CN106879821A - The method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian - Google Patents

The method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian Download PDF

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CN106879821A
CN106879821A CN201710020509.6A CN201710020509A CN106879821A CN 106879821 A CN106879821 A CN 106879821A CN 201710020509 A CN201710020509 A CN 201710020509A CN 106879821 A CN106879821 A CN 106879821A
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feed
parts
fermentation
jujun grasses
sheep
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黄吉森
韦明珠
黄光欢
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/517Bifidum
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The present invention relates to the method that a kind of fermentation of Jujun grasses prepares cattle and sheep and Feeds of vegetarian, the parts by weight for preparing each raw material used by the feed are as follows:50~65 parts of Jujun grasses;5~8 parts of zymophyte;6~10 parts of molasses;10~15 parts of grain feed;0.4~1 part of urea phosphate;0.5~0.7 part of salt;12 parts of trace element;0.7~1.0 part of mixed enzyme;8 10 parts of pawpaw skin;7 12 parts of pineapple peel.The present invention carries out fermenting and producing cattle and sheep and Feeds of vegetarian using Jujun grasses, belongs to forage and recycles project, and waste residue, environmental protection are not discharged externally;By fermentation, effectively feed is decomposed, the small-molecule substances such as glucose, amino acid that animal easily digests and assimilates are converted into, improve the nutritive value of feed and utilize conversion ratio, during the fermentation simultaneously, effectively suppress harmful miscellaneous bacteria and the pathogenic microorganism adhered on feed, feed quality is improved, the feed nutrition obtained after fermentation enriches, and good palatability.

Description

The method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian
Technical field
The present invention relates to breeding feed technical field, and in particular to a kind of Jujun grasses fermentation prepares cattle and sheep and plant-eating animal is raised The method of material.
Background technology
At present, Juncao technology has propagated to more than 100, whole world country.But Jujun grasses all fail utilization very well all the time. Fuel or paper making raw material typically are only used as, a large amount of wastes of resource are caused.
Jujun grasses, alias:Imperatoria, hybrid giant napier, huge napier grass, are perennial, the upright grass for growing thickly, and are by brother's human relations A kind of high yield, high-quality than the introduction of sub- state cradle type forage grass.Jujun grasses are once planted, and can continuously be gathered in 8~10 years, its root system Prosperity, tillering ability is strong.Individual plant cultivate, then can 80~100 plants of tiller, Second Year continue tiller up to 20~40 plants, At most can reach more than 120 plants.Earth's surface top erect plants are grown thickly, and profile and growth are similar to sugarcane.Plant reaches as high as 5 meters More than, stem slightly up to 3 centimetres, referred to as grass in king.Guangxi is one of Chinese big province of popularizing planting Jujun grasses, and its cultivated area is Up to nearly 50,000 mu, per mu yield surpasses 20 tons, often produces Jujun grasses per year and is about more than 1,000,000 tons, and resource is enriched very much, and yield is considerable, Jujun grasses its crude fiber contents is up to 18-25%, its hay thick protein 18.46%, protamine matter 16.86%, crude fat 1.74%, total sugar 8.3%, coarse ash, with wide, the big and low-cost feature of quantity of originating, contains Plant Light more than 9% The cooperation energy of more than half for being accumulated, as the feed resource of noncompetitive, as long as rational processing can be carried out adjusting System, improves the intake of its digestible energy, particularly for feeding the ruminant domestic animals such as ox, sheep, remains able to turn into high-quality feed source. How in good taste Jujun grasses rich in nutrition content, juice is, is the splendid feed of various food grass livestocks, poultry and fish.According to relevant section Grind department's measure:Its is nutritious, includes 17 kinds of amino acid and various vitamins, fresh grass thick protein 4.6%, protamine matter 3%, crude fat 1.74%, sugar more than 3%.Hay calorie value be 3600 kilocalories/kilogram.Therefore, according to Jujun grasses (emperor bamboo Grass) animal feed is prepared, resource can not only be made to be fully used, moreover it is possible to expand the raw material sources of feed, reduce and raise Material cost, has a extensive future.At present, in terms of animal feed is prepared using Jujun grasses (hybrid giant napier), the text of correlation is retrieved Offer as:
1st, Chinese patent, title:A kind of hybrid giant napier is the method that primary raw material prepares pork pig feed, application(Patent)Number: 201210072981.1, applicant:He Han, summary:The invention is related to the technology of preparing of a boar food, belongs to stalk depth Manufacture field, and in particular to a kind of with the method that hybrid giant napier is primary raw material preparation pork pig feed.Specifically include:Come with raw material Extensive, the cheap hybrid giant napier in source is primary raw material, is auxiliary material with corn flour, analysis for soybean powder and fish meal, is fermented with EM beneficial bacteriums Form that a kind of thing favorable rates is honest and clean, comprehensive nutrition pork pig feed.The pig feed prepared by the present invention, comprehensive nutrition feeds pork pig When can as complete feed, without add hormone and auxin;Beneficial microbe secretion activity material can promote pork pig in feed Human body significantly increases immunity, reduces veterinary drug and uses even without veterinary drug, produces the harmless high-quality pork pig product of green.
