CN103355472A - Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof - Google Patents
Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial fermentation agent for cottonseed meal detoxification and a preparation method thereof. According to the preparation method, saccharomyces cerevisiae and bacillus subtilis serve as active strains and are subjected to expanding culture by using liquid fermentation, a carrier is added, and then, the microbial fermentation agent is prepared. According to the microbial fermentation agent, free gossypol in cottonseed meal can be degraded effectively, crude fibers are degraded and softened, the palatability of the cottonseed meal is improved, a large number of nutrition and hormone substances, such as active probiotics, bioactive small-peptide amino acids, various biochemical enzymes, somatomedins and the like, are secreted and synthesized, and the immunity of animals is effectively improved by microbial thalli protein, beta-glucan and the like in the microbial fermentation agent. The microbial fermentation agent further has the advantages that the strains and fermentation processes are simple, the cost is low, the consumption of the strains in cottonseed meal fermentation is low, the cost for the cottonseed meal fermentation is low, and the like.
Description
Technical field
The present invention relates to a kind of leavening that can be used for producing the animal feed raw material, relate in particular to a kind of leavening, its preparation method that comprises saccharomyces cerevisiae, bacillus subtilis, and utilize described leavening to process the method for cotton dregs detoxification.
Background technology
China is populous, grain and production of fodder are the weak links of national economy always, because the increase of population growth, plough minimizing and meat product consumption figure, Grain Supply-demand Balance is very fragile, the per-capita share of grain once at 400kg always, wherein, 40% of total output of grain is used for production of fodder, and therefore developing high-efficiency feed industry, improving grain is to satisfy people to the optimal path of the increasing demand of meat, fowl, fish, egg to the transformation efficiency of livestock products and efficiency of feed utilization, development of new feedstuff.But China's protein feed resource is seriously deficient at present, and will become for a long time the bottleneck of China's animal husbandry development, and feed resource shortage situation is constantly aggravated, and the feed disparities between supply and demand are outstanding.
In the cotton growing area, the byproducts such as cotton dregs and cotton seed hulls become the main source that replenishes of feed breach.Cotton dregs and cottonseed protein all are the high-quality protein raw materials, and crude protein content is higher, and in the 36-41% scope, total amino acid content is 83-87% in the thick protein; Crude fat content is high, is vitamin and linoleic good source, and content of mineral substances and soybean cake (dregs of rice) are close, can improve breeding performonce fo animals, reduces feed cost.There is the cottonseed more than 1,000 ten thousand tons every year in China, produces Cottonseed Meal per year and reaches more than 6,000,000 tons, and stock number accounts for the whole world first.
But, because the cottonseed cake cotton dregs contain noxious material and the ANFs existence such as free gossypol and cyclopropylene acid, to the toxic effect of livestock and poultry; In addition, because liquefaction adds and do not remove cot man-hour, cause in cottonseed cake, the cotton dregs crude fiber content higher, palatability is poor etc., and its utilization is very limited.
Traditional poison-removing method comprises organic extraction, ferrous sulfate processing, calcium hydroxide processing etc., wherein, the organic solvent leach extraction method causes organic solvent residual, and livestock and poultry are had potential harm, ferrous sulfate is processed and can be reduced feed quality, and it is low that calcium hydroxide is processed detoxification efficient.
The method of microbial fermentation is a kind of method with development prospect, microbial fermentation not only can remove the gossypol in the cotton cake dregs, but also can provide the protein content of cotton cake dregs, retaining in the fermentation substrate permitted plurality of enzymes, vitamin, amino acid, and somatomedin, therefore utilize microbial fermentation technology, the development of new feed resource, producing protein feeds and new additive agent more and more comes into one's own, particularly enter after 21 century, utilize the forage protein of micro-organisms, enzyme preparation, amino acid, vitamin, the use of the feed products such as antibiotic and probiotic micro-organisms preparation makes fermentation engineering obtain using widely in feed industry.Under efficient factoring effect, crude fibre etc. can not be changed into by the polymeric carbohydrate that livestock and poultry absorb the low molecular compound that can doubly absorb; The multiple beneficial flora can absorb organic nitrogen, the inorganic nitrogen that can not be absorbed by livestock and poultry or utilize in addition, and makes it be converted into the higher bacterium protein of nutritional labeling; And profitable strain can increase immunity of livestock, the generation of minimizing disease.
