CN105211550A - A kind of preparation method of mixed culture solid state fermentation sea cucumber bait - Google Patents
A kind of preparation method of mixed culture solid state fermentation sea cucumber bait Download PDFInfo
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- CN105211550A CN105211550A CN201510673567.XA CN201510673567A CN105211550A CN 105211550 A CN105211550 A CN 105211550A CN 201510673567 A CN201510673567 A CN 201510673567A CN 105211550 A CN105211550 A CN 105211550A
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- bait
- fermentation
- sea cucumber
- powder
- lactic acid
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Abstract
The invention discloses a kind of method that mixed culture solid state fermentation prepares sea cucumber fermentation bait, with sea cucumber bait, its main component is sargassum powder, extra large blue or green powder, dregs of beans, fish meal, shrimp med, scallop edge powder, come unstuck Kelp Powder, corn flour etc. are fermentation raw material, with saccharomyces cerevisiae, bacillus, lactic acid bacteria for fermentation strain, by 3 ~ 5% bait reclosing kinds, 25 ~ 30 DEG C of conditions, solid state fermentation is carried out to raw material, obtain microbial fermentation bait for sea cucumbers.Production technology of the present invention is simply easy to operation, the extensive cost of raw material sources is low is easy to popularization, with short production cycle, good product quality, is easy to promotion and application.Apply in holothruian cultures, sea cucumber survival rate and growth performance can be improved; Can improve the ecological environment of culturing pool water body simultaneously, purify water, be a kind of desirable high-quality microbial fermentation bait for sea cucumbers.
Description
Technical field
The present invention relates to microbial fermentation technology, particularly relate to a kind of method mixing several beneficial bacterium solid state fermentation and prepare sea cucumber fermentation bait.
Background technology
In recent years, apostichopus japonicus culture industry, in northern China coastal various places fast development, has huge market potential, and becomes one of important industry of China's sea-farming gradually, achieves significant economic benefit and social benefit.In traditional breeding process, in order to reduce the carrying amount of microorganism in feed or improve the effect of disease treatment, raiser often adds antibiotic in feed, a large amount of antibiotic use not only makes the immunity degradation of stichopus japonicus itself, induction pathogenic bacteria produce drug resistance, but also bring the serious problems such as medicament residue, jeopardize food security and human health.
Summary of the invention
In order to solve the problem, the invention provides a kind of mixed bacterium and comprise the method that saccharomyces cerevisiae, bacillus and lactic acid bacteria solid state fermentation prepare sea cucumber fermentation bait, this method feed is after beneficial microbe (lactic acid bacteria, saccharomycete and bacillus etc.) fermentation process, ANFs in raw material is decomposed or transforms, and generation is easily searched for food by cultivated animals, digests, absorbed and the Small molecular nutriment of nonhazardous effect.And, feed containing more active probiotic thalline, various enzyme, metabolite at different levels, multivitamin, protein breakdown products, active small peptide, amino acid, antibacterial substance, the Immune-enhancing effect factor, somatomedin etc., plays growth promoting effects, maintains the effect of microbial population of animal intestinal tract balance after probiotics fermention.Therefore, fermented feed is a kind of environmental health type feed.Solid state fermentation be microorganism not having or substantially there is no the fermentation mode on the solid state substrate of free water, have water activity low, the insoluble height of substrate water, microorganism easily grows, and enzyme activity is high, enzyme system enrich; Sweat is extensive, does not need stringent asepsis requirements; Simple in equipment, small investment, energy consumption are low, easy to operate; Post processing is easy, pollution is few, the advantages such as basic non-wastewater discharge, plays important impetus to the health of sea cucumber and green cultivation.