2nd, Chinese patent, title:A kind of hybrid giant napier pig feed and preparation method thereof, application(Patent)Number: 201510137898.1, applicant:Hunan Xintai City and green agriculture Group Co., Ltd, summary:A kind of emperor of the disclosure of the invention Bamboo grass pig feed, the raw material and its quality proportioning of the hybrid giant napier pig feed is as follows:Hybrid giant napier:77.84 %;Rice bran: 4.67%-18.68%;Rapeseed cake:3.11%-17.12%;Salt:0.31%;Leavening and degradation agent:0.06%.It is of the invention real The hybrid giant napier pig feed of example offer is applied, solve makes pig as pig feed usually using grain, import transgenosis dregs of beans etc. Relatively costly problem, efficiently solves the source of feed, greatly reduces feed cost, for production Green Meat has established thing Matter basis.
3rd, Chinese patent, title:A kind of forage for turkey with hybrid giant napier as raw material, application number:201610445992.8, Applicant:Binyang County Gu La towns new people's featured agriculture cultivation farmer specialized cooperative society, summary:The invention is related to one kind with emperor bamboo Grass is the forage for turkey of raw material, is made up of the raw material of following mass percent:Hybrid giant napier tunning 40-55%, corn flour 10- 15%, flour or ground rice 10-15%, peanut press pulp 3-5%, fish meal 3-5%, calcium powder 0.5-1%, rice bran or wheat bran are supplied 100%.Described hybrid giant napier tunning is obtained by following methods:Fresh hybrid giant napier is crushed;The 7-9% chlorinations that will be modulated On sodium salt Water spray hybrid giant napier after being pulverized, reinstall in fermentation tank, spread one layer of salt on hybrid giant napier, sealed fermenter, Fermented 5-15 days under the conditions of being placed in 25-35 DEG C.Organoleptic properties of the present invention are good, and palatability is good, are of high nutritive value;Turkey absorbs Effect is good, and stomach is good, additionally it is possible to save feed, reduces feed-weight ratio;Fermentation period is short, long shelf-life.
All it is to use hybrid giant napier that feed is prepared for fermenting raw materials in above-mentioned document, but the weak point for existing is to prepare Feed nutrition it is not comprehensive enough, trace element required for having lacked animal etc., it is impossible to fully meet the growth needs of animal.And And at present in terms of the exploitation of hybrid giant napier, the rarely found text having in terms of using hybrid giant napier to be prepared cattle and sheep feed for raw material Offer report.
The content of the invention
It is an object of the invention to provide the method that a kind of fermentation of Jujun grasses prepares cattle and sheep and Feeds of vegetarian, using huge bacterium Grass carries out fermentation and prepares cattle and sheep and Feeds of vegetarian, and processing method is simple, low cost, by fermentation after huge bacterium Grass, crude fibre can be degraded, and crude protein can be improved, and its nutritive value has obtained further improving, and is prepared after fermentation Cattle and sheep and Feeds of vegetarian comprehensive nutrition, have light wine flavour, good palatability, improve the food of cattle and sheep and plant-eating animal It is intended to.
In order to realize the purpose of the present invention, the present invention is adopted the following technical scheme that:
A kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, comprises the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, is then well mixed with molasses, obtain mixed solution, then by step(1)In Jujun grasses and mixed solution mix, and add grain feed, nonprotein nitrogen, salt and trace element, are stirred with mixer, Fermentation material is obtained, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, ferment 8-10 at being placed in 35~40 DEG C My god;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 40- At 45 DEG C, sealed fermenting is obtained feed after 2-3 days.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, described zymophyte be lactic acid bacteria, Clothing bacillus, S. cervisiae and bifidobacterium bifidum are according to 3:1:2:What 1 part by weight was mixed to get.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, the step(2)It is middle to enter zymophyte The composition of the culture medium used by row Amplification Culture is:5-7% tapiocas, 1%-2% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4·3H2O ;The compound dimensions of 0.1-0.3% Raw element;10% carrot juice;Cultural method is:Strain is inoculated in seed fermentation tank, seed fermentation tank fermentation condition:Temperature 30 DEG C, pH 7.0-8.0, inoculum concentration 7%, mixing speed 240r/min, throughput is 6L/min, fermentation time 55h.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, described mixed enzyme is aspergillus oryzae, black Aspergillus, amylopsin and Trichoderma viride are according to 2:1:2:What 1.5 part by weight was mixed to get.
The method that described Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, prepares each raw material used by the feed Parts by weight it is as follows:50~65 parts of Jujun grasses;5~8 parts of zymophyte;6~10 parts of molasses;10~15 parts of grain feed;Phosphoric acid 0.4~1 part of urea;0.5~0.7 part of salt;It is micro- 1-2 parts;0.7~1.0 part of mixed enzyme;8-10 parts of pawpaw skin;Pineapple peel 7-12 parts;Described grain feed includes wheat bran, corn, potato powder, rice bran and peanut meal;Described trace element be by manganese, Zinc, iron and calcium composition, manganese are selected from Mn-Gly, and zinc is selected from zinc oxide, and iron is selected from ironic citrate, and calcium is selected from calcium iodate.
