CN112471325A - Straw biological fermentation feed and preparation method and application thereof - Google Patents
Straw biological fermentation feed and preparation method and application thereof Download PDFInfo
- Publication number
- CN112471325A CN112471325A CN202011439609.0A CN202011439609A CN112471325A CN 112471325 A CN112471325 A CN 112471325A CN 202011439609 A CN202011439609 A CN 202011439609A CN 112471325 A CN112471325 A CN 112471325A
- Authority
- CN
- China
- Prior art keywords
- parts
- preparation
- straw
- fermentation
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 71
- 230000004151 fermentation Effects 0.000 title claims abstract description 66
- 239000010902 straw Substances 0.000 title claims abstract description 65
- 238000002360 preparation method Methods 0.000 title claims abstract description 42
- 239000002131 composite material Substances 0.000 claims abstract description 33
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 241001465754 Metazoa Species 0.000 claims abstract description 19
- 239000003513 alkali Substances 0.000 claims abstract description 14
- 235000006533 astragalus Nutrition 0.000 claims abstract description 11
- 235000011194 food seasoning agent Nutrition 0.000 claims abstract description 11
- 235000013379 molasses Nutrition 0.000 claims abstract description 11
- 241000045403 Astragalus propinquus Species 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 54
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 36
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 22
- 241000186361 Actinobacteria <class> Species 0.000 claims description 18
- 239000004310 lactic acid Substances 0.000 claims description 18
- 235000014655 lactic acid Nutrition 0.000 claims description 18
- 230000000243 photosynthetic effect Effects 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 239000004278 EU approved seasoning Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 235000013406 prebiotics Nutrition 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 240000006439 Aspergillus oryzae Species 0.000 claims description 5
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 5
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 5
- 241000187392 Streptomyces griseus Species 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 4
- 102000013142 Amylases Human genes 0.000 claims description 4
- 235000001014 amino acid Nutrition 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 235000019418 amylase Nutrition 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 241000223261 Trichoderma viride Species 0.000 claims description 3
- 241000228245 Aspergillus niger Species 0.000 claims description 2
- 241001061264 Astragalus Species 0.000 claims description 2
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 2
- 241000187722 Micromonospora echinospora Species 0.000 claims description 2
- 108010059820 Polygalacturonase Proteins 0.000 claims description 2
- 241000191025 Rhodobacter Species 0.000 claims description 2
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims description 2
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 claims description 2
- 210000004233 talus Anatomy 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- 102000035195 Peptidases Human genes 0.000 claims 1
- 229940025131 amylases Drugs 0.000 claims 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims 1
- 150000003271 galactooligosaccharides Chemical class 0.000 claims 1
- 241001446247 uncultured actinomycete Species 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 10
- 230000036039 immunity Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000006870 function Effects 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 abstract description 4
- 230000000968 intestinal effect Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000009467 reduction Effects 0.000 abstract description 2
- 240000008042 Zea mays Species 0.000 description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 15
- 235000005822 corn Nutrition 0.000 description 15
- 239000000463 material Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 210000001035 gastrointestinal tract Anatomy 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 244000037364 Cinnamomum aromaticum Species 0.000 description 4
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 description 4
- -1 VB12) Natural products 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 235000019614 sour taste Nutrition 0.000 description 4
- 235000019605 sweet taste sensations Nutrition 0.000 description 4
- 239000004382 Amylase Substances 0.000 description 3
- 240000004760 Pimpinella anisum Species 0.000 description 3
- 235000012550 Pimpinella anisum Nutrition 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000000378 dietary effect Effects 0.000 description 3
