CN106858066A - Collaboration promotes the additive and application method of proliferation of intestinal probiotics and field planting - Google Patents
Collaboration promotes the additive and application method of proliferation of intestinal probiotics and field planting Download PDFInfo
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Abstract
A kind of to cooperate with the additive and application method for promoting proliferation of intestinal probiotics and field planting, it is made up of xylo-oligosaccharide, clostridium butyricum and fulvic acid sodium humate high, and xylo-oligosaccharide provides nutrition for clostridium butyricum, promotes clostridium butyricum in the propagation of enteron aisle；Clostridium butyricum secretion vitamin and growth factor etc. promote proliferation of probiotics, while organic acid and antibacterial peptide of secretion etc. suppress harmful bacteria growing；Fulvic acid sodium humate high is damaged by repairing intestinal mucosa, improve intestinal mucosal surface probiotics adhesin receptor level, and then promote probiotics in the field planting of enteron aisle, three passes through PROCESS COUPLING and function synergic, finally realize animal and bird intestines microecological balance and improve gut barrier and absorption function, reduce diarrhea rate and improve efficiency of feed utilization, and then reduce dependence of the breeding process to antibiotic.
Description
Technical field
Promote Additive Product of the proliferation of intestinal probiotics with field planting and its application the present invention relates to a kind of collaboration, specifically relate to
And one kind is with xylo-oligosaccharide, fulvic acid sodium humate high and clostridium butyricum as raw material, coupling mechanism is cooperateed with using three, promoted
Enter Additive Product of the proliferation of intestinal probiotics with field planting and its application in livestock and poultry cultivation.
Background technology
It is more common method using antibiotic preventing and treating livestock and poultry intestinal canal diseases with the development of intensive culture, but it is anti-
Abusing and abusing for raw element, causes drug resistance problems to highlight, and there is security threat to human health.Exploitation is applied to animal and bird intestines
With the new additive agent of micro-ecological environment, while promoting intestinal absorption ability, reducing intestines problem generation, it is right to gradually reduce
The dependence of antibiotic, is the R＆D direction of following additive.
Xylo-oligosaccharide can be widely applied to food and field of fodder, be generally acknowledged functional oligose.Xylo-oligosaccharide is being returned
Intestines, caecum, colon can widely be fermented utilization by microorganism, by selectively promoting beneficial bacterium（Such as clostridium butyricum）It is raw
It is long, suppress harmful bacteria increment, and then improve enteric microorganism structure, promote intestinal microecology balance.
Clostridium butyricum is Gram-positive anaerobic spore-bearing bacilli, and clostridium butyricum is the beneficial of regulating intestinal canal microecological balance
Bacterium, its primary biological characteristic shows as：1. intestinal beneficial flora is promoted to breed by secreting vitamin and growth factor etc., while
Suppress harmful bacteria breeding in enteron aisle by secreting butyric acid and antibacterial peptide etc., reduce the secretion of enterotoxin；2. clostridium butyricum is main
Metabolite butyric acid provides nutrition for gut epithelium histocyte, promotes intestinal epithelial cell development；3. clostridium butyricum is anaerobism
Bacillus, can tolerate the high temperature such as feed granulating, drying and oxygen-enriched environment, it is ensured that the number of viable of clostridium butyricum in feed,
It is resistant to hydrochloric acid in gastric juice, bile acid etc. simultaneously, it is ensured that viable bacteria smoothly enters enteron aisle, plays prebiotic function.
Humic acid composition can be divided into fulvic acid, ulmic acid and humic acid three according to physics and chemistry and molecular machinery in sodium humate
Kind.Fulvic acid contained therein has remarkable effect for the regeneration of damaged intestine position capillary.Due to mineral resources humic acid
Fulvic acid content is generally lower than 1% in sodium, greatly reduces the clinical physiological effect of sodium humate.The microorganisms such as whiterot fungi point
The metabolite secreted can degrade the part branched structure in humic acid, discharge the smaller fulvic acid structural material of molecular weight,
And then improve fulvic acid content in sodium humate.
