CN110537624A

CN110537624A - preparation method of Jujun grass fermented feed

Info

Publication number: CN110537624A
Application number: CN201910902067.7A
Authority: CN
Inventors: 谢新佑
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-09-24
Filing date: 2019-09-24
Publication date: 2019-12-06

Abstract

the invention relates to a preparation method of a Jujun grass fermented feed, and belongs to the technical field of breeding feeds. According to the invention, a large amount of cellulose and lignin contained in the pennisetum hydridum are subjected to biochemical action, so that macromolecular carbohydrates which cannot be digested by livestock and poultry are converted into low-molecular carbohydrates which can be digested and absorbed. The cellulose and lignin in the Jujun grass are reduced by times, and the protein is increased by times. The beneficial bacteria added in the preparation process can absorb a large amount of organic nitrogen and inorganic nitrogen which are difficult to utilize by livestock and poultry, and convert the organic nitrogen and the inorganic nitrogen into usable mycoprotein, so that the pennisetum hydridum fermented feed prepared by the method can be used as a feed source for livestock and poultry, and the meat quality and taste are greatly improved. The method has the advantages of simplified process, convenience for mechanized large-scale production and lower cost, can purify water quality, increase dissolved oxygen, has no secondary pollution and improves water environment when being applied to aquaculture.

Description

Preparation method of Jujun grass fermented feed

Technical Field

the invention relates to a preparation method of a Jujun grass fermented feed, and belongs to the technical field of breeding feeds.

Background

Jujun grass is a novel grass seed which is researched and cultivated by professor introduced from Africa for more than 30 years in agriculture and forestry university Rizhao 29114in Fujian province, is popularized to 103 countries in the world, is planted in many provinces and cities in China, is widely applied and has mature technology. Pennisetum alopecuroides of Jujun grass family belongs to four-carbon plants, has high photosynthesis rate which is twice that of wheat, developed root system, high tillering speed, enlarged leaf area, strong stress resistance, adaptability to various soils, seldom occurrence of plant diseases and insect pests in the whole growth period, high growth speed, the average plant height of planted plants in the same year up to 4-5 meters, more than 20 tons of fresh grass produced per mu per year, and more than 6 tons of carbon dioxide absorbed per mu in the south. The stalks are rich in nutrition, contain 16.92 percent of crude protein, 3 to 4 times of the corn stalks, 1.74 percent of crude fat and 8.3 percent of total sugar, the Jujun grass contains rich lignocellulose and increases along with the extension of the growth period: 26.79% in 1.5 months, 47.70% in 6 months, 53.87% in 9 months and 57.90% in 10 months. The microorganisms capable of producing cellulase can hydrolyze lignocellulose into fermentable sugar, so the Jujun grass is a good feed source.

however, the pennisetum hydridum has great limitation as a feed for directly feeding livestock and poultry. Since it contains a large amount of crude fiber and lignin, palatability and digestibility are greatly affected, and necessary processing and fermentation must be performed. In this regard, several researchers have proposed a strategy for fermenting feeds with pennisetum sinese. For example, application No. 201710020509 "A method for preparing fodder for cattle, sheep and herbivores by fermenting Jujun grass", the technology ferments Jujun grass to different degrees, so that the Jujun grass as fodder has improved palatability and nutritive value to different degrees, and some diseases resistance to animals is also improved, but the following disadvantages exist:

(1) the fermentation of the feed is not sufficient. Jujun grass is not fully refined and softened, lignocellulose is not fully converted, and the Jujun grass can also adapt to cattle, sheep and herbivores, but can not adapt to livestock, poultry and aquatic livestock, and can not be fully digested and absorbed.

(2) The function of the fermentation flora is not comprehensive, the effect of inhibiting harmful mixed bacteria is not achieved, and after the feed is digested by animal intestinal tracts, excrement has strong odor, so that the feed is unfavorable for environmental protection and water pollution prevention.

(3) The preparation process of the feed is complex, for example, the secondary fermentation process comprises the steps of mixing and sealing the zymophyte with a culture medium consisting of the giant fungus grass and more than ten materials for the first time, fermenting for 8-10 days at 35-40 ℃, removing the seal for the second time, adding the mixed enzyme, the papaya peel and the like, and fermenting for 2-3 days at 40-45 ℃ after sealing, so that the cost of the feed is higher.

