CN106036198A - Preparation method of sea cucumber biologically fermented baits - Google Patents
Preparation method of sea cucumber biologically fermented baits Download PDFInfo
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- CN106036198A CN106036198A CN201610356459.4A CN201610356459A CN106036198A CN 106036198 A CN106036198 A CN 106036198A CN 201610356459 A CN201610356459 A CN 201610356459A CN 106036198 A CN106036198 A CN 106036198A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a preparation method of sea cucumber biologically fermented baits. Marina pseudoalteromonas and mixed effective microorganisms (EM) are inoculated into the baits to be subjected to anaerobic fermentation at 25-35 DEG C for 3-7 days. The mixed EM consists of bacillus, photosynthetic bacteria, yeasts and lactobacillus. The method comprises the step of conducting solid state fermentation on the stichopus japonicus baits via the microorganisms to prepare the fermented baits. Compared with the unfermented baits, the prepared baits are significantly increased in the attractiveness to the stichopus japonicus. Besides, the fermented baits are more comprehensive in nutrition and more conductive to the growth of the stichopus japonicus, improve the survival rates of the stichopus japonicus, improve the intestinal flora of the stichopus japonicus, and improve the digestion and absorption rates of the baits by the stichopus japonicus. At the same time, due to the addition of seaweed gel degrading bacteria, the fermented baits can effectively degrade kelp powder rich in the seaweed gel and difficult to digest by the stichopus japonicus in the baits, so that the proportion of the kelp powder in the baits can be increased to replace parts of expensive sargassum and the purposes of lowering enterprise costs is acheived.
Description
Technical field
The present invention relates to a kind of fermentation bait, particularly relate to the mixing EM bacterium fermentation sea containing ocean Pseudoalteromonas
The preparation method of ginseng bait.
Background technology
Radix Morinae Bulleyanae body wall is rich in abundant collagen protein and polysaccharide, and containing various trace elements such as calcium, ferrum, manganese, has
There is higher edible and medical value, be one of important marine products economic animal of northern China.Along with apostichopus japonicus culture industry is intensive,
Scale, the fast development of automatization, limited natural bait can not meet the needs of actual market.But, at present also
Lack Radix Morinae Bulleyanae nutrition and the systematic research of bait aspect.Food nutrition hinders the fast-growth of Radix Morinae Bulleyanae, some baits the most entirely
Material there is also hygienic issues, carries pathogenic bacterium and causes the generation of disease, even dead, causes huge economic loss.Radix Morinae Bulleyanae is taken the photograph
The bait predominantly Alga Sgrgassi Enerves of food, Thallus Laminariae (Thallus Eckloniae), bean cake etc. are difficult to the material of digested absorption rich in Algin and antinutritional factor etc..
And bait is after beneficial microbe fermentation process, the antinutritional factor in raw material is decomposed or converts, Algin by completely or
Partial digestion, generation is easily searched for food by Radix Morinae Bulleyanae, digests, is absorbed and the little molecular nutrition material of nonhazardous effect.At present, yeast is utilized
Bacterium, bacillus cereus and the existing relevant report of lactic acid bacteria list bacterium fermentation sea cucumber bait, and utilize containing having Algin degradation capability
The rare report of mixed culture solid state fermentation Stichopus japonicus Selenka bait of ocean Pseudoalteromonas, the Stichopus japonicus Selenka bait utilizing this method to prepare can not only
Enough improve the growth performance of Radix Morinae Bulleyanae, improve the economic benefit of enterprise, and the live body probiotic bacteria in fermentation material can play water purification
Effect.The bait of solid fermentation is low compared with liquid fermentation bait water content simultaneously, it is simple to stores and transports；And solid fermentation behaviour
Making simple, low cost, the large-scale production to fermentation bait has important economic worth and realistic meaning.
Summary of the invention
It is an object of the invention to provide a kind of mixing EM bacterium solid fermentation utilized containing ocean Pseudoalteromonas thorn
The method of ginseng bait, thus improve the digestive utilization ratio of bait, improve the growth performance of Radix Morinae Bulleyanae, improve breeding water body environment.