The related additive producing method method of various Tiny ecosystems is disclosed at present, and patent 201410137978.2 is disclosed
A kind of clostridium butyricum and its application, by the use of one or more in maltodextrin, wheat bran, dregs of beans, stone flour as carrier, in carrier
Material can not for clostridium butyricum growth nutrition is provided.Patent 201410702431.2 discloses a kind of compound micro-ecological preparation,
Various probiotics and oligosaccharide are wherein included, wherein oligosaccharide can promote the propagation of probiotics, but probiotics cannot be existed
Field planting in enteron aisle is intervened, and greatly reduces effect of probiotics.Patent 201310515953.7 discloses a kind of rare earth
With sodium humate composite feed additive and preparation method thereof, simply simply by sodium humate added in feed, emphasis is strong
The research of the production technology of sodium humate, application method and mechanism of the shortage to it in feed etc. is adjusted.As it was previously stated, oligomeric
The method and preparation that xylose, clostridium butyricum and fulvic acid sodium humate three high synergy improve intestinal health are rarely reported.
The content of the invention
Synchronously promote proliferation of intestinal probiotics and be colonized and suppress harmful bacteria to breed it is an object of the invention to provide a kind of,
And then ensure the additive of intestinal microecology balance.The advantage that the additive is different from general probiotics is, said preparation
Nutriment xylo-oligosaccharide comprising probiotics, the clostridium butyricum that suppression harmful bacteria proliferation activity material can be secreted and reparation intestines
Road epithelial damage, improves the fulvic acid sodium humate high of epithelium probiotics adhesion receptors level.Three kinds of materials pass through mechanism
Collaboration and Function Coupling, promote the sound development of intestinal microecology, and then promote gut barrier and absorption function, improve feed profit
With rate, promote growth of animals or poultry.
It is a further object to provide a kind of Additive Product for cooperateing with and promoting proliferation of intestinal probiotics and field planting
Application method.
The object of the present invention is achieved like this：It is a kind of to cooperate with the additive for promoting proliferation of intestinal probiotics and field planting, it
It is made up of following raw materials in parts by weight：Xylo-oligosaccharide 10-15 parts, clostridium butyricum 10-15 parts, fulvic acid sodium humate high
70-80 parts.
The specific feature of this programme also has, and the xylo-oligosaccharide refers to the wherein degree of polymerization for the xylo-oligosaccharide content of 2-4 is
More than 55%（Product is produced by Shandong Longli Biology Science and Technology Co., Ltd）.
Fulvic acid content >=12% in the fulvic acid sodium humate high.
Fulvic acid sodium humate high is to be made by the steps to form：
（1）By Phanerochaete chrysosporium（Purchased from Chinese industrial Microbiological Culture Collection administrative center, numbering CICC 40299,Phanerochaete chrysosporium）It is inoculated into malt juice liquid medium, is made after being cultivated 4-8 days in 39 degrees Celsius
Standby mycelia liquid.
（2）By mycelia liquid, pure water and sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces）According to weight 1-
3：1-2：1-4 mixes, and is that radix addition inorganic salts are specifically included by total weight of the mixture after mixing：Urea 0.1-0.3%, phosphoric acid
Hydrogen dipotassium 0.1-0.5%, potassium dihydrogen phosphate 0.1-0.5%, magnesium sulfate 0.1-0.3%, manganese sulfate 0.01-0.05%.
（3）PH is controlled for 4.8-6.0, temperature is 30-37 degrees Celsius, 60 revs/min of stirring reactions 5-10 days.
（4）By step（3）The reaction solution of acquisition uses roller drying, obtains fulvic acid sodium humate sheet products high.
Described clostridium butyricum active bacteria number is 1010-1011Bacterium colony/gram.
Clostridium butyricum is to be made by the steps to form：
（1）Butyrate spindle bacillus seed nutrient solution is made, wherein bacterial concentration is 5.0 × 108CFU/ml；
（2）Culture medium is made, specially：High temperature beancake powder 1.8%, urea 0.1%, corn pulp 0.1%, xylo-oligosaccharide 3.0%, phosphoric acid
Hydrogen dipotassium 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%；
（3）Butyrate spindle bacillus seed liquid is added in culture medium in 2% ratio using peristaltic pump, 35 DEG C of quiescent cultures 28 hours；
With xylo-oligosaccharide as sole carbon source in clostridium butyricum fermentation process；Sampling detection total viable count, total viable count be 2.7 ×
109CFU/ml, gemma rate is 100%；
（4）Zymotic fluid is centrifuged using tube centrifuge, carrier corncob, the aeration-drying below 40 DEG C, moisture control is added
5%, 100 mesh steel sieve was crushed, obtained clostridium butyricum.
