CN108634123A - A kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal and its preparation - Google Patents
A kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal and its preparation Download PDFInfo
- Publication number
- CN108634123A CN108634123A CN201810450817.7A CN201810450817A CN108634123A CN 108634123 A CN108634123 A CN 108634123A CN 201810450817 A CN201810450817 A CN 201810450817A CN 108634123 A CN108634123 A CN 108634123A
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- China
- Prior art keywords
- feed
- cotton dregs
- dregs
- high protein
- layer chicken
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 85
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims description 69
- 108090000623 proteins and genes Proteins 0.000 title claims description 69
- 235000019764 Soybean Meal Nutrition 0.000 title claims description 20
- 239000004455 soybean meal Substances 0.000 title claims description 20
- 238000006467 substitution reaction Methods 0.000 title claims description 20
- 238000002360 preparation method Methods 0.000 title description 5
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 claims abstract description 36
- 108010059345 keratinase Proteins 0.000 claims abstract description 35
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 31
- 239000000787 lecithin Substances 0.000 claims abstract description 31
- 229940067606 lecithin Drugs 0.000 claims abstract description 31
- 235000010445 lecithin Nutrition 0.000 claims abstract description 31
- 235000013312 flour Nutrition 0.000 claims abstract description 29
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims abstract description 19
- 235000019700 dicalcium phosphate Nutrition 0.000 claims abstract description 19
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229930000755 gossypol Natural products 0.000 claims abstract description 18
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- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 13
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 13
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 10
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 10
- 239000010457 zeolite Substances 0.000 claims abstract description 10
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000004472 Lysine Substances 0.000 claims abstract description 9
- 240000008042 Zea mays Species 0.000 claims abstract description 9
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- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 8
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims description 4
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- 230000004044 response Effects 0.000 description 18
- 235000013601 eggs Nutrition 0.000 description 17
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 12
- 239000011575 calcium Substances 0.000 description 12
- 229910052791 calcium Inorganic materials 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 238000012360 testing method Methods 0.000 description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 8
- 230000029087 digestion Effects 0.000 description 8
- 229910052698 phosphorus Inorganic materials 0.000 description 8
- 239000011574 phosphorus Substances 0.000 description 8
- 102000057297 Pepsin A Human genes 0.000 description 7
- 108090000284 Pepsin A Proteins 0.000 description 7
- 229940111202 pepsin Drugs 0.000 description 7
- 239000000523 sample Substances 0.000 description 6
- 238000005457 optimization Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 108010019160 Pancreatin Proteins 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 229940055695 pancreatin Drugs 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 102000006395 Globulins Human genes 0.000 description 3
- 108010044091 Globulins Proteins 0.000 description 3
- 235000012343 cottonseed oil Nutrition 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
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- 239000000463 material Substances 0.000 description 3
- 235000013594 poultry meat Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
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- 102000009027 Albumins Human genes 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 208000005374 Poisoning Diseases 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
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- 238000002474 experimental method Methods 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
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- 210000004885 white matter Anatomy 0.000 description 2
- WDCYWAQPCXBPJA-UHFFFAOYSA-N 1,3-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC([N+]([O-])=O)=C1 WDCYWAQPCXBPJA-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 1
- 108010082126 Alanine transaminase Proteins 0.000 description 1
- NTZRDKVFLPLTPU-UHFFFAOYSA-N CC[Na] Chemical compound CC[Na] NTZRDKVFLPLTPU-UHFFFAOYSA-N 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 206010018092 Generalised oedema Diseases 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 206010037423 Pulmonary oedema Diseases 0.000 description 1
- 235000019779 Rapeseed Meal Nutrition 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
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- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 201000001505 hemoglobinuria Diseases 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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- 239000004456 rapeseed meal Substances 0.000 description 1
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- 229930003231 vitamin Natural products 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses layer chicken feed basal feeds to be by weight percentage：Dregs of beans 12.6%, cotton dregs 10%, mountain flour 8%, calcium monohydrogen phosphate 1.8%, premix 1%, vegetable oil 1%, salt 0.3%, zeolite powder 2.4%, lysine 0.1%, ferrous sulfate 0.002%, remaining is corn；It is corresponded to per kg cotton dregs in basal feed and keratinase 1g and lecithin 20g is added.The present invention makes gossypol toxicity weaken by the adjusting of formula, it reduces to injuries such as laying hen gastrointestinal mucosas, also the nutriment in gossypol albumen is utilized to greatest extent, reduce because nutriment is lost in caused by fermentation etc., improve digestibility of the poultry to feed, save feed cost, the cotton dregs of addition 10% can reduce the death and culling rate of laying hen, improve fertility rate and hatchability, it can also make every strong female 0.03 yuan young of hatching egg cost reduction in economic benefit, there is wide applicability in layer chicken feed field.