Disclosure of Invention

the invention aims to provide a preparation method of a Jujun grass fermented feed, which improves the prior art and prepares the Jujun grass fermented feed with full fermentation, high nutritional value, strong disease resistance, good meat quality grade and good influence on the environment.

the preparation method of the pennisetum sinese roxb fermented feed provided by the invention comprises the following steps:

(1) Cutting the harvested fresh Jujun grass into pieces with the length of 0.1-3 cm, kneading, and micronizing;

(2) the preparation method of the fermentation inoculant aqueous solution comprises the following specific steps:

(2-1) mixing lactic acid bacteria, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria to obtain zymocyte, wherein the mixing weight percentage is as follows:

(2-2) mixing the zymophyte obtained in the step (2-1), brown sugar and water to obtain a mixture, wherein the weight ratio of the mixture is as follows: fermenting bacteria, namely brown sugar and water in a ratio of 1:1:8, dissolving the mixture at 35 ℃, uniformly stirring, sealing, placing at 25-35 ℃, fermenting for 2-4 days to give out acid smell to obtain a fermenting bacteria activating solution;

(2-3) mixing 9-12 parts of zymophyte activating solution obtained in the step (2-2), 10-13 parts of urea, 4-6 parts of ammonium sulfate, 2.5-5 parts of brown sugar, 2-3 parts of salt, 4-7 parts of trace element soluble compound and 70-85 parts of water, dissolving and uniformly stirring to obtain a zymophyte agent aqueous solution; the trace element soluble compound is formed by mixing manganese sulfate, zinc sulfate monohydrate, ferrous sulfate and calcium hydrophosphate, and the mixing weight percentage is as follows: 28% of manganese sulfate, 22% of zinc sulfate monohydrate, 24% of ferrous sulfate and 26% of calcium hydrophosphate;

(3) Mixing the aqueous solution of the fermentation inoculum obtained in the step (2-3) with the pennisetum sinese roxb obtained in the step (1) according to the weight ratio: mixing the aqueous solution of the fermentation inoculum with herba Jujuni (0.034-0.037) at a ratio of 1, stirring, and kneading to obtain mixture;

(4) and (4) bagging the mixture obtained in the step (3), winding and tightly binding the mixture by using a plastic film to form a sealed airtight bag, fermenting the airtight bag at the temperature of 15-35 ℃ for 7-25 days, and smelling acid smell to obtain the pennisetum sinese roxb fermented feed.

the preparation method of the pennisetum sinese roxb fermented feed provided by the invention has the following advantages:

1. according to the invention, the pennisetum hydridum fermented feed prepared by the method is characterized in that a large amount of cellulose and lignin contained in pennisetum hydridum are subjected to biochemical action, and macromolecular carbohydrates which cannot be digested by livestock and poultry are converted into low molecular carbohydrates which can be digested and absorbed. The cellulose and lignin in the Jujun grass are reduced by times, and the protein is increased by times. The beneficial bacteria added in the preparation process can absorb a large amount of organic nitrogen and inorganic nitrogen which are difficult to utilize by livestock and poultry, and convert the organic nitrogen and the inorganic nitrogen into bacterial protein which can be utilized, thereby expanding the feed sources of the livestock and the poultry. Therefore, the pennisetum hydridum fermented feed prepared by the method can be used as a feed source for livestock and poultry, and the aqueous solution of various zymophytes is fully mixed with pennisetum hydridum subjected to micronization treatment, and the mixture is highly permeated and fermented, so that coarse and hard crude fibers and lignin are changed into soft, juicy and delicious feed, and the nutritional value is doubled.

2. the meat quality and taste of the livestock and poultry fed by the pennisetum hydridum fermented feed prepared by the method are greatly improved. The raw materials of the Jujun grass fermented feed are completely taken from the nature, and no antibiotics or growth hormones are added. The invention effectively inhibits harmful bacteria and pathogenic microorganisms in the feed through the zymophyte, effectively inhibits the propagation of pathogenic bacteria in intestinal tracts of livestock and poultry, blocks the way that various pathogenic bacteria enter digestive tracts, enhances the immunity, improves the capability of resisting diseases, reduces the occurrence of diseases, simultaneously improves the quality and taste of the meat of the livestock, the poultry and aquatic products, and ensures the food safety.

