CN114145384B - Fermentation method for increasing lysine content of traditional Chinese medicine feed additive - Google Patents
Fermentation method for increasing lysine content of traditional Chinese medicine feed additive Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 77
- 239000004472 Lysine Substances 0.000 title claims abstract description 36
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- 235000015099 wheat brans Nutrition 0.000 claims abstract description 9
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a solid state fermentation method for increasing lysine content, and belongs to the technical field of microbial fermentation. Through special culture medium and culture conditions, magnolia powder, hawthorn powder, epimedium powder, bentonite, medical stone and wheat bran are fermented, and finally, the solid-state fermented feed with high lysine content can be obtained, and the daily gain rate can be obviously increased after the feed is eaten by birds, thus laying a foundation for high-lysine solid fermentation.
Description
Technical Field
The invention belongs to the technical field of microbial fermentation, and particularly relates to a solid state fermentation method for increasing lysine content.
Background
The Chinese herbal medicine is taken as a feed additive or mixed feeding medicament, is an important aspect of veterinary Chinese herbal medicine application in recent years, and is a unique series of feed additives. Because the traditional Chinese medicine belongs to pure nature, has rich resources, is easy to obtain, has small toxic and side effects, has no harmful residues in animal bodies and meat, eggs and milk, and can create green and safe food for people.
The fermentation of the traditional Chinese medicine can change the dissolution efficiency of the medicine, improve the palatability of the traditional Chinese medicine, and further strengthen the effect of the medicine due to the synergistic effect of probiotics and the traditional Chinese medicine.
The efficacy is exerted on the premise of nutrition and health of animals. The traditional Chinese medicine fermented feed additive is based on full nutrition supply to strengthen absorption, prevent diseases and promote growth and quality improvement.
The fermentation of many traditional Chinese medicine feed additives on the market only focuses on the smell of traditional Chinese medicines, the number of probiotics, the dissolution efficiency of active ingredients and the like. In fact, the increase of limiting amino acids, such as lysine, during fermentation is also of great importance.
The content of lysine in the traditional Chinese medicine fermented feed additive is increased, so that the lysine and the effective components in the traditional Chinese medicine can cooperatively play a role, the nutrition absorption efficiency is improved, and the drug effect of the traditional Chinese medicine is amplified.
The invention improves the lysine content in the fermentation process of the traditional Chinese medicine feed additive through a novel process, thereby providing a foundation for full nutrition supply.
Therefore, how to provide a solid state fermentation method for increasing lysine content is a problem to be solved in the art.
Disclosure of Invention
The invention discloses a solid state fermentation method for increasing lysine content.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a solid state fermentation process for increasing lysine content comprising the steps of:
(1) Mixing of powder
Mixing the primary materials of the traditional Chinese medicines: according to mass, magnolia bark powder, hawthorn powder, epimedium powder and glossy privet fruit powder are mixed according to the mass ratio of 5-6:1-2:2-2.5:2-4 percent of the raw materials, then adding glucose accounting for 4 percent of the mass of the raw materials, 1 percent of peptone accounting for 1 percent of the raw materials, and 40 percent of soybean meal accounting for 40 percent of the raw materials to obtain the raw materials;
traditional Chinese medicine enzyme mixture: adding cellulase accounting for 0.2 percent of the mass of the traditional Chinese medicine enzyme mixture into the traditional Chinese medicine mixture, adding lignin enzyme accounting for 0.3 percent of the mass of the traditional Chinese medicine enzyme mixture, and uniformly mixing to obtain the traditional Chinese medicine enzyme mixture
Fermenting the whole material: adding bentonite accounting for 0.1 percent of the mass of the traditional Chinese medicine enzyme mixture and medical stone accounting for 0.12 percent of the mass of the traditional Chinese medicine enzyme mixture into the traditional Chinese medicine enzyme mixture, and uniformly mixing wheat bran accounting for 8 percent of the mass of the traditional Chinese medicine enzyme mixture to obtain a fermented whole material;
preferably, the medical stone and the wheat bran are crushed and pass through a 80-mesh sieve;
(2) Solid state fermentation
Adding 5% lactobacillus plantarum bacterial solution and 3% clostridium butyricum bacterial solution into 100kg of traditional Chinese medicine mixed primary material, adding 35kg of sterilized tap water, and uniformly mixing to obtain a bottom fermentation material;
200kg of traditional Chinese medicine enzyme mixture is added with 10% of saccharomycete liquid, and the concentration of saccharomycete is about 4.5 multiplied by 10 7 Adding 28% sterilized tap water into the mixture per mL, and uniformly mixing the mixture to obtain a middle layer material for fermentation;
600kg of fermentation complete material, 10% of saccharomycete liquid, 5% of bacillus subtilis liquid and 28% of sterilized tap water are added, and the materials are uniformly mixed to be used as upper material;
primary fermentation: after stacking, fermenting for 2d at the temperature of 32 ℃ to obtain a first fermentation product;
and (3) secondary fermentation: uniformly mixing the first fermentation products, fermenting for 1d at 30 ℃, and fermenting the materials in a fermentation tank;
and (3) fermenting for the third time: filling the fermentation materials in the fermentation tank into breathing bags, each bag containing 15kg, sealing the sealing of the fermentation breathing bags after bagging, and fermenting for 5 days at 30 ℃ to obtain a fermentation finished product.