- 239000000428 dust Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- CSOJYIADHHNGRM-UHFFFAOYSA-N 6-(4-oxo-1,3-thiazolidin-2-yl)hexanoic acid Chemical compound OC(=O)CCCCCC1NC(=O)CS1 CSOJYIADHHNGRM-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001346334 Amomum tsao-ko Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000159443 Myrcia Species 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 108010027322 single cell proteins Proteins 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- WMDVTEYZKOEFEB-UHFFFAOYSA-N 1-[2-(3,5-dihydroxyphenyl)-6-hydroxy-5-methoxy-1-benzofuran-4-yl]-3-methylbutan-2-one Chemical compound C=1C2=C(CC(=O)C(C)C)C(OC)=C(O)C=C2OC=1C1=CC(O)=CC(O)=C1 WMDVTEYZKOEFEB-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 235000007650 Aralia spinosa Nutrition 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108010029541 Laccase Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 244000270834 Myristica fragrans Species 0.000 description 1
- 235000009421 Myristica fragrans Nutrition 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000191996 Pediococcus pentosaceus Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N Xylose Natural products O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 241000949456 Zanthoxylum Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
- 229940107187 fructooligosaccharide Drugs 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- HDRZRQNZNIJSIQ-UHFFFAOYSA-N moracin Q Natural products COC1=CC(OC)=CC(C=2OC3=CC=4OC(C)(C)C(O)CC=4C=C3C=2)=C1 HDRZRQNZNIJSIQ-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001702 nutmeg Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/517—Bifidum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a straw biological fermentation feed and a preparation method and application thereof. The preparation method of the straw biological fermentation feed adopts the composite microecological preparation to ferment the raw materials containing the straw; wherein the raw materials comprise the following components in parts by weight: 65-75 parts of straw, 15-25 parts of molasses, 2-5 parts of astragalus membranaceus, 2-5 parts of seasoning and 0.5-5 parts of alkali. The straw biological fermentation feed can strengthen the functions of beneficial microorganisms in the animal body, regulate the ecological balance of intestinal microorganisms, enhance the immunity of the animal, improve the growth performance, the survival rate and the relative growth rate of the animal, reduce the morbidity of the animal, and has the advantages of cost reduction, efficiency improvement, income improvement and the like.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a straw biological fermentation feed and a preparation method and application thereof.
Background
In a broad sense, the parts of crops such as corn, wheat, rice, etc. except for the grains and roots can be collectively referred to as "straw". The problem of straw burning in rural areas of China has been long, and the straw burning brings serious troubles to the normal operation of life and economy of people, and becomes a long-standing problem in society. The government has no burning measures for the frequent straw burning phenomenon in various regions, but the effect is very little. The residual amount of the straws in agricultural production is very large, environmental pollution is caused by burning in a conventional treatment method, the conversion rate of the nutrition value of the primary feed processing is not high, and waste and high culture cost are caused.
The Chinese patent application with publication number CN110353087A discloses a preparation method of rape straw fermented feed and the fermented feed, comprising the following steps: 1) drying in the sun, cutting and crushing; 2) dissolving glucose and urea in water, and uniformly spraying the solution on rape straws; 3) dissolving cellulase and laccase in water, and uniformly spraying the solution on rape straws; 4) spraying a probiotic starter on the rape straws, wherein the probiotic starter consists of trichoderma viride, candida utilis and pediococcus pentosaceus; 5) and (2) putting the rape straws into a fermentation barrel, compacting at high density, wrapping with a stretching film, placing in a flat and dry room, and naturally fermenting at room temperature for 20 days to complete the preparation of the rape straw fermented feed.
Chinese patent application with publication number CN105941825A discloses a corn straw biological fermentation feed and a preparation method thereof, comprising the following steps: 1) crushing the corn straws, and sieving the crushed corn straws with a sieve of 20-40 meshes to obtain corn straw powder; spraying ammonia water on the corn stalk powder, turning over the corn stalk powder while spraying, stacking for 8-10 days, and steaming at high pressure; 2) preparing fermentation culture solution containing 0.2-0.4% of dipotassium phosphate, 0.1-0.2% of monopotassium phosphate, 0.1-0.2% of urea, 0.04-0.06% of magnesium sulfate, 4-6% of ammonium sulfate and 0.04-0.06% of calcium chloride, adjusting the pH value to 5.0-6.0, and sterilizing at the temperature of 100 ℃ and 120 ℃ for 10-20 minutes; 3) uniformly mixing the corn straw powder and the fermentation culture solution according to the ratio of 1:40-50, adding a compound Moracin Q, inoculating bacillus licheniformis, fermenting and culturing for 3-5 days, and thus obtaining the corn straw biological fermentation feed.
