CN105237147A - Culture medium for oyster mushroom and preparation method thereof - Google Patents

Culture medium for oyster mushroom and preparation method thereof Download PDF

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Publication number
CN105237147A
CN105237147A CN201510554441.0A CN201510554441A CN105237147A CN 105237147 A CN105237147 A CN 105237147A CN 201510554441 A CN201510554441 A CN 201510554441A CN 105237147 A CN105237147 A CN 105237147A
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fermentation
culture medium
raw material
preparation
oyster mushroom
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韦波
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Guangxi Nanning Shengqian Technology Development Co Ltd
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Guangxi Nanning Shengqian Technology Development Co Ltd
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Abstract

The invention discloses a culture medium for oyster mushrooms and a preparation method thereof and belongs to the technical field of edible mushroom culture mediums. The culture medium is prepared from following components, in a mixing manner, wood chips from broad-leaved trees, bran, rice bran, turf, corn flour, lime powder, sugar, calcium superphosphate, potassium sulfate, magnesium sulfate and an organic fermentation material. The organic fermentation material is prepared with excrement from poultry and livestock, silkworm excrement, biogas residue and crop straw as raw materials with addition of a fermentation inoculant through stacking and natural fermentation. In the invention, a high-effective composite bacteria strain, which is composed of various bacteria as components in special proportion, is employed to ferment waste materials, so that fermentation time is reduced, fermentation efficiency and the capability of decomposing animal excrements into harmless substances are improved, thereby eliminating stink generated during fermentation. The culture medium also can increase the content of organic substances after fermentation of the crop straw and improve the nutrients in fertilizer produced through fermentation. The culture medium also can form a strong probiotic environment and improve anti-disease capability of edible mushrooms.

Description

A kind of Oyster mushroom culture medium and preparation method thereof
Technical field
The invention belongs to edible fungus culturing matrix technology field, be specifically related to a kind of Oyster mushroom culture medium and preparation method thereof.
Background technology
Edible mushrooms refers to that sporophore is very large, gill fungus bacterium (macro fungi) that can be edible, is commonly referred to as mushroom.The known edible mushrooms of China has kind more than 350, wherein belongs to Basidiomycotina more, common are: mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake (matsutake), dried mushroom, russule, glossy ganoderma, Chinese caterpillar fungus, truffle, lark and bolete etc.; Minority belongs to Ascomycotina, Qi Zhongyou: morel, saddle fungus, ferfas etc.
Edible mushrooms is not only delicious, and nutritious, is often called heath food by people.The protein content of mushroom class is generally fresh mushroom 1.5-6%, dry mushroom 15-35%, and higher than general vegetables, and its amino acid ratio of components is comparatively comprehensive, and most mushroom class contains eight seed amino acids of needed by human, and wherein in mushroom, straw mushroom, needle mushroom, lysine content enriches.And mushroom class contains multivitamin and the multiple mineral element with physiologically active, as VB1, VB12, Vc, Vk, VD and phosphorus, sodium, potassium, calcium, iron and many trace elements, the deficiency in other food can be supplemented.Edible mushrooms also has good pharmaceutical use simultaneously.In edible mushrooms containing biologically active substance as: macromolecule polysaccharide, β-glucose and RNA complex body, natural organic germanium, nucleic acid degradation product, cAMP and triterpene compound etc. to safeguarding that HUMAN HEALTH has important utility value, now often by businessman with its for raw material production prepare protective foods, nourishing drink, wine and medicine in a large number for medicinal and input health-product market.
The mode of edible fungus culturing is varied.Divide by culture material, have segment wood cultivated and substituting stuff cultivation; Divide by planting type, have ridge cultivation, bed to plant and case cultivation; Divide by cultivation place, have indoor cultivation, outdoor planting etc.Segment wood cultivated fewer and feweri, reason is because destroy forest tree resource.The substituting stuff cultivation of edible mushrooms comprises cultivation in raw material, grog cultivation and fermentation material cultivation.Cultivation in raw material refers to according to the method being directly used in edible fungus culturing after culture material poach of filling a prescription; Grog cultivation refers to according to the method inoculating culturing edible fungus after culture material poach, pack, sterilizing of filling a prescription; Fermentation material cultivation refer to by after culture material poach through fermentation with kill harmful miscellaneous bacteria and worm's ovum, and culture material is become thoroughly decomposed, then for the method for edible fungus culturing.The material such as cotton seed hulls for cultivation in raw material must be fresh, and do not lump, do not go mouldy, temperature is below 15 DEG C.Material for grog and fermentation material cultivation need not be fresh, high temperature, low temperature season.Flat mushroom can use cultivation in raw material; The available fermentation material cultivation such as Twospore Mushroom, Coprinus comatus, Brazilian mushroom, Pleurotus Citrinopileatus Sing; Glossy ganoderma, mushroom, needle mushroom etc. are cultivated with grog.The Conver-table such as Twospore Mushroom, Brazilian mushroom is planted, and the multiplex bag of other edible mushroomss is planted.