Preferably, the preparation method of the bacterial liquid comprises the following steps:
scraping tricyclobacteria from the yeast strain preserved on the inclined plane by using an inoculating loop (the diameter of the loop is 0.3 mm), inoculating 300mL of activating culture medium, loading 500mL of triangular flask activating culture medium, culturing at 33 ℃ at the rotating speed of 180r/m, and culturing the yeast for 18h to obtain a yeast liquid;
scraping pentacyclic bacteria from the inclined plane preserved lactobacillus plantarum by an inoculating loop (loop diameter is 0.3 mm), inoculating 300mL of activating culture medium, loading into a 500mL triangular flask, culturing at 33 ℃ at a rotating speed of 180r/m, and culturing saccharomycetes for 18h to obtain lactobacillus plantarum liquid;
scraping out the bicyclic bacteria from the bacillus subtilis preserved on the inclined plane by using an inoculating loop (the diameter of the loop is 0.3 mm), inoculating 300mL of activating culture medium, filling into a 500mL triangular flask, culturing at 33 ℃ at the rotating speed of 180r/m, and culturing saccharomycetes for 18 hours to obtain bacillus subtilis liquid;
scraping off the hexacyclic bacteria from the clostridium butyricum preserved on the inclined plane by using an inoculating loop (the diameter of the loop is 0.3 mm), inoculating 300mL of activating culture medium, filling into a 500mL triangular flask, culturing at 33 ℃ at the rotating speed of 180r/m, and culturing saccharomycetes for 22 hours to obtain clostridium butyricum liquid;
the strain activation culture medium comprises the following components: yeast extract 1% (w/w), peptone 1% (w/w), glucose 4% (w/w);
adding 5% lactobacillus plantarum bacterial solution and 3% clostridium butyricum bacterial solution into 100kg of traditional Chinese medicine mixed primary materials, adding 35kg of sterilized tap water, uniformly mixing, and uniformly spreading at the bottom of a fermentation tank to obtain a bottom fermentation material;
200Kg of traditional Chinese medicine enzyme mixture is added with 10% of saccharomycete liquid, added with 28% of sterilized tap water, uniformly mixed, and evenly spread on the bottom fermentation material as a middle layer material for fermentation;
600kg of fermented complete material, 10% of saccharomycete liquid, 5% of bacillus subtilis liquid and 28% of sterilized tap water are added, the materials are uniformly mixed to form an upper material, and the upper material is uniformly paved on the upper part of the middle material;
the fermenter was placed in a sterile fermenter at 32℃for 2d. Pouring out the fermentation materials in the fermentation tank, stirring the three layers of materials, uniformly mixing, then completely filling the materials into the fermentation tank, and fermenting for 1d in a sterile fermentation room with the temperature of 30 ℃ to obtain the fermentation tank fermentation materials.
