CN103451132A - Composite microecological preparation used for fermentation of breeding feeds - Google Patents
Composite microecological preparation used for fermentation of breeding feeds Download PDFInfo
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Abstract
The invention provides a composite microecological preparation used for fermentation of breeding feeds. A preparation method of the composite microecological preparation comprises the following steps: respectively activating lactobacillus acidophilus, bacillus subtilis, rhodopseudomonas palustris and saccharomyces cerevisiae in a freezing preservation tube in a slant, and then purifying to obtain various slant strains for later use; utilizing various slant strains to prepare corresponding original strain solutions; inoculating the original strain solutions into a sterilized fermentation medium I to be cultured according to a strain quantity ratio of 1:1:1:1 to obtain first-class seeds of the composite microecological preparation; inoculating the first-class seeds into a sterilized fermentation medium II to be cultured according to an inoculum size of 5% to obtain second-class seeds of the composite microecological preparation; finally inoculating the second-class seeds into a sterilized fermentation medium III to be cultured according to an inoculum size of 5% to obtain a finished product of the composite microecological preparation. The feeds fermented by the composite microecological preparation can promote growth of pigs, increase daily gains of pigs and reduce the feed conversion ratio when utilized to feed pigs, thus increasing the economic benefits.
Description
[technical field]
The present invention relates to a kind of compound micro-ecological preparation that field is used that cultivates, relate in particular to a kind of compound micro-ecological preparation for the feed ingredient fermentative processing.
[background technology]
Lactobacterium acidophilum belongs to lactobacillus, Gram-positive bacillus, and the end of bar is rounded, mainly exists in small intestine, and release lactic acid, acetic acid and the antibiotic that some work to harmful bacteria, adjust the intestinal microflora balance, suppresses the propagation of enteron aisle undesirable microorganism.
Rhodopseudomonas palustris belongs to a kind of of photosynthetic bacterium (english abbreviation PSB), is one of microorganism the most ancient on the earth.The PSB thalline is nutritious, protein content is up to 65%, and is rich in various bioactivators, and has strong adaptability, can tolerate the organic waste water of high density and stronger decomposition and inversion ability, the poisonous substances such as phenol, cyanogen also be had to the characteristics such as certain tolerance and capacity of decomposition; It can be used as feed level microbe additive.
Yeast saccharomyces cerevisiae, claim again bread yeast or budding yeast; Be used as the eucaryon model animals in modern molecule and cytobiology, its effect is equivalent to the model animals intestinal bacteria of protokaryon.Yeast saccharomyces cerevisiae is the most frequently used biological species in fermentation.The cell of yeast saccharomyces cerevisiae is spherical or avette, diameter 5-10 μ m.
Subtilis is a kind of of bacillus; 0.7～0.8 * 2～3 microns of individual cells, uniform coloring; Without pod membrane, peritrichous, can move; Gram-positive microorganism, 0.6～0.9 * 1.0～1.5 microns of gemma, oval to column, be positioned at thalline central authorities or slightly inclined to one side, after sporulation, thalline does not expand; The bacterium colony surface irregularity is opaque, dirty white or micro-yellow, and while growing in liquid medium within, the normal wrinkle mould that forms; Aerophil; Its available protein, multiple sugar and starch, decompose tryptophane and form indoles.It has following effect: the subtilyne produced in subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, and these active substances have obvious restraining effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Subtilis consumes rapidly the free oxygen in environment, causes the enteron aisle hypoxemia, promotes the growth of useful anerobe, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacterium growth; Subtilis thalline self synthesizes the enzymes such as α-amylase, proteolytic enzyme, lipase, cellulase, in digestive tube with animal body in digestive enzymes together play a role; The multiple vitamin B group such as energy synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising animal body internal interference element and scavenger cell.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound micro-ecological preparation for the feed ingredient fermentative processing.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound micro-ecological preparation for the feed ingredient fermentative processing, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in the freezing pipe, subtilis, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Lactobacterium acidophilum slant strains, subtilis slant strains, Rhodopseudomonas palustris slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 37 ℃ of lower anaerobism cultivations and obtains the Lactobacterium acidophilum original bacteria liquid in 1-2 days; The subtilis slant strains is inoculated in sterilized liquid nutrient medium II, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains the Rhodopseudomonas palustris original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Lactobacterium acidophilum original bacteria liquid, subtilis original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 1:1:1:1, particularly: first inoculate subtilis original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, cultivate 3 days in 37 ℃ of lower anaerobism, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound micro-ecological preparation.
Further, the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium I, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.
Further, the concrete operations that in described step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on the slant medium II, then aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 20min; And the component of this slant medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.
Further, the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium III, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of liquid nutrient medium I in described step (2): peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
Further, the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
The beneficial effect of a kind of compound micro-ecological preparation for the feed ingredient fermentative processing of the present invention is: this compound micro-ecological preparation can produce multiple digestive ferment in the live pig body, they in digestive process with the live pig body in enzyme play synergy, the protein, fat and the complicated carbohydrate that are conducive to degrade in feed, thereby improve the transformation efficiency of feed, promote pig growth, improve the day weight gain of live pig.