The present invention is achieved through the following technical solutions:
Mixed culture solid state fermentation prepares a method for sea cucumber fermentation bait, comprises the following steps:
Step a. prepares zymophyte: cultivate saccharomyces cerevisiae, bacillus and lactic acid bacteria by YPD culture medium, broth bouillon and MRS culture medium liquid state respectively; Cultivation terminates rear saccharomyces cerevisiae, bacillus, lactic acid bacterial liquid bacterium number all reach 10
8-10
9cfu/mL; Described bacillus derives from sea cucumber enteron aisle;
Step b. prepares sea cucumber bait: be made up of following raw material according to weight percent meter, sargassum powder 20%, extra large blue or green powder 20%, fermented bean dregs 4%, fish meal 3%, shrimp med 4%, scallop edge powder 2%, the Kelp Powder 32% that comes unstuck, corn flour 6%, broken wall beer yeast powder 5%, premix 4%, adopt above-mentioned formulated sea cucumber bait, mix;
Step c is inoculated: zymophyte total amount prepared by step a is added in bait prepared by step b according to the ratio that bait weighs 3 ~ 5%;
Steps d. add water: the water content of the bait that step c is obtained reaches 40 ~ 45%, mixes, reach and hold agglomerating, land loose degree;
Step e. packs sealing: sea cucumber bait steps d obtained is put into packaging bag and sealed;
Step f. ferments: 25 ~ 30 DEG C of fermentation 6-8 days, every day period overturns once sealing packaging bag.
Wherein, the bacillus adopted in step a can produce high activity amylase.
Further, in technique scheme, lactic acid bacteria in step a, is adopted to be cheese lactic acid bacteria.
Further, in technique scheme, saccharomyces cerevisiae preferred strain number is CGMCC2.4.
Further, in technique scheme, bacillus preferred strain number is MCCC1A00791.
Further, in technique scheme, Lactobacillus casei preferred strain number is CICC6244.
Further, in technique scheme, the preferred LB broth bouillon of broth bouillon.
Further, in technique scheme, described premix is preferably by being made up of following raw material by weight: vitamin A, 0.25 part; Vitamine D3,0.05 part; Vitamin E, 1.13 parts; Vitamin K3,0.20 part; Vitamin B1,0.13 part; Vitamin B2,0.22 part; Vitamin B6,0.16 part; Vitamin B12,0.04 part; Nicotinic acid, 0.68 part; Pantothenic acid, 0.39 part; Folic acid, 0.05 part; Biotin, 0.08 part; Vitamin C, 3.13 parts; Choline Chloride, 75.00 parts; Inositol, 1.50 parts; Anhydrous cupric sulfate, 0.60 part; Ferrous sulfate monohydrate, 4.75 parts; Manganese sulfate monohydrate, 2.38 parts; Monohydrate zinc sulphate, 4.25 parts; Calcium iodate, 1.75 parts; Cobalt chloride, 0.78 part; Sodium selenite, 0.19 part; Lysine, 25.00 parts; Antioxidant, 2.50 parts; Zeolite powder, 159.83 parts; Calcium monohydrogen phosphate, 125.00 parts; Powdered rice hulls, 90.00 parts.
Further, in technique scheme, saccharomyces cerevisiae in zymophyte in step c: bacillus: the mass ratio of lactic acid bacterial liquid is 1:2:2.
Further, in technique scheme, in step e, sealing bags is the packaging bag with single exhaust valve.
Invention beneficial effect
1, the present invention adopts microbial fermentation technology, and inoculation saccharomyces cerevisiae, bacillus and lactic acid bacteria prepare sea cucumber fermentation bait by the form of solid state fermentation, improve the digestive utilization ratio of sea cucumber bait and the growth performance of sea cucumber;
2, bacillus can secrete various ectoenzyme with macromolecular substances such as decomposing protein, fat, carbohydrate, make in fermented product containing multiple compositions be absorbed by the body such as amino acid, organic acid, oligosaccharides, and the amylase of this strain secretes is significantly higher than the amylase activity of other secreted from bacillus, thus it has better ferment effect;
3, production technology of the present invention is simply easy to operation, and raw material sources is extensive, low being easy to of cost is promoted, with short production cycle, and good product quality, is easy to application and promotes;
4, after this product is applied in holothruian cultures, can improve sea cucumber survival rate and growth performance, can improve the ecological environment of culturing pool water body, purify water, be a kind of desirable, high-quality microbial fermentation bait for sea cucumbers.