Description
Technical field
The invention belongs to feed formula technology fields, and in particular to a kind of laying hen feeding of high protein cotton dregs Substitution for Soybean Meal
Material formula.
Background technology
With aquaculture industry of China scale, intensive development, the demand to protein feed resource has been for not answering
It asks or even wretched insufficiency.All the time, fish meal, dregs of beans, rapeseed meal, meat meal tankage etc. are common protein feed sources, however
These feeds largely rely on import, and higher price, and safety is relatively low, seriously constrains the sustainable development of aquaculture industry of China
Exhibition.Therefore, seek more suitably protein resource and have become a urgent problem to be solved.
Cottonseed Meal is the byproduct of oils preparation industry, is a kind of important protein feed in production cotton countries and regions.China
Cottonseed Meal is resourceful, is a kind of excellent protein feed resources.Tryptophan, methionine are abundant in Cottonseed Meal, vitamin content
Also higher.Cotton dregs thick protein made of the cotton benevolence to shell completely is up to 45%, even as high as 50% or more.The egg of cotton cake dregs
White matter is mainly made of 17 kinds of amino acid.
But anti-nutritional factors gossypol and cyclopropenoid fatty acids present in cotton dregs, cotton dregs are limited in animal and fowl fodder
In application, wherein what is played a major role is the virose gossypol of tool, in cotton dregs the presence of gossypol bring asking for two aspects
Topic：First, the combination between gossypol and protein reduces the utilization rate of protein, to reduce the product of feed protein
Matter；Second is that high-level gossypol exists and the interior gossypol accumulation of tissue causes the unfavorable physiological reaction of body.Livestock and poultry gossypol poisoning shows
For anorexia, weight loss, anasarca is weak, expiratory dyspnea, hemoglobinuria, hemorrhagic enteritis, pulmonary edema, liver
With myocardial deformation necrosis.Weaken the method for gossypol toxicity although having ferment by cotton dregs in the prior art, process is multiple
Miscellaneous, cost is higher, and wastes serious.
Invention content
It is rich in nutriment for cotton dregs in the prior art, but the poisonous and harmful element contained by it leads to cotton dregs egg
In vain without processing be difficult to directly utilize, causing to process complicated cost again by the method for fermentation process increases, and can also be formed
The technical problems such as waste material waste residue, the present invention provides a kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal and its preparation sides
Method makes gossypol toxicity weaken, reduces to injuries such as laying hen gastrointestinal mucosas, also to greatest extent by the adjusting of formula
Using the nutriment in gossypol albumen, reduce because nutriment is lost in caused by fermentation etc., it is provided by the invention to lay eggs
Chicken feed additive improves digestibility of the poultry to feed, saves feed cost.
The present invention provides a kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal, the layer chicken feed basis is raised
Material be by weight percentage：It is dregs of beans 12.6%, cotton dregs 10%, mountain flour 7.5%-8.5%, calcium monohydrogen phosphate 1.5%-2%, pre-
Batch mixing 1%, vegetable oil 1%, salt 0.3%, zeolite powder 2%-2.6%, lysine 0.1%, ferrous sulfate 0.002%, remaining
For corn.
It is corresponded to per kg cotton dregs in above-mentioned basal feed and feed addictive keratinase 0.8g-1.2g is added.
It is corresponded to per kg cotton dregs in above-mentioned basal feed and feed addictive lecithin 15g-25g is added.
In the present invention, the crude protein content of high protein cotton dregs is 55%, free gossypol 160mg/kg.
In the present invention, prolease activity >=150000U/g of feed addictive keratinase.
