CN104256058A - Method of producing feed having high small-peptide content by biological fermentation of cottonseed meal - Google Patents
Method of producing feed having high small-peptide content by biological fermentation of cottonseed meal Download PDFInfo
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- CN104256058A CN104256058A CN201410153458.0A CN201410153458A CN104256058A CN 104256058 A CN104256058 A CN 104256058A CN 201410153458 A CN201410153458 A CN 201410153458A CN 104256058 A CN104256058 A CN 104256058A
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- cotton dregs
- fermentation
- lactobacillus acidophilus
- biofermentation
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Abstract
The invention discloses a method of producing feed having a high small-peptide content by biological fermentation of cottonseed meal, and belongs to the technical field of biological fermentation. The method adopts the cottonseed meal as a raw material. A lactobacillus acidophilus liquid and a yeast seed liquid are added. The material-liquid ratio is 1:0.6-1:0.9. Solid-state fermented cottonseed meal is obtained by performing anaerobic fermentation at 30-50 DEG C for 3-4 days, drying and smashing. The method adopts solid state fermentation, and is simple in process and low in cost. A finished product of the fermented cottonseed meal is reddish, is rich in lactic acid, various digestive enzyme, vitamins and small peptides, and has a large amount of probiotics and rich and mellow fragrance of distiller's yeast. Oxidation resistance of the product is largely enhanced that of the raw material. After fermentation, the content of gossypol is lower than 50 mg/Kg, and main mycotoxins are not detected. The feed is capable of promoting animal ingestion and boosting animal immunity and is safe and absorbable.
Description
Technical field:
The present invention relates to the method utilizing biofermentation cotton dregs to produce high small peptide feed, be specifically related to utilize lactobacillus acidophilus and saccharomycetes to make fermentation cotton dregs to produce a kind of food calling, urge digestion, improved the method that the biofermentation cotton dregs of the biofermentation products of immunity and detoxification produce high small peptide feed.
Background technology:
Cotton dregs are face cakes that cottonseed draws after squeezing, then separate through most of Residual oil of extract technology by the inside, a kind of micro-red or yellow granular article obtained, and China's cotton dregs annual production is about 500 ~ 7,000,000 tons, and source is wide, and cost is low.Because cotton dregs contain a large amount of ANFs, be not utilized effectively.Feedstuff industry is used at present mainly as primary raw materials.
Cotton dregs are rich in abundant nutriment, comprise protein, crude fat, SNSP etc.But simultaneously also containing phytic acid, raffinose, gossypol and cyclopropene fatty acid, these composition influence palatabilities or have animal endanger significantly, if carried out biodegradation, just can overcome above-mentioned defect, improve the disease-resistant effect of health care simultaneously, will greatly promote the raising of China's cultivation level undoubtedly.
Cotton dregs contain rich in protein, up to 41%, the quality of albumen is similar to dregs of beans, but is mostly indissoluble, is difficult to the albumen of digestibility and utilization, changed into the little peptide or amino acid that are easy to digest in fermentation process, greatly improved the absorption of animal to protein.
Cotton dregs are also containing a large amount of SNSPs, and SNSP mostly is ANFs and is is not easily digested and assimilated by animal except cellulose, adds the small molecular sugar that its catalytic decomposition can become can be utilized by corresponding non-starch enzyme.
Cotton dregs are also containing a large amount of raffinose, and raffinose is ANFs, and the raffinose utilization that can be decomposed in biological fermentation process is fallen.
Cotton dregs also have a large amount of phytic acid, and phytic acid have impact on the absorption of animal to protein, mineral matter, produces phytase in fermentation process, and provide good catalytic environment for phytase, and phytic acid content is greatly reduced.
Cotton dregs are also containing a large amount of gossypol, and gossypol easily causes animal poisoning, wherein most gossypol can be degraded into nontoxic composition, thus successfully carry out detoxification to cotton dregs in fermentation process.
Lactobacillus-fermented can produce a large amount of lactic acid, lactobacillus acidophilus is homofermentative lactic, the existence of acid can improve the palatability of feed greatly, the feed intake saccharomycete improving animal can grow in acid condition fast, saccharomycete and metabolite thereof have strong distiller's yeast fragrance and toast perfume, add attractant.
The sweat of lactobacillus acidophilus can produce a large amount of biocidal property material, at the collaborative lower bacteriostasis that effectively can improve inhibitory substance of lactic acid.Promote gut flora balance, reduce animal intestinal disease and diarrhoea.