Molasses used by the present invention, it is remaining uncrystallizable after being white granulated sugar production process crystallization of sucrose, but still containing compared with The liquid residue of polysaccharide.The main component of molasses is carbohydrate, the cane molasses about 24%-36% containing sucrose, other sugar about 12%- 24%;Molasses contain a small amount of thick protein, and generally 3%-6% belongs to nonprotein nitrogen class, such as ammonia, acid amides and nitrate more, and Amino-acid nitrogen only accounts for 38%-50%, and nonessential amino acid such as asparatate, content of glutamic acid is more, therefore protein is given birth to Thing value is relatively low.Present invention addition molasses have two effects, and one is to increase carbon source and nitrogen source in fermentation process, improves fermentation effect Really, two be add feed in, improve feed palatability.
In zymophyte of the present invention, lactic acid bacteria (lactic acid bacteria, LAB) is that a class can be using can Fermentable carbohydrates produce the common name of the bacterium of a large amount of lactic acid.Lactic acid bacteria is added in feed, Laying chicks survival rate can be improved And daily gain, young dog body weight is dramatically increased after can making ablactation, therefore significantly improves efficiency of feed utilization.Fermented using lactic acid bacteria Feed can degrade the ANFs in feed(Soybean antigen, trypsin ihhibitor etc.)With poisonous material(As guarded against Sub- acid), the difficult nutrient by animal use of release, such as phytate phosphorus;Chelated microelement(Calcium carbonate is such as become calcium lactate), Promote the absorption of trace element;Degraded crude fibre is into monose.Lactic acid bacteria grows to some fungies has inhibitory action with product poison, while Also there is suction-operated to already present toxin.Lactic acid bacteria has suction-operated to aflatoxin, while it can also be to yellow bent Mould toxin is degraded, and reaches detoxification efficiency.And there is special fermenting aroma by the feed after lactobacillus-fermented, contain Abundant lactic acid, phagostimulating effect is good, and feed intake is high.The present invention carries out fermentation Jujun grasses using lactic acid bacteria as one of fermented bacterium Feed is prepared, ferment effect can not only be improved, and lactic acid bacteria can also be chelated with trace element is added, and promote animal to micro- The absorption of secondary element.
The cellular morphology of bacillus licheniformis and arrangement are in shaft-like, Dan Sheng, and Classification system is:Bacillus Lichenif0rmis, the adjustable flora imbalance of bacillus licheniformis reaches therapeutic purposes, and body can be promoted to produce antibacterial activity thing Matter, killing pathogenic bacteria.Resistant activity material can be produced, and with unique biology take oxygen mechanism, the life of pathogenic bacteria can be suppressed Breeding long.Promote the growth of normal physiological anaerobic bacteria in enteron aisle, adjust intestinal bacilli illness, recover function of intestinal canal;Can produce Resistant activity material, and with unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be suppressed.
Bifidobacterium bifidum (Bifidobacterium bifidum) is a kind of probiotics in humans and animals enteron aisle, both The various biological functions such as intestinal eubiosis, antibacterial, enhancing immunologic function, antitumor action are safeguarded, in prevention and treatment Disease and the aspect that promotes health play an important role.Bifidobacterium can produce lactic acid and vinegar after the fermentation of human body enteral Acid, can improve the utilization rate of calcium, phosphorus, iron, promote the absorption of iron and vitamin D.The propagation of Bifidobacterium can improve enteron aisle ring Border, the growth for suppressing intestinal harmful bacillus, raising body immunity, play an important role to Constipation, anti-disease, anti-aging.
Saccharomyces cerevisiae (Saccharomyces cerevisiae), also known as Saccharomyces cerevisiae or budding yeast.In poultry feed Middle to add saccharomycete or its culture, kind, the production performance of poultry in can improving improve excrement bacterium colony, have a net increase of strong body and exempt from Epidemic disease power.And saccharomyces cerevisiae can produce the edible protein of people and animals using molasses, wood hydrolysis liquid etc., can adjust animal intestine Road microecological balance, improves feed digestibility, strengthens animal body immunity.Saccharomyces cerevisiae can also utilize cellulose raw material Production nutrition increases rich fermented feed, and yeast can not only greatly improve the protein level of feedstuff, and saccharomycete is also containing rich Rich enzyme, such as pepsin, amylase, digestibility are high, and general digestibility is up to 80%~90%.Yeast fermented feed can promote Enter microbial reproduction, improve digestibility, promote weightening, improve the price of deed.And, yeast can strengthen the digestion to nutriment Utilize, promote growth of animals or poultry, promote the ability of its appetite, enhancing resist the disease and resisting stress.
