CN104304819A - Heat-stress-resistant fermented feed for laying hen and preparation method thereof - Google Patents
Heat-stress-resistant fermented feed for laying hen and preparation method thereof Download PDFInfo
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Abstract
The invention discloses heat-stress-resistant fermented feed for a laying hen. The heat-stress-resistant fermented feed is prepared from the following raw materials: corn, soybean meal, cottonseed meal, apple pomace, chili meal, corn steep liquor sprayed corn husk, wheat bran, bacteria liquid, compound premixed feed and water, wherein the bacteria liquid is prepared from enterococcus aureus, bacillus subtilis, saccharomyces cerevisiae, brown sugar and water. The preparation method of the heat-stress-resistant fermented feed comprises the following steps: crushing the raw material, mixing the dried material, preparing the bacteria liquid, secondarily mixing the wet feed, and conducting anaerobic fermentation. The heat-stress-resistant fermented feed disclosed by the invention has the following benefits: the main essential nutrients in the fermented feed are approximate to that of complete feed for the laying hen and comprise active beneficial bacteria, gamma-aminobutyric acid, organic acid and other active factors; the feed can be added to the daily ration of the laying hen based on the rate of 5-15% to obviously improve the laying rate of the laying hen, the feed-gain ratio, the broken egg rate and the oxidation resistance, synergistically give play to multiple action of the beneficial bacteria on microecologic control to bacterial flora in the gastrointestinal tract, nerve regulation to the central nervous system by the neurotransmitter, and buffering of the organic acid to respiratory alkalosis due to high temperature, and obviously resist against the heat stress due to high temperature in summer for the laying hen.
Description
Technical field
The present invention relates to poultry feed and preparation method thereof, be specifically related to a kind ofly be exclusively used in fermented feed of laying hen anti-heat stress and preparation method thereof.
Background technology
Laying hen body fever, metabolism is vigorous, and feather is abundant, but laying hen does not have sweat gland, under hot conditions with the waste heat produced in respiratory tract evaporative heat loss releaser to balance body temperature, therefore high temperature on production performance of layer chicken impact very large.The suitable environment temperature of layer breeding is 15 ~ 25 DEG C, just can produce heat stress more than 25 DEG C; More than 28 DEG C, the harm of heat stress is by highly significant.Heat stress not only makes laying rate of laying hen decline, and egg size alleviates, and rate of broken eggs increases, and fertility rate of hatching egg and incubation rate reduce, and can cause the even large quantities of death of the premunition of chicken and immunity degradation, cause huge economic loss to breeding layer chicken.
At present, alleviate the nutrition technique measure that mainly adopts of heat stress of layer: 1. improve layer diets energy concentration: during laying hen generation heat stress, in layer diets, add 0.5 ~ 2% grease, overcome and to be searched for food deficiency and the production performance that causes reduces by feed; Grease can reduce the intestine evacuation velocity of feed in esophagus, extends food elk time of staying in alimentary canal, makes Digestibility and absorbs more complete; The heat increment that grease also produces because of metabolism is few, thus slows down solution metabolic stress.2. low-protein daily ration is prepared: laying-hen compound feed daily ration level is reduced to 15.0 ~ 15.5%, then adds limiting amino acid, harmful gas concentration in the death rate and Laying House can be reduced.3. replenishing vitamins: add 8000 IU/kg vitamin As in layer diets, alleviates the adverse effect of heat stress to Layer Production Performance, improves laying hen immunologic function, carries out immunity inoculation and obtain higher antibody horizontal under ensureing hot environment; Add 50 IU/kg vitamin Es, reduce the too much Ca of interference eubolism in cell
2+inflow, strengthen immunity of organisms and effectively improve heat resistance; Add 100 ~ 200 mg/kg vitamin Cs, maintain the higher feed intake of laying hen, improve chicken body heat resistance.4. regulate electrolyte balance: add 0.1 ~ 0.2%NaHCO3,0.1 ~ 0.2%KCl in laying-hen compound feed, the amount of drinking water of chicken and the formation of urine can be increased, be beneficial to heat radiation and recover ionic equilibrium.5. add heat-clearing Chinese herbal medicine: can be antibacterial, promote peripheral vascular expansion, acceleration bodies Thermal release, increase vasopermeability, reduce blood volume, reduce brain intracranial pressure, dilute blood, reach the effect alleviating heat stress.6. acidulant is added: adding the anti-heat stress of layer reaction of the organic acid such as 0.1 ~ 0.2% citric acid and fumaric acid in laying-hen compound feed also has good result.7. mend and add probiotics: add 200 ~ 500g/t compound beneficial bacterium in laying-hen compound feed, to heat stress, there is certain mitigation.8. integrated nutrition comprehensive regulation.
In layer diets, add grease, a kind of simple and regular technology that replenishing vitamins, adjustment electrolyte balance have become anti-heat stress of layer and limited efficacy, the low-protein daily ration preparation technique adopted can have a negative impact again to Layer Production Performance because reducing Different Dietary Protein Levels, the use cost of Chinese herbal medicine and acidulant is higher and effect is also undesirable, Tiny ecosystem dry powder formulations because of addition too low and hardly containing viable bacteria metabolite and affect its effect, integrated nutrition comprehensive regulation makes its cost performance not high and effect is very not remarkable yet because of high cost.As can be seen here, in practice is produced, it is limited for adopting abovementioned technology to alleviate heat stress of layer.
The main mechanism of laying hen generation heat stress is because laying hen lacks sweat gland, high temperature makes laying hen significantly be strengthened by respiratory tract evaporative heat loss, overventilation causes the decline of CO2 content, pH value in blood to raise, cause caused by metabolic alkalosis, thus cause that acid-base balance is destroyed, electrolyte balance is unbalance further, endocrine dysfunction imbalance, metabolic disorder, degradation series of symptoms under immunity function.Therefore, how to solve metabolic alkalosis, regulate the problems such as body allomeric function state to become to alleviate laying hen high-temperature hot stress key.
Summary of the invention
The object of this invention is to provide a kind of laying hen anti-heat stress fermented feed and preparation method thereof, its form is wet basis state, containing the functional activity factor such as viable bacteria, GABA, lactic acid intact in a large number, to superpose the double effects playing fermented feed probiotics regulating and controlling effect and neurotransmitter GABA resisting stress, the harm that under effective alleviation laying hen hot conditions, heat stress produces, prevents the decline of Layer Production Performance.