Cultivation matrix is the place of the various nutritive substances that edible mushrooms depends on for existence.When using kind identical with growing environment condition, use different, difference of filling a prescription, matrix the processing mode of matrix different, the difference all to some extent such as output, quality, local flavor of edible mushrooms.
Summary of the invention
The object of the present invention is to provide a kind of Oyster mushroom culture medium and preparation method thereof.
Concrete, described Oyster mushroom culture medium is obtained by the component mixed preparing comprising following parts by weight: hardwood sawdust 8-15 part, wheat bran 3-8 part, rice bran 3-8 part, peat composed of rotten mosses 1-3 part, Semen Maydis powder 1-3 part, lime powder 0.1-0.5 part, white sugar 0.1-0.5 part, calcium superphosphate 0.03-0.08 part, potassium sulfate 0.01-0.05 part, magnesium sulfate 0.01-0.05 part, organic fermentation material 3-8 part; The cultivation matrix pH value be mixed with is 5.5-7.5, and water content is at 60-70%; Described organic fermentation material is with feces of livestock and poultry, silkworm excrement, natural pond slag, agricultural crop straw for fermentation raw material, then adds the fermenting agent of fermentation raw material gross weight 5-15%, stacking, spontaneous fermentation 15-25 days and obtain; Described fermenting agent is by following fermented bacterium: Candida utilis (candidautilis), subtilis (Bacillassubtilis), deep shape genus bacillus (Bacillusmycoides), lactic acid streptococcus faecium (Streptococcusfaecalis), lactobacterium casei (Lactobacilluscasei), aspergillus niger (Aspergillusniger), Humicola insolens (Humicolainsolens), through enlarged culturing, the combination liquid bacterial agent be mixed.
As further illustrating of above-mentioned Oyster mushroom culture medium, in described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10-20%, subtilis 10-20%, deep shape genus bacillus 10-20%, lactic acid streptococcus faecium 10-20%, lactobacterium casei 10-20%, aspergillus niger 10-20%, Humicola insolens 10-20%.
As further illustrating of above-mentioned Oyster mushroom culture medium, each raw material weight number ratio of described fermentation raw material is: feces of livestock and poultry 2-4 part, silkworm excrement 0.5-1.5 part, natural pond slag 0.5-1.5 part, agricultural crop straw 1-3 part.
As further illustrating of above-mentioned Oyster mushroom culture medium, described agricultural crop straw is comprise the one or more than one mixture in maize straw, rice straw, wheat stalk, cotton seed hulls, sugarcane leaf, the sugarcane tip, and length is 1-3 centimetre.
Flat mushroom, have another name called pick up the ears, oyster cap fungus, oyster mushroom, black tree peony mushroom, Taiwan, also known as Pleurotus geesteranus, is Agaricales Pleurotaceae one kind under Basidiomycota, is a kind quite common grey edible mushroom.Flat mushroom belongs to wooden saprophytic mushroom, Mierocrystalline cellulose, hemicellulose, xylogen and the ingredient breakdown such as starch, pectin, by the multiple enzyme of secretion, can be become the nutrition such as monose or disaccharide, be absorbed and used as carbon source by mycelia, also directly organic acid and alcohols etc. can be absorbed, but directly inorganic carbon can not be utilized.Flat mushroom is using inorganic nitrogen (ammonium salt, nitrate etc.) and organic nitrogen compound (urea, amino acid, protein etc.) as nitrogenous source.Protein will pass through proteases for decomposing, just can be absorbed and used after becoming amino (different sexes) acid.Mushroom growth also needs certain inorganic salts in growing, wherein important with phosphorus, potassium, magnesium, calcium constituent.The nutrilite wide range of suitable flat mushroom.Deciduous tree Duan Mu is commonly used and wood sawdust makes planting material during artificial culture, China uses again straw (large and small wheat straw), corn cob, Pericarppium arachidis hypogaeae, cotton seed hulls etc. are as culture material, suitable supply nitrogen and other elements such as a little cake fertilizer, calcium superphosphate, lime of arranging in pairs or groups, can impel the good development growth of flat mushroom again.