The fermenter was filled with a breathing bag (25 kg,51×81×10 filaments, wenzhou wound packing material limited) of 15kg each, and heat sealed with a plastic sealer (plastic bag sealer, dongguan Xiang mechanical and electrical equipments limited). Fermenting for 5 days at room temperature of 30deg.C to obtain fermented product;
the application of the solid state fermentation method in preparing the feed with high lysine content;
the application of the solid state fermentation method in preparing poultry feed with high lysine content;
the application of the state fermentation method in preparing the poultry weight-gaining feed.
In summary, the invention discloses a solid state fermentation method for increasing the content of lysine, which is characterized in that magnolia powder, hawthorn powder, epimedium powder, bentonite, medical stone and wheat bran are fermented by a special culture medium and culture conditions, so that a solid state fermentation feed with high content of lysine can be finally obtained, the daily gain rate can be obviously increased after the feed is eaten by birds, and a foundation is laid for high-lysine solid state fermentation.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Bentonite: shijia guangning mineral products limited; medical stone: mineral processing plant of Lingshu county Jiang Feng; bean pulp: shandong Fu Li Heng New Material technologies Co., ltd; cortex Magnoliae officinalis: bozhou Hongbaotang commercial and trade company limited; hawthorn fruit: qingzhou Dafu agriculture development Co.Ltd; epimedium herb: bailin, bozhou, inc.; fructus ligustri lucidi: bozhou benevolence chinese herbal medicine sales limited; wheat bran: jining is a rich company of pastoral, inc.; cellulase cellulose CEL-01 type: xia Cheng group; ligninase 1000U Industrial grade: shandong Nojie Biotechnology Co., ltd.
Saccharomyces cerevisiae, bacillus subtilis, purchased from all companies under the state of the Wang generator technology research; clostridium butyricum is purchased from henna and Yangshao biotechnology limited company in Henan; lactobacillus plantarum is purchased from Weifang Rui Chen Biotechnology Inc.
Example 1
Crushing
Pulverizing soybean meal, cortex Magnolia officinalis, fructus crataegi, herba Epimedii, and fructus Ligustri Lucidi with water drop type pulverizer, sieving with 40 mesh sieve to obtain soybean meal powder, cortex Magnolia officinalis powder, fructus crataegi powder, herba Epimedii powder, and fructus Ligustri Lucidi powder.
Bacterial activation and expansion culture
Strain activation medium: yeast extract 1% (w/w), peptone 1% (w/w), glucose 4% (w/w);
strain expansion culture medium: yeast powder 0.2% (w/w), peptone 0.5% (w/w), glucose 6% (w/w);
inoculating lactobacillus plantarum into an activation culture medium, inoculating into a 500mL triangular flask, loading 300mL of the activation culture medium, culturing at 33 ℃ at a rotating speed of 180r/m, and culturing saccharomycetes for 18 hours to obtain lactobacillus plantarum liquid;
inoculating bacillus subtilis strain into an activation culture medium, inoculating 500mL triangular flask, loading 300mL of activation culture medium, culturing at 33 ℃ at a rotation speed of 180r/m, and culturing saccharomycetes for 18h to obtain bacillus subtilis liquid;
inoculating clostridium butyricum into an activation culture medium, inoculating 500mL triangular flask, loading 300mL of activation culture medium, culturing at 33 ℃ at a rotation speed of 180r/m, and culturing yeast for 22h to obtain clostridium butyricum liquid;
wherein the triangular bottle such as lactobacillus plantarum, clostridium butyricum and the like wraps the bottle mouth by a multilayer preservative film, and then the preservative film is wound by a transparent adhesive tape to ensure no air leakage or air intake.
Powder mixing
The mixture is prepared from magnolia bark powder, hawthorn powder, epimedium powder and glossy privet fruit powder=5: 2:2.5:4, mixing the materials in proportion, and crushing the materials after mixing to obtain the traditional Chinese medicine mixed dry powder. Adding glucose 4% by mass, peptone 1% by mass and soybean meal 40% by mass into the traditional Chinese medicine mixed dry powder to obtain a traditional Chinese medicine mixed primary material.
Adding cellulase 0.2% and ligninase 0.3% into the Chinese medicinal mixture, and mixing to obtain Chinese medicinal enzyme mixture
The traditional Chinese medicine enzyme mixture is added with 0.1 percent of bentonite, 0.12 percent of medical stone and 8 percent of wheat bran, and the mixture is uniformly mixed to obtain the fermented whole material.