In the present invention, layer chicken feed basal feed is by weight percentage：Dregs of beans 12.6%, cotton dregs 10%, mountain flour
8%, calcium monohydrogen phosphate 1.8%, premix 1%, vegetable oil 1%, salt 0.3%, zeolite powder 2.4%, lysine 0.1%, sulfuric acid
Ferrous iron 0.002%, remaining is corn.
It is corresponded to per kg cotton dregs in the present invention, in basal feed and feed addictive keratinase 1g is added.
It is corresponded to per kg cotton dregs in the present invention, in basal feed and feed addictive lecithin 20g is added.
In the present invention, high protein cotton dregs are the cotton dregs by crushing and crossing 40-60 mesh sieve.
In the present invention, premix uses commercially available common laying hen premix.
Beneficial effects of the present invention:
A kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal provided by the invention and its preparation, pass through the tune of formula
Section is reduced to injuries such as laying hen gastrointestinal mucosas, also to greatest extent using in gossypol albumen so that gossypol toxicity weakens
Nutriment reduces because nutriment is lost in caused by fermentation etc., and layer chicken feed formula provided by the invention improves
Poultry saves feed cost to the digestibility of feed, adds 10% cotton dregs and can reduce the death and culling rate of laying hen, improves rate of fertilization
And hatching rate, and laying rate is not influenced, every strong female 0.03 yuan young of hatching egg cost reduction can also be made in economic benefit,
There is wide applicability in layer chicken feed field.
Description of the drawings
Fig. 1 is the dry matter digestibility and protein digestibility that the different pepsin concns of the present invention correspond to high protein cotton dregs
Rate；
Fig. 2 is the dry matter digestibility and protein digestibility that the different pancreas enzyme concentrations of the present invention correspond to high protein cotton dregs；
Fig. 3 is shown as keratinase dosage and lecithin dosage to egg cost response surface curve graph.
Fig. 4 is shown as keratinase dosage and mountain flour dosage to egg cost response surface curve graph.
Fig. 5 is shown as keratinase dosage and calcium monohydrogen phosphate additive amount to egg cost response surface curve graph.
Fig. 6 is shown as lecithin dosage and mountain flour dosage to egg cost response surface curve graph.
Fig. 7 is shown as lecithin dosage and calcium monohydrogen phosphate additive amount to egg cost response surface curve graph.
Fig. 8 is shown as mountain flour dosage and calcium monohydrogen phosphate additive amount to egg cost response surface curve graph.
Fig. 9 is shown as keratinase dosage and lecithin dosage to dry matter digestibility response surface curve graph.
Figure 10 is shown as keratinase dosage and mountain flour dosage to dry matter digestibility response surface curve graph.
Figure 11 is shown as keratinase dosage and calcium monohydrogen phosphate additive amount to dry matter digestibility response surface curve graph.
Figure 12 is shown as lecithin dosage and mountain flour dosage to dry matter digestibility response surface curve graph.
Figure 13 is shown as lecithin dosage and calcium monohydrogen phosphate additive amount to dry matter digestibility response surface curve graph.
Figure 14 is shown as mountain flour dosage and calcium monohydrogen phosphate additive amount to dry matter digestibility response surface curve graph.
The corresponding dry matter digestibility of the different high protein cotton dregs additive amounts of Figure 15 present invention and protein digestibility；
The corresponding dry matter digestibility of the different keratinase additive amounts of Figure 16 present invention and protein digestibility；
The corresponding dry matter digestibility of the different calcium contents of Figure 17 present invention and protein digestibility；
The corresponding dry matter digestibility of the different phosphorus contents of Figure 18 present invention and protein digestibility；
The corresponding dry matter digestibility of the different accession capacity of Lecithin of Figure 19 present invention and protein digestibility；
Specific implementation mode
1-19 and embodiment are described in further detail the specific implementation mode of the present invention below in conjunction with the accompanying drawings, but this
The method of invention is not limited to following embodiments.
Embodiment one：High protein cotton dregs layer chicken feed of the present invention
The present invention provides a kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal, the layer chicken feed basis is raised
Material be by weight percentage：Dregs of beans 12.6%, mountain flour 7.5%, calcium monohydrogen phosphate 1.5%, premix 1%, is planted at cotton dregs 10%
Object oil 1%, salt 0.3%, zeolite powder 2%, lysine 0.1%, ferrous sulfate 0.002%, remaining is corn.