For achieving the above object, we provide the preparation method of a kind of sea cucumber bio bait, by ocean Pseudoalteromonas,
Mixing EM bacterium is inoculated in bait 25-35 DEG C of anaerobic fermentation 3-7 days；Wherein mixing EM bacterium is by bacillus cereus (Bacillus), light
Close antibacterial (Photosynthetic Bacteria, PSB), yeast (Yeast) and lactic acid bacteria (Lactobacillus) composition.
Described bait according to mass percent by 32～40% degumming Kelp Powder, 16～20% Alga Sgrgassi Enerves powder, 16～20%
Sea Calomelas, 6% Semen Maydis powder, 5% breaking cellular wall yeast powder, 4% fermented bean cake, 4% shrimp meal, 3% premix material, 3% fish flour, 2% scallop
Bian Fen, 1% brown sugar composition.
Further, in technique scheme, the preparation method of sea cucumber bio bait specifically includes following steps:
Step a, ocean Pseudoalteromonas are cultivated: be inoculated in seed culture medium by the strain preserved on inclined-plane,
160rpm, 28 DEG C of shaking tables are cultivated 18-20 hour, take seed liquor, receive in fermentation medium with the inoculum concentration of 2%-3%v/v,
160rpm, 28 DEG C of shaking tables are cultivated 24-30 hour.OD600 value is 0.8～1.1.
Step b, mixing EM bacterium are cultivated: mixing EM bacterium includes bacillus cereus, photosynthetic bacteria, yeast and lactic acid bacteria.
Bacillus cereus condition of culture: the strain preserved on inclined-plane is taken 2-3 ring, is inoculated in seed liquor, 180rpm, 30 DEG C
Shaking table is cultivated 18-24 hour, and OD600 value is 0.8～1.1.
Photosynthetic bacteria condition of culture: the strain preserved on inclined-plane is taken 3-4 ring, is inoculated in seed liquor, and 30 DEG C of illumination are quiet
Putting cultivation 72-96 hour, OD600 value is 0.8～1.1.
Yeast condition of culture: the strain preserved on inclined-plane is taken 2-3 ring, is inoculated in seed liquor, 180rpm, and 30 DEG C are shaken
Bed is cultivated 18-24 hour, and OD600 value is 0.8～1.1.
Lactic acid bacteria condition of culture: the strain preserved on inclined-plane is taken 3-5 ring and is inoculated in seed liquor, 37 DEG C of quiescent culture
72-96 hour, OD600 value was 0.8～1.1.
Take the seed liquor of four kinds of strains, respectively with 0.4-0.6%, 0.6-0.9%, 0.4-0.6%, 0.6-0.9%v/v
Inoculum concentration is inoculated in fermentation medium, and at 150-180rpm, under the conditions of 30 DEG C, 500L ferment tank is cultivated 72 hours.
Step c, ocean Pseudoalteromonas fermentation liquid and EM fermented liquid are mixed, according to the inoculum concentration of 2-3% (v/w)
(ocean Pseudoalteromonas fermentation liquid is 1:1.5-3 with the inoculation volume ratio of EM fermented liquid) is inoculated in bait, adjusts water
Dividing content is 38-50%.Load 25kg after mix homogeneously and have in the plastic packaging bag of air check valve, 25-35 DEG C of anaerobic fermentation 3-7
My god.
Step d, when steam occurs in fermentation bait, and hands touches and slightly warms, and bait sends fresh perfume (or spice), has aromatic distillers yeast and acid
During taste, prepared by fermentation bait.
Further, in technique scheme, in step c, in order to ensure that strain is sufficiently mixed with bait, by bacterium solution
After mixing homogeneously with water, then water is mixed with bait, stirs.
Further, in technique scheme, the seed culture medium composition of ocean Pseudoalteromonas in step a: egg
White peptone 0.5g, yeast extract 0.1g, sodium alginate 0.5g, NaCl 3g, water 100mL, adjust pH with 1M NaOH or 1M HCl
Joint is to 7.5；
Ocean Pseudoalteromonas fermentation medium composition: sodium alginate 0.5g, (NH4)SO40.5g, K2HPO40.2g,
MgSO4·7H2O 0.2g, NaCl 2.5g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.5.
Further, in technique scheme, bacillus cereus seed culture medium composition in step b: Carnis Bovis seu Bubali cream 1g, albumen
Peptone 1g, glucose 1g, NaCl 0.5g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.0-7.2.