Deciduous tree refers generally to the trees of dicotyledoneae, and have flat, broader blade, vein reticulates, and leaf is evergreen or fall leaves, and general blade face is broad, the leaf perennial woody plant having various shape with seeds difference.What have is evergreen, and fallen leaves class comes off mostly on autumn and winter season Ye Congzhi.The forest be made up of deciduous tree, is called broad-leaf forest.The economic worth of deciduous tree is large, and be much important commerical tree species, wherein some is famous and precious timber, as camphor tree, nanmu etc.Various fruit is all deciduous tree.Deciduous tree is of a great variety, is referred to as hardwood.Hardwood sawdust is wooden softer comparatively speaking, contributes to decomposition and the absorption of mycelia.
Silkworm excrement is the solid manure of silkworm worm discharge in sericulture process, containing the nutritive substances such as abundant sugar, protein and mineral element and a small amount of plant growth triumph conditioning agent.If silkworm excrement is directly emitted without process can cause the pollution of environment, according to biofermentation technique, biological organic fertilizer can be prepared by crop fermentation raw material, grow fat as treasured.
The peat composed of rotten mosses has another name called " peat ", is the product in formation process of bog, by the residual body of marsh plant, under the anaerobic condition of many water, can not decompose accumulation completely and form.Containing large quantity of moisture with not by the plant residue, soil ulmin and a part of mineral substance that thoroughly decompose, it is the organic substance of pure natural, also be a kind of green matter of aseptic, nontoxic, nuisanceless, pollution-free, noresidue, there is the effect of improvement soil, be widely used in composting industry.
In the fermentation of feces of livestock and poultry, silkworm excrement, natural pond slag, agricultural crop straw the like waste material recycling, fermented bacterium is most important, decides the efficiency of fermentation and the quality of leavened prod.The present invention is through research extremely hard and bitter in a large number, develop with Candida utilis, subtilis, deep shape genus bacillus, lactic acid streptococcus faecium, lactobacterium casei, aspergillus niger, the multiple bacterial classification such as Humicola insolens is the specific proportioning high efficiency composition bacterial classification of component, use it in the fermentation of waste material recycling, fermentation time can be shortened, improve fermentation efficiency and animal excrement are resolved into the ability of innoxious substance, eliminate the stink produced in fermenting process, the organic content after fermenting crops straws can be improved again, the nutrient of the fertilizer produced after improving fermentation, powerful probiotic bacterium environment can also be formed, improve the resistance against diseases of edible mushrooms.
The invention also discloses the preparation method of above-mentioned Oyster mushroom culture medium, concrete, comprise the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: feces of livestock and poultry 2-4 part, silkworm excrement 0.5-1.5 part, natural pond slag 0.5-1.5 part, agricultural crop straw 1-3 part, is as short as 1-3 centimetre by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10-20%, subtilis 10-20%, deep shape genus bacillus 10-20%, lactic acid streptococcus faecium 10-20%, lactobacterium casei 10-20%, aspergillus niger 10-20%, Humicola insolens 10-20%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 5-15%, stirs, stacking, spontaneous fermentation 15-25 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 8-15 part, wheat bran 3-8 part, rice bran 3-8 part, peat composed of rotten mosses 1-3 part, Semen Maydis powder 1-3 part, lime powder 0.1-0.5 part, white sugar 0.1-0.5 part, calcium superphosphate 0.03-0.08 part, potassium sulfate 0.01-0.05 part, magnesium sulfate 0.01-0.05 part, organic fermentation material 3-8 part, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
As the further illustrating of preparation method of above-mentioned Oyster mushroom culture medium, the routine techniques means that described fluid enlargement culture is familiar with by those skilled in the art, are not described in detail in this; In the preparation process of organic fermentation material, for enabling fermentation raw material fully ferment, every 5-7 days, turning process being carried out to fermentation raw material, several times the sprinkling of combining liquid bacterial agent being carried out to fermentation raw material simultaneously.
Compared with prior art, the outstanding beneficial effect that the present invention has comprise following some:
(1) the present invention adopts multiple bacterial classification to be in the fermentation for waste material recycling of the specific proportioning high efficiency composition bacterial classification of component, fermentation time can be shortened, improve fermentation efficiency and animal excrement are resolved into the ability of innoxious substance, eliminate the stink produced in fermenting process, the organic content after fermenting crops straws can be improved again, the nutrient of the fertilizer produced after improving fermentation, can also form powerful probiotic bacterium environment, improves the resistance against diseases of edible mushrooms.