3. The preparation method of the pennisetum hydridum fermented feed is simple and convenient in manufacturing process and suitable for large-scale and mechanized production. The 7 selected zymophytes form the synergistic effect of the mutual promotion advantages of the floras, and can generate a plurality of beneficial enzymes for synergistic fermentation, so that the process of adding additional enzymes for secondary fermentation is omitted, the process is simplified, the mechanical large-scale mass production is more convenient, and the cost is lower.

4. The preparation method of the pennisetum hydridum fermented feed is sanitary and environment-friendly. The beneficial bacteria in the intestines and stomach of the livestock and poultry improve the environment of the digestive tract, not only degrade complex organic matters and facilitate digestion and absorption, but also suppress the growth and reproduction of harmful bacteria and enhance the immunity. Greatly reduces the odor of the excrement of the livestock and the poultry and purifies the environment. The invention is applied to aquaculture, can purify water quality, increase dissolved oxygen, has no secondary pollution, and improves water environment.

5. The preparation method of the pennisetum hydridum fermented feed creates conditions for low-cost and high-efficiency development of feed industry. The raw materials used are wide in source, low in price and mature in technology and equipment, and considerable economic benefits, environmental benefits and social benefits can be produced if the method can be implemented vigorously.

Detailed Description

the invention cuts off the giant fungus grass to the millimeter level, kneads the giant fungus grass, enables the zymocyte liquid to fully permeate, has large solid-liquid contact area, full biochemical reaction and soft texture, and is more suitable for livestock, poultry and aquatic animals. Prior to the present invention, no document has been provided for preparing fermented feed by cutting the grass into millimeter-sized pieces.

the fermentation strain adopted by the invention has comprehensive compatibility, contains 7 bacteria such as lactobacillus, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria, photosynthetic bacteria and the like, retains the lactobacillus, the saccharomycetes and the bifidobacterium, replaces the bacillus licheniformis with the bacillus subtilis, and increases the actinomycetes, the acetic acid bacteria and the photosynthetic bacteria. The functions are more comprehensive and mutually promoted, so that the nutrient substances are multiplied, the disease prevention and resistance capability is strong, and the odor of excrement is basically eliminated. The combination of the bacillus subtilis and the saccharomycetes increases the fermentation effect of enzyme, and the enzyme and the saccharomycetes are mutually promoted, so that the fermentation effect is greatly enhanced, a secondary fermentation process in the prior art is omitted, the preparation process is simplified, and the production cost is low.

the functions and effects of various bacteria constituting the zymophyte of the invention are as follows:

Lactic acid bacteria in the zymophyte can generate a large amount of lactic acid to play a role in the feed by fermenting carbohydrate, so that crude fiber is degraded into monosaccharide which is easy to digest by animals; on the other hand, the compound feed can degrade anti-nutritional factors in the feed, has an inhibiting effect on certain fungi, adsorbs and degrades aflatoxin, and achieves a detoxification effect. Lactic acid bacteria and bifidobacteria combine to have the effect of phagocytizing harmful bacteria on the one hand and correspond to natural autoimmunity by occupying sites in the intestinal tract on the other hand. The lactobacillus can obviously improve the survival rate of the chicks and prevent the occurrence of diarrhea of the piglets. The lactic acid bacteria chelate with trace elements, so that the trace elements are easier to be absorbed by animals.

The yeast in the zymocyte is added into the feed to promote the reproduction of microorganisms, regulate the balance of microorganisms in animal intestinal tracts, improve the digestibility of the feed, increase the appetite, promote the growth of livestock and poultry, and enhance the immunity and disease resistance. The yeast contains rich enzymes such as pepsin, amylase and the like, generally has a digestibility as high as 80-90%, and can utilize cellulosic raw materials to produce feed with high nutritive value, so that the protein content is greatly improved. The yeast can be used as protein supplement of animal feed, and has effects of promoting animal growth, improving meat quality and lean meat percentage, increasing meat quantity, shortening breeding cycle, and enhancing disease resistance of young livestock and poultry.

the bifidobacterium in the zymocyte is an important beneficial microorganism in the intestinal tract, can inhibit the growth of harmful bacteria in the animal body, resist the infection of pathogenic bacteria, enhance the immunity, synthesize vitamins required in the animal body, promote the absorption of calcium, phosphorus, iron and vitamins, generate lactic acid, acetic acid and the like to stimulate the intestinal tract to creep, promote defecation, inhibit the putrefaction of substances in the intestinal tract, purify the intestinal tract environment, decompose carcinogenic substances, stimulate the immune system to improve the disease resistance, and simultaneously can eliminate the foul smell of excrement by matching with light, bacteria and bacillus subtilis, thereby being beneficial to improving the environment.