Most of the existing straw fermented feeds have the problems of high cost, single effect, poor effect and the like; in view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a straw biological fermentation feed, a preparation method and an application thereof, and the straw biological fermentation feed can mediate the ecological balance of intestinal microorganisms, enhance the animal immunity, improve the growth performance, the survival rate and the relative growth rate of animals, and reduce the morbidity.
The invention provides a preparation method of straw biological fermentation feed, which adopts a composite microecological preparation to ferment raw materials containing straws; wherein the raw materials comprise the following components in parts by weight: 65-75 parts of straw, 15-25 parts of molasses, 2-5 parts of astragalus membranaceus, 2-5 parts of seasoning and 0.5-5 parts of alkali.
The compound microecological preparation adopted by the invention comprises lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis; wherein, in each unit of the composite microecological preparation, the mass ratio of lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis is (4-6): (1-2): (1-2): (2-3): (3-5); in particular, the effective viable count of each unit of the composite microecological preparation is more than or equal to 50 hundred million.
The lactobacillus can produce organic acid, special enzyme system, acidomycin and other substances through fermentation, and has the physiological functions of promoting animal growth, regulating normal flora in gastrointestinal tract, maintaining microecological balance, improving gastrointestinal tract function, increasing food digestibility and biological value, reducing serum cholesterol, controlling endotoxin, inhibiting putrefying bacteria growth in intestinal tract, improving organism immunity and the like.
In particular, the lactic acid bacteria of the present invention may be selected from at least one of lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium bifidum, preferably lactobacillus plantarum subspecies ficc 20272, which is capable of producing exopolysaccharides, and is significantly effective when used in combination with several other microorganisms.
The photosynthetic bacteria can perform photosynthesis by taking light as an energy source and taking organic matters, sulfides, ammonia and the like in the nature as a hydrogen donor and carbon source under anaerobic illumination or aerobic dark conditions, separate hydrogen sulfide and hydrogen in hydrocarbons in the environment and change harmful substances into harmless substances; meanwhile, the thallus contains more than 60% of protein, the protein amino acid composition is complete, the cells also contain a plurality of vitamins, especially B vitamins are very rich, the content of VB2, folic acid, pantothenic acid and biotin is also high, and the thallus also contains a large amount of physiological active substances such as carotenoid and coenzyme Q, has high nutritive value and is the main power for promoting the growth of animals and plants.
Specifically, the photosynthetic bacteria of the present invention may be selected from at least one of rhodopseudomonas palustris and rhodobacter vannamei, preferably rhodopseudomonas palustris CICC 23812, which has rich nutrition, high protein content and rich bioactive substances.
Actinomycetes can directly inhibit pathogenic bacteria by producing various antibiotics, various enzyme preparations (e.g., protease, amylase, cellulase, etc.), vitamins (e.g., VB12), various organic acids, and the like by fermentation.
Specifically, the actinomycetes of the invention can be selected from at least one of streptomyces griseus and micromonospora purpurea, preferably streptomyces griseus CICC 11002, which can produce streptomycin and further inhibit various pathogenic bacteria.
The filamentous fungi of fermentation system can coexist with other microorganisms, has a good effect particularly on the formation of esters in soil, has a strong alcohol-forming ability, can prevent the occurrence of pests, and can eliminate offensive odors.
Specifically, the filamentous fungus of the fermentation system of the present invention may be at least one selected from aspergillus niger, aspergillus oryzae and trichoderma viride, preferably aspergillus oryzae cic 2011, which is capable of producing a biological enzyme such as neutral protease and has activities such as saccharifying enzyme, amylase, transglucosidase, pullulanase and the like.