Patent CN102823726A discloses a kind of biofermentation technique that improves cottonseed meal content and detoxification, use candida utili, aspergillus niger, bacillus subtilis and lactobacillus acidophilus zymotic fluid are as bacterial classification, can effectively degrade free gossypol in the cotton dregs, improve the protein quality of cotton dregs, improved the mouthfeel of cotton dregs.
Patent CN102805208A discloses a kind of preparation method of high-lysine reduce toxicity cotton dregs, use Candida tropicalis, candida utili bacterium, saccharomyces cerevisiae and lactobacillus ferment liquid as bacterial classification, Candida tropicalis and lactobacillus can effectively be cotton cake toxicity removal, candida utili bacterium and saccharomyces cerevisiae can improve the content of lysine, to solve the problem of cotton dregs lysine content deficiency.
From the prior art, the fermentation of cottonseed cake, cotton dregs generally all is to adopt multiple bacterial classification composite fermentation, and the use of multiple bacterial classification requires for fermentation condition can be very strict, and cost is high, is unfavorable for the industrialization use.
Summary of the invention
For the problems referred to above, the invention provides a kind of cotton dregs Free Gossypol of effectively degrading, improve its palatability, degraded and softening crude fibre, secrete and synthetic a large amount of active probiotics a kind of microbe leaven of improving food conversion ratio simultaneously and its preparation method and application.
First aspect of the present invention provides a kind of cotton cake toxicity removal microbe leaven, and the active bacterium of described microbe leaven comprises saccharomyces cerevisiae and bacillus subtilis.
Wherein, saccharomyces cerevisiae and bacillus subtilis part by weight are preferably (80-90): (10-20).
The first preferred embodiment of first aspect according to the present invention, wherein, the active bacterium of described microbe leaven is comprised of saccharomyces cerevisiae and bacillus subtilis; Wherein, saccharomyces cerevisiae and bacillus subtilis part by weight are preferably (80-90): (10-20).
In the above-mentioned cotton cake toxicity removal microbe leaven of the present invention, can also comprise carrier, described carrier can be solid carrier and/or liquid-carrier.
Contain in the cotton cake toxicity removal microbe leaven of carrier, total viable bacteria content is preferably 1 * 10
8-1 * 10
10Cfu/g, more preferably 5 * 10
8-5 * 10
9Cfu/g, more preferably 1 * 10
9-3 * 10
9Cfu/g is such as 2 * 10
9Cfu/g.
Wherein, described carrier is preferably solid carrier, and more preferably starch most preferably is cornstarch.
The second preferred embodiment of first aspect according to the present invention, described carrier is solid carrier, is preferably starch, such as cornstarch, in the described cotton cake toxicity removal microbe leaven, water content≤15%, be preferably≤12%.
Second aspect of the present invention provides a kind of method for preparing such as above-mentioned cotton cake toxicity removal microbe leaven, and step comprises:
Step 1 will comprise that the active bacterium of saccharomyces cerevisiae, bacillus subtilis mixes, cultivate,
Step 2 is mixed culture with carrier, obtain described microbe leaven.
Wherein, saccharomyces cerevisiae and bacillus subtilis part by weight are preferably (80-90): (10-20).
Wherein, described active bacterium is preferably by saccharomyces cerevisiae, bacillus subtilis and forms.
Wherein, described carrier can be liquid-carrier and/or solid carrier, solid carrier more preferably, and more preferably starch most preferably is cornstarch.
In the step 1, described cultivation is preferably and comprises that liquid at high speed is cultivated and solid afterwards enlarges cultivation.
Wherein, the method that described liquid at high speed is cultivated is: the active bacterium that will mix is inoculated in the fluid nutrient medium, to total viable count 〉=100,000,000/ml concentration, be preferably 〉=5-50 hundred million/ml concentration, more preferably 〉=10-30 hundred million/ml concentration, more preferably 2,000,000,000/ml concentration, and pH value stops fermentation when reaching 6.5-7.5.
Described culture medium can be sterilized before use, is preferably high-temperature sterilization, and the temperature of described high-temperature sterilization is preferably 120-150 ° of C, and the time is preferably 15-20min.