Another problem of cotton dregs easily there is mycotoxin, biofermentation not only can degrade part mycotoxin, as aflatoxin, vomitoxin, and thalline itself also has suction-operated to mycotoxin, improves the security of feed.
The domestic and international deep development for cotton dregs application at present mainly concentrates on detoxification aspect, as the culture medium of certain product, or as the little auxiliary material of biological fermentation feed.
Not yet find at present to utilize lactobacillus acidophilus and saccharomycete to carry out the patent of fermentation aspect to cotton dregs, and not yet someone carries out the research in lactic acid bacteria and saccharomycetes to make fermentation cotton dregs lactic acid producing, oxidation resistance, how little peptide, attractant at present.
Summary of the invention:
The object of the invention is with cotton dregs is raw material, utilize lactobacillus acidophilus to become a kind of with saccharomycetes to make fermentation there is excellent attractant, enrich lactic acid content, excellent anti-oxidation characteristics, compared with higher primary school's peptide content, the method for producing high small peptide feed compared with the biofermentation cotton dregs of the fermentation cotton dregs of multi-probiotics.
Object of the present invention can be achieved through the following technical solutions:
Biofermentation cotton dregs produce the method for high small peptide feed, are take cotton dregs as raw material, add zymotic fluid, glucose, enzyme preparation and water and carry out temperature controlled fermentation, ferment 3 ~ 4 days, namely obtain excellent fermentation cotton dregs after drying.Concrete steps are as follows:
1. the process of raw material: pulverizing is carried out to cotton dregs and obtains cotton dregs crushed material;
2. mixing is inoculated: the cotton dregs crushed material 1. obtained to step adds zymotic fluid, adding proportion is 15% ~ 30% of cotton dregs crushed material quality, add after glucose, enzyme preparation and water mix and obtain the front material that ferments, wherein the adding proportion of glucose is 10% ~ 12% of cotton dregs crushed material quality, enzyme preparation adding proportion be 0.1 ‰ ~ 0.3 ‰ of cotton dregs crushed material quality, final material-water ratio 1: 0.6 ~ 1: 0.9;
3. temperature controlled fermentation: fermentation forward slip value thing step 2. obtained inserts fermenting tank for fermentation, 30 ~ 50 DEG C of temperature controlled fermentations 3 ~ 4 days;
4. dry pulverizing: 60 DEG C of oven dry, pulverize;
5. bag is filled.
In a preferred embodiment of the invention, described step 1. in, cotton dregs are the cotton dregs that sterilizing or non-sterilization treatment are crossed.
In a preferred embodiment of the invention, described step 2. in, described zymotic fluid is mixed by lactobacillus acidophilus liquid and saccharomycete seed liquor, and mass ratio is between the two 10: 1; Described lactobacillus acidophilus is through Chinese microorganism strain preservation center preservation, and strain number: CGMCC NO.8043, wherein in lactobacillus acidophilus liquid, lactobacillus acidophilus cell concentration reaches 4 × 10
8~ 9 × 10
8cfu/mL, in saccharomycete seed liquor, saccharomycete cell concentration reaches 1 × 10
8~ 3 × 10
8cfu/mL;
In a preferred embodiment of the invention, described step 2. in, final material-water ratio is 1: 0.9.
In a preferred embodiment of the invention, described step 2. in, described enzyme preparation is one or more the mixing in protease, mannonase zytase.
The present invention compared with prior art, has following innovation and beneficial functional:
1. cotton dregs utilize lactobacillus acidophilus can produce abundant lactic acid and a large amount of biocidal property material, and lactic acid content reaches 6% one 10%, and lactic acid has food calling effect concerning pig, also can kill simultaneously or suppress pathogenic bacteria, and useful animals is healthy.
2. in sweat, indissoluble, heavy protein can be degraded to the little peptide easily absorbed, and content reaches 20%, and content is very abundant, and it is not easily saturated that little peptide has carrier, promote the features such as Amino Acid Absorption, absorb very helpful to animal.
3. lactobacillus acidophilus and saccharomycetes to make fermentation cotton dregs product have strong fragrance, the ester class produced in sweat, organic acid, and the fragrance produced in drying course can have good food calling effect to animal.
4. the gossypol in cotton dregs can be degraded into nontoxic composition by lactobacillus acidophilus and saccharomycete in the process of fermentation cotton dregs, is reached for the object of cotton cake toxicity removal.