The candida utilis can synthesize protein with rich nutrition by utilizing various hexose, pentose and urea or cheap industrial and agricultural byproducts, waste materials and the like, the content of the protein and vitamin B of the candida utilis exceeds that of saccharomyces cerevisiae, and the candida utilis is an excellent strain for producing single-cell protein for eating, medicine or feed; meanwhile, the candida utilis can also provide important nutrition guarantee for substrates required by promoting the proliferation of other microorganisms (such as lactic acid bacteria and actinomycetes), so that good mutual cooperation and promotion effects are formed. Preferably, the present invention can employ candida utilis cic 1769, which is capable of producing high quality edible single cell proteins.
Further, the composite microecological preparation may further include at least one of prebiotics, biological enzymes, proteins, amino acids, trace elements, and minerals; can be reasonably assembled according to actual requirements.
The prebiotics can promote the growth and reproduction of beneficial bacteria in the intestinal tract in vivo, form microecological competitive advantages, optimize the microecological balance of the intestinal tract, further improve the immunity, keep the body healthy and improve the microecological of the intestinal tract. Specifically, the prebiotics may be at least one selected from the group consisting of fructo-oligosaccharide, xylose oligosaccharide, galactose oligosaccharide and isomalto-oligosaccharide, and the amount of the prebiotics may be 1% to 2%.
The biological enzyme can degrade macromolecular substances such as protein, fat, polysaccharide and the like into small molecular substances which are easy to absorb, such as oligopeptide, amino acid, fatty acid, glucose and the like, so that the digestion and absorption of nutrients are promoted, the growth rate and the feed conversion rate of animals are improved, the health of the animals is improved, the emission of nitrogen and phosphorus in the sewage discharge of the breeding industry can be reduced, and the ecological environment is protected. Specifically, the biological enzyme of the present invention may be at least one selected from the group consisting of amylase, protease, xylanase and pectinase, and the amount of the biological enzyme may be 3% to 5%.
The above-mentioned bacteria can be purchased from China center for Industrial culture Collection of microorganisms (CICC). The composite microecological preparation has sour and sweet taste and fermentation flavor, has effective viable count of more than or equal to 50 hundred million, has the characteristics of high bacterial count, complete functions, high activity, good effect, long lasting effect and the like, and can be widely applied to the fields of breeding industry, planting industry, environmental protection and the like.
The flavoring and alkali used in the invention are not restricted strictly, the flavoring can be one or more of pricklyash peel, aniseed, cassia bark, nutmeg, bay leaf and the like, and the alkali can be edible alkali, baking soda and the like.
More specifically, the preparation method of the straw biological fermentation feed comprises the following steps:
A) crushing, sieving and secondarily crushing the straws, and then stirring and mixing the straws with astragalus and seasonings to prepare a fermentation raw material;
B) dissolving the composite microecological preparation, molasses and alkali in water, spraying onto the fermentation raw material, and fermenting.
Wherein, during fermentation, the dosage of the composite microecologics adopted by each ton of straws is 1-2 kg; the fermentation may include: anaerobic fermentation is carried out for 68-76h at the temperature of 20-35 ℃.
The invention also provides a straw biological fermentation feed which is prepared according to the preparation method.
The invention also provides application of the straw biological fermentation feed in animal feeding.
The straw biological fermentation feed disclosed by the invention at least has the following advantages:
1. according to the straw biological fermentation feed, the straw and other raw materials are fermented and decomposed by adding the composite microbial ecological agent, so that the utilization rate of the straw feed is improved;
2. the straw biological fermentation feed can strengthen the functions of beneficial microorganisms in the animal body and regulate the ecological balance of intestinal microorganisms;
3. the straw biological fermentation feed can enhance the animal immunity and improve the resistance to diseases;
4. the straw biological fermentation feed can improve the growth performance of animals, beneficial bacteria and fermentation products thereof improve the digestibility of protein and other nutrient substances in the feed, and has the obvious advantages of cost reduction, efficiency improvement, yield improvement and the like;
5. the straw biological fermentation feed can improve the environment quality of a farm, improve the survival rate and the relative growth rate of animals, and reduce the morbidity.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms also include the plural forms unless the context clearly dictates otherwise, and further, it is understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, devices, components, and/or combinations thereof.