During described liquid at high speed was cultivated, the active bacterium that preferably will mix was according to weight ratio 1: (20-25) be inoculated in the fluid nutrient medium.
During described liquid at high speed was cultivated, cultivation temperature was preferably 25-38 ° of C, and 28-35 ° of C more preferably is such as 30 ° of C, 33 ° of C etc.
During described liquid at high speed was cultivated, incubation time was preferably 20-40 hour, and more preferably 24-36 hour, such as 25 hours, 30 hours, 35 hours etc.
Described liquid at high speed is cultivated under shaking table cultivation and/or stirring condition and is carried out, and wherein, the rotating speed that shaking table is cultivated is preferably 200-220r/min.
A kind of preferred embodiment of second aspect according to the present invention, described carrier is solid carrier, is preferably starch, such as cornstarch.
In the step 2, the part by weight of culture and carrier is preferably (0.1-1): 1, more preferably (0.2-0.7): 1, more preferably (0.3-5): 1, such as 0.4: 1.
In the preferred embodiment aspect second of the present invention, in the step 2, culture with after carrier mixes, is carried out drying.
Described drying is preferably under 40-45 ° of C condition carries out, and is preferably 4-6 hour drying time.
In the preferred embodiment aspect second of the present invention, also comprise the step of screening and/or packing after step 2 is dry.
Described screening preferably uses vibratory sieve to carry out, more preferably 60-80 order vibratory sieve.
Third aspect of the present invention provides a kind of method of using described leavening to carry out cotton dregs or cottonseed cake fermentation, and step comprises:
Above-mentioned cotton cake toxicity removal microbe leaven is joined in cotton dregs or the cottonseed cake, ferment.
Wherein, the consumption of cotton cake toxicity removal microbe leaven is preferably the 1.0-0.25% of cotton dregs or cottonseed cake.
According to a kind of preferred embodiment of third aspect of the present invention, step comprises:
Above-mentioned cotton cake toxicity removal microbe leaven is joined in the substrate that contains cotton dregs or cottonseed cake, corn flour, wheat bran, water, ferment.
Wherein, the weight ratio of cotton dregs or cottonseed cake, corn flour, wheat bran, water is preferably (40-50): (1.5-3): (1.5-3): (40-70), be preferably (40-50): (2-3): (2-3): (40-60), more preferably 45: 2.5: 2.5: 50.
Fermentation temperature is preferably 25-38 ° of C, and 25-35 ° of C more preferably is such as 27-28 ° of C, 30 ° of C, 32-33 ° C.
Fermentation time is preferably 1-10 days, and more preferably 2-5 days, most preferably be 3-4 days, most preferably be 3 days.
According to a kind of preferred enforcement of third aspect of the present invention, after fermentation, also comprise the step with the tunning drying.
Wherein, described drying is preferably air-dry.
Wherein, described baking temperature is preferably 50-80 ° of C, 40-70 ° of C more preferably, and more preferably 50-60 ° of C most preferably is 60 ° of C.
Saccharomyces cerevisiae of the present invention, bacillus subtilis all can be bacterial classifications known and that can obtain by commercial sources, and concrete example is such as the preservation of Chinese agriculture microorganism fungus kind preservation administrative center and sell:
Bacillus subtilis (Bacillus subtilis) ACCC 10619
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC 20065
Of the present invention having the following advantages:
1, the selected bacterial classification of the present invention has inhibitory action to the disease bacterium, has avoided the pollution of miscellaneous bacteria in the cotton dregs sweat, has guaranteed product quality.The bacterial classification strong stress resistance of choosing is easy to the characteristics such as preservation.
2, the present invention's free gossypol in the cotton dregs of effectively degrading, degraded and softening crude fibre, improve its palatability, secretion and synthetic a large amount of active probiotics, nutrition and the hormonal substances such as bioactive micro peptide amino acid, various biochemical enzymes, somatomedin, animal is to wherein microbial bacteria body protein, the Effective Raise animal immunizing powers such as beta glucan.
3, a large amount of active probiotics of in the cotton cake toxicity removal sweat, producing of the present invention, can regulate the body gastrointestinal tract microecological balance and improve digestive enzyme activity, promote digestion, absorption and the utilization of feed nutrition, improve food conversion ratio and the price of deed, reduce ight soil stink and noxious gas emission.