(2) the present invention is according to the growth of flat mushroom and uptake characters, and in strict accordance with scientific formula, composite and Oyster mushroom culture medium that is that obtain is very suitable for the cultivation of flat mushroom; Cultivating white mushroom growing ability is strong, even, the anti-characteristic of disease of long speed is strong.
(3) preparation method of Oyster mushroom culture medium of the present invention is simple, and production cost is low, is easy to promote.
Embodiment
Following examples are described in further detail the present invention, and the present embodiment is only do clearer explanation to the present invention, instead of limitation of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all drop within protection scope of the present invention.
Embodiment 1
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: cow dung 3 parts, silkworm excrement 1 part, natural pond slag 1 part, agricultural crop straw 2 parts, is as short as 2 centimetres by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 15%, subtilis 15%, deep shape genus bacillus 15%, lactic acid streptococcus faecium 15%, lactobacterium casei 15%, aspergillus niger 15%, Humicola insolens 10%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 10%, stirs, stacking, spontaneous fermentation 20 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 10 parts, 5 parts, wheat bran, 8 parts, rice bran, the peat composed of rotten mosses 3 parts, Semen Maydis powder 2 parts, lime powder 0.3 part, white sugar 0.3 part, calcium superphosphate 0.05 part, potassium sulfate 0.03 part, 0.03 part, magnesium sulfate, organic fermentation material 5 parts, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
Embodiment 2
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: chicken manure 2 parts, silkworm excrement 1.5 parts, natural pond slag 1.2 parts, agricultural crop straw 3 parts, is as short as 3 centimetres by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 20%, subtilis 20%, deep shape genus bacillus 10%, lactic acid streptococcus faecium 10%, lactobacterium casei 10%, aspergillus niger 15%, Humicola insolens 15%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 5%, stirs, stacking, spontaneous fermentation 25 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 12 parts, 5 parts, wheat bran, 5 parts, rice bran, the peat composed of rotten mosses 2 parts, Semen Maydis powder 2 parts, lime powder 0.5 part, white sugar 0.5 part, calcium superphosphate 0.08 part, potassium sulfate 0.02 part, 0.02 part, magnesium sulfate, organic fermentation material 3-8 part, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
Embodiment 3
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: pig manure 4 parts, silkworm excrement 0.5 part, natural pond slag 0.5 part, agricultural crop straw 1 part, is as short as 1 centimetre by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10%, subtilis 15%, deep shape genus bacillus 20%, lactic acid streptococcus faecium 15%, lactobacterium casei 15%, aspergillus niger 10%, Humicola insolens 15%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 15%, stirs, stacking, spontaneous fermentation 15 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 8 parts, 88 parts, wheat bran, 3 parts, rice bran, the peat composed of rotten mosses 3 parts, Semen Maydis powder 1 part, lime powder 0.1 part, white sugar 0.2 part, calcium superphosphate 0.04 part, potassium sulfate 0.02 part, 0.05 part, magnesium sulfate, organic fermentation material 8 parts, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
Embodiment 4
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: chicken manure 3 parts, silkworm excrement 1 part, natural pond slag 1 part, agricultural crop straw 2 parts, is as short as 2 centimetres by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 15%, subtilis 10%, deep shape genus bacillus 10%, lactic acid streptococcus faecium 20%, lactobacterium casei 10%, aspergillus niger 20%, Humicola insolens 15%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 12%, stirs, stacking, spontaneous fermentation 22 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 15 parts, 3 parts, wheat bran, 3 parts, rice bran, the peat composed of rotten mosses 3 parts, Semen Maydis powder 3 parts, lime powder 0.4 part, white sugar 0.4 part, calcium superphosphate 0.06 part, potassium sulfate 0.03 part, 0.03 part, magnesium sulfate, organic fermentation material 6 parts, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
Embodiment 5
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: cow dung 4 parts, silkworm excrement 0.5 part, natural pond slag 0.5 part, agricultural crop straw 3 parts, is as short as 1 centimetre by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10%, subtilis 20%, deep shape genus bacillus 10%, lactic acid streptococcus faecium 20%, lactobacterium casei 15%, aspergillus niger 15%, Humicola insolens 10%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 10%, stirs, stacking, spontaneous fermentation 20 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 12 parts, 5 parts, wheat bran, 5 parts, rice bran, the peat composed of rotten mosses 2 parts, Semen Maydis powder 2 parts, lime powder 0.3 part, white sugar 0.3 part, calcium superphosphate 0.05 part, potassium sulfate 0.03 part, 0.02 part, magnesium sulfate, organic fermentation material 6 parts, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
Embodiment 6
The preparation method of Oyster mushroom culture medium of the present invention, comprises the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: cow dung 2 parts, silkworm excrement 1.5 parts, natural pond slag 1.5 parts, agricultural crop straw 2 parts, is as short as 2 centimetres by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 15%, subtilis 15%, deep shape genus bacillus 20%, lactic acid streptococcus faecium 20%, lactobacterium casei 10%, aspergillus niger 10%, Humicola insolens 10%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 12%, stirs, stacking, spontaneous fermentation 20 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 10 parts, 6 parts, wheat bran, 4 parts, rice bran, the peat composed of rotten mosses 1.5 parts, Semen Maydis powder 1.5 parts, lime powder 0.2 part, white sugar 0.3 part, calcium superphosphate 0.05 part, potassium sulfate 0.03 part, 0.03 part, magnesium sulfate, organic fermentation material 8 parts, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.