Photosynthetic bacteria seed culture medium forms: ammonium chloride 1g, KH2PO40.5g, sodium bicarbonate 5g, NaS2O4 1g,NaCl
1g,MgCl20.5g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.5-8.5.
Lactic acid bacteria seed culture medium forms: Carnis Bovis seu Bubali cream 1g, peptone 1g, yeast extract 0.4g, glucose 2g, sucrose 1g, second
Acid sodium 0.5g, ammonium citrate 0.2g, K2HPO40.08g, MgSO4·7H2O 0.02g, MnSO40.005g, precipitated calcium carbonate
0.1g, Tween 80 1ml, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 6.2.
Yeast seed culture medium forms: yeast extract 1g, peptone 2g, glucose 2g, water 100mL, natural pH.
Mixing EM bacteria fermentation culture medium: fulvic acid 0.5g, yeast extract 0.64g, carbamide 0.18g, monosodium glutamate 0.09g, NaCl
0.09g, mushroom powder 0.125g, KH2PO40.31g, lightweight CaCO31.5g, brown sugar 5.6g, natural pH.
Ocean Pseudoalteromonas is from China General Microbiological culture presevation administrative center, numbered
CCREMSDMCC210342。
Bacillus cereus: from Chinese industrial Microbiological Culture Collection administrative center, numbered CICC20037-hay spore
Bacillus.
Photosynthetic bacteria: from China General Microbiological culture presevation administrative center, numbered CCREMSDMCC150016-ball
The red antibacterial of shape.
Yeast: from Chinese agriculture Microbiological Culture Collection administrative center's Chinese microorganism strain preservation storehouse, numbered
ACCC20165-saccharomyces cerevisiae.
Lactic acid bacteria: from Chinese agriculture Microbiological Culture Collection administrative center, numbered ACCC11016-Lactobacillus plantarum.
Invention beneficial effect
1. the present invention uses probiotics fermentation bait, is degraded, slightly by antinutritional factor such as Algins in fermentation raw material
The macromolecular substances such as fiber are decomposed, and generation is easily searched for food by Radix Morinae Bulleyanae, digests, absorbed and the little molecular nutrition thing of nonhazardous effect
Matter.Improve the nutritive value of bait, add the utilization rate of bait, thus save enterprise's production cost, there is bigger warp
Ji meaning and social value.
2. bait after probiotics fermention containing more active probiotic thalline, multiple enzyme, vitamin, metabolite,
Active small peptide, aminoacid, oligosaccharide, antibacterial substance, the immunostimulant factor, somatomedin etc., play promotion Radix Morinae Bulleyanae growth, improve
The effect of Radix Morinae Bulleyanae intestinal microflora, the fragrance ingredient that microbial metabolism simultaneously produces adds its attractant to Radix Morinae Bulleyanae.
3. in fermentation bait rich in active thalline can also play the effect of water purification.Bacillus subtilis can effectively reduce
Ammonia nitrogen, nitrite and hydrogen sulfide levels in apostichopus japonicus culture water body.Lactic acid bacteria and photosynthetic bacteria can be breeding water body and the ends
The organic matter decomposition such as the starch in mud, unnecessary protein, fat, play reduction breeding water body eutrophication, to reduce bed mud rotten
Lose the effect with environmental pollution.
4. bait is during the fermentation, and probiotic bacteria is bred rapidly and become dominant microflora and can suppress harmful bacteria, pathogenic bacterium
Growth, thus reduce the sickness rate of Radix Morinae Bulleyanae, improve the survival rate of Radix Morinae Bulleyanae.
5., compared with liquid fermentation bait, use the mode of solid fermentation, reduce fermentation bait to a great extent
Water content, makes bait be easy to storage and transport, the large-scale production of fermentation bait and promoting is had important economic worth and
Realistic meaning.
Detailed description of the invention
In following embodiment:
Ocean Pseudoalteromonas is from China General Microbiological culture presevation administrative center, numbered
CCREMSDMCC210342。
Bacillus cereus: from Chinese industrial Microbiological Culture Collection administrative center, numbered CICC20037-hay spore
Bacillus.
Photosynthetic bacteria: from China General Microbiological culture presevation administrative center, numbered CCREMSDMCC150016-ball
The red antibacterial of shape.
Yeast: from Chinese agriculture Microbiological Culture Collection administrative center's Chinese microorganism strain preservation storehouse, numbered
ACCC20165-saccharomyces cerevisiae.
Lactic acid bacteria: from Chinese agriculture Microbiological Culture Collection administrative center, numbered ACCC11016-Lactobacillus plantarum.
Ocean Pseudoalteromonas is cultivated:
Being inoculated in seed culture medium by the strain preserved on inclined-plane, 160rpm, 28 DEG C of shaking tables are cultivated 20 hours, take seed
Liquid, receives in fermentation medium with the inoculum concentration of 3%v/v, 160rpm, and 28 DEG C of shaking tables are cultivated 24 hours.OD600 value is 1.0.
The seed culture medium composition of ocean Pseudoalteromonas: peptone 0.5g, yeast extract 0.1g, sodium alginate
0.5g, NaCl 3g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.5；
Ocean Pseudoalteromonas fermentation medium composition: sodium alginate 0.5g, (NH4)2SO40.5g, K2HPO40.2g,
MgSO4·7H2O 0.2g, NaCl 2.5g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.5.
EM bacterium is cultivated in mixing:
Mixing EM bacterium includes bacillus cereus, photosynthetic bacteria, yeast and lactic acid bacteria.
Bacillus cereus condition of culture:
The strain preserved on inclined-plane being taken 2 rings, is inoculated in seed culture medium, 180rpm, 30 DEG C of shaking tables are cultivated 24 hours,
OD600 value is 1.0.
Photosynthetic bacteria condition of culture:
The strain preserved on inclined-plane is taken 3 rings, is inoculated in seed culture medium, 30 DEG C of illumination quiescent culture 72 hours,
OD600 value is 1.0.
Yeast condition of culture:
The strain preserved on inclined-plane being taken 2 rings, is inoculated in seed culture medium, 180rpm, 30 DEG C of shaking tables are cultivated 24 hours,
OD600 value is 1.0.
Lactic acid bacteria condition of culture:
The strain preserved on inclined-plane is taken 3 rings be inoculated in seed culture medium, 37 DEG C of quiescent culture 96 hours, OD600 value
It is 1.0.
Bacillus cereus seed culture medium forms: Carnis Bovis seu Bubali cream 1g, peptone 1g, glucose 1g, NaCl0.5g, water 100mL, uses
1M NaOH or 1M HCl is by pH regulator to 7.0-7.2.
Photosynthetic bacteria seed culture medium forms: ammonium chloride 1g, KH2PO40.5g, sodium bicarbonate 5g, NaS2O4 1g,NaCl
1g,MgCl20.5g, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 7.5-8.5.
Lactic acid bacteria seed culture medium forms: Carnis Bovis seu Bubali cream 1g, peptone 1g, yeast extract 0.4g, glucose 2g, sucrose 1g, second
Acid sodium 0.5g, ammonium citrate 0.2g, K2HPO40.08g, MgSO4·7H2O 0.02g, MnSO40.005g, precipitated calcium carbonate
0.1g, Tween 80 1ml, water 100mL, with 1M NaOH or 1M HCl by pH regulator to 6.2.
Yeast seed culture medium forms: yeast extract 1g, peptone 2g, glucose 2g, water 100mL, natural pH.
Mixing EM bacteria fermentation culture medium: fulvic acid 0.5g, yeast extract 0.64g, carbamide 0.18g, monosodium glutamate 0.09g, NaCl
0.09g, mushroom powder 0.125g, KH2PO40.31g, lightweight CaCO31.5g, brown sugar 5.6g, natural pH.
Embodiment 1
Step a, ocean Pseudoalteromonas are cultivated:
Below the fermentation liquid of ocean Pseudoalteromonas is prepared.
Step b, mixing EM bacterium are cultivated:
Take the bacillus cereus of above-mentioned preparation, photosynthetic bacteria, yeast and the seed liquor of four kinds of strains of lactic acid bacteria, respectively with
0.4%, during the v/v inoculum concentration of 0.6%, 0.4%, 0.6% is inoculated into mixing EM bacteria fermentation culture medium, at 150rpm, 30 DEG C of bars
Under part, 500L ferment tank is cultivated 72 hours.
