not provide in details for the sake of simplicity.Therefore, within the spirit and principles in the present invention all, any omission made, amendment, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (9)

1. a lactobacillus plantarum Lactobacillusplantarum, it is characterized in that, described bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation place is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, what preservation was registered on the books is numbered CGMCCNo.11721, and preservation date is on November 23rd, 2015.
2. the application of a plant lactobacillus Lactobacillusplantarum as claimed in claim 1 in fermented feed.
3. application according to claim 2, is characterized in that, comprising:
Add water mixing in cereal materials, and obtained fermention medium, is inoculated in described plant lactobacillus Lactobacillusplantarum in described fermention medium and ferments, obtained fermented liquid;
Non-fermented raw mixture is mixed with fermented liquid, obtained fermented feed.
4. application according to claim 3, is characterized in that, is all inoculated in described fermention medium by described plant lactobacillus Lactobacillusplantarum and Saccharomyces Cerevisiae in S accharomycescerevisiae and carries out cooperative fermentation.
5. application according to claim 4, is characterized in that, described yeast is that Saccharomyces Cerevisiae in S accharomycescerevisiaeSa-10 has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, CGMCCNo.6120.
6. application according to claim 3, it is characterized in that, described cereal materials is popcorn, dehulled soybean meal or fermented bean dregs, sweet potato powder or analysis for soybean powder, rice flour or Semen setariae powder, whole meal flour, and the weight percentage of described popcorn, dehulled soybean meal or fermented bean dregs, sweet potato powder or analysis for soybean powder, rice flour or Semen setariae powder, whole meal flour and water is followed successively by 1 ~ 20%, 1 ~ 50%, 1 ~ 20%, 1 ~ 20%, 1 ~ 20% and 50 ~ 90%.
7. application according to claim 3, is characterized in that, described cereal materials is rice flour, analysis for soybean powder and Semen Maydis powder, and the weight percentage of described rice flour, analysis for soybean powder and Semen Maydis powder is followed successively by 1 ~ 30%, 1 ~ 30% and 40 ~ 80%.
8. the application according to claim 3 or 4, is characterized in that, the inoculum size of described plant lactobacillus Lactobacillusplantarum is 1% ~ 3%.
9. application according to claim 3, is characterized in that, described non-fermented raw mixture comprises amino acid, VITAMIN, trace element, salt, stone flour, monocalcium phosphate, white sugar and super steam fish meal.
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CN106578450A (en) * 2016-11-25 2017-04-26 南宁学院 Micro-ecologic preparation for improving immunity of calves, and preparation method and application thereof
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CN107263646A (en) * 2017-07-28 2017-10-20 安徽宏润工艺品有限公司 A kind of drying Bracking-resistant method of rattan
CN109022313A (en) * 2018-08-02 2018-12-18 中国农业科学院兰州兽医研究所 One lactobacillus plantarum
CN110283749A (en) * 2019-07-02 2019-09-27 北京科拓恒通生物技术股份有限公司 One plant of lactobacillus plantarum P-01 separated and its application
CN111004752A (en) * 2019-12-30 2020-04-14 江南大学 Ethanol-resistant lactobacillus plantarum and application thereof in fermented food
CN111593010A (en) * 2020-06-30 2020-08-28 黄河三角洲京博化工研究院有限公司 Lactobacillus plantarum and method for producing fermented feed by using same
CN111685235A (en) * 2020-06-24 2020-09-22 河南省科学院生物研究所有限责任公司 Preparation method of lactobacillus plantarum fermented liquid complete feed for piglets
CN115074261A (en) * 2022-06-28 2022-09-20 四川轻化工大学 Saccharomyces boulardii, fermentation medium thereof, ruminant feed and preparation method thereof
CN116083273A (en) * 2022-09-05 2023-05-09 新希望六和股份有限公司 Lactobacillus plantarum NHE-LpE15 and application thereof

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Denomination of invention: A Plant Lactobacillus Strain and Its Application in Fermented Feed

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