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The species sources used in the examples are as follows:
lactic acid bacteria: lactobacillus plantarum subspecies plantaris CICC 20272;
photosynthetic bacteria: rhodopseudomonas palustris CICC 23812;
actinomycetes: streptomyces griseus CICC 11002;
fermentation-line filamentous fungi: aspergillus oryzae CICC 2011;
candida utilis: candida utilis cic 1769;
all the above-mentioned bacteria are purchased from China center for Industrial culture Collection of microorganisms (CICC).
Example 1
Firstly, preparing the composite microecological preparation
The composite microecological preparation of the embodiment comprises lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis; wherein the mass ratio of the lactic acid bacteria, the photosynthetic bacteria, the actinomycetes, the fermentation system filamentous bacteria and the candida utilis is 6: 1: 2: 2: 4.
according to the weight parts, lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation series filamentous bacteria and candida utilis are uniformly mixed to prepare the composite microecological preparation, the composite microecological preparation has sour and sweet taste and fermentation flavor, and the effective viable count is more than or equal to 50 hundred million/g.
Secondly, fermentation
The composition of the raw materials of this example is as follows: 70 parts of corn straw, 20 parts of molasses, 3 parts of astragalus membranaceus, 3 parts of seasonings (comprising 1 part of each of cassia bark, amomum tsao-ko and myrcia), and 2.5 parts of dietary alkali; the dosage of the composite microecologics adopted by each ton of straws is 1.5 kg.
Crushing large bundles of corn straws, sieving to remove impurities, then performing secondary crushing, selecting the number of crushed materials according to different animals to be fed, wherein the size of a screw hole is 8-20, performing secondary crushing, conveying the crushed materials into a conveyor belt through a dust outlet fan, stirring the crushed materials in a TM machine, automatically weighing, adding the astragalus membranaceus and seasonings according to parts by weight, and mixing to obtain the fermentation raw material.
And (3) dissolving the composite microbial ecological agent, molasses and alkali in water according to the above dosage, spraying the mixture onto the fermentation raw materials, carrying out anaerobic fermentation at about 35 ℃ for 72 hours, and standing at normal temperature for 7 days to obtain the straw biological fermentation feed.
Example 2
Firstly, preparing the composite microecological preparation
The composite microecological preparation of the embodiment comprises lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis; wherein the mass ratio of the lactic acid bacteria, the photosynthetic bacteria, the actinomycetes, the fermentation system filamentous bacteria and the candida utilis is 4: 2: 1: 3: 3.
according to the weight parts, lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation series filamentous bacteria and candida utilis are uniformly mixed to prepare the composite microecological preparation, the composite microecological preparation has sour and sweet taste and fermentation flavor, and the effective viable count is more than or equal to 50 hundred million/g.
Secondly, fermentation
The composition of the raw materials of this example is as follows: 75 parts of corn straw, 15 parts of molasses, 5 parts of astragalus membranaceus, 5 parts of seasonings (comprising 1 part of aniseed, cassia bark, amomum tsao-ko and myrcia) and 4 parts of dietary alkali; the dosage of the composite microecologics adopted by each ton of straws is 1 kg.
Crushing large bundles of corn straws, sieving to remove impurities, then performing secondary crushing, selecting the number of crushed materials according to different animals to be fed, wherein the size of a screw hole is 8-20, performing secondary crushing, conveying the crushed materials into a conveyor belt through a dust outlet fan, stirring the crushed materials in a TM machine, automatically weighing, adding the astragalus membranaceus and seasonings according to parts by weight, and mixing to obtain the fermentation raw material.
And (3) dissolving the composite microbial ecological agent, molasses and alkali in water according to the above dosage, spraying the mixture onto the fermentation raw materials, carrying out anaerobic fermentation at about 32 ℃ for 72 hours, and standing at normal temperature for 7 days to obtain the straw biological fermentation feed.