The bacillus subtilis in the zymocyte replaces the bacillus licheniformis in the prior art, and is beneficial to livestock, poultry and aquatic animals for the following reasons:

1. the bacillus subtilis can generate various digestive enzymes, helps animals to digest and absorb nutrient substances, and can secrete various endogenous enzymes such as protease, amylase, lipase and cellulase to jointly act, so that the digestibility of the feed is improved. The secreted pectinase, glucanase, cellulase and the like have strong functions of decomposing cellulose, lignin and the like in the feed.

2. Can produce abundant beneficial metabolites, synthesize various organic acids, enzymes, bioactivators and other various nutrients which are easy to be utilized. Can synthesize various B vitamins such as vitamin B1, B2, B6, nicotinic acid and the like, and improve the activity of interferon in animals.

3. Has strong antibacterial and pest killing effects, can play a role of antibiotics, has the advantage of occupying space, can inhibit the growth and reproduction of harmful microorganisms such as harmful bacteria, pathogenic bacteria and the like, and can improve the immunity of the colony.

4. strong deodorization capability, can decompose organic substances generating odorous gases, such as organic sulfides, organic nitrogen and the like, and greatly improve the environment of places.

5. can enhance the decomposition capability of organic matters, release high-activity decomposition enzyme during fermentation and multiplication, and decompose difficultly decomposed macromolecular substances into utilizable micromolecular substances.

the invention adds actinomycetes in the zymocyte, which can further improve the disease prevention and resistance of animals, and many secondary metabolites with complex structures generated by the actinomycetes have the functions of medicine and plant protection. Has antibacterial, antifungal and antitumor effects. About 70% of the currently found biologically active substances of several tens of thousands of microbial origin are various secondary metabolites synthesized by actinomycetes, such as antibiotics, organic acids, amino acids, vitamins, steroids, enzymes and enzyme inhibitors, immunomodulators and the like.

the acetic acid bacteria in the zymocyte can kill harmful bacteria more effectively, increase beneficial bacterial colony, clear intestinal tract and help digestion. The acetic acid bacteria can directly convert glucose into acetic acid when the sugar source is sufficient, and convert into acetaldehyde to regenerate acetic acid when the sugar source is insufficient. The invention adds enough brown sugar to ensure that the sugar source is sufficient, has anaerobic condition and is beneficial to being converted into acetic acid.

the photosynthetic bacteria in the zymophyte are added to be used in the feed, and the following functions and effects can be improved:

1. Photosynthetic bacteria are biological agents for purifying the environment with the greatest development prospect in the world at present, and can synthesize organic waste into self mycoprotein and other harmless substances by using light energy. It can be used as feed for livestock, poultry and aquatic products for enhancing immunity, reducing digestive tract diseases, purifying intestinal tract, and eliminating feces odor.

2. the photosynthetic bacteria are rich in protein, vitamins, coenzyme Q and pigment, can obviously improve the survival rate and disease resistance of animals when being added into feed, reduce the occurrence of diseases, can quickly increase the weight and has good meat quality.

3. the photosynthetic bacteria can purify water quality, increase dissolved oxygen, improve culture density, promote growth, shorten culture period, enhance immunity, and prevent and treat diseases when used in aquaculture. The fish pond does not use or use less antibiotic medicines, and the meat quality and taste are improved.

The brown sugar in the zymophyte is added in two stages, firstly, the brown sugar is added in the zymophyte activating solution as a nutrient substance to promote vigorous growth and vigorous propagation of each strain, so that the number of the zymophyte is more and the quality is better. Secondly, brown sugar is added into the aqueous solution of the leaven, so that the sugar source of the Jujun grass is more sufficient, the fermentation is more sufficient, and the effect is better.

the following describes embodiments of the method of the invention:

example 1

(1) Cutting the harvested fresh Jujun grass into pieces with the length of 1mm, rubbing, and performing micronization treatment;

(2-1) mixing lactic acid bacteria, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria to obtain zymocyte, wherein the mixing weight percentage is as follows: 27.5% of lactic acid bacteria, 20% of saccharomycetes, 18% of bacillus subtilis, 12.5% of actinomycetes, 2% of bifidobacterium, 13% of acetic acid bacteria and 7% of photosynthetic bacteria;