It is corresponded to per kg cotton dregs in above-mentioned basal feed and feed addictive keratinase 0.8g is added.
It is corresponded to per kg cotton dregs in above-mentioned basal feed and feed addictive lecithin 15g is added.
In the present invention, the crude protein content of high protein cotton dregs is 55%, free gossypol 160mg/kg.
In the present invention, prolease activity >=150000U/g of feed addictive keratinase.
In the present invention, premix uses commercially available common laying hen premix.
Embodiment two：High protein cotton dregs layer chicken feed of the present invention
Layer chicken feed basal feed is by weight percentage：Dregs of beans 12.6%, cotton dregs 10%, mountain flour 8%, phosphoric acid
Hydrogen calcium 1.8%, premix 1%, vegetable oil 1%, salt 0.3%, zeolite powder 2.4%, lysine 0.1%, ferrous sulfate
0.002%, remaining is corn.
It is corresponded to per kg cotton dregs in the present invention, in basal feed and feed addictive keratinase 1g is added.
It is corresponded to per kg cotton dregs in the present invention, in basal feed and feed addictive lecithin 20g is added.
Embodiment three：High protein cotton dregs layer chicken feed of the present invention
Layer chicken feed basal feed is by weight percentage：Dregs of beans 12.6%, cotton dregs 10%, mountain flour 8.5%, phosphorus
Sour hydrogen calcium 2%, premix 1%, vegetable oil 1%, salt 0.3%, zeolite powder 2.6%, lysine 0.1%, ferrous sulfate
0.002%, remaining is corn.
It is corresponded to per kg cotton dregs in the present invention, in above-mentioned basal feed and feed addictive keratinase 1.2g is added.
It is corresponded to per kg cotton dregs in the present invention, in above-mentioned basal feed and feed addictive lecithin 25g is added.
Example IV：The measurement of layer chicken feed Vitro Digestibility of the present invention
The one pancreatin two-step method of pepsin of use measures the Vitro Digestibility of something in feed, and appropriately adjusts.
Each sample makees 3 Duplicate Samples.
Test data carries out one-way analysis of variance (ANOVA) using SPSS16.0 softwares commonly used in the art and handles, and adopts
The significance of difference (the P of average is analyzed with Duncan multiple comparison graphs<0.05).Numerical value is indicated with average scholar's standard deviation.
1. the selection of the most suitable enzyme dosage of In vitro digestion trial
The selection of 1.1 pepsin optimum doses
Select pepsin concn for 40,50,60,70,80mg/ml, it is real to carry out digestion using high protein cotton dregs as substrate
It tests, acquired results are referring to shown in attached drawing 1.According to different pepsin concns to the dry matter digestibility and egg of high protein cotton dregs
The influence of white matter digestibility determines that most suitable pepsin concn is 70mg/ml.
The selection of 1.2 pancreatin optimal doses
After high protein cotton dregs are with the pepsin digestion 75min of a concentration of 70mg/ml, then be separately added into containing pancreatin 30,
40, the ethyl sodium buffer solution of 50,60,70mg continues to digest 180min.Its dry matter and protein digestibility are measured,
As a result referring to shown in attached drawing 2.Determine that the optimum dose of pancreatin is 50mg.
Embodiment five：Layer chicken feed toxicological test of the present invention
1. feed, laying hen
Feed using the present invention, all with the feed of dregs of beans and is not added with keratin at commercially available conventional laying hen mixed feed
Enzyme and 40 week old Roman egghen of lecithin forage feed 400.
2. testing program
400 chickens are divided into four groups, the commercially available conventional laying hen mixed feed of 1 group of feeding, 2 groups use the embodiment of the present invention
The feed of two feed formulas, 3 groups using being not added with keratinase and lecithin feed, 4 groups of bean-dregs feeds, be not added with keratinase and
Lecithin feed other compositions and embodiment two are identical, and the cotton dregs in all embodiments of bean-dregs feed two are substituted with dregs of beans.Each group
After feed feeds 7d in advance, when the adjusted feeding of each group laying rate reaches all identical, then research and the record of every content are carried out.