The characteristics such as 4, the present invention has bacterial classification and zymotechnique is simple, and cost is low, and fermentation cotton dregs bacterial classification uses few, and fermentation cotton dregs cost is low, and market competition is obvious.
The specific embodiment
The invention will be further described for following examples.
Embodiment 1
The first step: thin saccharomyces cerevisiae (ACCC20065) bacterium is cultivated by potato culture (PDA), bacillus subtilis (ACCC10619) by nutrient agar cultivations of going down to posterity, is observed growth speed, 28 ℃ of cultivations 36 hours; With the saccharomyces cerevisiae bacterium colony of diameter greater than 2mm, diameter is preserved 4 ℃ of temperature greater than the bacterial strain of the bacillus subtilis bacterium colony of 5mm, and is for subsequent use;
Second step: the Wine brewing yeast strain that the first step is obtained is inoculated in the potato culture (PDA), the bacillus subtilis bacterial strain is seeded in the nutrient agar, be to cultivate 24 hours at 28 ℃ in 250 rev/mins the constant-temperature table at rotating speed respectively with two kinds of bacterial strains, sampling, utilize colony counting method to carry out total plate count and measure, total plate count greater than the bacterial classification of 2,000,000,000/ml for the production of the production of hybrid seeds;
The 3rd step: the bacillus subtilis, the S. cervisiae that filter out are transferred respectively in the eggplant bottle that nutrient agar is housed, under 28 ℃ of temperature, cultivated 46 hours, treat that eggplant bottle surface lawn is covered with, obtain S. cervisiae, bacillus subtilis for follow-up cultivation, then put into 4 ℃ refrigerator and preserve.
Saccharomyces cerevisiae 85 weight portions, bacillus subtilis 15 weight portions are mixed, with composite bacteria carry out that liquid at high speed is cultivated, solid enlarge cultivate after, the solid culture of gained is mixed with carrier, drying and crushing obtains microbe leaven.
Wherein, described liquid at high speed is cultivated: with fluid nutrient medium at 121 ℃ of high-temperature sterilization 15min, with composite bacteria by weight 1: 20 be inoculated in the aforesaid liquid culture medium, its rotating speed is 200r/min, 28-35 ℃ was stirred 28 hours, stopped to ferment when 2,000,000,000/ml concentration and pH value reach 6.8 when total viable count reaches.
Described carrier is cornstarch, and the mixed weight ratio of solid culture and carrier is 0.4: 1.
Described drying and crushing is: the mixture of solid culture and carrier is put into the incubator drying, temperature is controlled at 42,5 hours time, the material moisture content weight content that drying is finished≤12%, dried material is passed through 80 purpose reciprocating sieves, be packaged to be the microbe leaven finished product, total viable bacteria 2 * 10 in the leavening
9Cfu/g.
Microbe leaven finished product 1kg is joined cotton dregs: corn flour: wheat bran: water weight portion 45: 2.5: 2.5: in 1000 kilograms of substrates of 50 gross weights, mix, after ventilating under 30 ℃ of temperature 3 days, 60 ℃ get product after air-dry.
Table 1, the microbe leaven of embodiment 1 preparation is on the impact of cotton dregs nutrition
| ? | Before the fermentation | After the microbe leaven fermentation | Changing ratio % |
| Crude protein CP(%) | 37.28 | 40.06 | 7.46 |
| Free gossypol content mg/kg | 883 | 136 | -84.60 |
As can be seen from Table 1, use after the microbe leaven of the embodiment of the invention 1 preparation processes cotton dregs, crude protein content has improved 7.46%, the free gossypol content decrease 84.60%.