Detailed description of the invention
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Wherein, Wine brewing yeast strain number is CGMCC2.4; Bacterial strain of bacillus number is MCCC1A00791; Lactobacillus casei bacterial strain number is CICC6244.Premix is made up of following raw material by weight: vitamin A, 0.25 part; Vitamine D3,0.05 part; Vitamin E, 1.13 parts; Vitamin K3,0.20 part; Vitamin B1,0.13 part; Vitamin B2,0.22 part; Vitamin B6,0.16 part; Vitamin B12,0.04 part; Nicotinic acid, 0.68 part; Pantothenic acid, 0.39 part; Folic acid, 0.05 part; Biotin, 0.08 part; Vitamin C, 3.13 parts; Choline Chloride, 75.00 parts; Inositol, 1.50 parts; Anhydrous cupric sulfate, 0.60 part; Ferrous sulfate monohydrate, 4.75 parts; Manganese sulfate monohydrate, 2.38 parts; Monohydrate zinc sulphate, 4.25 parts; Calcium iodate, 1.75 parts; Cobalt chloride, 0.78 part; Sodium selenite, 0.19 part; Lysine, 25.00 parts; Antioxidant, 2.50 parts; Zeolite powder, 159.83 parts; Calcium monohydrogen phosphate, 125.00 parts; Powdered rice hulls, 90.00 parts.
Embodiment 1
Step a, preparation zymophyte: cultivate saccharomyces cerevisiae 24h with YPD culture medium 30 DEG C; LB broth bouillon 30 DEG C cultivates bacillus 24h; MRS culture medium 37 DEG C cultivates lactic acid bacteria 48h.It is 10 that cultivation terminates rear S. cervisiae number
8cfu/mL, bacillus bacterium number is 5 × 10
8cfu/mL, lactic acid bacteria bacterium number is 2 × 10
8cfu/mL;
Wherein YPD culture medium, broth bouillon and MRS culture medium prescription are as follows:
YPD culture medium: glucose 20g, peptone 20g, yeast extract 10g, distilled water 1000mL, natural pH.
LB broth bouillon: tryptone 10g, yeast leaching powder 5g, sodium chloride 10g, distilled water 1000mL, pH6.9 ± 0.1.
MRS lactic medium: glucose 20g, peptone 10g, beef extract 10g, yeast extract 5g, K
2hPO
42g, sodium acetate 5g, ammonium citrate 2g, MgSO
40.2g, MnSO
40.05g, Tween-80 1mL, distilled water 1000mL, pH6.2 ~ 6.4.
Step b, preparation sea cucumber bait: according to the formulated sea cucumber bait of sargassum powder 20%, extra large blue or green powder 20%, fermented bean dregs 4%, fish meal 3%, shrimp med 4%, scallop edge powder 2%, the Kelp Powder 32% that comes unstuck, corn flour 6%, broken wall beer yeast powder 5%, premix 4%, then mixed;
Step c, inoculation: the ratio that various probio weighs 3% according to bait is added, saccharomyces cerevisiae: bacillus: the ratio of lactic acid bacteria is 1:2:2.
Steps d, to add water: make the water content of bait reach 42%, mix, reach and hold agglomerating, land loose degree;
Step e, pack sealing: mixed sea cucumber bait is put into the packaging bag that individual event vent valve is housed and seals;
Step f, fermentation: 25 ~ 30 DEG C ferment 7 days, and every day period overturns once sealing packaging bag.When packaging bag tympanites, bait darkens as drabon look, air bleeding valve Exhaust Gas slightly tart flavour, namely obtains fermentation bait product.