(2-2) mixing the zymophyte obtained in the step (2-1), brown sugar and water to obtain a mixture, wherein the mixing weight ratio is as follows: fermenting bacteria, namely brown sugar and water in a ratio of 1:1:8, dissolving the mixture at 35 ℃, stirring uniformly, sealing, placing at 35 ℃, fermenting for 2 days to give out acid smell to obtain a fermenting bacteria activating solution;

(2-3) mixing 12 parts of zymophyte activating solution obtained in the step (2-2), 10 parts of urea, 5 parts of ammonium sulfate, 2.5 parts of brown sugar, 2.5 parts of salt, 7 parts of trace element soluble compound and 70 parts of water, completely dissolving, and uniformly stirring to obtain a zymophyte agent aqueous solution; the trace element soluble compound is formed by mixing manganese sulfate, zinc sulfate monohydrate, ferrous sulfate and calcium hydrophosphate, and the mixing weight percentage is as follows: 28% of manganese sulfate, 22% of zinc sulfate monohydrate, 24% of ferrous sulfate and 26% of calcium hydrophosphate;

(3) Mixing the aqueous solution of the fermentation inoculum obtained in the step (2-3) with the pennisetum sinese roxb obtained in the step (1) according to the weight ratio: mixing the aqueous solution of the fermentation inoculum with herba Jujuni (0.034: 1), stirring, and kneading to obtain mixture;

(4) and (4) bagging the mixture obtained in the step (3), winding and tightly binding the mixture by using a plastic film to form a sealed airtight bag, fermenting the airtight bag at the temperature of 35 ℃ for 7 days, and smelling acid smell to obtain the pennisetum sinese roxb fermented feed.

Example 2

(1) Cutting the harvested fresh Jujun grass into pieces with a length of 3mm, kneading, and micronizing;

(2-1) mixing lactic acid bacteria, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria to obtain zymocyte, wherein the mixing weight percentage is as follows: 25% of lactic acid bacteria, 24% of saccharomycetes, 24% of bacillus subtilis, 10% of actinomycetes, 3% of bifidobacteria, 4% of acetic acid bacteria and 10% of photosynthetic bacteria;

(2-2) mixing the zymophyte obtained in the step (2-1), brown sugar and water to obtain a mixture, wherein the mixing weight ratio is as follows: fermenting bacteria, namely brown sugar and water in a ratio of 1:1:8, dissolving the mixture at 35 ℃, uniformly stirring, sealing, placing at 25 ℃, and fermenting for 4 days to give out acid smell to obtain a fermenting bacteria activating solution;

(2-3) mixing 11 parts of zymophyte activating solution obtained in the step (2-2), 10.5 parts of urea, 4.5 parts of ammonium sulfate, 4 parts of brown sugar, 2.5 parts of salt, 6 parts of trace element soluble compound and 75 parts of water, completely dissolving, and uniformly stirring to obtain a zymophyte agent aqueous solution; the trace element soluble compound is formed by mixing manganese sulfate, zinc sulfate monohydrate, ferrous sulfate and calcium hydrophosphate, and the mixing weight percentage is as follows: 28% of manganese sulfate, 22% of zinc sulfate monohydrate, 24% of ferrous sulfate and 26% of calcium hydrophosphate;

(3) Mixing the aqueous solution of the fermentation inoculum obtained in the step (2-3) with the pennisetum sinese roxb obtained in the step (1) according to the weight ratio: and (3) fermenting agent aqueous solution: mixing herba Jujun (Jujun grass) 0.037: 1, stirring, and kneading to obtain mixture;

(4) and (4) bagging the mixture obtained in the step (3), winding and tightly binding the mixture by using a plastic film to form a sealed airtight bag, fermenting the bag at the temperature of 28 ℃ for 12 days, and smelling acid smell to obtain the pennisetum sinese roxb fermented feed.

