CN104381587A - Biological processing method for probiotics cottonseed polypeptide - Google Patents
Biological processing method for probiotics cottonseed polypeptide Download PDFInfo
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- CN104381587A CN104381587A CN201410153459.5A CN201410153459A CN104381587A CN 104381587 A CN104381587 A CN 104381587A CN 201410153459 A CN201410153459 A CN 201410153459A CN 104381587 A CN104381587 A CN 104381587A
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Abstract
The invention discloses a biological processing method for probiotics cottonseed polypeptide. According to the method, an excellent-quality cottonseed protein product is obtained by utilizing bacillus subtilis and an enzyme preparation to process cottonseed protein and fermenting at 30 DEG C for 2-3 d. The cottonseed protein product has the free gossypol content lower than the safe standard prescribed by the forage, the crude protein content of 50%, the small peptide content of 25%, the oxidation resistance improved by 53% and substantially-increased free amino acid content, and can be directly used as a protein forage. The biological processing method relatively well solves the problems that a product produced by employing a prior art is bad in sensory quality and bad in palatability and the protein quality becomes bad, is a cottonseed protein biological processing method which is capable of substantially improving nutrition value and oxidation resistance, is low in energy consumption and also enables the cottonseed protein to reach the forage standards, and has wide application prospect.
Description
Technical field
The present invention relates to a kind of biological processing method utilizing micro-raw preparation and enzyme preparation associated treatment cottonseed flour, particularly a kind of biological processing method of prebiotic cotton-seed polypeptide.
Background technology
Shi Chanmian big country of China, output of cotton occupies first place in the world, and produces nearly 9,000,000 tons of the Cottonseed Meal that can be used for protein resource per year, accounts for 10.7% of albumen dregs of rice total amount.Current cottonseed protein series products mainly comprises Cottonseed Meal and cottonseed flour, the high-quality protein resource about containing more than 40% in Cottonseed Meal, but this huge protein resource is but because it contains toxin-gossypol, limits its a large amount of utilizations in feed.
Cottonseed flour is a kind of protide product newly developed at present, and its protein content is up to more than 50%, and its gossypol content is well below Cottonseed Meal simultaneously, and crude fiber content is also well below Cottonseed Meal, and its feature determines can add use in a large number in feed.
Cottonseed protein is primarily of the globulin of 90% and the glutelin composition of 10%, and globulin mainly contains 2S, 7S, 11S tri-kinds of unit structures, the amino acid composition of cottonseed protein has good balance characteristic compared with FAO standard, and with strong complementarity with dregs of beans, is a kind of excellent protein resource.
Everybody mainly concentrates on the following aspects to the utilization of cottonseed protein at present: 1. for milk cow forage, research proves that cottonseed flour is used for milk cow forage, the highest alternative 50% dregs of beans, also can improve milk production of cow, improve dairy products quality while reducing costs.2. prepare Edible protein flour, cottonseed protein can obtain the albumen powder of more than 90% through separation and purification, and is easily absorbed by the body containing the little peptide of high level.3. enzyme-squash techniqued higher primary school peptide prod, mainly utilize alkali protease and flavor protease to carry out enzyme-squash techniqued higher primary school peptide product to cottonseed flour, but enzymolysis easily produces bitter peptides, during feeding animals, palatability is bad, can reduce animal feed intake.
Chinese patent (CN200710097709, CN200610090587) discloses two kinds and utilizes enzyme preparation to process process cottonseed flour to obtain the researchs of antioxidation active peptides, but its exist efficiency low, easily produce the shortcoming such as bitter peptides, function singleness.
Summary of the invention
The problem that the object of this invention is to provide a kind of biological processing method of prebiotic cotton-seed polypeptide, adopt the method mainly to solve to improve cottonseed protein digestibility, improve little peptide productive rate and protein hydrolysis degree, improve cottonseed protein palatability, improve animal disease resistant for feed.