Step c, ocean Pseudoalteromonas fermentation liquid and EM fermented liquid are mixed, inoculate according to the inoculum concentration of 2%v/w
(ocean Pseudoalteromonas fermentation liquid is 1:1.5 with the inoculative proportion of EM fermented liquid), to (bait formula 1) in bait, adjusts
Moisture is 42%.Load 25kg after mix homogeneously and have in the plastic packaging bag of air check valve 25 DEG C of anaerobic fermentations 7 days.
Step d, when steam occurs in fermentation bait, and hands touches and slightly warms, and bait sends fresh perfume (or spice), has aromatic distillers yeast and acid
During taste, prepared by fermentation bait.
Bait formula 1:
Degumming Kelp Powder 32%, Alga Sgrgassi Enerves powder 20%, sea Calomelas 20%, Semen Maydis powder 6%, breaking cellular wall yeast powder 5%, ferment bean
The dregs of rice 4%, shrimp meal 4%, premix material 3%, fish flour 3%, scallop edge powder 2%, brown sugar 1%.
After bait fermentation, organoleptic indicator is shown in Table 1-1, and physicochemical and microbial index is shown in Table 1-2:
Results of sensory evaluation after the fermentation of table 1-1. embodiment 1 bait 1
Physics and chemistry and microorganism evaluation result after the fermentation of table 1-2 embodiment 1 bait 1
Embodiment 2
Step a, ocean Pseudoalteromonas are cultivated:
Below the fermentation liquid of ocean Pseudoalteromonas is prepared.
Step b, mixing EM bacterium are cultivated:
Take the bacillus cereus of above-mentioned preparation, photosynthetic bacteria, yeast and the seed liquor of four kinds of strains of lactic acid bacteria, respectively with
0.4%, 0.6%, 0.4%, the inoculum concentration of 0.6%v/v be inoculated in mixing EM bacteria fermentation culture medium, at 180rpm, 30 DEG C of bars
Under part, 500L ferment tank is cultivated 80 hours.
Step c, ocean Pseudoalteromonas fermentation liquid and EM fermented liquid are mixed, inoculate according to the inoculum concentration of 2%v/w
(ocean Pseudoalteromonas fermentation liquid is 1:2 with the ratio of EM fermented liquid) arrives in bait (bait formula 2), adjusts moisture and contains
Amount is 45%.Load 25kg after mix homogeneously and have in the plastic packaging bag of air check valve 30 DEG C of anaerobic fermentations 5 days.
Step d, when steam occurs in fermentation bait, and hands touches and slightly warms, and bait sends fresh perfume (or spice), has aromatic distillers yeast and acid
During taste, prepared by fermentation bait.
Bait formula 2:
Degumming Kelp Powder 35%, Alga Sgrgassi Enerves powder 17%, sea Calomelas 20%, Semen Maydis powder 6%, breaking cellular wall yeast powder 5%, ferment bean
The dregs of rice 4%, shrimp meal 4%, premix material 3%, fish flour 3%, scallop edge powder 2%, brown sugar 1%.
Before and after bait fermentation, organoleptic indicator is shown in Table 2-1, and physicochemical and microbial index is shown in Table 2-2:
Results of sensory evaluation after the fermentation of table 2-1. embodiment 2 bait 2
Physics and chemistry and microorganism evaluation result after the fermentation of table 2-2. embodiment 2 bait 2
Embodiment 3
Step a, ocean Pseudoalteromonas are cultivated:
Below the fermentation liquid of ocean Pseudoalteromonas is prepared.
Step b, mixing EM bacterium are cultivated:
Take the bacillus cereus of above-mentioned preparation, photosynthetic bacteria, yeast and the seed liquor of four kinds of strains of lactic acid bacteria, respectively with
0.6%, 0.9%, 0.6%, the inoculum concentration of 0.9%v/v be inoculated in mixing EM bacteria fermentation culture medium, at 180rpm, 30 DEG C of bars
Under part, 500L ferment tank is cultivated 72 hours.