Example 3

(1) Cutting the harvested fresh Jujun grass into pieces with the length of 15mm, rubbing, and performing micronization treatment;

(2-1) mixing lactic acid bacteria, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria to obtain zymocyte, wherein the mixing weight percentage is as follows: 30% of lactic acid bacteria, 18% of saccharomycetes, 19% of bacillus subtilis, 16% of actinomycetes, 5% of bifidobacteria, 3% of acetic acid bacteria and 9% of photosynthetic bacteria;

(2-2) mixing the zymophyte obtained in the step (2-1), brown sugar and water to obtain a mixture, wherein the mixing weight ratio is as follows: fermenting bacteria, namely brown sugar and water in a ratio of 1:1:8, dissolving the mixture at 35 ℃, uniformly stirring, sealing, placing at 30 ℃, and fermenting for 3 days to give out acid smell to obtain a fermenting bacteria activating solution;

(2-3) mixing 10 parts of zymophyte activating solution obtained in the step (2-2), 11 parts of urea, 4 parts of ammonium sulfate, 5 parts of brown sugar, 3 parts of salt, 5 parts of trace element soluble compound and 85 parts of water, completely dissolving, and uniformly stirring to obtain a zymophyte agent aqueous solution; the trace element soluble compound is formed by mixing manganese sulfate, zinc sulfate monohydrate, ferrous sulfate and calcium hydrophosphate, and the mixing weight percentage is as follows: 28% of manganese sulfate, 22% of zinc sulfate monohydrate, 24% of ferrous sulfate and 26% of calcium hydrophosphate;

(3) mixing the aqueous solution of the fermentation inoculum obtained in the step (2-3) with the pennisetum sinese roxb obtained in the step (1) according to the weight ratio: and (3) fermenting agent aqueous solution: mixing herba Jujun (Jujun grass) 0.036: 1, stirring, and kneading to obtain mixture;

(4) And (4) bagging the mixture obtained in the step (3), winding and tightly binding the mixture by using a plastic film to form a sealed airtight bag, fermenting the airtight bag at the temperature of 24 ℃ for 18 days, and smelling acid smell to obtain the pennisetum sinese roxb fermented feed.

Example 4

(1) Cutting the harvested fresh Jujun grass into pieces with the length of 3cm, kneading, and micronizing;

(2-1)) mixing lactic acid bacteria, saccharomycetes, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria to obtain zymocyte, wherein the mixing weight percentage is as follows: 27% of lactic acid bacteria, 21% of saccharomycetes, 20% of bacillus subtilis, 13.5% of actinomycetes, 3.5% of bifidobacterium, 6.5% of acetic acid bacteria and 8.5% of photosynthetic bacteria;

(2-2) mixing the zymophyte obtained in the step (2-1), brown sugar and water to obtain a mixture, wherein the mixing weight ratio is as follows: fermenting bacteria, namely brown sugar and water in a ratio of 1:1:8, dissolving the mixture at 35 ℃, uniformly stirring, sealing, placing at 28 ℃, and fermenting for 3 days to give out acid smell to obtain a fermenting bacteria activating solution;

(2-3) mixing 9 parts of zymophyte activating solution obtained in the step (2-2), 13 parts of urea, 6 parts of ammonium sulfate, 3.5 parts of brown sugar, 2 parts of salt, 4 parts of trace element soluble compound and 80 parts of water, completely dissolving, and uniformly stirring to obtain a zymophyte agent aqueous solution; the trace element soluble compound is formed by mixing manganese sulfate, zinc sulfate monohydrate, ferrous sulfate and calcium hydrophosphate, and the mixing weight percentage is as follows: 28% of manganese sulfate, 22% of zinc sulfate monohydrate, 24% of ferrous sulfate and 26% of calcium hydrophosphate;

(3) Mixing the aqueous solution of the fermentation inoculum obtained in the step (2-3) with the pennisetum sinese roxb obtained in the step (1) according to the weight ratio: mixing the aqueous solution of the fermentation inoculum with Jujun grass (0.035: 1), stirring, and kneading to obtain mixture;

(4) and (4) bagging the mixture obtained in the step (3), winding and tightly binding the mixture by using a plastic film to form a sealed airtight bag, fermenting the bag at the temperature of 15 ℃ for 25 days, and smelling acid smell to obtain the pennisetum sinese roxb fermented feed.

In the above embodiments of the present invention, the lactic acid bacteria, yeast, bacillus subtilis, actinomycetes, bifidobacterium, acetic acid bacteria and photosynthetic bacteria used in the method can be purchased from food and chemical industry shops, and the effective viable counts are respectively: 45-55 hundred million/g lactic acid bacteria, 35-40 hundred million/g yeast, 25-35 hundred million/g bacillus subtilis, 25-30 hundred million/g actinomycetes, 5-10 hundred million/g bifidobacterium, 20-25 hundred million/g acetic acid bacteria and 15-20 hundred million/g photosynthetic bacteria.

Claims

1. A preparation method of a Jujun grass fermented feed is characterized by comprising the following steps: