CN104341236A - Preparation method and application of pleurotus ostreatus culture medium by using bagasse - Google Patents

Preparation method and application of pleurotus ostreatus culture medium by using bagasse Download PDF

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Publication number
CN104341236A
CN104341236A CN201410351654.9A CN201410351654A CN104341236A CN 104341236 A CN104341236 A CN 104341236A CN 201410351654 A CN201410351654 A CN 201410351654A CN 104341236 A CN104341236 A CN 104341236A
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CN
China
Prior art keywords
bagasse
matrix
mushroom
cultivation
medium
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Pending
Application number
CN201410351654.9A
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Chinese (zh)
Inventor
周文灵
江永
李奇伟
卢颖林
敖俊华
陈仲华
黄莹
陈迪文
黄振瑞
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Guangzhou Sugarcane Industry Research Institute
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Guangzhou Sugarcane Industry Research Institute
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Application filed by Guangzhou Sugarcane Industry Research Institute filed Critical Guangzhou Sugarcane Industry Research Institute
Priority to CN201410351654.9A priority Critical patent/CN104341236A/en
Publication of CN104341236A publication Critical patent/CN104341236A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls

Abstract

The invention discloses a medium for cultivating pleurotus ostreatus by using bagasse. The medium comprises the following components by dry weight percent: 75%-80% of bagasse, 10%-20% of beer gains, 5%-10% of bran, 2%-5% of corn flour, 0.5%-1% of sugar, 1%-2% of lime and 0-1% of gypsum. Furthermore, a preparation method of the medium for cultivating pleurotus ostreatus by using bagasse comprises the following steps: (1) fully soaking the bagasse by virtue of water; (2) evenly mixing with the beer gains, the brain, the corn flour, the sugar, the lime and the gypsum form a medium; (3) enclosing the medium into a culture bag; and (4) sterilizing and cooling the culture bag with the medium for later use. According to the preparation and application methods of the medium for cultivating pleurotus ostreatus by using bagasse disclosed by the invention, the bagasse is utilized in a high-valued manner; the production cost of the pleurotus ostreatus is reduced; and meanwhile, the quality of the pleurotus ostreatus is improved.

Description

Bagasse is as the compound method of Oyster mushroom culture medium and use
Technical field
The present invention relates to a kind of oyster mushroom culture technique, particularly relate to a kind of bagasse that utilizes as the compound method of Oyster mushroom culture medium and use.
Background technology
Flat mushroom (Pleurotus ostreatus) have another name called pick up the ears, Fungus Pleurotus ostreatus etc., containing abundant nutritive ingredient, having well edible, pharmaceutical use, is that one of edible mushrooms is widely cultivated by China.For a long time mushroom cultivation mainly with cotton seed hull, wood chip, wheat straw, corn cob, other agricultural byproducts or tankage for main raw material.Forest tree resource is increasingly deficient in recent years, cotton seed hulls, corn cob are applied by many-side, and agricultural crop straw takes again also field measure, causes flat mushroom raw materials cost day by day to raise, had a strong impact on the economic benefit of mushroom cultivation, the novel material of exploitation cultivating white mushroom is imperative.
Bagasse is a large byproduct in sugar refinery, often produces the sucrose of one ton, will produce 2 ~ 3 tons of bagasses.Bagasse is primarily of Mierocrystalline cellulose, hemicellulose, xylogen and ash composition.By analysis, its content of cellulose is higher, accounts for 32%-48%, and ash oontent is lower, accounts for 1.5%-5%, and hemicellulose and xylogen respectively account for 19-24% and 23%-32%.From nutritive ingredient, bagasse may be used for edible fungus culturing, but also there are some problems, and as carbon source is enriched, but effectively nitrogenous source lacks, and pH is on the low side.At present, bagasse is mainly used in the raw material of boiler oil and other industry, its investment and working cost huge, the more important thing is the low and contaminate environment of the wasting of resources, economic benefit.
Summary of the invention
For solving above-mentioned existing technical disadvantages, higher value application bagasse, reduce the raw materials cost of mushroom cultivation, the invention provides a kind of utilize bagasse as Oyster mushroom culture medium and compound method and use.
For reaching above technical purpose, the technical solution used in the present invention is as follows:
Utilize bagasse as a matrix for mushroom cultivation, according to dry weight percentage, described cultivation matrix comprises: bagasse 75% ~ 80%, brewer's grains 10% ~ 20%, wheat bran 5% ~ 10%, Semen Maydis powder 2% ~ 5%, sugar 0.5% ~ 1%, lime 1% ~ 2%, gypsum 0% ~ 1%.
Preferably, described cultivation matrix comprises: bagasse 79%, brewer's grains 10%, wheat bran 5%, Semen Maydis powder 2%, sugar 1%, lime 2%, gypsum 1%.
Further, the aforesaid bagasse that utilizes is as the compound method of the matrix of mushroom cultivation, and it comprises the following steps:
(1) described bagasse water is fully soaked into;
(2) admix described brewer's grains, wheat bran, Semen Maydis powder, sugar, lime and gypsum, mix, form matrix;
(3) described matrix is loaded cultivating bag;
(4) the described cultivating bag that matrix is housed is carried out sterilizing, cool stand-by.
Further, described step (3) is further comprising the steps of:
When described matrix is loaded cultivating bag, in the middle of described matrix, remain for the duct accessing flat mushroom strain in advance.Preferably, the diameter in described duct is 1.5cm.
Water content about matrix: preferably, the water content of described matrix is 60% ~ 65%.
Further, the aforesaid bagasse that utilizes can also have other using method as the matrix of mushroom cultivation: the described matrix after flat mushroom of gathering is for the production of organic fertilizer.
Compared with prior art, the invention has the beneficial effects as follows:
(1) bagasse that utilizes provided by the present invention is as the matrix of mushroom cultivation, and nutritive props is reasonable, meets the nutritive element needed for each growth period of flat mushroom, the speed of growth of hypha of Pleurotus ostreatus can be accelerated, shorten the cultivation period of flat mushroom, and the flat mushroom quality produced is better;
(2) bagasse that utilizes provided by the present invention is as the matrix of mushroom cultivation, and the bagasse adopting low cost is one of component, effectively reduces the batching cost of this matrix;
(3) bagasse that utilizes provided by the present invention is as the matrix of mushroom cultivation, and after flat mushroom harvest, this matrix can also utilize further, as organic fertilizer after fermentation, can increase the added value that flat mushroom is produced;
(4) bagasse that utilizes provided by the present invention is as the compound method of the matrix of mushroom cultivation, simple to operate, is beneficial to reduction labor cost.
Accompanying drawing explanation
Fig. 1 is the mushroom growth situation schematic diagram utilizing the present invention to utilize bagasse to cultivate as the matrix of mushroom cultivation.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
One, the preparation of Oyster mushroom culture medium
Following material is taken: bagasse 79%, brewer's grains 10%, wheat bran 5%, Semen Maydis powder 2%, sugar 1%, lime 2%, gypsum 1% by dry matter percentage.Wherein, described brewer's grains at least should comprise crude protein 24.5%, crude fat 7.3%, robust fibre 13.8%, ash content 3.8%, calcium 0.3%, phosphorus 0.6%, potassium 0.08%, magnesium 0.14%, Methionin 0.9%, methionine(Met) 0.5%, Gelucystine 0.4%, arginine 1.5%, Isoleucine 1.4%, leucine 1.7%, phenylalanine 1.3%, tyrosine 1.3%, α-amino-isovaleric acid 1.6%.
Fully soaked into by bagasse water in above-mentioned material, spread out after picking up, other material is evenly spread on described bagasse surface, then mix, the water content control of mixture, 60% ~ 65%, piles fermentation in heaps 3 hours, forms matrix; The described matrix fermented is loaded in 17cm × 33cm acrylic plastering cultivating bag, in the middle of described cultivating bag, polyvinyl chloride pipe or the rod of a diameter 1.5cm is inserted during pack, take out after described matrix has packed, namely in matrix, form duct, this duct is for accessing flat mushroom strain.The cultivating bag installing described matrix needs to carry out autoclaving.
The dry weight percentage of each material of described Oyster mushroom culture medium can also adjust as follows:
Bagasse 75%, brewer's grains 14%, wheat bran 5%, Semen Maydis powder 2%, sugar 1%, lime 2%, gypsum 1%.
Two, the cultivation of flat mushroom
After described matrix cooling, access flat mushroom strain, be placed in darkroom and send out bacterium cultivation; Undertaken urging flower bud by controlling the temperature difference when hypha of Pleurotus ostreatus covers with cultivating bag; Then carry out management of producing mushroom, during fruiting, temperature controls at 22-26 DEG C, and atmospheric moisture is about 90%, keeps the ventilation of mushroom room, timely collecting flat mushroom.
Please refer to Fig. 1, the flat mushroom grown in described cultivation matrix, its Growth of Pleurotus Mycelium speed is accelerated, and cultivation period shortens, and the mushroom carpophore produced is delicate, and its aminoacids content is abundanter, thus nutritious, delicious flavour.
Three, the recycling of matrix
After flat mushroom fruiting three batches, described matrix will not be used further to mushroom cultivation; Carry out smashing stirring to pieces after when the time comes described matrix being poured out cultivating bag, add biological organic fertilizer bacterial classification and ferment, for as organic fertilizer after having fermented.

Claims (7)

1. one kind utilizes bagasse as the matrix of mushroom cultivation, it is characterized in that, according to dry weight percentage, described cultivation matrix comprises: bagasse 75% ~ 80%, brewer's grains 10% ~ 20%, wheat bran 5% ~ 10%, Semen Maydis powder 2% ~ 5%, sugar 0.5% ~ 1%, lime 1% ~ 2%, gypsum 0% ~ 1%.
2. utilize bagasse as the matrix of mushroom cultivation as claimed in claim 1, it is characterized in that, according to dry weight percentage, described cultivation matrix comprises: bagasse 79%, brewer's grains 10%, wheat bran 5%, Semen Maydis powder 2%, sugar 1%, lime 2%, gypsum 1%.
3. utilize bagasse as a compound method for the matrix of mushroom cultivation as claimed in claim 1 or 2, it is characterized in that, it comprises the following steps:
(1) described bagasse water is fully soaked into;
(2) admix described brewer's grains, wheat bran, Semen Maydis powder, sugar, lime and gypsum, mix, form matrix;
(3) described matrix is loaded cultivating bag;
(4) the described cultivating bag that matrix is housed is carried out sterilizing, cool stand-by.
4. utilize bagasse as the compound method of the matrix of mushroom cultivation as claimed in claim 3, it is characterized in that, described step (3) is further comprising the steps of:
When described matrix is loaded cultivating bag, in the middle of described matrix, remain for the duct accessing flat mushroom strain in advance.
5. utilize bagasse as the compound method of the matrix of mushroom cultivation as claimed in claim 4, it is characterized in that: the diameter in described duct is 1.5cm.
6. utilize bagasse as the compound method of the matrix of mushroom cultivation as claimed in claim 3, it is characterized in that: the water content of described matrix is 60% ~ 65%.
7. utilize bagasse as a using method for the matrix of mushroom cultivation as claimed in claim 1 or 2, it is characterized in that: the described matrix after flat mushroom of gathering is for the production of organic fertilizer.
CN201410351654.9A 2014-07-22 2014-07-22 Preparation method and application of pleurotus ostreatus culture medium by using bagasse Pending CN104341236A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105130626A (en) * 2015-08-27 2015-12-09 马鞍山市安康菌业有限公司 Pleurotus ostreatus culture medium for sugarcane scum with high biotransformation rate and preparation method therefor
CN105237147A (en) * 2015-09-01 2016-01-13 广西南宁胜祺安科技开发有限公司 Culture medium for oyster mushroom and preparation method thereof
CN107244947A (en) * 2017-02-27 2017-10-13 广东省生物工程研究所(广州甘蔗糖业研究所) The method that culture medium of edible fungus is made using cane sugar manufacture accessory substance continuous high-efficient

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Publication number Priority date Publication date Assignee Title
CN101514116A (en) * 2008-02-22 2009-08-26 大叶大学 Formula for culture medium for culturing mushrooms with bagasse
CN101953269A (en) * 2010-09-06 2011-01-26 阜阳市德益农业科技有限公司 Method and device for sealing edible fungi culture bag
CN102860413A (en) * 2012-09-28 2013-01-09 广州优锐生物科技有限公司 Nutritive feed and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101514116A (en) * 2008-02-22 2009-08-26 大叶大学 Formula for culture medium for culturing mushrooms with bagasse
CN101953269A (en) * 2010-09-06 2011-01-26 阜阳市德益农业科技有限公司 Method and device for sealing edible fungi culture bag
CN102860413A (en) * 2012-09-28 2013-01-09 广州优锐生物科技有限公司 Nutritive feed and preparation method thereof

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105130626A (en) * 2015-08-27 2015-12-09 马鞍山市安康菌业有限公司 Pleurotus ostreatus culture medium for sugarcane scum with high biotransformation rate and preparation method therefor
CN105237147A (en) * 2015-09-01 2016-01-13 广西南宁胜祺安科技开发有限公司 Culture medium for oyster mushroom and preparation method thereof
CN107244947A (en) * 2017-02-27 2017-10-13 广东省生物工程研究所(广州甘蔗糖业研究所) The method that culture medium of edible fungus is made using cane sugar manufacture accessory substance continuous high-efficient

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