CN112219947B - Composition containing cottonseed protein powder and preparation method thereof - Google Patents
Composition containing cottonseed protein powder and preparation method thereof Download PDFInfo
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- CN112219947B CN112219947B CN202011057605.6A CN202011057605A CN112219947B CN 112219947 B CN112219947 B CN 112219947B CN 202011057605 A CN202011057605 A CN 202011057605A CN 112219947 B CN112219947 B CN 112219947B
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- 239000000843 powder Substances 0.000 title claims abstract description 59
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 54
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 54
- 235000012343 cottonseed oil Nutrition 0.000 title claims abstract description 50
- 239000000203 mixture Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 91
- 240000008042 Zea mays Species 0.000 claims abstract description 34
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 34
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 34
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- 150000001413 amino acids Chemical class 0.000 claims abstract description 27
- CFWRDBDJAOHXSH-SECBINFHSA-N 2-azaniumylethyl [(2r)-2,3-diacetyloxypropyl] phosphate Chemical compound CC(=O)OC[C@@H](OC(C)=O)COP(O)(=O)OCCN CFWRDBDJAOHXSH-SECBINFHSA-N 0.000 claims abstract description 26
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 19
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims abstract description 18
- 235000019743 Choline chloride Nutrition 0.000 claims abstract description 18
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims abstract description 18
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- 150000001875 compounds Chemical class 0.000 claims abstract description 13
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- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 10
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
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- 239000002131 composite material Substances 0.000 claims description 18
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- 239000002245 particle Substances 0.000 claims description 13
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- 239000004472 Lysine Substances 0.000 claims description 9
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- 244000063299 Bacillus subtilis Species 0.000 claims description 8
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- 240000006024 Lactobacillus plantarum Species 0.000 claims description 8
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- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 8
- 229930182817 methionine Natural products 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000004475 Arginine Substances 0.000 claims description 7
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 7
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims description 7
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 7
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 7
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 7
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 7
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 7
- 229960003067 cystine Drugs 0.000 claims description 7
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- 238000004806 packaging method and process Methods 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 235000011148 calcium chloride Nutrition 0.000 claims description 6
- 229960002713 calcium chloride Drugs 0.000 claims 1
- 229940095079 dicalcium phosphate anhydrous Drugs 0.000 claims 1
- 229940111688 monobasic potassium phosphate Drugs 0.000 claims 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims 1
- 229960002668 sodium chloride Drugs 0.000 claims 1
- 238000010298 pulverizing process Methods 0.000 abstract description 12
- 239000003963 antioxidant agent Substances 0.000 abstract description 5
- 230000003078 antioxidant effect Effects 0.000 abstract description 5
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 235000011187 glycerol Nutrition 0.000 description 27
- 230000000052 comparative effect Effects 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 235000019687 Lamb Nutrition 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
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- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
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- 238000012935 Averaging Methods 0.000 description 1
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- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 description 1
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- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
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- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
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- 229920002678 cellulose Polymers 0.000 description 1
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- 210000004731 jugular vein Anatomy 0.000 description 1
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- -1 yikang XP Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Biomedical Technology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of biology, and particularly relates to a composition containing cottonseed protein powder and a preparation method thereof. The composition containing the cottonseed protein powder comprises the following components in parts by mass: 25 to 45 parts of cottonseed protein powder, 30 to 40 parts of corn, 3 to 8 parts of wheat bran, 0.3 to 3.8 parts of glycerol, 1.5 to 5 parts of cephalin, 0.2 to 1 part of choline chloride, 0.1 to 0.8 part of compound probiotics, 1 to 3 parts of glucose, 1 to 2 parts of lactose, 0.5 to 2.5 parts of amino acid and 0.5 to 2.5 parts of inorganic salt. The composition containing the cottonseed protein powder greatly reduces the pulverization rate, improves the content of T-SOD antioxidant enzyme and reduces the content of MDA.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a composition containing cottonseed protein powder and a preparation method thereof.