Realizing the technical scheme that the object of the invention adopts is:
Laying hen provided by the invention anti-heat stress fermented feed, by gross weight 1000 parts, comprise following raw material: corn 50 ~ 200 parts, dregs of beans 50 ~ 150 parts, Cottonseed Meal 50 ~ 200 parts, pomace 30 ~ 100 parts, chili meal 0 ~ 100 part, whitewashing maize peel 30 ~ 120 parts, wheat bran 100 ~ 250 parts, bacterium liquid 30 ~ 50 parts, compound premixed feed 15 ~ 25 parts, 200 ~ 300 parts, water.
By gross weight 1000 parts, consisting of of described bacterium liquid: enterococcus faecalis F6 5 ~ 15 parts, bacillus subtilis 5 ~ 10 parts, S. cervisiae 5 ~ 10 parts, 50 ~ 100 parts, brown sugar, 865 ~ 935 parts, water.
Wherein, enterococcus faecalis F6(
enterococcus faecium F6) in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation registration, numbering of registering on the books: CGMCC No. 8538, applicant has perfect knowledge property right.In the fermentation medium of the sodium glutamate (monosodium) containing 35 g/L, 34 DEG C of quiescent culture 65 h, the output of this bacterial strain GABA reaches 7.1 g/L.Utilize seed selection to obtain the functional characteristic of the highly producing gamma-aminobutyric acid of this bacterium, by solid anaerobic digestion, tunning has beneficial bacterium lactic acid bacteria and the neurotransmitter GABA of high-load concurrently, and superposition plays its unique and significant anti-heat stress effect.Enterococcus faecalis F6 is containing viable bacteria 500,000,000 cfu/g.
Wherein, bacillus subtilis (
bacillus subtilis subsp. Subtilis) and S. cervisiae (
saccharomyces cerevisiae) all deriving from China Committee for Culture Collection of Microorganisms's common micro-organisms center, numbering of registering on the books is respectively: CGMCC2. 1882 and CGMCC No. 1946.These Bacillus subtilis subspecies have the ability of secreting bacteria element, but can harmful pathogenic microorganism such as Escherichia coli and salmonella be killed, and the growth metabolism of saccharomycete and lactic acid bacteria is not affected, stronger to the lethality of Escherichia coli and salmonella in slant acidity environment, thus reduce the damaging effect of heat stress of layer secondary hypoimmunity.This saccharomycete glycolysis is very capable, can tolerate lower pH environment, can also carry out glycometabolism when pH value is 4.5, and secretion produces the various active compositions such as vitamin B complex, nucleotides, unknown somatomedin, has better effects to alleviation heat stress of layer.Bacillus subtilis is containing viable bacteria 20,000,000,000 cfu/g, and S. cervisiae is containing viable bacteria 200,000,000 cfu/g.
The composition of described compound premixed feed: by gross weight 1000 parts, trace element premixing feed 60 ~ 100 parts, vitamin premixed feed 10 ~ 18 parts, stone flour 200 ~ 300 parts, calcium monohydrogen phosphate 400 ~ 500 parts, complex enzyme 10 ~ 15 parts, phytase 5 ~ 10 parts, the L lysine HCL 20 ~ 40 parts containing lysine 78%, DL-METHIONINE 20 ~ 80 parts, threonine 20 ~ 40 parts, salt 30 ~ 50 parts, the Choline Chloride 10 ~ 20 parts containing choline 50%, ethoxyquinoline 0.5 ~ 1.0 part, powdered rice hulls 0 ~ 10 part, zeolite powder 0 ~ 50 part.
Wherein, consisting of of described trace element premixing feed: by gross weight 1000 parts, ferrous sulfate monohydrate 140 ~ 200 parts, cupric sulfate pentahydrate 15 ~ 50 parts, monohydrate zinc sulphate 120 ~ 170 parts, manganese sulfate monohydrate 140 ~ 180 parts, a water sodium selenite 15 ~ 25 parts, calcium iodate 15 ~ 30 parts, cobalt chloride 5.0 ~ 30 parts, ethoxyquinoline 0.5 ~ 2.0 part, zeolite powder 313.0 ~ 544.5 parts.The content of trace element premixing feed each component is as follows: iron content 30% in ferrous sulfate monohydrate, cupric 25% in cupric sulfate pentahydrate, containing zinc 35% in monohydrate zinc sulphate, containing manganese 32% in manganese sulfate monohydrate, containing selenium 1% in one water sodium selenite, containing iodine 1% in calcium iodate, containing cobalt 1% in cobalt chloride, the mass fraction of ethoxyquinoline is 30%.
Consisting of of described vitamin premixed feed: by gross weight 1000 parts, retinyl acetate 60 ~ 80 parts, vitamin D
312 ~ 30 parts, vitamin E 100 ~ 200 parts, menadione sodium bisulfite 5.0 ~ 15 parts, thiamine hydrochloride 7.0 ~ 10 parts, Riboflavin Tetrabutyrate 5 ~ 45 parts, puridoxine hydrochloride 15 ~ 20 parts, Cobastab
125.0 ~ 8.0 parts, D-VB5 calcium 30 ~ 55 parts, niacinamide 90 ~ 130 parts, 3.5 ~ 6.0 parts, folic acid, Bio 25 ~ 35 parts, ethoxyquinoline 0.5 ~ 2.0 part, powdered rice hulls 364.0 ~ 622.0 parts.The effective content of vitamin premixed feed each component is as follows: retinyl acetate biological value is 500,000 IU/g
,vitamin D
3biological value is 500,000 IU/g, and the mass fraction of vitamin E is 50%, and the mass fraction of menadione sodium bisulfite is 50%, and the mass fraction of thiamine hydrochloride is 98%, and the mass fraction of riboflavin is 80%, and the mass fraction of puridoxine hydrochloride is 98%, Cobastab
12mass fraction be 1%, the mass fraction of D-VB5 calcium is 98%, and the mass fraction of niacinamide is 99%, and the mass fraction of folic acid is the mass fraction of 98%, D-bionic acid is 2%, and the mass fraction of ethoxyquinoline is 30%.
Consisting of of described complex enzyme: by gross weight 1000 parts, cellulase 100 parts, zytase 500 parts, acid protease 200 parts, AMS 100 parts, powdered rice hulls 100 parts.The activity of complex enzyme each component is as follows: the activity of various enzyme is: cellulase 10000U/g, zytase 10000U/g, acid protease 50000U/g, AMS 4000U/g.