In mixed enzyme of the present invention, aspergillus oryzae (Aspergillus oryzae) is a kind of aerobic fungi, is one Class produces the bacterial strain of complex enzyme, in addition to protease is produced, can also produce amylase, carbohydrase, cellulase, phytase etc..In amylase In the presence of, the straight chain in raw material, amylopectin are degraded to dextrin and various low molecule carbohydrates, such as maltose, glucose; In the presence of protease, stodgy macro-molecular protein is degraded to peptone, polypeptide and various amino acid, Er Qieke So that the difficult mass degradation for absorbing such as crude fibre, phytic acid in auxiliary material, improves nutritive value, health-care efficacy and digestibility.By meter Qu It is mould to carry out fermented feed as one of mould, can not only improve the efficiency of fermentation, and also feed has one light after making fermentation Wine flavour, animal more likes to eat.
Aspergillus niger (Aspergillus niger), Deuteromycotina, Hyphomycetes, hyphomycetales, Moniliaceae, aspergillus A Common Species in fungi.Aspergillus niger is used for fermented feed, during the fermentation, the life that aspergillus niger passes through microorganism itself Life activity, makes poisonous and harmful substance contained in feed be degraded and remove, so as to substantially increase the security of feed;Black song The mould macromolecular carbohydrate that can be decomposed in feed is monose and oligosaccharides, and generates various organic acids, vitamin, biology enzyme, unknown life The factor long, substantially increases the trophic level and digestibility of fermented feed, and aspergillus niger is used for into fermented feed, can make feed Removing toxic substances detoxification, greatly improves the security of feed, and light fragrance can be produced during fermentation, dynamic so as to increased The palatability of thing;Various organic acids, vitamin, biology enzyme, amino acid and other various unknown lifes can also be generated in fermentation process The factor long, substantially increases the trophic level and digestive utilization ratio of fermented feed;Improve animal resistance, reduce feed into This.
Amylopsin(pancreaticamylase)It, by a kind of hydrolase of pancreatic secretion, is to act on soluble shallow lake to be α-Isosorbide-5-Nitrae-the glucan such as powder, amylose, glycogen, the enzyme of hydrolyzing alpha-Isosorbide-5-Nitrae-glycosidic bond.Belong to one kind of AMS.Pancreas starch Enzyme can be by Starch Hydrolysis into maltose and glucose.The straight chain in raw material, amylopectin can be degraded to paste during the fermentation Smart and various low molecule carbohydrates, such as maltose, glucose, improve the trophic level and digestibility of fermented feed, increase Add the palatability of animal, promoted to digest and assimilate, improve efficiency of feed utilization.
Trichoderma viride, mycelia is very thin colourless, and tool separates, multi-branched;Classification system is Trich0derma viride, certainly Right boundary is widely distributed, often saprophytic on timber, seed and plant residue.Trichoderma viride is institute cellulase-producing activity highest bacterium One of strain, produced cellulase has degradation to crop, and effect is very good, and Trichoderma viride can produce various with biology The enzyme system of activity, such as:Cellulase etc..Trichoderma has stronger decomposition of cellulose ability, and Trichoderma viride usually can produce height The cellulase of activity, the capacity of decomposition to cellulose is very strong.And contain substantial amounts of cellulose in Jujun grasses, use Trichoderma viride Bacterium, can degrade bagasse fibre during the fermentation.
The mixed enzyme that the present invention is used is aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride according to 2:1:2:1.5 weight Amount ratio is mixed to get.Good fermentation can be played to Jujun grasses using the mixed enzyme, and use the mixed enzyme pair Jujun grasses carry out secondary fermentation, can greatly improve the efficiency of fermentation.
The zymophyte that the present invention is used plays good fermentation to Jujun grasses, and substantial amounts of fiber is contained in Jujun grasses Element, the microorganism in zymophyte increases rich fermented feed during growth and breeding using cellulose raw material production nutrition, in height In the presence of effect factoring, the crude fibre in Jujun grasses, lignin, the effect by biological biochemistry can not inhale livestock and poultry The polymeric carbohydrate of receipts changes into the absorbable low point of carbohydrate for utilizing;Multiple beneficial flora energy in zymophyte It is a large amount of to draw organic nitrogen, inorganic nitrogen that livestock and poultry are difficult by, it is allowed to change into nutrition bacterium protein higher;Leavening In probioticses can produce a large amount of protease, cellulolytic enzyme etc. in fermentation, can be complicated in degrading plant feed Organic matter, so as to promote to digest and assimilate, improves efficiency of feed utilization;Profitable strain enhances immunity in livestock and poultry stomach, reduces Disease occurs.Microbe conversion treatment is carried out to Jujun grasses by using zymophyte, Jujun grasses crude fiber content is than azymic at double Reduce, crude protein content is significantly improved than azymic.