After detection is qualified, packaging and storage.
A kind of foregoing collaboration promotes the application method of proliferation of intestinal probiotics and the additive of field planting, xylo-oligosaccharide
Raw material components I are mixed and made into clostridium butyricum；Fulvic acid sodium humate high is raw material components II, and collaboration promotes beneficial bacteria of intestinal tract to increase
Grow and account for the 0.15% of feed gross weight with the weight of the additive of field planting；Raw material components I with II successively feedstuff mix, first
Raw material components I are added in feedstuff, after stirring 1-2 minutes, input raw material components II continue to stir 1-2 minutes.
The specific feature of this programme also has, 20-30 parts of raw material components I, 70-80 parts of raw material components II, wherein oligomeric wood
It is sugared 10-15 parts, clostridium butyricum 10-15 parts, 70-80 parts of fulvic acid sodium humate high.
The present invention has following good effect：
1st, xylo-oligosaccharide promotes clostridium butyricum propagation, without being metabolized by livestock and poultry and harmful bacteria, selectively promotes clostridium butyricum in intestines
The propagation in road.
2nd, clostridium butyricum gemma state can tolerate the extreme environments such as high temperature, drying, therefore in feed high temperature granulating process
In ensure that clostridium butyricum is not damaged, it is ensured that clostridium butyricum enters animal and bird intestines and plays prebiotic activity with viable bacteria state.
3rd, with xylo-oligosaccharide as carbon source in clostridium butyricum fermentation medium, clostridium butyricum is made to open xylo-oligosaccharide metabolism logical
Road, is easy to bacterial strain quickly to start growth and breeding with xylo-oligosaccharide as carbon source after entering enteron aisle, shortens growth of microorganism
Required lag phase.
4th, using Phanerochaete chrysosporium bioremediation, fulvic acid in sodium humate is improved to 12- by≤1%
16%, the method reaction is gentle, and course of reaction will not generate pollutant.
5th, fulvic acid promotes the differentiation of capillary and regeneration in damaged part tissue, is conveyed by strengthening to wounded tissue
Nutriment, promotes the regeneration of wounded tissue, it is ensured that the complete and development of gut epithelium tissue, and then improves epithelial mucosal surface
Probiotics adhesion receptors level, promotes probiotics in the field planting of enteron aisle.
Invention formulation improves preparation by xylo-oligosaccharide, clostridium butyricum and fulvic acid sodium humate synergy high
Effect in terms of intestinal microecology balance, enhancing intestinal health is maintained, enhances its effect on livestock and poultry cultivation, makes product
With stronger competitiveness.
Phanerochaete chrysosporium is purchased from Chinese industrial Microbiological Culture Collection administrative center, address：Make Fengtai District, Beijing City
Building 15, first street 110.Classification And Nomenclature：Phanerochaete chrysosporium；Latin name：Phanerochaete chrysosporium；
Deposit number CICC 40299.
Clostridium butyricum is purchased from Chinese industrial Microbiological Culture Collection administrative center, address：Zaojia Street, Fengtai District, Beijing City 110
Number Building 15.Classification And Nomenclature：Clostridium butyricum；Latin name：Clostridium butyricum Deposit number CICC 20036.
Brief description of the drawings
Fig. 1 is xylo-oligosaccharide of the present invention, clostridium butyricum and fulvic acid sodium humate Synergistic Mechanisms schematic diagram high.
Specific embodiment
Embodiment 1：It is a kind of cooperate with promote proliferation of intestinal probiotics with field planting additive, it be by it is following in parts by weight
Raw material be made：Xylo-oligosaccharide 10-15 parts, clostridium butyricum 10-15 parts, 70-80 parts of fulvic acid sodium humate high.The oligomeric wood
Sugar refers to the wherein degree of polymerization for the xylo-oligosaccharide content of 2-4 is more than 55%（Product is by the Shandong dragon power limited public affairs of biotechnology share
Department's production）.Fulvic acid content >=12% in the fulvic acid sodium humate high.Described clostridium butyricum active bacteria number is 1010-1011
Bacterium colony/gram.
It is prepared by fulvic acid sodium humate high：
（1）The preparation of mycelia liquid：By Phanerochaete chrysosporium（Purchased from Chinese industrial Microbiological Culture Collection administrative center, numbering
CICC 40299,Phanerochaete chrysosporium）It is inoculated into malt juice liquid medium, in 39 degrees Celsius of trainings
Mycelia liquid is prepared after supporting 4 days.
（2）By mycelia liquid, pure water and sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces, fulvic acid content
It is 0.4%）According to weight 1：1：1 mixing, inorganic salts are added by mixture weight（Urea 0.1%, dipotassium hydrogen phosphate 0.1%, phosphoric acid
Potassium dihydrogen 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%）.
（3）PH is controlled for 4.8, temperature is 30 degrees Celsius, 60 revs/min of stirring reactions 10 days.
（4）Reaction solution is used into roller drying, sheet products are obtained, fulvic acid content is 12.6%.
Clostridium butyricum is to be made by the steps to form：
（1）Butyrate spindle bacillus seed nutrient solution is made, wherein bacterial concentration is 5.0 × 108CFU/ml；
（2）Culture medium is made, specially：High temperature beancake powder 1.8%, urea 0.1%, corn pulp 0.1%, xylo-oligosaccharide 3.0%, phosphoric acid
Hydrogen dipotassium 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%；
（3）Butyrate spindle bacillus seed liquid is added in culture medium in 2% ratio using peristaltic pump, 35 DEG C of quiescent cultures 28 hours；
With xylo-oligosaccharide as sole carbon source in clostridium butyricum fermentation process；Sampling detection total viable count, total viable count be 2.7 ×
109CFU/ml, gemma rate is 100%；
（4）Zymotic fluid is centrifuged using tube centrifuge, carrier corncob, the aeration-drying below 40 DEG C, moisture control is added
5%, 100 mesh steel sieve was crushed, obtained clostridium butyricum.
After detection is qualified, packaging and storage.
Embodiment 2：It is a kind of cooperate with promote proliferation of intestinal probiotics with field planting additive, it be by it is following in parts by weight
Raw material be made：Xylo-oligosaccharide 10-15 parts, clostridium butyricum 10-15 parts, 70-80 parts of fulvic acid sodium humate high.The oligomeric wood
Sugar refers to the wherein degree of polymerization for the xylo-oligosaccharide content of 2-4 is more than 55%（Product is by the Shandong dragon power limited public affairs of biotechnology share
Department's production）.Fulvic acid content >=12% in the fulvic acid sodium humate high.It is prepared by fulvic acid sodium humate high：
（1）The preparation of mycelia liquid：By Phanerochaete chrysosporium（Purchased from Chinese industrial Microbiological Culture Collection administrative center, numbering
CICC 40299,Phanerochaete chrysosporium）It is inoculated into malt juice liquid medium, in 39 degrees Celsius of trainings
Mycelia liquid is prepared after supporting 6 days.
（2）By mycelia liquid, pure water and sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces, fulvic acid content
It is 0.4%）According to weight 2：1.5：3 mixing, inorganic salts are added by mixture weight（Urea 0.2%, dipotassium hydrogen phosphate 0.3%, phosphorus
Acid dihydride potassium 0.4%, magnesium sulfate 0.2%, manganese sulfate 0.03%）.
（3）PH is controlled for 5.0, temperature is 34 degrees Celsius, 60 revs/min of stirring reactions 6 days.
（4）Reaction solution is used into roller drying, sheet products are obtained, fulvic acid content is 16.8%.
Described clostridium butyricum active bacteria number is 1010-1011Bacterium colony/gram.Clostridium butyricum is purchased from Chinese industrial microorganism fungus kind
Preservation administrative center, numbering CICC 20036,Clostridium butyricum.Clostridium butyricum is to be made by the steps
Form：
（1）Butyrate spindle bacillus seed nutrient solution is made, wherein bacterial concentration is 5.0 × 108CFU/ml；
（2）Culture medium is made, specially：High temperature beancake powder 1.8%, urea 0.1%, corn pulp 0.1%, xylo-oligosaccharide 3.0%, phosphoric acid
Hydrogen dipotassium 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%；
（3）Butyrate spindle bacillus seed liquid is added in culture medium in 2% ratio using peristaltic pump, 35 DEG C of quiescent cultures 28 hours；
With xylo-oligosaccharide as sole carbon source in clostridium butyricum fermentation process；Sampling detection total viable count, total viable count be 2.7 ×
109CFU/ml, gemma rate is 100%；
（4）Zymotic fluid is centrifuged using tube centrifuge, carrier corncob, the aeration-drying below 40 DEG C, moisture control is added
5%, 100 mesh steel sieve was crushed, obtained clostridium butyricum.