The laying hen provided by the invention preparation method of anti-heat stress fermented feed: (1) raw material is pulverized: by other raw materials beyond bacteria-removing liquid, compound premixed feed and water in raw material through weighing, measuring, feed intake, promote, pulverize after removal of impurities, the raw material after pulverizing is by diameter 1.2 ~ 2.5mm sieve aperture; (2) siccative mixing: by the raw material after pulverizing and compound premixed feed Homogeneous phase mixing, obtain mixed material; (3) bacterium solution preparation: enterococcus faecalis F6, bacillus subtilis, S. cervisiae and brown sugar, water Homogeneous phase mixing are made bacterium liquid, keeps 35 ~ 45 DEG C of water temperature 0.5 ~ 1.0 h, obtains the bacterium liquid activated; (4) secondary wet feed mixing: by bacterium liquid, water, the mixed material Homogeneous phase mixing of activation, obtain mixed material; (5) anaerobic fermentation: by mixed material anaerobic fermentation 5 ~ 8 days at 18 ~ 45 DEG C, obtain anti-heat stress fermented feed.
The Major Nutrient level of the above-mentioned anti-heat stress fermented feed prepared is (wet basis): moisture 30 ~ 45%, metabolizable energy 1400 ~ 1700 kcal/kg, protein 13.0 ~ 16.5%, calcium 0.50 ~ 0.70%, total phosphorus 0.55 ~ 0.75%, lysine 0.60 ~ 0.8%, methionine 0.30 ~ 0.40%, threonine 0.40 ~ 0.55%.
Other active component content in the above-mentioned anti-heat stress fermented feed prepared is (wet basis): GABA 0.25 ~ 0.40g/kg, and enterococcus faecalis is containing viable bacteria 1.0 × 10
8~ 2.0 × 10
8cfu/g, total viable count 5.0 × 10
8cfu/g.
Fermentative feedstuff of microbe is a kind of biological feedstuff of high-quality, the beneficial bacterium that wet basis state fermented feed can provide high-load to live and microbial activity metabolite, as: lactic acid, enzyme, the Immune-enhancing effect factor, unknown somatomedin etc., wherein organic acid is as buffer, contribute to the pH value maintained in blood to stablize, effectively can prevent respiratory alkalosis, and can feed intake be improved; The adjustable gut flora balance of other active factors, the clean intestines of stomach invigorating, strengthen immunity.And its interpolation application cost is lower.
In addition, GABA is a kind of neurotransmitter, and energy modulation of appetite, adjustment blood pressure, promotion sleep, especially have calm, anti-stress effect.If containing maybe producing Antistress agent GABA in fermented feed, it will be very remarkable to anti-heat stress effect of laying hen.
The beneficial effect that the present invention has:
Existing market is still rarely found, and to have to produce GABA lactic acid bacteria be that main beneficial bacterium ferments the wet basis state fermented feed obtained, and the present invention has following beneficial functional:
(1) by the common fermentation of three kinds of profitable strains, in the fermented feed product obtained, 5.0 × 10 are reached containing high viable count
8cfu/g, wherein enterococcus faecalis viable bacteria content reaches 1.0 × 10
8~ 2.0 × 10
8cfu/g, and account for 5 ~ 15% of laying-hen compound feed due to the addition of fermented feed, the beneficial bacterium therefore being entered the work of small intestine by stomach still has comparatively high amts, is not less than 70% before stomach.The beneficial bacterium of high-load improves the micro-ecological environment of enteron aisle, strengthens the immunologic function of body, regulates the allomeric function state of body, significantly improves the absorption rate to nutriment, thus slow down the heat stress of laying hen.
(2) lactic acid bacteria of the present invention's screening is the unique functionality bacterial strain of 1 strain highly producing gamma-aminobutyric acid enterococcus faecalis, not only productive rate is high when solid anaerobic digestion for this bacterial strain, and can GABA be produced, make the alpha-aminobutyric acid content in fermented feed reach 0.25g ~ 0.40 g/kg.GABA is a kind of neurotransmitter, and have the maincenter function immunoregulation functions such as stronger calmness, resisting stress and improving water flood, superpose with the micro ecology of gastrointestinal tract Balanceregulation of beneficial bacterium, its anti-heat stress of layer effect is very remarkable.
(3) fermented feed obtained under the strict environment controlled, metabolite also containing a large amount of beneficial bacteriums, as: lactic acid, B family vitamin, complex enzyme, nucleotides, unknown somatomedin etc., especially the organic acid content such as lactic acid can reach 0.4 ~ 2.0%, pH value 3.8 ~ 4.5.Wherein, organic acid is oxidized into CO in laying hen body
2and bicarbonate, as the buffer in blood, contribute to the pH value maintained in blood and stablize, prevent the harm of respiratory alkalosis, alleviate the impact of heat stress on laying hen from source.
(4) also additionally with the addition of the endogenous enzymes such as exogenous enzymes and acid protease such as cellulase in fermented feed, these complex enzymes can give full play to its degradation to nutriment in the sweat of 5 ~ 8 days, contribute to improving laying hen absorbing nutriment.
(5) preparation of fermented feed adopts strict, standardized process conditions, fermented product steady quality, and the holding time is 1 year, and viable bacteria loss is not higher than 10%.
(6) nutritive index in the Major Nutrient index of fermented feed and laying-hen compound feed is comparatively close, therefore, ratio with 5 ~ 15% is added in layer diets in a large number, while performance Effective Anti heat stress of layer, also ensure that it meets the demand of laying hen nutrition.
(7) fermented feed of the present invention is used, the egg laying performance of laying hen under 32 DEG C of hot environments is not affected, the harmful gas concentrations such as hen house ammonia reduce by 40 ~ 60%, chicken group is healthy, welfare, show that laying hen anti-heat stress ability significantly strengthens, effectively prevent the adverse effect that heat stress brings to breeding layer chicken.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
The laying hen that the present embodiment provides anti-heat stress fermented feed, by gross weight 1000 parts, is made up of following raw material:
Corn 100 parts, dregs of beans 100 parts, Cottonseed Meal 100 parts, pomace 80 parts, chili meal 70 parts, whitewashing maize peel 80 parts, wheat bran 150 parts, bacterium liquid 40 parts, compound premixed feed 20 parts, 260 parts, water.
In the present embodiment, described bacterium liquid composition: by gross weight 1000 parts, 15 parts, enterococcus faecalis F6 bacterium powder, 5 parts, bacillus subtilis bacterium powder, 5 parts, S. cervisiae bacterium powder, 75 parts, brown sugar, 900 parts, water.Enterococcus faecalis, bacillus subtilis, S. cervisiae viable count are respectively 500,000,000 cfu/g, 20,000,000,000 cfu/g, 200,000,000 cfu/g.
In the present embodiment, described compound premixed feed composition: by gross weight 1000 parts, trace element premixing feed 80 parts, vitamin premixed feed 12 parts, stone flour 228 parts, calcium monohydrogen phosphate 460 parts, complex enzyme 12 parts, phytase 7 parts, L lysine HCL (containing lysine 78%) 30 parts, DL-METHIONINE 70 parts, threonine 30 parts, salt 40 parts, Choline Chloride (containing choline 50%) 15 parts, ethoxyquinoline 1.0 parts, powdered rice hulls 5 parts, zeolite powder 10 parts.