And, Jujun grasses are added mixed enzyme and carry out secondary fermentation after carrying out one time fermentation using zymophyte, also, are passed through The further cellulose decomposed in Jujun grasses of the secondary fermentation of mixed enzyme, so that provide can absorbed nutriment;Carry significantly The trophic level and digestibility of fermented feed high, increased the palatability of animal;And in the feed obtained after fermentation After the mixed enzyme for containing is used to feed ox or sheep, into the enteron aisle of livestock and poultry, stomach and intestine function can be improved, effectively in suppression animal and bird intestines The breeding of pathogen, improves immunity of organisms, reduces morbidity and mortality.
The beneficial effect comprise that:
1st, Jujun grasses all fail utilization very well all the time, are intended only as fuel or paper making raw material, cause a large amount of waves of resource Take.The present invention carries out fermenting and producing cattle and sheep and Feeds of vegetarian using Jujun grasses, belongs to forage and recycles project, does not arrange externally Put waste residue, environmental protection;Contain substantial amounts of cellulose in Jujun grasses, during the fermentation, the microorganism in zymophyte is in growth Produce nutrition using cellulose raw material in reproductive process and increase rich fermented feed, by fermentation after Jujun grasses, crude fibre can To degrade, crude protein can be improved, and its nutritive value has obtained further improving.
2nd, the cattle and sheep that prepare of the present invention and Feeds of vegetarian do not contain chemobiotic class medicine, safety and environmental protection, Fermentation being carried out using Jujun grasses and preparing cattle and sheep and Feeds of vegetarian, a large amount of celluloses contained in Jujun grasses are decomposed, Crude fiber content reduction, the crude protein content of feed after fermentation is improved, and by fermentation, effectively by feed point Solve, be converted into the animal easily small-molecule substance such as glucose, amino acid for digesting and assimilating, thus improve feed nutritive value and Using conversion ratio, while during the fermentation, effectively suppressing harmful miscellaneous bacteria and the pathogenic microorganism adhered on feed, improve and raise Item matter.
3rd, the method that Jujun grasses fermentation of the present invention prepares cattle and sheep and Feeds of vegetarian, processing method is simple, passes through The food that the feed nutrition material prepared after secondary fermentation enriches, there is light wine flavour, improve cattle and sheep and plant-eating animal It is intended to.Zymophyte and mixed enzyme are with the addition of during the fermentation, and the beneficial microbe in fermented feed is raised with fermentation in animal body During material, substantial amounts of animal growth, digestive ferment, organic acid and somatomedin necessary to physiological activity, side are produced Help all drop materials of the body to being searched for food to digest and assimilate, promote internal organic synthesis and normal physiological metabolism, So as to enhancing development, daily gain and the price of deed are improved.Contained beneficial microbe in the feed that the present invention is prepared Digestibility and utilization of the cattle and sheep to nutriment can be strengthened, promote the growth of cattle and sheep, improve stomach and intestine function, promote its appetite, effectively Suppress the breeding of pathogen in animal and bird intestines, enhance immunity, improve the ability of resist the disease, reduce disease and occur.
4th, zymophyte of the present invention first passes through the Amplification Culture of strain, substantially increases the quantity of strain, improves Fermentation efficiency, and culture medium used by strain Amplification Culture is also a kind of good nutritional ingredient, is made for fermenting together Standby Feed Energy improves the nutritional ingredient of feed.
5th, it is of the invention except the Jujun grasses for using additionally use the raw materials, molasses such as molasses, pawpaw skin, pineapple peel in addition to raw material Containing sugar content higher, good palatability contains papain, bromelain is contained in pineapple peel in pawpaw skin, can With further the carrying out for promoting and fermenting, and pawpaw skin and pineapple peel are also in itself good feedstuff, by itself and huge bacterium Grass ferments together, not only turns waste into wealth, moreover it is possible to greatly enriched the nutrition of feed.
6th, the raw material of fermented feed of the present invention is also added into trace element, can promote the animal growth process such as cattle and sheep In for micro substance demand, meanwhile, trace element can in time supplement that trace element in various main materials is unstable to be lacked again Fall into, effectively improve utilization rate of fertilizer, safe, organic, environmental protection;And addition trace element can also be chelated with lactic acid bacteria, be promoted Enter absorption of the animal to trace element.
7th, the present invention utilizes Jujun grasses fermenting and producing cattle and sheep and Feeds of vegetarian, realizes Jujun grasses low cost, nothing two The comprehensive utilization of secondary pollution, does not produce " three wastes " in production process, be that the comprehensive utilization of Jujun grasses opens a new way again Footpath, and each raw material sources used by the present invention are extensive, cheap, by the feed that its fermentation processing is the animals such as cattle and sheep, Turn waste into wealth, greatly reduce production cost, not only increase the economic benefit of enterprise, also for animal husbandry provides substantial amounts of feeding Material resource, improves economic benefit and social benefit, to grass cultivation, animal husbandry, feedstuff industry and environment sustainable development aspect All it is extremely important.