Background
Cottonseed is a by-product of cotton processing and mainly consists of two parts, namely, a shell and a seed kernel, wherein the main components of the shell are cellulose and lignin, and the total weight of the cottonseed accounts for about 30%, and the seed kernel is the main component of the cottonseed, accounts for about 60% of the total weight of the cottonseed, and comprises grease, protein, sugar, crude fiber and the like. In China, most of cotton seeds are used for producing vegetable oil, and researches show that: the byproduct cottonseed cake after oil extraction contains 38-45% of protein, and the content of other amino acids is higher except for the content of lysine from the aspect of the composition of amino acids, so that the overall nutrition is higher. However, in practical applications, the high gossypol content of cottonseed meal results in low nutrient digestibility of animals.
In order to solve the technical problems, the prior art mainly adopts a liquid-solid three-phase extraction method, a one-step method, a membrane-enhanced extraction method and a fermentation method to obtain cotton seed protein powder containing lower gossypol so as to be applied to animal feed. For example, chinese patent CN105851562a discloses a compound feed for improving the carcass quality of fattening pigs under low nitrogen emission ration, which is prepared by compounding corn, soybean meal, cottonseed protein, wheat bran, rice bran, soybean oil, calcium hydrophosphate, calcium carbonate, lysine sulfate, threonine, methionine, tryptophan, sodium chloride, betaine, sodium bicarbonate, calcium propionate, ethoxyquinoline, microecological preparation and compound premix feed for fattening pigs, and can have influence on the growth performance of pigs, the pork quality, lipid in blood and protein metabolism.
At present, most of cottonseed protein powder is fed in the form of powder, and production tests for many years prove that compared with the powder, the granular material has the advantages of uniform nutrition, good palatability and high ingestion rate, and can inhibit toxin through granulation, and dust can be reduced during feeding, so that the influence on animal respiratory diseases is reduced, and therefore, the cottonseed protein powder with higher nutrition components is made into granular material with very important significance. For example, chinese patent CN103461727A discloses a lamb concentrate supplement granule and a preparation method thereof, and the lamb concentrate supplement granule is prepared from corn, molasses, bean pulp, corn germ meal, lactose, yikang XP, antioxidant, rice bran, DDGS, cottonseed protein powder, stone powder, calcium hydrophosphate, baking soda, salt, feeding microecological preparation, acidulant, rumen bypass fat and premix special for mutton sheep by a specific process. However, cottonseed protein meal is rarely manufactured into swine granules which require proper hardness during use and should minimize pulverization rates.
Disclosure of Invention
The invention aims to provide a composition containing cottonseed protein powder and a preparation method thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the composition containing the cottonseed protein powder comprises the following components in parts by weight: 25 to 45 parts of cottonseed protein powder, 30 to 40 parts of corn, 3 to 8 parts of wheat bran, 0.3 to 3.8 parts of glycerol, 1.5 to 5 parts of cephalin, 0.2 to 1 part of choline chloride, 0.1 to 0.8 part of compound probiotics, 1 to 3 parts of glucose, 1 to 2 parts of lactose, 0.5 to 2.5 parts of amino acid and 0.5 to 2.5 parts of inorganic salt.
Further, the mass ratio of the glycerol to the cephalin is (1.5-3): 1.
further, the composite material comprises the following components in parts by weight: 30 parts of cottonseed protein powder, 30 parts of corn, 5 parts of wheat bran, 3.5 parts of glycerol, 1.75 parts of cephalin, 0.3 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1 part of lactose, 1.2 parts of amino acid and 1.2 parts of inorganic salt.
Further, the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps (2-4): 1 mass ratio.
Further, the amino acid includes at least one of lysine, methionine, cystine, tryptophan, arginine, and leucine.
Further, the inorganic salt comprises at least one of sodium chloride, calcium chloride, potassium dihydrogen phosphate and calcium hydrogen phosphate.