Wherein, described trace element premixing feed composition: by gross weight 1000 parts, ferrous sulfate monohydrate 160 parts, cupric sulfate pentahydrate 25 parts, monohydrate zinc sulphate 140 parts, manganese sulfate monohydrate 150 parts, a water sodium selenite 22 parts, calcium iodate 25 parts, cobalt chloride 10 parts, ethoxyquinoline 1.0 parts, zeolite powder 467 parts.
Wherein, described vitamin premixed feed composition: by gross weight 1000 parts, retinyl acetate 76 parts, vitamin D
323 parts, vitamin E 160 parts, menadione sodium bisulfite 10 parts, thiamine hydrochloride 8.0 parts, 38 parts, riboflavin, puridoxine hydrochloride 17 parts, Cobastab
126.0 parts, D-VB5 calcium 42 parts, niacinamide 105 parts, 4.5 parts, folic acid, Bio 30 parts, ethoxyquinoline 1.0 parts, powdered rice hulls 479.5 parts.
Wherein, by gross weight 1000 parts, consisting of of described complex enzyme: cellulase 100 parts, zytase 500 parts, acid protease 200 parts, AMS 100 parts, powdered rice hulls 100 parts.
In the present embodiment, the method preparing laying hen anti-heat stress fermented feed has following steps:
(1) by corn, dregs of beans, Cottonseed Meal, pomace, chili meal, whitewashing maize peel, wheat bran Weighing respectively, feed intake respectively in dog-house, after bucket elevator vertical-lift, enter cylindrical pre-cleaning screen dedusting impurity elimination, enter pipe magnetic separator again and wait magnetic components except de-iron is assorted, then enter pulverizer respectively and pulverize.Pulverizer is beater disintegrating machine, and sieve diameter is 1.2 ~ 2.5mm.Corn, dregs of beans, Cottonseed Meal, the chili meal pulverizer of sieve diameter 1.5mm is pulverized, and pomace, whitewashing maize peel, the wheat bran pulverizer of sieve diameter 2.0mm are pulverized.
(2) by the raw material after above-mentioned pulverizing and compound premixed feed bucket elevator vertical-lift extremely storehouse to be mixed, enter horizontal-type biaxial paddle type mixer, mix 3 minutes.
(3) in fluid reservoir, the dry bacterium powder of enterococcus faecalis F6, bacillus subtilis, S. cervisiae three bacterial classifications and brown sugar, water are stirred and evenly mixed according to the ratio specified, makes bacterium liquid, and keep 40 DEG C of water temperatures 45 minutes, make bacterial classification activity recovery, obtain the bacterium liquid of activation.
(4) the bacterium liquid after activation is delivered to mixer through fluid pipeline, becomes fine liquid particles in the material of mixer by sprinkler, sprinkling limit, limit is stirred, incorporation time 3 minutes, after requiring that all bacterium liquid is sprayed, then adds water, mix 2 minutes again, obtain mixed material.
(5) be packaged in packaging bag by the material after above-mentioned inoculating strain mixing through discharging opening, this packaging bag liner some strength and flexible bag film, outer bag is coated knitted bag; Discharge air in bag, tighten sack; Then in the environment of 25 DEG C, carry out anaerobic fermentation 7 days, prepare anti-heat stress fermented feed product.
The fermented feed quality judging standard prepared by said process:
1. smell Judging index: pure sour fragrance mixes wine flavour, the acomia bad smell that goes mouldy.2. appearance determination index: color and luster consistent and uniform, not lump, without white point mould contamination.3. moisture Judging index: material loosening, humidity requires that hand is grabbed agglomerating, but scatters at once after loosing one's grip.4. pH value Judging index: pH value, not higher than 4.5, has obvious tart flavour for standard with mouthfeel.5. pack Judging index: package encapsulation is tight, visual inspection is without damaged, air tight.Allly meet above-mentioned condition, the quality of the fermented feed made is qualified or reach gold standard.
The Major Nutrient level of the above-mentioned anti-heat stress fermented feed that the present embodiment prepares is (wet basis): moisture 38.6%, metabolizable energy 1501 kcal/kg, protein 14.6%, calcium 0.54%, total phosphorus 0.63%, lysine 0.70%, methionine 0.32%, threonine 0.46%.
The anti-heat stress ferment feed (hereinafter referred to as fermented feed) that the present embodiment obtains can be mixed with layer diets with the raw material such as 5% laying hen compound premixed feed and corn, and by gross weight number 1000, the proportioning of this layer diets is:
Corn (crude protein 8.0%) 560 parts, dregs of beans (crude protein 43.5%) 210 parts, 100 parts, fermented feed, stone flour 80 parts, 5% laying hen compound premixed feed 50 parts.
The Major Nutrient level of the above-mentioned layer diets prepared is (wet basis): moisture 13.9%, metabolizable energy 2598 kcal/kg, protein 15.6%, calcium 3.85%, total phosphorus 0.52%, lysine 0.79%, methionine 0.38%, threonine 0.62%.
Embodiment 2
The laying hen that the present embodiment provides anti-heat stress fermented feed, by gross weight 1000 parts, is made up of following raw material:
Corn 150 parts, dregs of beans 80 parts, Cottonseed Meal 80 parts, pomace 100 parts, chili meal 50 parts, whitewashing maize peel 100 parts, wheat bran 120 parts, bacterium liquid 35 parts, compound premixed feed 15 parts, 270 parts, water.
In the present embodiment, described bacterium liquid composition: by gross weight 1000 parts, 10 parts, enterococcus faecalis F6 bacterium powder, 7.5 parts, bacillus subtilis bacterium powder, 7.5 parts, S. cervisiae bacterium powder, 50 parts, brown sugar, 925 parts, water.Enterococcus faecalis, bacillus subtilis, S. cervisiae viable count are respectively 500,000,000 cfu/g, 20,000,000,000 cfu/g, 200,000,000 cfu/g.
In the present embodiment, described compound premixed feed composition: by gross weight 1000 parts, trace element premixing feed 70 parts, vitamin premixed feed 10 parts, stone flour 210 parts, calcium monohydrogen phosphate 490 parts, complex enzyme 10 parts, phytase 5 parts, L lysine HCL (containing lysine 78%) 25 parts, DL-METHIONINE 60 parts, threonine 20 parts, salt 30 parts, Choline Chloride (containing choline 50%) 10 parts, ethoxyquinoline 0.5 part, powdered rice hulls 10 parts, zeolite powder 49.5 parts.