Object of the present invention is realized by following steps:
A biological processing method for prebiotic cotton-seed polypeptide, specifically comprises the steps:
(1) process of raw material: sterilizing or non-sterilization treatment are carried out to cottonseed flour and obtains the cottonseed flour after processing;
(2) preparation of withered grass gemma seed liquor: use bacillus subtilis (Bacillus subtilis) to adopt medium culture to obtain withered grass gemma seed liquor after 12 hours, the cell concentration of described withered grass gemma seed liquor reaches 10
9-10
10cfu/mL;
(3) preparation of mixed liquor is inoculated
Withered grass gemma seed liquor, enzyme preparation and the water step (2) prepared mix and obtain inoculating mixed liquor; Wherein the mass percent of withered grass gemma seed liquor is 17 ~ 25%, and the mass percent of enzyme preparation is 0.30 ~ 0.50%, and the mass percent of water is 75 ~ 83%;
(4) inoculation mixing: add in the cottonseed flour after the process of step (1) by inoculation mixed liquor prepared by step (3), obtains fermentation forward slip value thing after mixing; Material water quality between inoculation mixed liquor prepared by the cottonseed flour after the process of wherein step (1) and step (3) is than being 1:0.5 ~ 1:1.5;
(5) temperature controlled fermentation: the fermentation forward slip value thing of step (4) is inserted fermenting tank for fermentation, underlying ventilated environment, and thickness of feed layer controls at 10-30cm, temperature controlled fermentation 2 ~ 3 days; Prebiotic cotton-seed polypeptide fermentate is obtained after fermentation ends;
(6) pulverizing is dried: prebiotic cotton-seed polypeptide fermentate step (5) obtained is dried, pulverize, fill bag obtains wanted product.
In a preferred embodiment of the invention, the bacterial classification of described bacillus subtilis (Bacillus subtilis) sieves from wild environment, its strain number is BS13002, deposit number is CGMCCNO.8745, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, January 20 2014 preservation time, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
In a preferred embodiment of the invention, described culture medium contains the material of following mass percent: glucose 1.5%, peptone 1%, beef extract 1%, potassium chloride 0.3 ‰, calcium chloride 0.7 ‰, magnesium sulfate 0.2 ‰, and the pH value of culture medium is 7.0.
In a preferred embodiment of the invention, in described step (1), the condition of cottonseed flour being carried out to sterilizing is 100 ~ 120 DEG C, sterilization time 10 ~ 30 minutes.
In a preferred embodiment of the invention, in described step (4), the material water quality between inoculation mixed liquor prepared by the cottonseed flour after the process of described step (1) and step (3) compares 1:0.9.
In a preferred embodiment of the invention, in described step (4), mix rear adjustment pH to 7.2.
In a preferred embodiment of the invention, in described step (5), fermentation temperature is 25 ~ 50 DEG C.
In a preferred embodiment of the invention, in described step (6), bake out temperature is 60-80 DEG C.
In preferably implementing at one of the present invention, described enzyme preparation is one or more the mixing in acid protease, neutral proteinase, alkali protease, flavor protease.
The present invention adopts bacterium enzyme associated treatment cottonseed flour, has following beneficial effect: 1. the protease that produces of microorganism and external enzyme preparation can associated treatment high molecular weight proteins, are degraded to the little peptide easily absorbed.2. microbial action reduces the generation of bitter peptides in albumen, can produce some flavor substances simultaneously, its palatability is increased.3. bacillus subtilis itself is probio, can secrete antibacterial peptide and suppress pathogenic bacterial infection, can consume enteron aisle oxygen, promote the growth of Intestinal Anaerobic Bacteria, can the growing of immune stimulatory organ, and a large amount of digestive ferment and vitamin can be secreted, promote that animal is to the absorption of nutriment.
Core of the present invention is bacterium enzyme associated treatment cottonseed protein product, fermentable and enzymatic hydrolysis condition are had complementary advantages, and products obtained therefrom has the feature of high protein (more than 50%), higher primary school's peptide (more than 25%), anti-oxidant, disease resistance (suppressing pathogenic bacteria), short search for food (fermentation produces flavor substance), absorption easy to digest.
The present invention compared with prior art, has following innovation and beneficial functional:
1. high protein, higher primary school's peptide, high digestibility: prebiotic cotton-seed polypeptide contains the protein of more than 50%, the little peptide content of more than 25%, pole is beneficial to animal and absorbs, bacillus subtilis simultaneously can produce a large amount of digestive enzymes, in feed or enter in animal body, promotes digesting and assimilating of animal.
2. bacterium enzyme associated treatment, have complementary advantages: fermentable temperature is also ferment treatment optimum temperature simultaneously, protease processing site is complementary, humidification effect.
3. increase feed palatability: the bitter peptides that enzyme preparation effect produces, the peptide ending enzyme effect that can be produced by microorganism, is degraded to amino acid, not only controls bitter taste, and the flavor substance that fermentable produces also can promote searching for food of animal.
4. fermented product oxidation resistance comparatively cottonseed flour had and significantly promoted, TAC rises to 2.37 by 1.52 of cottonseed protein powder raw material, not only can remove free radical unnecessary in animal body, also powerful to animal immune capability improving.