The Analyses Methods for Sensory Evaluation Results of sea cucumber fermentation bait is in Table 1-1: wherein normal diet group is the sea cucumber bait that embodiment 1 step b prepares.
The sensory evaluation of the fermentation bait of table 1-1. embodiment 1
The testing result of sea cucumber fermentation bait is in Table 1-2: wherein normal diet group is the sea cucumber bait that embodiment 1 step b prepares.
The testing result of the fermentation bait of table 1-2. embodiment 1
Embodiment 2
Step a, preparation zymophyte: cultivate saccharomyces cerevisiae 24h with YPD culture medium 30 DEG C; LB broth bouillon 30 DEG C cultivates bacillus 24h; MRS culture medium 37 DEG C cultivates lactic acid bacteria 48h.It is 10 that cultivation terminates rear S. cervisiae number
8cfu/mL, bacillus bacterium number is 8 × 10
8cfu/mL, lactic acid bacteria bacterium number is 5 × 10
8cfu/mL; Wherein YPD culture medium, broth bouillon are identical with embodiment 1 with MRS culture medium prescription;
Step b, preparation sea cucumber bait: according to the formulated sea cucumber bait of sargassum powder 20%, extra large blue or green powder 20%, fermented bean dregs 4%, fish meal 3%, shrimp med 4%, scallop edge powder 2%, the Kelp Powder 32% that comes unstuck, corn flour 6%, broken wall beer yeast powder 5%, premix 4%, mix;
Step c, inoculation: the ratio that various probio weighs 4% according to bait is added, saccharomyces cerevisiae: bacillus: the ratio of lactic acid bacteria is 1:2:2;
Steps d, to add water: make the water content of bait reach 42%, mix, reach and hold agglomerating, land loose degree;
Step e, pack sealing: mixed sea cucumber bait is put into the packaging bag that individual event vent valve is housed and seals;
Step f, fermentation: 25 ~ 30 DEG C ferment 8 days, and every day period overturns once sealing packaging bag.When packaging bag tympanites, bait darkens as drabon look, air bleeding valve Exhaust Gas slightly tart flavour, namely obtains fermentation bait product.
The Analyses Methods for Sensory Evaluation Results of sea cucumber fermentation bait is in Table 2-1: wherein normal diet group is the sea cucumber bait that embodiment 2 step b prepares.
The sensory evaluation of the fermentation bait of table 2-1. embodiment 2
The Testing index of sea cucumber fermentation bait the results are shown in Table 2-2: wherein normal diet group is the sea cucumber bait that embodiment 2 step b prepares.
The testing result of the fermentation bait of table 2-2. embodiment 2
Embodiment 3
Step a, preparation zymophyte: cultivate saccharomyces cerevisiae 24h with YPD culture medium 30 DEG C; LB broth bouillon 30 DEG C cultivates bacillus 24h; MRS culture medium 37 DEG C cultivates lactic acid bacteria 48h.It is 2 × 10 that cultivation terminates rear S. cervisiae number
8cfu/mL, bacillus bacterium number is 8 × 10
8cfu/mL, lactic acid bacteria bacterium number is 3 × 10
8cfu/mL; Wherein YPD culture medium, broth bouillon are identical with embodiment 1 with MRS culture medium prescription;
Step b, preparation sea cucumber bait: according to the formulated sea cucumber bait of sargassum powder 20%, extra large blue or green powder 20%, fermented bean dregs 4%, fish meal 3%, shrimp med 4%, scallop edge powder 2%, the Kelp Powder 32% that comes unstuck, corn flour 6%, broken wall beer yeast powder 5%, premix 4%, mix;
Step c, inoculation: the ratio that various probio weighs 5% according to bait is added, saccharomyces cerevisiae: bacillus: the ratio of lactic acid bacteria is 1:2:2;
Steps d, to add water: make the water content of bait reach 45%, mix, reach and hold agglomerating, land loose degree;
Step e, pack sealing: mixed sea cucumber bait is put into the packaging bag that individual event vent valve is housed and seals;
Step f, fermentation: 25 ~ 30 DEG C ferment 7 days, and every day period overturns once sealing packaging bag.When packaging bag tympanites, bait darkens as drabon look, air bleeding valve Exhaust Gas slightly tart flavour, namely obtains fermentation bait product.