After detection is qualified, packaging and storage.
Embodiment 3：The present embodiment part same as Example 2 is repeated no more, and difference is：Fulvic acid humic acid high
It is prepared by sodium
（1）The preparation of mycelia liquid：Phanerochaete chrysosporium is inoculated into malt juice liquid medium, 8 are cultivated in 39 degrees Celsius
Mycelia liquid is prepared after it.
（2）By mycelia liquid, pure water and sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces）According to weight 3：
2：4 mixing, inorganic salts are added by mixture weight（Urea 0.3%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate
0.3%th, manganese sulfate 0.05%）.
（3）PH is controlled for 6.0, temperature is 37 degrees Celsius, 60 revs/min of stirring reactions 8 days.
（4）Reaction solution is used into roller drying, sheet products are obtained, fulvic acid content is 14.4%.
Additive Product growing and fattening pigs clinical practice embodiment of the present invention：
The 5-7 months in 2016 carry out feeding experiment on Ji'nan Haihua Bio-Technology Co., Ltd.'s experiment pig farm, in the green peace food in Jinan
Co., Ltd carries out butchering split-plot experiment.Selection kind is identical, agematched 60kg body weight DLY market pig 800
Head, is divided into 16 groups, every group 50.Corresponding invention formulation, control group fed Linyi and Kang Yuan feeds are grouped and added by form 1
The mixed feed of Co., Ltd.Feeding experiment to average weight is that 100kg or so terminates, body weight, feed intake to growing and fattening pigs and
Feedstuff-meat ratio etc. is calculated.
Embodiment 4：
Preparation A：I 20 parts of raw material components, II 80 parts of raw material components, 10 parts of xylo-oligosaccharide in final preparation product, clostridium butyricum 10
Part, fulvic acid sodium humate high（Rule of origin is in embodiment 2）80 parts.Addition is 0.15 % in fattening pannage, specific raw
Long pointer parameter is shown in Table 1.
Table 1
Embodiment 5：
A kind of to cooperate with the use for promoting proliferation of intestinal probiotics and the additive of field planting, xylo-oligosaccharide is mixed with clostridium butyricum
Raw material components I；Fulvic acid sodium humate high is raw material components II, and collaboration promotes proliferation of intestinal probiotics with the additive of field planting
Weight accounts for the 0.15% of feed gross weight；Raw material components I with II successively feedstuff mix, raw material components I are added to feeding first
In material raw material, after stirring 1-2 minutes, input raw material components II continue to stir 1-2 minutes.
Preparation B：I 25 parts of raw material components, II 75 parts of raw material components, in final preparation product
12.5 parts of xylo-oligosaccharide, 12.5 parts of clostridium butyricum, fulvic acid sodium humate high（Rule of origin is in embodiment 2）75 parts.Educate
The specific growth indexes parameter of big porker is shown in Table 2.
Table 2
Embodiment 6：A kind of collaboration promotes the application method of proliferation of intestinal probiotics and the additive of field planting, the present embodiment and implementation
The something in common of example 4 is repeated no more, and difference is：
Formulation C：I 30 parts of raw material components, II 70 parts of raw material components, 15 parts of xylo-oligosaccharide in final preparation product, clostridium butyricum 15
Part, fulvic acid sodium humate high（Rule of origin is in embodiment 2）70 parts.The specific growth indexes parameter of growing and fattening pigs is shown in Table 3.
Table 3
By embodiment 4-6（Table 1-3）, by adding inventive article, experiment pig daily gain, drop can be significantly improved
Low feedstuff-meat ratio and diarrhea rate, improve food utilization efficiency, and then improve culture benefit.