The invention provides a preparation method of any one of the cotton seed protein powder-containing compositions, which is characterized by comprising the following steps:
s1) crushing corn, and sieving to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, mixing the mixture with cotton seed protein powder and glycerol after steam tempering, introducing the mixture into a granulator, extruding and granulating, and mixing the mixture with composite probiotics to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
In the present invention, cottonseed protein powder has viscoelasticity and plasticity, and can increase compactibility after being mixed with glycerol, but it was found that even though the pulverization rate of particles obtained by mixing cottonseed protein powder with glycerol is still relatively high, the reason may be that the composition is affected by the addition of other components, and after experiments, it was found that the pulverization rate of particles can be reduced after adding a certain amount of cephalin to the composition, and it is presumed that the uniformity of mixing can be improved by adding cephalin to other components before mixing with cottonseed protein powder.
Further, the tempering temperature in the step S2 is 95-105 ℃ and the time is 2-5 min.
Further, the adding mode of the glycerol and the compound probiotics in the step S2 is spraying.
Further, the diameter of the granulating ring die of the granulator in the step S2 is 3.5-6.0 mm.
Compared with the prior art, the invention has the following beneficial effects:
(1) The cottonseed protein powder and other components are prepared into particles, and experiments show that the particles have low moisture content and moderate hardness, have low pulverization rates of 30s, 60s, 90s and 120s, can reduce dust and reduce the influence on animal respiratory diseases.
(2) Experiments show that the composition containing the cottonseed protein powder has high content of T-SOD and low content of MDA, can improve the oxidation stress resistance of piglets and promote the weight gain of the piglets.
Detailed Description
The above-described aspects of the present invention will be described in further detail with reference to the following embodiments. It should not be construed that the scope of the above subject matter of the present invention is limited to the following examples.
In the invention, the product name of the cottonseed protein powder is 'plejian', and the crude protein is: not less than 50.0%, calcium: more than or equal to 0.24 percent, the moisture is less than or equal to 12.0 percent, the total phosphorus is more than or equal to 0.90 percent, and the crude fiber is: less than or equal to 10.0 percent, salt: more than or equal to 0.10 percent, coarse ash content less than or equal to 9.0 percent, and rice branAmino acid is more than or equal to 2.00%, and free gossypol content: less than or equal to 400ppm, total number of viable probiotics: not less than 1X 10 8 cfu/g, available from Xinjiang Hippy biotechnology Co., ltd.
Lactobacillus plantarum and Bacillus subtilis are both purchased from the Guangdong province microorganism strain collection, and GIM numbers are 1.1516 and 1.131, respectively.
Example 1
The composition containing the cottonseed protein powder comprises the following components in parts by weight: 35 parts of cottonseed protein powder, 30 parts of corn, 5 parts of wheat bran, 3.5 parts of glycerol, 1.75 parts of cephalin, 0.5 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1 part of lactose, 1 part of amino acid and 1 part of inorganic salt.
Wherein,,
the mass ratio of glycerin to cephalin is 2:1, a step of;
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of 2:1 mass ratio;
amino acids include lysine, methionine, cystine, tryptophan, arginine, and leucine;
inorganic salts include sodium chloride, calcium chloride, potassium dihydrogen phosphate, and calcium hydrogen phosphate.
The preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving with a 80-mesh sieve to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, performing steam tempering, wherein the tempering temperature is 100 ℃, the time is 3min, mixing with cotton seed protein powder and glycerol, adding glycerol in a spraying manner, introducing into a granulator, granulating with the caliber of a circular mould of 3.8mm, extruding and granulating, mixing with composite probiotics, and adding the composite probiotics in a spraying manner to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
Example 2
The composition containing the cottonseed protein powder comprises the following components in parts by weight: 30 parts of cottonseed protein powder, 30 parts of corn, 5 parts of wheat bran, 3.5 parts of glycerol, 1.75 parts of cephalin, 0.3 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1 part of lactose, 1.2 parts of amino acid and 1.2 parts of inorganic salt.
Wherein,,
the mass ratio of glycerin to cephalin is 2:1, a step of;
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of 3:1 mass ratio;
amino acids include lysine, methionine, cystine, tryptophan, arginine, and leucine;
the inorganic salt comprises sodium chloride, potassium dihydrogen phosphate and calcium hydrogen phosphate.
The preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving with a 80-mesh sieve to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, performing steam tempering, wherein the tempering temperature is 100 ℃, the time is 3min, mixing with cotton seed protein powder and glycerol, adding glycerol in a spraying manner, introducing into a granulator, granulating with the caliber of a circular mould of 4.0mm, extruding and granulating, mixing with composite probiotics, and adding the composite probiotics in a spraying manner to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
Example 3
The composition containing the cottonseed protein powder comprises the following components in parts by weight: 30 parts of cottonseed protein powder, 35 parts of corn, 6 parts of wheat bran, 3.8 parts of glycerol, 1.52 parts of cephalin, 0.45 part of choline chloride, 0.6 part of compound probiotics, 1 part of glucose, 2 parts of lactose, 1.5 parts of amino acid and 1 part of inorganic salt.
Wherein,,
the mass ratio of glycerin to cephalin is 2.5:1, a step of;
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of 3:1 mass ratio;
amino acids include lysine, methionine, cystine, tryptophan, arginine, and leucine;
inorganic salts include sodium chloride, calcium chloride, potassium dihydrogen phosphate, and calcium hydrogen phosphate.
The preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving with a 80-mesh sieve to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, performing steam tempering, wherein the tempering temperature is 95 ℃, the time is 4min, mixing the wheat bran, cephalin, choline chloride, saccharides, amino acids and the corn flour with cotton seed protein powder and glycerol, spraying the mixture, introducing a granulator, granulating into a circular mould with the caliber of 4.5mm, extruding and granulating, mixing the granules with composite probiotics, and spraying the mixture to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
Example 4
The composition containing the cottonseed protein powder comprises the following components in parts by weight: 35 parts of cottonseed protein powder, 35 parts of corn, 6 parts of wheat bran, 3.5 parts of glycerol, 1.4 parts of cephalin, 0.5 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1.5 parts of lactose, 1.3 parts of amino acid and 0.8 part of inorganic salt.
Wherein,,
the mass ratio of glycerin to cephalin is 1.5:1, a step of;
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of 3:1 mass ratio;
amino acids include lysine, methionine, cystine, tryptophan, arginine, and leucine;
inorganic salts include sodium chloride, calcium chloride, potassium dihydrogen phosphate, and calcium hydrogen phosphate.
The preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving with a 80-mesh sieve to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, performing steam tempering, wherein the tempering temperature is 102 ℃, the time is 2min, mixing with cotton seed protein powder and glycerol, adding glycerol in a spraying manner, introducing into a granulator, granulating with the caliber of a circular mould of 3.5mm, extruding and granulating, mixing with composite probiotics, and adding the composite probiotics in the spraying manner to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
Comparative example 1
The composition containing the cottonseed protein powder comprises the following components in parts by weight: 35 parts of cottonseed protein powder, 30 parts of corn, 5 parts of wheat bran, 3.5 parts of glycerol, 0.5 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1 part of lactose, 1 part of amino acid and 1 part of inorganic salt.
Wherein,,
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of 2:1 mass ratio;
amino acids include lysine, methionine, cystine, tryptophan, arginine, and leucine;
inorganic salts include sodium chloride, calcium chloride, potassium dihydrogen phosphate, and calcium hydrogen phosphate.
The preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving with a 80-mesh sieve to obtain corn flour;
s2) uniformly mixing wheat bran, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, performing steam tempering, wherein the tempering temperature is 100 ℃, the time is 3min, mixing the wheat bran, choline chloride, saccharides, amino acids and inorganic salts with cotton seed protein powder and glycerol, spraying the mixture, introducing a granulator, granulating into a circular mould with the caliber of 3.8mm, extruding and granulating, mixing the granules with composite probiotics, and spraying the mixture to obtain granules;
and S3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2.
Similar to example 1, except that cephalin was not added.
Comparative example 2
Similar to example 1, the difference is that the mass ratio of glycerol to cephalin is 1:1.
comparative example 3
Similar to example 1, except that glycerol was directly mixed with cottonseed protein powder in step S2, and was not added by spraying.