Example 3
Firstly, preparing the composite microecological preparation
The composite microecological preparation of the embodiment comprises lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis; wherein the mass ratio of the lactic acid bacteria, the photosynthetic bacteria, the actinomycetes, the fermentation system filamentous bacteria and the candida utilis is 5: 1: 1: 2: 5.
according to the weight parts, lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation series filamentous bacteria and candida utilis are uniformly mixed to prepare the composite microecological preparation, the composite microecological preparation has sour and sweet taste and fermentation flavor, and the effective viable count is more than or equal to 50 hundred million/g.
Secondly, fermentation
The composition of the raw materials of this example is as follows: 65 parts of corn straw, 25 parts of molasses, 2 parts of astragalus membranaceus, 2 parts of seasonings (comprising 1 part of aniseed and 1 part of cassia bark) and 1 part of dietary alkali; the dosage of the composite microecologics adopted by each ton of straws is 2 kilograms.
Crushing large bundles of corn straws, sieving to remove impurities, then performing secondary crushing, selecting the number of crushed materials according to different animals to be fed, wherein the size of a screw hole is 8-20, performing secondary crushing, conveying the crushed materials into a conveyor belt through a dust outlet fan, stirring the crushed materials in a TM machine, automatically weighing, adding the astragalus membranaceus and seasonings according to parts by weight, and mixing to obtain the fermentation raw material.
And (3) dissolving the composite microbial ecological agent, molasses and alkali in water according to the above dosage, spraying the mixture onto the fermentation raw materials, carrying out anaerobic fermentation at about 35 ℃ for 72 hours, and standing at normal temperature for 7 days to obtain the straw biological fermentation feed.
Comparative example 1
In the comparative example, the mass ratio of lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis in the composite microecological preparation is 1: 1: 1: 1: except for 1, the same procedure as in example 1 was repeated.
Comparative example 2
This comparative example was the same as example 1 except that in the composite microecological formulation, lactobacillus plantarum subspecies fic 20038 was used instead of lactobacillus plantarum subspecies fic 20272 of example 1.
Comparative example 3
Adding the compound microecological preparation of the example 1 into a conventional feed, adding 1.5 kg of the compound microecological preparation per ton of the feed, and uniformly stirring (not fermenting) to obtain the biological feed.
Test example 1
The feed of examples 1 to 3 and comparative examples 1 to 3 were used, respectively, and fed by a conventional feeding method, and the results of the growth performance of the pigs are shown in Table 1.
TABLE 1 growth Performance results in pigs
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A preparation method of straw biological fermentation feed is characterized by comprising the following steps: fermenting the raw material containing the straws by adopting a composite microecological preparation; wherein the raw materials comprise the following components in parts by weight: 65-75 parts of straw, 15-25 parts of molasses, 2-5 parts of astragalus membranaceus, 2-5 parts of seasoning and 0.5-5 parts of alkali.
2. The method of claim 1, wherein the complex microbial ecological agent comprises lactic acid bacteria, photosynthetic bacteria, actinomycetes, filamentous fungi of fermentation system, and candida utilis; wherein, in each unit of the composite microecological preparation, the mass ratio of lactic acid bacteria, photosynthetic bacteria, actinomycetes, fermentation system filamentous bacteria and candida utilis is (4-6): (1-2): (1-2): (2-3): (3-5).
3. The method according to claim 2, wherein the lactic acid bacteria are selected from at least one of Lactobacillus acidophilus, Lactobacillus plantarum and Bifidobacterium bifidum, preferably Lactobacillus plantarum subspecies CICC 20272; the photosynthetic bacteria are selected from at least one of rhodopseudomonas palustris and rhodobacter vannamei, preferably rhodopseudomonas palustris CICC 23812; the actinomycete is selected from at least one of streptomyces griseus and micromonospora purpurea, preferably streptomyces griseus CICC 11002; the fermentation filamentous fungi is at least one of aspergillus niger, aspergillus oryzae and trichoderma viride, and is preferably aspergillus oryzae CICC 2011.