5. improve animal disease resistant: bacillus subtilis not only can produce some antibacterial peptides, suppress the opportunistic bacteria growing of pathogenic bacteria or autogenous infection; Free oxygen in rapid consumption environment, causes enteron aisle hypoxemia, promotes that useful anaerobic bacteria grows, and produces the organic acids such as lactic acid, reduces gut pH, indirectly suppresses the growth of other pathogenic bacteria; Growing of stimulating animal immune organ, activates T, bone-marrow-derived lymphocyte, improves immunoglobulin (Ig) and antibody horizontal, strengthens cellular immunity and humoral immune function, improves herd immunity.
6. reduce or remove some ANFs in cottonseed flour, as removed phytic acid, softening fibre element etc.
7. also have some UGFs in sweat in addition, can growth of animal be promoted.
Detailed description of the invention
Following examples are only to explanation of the present invention, do not form the restriction to the claims in the present invention protection domain.
Following examples unless expressly stated otherwise, its bacillus subtilis is sieved from wild environment for applicant, its strain number is BS13002, and deposit number is CGMCC NO.8745, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The bacillus subtilis seed liquor that following examples use is obtained after 12 hours through medium culture by bacillus subtilis, and the cell concentration of bacillus subtilis seed liquor reaches 10
9-10
10cfu/mL.
Culture medium contains the material of following mass percent: glucose 1.5%, peptone 1%, beef extract 1%, potassium chloride 0.3 ‰, calcium chloride 0.7 ‰, magnesium sulfate 0.2 ‰, and the pH value of culture medium is 7.0.
Embodiment 1:
Cottonseed flour 100kg, 110 DEG C of sterilizings 10 minutes, add the inoculation mixed liquor of 20kg bacillus subtilis seed liquor, 0.2kg alkali protease, 0.1kg flavor protease and 70kg running water preparation, mix, adjust ph is 7.2, puts into fermentation vat temperature controlled fermentation, thickness of feed layer 20cm, underlying ventilated environment, 30 DEG C ferment 60 hours, dry pulverizing dress bags for 60 DEG C and get product.
Product, in yellow, has fragrance, crude protein 53.3%, little peptide content 26.9%, and viable count reaches 3*10^8/g, and oxidation resistance promotes 51%.
Embodiment 2:
Cottonseed flour 100kg, 120 DEG C of sterilizings 5 minutes, add the inoculation mixed liquor of 15kg bacillus subtilis seed liquor, 0.2kg alkali protease, 0.2kg flavor protease and 65kg running water preparation, mix, adjust ph is 7.2, puts into fermentation vat temperature controlled fermentation, thickness of feed layer 15cm, underlying ventilated environment, 37 DEG C ferment 72 hours, dry pulverizing dress bags for 70 DEG C and get product.
Product, in yellow, has fragrance, crude protein 51.2%, little peptide content 26.9%, and viable count reaches 1*10^8/g, and oxidation resistance promotes 63%.
Embodiment 3:
Cottonseed flour 100kg, 105 DEG C of sterilizings 15 minutes, add the inoculation mixed liquor of 15kg bacillus subtilis seed liquor, 0.2kg alkali protease, 0.1kg flavor protease and 75kg running water preparation, mix, adjust ph is 7.2, puts into fermentation vat temperature controlled fermentation, thickness of feed layer 30cm, underlying ventilated environment, 43 DEG C ferment 72 hours, dry pulverizing dress bags for 75 DEG C and get product.
Product, in yellow, has fragrance, crude protein 52.9%, little peptide content 24.8%, and viable count reaches 6*10^7/g, and oxidation resistance promotes 55%.
Embodiment 4:
Cottonseed flour 100kg, 110 DEG C of sterilizings 10 minutes, add the inoculation mixed liquor of 20kg bacillus subtilis seed liquor, 0.2kg alkali protease, 0.1kg flavor protease and 60kg running water preparation, mix, adjust ph is 7.2, puts into fermentation vat temperature controlled fermentation, thickness of feed layer 25cm, underlying ventilated environment, 30 DEG C ferment 60 hours, dry pulverizing dress bags for 70 DEG C and get product.
Product, in yellow, has fragrance, crude protein 54.3%, little peptide content 26.9%, and viable count reaches 8*10^7/g, and oxidation resistance promotes 45%.