Specific embodiment
Embodiment 1
A kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, comprises the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, described zymophyte is lactic acid bacteria, bacillus licheniformis, S. cervisiae, not tally Bifidobacterium is according to 3:1:2:What 1 part by weight was mixed to get;(The composition of the culture medium used by Amplification Culture is:5% cassava Starch, 1% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4·3H2O ;0.1% B B-complex;10% carrot juice;Cultural method is:Strain is inoculated in seed fermentation In tank, seed fermentation tank fermentation condition:30 DEG C of temperature, pH 7.0, inoculum concentration 7%, mixing speed 240r/min, throughput is 6L/ Min, fermentation time 55h.)After Amplification Culture, it is well mixed with molasses, obtains mixed solution, then by step(1)In huge bacterium Grass and mixed solution mixing, and peanut meal, nonprotein nitrogen, salt and trace element are added, stirred with mixer, sent out Ferment material, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, fermented 10 days at being placed in 35 DEG C;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 40 DEG C Under, sealed fermenting is obtained feed after 3 days.Described mixed enzyme be aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride according to 2:1:2:What 1.5 part by weight was mixed to get.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, prepares each raw material used by the feed Parts by weight it is as follows:50 parts of Jujun grasses;5 parts of zymophyte;6 parts of molasses;10 parts of peanut meal;0.4 part of urea phosphate;0.5 part of salt; 1 part of trace element;0.7 part of mixed enzyme;8 parts of pawpaw skin;7 parts of pineapple peel;Described trace element is by manganese, zinc, iron and calcium group Into manganese is selected from Mn-Gly, and zinc is selected from zinc oxide, and iron is selected from ironic citrate, and calcium is selected from calcium iodate.
Embodiment 2
A kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, comprises the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, described zymophyte is lactic acid bacteria, bacillus licheniformis, S. cervisiae, not tally Bifidobacterium is according to 3:1:2:What 1 part by weight was mixed to get;(The composition of the culture medium used by Amplification Culture is:6% cassava Starch, 1.5% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4·3H2O ;0.2% B B-complex;10% carrot juice;Cultural method is:Strain is inoculated in seed hair In fermentation tank, seed fermentation tank fermentation condition:30 DEG C of temperature, pH 8.0, inoculum concentration 7%, mixing speed 240r/min, throughput is 6L/min, fermentation time 55h.)After Amplification Culture, it is well mixed with molasses, obtains mixed solution, then by step(1)In Jujun grasses and mixed solution mix, and add grain feed, nonprotein nitrogen, salt and trace element, are stirred with mixer, Fermentation material is obtained, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, fermented 8 days at being placed in 40 DEG C;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 45 DEG C Under, sealed fermenting is obtained feed after 2 days.Described mixed enzyme be aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride according to 2:1:2:What 1.5 part by weight was mixed to get.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, prepares each raw material used by the feed Parts by weight it is as follows:60 parts of Jujun grasses;6 parts of zymophyte;8 parts of molasses;13 parts of grain feed;0.7 part of urea phosphate;Salt 0.6 Part;1.5 parts of trace element;0.8 part of mixed enzyme;9 parts of pawpaw skin;9 parts of pineapple peel;Described grain feed is wheat bran and jade Rice;Described trace element is made up of manganese, zinc, iron and calcium, and manganese is selected from Mn-Gly, and zinc is selected from zinc oxide, and iron is selected from lemon Sour iron, calcium is selected from calcium iodate.
Embodiment 3
A kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, comprises the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, described zymophyte is lactic acid bacteria, bacillus licheniformis, S. cervisiae, not tally Bifidobacterium is according to 3:1:2:What 1 part by weight was mixed to get;(The composition of the culture medium used by Amplification Culture is:7% cassava Starch, 2% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4·3H2O ;0.3% B B-complex;10% carrot juice;Cultural method is:Strain is inoculated in seed fermentation In tank, seed fermentation tank fermentation condition:30 DEG C of temperature, pH 7.0-8.0, inoculum concentration 7%, mixing speed 240r/min, throughput It is 6L/min, fermentation time 55h.)After Amplification Culture, it is well mixed with molasses, obtains mixed solution, then by step(1)In Jujun grasses and mixed solution mixing, and add grain feed, nonprotein nitrogen, salt and trace element, stir equal with mixer It is even, fermentation material is obtained, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, fermented 9 days at being placed in 38 DEG C;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 42 DEG C Under, sealed fermenting is obtained feed after 2.5 days.Described mixed enzyme be aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride by According to 2:1:2:What 1.5 part by weight was mixed to get.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, prepares each raw material used by the feed Parts by weight it is as follows:65 parts of Jujun grasses;8 parts of zymophyte;10 parts of molasses;15 parts of grain feed;1 part of urea phosphate;Salt 0.7 Part;2 parts of trace element;1.0 parts of mixed enzyme;10 parts of pawpaw skin;12 parts of pineapple peel;Described grain feed be potato powder, rice bran and Peanut meal;Described trace element is made up of manganese, zinc, iron and calcium, and manganese is selected from Mn-Gly, and zinc is selected from zinc oxide, and iron is selected from Ironic citrate, calcium is selected from calcium iodate.