Claims (5)

1. an Oyster mushroom culture medium, it is characterized in that, obtained by the component mixed preparing comprising following parts by weight: hardwood sawdust 8-15 part, wheat bran 3-8 part, rice bran 3-8 part, peat composed of rotten mosses 1-3 part, Semen Maydis powder 1-3 part, lime powder 0.1-0.5 part, white sugar 0.1-0.5 part, calcium superphosphate 0.03-0.08 part, potassium sulfate 0.01-0.05 part, magnesium sulfate 0.01-0.05 part, organic fermentation material 3-8 part; The cultivation matrix pH value be mixed with is 5.5-7.5, and water content is at 60-70%;
Described organic fermentation material is with feces of livestock and poultry, silkworm excrement, natural pond slag, agricultural crop straw for fermentation raw material, then adds the fermenting agent of fermentation raw material gross weight 5-15%, stacking, spontaneous fermentation 15-25 days and obtain; Described fermenting agent is by following fermented bacterium: Candida utilis, subtilis, deep shape genus bacillus, lactic acid streptococcus faecium, lactobacterium casei, aspergillus niger, Humicola insolens, through enlarged culturing, and the combination liquid bacterial agent be mixed.
2. Oyster mushroom culture medium according to claim 1, it is characterized in that, in described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10-20%, subtilis 10-20%, deep shape genus bacillus 10-20%, lactic acid streptococcus faecium 10-20%, lactobacterium casei 10-20%, aspergillus niger 10-20%, Humicola insolens 10-20%.
3., according to the arbitrary described Oyster mushroom culture medium of claim 1 or 2, it is characterized in that, each raw material weight number ratio of described fermentation raw material is: feces of livestock and poultry 2-4 part, silkworm excrement 0.5-1.5 part, natural pond slag 0.5-1.5 part, agricultural crop straw 1-3 part.
4. Oyster mushroom culture medium according to claim 3, is characterized in that, described agricultural crop straw is comprise the one or more than one mixture in maize straw, rice straw, wheat stalk, cotton seed hulls, sugarcane leaf, the sugarcane tip, and length is 1-3 centimetre.
5., according to the preparation method of the arbitrary described Oyster mushroom culture medium of claim 1-4, it is characterized in that, comprise the following steps:
(1) preparation of fermentation raw material: take following fermentation raw material by following ratio of weight and number: feces of livestock and poultry 2-4 part, silkworm excrement 0.5-1.5 part, natural pond slag 0.5-1.5 part, agricultural crop straw 1-3 part, is as short as 1-3 centimetre by agricultural crop straw hand hay cutter, mixes and get final product;
(2) preparation of liquid bacterial agent is combined: each bacterial classification is carried out fluid enlargement culture separately, You effect Jun Shuo≤1 × 10 alive of gained microbial inoculum 8cFU/g, mixes and get final product; In described combination liquid bacterial agent, the volume percent content of each microbial inoculum is: Candida utilis 10-20%, subtilis 10-20%, deep shape genus bacillus 10-20%, lactic acid streptococcus faecium 10-20%, lactobacterium casei 10-20%, aspergillus niger 10-20%, Humicola insolens 10-20%;
(3) preparation of organic fermentation material: combination liquid bacterial agent is evenly sprayed on fermentation raw material by fermentation raw material gross weight 5-15%, stirs, stacking, spontaneous fermentation 15-25 days and get final product;
(4) preparation of Oyster mushroom culture medium: take following component by following ratio of weight and number: hardwood sawdust 8-15 part, wheat bran 3-8 part, rice bran 3-8 part, peat composed of rotten mosses 1-3 part, Semen Maydis powder 1-3 part, lime powder 0.1-0.5 part, white sugar 0.1-0.5 part, calcium superphosphate 0.03-0.08 part, potassium sulfate 0.01-0.05 part, magnesium sulfate 0.01-0.05 part, organic fermentation material 3-8 part, mix; Controlling cultivation matrix pH value is 5.5-7.5, and water content, at 60-70%, to obtain final product.