Embodiment 7：It is a kind of cooperate with promote proliferation of intestinal probiotics with field planting additive application method, the present embodiment with
The something in common of embodiment 4 is repeated no more, and difference is：
Preparation D：I 25 parts of raw material components, II 75 parts of raw material components, in final preparation product
12.5 parts of xylo-oligosaccharide, 12.5 parts of clostridium butyricum, sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces, yellow rotten
Acid content is 0.4%, is that embodiment 1-3 technologies are raw materials used, without embodiment 1-3 technical finesses）75 parts.In fattening pannage
Addition is 0.15%, and specific growth indexes parameter is shown in Table 4.
Table 4
And then from embodiment 7, fulvic acid content plays an important roll for product effect in sodium humate, embodiment 7 and 5
In it is identical in other compositions, only in the case of fulvic acid changes of contents, the daily gain of growing and fattening pigs, feedstuff-meat ratio and diarrhea rate level
Show inferior position.
Embodiment 8（Comparative example）
Preparation E:I 25 parts of raw material components, II 75 parts of raw material components, 12.5 parts of xylo-oligosaccharide in final preparation product, butyric acid shuttle
12.5 parts of bacterium, fulvic acid sodium humate high（Rule of origin is in embodiment 2）75 parts, three mixes first, then with feedstuff
Mix.Addition is 0.15 % in fattening pannage, and specific growth indexes parameter is shown in Table 5.
Embodiment 8 compared with Example 5 compared with, xylo-oligosaccharide, clostridium butyricum and fulvic acid sodium humate high are directly mixed,
Although the final content of three is identical, effect is but far away from being divided into adding for raw material components I and raw material components II in embodiment 5
Add mode, further illustrates the importance of product substep adding method.
Embodiment 9：A kind of to cooperate with the preparation method for promoting proliferation of intestinal probiotics and the additive of field planting, collaboration promotes intestines
Road proliferation of probiotics is made up with the additive of field planting of following raw materials in parts by weight：Xylo-oligosaccharide 10-15 parts, butyric acid shuttle
Bacterium 10-15 parts, 70-80 parts of fulvic acid sodium humate high.The xylo-oligosaccharide refers to the wherein degree of polymerization for the xylo-oligosaccharide of 2-4 contains
Measure is more than 55%（Product is produced by Shandong Longli Biology Science and Technology Co., Ltd）.
Fulvic acid content >=12% in the fulvic acid sodium humate high.It is prepared by fulvic acid sodium humate high：
（1）The preparation of mycelia liquid：Phanerochaete chrysosporium is inoculated into malt juice liquid medium, 6 are cultivated in 39 degrees Celsius
Mycelia liquid is prepared after it.Phanerochaete chrysosporium is purchased from Chinese industrial Microbiological Culture Collection administrative center, numbering CICC
40299,Phanerochaete chrysosporium。
（2）By mycelia liquid, pure water and sodium humate（Ji'nan Haihua Bio-Technology Co., Ltd. produces, fulvic acid content
It is 0.4%）According to weight 2：1.5：3 mixing, inorganic salts are added by mixture weight（Urea 0.2%, dipotassium hydrogen phosphate 0.3%, phosphorus
Acid dihydride potassium 0.4%, magnesium sulfate 0.2%, manganese sulfate 0.03%）.
（3）PH is controlled for 5.0, temperature is 34 degrees Celsius, 60 revs/min of stirring reactions 6 days.
（4）Reaction solution is used into roller drying, sheet products are obtained, fulvic acid content is 16.8%.
Described clostridium butyricum active bacteria number is 1010-1011Bacterium colony/gram.Clostridium butyricum is purchased from Chinese industrial microorganism fungus kind
Preservation administrative center, numbering CICC 20036,Clostridium butyricum.Clostridium butyricum is to be made by the steps
Form：
（1）Butyrate spindle bacillus seed nutrient solution is made, wherein bacterial concentration is 5.0 × 108CFU/ml；
（2）Culture medium is made, specially：High temperature beancake powder 1.8%, urea 0.1%, corn pulp 0.1%, xylo-oligosaccharide 3.0%, phosphoric acid
Hydrogen dipotassium 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%；
（3）Butyrate spindle bacillus seed liquid is added in culture medium in 2% ratio using peristaltic pump, 35 DEG C of quiescent cultures 28 hours；
With xylo-oligosaccharide as sole carbon source in clostridium butyricum fermentation process；Sampling detection total viable count, total viable count be 2.7 ×
109CFU/ml, gemma rate is 100%；
（4）Zymotic fluid is centrifuged using tube centrifuge, carrier corncob, the aeration-drying below 40 DEG C, moisture control is added
5%, 100 mesh steel sieve was crushed, obtained clostridium butyricum.