Embodiment 4:
A kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, comprises the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, described zymophyte is lactic acid bacteria, bacillus licheniformis, S. cervisiae, not tally Bifidobacterium is according to 3:1:2:What 1 part by weight was mixed to get;(The composition of the culture medium used by Amplification Culture is:6% cassava Starch, 2% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4·3H2O;0.15% B B-complex;10% carrot juice;Cultural method is:Strain is inoculated in seed fermentation In tank, seed fermentation tank fermentation condition:30 DEG C of temperature, pH 7.0-8.0, inoculum concentration 7%, mixing speed 240r/min, throughput It is 6L/min, fermentation time 55h.)After Amplification Culture, it is well mixed with molasses, obtains mixed solution, then by step(1)In Jujun grasses and mixed solution mixing, and add grain feed, nonprotein nitrogen, salt and trace element, stir equal with mixer It is even, fermentation material is obtained, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, fermented 9 days at being placed in 40 DEG C;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 40 DEG C Under, sealed fermenting is obtained feed after 2 days.Described mixed enzyme be aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride according to 2:1:2:What 1.5 part by weight was mixed to get.
The method that above-mentioned Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, prepares each raw material used by the feed Parts by weight it is as follows:60 parts of Jujun grasses;7 parts of zymophyte;9 parts of molasses;11 parts of grain feed;0.8 part of urea phosphate;Salt 0.6 Part;1.6 parts of trace element;0.8 part of mixed enzyme;10 parts of pawpaw skin;8 parts of pineapple peel;Described grain feed includes wheat bran, jade Rice, potato powder, rice bran and peanut meal;Described trace element is made up of manganese, zinc, iron and calcium, and manganese is selected from Mn-Gly, and zinc is selected from Zinc oxide, iron is selected from ironic citrate, and calcium is selected from calcium iodate.
Feeding experiment:
(1) test material
Test group:From feed obtained in embodiment:
Control group:Fed with conventional feed, be made up of the raw material of following parts by weight:20 parts of corn straw silage, maize flour 6 Jin, 1.0 jin of premix, 4 parts of dregs of beans, 3 parts of molasses, 10 parts of straw.
For examination sheep:200 sheep are extracted, every body weight of sheep is 30 jin or so.
(2) test method
200 bellwethers are randomly divided into 2 groups, every group of 100 bellwethers.
Test group and control group measure original body mass respectively, and experiment terminates measurement latter stage body weight, record feed consumption, hair Sick rate and calculating average daily gain, are free choice feeding, 75 days experimental periods.
(3)Experimental result is as shown in table 1 below:
Result of the test is shown, is fermented using Jujun grasses of the present invention and prepare the feeding that the method for cattle and sheep and Feeds of vegetarian is prepared Expect for feeding sheep, compared with the conventional feed used by sheep is usually fed, the sheep speed of weight increment for eating feed of the present invention is very fast, the incidence of disease It is low.Fed using feed of the present invention, can not only be reached the preferable daily gain effect of sheep, and can guarantee that the health of sheep is raw Long, sheep likes the feed that the edible present invention is developed, and the mutton quality for obtaining might as well.

Claims (5)

1. a kind of method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian, it is characterised in that comprise the following steps:
(1)The Jujun grasses come are transported back by after pulverization process from planting site, it is stand-by;
(2)Zymophyte is enlarged culture, is then well mixed with molasses, obtain mixed solution, then by step(1)In Jujun grasses and mixed solution mix, and add grain feed, nonprotein nitrogen, salt and trace element, are stirred with mixer, Fermentation material is obtained, its water content is 50~70%;
(3)By step(2)In the fermentation material that obtains be fitted into packaging bag, sack is tamping, ferment 8-10 at being placed in 35~40 DEG C My god;
(4)In step(3)Add pawpaw skin and pineapple peel after mixed enzyme and chopping well mixed in the material for obtaining, in 40- At 45 DEG C, sealed fermenting is obtained feed after 2-3 days.
2. the method that Jujun grasses fermentation according to claim 1 prepares cattle and sheep and Feeds of vegetarian, it is characterised in that institute The zymophyte stated is lactic acid bacteria, bacillus licheniformis, S. cervisiae and bifidobacterium bifidum according to 3:1:2:1 part by weight It is mixed to get.
3. the method that Jujun grasses fermentation according to claim 1 prepares cattle and sheep and Feeds of vegetarian, it is characterised in that institute State step(2)The middle composition of culture medium that is enlarged zymophyte used by culture is:5-7% tapiocas, 1%-2% glucose, 0.1% urea, soy peptone 0.3%, 0.008%MgSO4·7H2O, 0.005%FeSO4·7H2O , 0.03% K2HPO4· 3H2O ;0.1-0.3% B B-complexes;10% carrot juice;Cultural method is:Strain is inoculated in seed fermentation tank, seed Ferment tank condition:30 DEG C of temperature, pH 7.0-8.0, inoculum concentration 7%, mixing speed 240r/min, throughput is 6L/min, Fermentation time 55h.