Formal test research is 37d, and last ld each groups acquire the analysis that 7-8 chicken serum carries out biochemical indicator.Gpt activity
Measurement, with 2,4- dinitrobenzene trap methods；The double vena contracta methods of the measurement of total protein；The measurement electrophoresis of albumin and globulin
Detach development process.
There are 7 death using the chicken for being not added with keratinase and lecithin feed for 3 groups after the end of the test, other chickens are just
Often survival.
Table 1：Layer chicken feed toxicological test data
It is seen from the above data that the scheme for the cotton dregs Substitution for Soybean Meal that the present invention uses is to have saved great amount of cost,
It solves the problems, such as directly to use cotton dregs that the death rate of chicken is caused to rise, in terms of experimental data, glutamic-pyruvic transaminase of the invention is lived
1 group of property and control group, 4 groups of result are essentially identical, and difference is not notable.Illustrate that the liver of test group chicken does not have gossypol poisoning disease
Shape.And the chicken serum albumin of forage feed of the present invention and percentage contents, albumin and the globulin ratio of globulin
It is all almost the same with control group, or even slightly above control group.Illustrate that the Liver of Laying Hens of two groups of B, C is synthesizing albuminised function just
Often.
Embodiment six：The optimization of the layer chicken feed formula of high protein cotton dregs Substitution for Soybean Meal
Using Box-Behnken experimental designs, probes into keratinase dosage, lecithin dosage, mountain flour, calcium monohydrogen phosphate and add
The response surface mathematical model of dosage and egg cost, protein digestibility and dry matter digestibility, response surface experiments factor and level
Table is shown in Table 2, Box-bohnken experimental designs and the results are shown in Table 3.
Table 2：Response surface experiments factor level table
| Process conditions | Unit | Minimum value | Maximum value |
| Keratinase dosage | G/kg cotton dregs | 0.8 | 1.2 |
| Lecithin dosage | G/kg cotton dregs | 15 | 25 |
| Mountain flour | % | 7.5 | 8.5 |
| Calcium monohydrogen phosphate additive amount | % | 1.5 | 2 |
| Egg cost | Member/kg | 3.61 | 3.91 |
| Dry matter digestibility | % | 40 | 58 |
Table 3：Box-bohnken experimental design results
The experimental data of table 2 is fitted by Design Expert8.0.6, obtains keratinase dosage, lecithin
Fat dosage, mountain flour, calcium monohydrogen phosphate additive amount examine the response surface design figure of egg cost, protein digestibility and dry matter digestibility
The shape of be fitted response surface design is examined, analysis keratinase dosage, lecithin dosage, mountain flour, calcium monohydrogen phosphate additive amount are to egg
The influence of cost, protein digestibility and dry matter digestibility, each interactive response surface of factor is referring to attached drawing 3 in model
To attached drawing 14.
Box-Behnken assay optimizations go out the layer chicken feed formula of high protein cotton dregs Substitution for Soybean Meal, and optimum proportioning is
Dregs of beans 12.6%, cotton dregs 10%, mountain flour 8%, calcium monohydrogen phosphate 1.8%, premix 1%, vegetable oil 1%, salt 0.3%, zeolite
Powder 2.4%, lysine 0.1%, ferrous sulfate 0.002%, remaining is corn, corresponds to be added per kg cotton dregs in basal feed and raise
Feed additives keratinase 1g, additive lecithin 20g, at this moment the incidence of chicken is minimum, and the protein content of egg is best,
The cost of egg is also minimum to reach 3.61 yuan/kg, and dry matter digestibility is preferably also 58%.
Embodiment seven：The optimization of the layer chicken feed formula of high protein cotton dregs Substitution for Soybean Meal
Based on test feed basic components, according to equal nitrogen etc. can principle, be separately added into 0,50,100,150,
The high protein cotton dregs Substitution for Soybean Meal of 200g/kg is prepared a kind of with dregs of beans, the test feed that high protein cotton dregs are main protein sources.
The external digestion of different high protein cotton dregs Substitution for Soybean Meal mixed feeds is referring to shown in attached drawing 15.