Solid state fermentation
A stainless steel tank (4 mx2mx1 m) was used as a fermentation tank without covering.
100kg of traditional Chinese medicine mixed primary material is added with lactobacillus plantarum bacterial liquid with the mass of 5% and clostridium butyricum bacterial liquid with the mass of 3%, 35kg of sterilized tap water is added, and the mixture is uniformly mixed and evenly spread at the bottom of a fermentation tank to obtain a bottom fermentation material.
200kg of traditional Chinese medicine enzyme mixture is added with 10% of saccharomycete liquid, added with 28% of sterilized tap water, evenly mixed, and evenly spread on the bottom fermentation material as a middle layer material for fermentation.
600kg of fermented complete material, 10% of saccharomycete liquid, 5% of bacillus subtilis liquid and 28% of sterilized tap water are added, and the materials are uniformly mixed to form an upper material, and then the upper material is uniformly paved on the upper part of the middle material.
The fermenter was placed in a sterile fermenter at 32℃for 2d. Pouring out the fermentation materials in the fermentation tank, stirring the three layers of materials, uniformly mixing, then completely filling the materials into the fermentation tank, and fermenting for 1d in a sterile fermentation room with the temperature of 30 ℃ to obtain the fermentation tank fermentation materials.
The fermenter was filled with a breathing bag (25 kg,51×81×10 filaments, wenzhou wound packing material limited) of 15kg each, and heat sealed with a plastic sealer (plastic bag sealer, dongguan Xiang mechanical and electrical equipments limited). Fermenting for 5 days at room temperature of 30 ℃ to obtain a fermented finished product.
Result detection
The detection of the heavy lysine content of the fermented finished product is carried out by adopting a literature method (Fang Hua, spring source, silver red silk. Optimization of fermentation conditions of high-lysine fermented soybean meal, grain and feed industry, 2008,3), and the lysine content in the fermented finished product is measured to be 0.141%.
The fermented product was measured for moisture with a rapid moisture meter and then added to the broiler feed base ration at an add-on level of 0.2% of dry matter. After 22-day-old broilers purchased in the AA broiler market are fed every day, the weight of feed fed by the broilers is calculated, and according to the experimental method and the analysis method of literature (Zhang Xiaoyi, guan Guzhen, wei Xiaochen, lutianjiao, li Jianuo, chen Xiaofeng. Influence of composite traditional Chinese medicine fermentation products on the growth performance, oxidation resistance and immunity performance of the broilers, poultry nutrition and 2012), the experiment and the analysis are carried out, and the feed weight ratio and the daily gain are determined.
The determination shows that the daily gain of the broiler chickens is 67.9g without adding fermentation products for feeding, and the feed weight ratio is 1.91; after the fermentation product is added for feeding, the daily gain of the broiler chickens is 75.3g, and the feed weight ratio is 1.80
Example 2
The powder mixing procedure was as follows, the other operations being the same as in example 1:
the mixture is prepared from magnolia bark powder, hawthorn powder, epimedium powder and glossy privet fruit powder=5: 2:2:2, mixing the materials in proportion, and crushing the materials after mixing to obtain the traditional Chinese medicine mixed dry powder. Adding glucose 4% by mass, peptone 1% by mass and soybean meal 40% by mass into the traditional Chinese medicine mixed dry powder to obtain a traditional Chinese medicine mixed primary material.
Adding cellulase 0.2% and ligninase 0.3% into the Chinese medicinal mixture, and mixing to obtain Chinese medicinal enzyme mixture
The traditional Chinese medicine enzyme mixture is added with 0.1 percent of bentonite, 0.12 percent of medical stone and 8 percent of wheat bran, and the mixture is uniformly mixed to obtain the fermented whole material.
Result detection
The detection of the heavy lysine content of the fermented finished product is carried out by adopting a literature method (Fang Hua, spring source, silver red silk. Optimization of fermentation conditions of high-lysine fermented soybean meal, grain and feed industry, 2008,3), and the lysine content in the fermented finished product is measured to be 0.136%.