Embodiment 2
The first step: thin S. cervisiae (ACCC20065) is cultivated by potato culture (PDA), with bacillus subtilis (ACCC10619) by the nutrient agar cultivation of going down to posterity, observe growth speed, cultivate 36 hours with the saccharomyces cerevisiae bacterium colony of diameter greater than 2mm for 30 ℃, diameter is preserved 4 ℃ of temperature greater than the bacterial strain of the bacillus subtilis bacterium colony of 5mm;
Second step: the Wine brewing yeast strain that the first step is obtained is inoculated in the potato culture (PDA), the bacillus subtilis bacterial strain is seeded in the nutrient agar, be to cultivate 24 hours at 30 ℃ in 250 rev/mins the constant-temperature table at rotating speed respectively with two kinds of bacterial strains, sampling, utilize colony counting method to carry out total plate count and measure, total plate count greater than the bacterial classification of 2,000,000,000/ml for the production of the production of hybrid seeds;
The 3rd step: the bacillus subtilis, the S. cervisiae that filter out are transferred respectively in the eggplant bottle that nutrient agar is housed, under 30 ℃ of temperature, cultivated 48 hours, treat that eggplant bottle surface lawn is covered with, obtain S. cervisiae, bacillus subtilis for follow-up cultivation, then put into 4 ℃ refrigerator and preserve.
Saccharomyces cerevisiae 90 weight portions, bacillus subtilis 10 weight portions are mixed, with composite bacteria carry out that liquid at high speed is cultivated, solid enlarge cultivate after, the solid culture of gained is mixed with carrier, drying and crushing obtains microbe leaven.
Described liquid at high speed is cultivated: with fluid nutrient medium at 121 ℃ of high-temperature sterilization 20min, with composite bacteria by weight 1: 25 be inoculated in the aforesaid liquid culture medium, its rotating speed is 200-220r/min, 30 ℃ were stirred 30 hours, stopped to ferment when 2,000,000,000/ml concentration and pH value reach 7.2 when total viable count reaches.
Described carrier is cornstarch, and the mixed weight ratio of solid culture and carrier is 0.2: 1.
Described drying and crushing is: the mixture of solid culture and carrier is put into the incubator drying, temperature is controlled at 42 ℃, 4 hours time, the material moisture content weight content that drying is finished≤12%, dried material is passed through 80 purpose reciprocating sieves, be packaged to be the microbe leaven finished product, total viable bacteria 2 * 10 in the leavening
9Cfu/g.
Microbe leaven finished product 2kg is joined cotton dregs: corn flour: wheat bran: in 1000 kilograms of substrates of water weight portion 45:2.5:2.5:50 gross weight, mix, after ventilating under 30 ℃ of temperature 3 days, 60 ℃ get product after air-dry.
Table 2, the microbe leaven of embodiment 2 preparations is on the impact of cotton dregs nutrition
| ? | Before the fermentation | After the microbe leaven fermentation | Changing ratio % |
| Crude protein CP(%) | 37.28 | 40.58 | 8.85 |
| Free gossypol content mg/kg | 883 | 96 | -89.13 |
As can be seen from Table 2, use after the microbe leaven of the embodiment of the invention 2 preparations processes cotton dregs, crude protein content has improved 8.85%, the free gossypol content decrease 89.13%.
Table 3, the prepared microbe leavens fermentation cotton dregs of embodiment 2 are on the impact of amino acid content
| Numbering | Test item | Unit | Before the fermentation | After the fermentation |
| 1 | Aspartic acid | g/100g | 4.24 | 4.04 |
| 2 | Threonine | g/100g | 1.37 | 1.64 |
| 3 | Serine | g/100g | 1.87 | 1.73 |
| 4 | Glutamic acid | g/100g | 9.10 | 9.18 |
| 5 | Glycine | g/100g | 1.56 | 1.61 |
| 6 | Alanine | g/100g | 2.11 | 2.03 |
| 7 | Cystine | g/100g | 0.57 | 0.57 |
| 8 | Valine | g/100g | 0.93 | 0.96 |
| 9 | Methionine | g/100g | 0.33 | 0.42 |
| 10 | Isoleucine | g/100g | 1.53 | 1.62 |
| 11 | Leucine | g/100g | 2.70 | 2.93 |
| 12 | Tyrosine | g/100g | 1.02 | 1.32 |
| 13 | Proline | g/100g | 1.68 | 1.77 |
| 14 | Histidine | g/100g | 2.77 | 2.85 |
| 15 | Lysine | g/100g | 1.82 | 2.28 |
| 16 | Arginine | g/100g | 4.72 | 4.96 |
| 17 | Total amino acid content | g/100g | 38.32 | 39.91 |
Can find out by table 3, the microbe leaven of the present invention's preparation, can significantly improve amino acid whose content, the content of the lysine that especially lacks in the cotton dregs has improved more than 25%, methionine content has improved more than 27%, the lysine that adds in the time of can reducing cottonseed cake or cotton dregs as feedstuff and the amount of methionine.