Step c, ocean Pseudoalteromonas and EM bacterium are mixed, inoculate according to the inoculum concentration of 3%v/w that (ocean vacation is alternately
Zymomonas mobilis is 1:2.5 with the ratio of EM bacterium) arrive in bait (bait formula 3), adjusting moisture is 48%.Fill after mix homogeneously
Enter 25kg and have in the plastic packaging bag of air check valve 35 DEG C of anaerobic fermentations 3 days.
Step d, when steam occurs in fermentation bait, and hands touches and slightly warms, and bait sends fresh perfume (or spice), has aromatic distillers yeast and acid
During taste, prepared by fermentation bait.
Bait formula 3:
Degumming Kelp Powder 40%, Alga Sgrgassi Enerves powder 16%, sea Calomelas 16%, Semen Maydis powder 6%, breaking cellular wall yeast powder 5%, ferment bean
The dregs of rice 4%, shrimp meal 4%, premix material 3%, fish flour 3%, scallop edge powder 2%, brown sugar 1%.
Before and after bait fermentation, organoleptic indicator is shown in Table 3-1, and physicochemical and microbial index is shown in Table 3-2:
Results of sensory evaluation after the fermentation of table 3-1. embodiment 3 bait 3
Physics and chemistry and microorganism evaluation result after the fermentation of table 3-2. embodiment 3 bait 3
Comparative example 1
All of step is same as in Example 1, the most except for the difference that ocean Pseudoalteromonas is changed to China Microbiological
CCREMSDMCC210291-ocean Pseudoalteromonas in preservation storehouse, before and after bait fermentation, organoleptic indicator is shown in Table 4-1, physics and chemistry
And microbiological indicator is shown in Table 4-2:
Results of sensory evaluation before and after the fermentation of table 4-1. comparative example 1 bait
Physics and chemistry and microorganism evaluation result before and after the fermentation of table 4-2. comparative example 1 bait
Claims (4)
1. the preparation method of a sea cucumber bio fermentation bait, it is characterised in that: by ocean Pseudoalteromonas
(Pseudoalteromonas), mixing EM bacterium is inoculated in bait 25-35 DEG C of anaerobic fermentation 3-7 days；Wherein mixing EM bacterium is by bud
Spore bacillus (Bacillus), photosynthetic bacteria (Photosynthetic Bacteria, PSB), yeast (Yeast) and lactic acid bacteria
(Lactobacillus) composition.
The preparation method of sea cucumber bio bait the most according to claim 1, it is characterised in that: described bait is according to quality hundred
Mark by 32～40% degumming Kelp Powder, 16～20% Alga Sgrgassi Enerves powder, 16～20% sea Calomelas, 6% Semen Maydis powder, 5% breaking cellular wall ferment
Female powder, 4% fermented bean cake, 4% shrimp meal, 3% premix material, 3% fish flour, 2% scallop edge powder, 1% brown sugar composition.
The preparation method of sea cucumber bio bait the most according to claim 1, it is characterised in that comprise the following steps:
Step a, the cultivation of ocean Pseudoalteromonas: the strain preserved on inclined-plane is inoculated in seed culture medium, 160rpm,
28 DEG C of shaking tables are cultivated 18-20 hour, take seed liquor, receive in fermentation medium with the inoculum concentration of 2%-3%, 160rpm, 28 DEG C
Shaking table is cultivated 24-30 hour, and OD600 value is 0.8～1.1；
Step b, mixing EM bacterium are cultivated: mixing EM bacterium includes bacillus cereus, photosynthetic bacteria, yeast and lactic acid bacteria；
After bacillus cereus cultivation in seed liquor, OD600 value is 0.8～1.1；
After photosynthetic bacteria cultivation in seed liquor, OD600 value is 0.8～1.1；
After yeast cultivation in seed liquor, OD600 value is 0.8～1.1；
After lactic acid bacteria cultivation in seed liquor, OD600 value is 0.8～1.1；
Take the seed liquor of above-mentioned four kinds of postvaccinal strains, respectively with 0.4-0.6%, 0.6-0.9%, 0.4-0.6%, 0.6-
The volume ratio inoculum concentration of 0.9% is inoculated in fermentation medium, at 150-180rpm, under the conditions of 30 DEG C, sends out at 500L fermentation tank
Ferment is cultivated 72 hours；
Step c, ocean Pseudoalteromonas fermentation liquid and EM fermented liquid are mixed, be inoculated into according to the inoculum concentration of 2%-3%
In bait, the adjustment moisture that adds water is 38%-50%；25-35 DEG C of anaerobic fermentation in 25kg plastic packaging bag is loaded after mix homogeneously
3-7 days；Wherein, Pseudoalteromonas fermentation liquid in ocean is 1:1.5～3 with EM fermented liquid inoculation volume ratio；
Step d, when steam occurs in fermentation bait, and hands touches warm, bait sends fresh perfume (or spice), when having aromatic distillers yeast and tart flavour, send out
Prepared by ferment bait.