After detection is qualified, packaging and storage.
Claims (7)
1. it is a kind of cooperate with promote proliferation of intestinal probiotics with field planting additive, it is characterized in that, it be by it is following in parts by weight
Raw material be made：Xylo-oligosaccharide 10-15 parts, clostridium butyricum 10-15 parts, 70-80 parts of fulvic acid sodium humate high.
2. a kind of collaboration as claimed in claim 1 promotes the application method of proliferation of intestinal probiotics and the additive of field planting, its
It is characterized in that xylo-oligosaccharide is mixed and made into raw material components I with clostridium butyricum；Fulvic acid sodium humate high is raw material components II, collaboration
Proliferation of intestinal probiotics is promoted to account for the 0.15% of feed gross weight with the weight of the additive of field planting；Raw material components I and II successively with
Feed mixes, and raw material components I are added in feedstuff first, and after stirring 1-2 minutes, input raw material components II continue to stir
1-2 minutes.
3. collaboration according to claim 1 promotes the additive of proliferation of intestinal probiotics and field planting, it is characterized in that, it is described
Xylo-oligosaccharide refers to the wherein degree of polymerization for the xylo-oligosaccharide content of 2-4 is more than 55%.
4. collaboration according to claim 1 promotes the additive of proliferation of intestinal probiotics and field planting, it is characterized in that, the height
Fulvic acid content >=12% in fulvic acid sodium humate.
5. collaboration according to claim 4 promotes the additive of proliferation of intestinal probiotics and field planting, it is characterized in that high yellow rotten
Sour sodium phytate is to be made by the steps to form：
（1）Phanerochaete chrysosporium is inoculated into malt juice liquid medium, mycelia is prepared after being cultivated 4-8 days in 39 degrees Celsius
Liquid；
（2）By mycelia liquid, pure water and sodium humate according to weight 1-3：1-2：1-4 mixes, by total weight of the mixture after mixing
For radix addition inorganic salts are specifically included：Urea 0.1-0.3%, dipotassium hydrogen phosphate 0.1-0.5%, potassium dihydrogen phosphate 0.1-0.5%,
Magnesium sulfate 0.1-0.3%, manganese sulfate 0.01-0.05%；
（3）PH is controlled for 4.8-6.0, temperature is 30-37 degrees Celsius, 60 revs/min of stirring reactions 5-10 days；
（4）By step（3）The reaction solution of acquisition uses roller drying, obtains fulvic acid sodium humate sheet products high.
6. collaboration according to claim 1 promotes the additive of proliferation of intestinal probiotics and field planting, it is characterized in that, it is described
Clostridium butyricum active bacteria number is 1010-1011Bacterium colony/gram.
7. collaboration according to claim 6 promotes the additive of proliferation of intestinal probiotics and field planting, it is characterized in that butyric acid shuttle
Bacterium is to be made by the steps to form：（1）Make butyrate spindle bacillus seed nutrient solution, wherein bacterial concentration be 5.0 ×
108CFU/ml；
（2）Culture medium is made, specially：High temperature beancake powder 1.8%, urea 0.1%, corn pulp 0.1%, xylo-oligosaccharide 3.0%, phosphoric acid
Hydrogen dipotassium 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, manganese sulfate 0.01%；
（3）Butyrate spindle bacillus seed liquid is added in culture medium in 2% ratio using peristaltic pump, 35 DEG C of quiescent cultures 28 hours；
With xylo-oligosaccharide as sole carbon source in clostridium butyricum fermentation process；Sampling detection total viable count, total viable count be 2.7 ×
109CFU/ml, gemma rate is 100%；
（4）Zymotic fluid is centrifuged using tube centrifuge, carrier corncob, the aeration-drying below 40 DEG C, moisture control is added
5%, 100 mesh steel sieve was crushed, obtained clostridium butyricum.
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