The Analyses Methods for Sensory Evaluation Results of sea cucumber fermentation bait is in Table 3-1: wherein normal diet group is the sea cucumber bait that embodiment 3 step b prepares.
The sensory evaluation of the fermentation bait of table 3-1. embodiment 3
The Testing index of sea cucumber fermentation bait the results are shown in Table 3-2: wherein normal diet group is the sea cucumber bait that embodiment 3 step b prepares.
The testing result of the fermentation bait of table 3-2. embodiment 3
Claims (4)
1. mixed culture solid state fermentation prepares a method for sea cucumber fermentation bait, it is characterized in that comprising the following steps:
Step a. prepares zymophyte: cultivate saccharomyces cerevisiae, bacillus and lactic acid bacteria by YPD culture medium, broth bouillon and MRS culture medium liquid state respectively; Cultivation terminates rear saccharomyces cerevisiae, bacillus, lactic acid bacterial liquid bacterium number all reach 10
8-10
9cfu/mL;
Step b. prepares sea cucumber bait: according to weight percent meter, the formulated sea cucumber bait of sargassum powder 20%, extra large blue or green powder 20%, fermented bean dregs 4%, fish meal 3%, shrimp med 4%, scallop edge powder 2%, the Kelp Powder 32% that comes unstuck, corn flour 6%, broken wall beer yeast powder 5%, premix 4%, mixes;
Step c is inoculated: zymophyte total amount prepared by step a is added in bait prepared by step b according to the ratio that bait weighs 3 ~ 5%;
Steps d. add water: the water content of the bait that step c is obtained reaches 40 ~ 45%, mixes;
Step e. packs sealing: sea cucumber bait steps d obtained is put into packaging bag and sealed;
Step f. ferments: 25 ~ 30 DEG C of fermentation 6-8 days, every day period overturns once sealing packaging bag.
2. preparation method according to claim 1, is characterized in that: adopt lactic acid bacteria to be cheese lactic acid bacteria in step a.
3. preparation method according to claim 1, is characterized in that: saccharomyces cerevisiae in zymophyte in step c: bacillus: the mass ratio of lactic acid bacterial liquid is 1:2:2.
4. preparation method according to claim 1, is characterized in that: in step e, sealing bags is the packaging bag with single exhaust valve.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106889374A (en) * | 2017-02-20 | 2017-06-27 | 辽宁天牧生物科技有限公司 | A kind of sea cucumber granules feed rich in various probiotics |
| CN107183406A (en) * | 2017-05-16 | 2017-09-22 | 山东省海洋生物研究院 | A kind of microorganism live bacteria bait and its production method for shellfish hatchery |
| CN107927457A (en) * | 2017-12-13 | 2018-04-20 | 大连小萝莉服饰有限公司 | A kind of sea cucumber solid fermentation bait |
| CN108041348A (en) * | 2017-12-13 | 2018-05-18 | 大连小萝莉服饰有限公司 | A kind of preparation method of sea cucumber solid fermentation bait |
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106889374A (en) * | 2017-02-20 | 2017-06-27 | 辽宁天牧生物科技有限公司 | A kind of sea cucumber granules feed rich in various probiotics |
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| CN107927457A (en) * | 2017-12-13 | 2018-04-20 | 大连小萝莉服饰有限公司 | A kind of sea cucumber solid fermentation bait |
| CN108041348A (en) * | 2017-12-13 | 2018-05-18 | 大连小萝莉服饰有限公司 | A kind of preparation method of sea cucumber solid fermentation bait |
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
| CN113974024B (en) * | 2021-11-01 | 2024-03-19 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
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Application publication date: 20160106 |