Wherein, described trace element premixing feed composition: by gross weight 1000 parts, ferrous sulfate monohydrate 150 parts, cupric sulfate pentahydrate 10 parts, monohydrate zinc sulphate 130 parts, manganese sulfate monohydrate 140 parts, a water sodium selenite 15 parts, calcium iodate 20 parts, cobalt chloride 5.0 parts, ethoxyquinoline 0.5 part, zeolite powder 529.5 parts.
Wherein, described vitamin premixed feed composition: by gross weight 1000 parts, retinyl acetate 70 parts, vitamin D
315 parts, vitamin E 120 parts, menadione sodium bisulfite 5.0 parts, thiamine hydrochloride 7.0 parts, 30 parts, riboflavin, puridoxine hydrochloride 15 parts, Cobastab
125.0 parts, D-VB5 calcium 35 parts, niacinamide 95 parts, 4.0 parts, folic acid, Bio 25 parts, ethoxyquinoline 0.5 part, powdered rice hulls 573.5 parts.
Wherein, the composition of described complex enzyme is identical with embodiment 1.
In the present embodiment, the preparation method of fermented feed and step ditto described in, the design parameter condition of each step is:
(1) pulverize: corn, dregs of beans, Cottonseed Meal, the chili meal pulverizer of sieve diameter 1.2mm is pulverized, pomace, whitewashing maize peel, the wheat bran pulverizer of sieve diameter 2.0mm are pulverized.(2) raw material after pulverizing mixes 2.5 minutes in horizontal-type biaxial paddle type mixer.(3) bacterium liquid keeps 38 DEG C of water temperatures 35 minutes.(4) sprinkling limit, bacterium liquid limit is stirred, and incorporation time 2.5 minutes, after all bacterium liquid is sprayed, then adds water, mixes 2 minutes, totally 4.5 minutes.(5) in 20 DEG C of environment, anaerobic fermentation 8 days.
The fermented feed quality judging standard prepared by said process is with embodiment 1.
The Major Nutrient level of the above-mentioned anti-heat stress fermented feed that the present embodiment prepares is (wet basis): moisture 39.1%, metabolizable energy 1552 kcal/kg, protein 13.0%, calcium 0.42%, total phosphorus 0.60%, lysine 0.60%, methionine 0.27%, threonine 0.41%.
The anti-heat stress ferment feed (hereinafter referred to as fermented feed) that the present embodiment obtains can be mixed with layer diets with the raw material such as 5% laying hen compound premixed feed and corn, and by gross weight number 1000, the proportioning of this layer diets is:
Corn (crude protein 8.0%) 560 parts, dregs of beans (crude protein 43.5%) 210 parts, 100 parts, fermented feed, stone flour 80 parts, 5% laying hen compound premixed feed 50 parts.
The Major Nutrient level of the above-mentioned layer diets prepared is (wet basis): moisture 14.5%, metabolizable energy 2562 kcal/kg, protein 15.2%, calcium 3.85%, total phosphorus 0.51%, lysine 0.76%, methionine 0.37%, threonine 0.60%.
Embodiment 3
The laying hen that the present embodiment provides anti-heat stress fermented feed, by gross weight 1000 parts, is made up of following raw material:
Corn 80 parts, dregs of beans 120 parts, Cottonseed Meal 140 parts, pomace 70 parts, chili meal 60 parts, whitewashing maize peel 60 parts, wheat bran 150 parts, bacterium liquid 45 parts, compound premixed feed 25 parts, 250 parts, water.
In the present embodiment, described bacterium liquid composition: by gross weight 1000 parts, 13 parts, enterococcus faecalis bacterium powder, 6 parts, bacillus subtilis bacterium powder, 6 parts, S. cervisiae bacterium powder, 100 parts, brown sugar, 875 parts, water.Enterococcus faecalis, bacillus subtilis, S. cervisiae viable count are respectively 500,000,000 cfu/g, 20,000,000,000 cfu/g, 200,000,000 cfu/g.
In the present embodiment, described compound premixed feed composition: by gross weight 1000 parts, trace element premixing feed 85 parts, vitamin premixed feed 15 parts, stone flour 200 parts, calcium monohydrogen phosphate 470 parts, complex enzyme 15 parts, phytase 8.0 parts, L lysine HCL (containing lysine 78%) 35 parts, DL-METHIONINE 75 parts, threonine 35 parts, salt 40 parts, Choline Chloride (containing choline 50%) 15 parts, ethoxyquinoline 1.0 parts, zeolite powder 6.0 parts.
Wherein, described trace element premixing feed composition: by gross weight 1000 parts, ferrous sulfate monohydrate 180 parts, cupric sulfate pentahydrate 35 parts, monohydrate zinc sulphate 160 parts, manganese sulfate monohydrate 170 parts, a water sodium selenite 25 parts, calcium iodate 30 parts, cobalt chloride 20 parts, ethoxyquinoline 1.5 parts, zeolite powder 378.5 parts.
Wherein, described vitamin premixed feed composition: by gross weight 1000 parts, retinyl acetate 80 parts, vitamin D
325 parts, vitamin E 180 parts, menadione sodium bisulfite 10 parts, thiamine hydrochloride 10 parts, 45 parts, riboflavin, puridoxine hydrochloride 20 parts, Cobastab
127.0 parts, D-VB5 calcium 50 parts, niacinamide 120 parts, 5.0 parts, folic acid, Bio 35 parts, ethoxyquinoline 1.5 parts, powdered rice hulls 411.5 parts.
Wherein, the composition of described complex enzyme is identical with embodiment 1.
In the present embodiment, the preparation method of fermented feed and step ditto described in, the design parameter condition of each step is:
(1) pulverize: corn, dregs of beans, Cottonseed Meal, the chili meal pulverizer of sieve diameter 1.2mm is pulverized, pomace, whitewashing maize peel, the wheat bran pulverizer of sieve diameter 2.0mm are pulverized.(2) raw material after pulverizing mixes 3.5 minutes in horizontal-type biaxial paddle type mixer.(3) bacterium liquid keeps 43 DEG C of water temperatures 50 minutes.(4) sprinkling limit, bacterium liquid limit is stirred, and incorporation time 3.5 minutes, after all bacterium liquid is sprayed, then adds water, mixes 2.5 minutes, totally 6 minutes.(5) in 30 DEG C of environment, anaerobic fermentation 6 days.
The fermented feed quality judging standard prepared by said process is with embodiment 1.
The Major Nutrient level of the above-mentioned anti-heat stress fermented feed that the present embodiment prepares is (wet basis): moisture 37.9%, metabolizable energy 1493 kcal/kg, protein 16.5%, calcium 0.65%, total phosphorus 0.73%, lysine 0.82%, methionine 0.38%, threonine 0.52%.