4. The preparation method according to claim 2, wherein the complex microecological formulation further comprises at least one of prebiotics, biological enzymes, proteins, amino acids, trace elements and minerals, preferably prebiotics and/or biological enzymes, wherein the prebiotics are selected from at least one of fructo-oligosaccharides, xylo-oligosaccharides, galacto-oligosaccharides and isomalto-oligosaccharides, and the biological enzymes are selected from at least one of amylases, proteases, xylanases and pectinases.
5. The preparation method according to claim 4, wherein the prebiotics are added in an amount of 1% -2% and the biological enzymes are added in an amount of 3% -5%.
6. The method of claim 1, comprising the steps of:
A) crushing, sieving and secondarily crushing the straws, and then stirring and mixing the straws with astragalus and seasonings to prepare a fermentation raw material;
B) dissolving the composite microecological preparation, molasses and alkali in water, spraying onto the fermentation raw material, and fermenting.
7. The method according to claim 1, wherein the amount of the composite microecological agent used per ton of straw is 1-2 kg.
8. The method of claim 1, wherein the fermenting comprises: anaerobic fermentation is carried out for 68-76h at the temperature of 20-35 ℃.
9. A straw bio-fermented feed, which is prepared by the preparation method of any one of claims 1 to 8.
10. Use of the straw biofermented feed according to claim 9 in animal feeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011439609.0A CN112471325A (en) | 2020-12-11 | 2020-12-11 | Straw biological fermentation feed and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011439609.0A CN112471325A (en) | 2020-12-11 | 2020-12-11 | Straw biological fermentation feed and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112471325A true CN112471325A (en) | 2021-03-12 |
Family
ID=74941441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011439609.0A Pending CN112471325A (en) | 2020-12-11 | 2020-12-11 | Straw biological fermentation feed and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112471325A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151012A (en) * | 2021-05-11 | 2021-07-23 | 河北大河生物科技有限公司 | High-density fermentation method of antrodia camphorata |
| CN114903118A (en) * | 2022-05-17 | 2022-08-16 | 广西富鹏农牧有限公司 | Feed leavening agent and application thereof |
| CN117530365A (en) * | 2023-11-09 | 2024-02-09 | 湖北润泛生物科技有限公司 | Cellulose source feed for strawberry tea branches and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3937845A (en) * | 1975-01-08 | 1976-02-10 | The United States Of America As Represented By The Secretary Of Agriculture | Semi-solid fermentation of straw |
| CN101173211A (en) * | 2007-09-27 | 2008-05-07 | 顾建洪 | Composite type microecology preparation |
| CN105002116A (en) * | 2015-07-29 | 2015-10-28 | 河南省金阳生态工程有限公司 | Microorganism straw feed microbial agent and preparation method thereof |
| CN105981916A (en) * | 2015-02-17 | 2016-10-05 | 吉林省农业科学院 | Biological preparation used for straw feed |
| CN107041472A (en) * | 2017-06-15 | 2017-08-15 | 史占彪 | The preparation method of blue or green wheat stalk feed |
| CN107125460A (en) * | 2017-06-15 | 2017-09-05 | 史占彪 | The preparation method of green corn stover stalk feed |
| CN110477190A (en) * | 2019-08-28 | 2019-11-22 | 上海交通大学 | A kind of stalk anaerobic fermentation feed and preparation process and formula forage |
-
2020
- 2020-12-11 CN CN202011439609.0A patent/CN112471325A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3937845A (en) * | 1975-01-08 | 1976-02-10 | The United States Of America As Represented By The Secretary Of Agriculture | Semi-solid fermentation of straw |
| CN101173211A (en) * | 2007-09-27 | 2008-05-07 | 顾建洪 | Composite type microecology preparation |
| CN105981916A (en) * | 2015-02-17 | 2016-10-05 | 吉林省农业科学院 | Biological preparation used for straw feed |
| CN105002116A (en) * | 2015-07-29 | 2015-10-28 | 河南省金阳生态工程有限公司 | Microorganism straw feed microbial agent and preparation method thereof |