5. fermented product oxidation resistance comparatively cotton dregs had and significantly promoted, TAC rises to 2.88 by 0.66 of cotton dregs raw material, not only can remove free radical unnecessary in animal body, also powerful to animal immune capability improving.
6. ferment cotton dregs also containing a large amount of probio, wherein lactobacillus acidophilus has very many effects to animal health, and as suppressed pathogenic bacteria, absorption pathogenic bacteria also bring to external etc.
7. fermentation can also reduce or remove some ANFs in cotton dregs, as removed phytic acid, raffinose, and degraded SNSP etc.
8. also have some UGFs in sweat in addition, can growth of animal be promoted.
Detailed description of the invention
Following examples are only to explanation of the present invention, do not form the restriction to the claims in the present invention protection domain.
Unless expressly stated otherwise, the zymotic fluid used mixes by adopting lactobacillus acidophilus liquid and saccharomycete seed liquor following examples, and mass ratio is between the two 10:1; Lactobacillus acidophilus is through Chinese microorganism strain preservation center preservation, and strain number: CGMCC NO.8043, wherein in lactobacillus acidophilus liquid, lactobacillus acidophilus cell concentration reaches 4 × 10
8~ 9 × 10
8cfu/mL, in saccharomycete seed liquor, saccharomycete cell concentration reaches 1 × 10
8~ 3 × 10
8cfu/mL;
Embodiment 1:
Biofermentation cotton dregs produce the method for high small peptide feed, the steps include: cotton dregs 100Kg, unsterilised, add the mixed liquor of 0.1 ‰ zytases, 0.1 ‰ mannonase 30Kg lactobacillus acidophilus and saccharomycete seed liquor, 10Kg glucose and 60Kg running water, mix, put into fermentation vat temperature controlled fermentation, 44 DEG C ferment 80 hours, dry pulverizing dress bags for 60 DEG C and get product.
Product is in partially red, and have fragrance, pH4.3, gossypol content 80mg/kg, little peptide content 9.1%, lactic acid content 7.1%, viable count reaches 3*10^7cfu/g.
Embodiment 2:
Biofermentation cotton dregs produce the method for high small peptide feed, the steps include: cotton dregs 100Kg, 100 DEG C of sterilizings 10 minutes, add the mixed liquor of 0.2 ‰ protease (percentage by weight), 25Kg lactobacillus acidophilus and saccharomycete seed liquor, 10Kg glucose and 65Kg running water, mix, put into fermentation vat temperature controlled fermentation, 32 DEG C ferment 80 hours, dry pulverizing dress bags for 60 DEG C and get product.
Product is partially red, has fragrance, pH4.5, gossypol content 60mg/kg, and little peptide content 16%, lactic acid content 6.5%, viable count reaches 2*10^7cfu/g.
Embodiment 3:
Biofermentation cotton dregs produce the method for high small peptide feed, the steps include: cotton dregs 100Kg, unsterilised, add the mixed liquor of 0.2 ‰ protease (percentage by weight), 0.1 ‰ zytases, 0.1 ‰ mannonase 10Kg glucose, 20Kg lactobacillus acidophilus and saccharomycete seed liquor and 70Kg running water, mix, put into fermentation vat temperature controlled fermentation, 40 DEG C ferment 80 hours, dry pulverizing dress bags for 60 DEG C and get product.
Product is partially red, has fragrance, pH3.9, gossypol content 40mg/kg, and little peptide content 19%, lactic acid content 10.2%, viable count reaches 5.2*10^7cfu/g
Embodiment 4:
Biofermentation cotton dregs produce the method for high small peptide feed, the steps include: cotton dregs 100Kg, add the mixed liquor of 0.1 ‰ protease (percentage by weight), 0.1 ‰ mannonase 10Kg glucose, 15Kg lactobacillus acidophilus and saccharomycete seed liquor and 75Kg running water, mix, put into fermentation vat temperature controlled fermentation, 30 DEG C ferment 80 hours, dry pulverizing dress bags for 60 DEG C and get product.
Product is partially red, has fragrance, pH4.1, gossypol content 80mg/kg, gossypol content 70mg/kg, and little peptide content 6.1%, lactic acid content 9.1%, viable count reaches 4.9*10^7cfu/g.