In addition, the present invention adopts the combination of saccharomyces cerevisiae and bacillus subtilis, in leavening, add simultaneously cornstarch, leavening can be dissolved into rapidly in the water, the coefficient of dispersion is high, can significantly degrade cotton dregs free gossypol and crude fibre, the accessory substance after the simultaneously cotton dregs fermentation can be used as the organic fertilizer recycling.
The present invention only need use two kinds of bacterial classifications, and production cost is low.But should be understood that, those skilled in the art also can add other bacterial classification or additive as required, and to obtain required performance, this interpolation is also within protection scope of the present invention.
Above specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (10)
1. a cotton cake toxicity removal microbe leaven is characterized in that, the active bacterium of described microbe leaven comprises saccharomyces cerevisiae and bacillus subtilis.
2. cotton cake toxicity removal microbe leaven according to claim 1 is characterized in that, saccharomyces cerevisiae and bacillus subtilis part by weight are (80-90): (10-20).
3. cotton cake toxicity removal microbe leaven according to claim 1 is characterized in that, also comprises carrier, and carrier is cornstarch.
4. the described cotton cake toxicity removal microbe leaven of any one is characterized in that according to claim 1-3, and total viable bacteria content is 1 * 10
8-1 * 10
10Cfu/g.
5. one kind prepares the as claimed in claim 1 method of cotton cake toxicity removal microbe leaven, it is characterized in that, step comprises:
Step 1 will comprise that the active bacterium of saccharomyces cerevisiae, bacillus subtilis mixes, cultivate,
Step 2 is mixed culture with carrier, obtain described microbe leaven.
6. method according to claim 5, it is characterized in that, in the step 1, described cultivation comprises that liquid at high speed is cultivated and solid afterwards enlarges cultivation, the method that described liquid at high speed is cultivated is: the active bacterium that will mix is inoculated in the fluid nutrient medium, to total viable count 〉=100,000,000/ml concentration, and the pH value stops fermentation when reaching 6.5-7.5.
7. method according to claim 6 is characterized in that, during described liquid at high speed is cultivated, with the active bacterium that mixes according to weight ratio 1: (20-25) be inoculated in the fluid nutrient medium, cultivation temperature is 25-38 ° of C.
8. method according to claim 5 is characterized in that, described carrier is solid carrier, and the part by weight of culture and carrier is (0.1-1) in the step 2: 1.
9. a right to use requires 1 described leavening to carry out the method for cotton dregs or cottonseed cake fermentation, it is characterized in that, step comprises: above-mentioned cotton cake toxicity removal microbe leaven is joined in cotton dregs or the cottonseed cake, ferment, the consumption of cotton cake toxicity removal microbe leaven is the 1.0-0.25% of cotton dregs or cottonseed cake.
10. method according to claim 9 is characterized in that, step comprises: above-mentioned cotton cake toxicity removal microbe leaven is joined in the substrate that contains cotton dregs or cottonseed cake, corn flour, wheat bran, water, ferment; Wherein, the weight ratio of cotton dregs or cottonseed cake, corn flour, wheat bran, water is (40-50): (1.5-3): (1.5-3): (40-70).