| CN107041472A (en) * | 2017-06-15 | 2017-08-15 | 史占彪 | The preparation method of blue or green wheat stalk feed |
| CN107125460A (en) * | 2017-06-15 | 2017-09-05 | 史占彪 | The preparation method of green corn stover stalk feed |
| CN110477190A (en) * | 2019-08-28 | 2019-11-22 | 上海交通大学 | A kind of stalk anaerobic fermentation feed and preparation process and formula forage |
Non-Patent Citations (1)
| Title |
|---|
| 张梅花主编: "《 新型农民阳光培训教材 农作物秸秆综合利用技术》", 28 February 2013, 科学普及出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151012A (en) * | 2021-05-11 | 2021-07-23 | 河北大河生物科技有限公司 | High-density fermentation method of antrodia camphorata |
| CN114903118A (en) * | 2022-05-17 | 2022-08-16 | 广西富鹏农牧有限公司 | Feed leavening agent and application thereof |
| CN117530365A (en) * | 2023-11-09 | 2024-02-09 | 湖北润泛生物科技有限公司 | Cellulose source feed for strawberry tea branches and preparation method thereof |
| CN117530365B (en) * | 2023-11-09 | 2024-05-17 | 湖北润泛生物科技有限公司 | Cellulose source feed for strawberry tea branches and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101401614B (en) | Multi-bacterial fermentation production of protein feedstuff with steam exploration of vegetables dregs and cotton dregs and method thereof | |
| CN102715339B (en) | Microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues | |
| CN101390566B (en) | Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff | |
| CN110122658A (en) | A kind of fermentative feedstuff of microbe and its preparation method and application | |
| CN110384175B (en) | Method for preparing yeast culture by using vinasse and application of yeast culture | |
| CN102696860B (en) | Highly efficient and low-cost microbiological feed proteins based on vinegar residue and miscellaneous meal | |
| CN112471325A (en) | Straw biological fermentation feed and preparation method and application thereof | |
| CN105076753A (en) | Method for preparing cattle and sheep feed by fermenting bagasse | |
| CN101107970A (en) | Bion cow concentrated feed and method for making same | |
| CN106858066A (en) | Collaboration promotes the additive and application method of proliferation of intestinal probiotics and field planting | |
| CN104664154B (en) | Yeast culture and preparation method thereof | |
| CN103355472A (en) | Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof | |
| CN106173204B (en) | Method for preparing high-protein feed by fermenting citric acid corn starch residues and hypha residues serving as base materials | |
| CN105002116A (en) | Microorganism straw feed microbial agent and preparation method thereof | |
| CN109007280A (en) | A kind of biofermentation type function bighead feed | |
| CN110973355A (en) | Whole-plant hybrid broussonetia papyrifera biological feed leavening agent | |
| CN110403073A (en) | A kind of soybean stalk fermented feed and preparation method thereof | |
| CN113974013A (en) | Fungus chaff fermented feed applied to laying hen breeding | |
| CN112385736A (en) | Method for fermenting oil bran by virtue of cooperation of bacterial enzymes | |
| CN102669408A (en) | Method for producing feed through biologic improvement on compound grains | |
| KR20180075986A (en) | A Method for functional fermentation composition | |
| CN101219154A (en) | Microorganism additive agent for improving phylactic power of pets and accelerating growth | |
| CN106135677A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with manioc waste | |
| KR101252134B1 (en) | Feed additives for promoting growth of cattle and process for the preparation of feed for breeding cattle using the same | |
| CN114946997A (en) | Method for producing compound feed by fermenting mixed strains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20211207 Address after: 101100 No. 6244, building 6, No. 17, Yunshan South Road, Industrial Development Zone, Tongzhou District, Beijing Applicant after: Beijing Haomiao Biotechnology Group Co.,Ltd. Address before: 100020 102, 1-3 / F, building 2, yard 6, Jiuwen Road, Chaoyang District, Beijing Applicant before: Beijing haoyao Agricultural Technology Development Co.,Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210312 |