Claims (5)
1. biofermentation cotton dregs produce the method for high small peptide feed, it is characterized in that, are take cotton dregs as raw material, add zymotic fluid, glucose, enzyme preparation and water and carry out temperature controlled fermentation, ferment 3 ~ 4 days, namely obtain fermentation cotton dregs after drying; Concrete steps are as follows:
1. the process of raw material: pulverizing is carried out to cotton dregs and obtains cotton dregs crushed material;
2. mixing is inoculated: the cotton dregs crushed material 1. obtained to step adds zymotic fluid, adding proportion is 15% ~ 30% of cotton dregs crushed material quality, add after glucose, enzyme preparation and water mix and obtain the front material that ferments, wherein the adding proportion of glucose be 10% ~ 12% enzyme preparation of cotton dregs crushed material quality adding proportion be 0.1 ‰ ~ 0.3 ‰ final material-water ratios 1: 0.6 ~ 1: 0.9 of cotton dregs crushed material quality;
3. temperature controlled fermentation: fermentation forward slip value thing step 2. obtained inserts fermenting tank for fermentation, 30 ~ 50 DEG C of temperature controlled fermentations 3 ~ 4 days;
4. dry pulverizing: 60 DEG C of oven dry, pulverize;
5. bag is filled.
2. biofermentation cotton dregs as claimed in claim 1 produce the method for high small peptide feed, it is characterized in that, described step 1. in, cotton dregs are the cotton dregs that sterilizing or non-sterilization treatment are crossed.
3. biofermentation cotton dregs as claimed in claim 1 produce the method for high small peptide feed, it is characterized in that, described step 2. in, described zymotic fluid is mixed by lactobacillus acidophilus liquid and saccharomycete seed liquor, and mass ratio is between the two 10: 1; Described lactobacillus acidophilus is through Chinese microorganism strain preservation center preservation, and strain number: CGMCC NO.8043, wherein in lactobacillus acidophilus liquid, lactobacillus acidophilus cell concentration reaches 4 × 10
8~ 9 × 10
8cfu/mL, in saccharomycete seed liquor, saccharomycete cell concentration reaches 1 × 10
8~ 3 × 10
8cfu/mL.
4. biofermentation cotton dregs as claimed in claim 1 produce the method for high small peptide feed, it is characterized in that, described step 2. in, final material-water ratio is 1: 0.9.
5. biofermentation cotton dregs as claimed in claim 1 produce the method for high small peptide feed, it is characterized in that, described step 2. in, described enzyme preparation is one or more the mixing in protease, mannonase zytase.
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|---|---|---|---|
| CN201410153458.0A CN104256058A (en) | 2014-04-16 | 2014-04-16 | Method of producing feed having high small-peptide content by biological fermentation of cottonseed meal |
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| CN201410153458.0A CN104256058A (en) | 2014-04-16 | 2014-04-16 | Method of producing feed having high small-peptide content by biological fermentation of cottonseed meal |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105755079A (en) * | 2016-01-28 | 2016-07-13 | 江苏盐城源耀生物科技有限公司 | Method for preparing flavored soybean protein concentrate |
| CN109043123A (en) * | 2018-10-23 | 2018-12-21 | 河南正本清源科技发展股份有限公司 | The preparation method and application of fermented feed, fermented feed |
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| CN102246884A (en) * | 2010-05-20 | 2011-11-23 | 上海源耀生物科技有限公司 | Fermented protein feed, preparation method thereof and legehenne protein feed containing same |
| CN102805208A (en) * | 2012-09-06 | 2012-12-05 | 新疆希普生物科技股份有限公司 | Preparation method of high-lysine fermentation and detoxification cottonseed meal |
| CN103355472A (en) * | 2013-01-21 | 2013-10-23 | 上海创博生态工程有限公司 | Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof |
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2014
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Patent Citations (3)
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| CN102246884A (en) * | 2010-05-20 | 2011-11-23 | 上海源耀生物科技有限公司 | Fermented protein feed, preparation method thereof and legehenne protein feed containing same |
| CN102805208A (en) * | 2012-09-06 | 2012-12-05 | 新疆希普生物科技股份有限公司 | Preparation method of high-lysine fermentation and detoxification cottonseed meal |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105755079A (en) * | 2016-01-28 | 2016-07-13 | 江苏盐城源耀生物科技有限公司 | Method for preparing flavored soybean protein concentrate |
| CN109043123A (en) * | 2018-10-23 | 2018-12-21 | 河南正本清源科技发展股份有限公司 | The preparation method and application of fermented feed, fermented feed |
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Application publication date: 20150107 |