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|---|---|---|---|---|
| CN104256058A (en) * | 2014-04-16 | 2015-01-07 | 江苏盐城源耀生物科技有限公司 | Method of producing feed having high small-peptide content by biological fermentation of cottonseed meal |
| CN104328063A (en) * | 2014-06-27 | 2015-02-04 | 安徽农业大学 | Gossypol degradation strain coming from ruminant rumens and application thereof |
| CN104342471A (en) * | 2014-10-10 | 2015-02-11 | 哈尔滨艾克尔食品科技有限公司 | Method for preparing dephenolized cottonseed protein peptide |
| CN104438293A (en) * | 2014-11-27 | 2015-03-25 | 苏州嘉禧萝生物科技有限公司 | Microbial detoxification method of cottonseed cakes |
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| CN113416673A (en) * | 2021-06-21 | 2021-09-21 | 西北民族大学 | Complex microbial inoculant for detoxifying cottonseed meal as well as preparation method and application thereof |
| CN115152892A (en) * | 2022-09-07 | 2022-10-11 | 北京挑战农业科技有限公司 | Method for degrading free gossypol in cottonseed meal, product and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444252A (en) * | 2008-11-18 | 2009-06-03 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN101692868A (en) * | 2009-10-20 | 2010-04-14 | 大连工业大学 | Method for producing animal feed based on cottonseed meal physical, chemical and microbial detoxification technology |
| CN102178035A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains |
| CN102640889A (en) * | 2012-05-21 | 2012-08-22 | 薛松晓 | Detoxifying agent for cottonseed meal and rapeseed meal as well as preparation method thereof and preparation method of microorganism feed prepared by detoxifying agent |
-
2013
- 2013-01-21 CN CN2013100211192A patent/CN103355472A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444252A (en) * | 2008-11-18 | 2009-06-03 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN101692868A (en) * | 2009-10-20 | 2010-04-14 | 大连工业大学 | Method for producing animal feed based on cottonseed meal physical, chemical and microbial detoxification technology |
| CN102178035A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains |
| CN102640889A (en) * | 2012-05-21 | 2012-08-22 | 薛松晓 | Detoxifying agent for cottonseed meal and rapeseed meal as well as preparation method thereof and preparation method of microorganism feed prepared by detoxifying agent |
Non-Patent Citations (7)
| Title |
|---|
| 中国农科院饲料研究所技术转移中心: "用生物发酵的方法提升棉籽饼粕的营养价值", 《饲料与畜牧》 * |
| 刘建成等: "复合菌固态发酵棉籽粕工艺的初步研究", 《饲料工业》 * |
| 张庆华等: "三菌株协同固态发酵对棉粕脱毒效果及其生物活性的影响", 《饲料工业》 * |
| 张文举等: "假丝酵母ZD-3与黑曲霉ZD-8复合固体发酵对棉籽饼脱毒及营养价值的影响研究", 《中国粮油学报》 * |
| 汤江武等: "响应面法与基于神经网络的遗传算法在优化棉粕固态发酵中外源添加物用量上的应用", 《浙江大学学报（农业与生命科学版）》 * |
| 葛洪等: "多菌种混合固体发酵底物对棉籽粕脱毒效果的研究", 《扬州大学学报（农业与生命科学版）》 * |
| 金红春等: "混合菌剂固态发酵棉粕脱毒条件的优化", 《中国农学通报》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN104328063A (en) * | 2014-06-27 | 2015-02-04 | 安徽农业大学 | Gossypol degradation strain coming from ruminant rumens and application thereof |
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| CN107173544A (en) * | 2017-06-07 | 2017-09-19 | 江苏久久和牧农牧科技有限公司 | A kind of production method of miscellaneous dregs of rice fermented feed |
| CN110150451A (en) * | 2017-11-30 | 2019-08-23 | 浙江省农业科学院 | A kind of method that bacterium enzyme collaboration glycolysis produces the low gossypol high quality cotton benevolence of full-cream high energy |
| CN108813289A (en) * | 2018-06-01 | 2018-11-16 | 湖北华大瑞尔科技有限公司 | A kind of preparation and the preparation method and application thereof of high-performance bio degradation mycotoxin |
| CN108813289B (en) * | 2018-06-01 | 2022-05-17 | 湖北华大瑞尔科技有限公司 | Application method and preparation method of preparation for efficiently biodegrading mycotoxin |
| CN112369499A (en) * | 2020-10-21 | 2021-02-19 | 珠海市德海生物科技有限公司 | Feed additive, sea bass feed containing high dephenolized cottonseed protein and preparation method of sea bass feed |
| CN113416673A (en) * | 2021-06-21 | 2021-09-21 | 西北民族大学 | Complex microbial inoculant for detoxifying cottonseed meal as well as preparation method and application thereof |
| CN115152892A (en) * | 2022-09-07 | 2022-10-11 | 北京挑战农业科技有限公司 | Method for degrading free gossypol in cottonseed meal, product and application thereof |
| CN115152892B (en) * | 2022-09-07 | 2022-11-25 | 北京挑战农业科技有限公司 | Method for degrading free gossypol in cottonseed meal, product and application thereof |
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