Comparative example 4
Similar to example 1, except that cottonseed protein meal was replaced with soybean meal.
Experiment one, performance measurement
1.1 moisture determination
The experimental method comprises the following steps: the example/comparative example was weighed to give W0, g, and then dried in an oven at 103℃for 4 hours to give W1, g, the difference between W0 and W1 being the moisture, g.
1.2 hardness measurement
The experimental method comprises the following steps: the measurement was performed using a durometer.
1.3 determination of the pulverization Rate
The experimental method comprises the following steps: 100g of the example/comparative example was taken, placed in a particle durability tester, the timing buttons were turned on for 30s, 60s, 90s and 120s, and after the completion, the residual particles were weighed.
The calculation formula is as follows:
TABLE 1
As can be seen from Table 1, examples 1 to 4 were low in moisture content, moderate in hardness, and low in pulverization rate at 30s, 60s, 90s and 120 s.
Comparative example 1 has a higher pulverization rate at 30s, 60s, 90s and 120s than example 1, whereas comparative example 2 has a higher pulverization rate than example 1, but has a lower pulverization rate than comparative example 1, indicating that addition of cephalin helps to reduce pulverization rate, and comparative example 3 adjusts the addition of glycerin to result in an increase in pulverization rate.
Experiment two, influence on piglet growth
2.1 body weight measurement
The experimental method comprises the following steps: collecting 9 weaned piglets, dividing into 3 groups, weighing, and averaging W 0 Kg, the composition containing cottonseed protein powder of example 1/comparative example 4 was fed at fixed time and fixed amount, and fed for 21 days, weighed on day 22, and averaged for W 1 ，kg。
2.2 antioxidant function assay
The experimental method comprises the following steps: on day 22, the jugular vein of the pig is sampled, kept stand at room temperature for 30min, refrigerated at 4 ℃ for 30min, centrifuged at 3500rpm for 10min, serum is separated, and then detected by using a T-SOD activity detection kit and an MDA content detection kit (Nanjing built bioengineering institute).
TABLE 2
In example 1, the content of T-SOD antioxidant enzyme is increased, the content of MDA is low, and MDA reflects the lipid peroxidation degree of tissue cells, so that the piglet in example 1 has better antioxidant stress capability.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.
Claims (3)
1. The composition containing the cottonseed protein powder is characterized by comprising the following components in parts by mass: 30 parts of cottonseed protein powder, 30 parts of corn, 5 parts of wheat bran, 3.5 parts of glycerol, 1.75 parts of cephalin, 0.3 part of choline chloride, 0.5 part of compound probiotics, 2 parts of glucose, 1 part of lactose, 1.2 parts of amino acid and 1.2 parts of inorganic salt;
the composite probiotics are prepared from lactobacillus plantarum and bacillus subtilis according to the following steps of (2-4): 1 mass ratio;
the mass ratio of the glycerol to the cephalin is (1.5-3): 1, a step of;
the preparation method of the composition containing the cottonseed protein powder comprises the following steps:
s1) crushing corn, and sieving to obtain corn flour;
s2) uniformly mixing wheat bran, cephalin, choline chloride, saccharides, amino acids, inorganic salts and the corn flour in the step S1, mixing the mixture with cotton seed protein powder and glycerol after steam tempering, introducing the mixture into a granulator, extruding and granulating, and mixing the mixture with composite probiotics to obtain granules;
s3) sequentially drying, cooling, sieving, removing powder and packaging the particles obtained in the step S2; the step S2 is to add glycerol and the compound probiotics in a spraying mode;
the tempering temperature in the step S2 is 95-105 ℃ and the time is 2-5 min.
2. The composition of claim 1, wherein the amino acid comprises at least one of lysine, methionine, cystine, tryptophan, arginine, and leucine.
3. The composition of claim 1, wherein the inorganic salt comprises at least one of sodium chloride, calcium chloride, monobasic potassium phosphate, dibasic calcium phosphate.
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