The anti-heat stress ferment feed (hereinafter referred to as fermented feed) that the present embodiment obtains can be mixed with layer diets with the raw material such as 5% laying hen compound premixed feed and corn, and by gross weight number 1000, the proportioning of this layer diets is:
Corn (crude protein 8.0%) 560 parts, dregs of beans (crude protein 43.5%) 210 parts, 100 parts, fermented feed, stone flour 80 parts, 5% laying hen compound premixed feed 50 parts.
The Major Nutrient level of the above-mentioned layer diets prepared is (wet basis): moisture 14.5%, metabolizable energy 2562 kcal/kg, protein 15.2%, calcium 3.85%, total phosphorus 0.51%, lysine 0.76%, methionine 0.37%, threonine 0.60%.
Feeding experiment
Carry out according to People's Republic of China's agricultural industry criteria-" fowl raising standard " (NY/T33 ~ 2004), process of the test and result as follows:
1, laying hen grouping
Test selection 290 age in days sea blue brown laying hen totally 1200, be divided into control group A and experiment group B, C, D tetra-processed group at random, often process 6 repetitions, often repeat 50, often organize totally 300, before on-test, carried out Continuous disinfection in hen house, test and carry out August in summer, altogether raise 4 weeks, experimental period hen house mean temperature be 32 DEG C.
2, daily ration is tested
Experimental basis daily ration is the perfect compound feed be mixed with by corn, dregs of beans, stone flour and 5% laying hen compound premixed feed, and control group A is fed basal diet; Experiment group B adopts the daily ration recommended in the embodiment of the present invention 1, namely corn, dregs of beans that 10% laying hen of the present invention anti-heat stress fermented feed substitutes different proportion is added, by the GABA of this fermented feed containing 0.35 g/kg, then containing GABA 35 g/t in the mixed feed be mixed with; Test group C adds the probiotics of 500g/t in basal diet, this probiotics comprise 200g/t containing viable bacteria 500,000,000 cfu/g Bacillus acidi lactici (can not GABA be secreted), 200g/t containing viable bacteria 20,000,000,000 cfu/g bacillus subtilis (
bacillus subtilis subsp. Subtilis), 100g/t containing viable bacteria 200,000,000 cfu/g S. cervisiae (
saccharomyces cerevisiae); Test group D adds in basal diet and in experiment group B, the chemical industry of contained equivalent synthesizes GABA 35 g/t.Test Diet Formula and trophic component are respectively in table 1,2.
Table 1 laying ration forms
| Raw material | A | B | C | D |
| Corn, 8% CP | 620 | 560 | 620 | 620 |
| Dregs of beans, 43.5% CP | 250 | 210 | 250 | 250 |
| Stone flour, 37% Ca | 80 | 80 | 80 | 80 |
| 5% laying hen compound premixed feed | 50 | 50 | 50 | 50 |
| Anti-heat stress fermented feed | - | 100 | - | - |
| Probiotics | - | - | 0.5 | - |
| GABA | - | - | - | 0.035 |
| Add up to | 1000 | 1000 | 1000.5 | 1000.035 |
Table 2 laying ration main nutrient composition and level
| Nutritive index | A | B | C | D |
| Metabolizable energy, kcal/kg | 2706 | 2598 | 2706 | 2706 |
| Thick protein, % | 16.3 | 15.6 | 16.3 | 16.3 |
| Calcium, % | 3.82 | 3.85 | 3.82 | 3.82 |
| Total phosphorus, % | 0.51 | 0.52 | 0.51 | 0.51 |
| Lysine, % | 0.83 | 0.79 | 0.83 | 0.83 |
| Methionine, % | 0.38 | 0.38 | 0.38 | 0.38 |
| Threonine, % | 0.66 | 0.62 | 0.66 | 0.66 |
3, feeding and management
Test laying hen adopts three stageds to raise in cages, 3, every cage, day feeding 2 times.Each processed group is in units of repeating, and arrangement of being separated by, to reduce the error that environmental condition causes.Laying hen free choice feeding, freely drinks water, illumination 16 h/d.Carry out ventilation in daily house, regularly clear excrement, routine immunization.Record the amount of drinking water of hen house, illumination, temperature, humidity every day.
4, Indexs measure
(1) egg laying performance index: every morning experimental period 8:30 and afternoon 2:30 observe the searching for food of laying hen, ight soil outward appearance, chicken group health status, medicine for treatment and deadly wash in a pan situation etc.Respectively observe to off-test every day at the upper and lower noon from on-test and pick up egg once, feed intake, egg number is recorded in units of repeating groups, weigh egg size, calculate the average egg production of each group of laying hen, average egg weight, average daily ingestion amount, feedstuff-egg ratio, rate of broken eggs.
Average egg production (%)=total egg number/(chicken number × number of days) × 100
Average egg weight (g/)=total egg size/(chicken number × number of days)
Average daily ingestion amount (g/ only)=total feed intake/(chicken number × number of days)
Feedstuff-egg ratio=average daily ingestion amount/average day egg production
Rate of broken eggs (%)=broken egg number/total egg number × 100
(2) Serum antioxidant indices measures: for examination laying hen off-test same day, gather a blood sample, every repeating groups gets 3 chickens, blood sampling was carried out under 8:00 fasted conditions in the morning, wing venous blood collection 2ml/, hold over night at 4 DEG C, centrifugal 10 minutes of 3000r/min, serum is prepared in absorption, and-20 DEG C of Refrigerator stores are for subsequent use, for measuring the content of MDA (MDA) in serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX).
MDA (MDA) measures: adopt thiobarbituricacidα-(Thibabituric Acid TBA) method.Measuring principle be MDA (MDA) in lipid peroxide catabolite can with thiobarbituricacidα-(TBA) condensation, form red product, have maximum absorption band at 523nm place, the content of MDA can be calculated according to OD value and standard liquid OD value.
Superoxide dismutase (SOD) measures: by adding the SOD enzyme liquid of different amount in reactant liquor, each liquid OD value under measuring 560nm wavelength after illumination certain hour, suppress NBT photo-reduction relative percentage and enzymatic activity proportional within the specific limits, with enzyme liquid addition for abscissa, to suppress NBT photo-reduction relative percentage for ordinate, graph paper is drawn out the two correlation curve, suppresses NBT photo-reduction relative percentage to calculate enzymatic activity according to SOD.
Glutathione peroxidase (GSH-PX) measures: because glutathione peroxidase (GSH-PX) can promote reduced glutathione (GSH) and H
2o
2water generation reaction and oxidized form of glutathione (GSSG).Therefore, the vigor of GSH-PX can represent by the speed of its enzymatic reaction, measures the consumption of reduced glutathione in this enzymatic reaction, then can obtain the vigor of enzyme.