The preparation method of sea cucumber bio fermentation bait the most according to claim 3, it is characterised in that in step c, by bacterium
After liquid is mixed homogeneously with water, then water is mixed with bait, stirs.
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|---|---|---|---|---|
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| CN108522943A (en) * | 2017-03-06 | 2018-09-14 | 耿胜利 | A kind of preparation method for the bait antistaling agent cultivated using biotechnology |
| CN109090569A (en) * | 2018-08-16 | 2018-12-28 | 贵州省贵三红食品有限公司 | A kind of preparation method of thick chilli sauce |
| CN111543549A (en) * | 2020-06-02 | 2020-08-18 | 烟台绿叶动物保健品有限公司 | Preparation method and application of traditional Chinese medicine microecological preparation |
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101611767A (en) * | 2008-06-27 | 2009-12-30 | 中国科学院沈阳应用生态研究所 | A kind of production method of microbial fermentation bait for sea cucumbers |
| CN103070319A (en) * | 2013-02-22 | 2013-05-01 | 威海金牌生物科技有限公司 | Fermented bait for juvenile sea cucumbers and production method thereof |
| CN103829087A (en) * | 2014-01-17 | 2014-06-04 | 张洁 | Formula and method for preparing sea cucumber culture feed additive |
| CN103989009A (en) * | 2014-05-06 | 2014-08-20 | 威海金牌生物科技有限公司 | Sea cucumber shelf-stable fermented fresh material and production method thereof |
-
2016
- 2016-05-25 CN CN201610356459.4A patent/CN106036198A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101611767A (en) * | 2008-06-27 | 2009-12-30 | 中国科学院沈阳应用生态研究所 | A kind of production method of microbial fermentation bait for sea cucumbers |
| CN103070319A (en) * | 2013-02-22 | 2013-05-01 | 威海金牌生物科技有限公司 | Fermented bait for juvenile sea cucumbers and production method thereof |
| CN103829087A (en) * | 2014-01-17 | 2014-06-04 | 张洁 | Formula and method for preparing sea cucumber culture feed additive |
| CN103989009A (en) * | 2014-05-06 | 2014-08-20 | 威海金牌生物科技有限公司 | Sea cucumber shelf-stable fermented fresh material and production method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 郭航等: ""海藻酸降解菌的筛选及其在刺参发酵饵料制备中的应用"", 《大连工业大学学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522943A (en) * | 2017-03-06 | 2018-09-14 | 耿胜利 | A kind of preparation method for the bait antistaling agent cultivated using biotechnology |
| CN108522943B (en) * | 2017-03-06 | 2021-05-07 | 耿胜利 | Preparation method of bait preservative cultivated by adopting bioengineering technology |
| CN108308374A (en) * | 2018-04-13 | 2018-07-24 | 安徽天邦饲料科技有限公司 | A kind of feed of grass carp having healthcare function based on probiotics resisting stress |
| CN109090569A (en) * | 2018-08-16 | 2018-12-28 | 贵州省贵三红食品有限公司 | A kind of preparation method of thick chilli sauce |
| CN111543549A (en) * | 2020-06-02 | 2020-08-18 | 烟台绿叶动物保健品有限公司 | Preparation method and application of traditional Chinese medicine microecological preparation |
| CN113974024A (en) * | 2021-11-01 | 2022-01-28 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
| CN113974024B (en) * | 2021-11-01 | 2024-03-19 | 中国热带农业科学院热带生物技术研究所 | Sargassum liquid fermentation feed additive, preparation method thereof and application thereof in aquaculture |
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