[embodiment]
A kind of compound micro-ecological preparation for the feed ingredient fermentative processing, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in the freezing pipe, subtilis, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Lactobacterium acidophilum slant strains, subtilis slant strains, Rhodopseudomonas palustris slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 37 ℃ of lower anaerobism cultivations and obtains the Lactobacterium acidophilum original bacteria liquid in 1-2 days; The subtilis slant strains is inoculated in sterilized liquid nutrient medium II, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains the Rhodopseudomonas palustris original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Lactobacterium acidophilum original bacteria liquid, subtilis original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 1:1:1:1, particularly: first inoculate subtilis original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, cultivate 3 days in 37 ℃ of lower anaerobism, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium I, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.The concrete operations that in step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on the slant medium II, then aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 20min; And the component of this slant medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.The concrete operations that in step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium III, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.The concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III in step (5): tangerine water 5%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
It is the fermentative processing that is applied to pannage that the present invention prepares the gained compound micro-ecological preparation, particularly: the present invention who gets 1 weight part prepares the gained compound micro-ecological preparation and adds 12 weight part clear water to be diluted, after dilution, with the feed I of 25 parts of weight, fully stir, form the mix feed of half dry and wet state, to after this mix feed dress plastic bag sealing, pile up stack retting afterwards, color is deeply brown, form is soft and give out the vinic acid flavor until the feed in bag becomes to carry out normal temperature fermentation, obtains fermented feed.Wherein, the feed I can be selected existing commodity perfect compound feed on market, also can select pig farm autogamy feed.
In addition, the applicant, for the effect of compound micro-ecological preparation of the present invention is described, will utilize the fermented feed obtained after compound micro-ecological preparation fermentative processing of the present invention to be fed the live pig test, particularly:
On the Fujian Longyan pig farm, select that age in days is identical, totally 40 of big or small basically identical, normal, the health pig of body weight more than 40 kilograms of growing of body weight, be divided at random test group and control group, 20 every group.In test group, fermented feed is mixed in pig farm autogamy feed, the live pig of feeding after fully mixing thoroughly, and the per-cent that adds fermented feed to account for the feed gross weight is after initial 5%, 5 day after 10%, 10 day 20%; The control group pig farm autogamy feed of only feeding; The pig of test pig and control group is raised at same colony house subfield, unified management, and envrionment conditions is identical, and all carries out immunization, endoparasite and ectoparasite before test and drive away, and carries out the adaptability raising through 7 day preliminary trial period, enters afterwards experimental stage; It is principle that the feed standard be take in groove not fracture, days reinforced 2 times, and free choice feeding, constantly, test period is 75 days to clean drinking-water.When experiment starts and finishes, each first-born pig is weighed separately, experimental result is: the average daily gain of test group live pig is 0.78 kilogram, and the control group average daily gain is 0.71 kilogram, and test group is compared control group day weight gain raising 9.9%; The material anharmonic ratio 3.14:1 of test group, the material anharmonic ratio 3.44:1 of control group, test group is saved 0.22 kilogram, feed than one kilogram of gross weight of the every increase of control group, and test group has reduced by 7% than control group feed consumption.Therefore, from test-results, adopt and added the forage feed live pig that utilizes the fermented feed obtained after compound micro-ecological preparation fermentative processing of the present invention, can improve the day weight gain of live pig, reduce feedstuff-meat ratio, increase economic efficiency.
In addition, by basestocks and Preblend, the weight ratio by 25:1 mixes the pig farm autogamy feed described in the present invention, and wherein basestocks is comprised of the component of following weight part: 65 parts of corns, 25 parts of dregs of beans, 5 parts, wheat bran, 5 parts, rice bran.
To sum up, this compound micro-ecological preparation can produce multiple digestive ferment in the live pig body, they in digestive process with the live pig body in enzyme play synergy, the protein, fat and the complicated carbohydrate that are conducive to degrade in feed, thereby improve the transformation efficiency of feed, promote pig growth, improve the day weight gain of live pig.
Claims (9)
1. the compound micro-ecological preparation for the feed ingredient fermentative processing, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in the freezing pipe, subtilis, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain Lactobacterium acidophilum slant strains, subtilis slant strains, Rhodopseudomonas palustris slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 37 ℃ of lower anaerobism cultivations and obtains the Lactobacterium acidophilum original bacteria liquid in 1-2 days; The subtilis slant strains is inoculated in sterilized liquid nutrient medium II, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains Rhodopseudomonas palustris inclined-plane bacterium original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium IV, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by Lactobacterium acidophilum original bacteria liquid, subtilis original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid, and the yeast saccharomyces cerevisiae original bacteria liquid be inoculated in sterilized fermention medium I and cultivated by the bacterium amount ratio of 1:1:1:1, particularly: first inoculate subtilis original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, cultivate 3 days in 37 ℃ of lower anaerobism, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound micro-ecological preparation.
2. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium I, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.
3. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the concrete operations that in described step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on the slant medium II, then aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 20min; And the component of this slant medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.
4. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium III, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.
5. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium IV to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
6. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, is characterized in that: the component of liquid nutrient medium I in described step (2): peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium II: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
7. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
8. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
9. a kind of compound micro-ecological preparation for the feed ingredient fermentative processing according to claim 1, it is characterized in that: the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
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| CN105661091A (en) * | 2016-01-29 | 2016-06-15 | 刘健 | Preparation method of antibiotic-free fattening chicken feed |
| CN108208325A (en) * | 2018-01-19 | 2018-06-29 | 深圳市帝之道科技贸易有限公司 | A kind of probiotics composite mineral matter additive |
| CN109055279A (en) * | 2018-09-25 | 2018-12-21 | 厦门惠盈动物科技有限公司 | A kind of composite bacillus preparation and its preparation method and application |
| CN112624350A (en) * | 2020-12-25 | 2021-04-09 | 自然资源部第三海洋研究所 | Microecological preparation for freshwater aquaculture |
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