As shown in Figure of description 15, with the increase of high protein cotton dregs replacement amount, dry matter digestibility is in first to increase to subtract afterwards
Trend, protein digestibility when replacement amount is smaller there are one first subtracting the process increased afterwards, later as the increase of replacement amount is in
The protein digestibility of downward trend, control group Feed Sample is significantly higher than processing group, and the additive amount of high protein cotton dregs is
When 100g/kg, the protein digestibility of Feed Sample is significantly higher than other processing groups.The result shows that high protein cotton dregs additive amount
For 100g/kg when, dry matter digestibility peaks, and protein digestibility is also higher, respectively 0.58,0.57, Zhi Housui
The increase of cotton dregs additive amount, Vitro Digestibility are remarkably decreased.
1. the determination of keratinase additive amount
On the feed base of the cotton dregs of high protein containing 100g/kg, addition 0.0%, 0.03%, 0.06%, 0.09%,
0.12% keratinase studies influence of the different keratinase additive amounts to mixed feed digestibility, to choose most suitable angle egg
White enzyme additive amount, optimizes layer chicken feed formula.Referring to the keratinase that attached drawing 16 is Different adding amount to feed external digestion
The influence of rate.
Shown in attached drawing 16, with the addition of keratinase, diet dry matter digestion takes the lead in reducing after increasing, and adds
The Vitro Digestibility of amount 0.09% is significantly higher.The variation of protein digestibility is similar to the variation of dry matter digestibility, presents
Going out the trend for first increasing and subtracting afterwards, the keratinase protein digestibility and dry matter digestibility for adding 0.09% reach maximum value, point
It Wei 0.61,0.584.
Consider influence of the keratinase Different adding amount to Vitro Digestibility, determines the requirement model of keratinase
It encloses for 0.08%-0.10%.
2. the determination of mountain flour Optimum Requirement
The main component of mountain flour is calcium carbonate, and 38% or more calcium content can cook the replenishers of calcium in feed.Add above-mentioned
On the basis of adding keratinase feed formula, 4%, 5.3%, 6.6%, 8%, 9.2% is added respectively, and increase zeolite powder and add
Dosage is to reach proportion balance.Study influence of the mountain flour to calcium digestibility and dry matter digestibility.As a result referring to 17 institute of attached drawing
Show.
Shown in attached drawing 17, with the increase of mountain flour additive amount, diet dry matter digestion takes the lead in subtracting after increasing, additive amount
8% sample Vitro Digestibility highest.The variation of calcium digestibility is similar to the variation of dry matter digestibility, and presentation first increases to be subtracted afterwards
Trend, the mountain flour for adding 8% reaches maximum value 37.5%, is significantly higher than other experimental groups.
Shown in attached drawing 17, with the increase of mountain flour additive amount, the variation of dry matter digestibility is not notable；Calcium digestibility
Variation, which is presented, first increases the trend subtracted afterwards, and from 4%-6.6%, calcium digestibility changes greatly additive amount, and is digested when 6.6%-8%
Rate tends towards stability.The mountain flour of additive amount 8% is significantly higher than other experimental groups, reaches maximum value 37.5%.
It takes influence of the mountain flour Different adding amount to feed Vitro Digestibility into consideration, determines that the requirement range of mountain flour is
7.5%-8.5%, it is 8% to select the Optimum Requirement of mountain flour.
3. the determination of phosphorus most expense
On the feed base of the cotton dregs of high protein containing 100g/kg, addition 1.2%, 1.3%, 1.5%, 1.6%, 1.7%
Calcium monohydrogen phosphate, influence of the research different phosphate additive amount to mixed feed digestibility, to choose most suitable Phosphorus requirement, optimization production
Feed formula for laying hen.
Influence referring to the phosphorus that attached drawing 18 is Different adding amount to external feed digestibility.
Shown in attached drawing 18, with the increase of phosphorus additive amount, diet dry matter digestion takes the lead in subtracting after increasing, additive amount
The Vitro Digestibility of 1.6% sample is significantly higher, and the variation of calcium digestibility is similar to the variation of dry matter digestibility, presents
First increase the trend subtracted afterwards, the phosphorus for adding 1.6% reaches maximum value 39.8%, is significantly higher than other experimental groups (P<0.05).