CN201510554441.0A 2015-09-01 2015-09-01 Culture medium for oyster mushroom and preparation method thereof Pending CN105237147A (en)

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CN107434715A (en) * 2016-05-26 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving white ginseng bacterium yield
CN107434723A (en) * 2016-05-27 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving rainbow conk yield
CN107434548A (en) * 2016-05-26 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving Yield of Pleurotus Ostreatus
CN108184543A (en) * 2018-01-30 2018-06-22 新平源健农业开发有限公司 A kind of Oyster mushroom culture medium and preparation method thereof
CN108207485A (en) * 2017-12-29 2018-06-29 凤台县鼎足农业发展有限公司 It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method
CN108887083A (en) * 2018-07-26 2018-11-27 合肥仙之峰农业科技有限公司 A kind of implantation methods of oyster mushroom
CN110140592A (en) * 2019-05-22 2019-08-20 遂宁市秭源农业开发有限公司 A kind of oyster mushroom culture medium and preparation method thereof
CN112154859A (en) * 2020-08-26 2021-01-01 河北省科学院生物研究所 Oyster mushroom cultivation material and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104109005A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Flammulina velutipes culture medium prepared from cotton straw and preparation method thereof
CN104109021A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Flammulina velutipe culture medium taking corncobs as raw materials and preparation method thereof
CN104341236A (en) * 2014-07-22 2015-02-11 广州甘蔗糖业研究所 Preparation method and application of pleurotus ostreatus culture medium by using bagasse
CN104355812A (en) * 2014-10-31 2015-02-18 颍上县永祥旱粮研究所 Oyster mushroom strain cultivation medium
CN104478515A (en) * 2014-12-08 2015-04-01 西华大学 Fermentation liquid for culturing pleurotus eryngii matrix, pleurotus eryngii culture medium and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104109005A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Flammulina velutipes culture medium prepared from cotton straw and preparation method thereof
CN104109021A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Flammulina velutipe culture medium taking corncobs as raw materials and preparation method thereof
CN104341236A (en) * 2014-07-22 2015-02-11 广州甘蔗糖业研究所 Preparation method and application of pleurotus ostreatus culture medium by using bagasse
CN104355812A (en) * 2014-10-31 2015-02-18 颍上县永祥旱粮研究所 Oyster mushroom strain cultivation medium
CN104478515A (en) * 2014-12-08 2015-04-01 西华大学 Fermentation liquid for culturing pleurotus eryngii matrix, pleurotus eryngii culture medium and preparation method thereof

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107434715A (en) * 2016-05-26 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving white ginseng bacterium yield
CN107434548A (en) * 2016-05-26 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving Yield of Pleurotus Ostreatus
CN107434723A (en) * 2016-05-27 2017-12-05 临桂铭泰果蔬农民专业合作社 A kind of planting material for improving rainbow conk yield
CN105859452A (en) * 2016-06-21 2016-08-17 广德县富丰蔬菜专业合作社 Organic Pleurotus ostreatus culture medium
CN108207485A (en) * 2017-12-29 2018-06-29 凤台县鼎足农业发展有限公司 It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method
CN108184543A (en) * 2018-01-30 2018-06-22 新平源健农业开发有限公司 A kind of Oyster mushroom culture medium and preparation method thereof
CN108887083A (en) * 2018-07-26 2018-11-27 合肥仙之峰农业科技有限公司 A kind of implantation methods of oyster mushroom
CN110140592A (en) * 2019-05-22 2019-08-20 遂宁市秭源农业开发有限公司 A kind of oyster mushroom culture medium and preparation method thereof
CN112154859A (en) * 2020-08-26 2021-01-01 河北省科学院生物研究所 Oyster mushroom cultivation material and preparation method thereof

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Application publication date: 20160113