5, data processing
Test data adopts SPSS 10.0 statistical software to add up, and carries out variance analysis and Duncan ' s Multiple range test.The significance level of difference sets:
p< 0.05 is significant difference,
p< 0.01 is that difference is extremely remarkable.
6, result
(1) egg laying performance: A, B, C, D tetra-processed group laying hens test the egg laying performance of 4 time-of-weeks in table 3.
Table 3 anti-heat stress fermented feed is on the impact of performance in layers
| Time | A | B | C | D |
| ? | ? | Average egg production, % | ? | ? |
| 1-2 week | 88.3 a | 90.7 c | 88.9 ab | 89.2 abc |
| 3-4 week | 88.1 a | 90.4 b | 89.0 ab | 89.6 ab |
| 1-4 week | 88.2 a | 90.6 c | 89.0 ab | 89.4 abc |
| ? | ? | Average egg weight, g/ | ? | ? |
| 1-2 week | 62.2 | 62.1 | 62.4 | 63.0 |
| 3-4 week | 62.5 | 62.6 | 62.5 | 62.6 |
| 1-4 week | 62.4 | 62.4 | 62.5 | 62.8 |
| ? | ? | Average daily ingestion amount, g/ only | ? | ? |
| 1-2 week | 110.8 | 109.7 | 112.3 | 111.9 |
| 3-4 week | 112.2 | 108.8 | 111.4 | 110.1 |
| 1-4 week | 111.5 | 109.3 | 111.9 | 111.0 |
| ? | ? | Feedstuff-egg ratio | ? | ? |
| 1-2 week | 2.02 a | 1.95 b | 2.02 a | 1.99 a |
| 3-4 week | 2.04 a | 1.92 c | 2.00 ab | 1.96 abc |
| 1-4 week | 2.03 a | 1.94 c | 2.01 ab | 1.98 abc |
Note: 1, be the average of each processed group above.
2, data of going together shoulder note same letter represent difference not significantly (
p> 0.05), shoulder note different lowercase alphabet show significant difference (
p< 0.05); Different uppercase expression difference extremely significantly (
p< 0.01), lower same.
The average egg production result display of different disposal group: test 1-2 is all, 3-4 is all, all the highest with B group laying rate, and laying rate is D, C, A group from high to low successively; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D laying rate improve 2.66%(respectively
p< 0.05), 0.85%(
p> 0.05), 1.36%(
p> 0.05), experiment group B laying rate comparatively test group C significantly improves 1.80%(
p< 0.05).Between test group C and D feedstuff-egg ratio without significant difference (
p> 0.05).
The egg size of different disposal group and the display of average daily feed intake result: test 1-2 week, 3-4 all and 1-4 thorough phase, the average egg weight between control group A group and test B, C, D respectively organize is all close with average daily ingestion amount, have no significant difference (
p> 0.05).
The feedstuff-egg ratio result display of different disposal group: test 1-2 is all, 3-4 is all, all minimum with B group feedstuff-egg ratio, and feedstuff-egg ratio is D, C, A group from low to high successively; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D feedstuff-egg ratio reduce 4.56%(respectively
p< 0.05), 0.69%(
p> 0.05), 2.49%(
p> 0.05); Experiment group B feedstuff-egg ratio comparatively test group C significantly reduces 3.90%(
p< 0.05); Between test group C and D feedstuff-egg ratio without significant difference (
p> 0.05).
Result is pointed out, the daily ration that anti-heat stress fermented feed in the embodiment of the present invention of feeding 1 and the raw material of corresponding proportion are mixed with, although daily ration metabolizable energy and protein level are lower than other each group, but the laying rate of laying hen and feedstuff-egg ratio improve the most obvious, significantly be better than the test group C of control group A and interpolation probiotics, the test group D comparatively adding GABA also has some improvement.This shows, vast scale adds the fermented feed of wet basis state, and superposition plays viable bacteria and the microecological regulation and control effect of metabolite and the central neurotransmitter regulating and controlling effect of GABA, obvious to alleviation effect of heat stress of layer.
(2) rate of broken eggs: A, B, C, D tetra-processed group laying hens test the rate of broken eggs of 4 time-of-weeks in table 4.
Table 4 anti-heat stress fermented feed affects unit to egg rate of broken eggs: %
| Time | A | B | C | D |
| 1-2 week | 2.67 a | 2.03 b | 2.61 a | 2.55 a |
| 3-4 week | 3.48 a | 2.15 b | 3.08 a | 2.44 b |
| 1-4 week | 3.08 a | 2.09 c | 2.85 ab | 2.50 abc |
The rate of broken eggs result display of different disposal group: test 1-2 is all, 3-4 is all, all minimum with the rate of broken eggs of B group egg, and rate of broken eggs is D, C, A group from low to high successively; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D rate of broken eggs reduce 32.03%(respectively
p< 0.05), 7.48%(
p> 0.05), 18.86%(
p> 0.05); Experiment group B rate of broken eggs comparatively test group C significantly reduces 26.54%(
p< 0.05); Between test group C and D rate of broken eggs without significant difference (
p> 0.05).
Result is pointed out, vast scale of feeding adds the experiment group B of wet basis state fermented feed, superposition plays viable bacteria and the microecological regulation and control effect of metabolite and the central neurotransmitter regulating and controlling effect of GABA, calcium when effectively preventing heat stress of layer, phosphorus absorb and decline and adverse effect that the deposition of trace elements such as eggshell calcium, phosphorus and manganese reduces, minimizing rate of broken eggs.
(3) antioxygenic property: A, B, C, D tetra-processed group laying hens at the Antioxidant Indexes of test after 4 weeks in table 5.
Table 5 anti-heat stress fermented feed is on the impact of laying hen antioxygenic property
| Index | A | B | C | D |
| MDA | 3.07 a | 2.45 b | 2.88 a | 2.71 ab |
| SOD | 81.0 a | 88.2 b | 84.4 a | 82.3 a |
| GSH-PX | 2462.3 a | 2717.9 b | 2583.6 a | 2501.9 a |
The body Antioxidant Indexes result display of different disposal group: tested for 4 weekends, Serum MDA (MDA) content is minimum with experiment group B, and MDA content is D, C, A group from low to high successively; Compared with control group A, experiment group B, C, D serum MDA reduce 20.20%(respectively
p< 0.05), 6.19%(
p> 0.05), 11.73%(
p> 0.05); Experiment group B serum MDA comparatively test group C significantly reduces 14.93%(
p< 0.05); Between test group C and D MDA without significant difference (
p> 0.05).