4. the method that Jujun grasses fermentation according to claim 1 prepares cattle and sheep and Feeds of vegetarian, it is characterised in that institute The mixed enzyme stated is aspergillus oryzae, aspergillus niger, amylopsin and Trichoderma viride according to 2:1:2:1.5 part by weight is mixed to get 's.
5. the method that Jujun grasses fermentation according to claim 1 prepares cattle and sheep and Feeds of vegetarian, it is characterised in that system The parts by weight of each raw material used by the standby feed are as follows:50~65 parts of Jujun grasses;5~8 parts of zymophyte;Molasses 6~10 Part;10~15 parts of grain feed;0.4~1 part of urea phosphate;0.5~0.7 part of salt;It is micro- 1-2 parts;Mixed enzyme 0.7~ 1.0 parts;8-10 parts of pawpaw skin;Pineapple peel 7-12 parts;Described grain feed includes wheat bran, corn, potato powder, rice bran and peanut The dregs of rice;Described trace element is made up of manganese, zinc, iron and calcium, and manganese is selected from Mn-Gly, and zinc is selected from zinc oxide, and iron is selected from lemon Sour iron, calcium is selected from calcium iodate.
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CN109043140A (en) * 2018-09-06 2018-12-21 南充市嘉陵区黄家楼优土生态种养专业合作社 A kind of lactic acid bacteria Jujun grasses formula forage being effectively improved herbivore mouthfeel
CN110089619A (en) * 2019-06-03 2019-08-06 潘国雄 A kind of Chinese medicine bacteria preparation fermented feed and preparation method thereof
CN110506835A (en) * 2019-09-24 2019-11-29 覃小柳 The method for preparing animal feed using microbial fermentation Jujun grasses
CN110537624A (en) * 2019-09-24 2019-12-06 谢新佑 preparation method of Jujun grass fermented feed
CN111387341A (en) * 2020-03-30 2020-07-10 广西大学 Method for processing wangcao and bacterium enzyme synergistic fermentation composition thereof
CN111772038A (en) * 2020-07-31 2020-10-16 宝绿康(北京)生物科技研究院 Antibiotic-free feed containing juncao and protein mulberry or broussonetia papyrifera, preparation method and application
CN113424902A (en) * 2021-04-30 2021-09-24 周川 Beef cattle feed capable of improving immunity and preparation method thereof
CN114052127A (en) * 2021-11-10 2022-02-18 贵州省草业研究所 Complete feed for improving growth rate of black goat lambs and preparation method thereof
CN114365794A (en) * 2022-01-18 2022-04-19 北京平安幸福农业发展有限公司 Functional multi-vitamin proteingrass and feeding method thereof
CN116831224A (en) * 2023-07-26 2023-10-03 楚雄师范学院 Preparation method of fermented pennisetum sinese feed

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CN101579041A (en) * 2009-06-14 2009-11-18 中国热带农业科学院热带作物品种资源研究所 Preparation method of biological feed of banana stem leaves
CN102599349A (en) * 2012-04-09 2012-07-25 海南省农垦科学院 Ganoderma waste culture medium feed with grain-saving effect and safe cultivation method by use of the same
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CN102599349A (en) * 2012-04-09 2012-07-25 海南省农垦科学院 Ganoderma waste culture medium feed with grain-saving effect and safe cultivation method by use of the same
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* Cited by examiner, † Cited by third party
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CN109043140A (en) * 2018-09-06 2018-12-21 南充市嘉陵区黄家楼优土生态种养专业合作社 A kind of lactic acid bacteria Jujun grasses formula forage being effectively improved herbivore mouthfeel
CN110089619A (en) * 2019-06-03 2019-08-06 潘国雄 A kind of Chinese medicine bacteria preparation fermented feed and preparation method thereof
CN110506835A (en) * 2019-09-24 2019-11-29 覃小柳 The method for preparing animal feed using microbial fermentation Jujun grasses
CN110537624A (en) * 2019-09-24 2019-12-06 谢新佑 preparation method of Jujun grass fermented feed
CN111387341A (en) * 2020-03-30 2020-07-10 广西大学 Method for processing wangcao and bacterium enzyme synergistic fermentation composition thereof
CN111772038A (en) * 2020-07-31 2020-10-16 宝绿康(北京)生物科技研究院 Antibiotic-free feed containing juncao and protein mulberry or broussonetia papyrifera, preparation method and application
CN113424902A (en) * 2021-04-30 2021-09-24 周川 Beef cattle feed capable of improving immunity and preparation method thereof
CN114052127A (en) * 2021-11-10 2022-02-18 贵州省草业研究所 Complete feed for improving growth rate of black goat lambs and preparation method thereof
CN114365794A (en) * 2022-01-18 2022-04-19 北京平安幸福农业发展有限公司 Functional multi-vitamin proteingrass and feeding method thereof
CN116831224A (en) * 2023-07-26 2023-10-03 楚雄师范学院 Preparation method of fermented pennisetum sinese feed
CN116831224B (en) * 2023-07-26 2024-01-26 楚雄师范学院 Preparation method of fermented pennisetum sinese feed

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Application publication date: 20170623