Claims (9)
1. a biological processing method for prebiotic cotton-seed polypeptide, is characterized in that, specifically comprise the steps:
(1) process of raw material: sterilizing or non-sterilization treatment are carried out to cottonseed flour and obtains the cottonseed flour after processing;
(2) preparation of withered grass gemma seed liquor: use bacillus subtilis (Bacillus subtilis) to adopt medium culture to obtain withered grass gemma seed liquor after 12 hours, the cell concentration of described withered grass gemma seed liquor reaches 10
9-10
10cfu/mL;
(3) preparation of mixed liquor is inoculated
Withered grass gemma seed liquor, enzyme preparation and the water step (2) prepared mix and obtain inoculating mixed liquor; Wherein the mass percent of withered grass gemma seed liquor is 17 ~ 25%, and the mass percent of enzyme preparation is 0.30 ~ 0.50%, and the mass percent of water is 75 ~ 83%;
(4) inoculation mixing: add in the cottonseed flour after the process of step (1) by inoculation mixed liquor prepared by step (3), obtains fermentation forward slip value thing after mixing; Material water quality between inoculation mixed liquor prepared by the cottonseed flour after the process of wherein step (1) and step (3) is than being 1:0.5 ~ 1:1.5;
(5) temperature controlled fermentation: the fermentation forward slip value thing of step (4) is inserted fermenting tank for fermentation, and thickness of feed layer controls at 10-30cm, temperature controlled fermentation 2 ~ 3 days; Prebiotic cotton-seed polypeptide fermentate is obtained after fermentation ends;
(6) pulverizing is dried: prebiotic cotton-seed polypeptide fermentate step (5) obtained is dried, pulverize, fill bag obtains wanted product.
2. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, it is characterized in that, the bacterial classification of described bacillus subtilis (Bacillus subtilis) sieves from wild environment, its strain number is BS13002, deposit number is CGMCC NO.8745, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
3. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, it is characterized in that, described culture medium contains the material of following mass percent: glucose 1.5%, peptone 1%, beef extract 1%, potassium chloride 0.3 ‰, calcium chloride 0.7 ‰, magnesium sulfate 0.2 ‰, the pH value of culture medium is 7.0.
4. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, is characterized in that, in described step (1), the condition of cottonseed flour being carried out to sterilizing is 100 ~ 120 DEG C, sterilization time 10 ~ 30 minutes.
5. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, it is characterized in that, in described step (4), the material water quality between inoculation mixed liquor prepared by the cottonseed flour after the process of described step (1) and step (3) compares 1:0.9.
6. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, is characterized in that, in described step (4), mixes rear adjustment pH to 7.2.
7. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, is characterized in that, in described step (5), fermentation temperature is 25 ~ 50 DEG C.
8. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, is characterized in that, in described step (6), bake out temperature is 60-80 DEG C.
9. the biological processing method of a kind of prebiotic cotton-seed polypeptide as claimed in claim 1, is characterized in that, described enzyme preparation is one or more the mixing in acid protease, neutral proteinase, alkali protease, flavor protease.
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Cited By (5)
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| CN105886423A (en) * | 2016-01-20 | 2016-08-24 | 江苏盐城源耀生物科技有限公司 | Preparation method of biofermentation rice bran meal |
| CN110150451A (en) * | 2017-11-30 | 2019-08-23 | 浙江省农业科学院 | A kind of method that bacterium enzyme collaboration glycolysis produces the low gossypol high quality cotton benevolence of full-cream high energy |
| CN110833115A (en) * | 2019-11-21 | 2020-02-25 | 南京胜昔实业有限公司 | Comprehensive utilization method of cotton production by-products by using biochemical fulvic acid as main product |
| CN111296679A (en) * | 2020-04-24 | 2020-06-19 | 苏州昆蓝生物科技有限公司 | Fermented feed for laying hens and preparation method thereof |
| CN112219947A (en) * | 2020-09-29 | 2021-01-15 | 新疆希普生物科技股份有限公司 | Composition containing cottonseed protein powder and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110833115A (en) * | 2019-11-21 | 2020-02-25 | 南京胜昔实业有限公司 | Comprehensive utilization method of cotton production by-products by using biochemical fulvic acid as main product |
| CN111296679A (en) * | 2020-04-24 | 2020-06-19 | 苏州昆蓝生物科技有限公司 | Fermented feed for laying hens and preparation method thereof |
| CN112219947A (en) * | 2020-09-29 | 2021-01-15 | 新疆希普生物科技股份有限公司 | Composition containing cottonseed protein powder and preparation method thereof |
| CN112219947B (en) * | 2020-09-29 | 2023-06-06 | 新疆希普生物科技股份有限公司 | Composition containing cottonseed protein powder and preparation method thereof |
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Application publication date: 20150304 |