Consider influence of the phosphorus Different adding amount to feed Vitro Digestibility, determines the requirement range of calcium monohydrogen phosphate
It is 1.5%-1.7%.
4. the determination of lecithin requirement
On the feed base of the cotton dregs of high protein containing 100g/kg, 0%, 1%, 2%, 3%, 4% lecithin, research are added
Influence of the different accession capacity of Lecithin to mixed feed, to choose most suitable lecithin requirement, optimization layer chicken feed is matched
Side.Influence referring to the lecithin that attached drawing 19 is Different adding amount to feed Vitro Digestibility.
Shown in attached drawing 19, with the increase of accession capacity of Lecithin, protein, calcium digestibility are changed significantly, and present first
The trend subtracted after increasing, when accession capacity of Lecithin is 2%, protein, calcium digestibility are significantly higher than other experimental groups, reach most
Big value 0.61,0.41.
As described above, you can preferably realize that the present invention, the above embodiments are only the preferred implementation side to the present invention
Formula is described, and is not defined to the scope of the present invention, under the premise of not departing from design spirit of the present invention, this field
The various modifications and improvement that those of ordinary skill makes technical scheme of the present invention, should all fall into present invention determine that protection
In range.
Claims (7)
1. a kind of layer chicken feed of high protein cotton dregs Substitution for Soybean Meal, which is characterized in that the layer chicken feed basal feed with
Weight percent is calculated as：Dregs of beans 12.6%, cotton dregs 10%, mountain flour 7.5%-8.5%, calcium monohydrogen phosphate 1.5%-2%, premix 1%, plant
Oil 1%, salt 0.3%, zeolite powder 2%-2.6%, lysine 0.1%, ferrous sulfate 0.002%, remaining is corn；
It is corresponded to per kg cotton dregs in above-mentioned basal feed and feed addictive keratinase 0.8g-1.2g is added, in above-mentioned basal feed
It is corresponded to per kg cotton dregs and feed addictive lecithin 15g-25g is added.
2. the layer chicken feed of high protein cotton dregs Substitution for Soybean Meal as described in claim 1, which is characterized in that the high protein cotton
The crude protein content of the dregs of rice is 55%, free gossypol 160mg/kg.
3. the layer chicken feed of high protein cotton dregs Substitution for Soybean Meal as described in claim 1, which is characterized in that the feed addition
Prolease activity >=150000U/g of agent keratinase.
4. the layer chicken feed of high protein cotton dregs Substitution for Soybean Meal as described in claim 1, which is characterized in that the laying hen is raised
Expect that basal feed is by weight percentage：Dregs of beans 12.6%, mountain flour 8%, calcium monohydrogen phosphate 1.7%, premix 1%, is planted at cotton dregs 10%
Object oil 1%, salt 0.3%, zeolite powder 2.5%, lysine 0.1%, ferrous sulfate 0.002%, remaining is corn.
5. such as the layer chicken feed of claim 4 any one of them high protein cotton dregs Substitution for Soybean Meal, which is characterized in that the base
It is corresponded to per kg cotton dregs in plinth feed and feed addictive keratinase 1g is added.
6. such as the layer chicken feed of claim 4 any one of them high protein cotton dregs Substitution for Soybean Meal, which is characterized in that the base
It is corresponded to per kg cotton dregs in plinth feed and feed addictive lecithin 20g is added.
7. the layer chicken feed of high protein cotton dregs Substitution for Soybean Meal as described in claim 1, which is characterized in that the high protein cotton
The dregs of rice are the cotton dregs by crushing and crossing 40-60 mesh sieve.
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| CN112219947A (en) * | 2020-09-29 | 2021-01-15 | 新疆希普生物科技股份有限公司 | Composition containing cottonseed protein powder and preparation method thereof |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A High Protein Cotton Meal as a Substitute for Soybean Meal in Egg laying Chicken Feed and Its Preparation Effective date of registration: 20231218 Granted publication date: 20211109 Pledgee: Agricultural Development Bank of China Changji Hui Autonomous Prefecture Branch Business Department Pledgor: XINJIANG TAIKUN Group LLC Registration number: Y2023980071914 |