Tested for 4 weekends, superoxide dismutase (SOD) content is the highest with experiment group B, and SOD content is C, D, A group from high to low successively; Compared with control group A, experiment group B, C, D SOD in serum improve 8.89%(respectively
p< 0.05), 4.20%(
p> 0.05), 1.60%(
p> 0.05); Experiment group B SOD in serum comparatively test group C, D improves 4.50% and 7.17% respectively, difference all significantly (
p< 0.05).
Tested for 4 weekends, glutathione peroxidase (GSH-PX) content is the highest with experiment group B, and GSH-PX content is C, D, A group from high to low successively; Compared with control group A, experiment group B, C, D serum GSH-PX improve 10.38%(respectively
p< 0.05), 4.93%(
p> 0.05), 1.61%(
p> 0.05); Experiment group B serum GSH-PX comparatively test group C, D improves 5.20% and 8.63% respectively, difference all reach the level of signifiance (
p< 0.05).
Result is pointed out, the daily ration that anti-heat stress fermented feed in the embodiment of the present invention of feeding 1 and the raw material of corresponding proportion are mixed with, although daily ration metabolizable energy and protein level are lower than other each group, but Serum MDA (MDA) content is significantly lower than the test group D of control group A, the test group C adding probiotics, interpolation GABA, and superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) are significantly higher than A, C, D group.This shows, not mycetome metabolite few additive probiotics, add single central neurotransmitter GABA to improving the antioxidation of body and not obvious, and with the wet basis state fermented feed that 10% vast scale adds, play the microecological regulation and control of viable bacteria and metabolite and the dual superposition of central neurotransmitter regulation and control, laying hen oxidation resistance significantly strengthens, and then anti-heat stress effect also significantly improves.
Last it is noted that above embodiment only in order to illustrate the present invention and and unrestricted technical scheme described in the invention; Therefore, although this description with reference to each above-mentioned embodiment to present invention has been detailed description, those of ordinary skill in the art should be appreciated that and still can modify to the present invention or equivalent to replace; And all do not depart from technical scheme and the improvement thereof of the spirit and scope of the present invention, it all should be encompassed in right of the present invention.
Claims (6)
1. a laying hen anti-heat stress fermented feed, it is characterized in that, by gross weight 1000 parts, comprise following raw material: corn 50 ~ 200 parts, dregs of beans 50 ~ 150 parts, Cottonseed Meal 50 ~ 200 parts, pomace 30 ~ 100 parts, chili meal 0 ~ 100 part, whitewashing maize peel 30 ~ 120 parts, wheat bran 100 ~ 250 parts, bacterium liquid 30 ~ 50 parts, compound premixed feed 15 ~ 25 parts, 200 ~ 300 parts, water;
By gross weight 1000 parts, consisting of of described bacterium liquid: enterococcus faecalis F6 5 ~ 15 parts, bacillus subtilis 5 ~ 10 parts, S. cervisiae 5 ~ 10 parts, 50 ~ 100 parts, brown sugar, 865 ~ 935 parts, water.
2. laying hen according to claim 1 anti-heat stress fermented feed, it is characterized in that, by gross weight 1000 parts, consisting of of described compound premixed feed: trace element premixing feed 60 ~ 100 parts, vitamin premixed feed 10 ~ 18 parts, stone flour 200 ~ 300 parts, calcium monohydrogen phosphate 400 ~ 500 parts, complex enzyme 10 ~ 15 parts, phytase 5 ~ 10 parts, containing the L lysine HCL 20 ~ 40 parts of lysine 78%, DL-METHIONINE 20 ~ 80 parts, threonine 20 ~ 40 parts, salt 30 ~ 50 parts, containing the Choline Chloride 10 ~ 20 parts of choline 50%, ethoxyquinoline 0.5 ~ 1.0 part, powdered rice hulls 0 ~ 10 part, zeolite powder 0 ~ 50 part.
3. laying hen according to claim 2 anti-heat stress fermented feed, it is characterized in that, by gross weight 1000 parts, consisting of of described trace element premixing feed: ferrous sulfate monohydrate 140 ~ 200 parts, cupric sulfate pentahydrate 15 ~ 50 parts, monohydrate zinc sulphate 120 ~ 170 parts, manganese sulfate monohydrate 140 ~ 180 parts, a water sodium selenite 15 ~ 25 parts, calcium iodate 15 ~ 30 parts, cobalt chloride 5.0 ~ 30 parts, ethoxyquinoline 0.5 ~ 2.0 part, zeolite powder 313.0 ~ 544.5 parts.
4. laying hen according to claim 2 anti-heat stress fermented feed, is characterized in that, by gross weight 1000 parts, and consisting of of described vitamin premixed feed: retinyl acetate 60 ~ 80 parts, vitamin D
312 ~ 30 parts, vitamin E 100 ~ 200 parts, menadione sodium bisulfite 5.0 ~ 15 parts, thiamine hydrochloride 7.0 ~ 10 parts, Riboflavin Tetrabutyrate 5 ~ 45 parts, puridoxine hydrochloride 15 ~ 20 parts, Cobastab
125.0 ~ 8.0 parts, D-VB5 calcium 30 ~ 55 parts, niacinamide 90 ~ 130 parts, 3.5 ~ 6.0 parts, folic acid, Bio 25 ~ 35 parts, ethoxyquinoline 0.5 ~ 2.0 part, powdered rice hulls 364.0 ~ 622.0 parts.
5. laying hen according to claim 2 anti-heat stress fermented feed, it is characterized in that, by gross weight 1000 parts, consisting of of described complex enzyme: cellulase 100 parts, zytase 500 parts, acid protease 200 parts, AMS 100 parts, powdered rice hulls 100 parts.
6. the preparation method of anti-heat stress fermented feed of laying hen described in claim 1, it is characterized in that comprising the following steps: (1) raw material is pulverized: by other raw materials beyond bacteria-removing liquid, compound premixed feed and water in raw material through weighing, measuring, feed intake, promote, pulverize after removal of impurities, the raw material after pulverizing is by the sieve aperture of diameter 1.2 ~ 2.5mm; (2) siccative mixing: by the raw material after pulverizing and compound premixed feed Homogeneous phase mixing, obtain mixed material; (3) bacterium solution preparation: enterococcus faecalis F6, bacillus subtilis, S. cervisiae and brown sugar, water Homogeneous phase mixing are made bacterium liquid, keeps 35 ~ 45 DEG C of water temperature 0.5 ~ 1.0 h, obtains the bacterium liquid activated; (4) secondary wet feed mixing: by bacterium liquid, water, the mixed material Homogeneous phase mixing of activation, obtain mixed material; (5) anaerobic fermentation: by mixed material anaerobic fermentation 5 ~ 8 days at 18 ~ 45 DEG C, obtain anti-heat stress fermented feed.
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