The fermented product was measured for moisture with a rapid moisture meter and then added to the broiler feed base ration at an add-on level of 0.2% of dry matter. After 22-day-old broilers purchased in the AA broiler market are fed every day, the weight of feed fed by the broilers is calculated, and according to the experimental method and the analysis method of literature (Zhang Xiaoyi, guan Guzhen, wei Xiaochen, lutianjiao, li Jianuo, chen Xiaofeng. Influence of composite traditional Chinese medicine fermentation products on the growth performance, oxidation resistance and immunity performance of the broilers, poultry nutrition and 2012), the experiment and the analysis are carried out, and the feed weight ratio and the daily gain are determined.
The determination shows that the daily gain of the broiler chickens is 67.9g without adding fermentation products for feeding, and the feed weight ratio is 1.91; after the fermentation product is added for feeding, the daily gain of the broiler chickens is 75.1g, and the feed weight ratio is 1.82
Example 3
The powder mixing procedure was as follows, the other operations being the same as in example 1:
the mixture is prepared from magnolia bark powder, hawthorn powder, epimedium powder and glossy privet fruit powder=6: 2:2:2, mixing the materials in proportion, and crushing the materials after mixing to obtain the traditional Chinese medicine mixed dry powder. Adding glucose 4% by mass, peptone 1% by mass and soybean meal 40% by mass into the traditional Chinese medicine mixed dry powder to obtain a traditional Chinese medicine mixed primary material.
Adding cellulase 0.2% and ligninase 0.3% into the Chinese medicinal mixture, and mixing to obtain Chinese medicinal enzyme mixture
The traditional Chinese medicine enzyme mixture is added with 0.1 percent of bentonite, 0.12 percent of medical stone and 8 percent of wheat bran, and the mixture is uniformly mixed to obtain the fermented whole material.
Result detection
The detection of the heavy lysine content of the fermented finished product is carried out by adopting a literature method (Fang Hua, spring source, silver red silk. Optimization of fermentation conditions of high-lysine fermented soybean meal, grain and feed industry, 2008,3), and the lysine content in the fermented finished product is measured to be 0.131%.
The fermented product was measured for moisture with a rapid moisture meter and then added to the broiler feed base ration at an add-on level of 0.2% of dry matter. After 22-day-old broilers purchased in the AA broiler market are fed every day, the weight of feed fed by the broilers is calculated, and according to the experimental method and the analysis method of literature (Zhang Xiaoyi, guan Guzhen, wei Xiaochen, lutianjiao, li Jianuo, chen Xiaofeng. Influence of composite traditional Chinese medicine fermentation products on the growth performance, oxidation resistance and immunity performance of the broilers, poultry nutrition and 2012), the experiment and the analysis are carried out, and the feed weight ratio and the daily gain are determined.
The determination shows that the daily gain of the broiler chickens is 67.9g without adding fermentation products for feeding, and the feed weight ratio is 1.91; after the fermentation product is added for feeding, the daily gain of the broiler chickens is 75.1g, and the feed weight ratio is 1.81
Comparative example 1
Except for the bottom material, the middle material, the upper building and the like, the mixture is uniformly mixed and filled into a fermentation tank, the lysine content in the fermented finished product is 0.102% as measured in the same example 1, and after the fermented product is added for feeding, the daily gain of the broiler chickens is 74.5g, and the feed weight ratio is 1.85.
Comparative example 2
Except that bentonite and medical stone are not added in the whole fermentation material, the lysine content in the finished fermentation product is 0.10% as measured in the example 1, and the daily gain of the broiler chickens is 74.7g and the weight ratio is 1.86 after the fermented product is added for feeding.
Comparative example 3
Except that the traditional Chinese medicine enzyme mixture is not added with lignin enzyme, the lysine content in the fermented finished product is measured to be 0.110% in the same way as in example 1, and after the fermented product is added for feeding, the daily gain of broiler chickens is 74.7g, and the feed weight ratio is 1.85.
Comparative example 4
The results of example 1 were the same except that the three fermented materials were directly mixed and fed into a breather bag for fermentation after fermentation in a fermenter for two days, the lysine content in the fermented product was 0.113%, the daily gain of broiler chickens was 74.6g after feeding with the fermented product, and the feed weight ratio was 1.85
Comparative example 5
Except that 3% clostridium butyricum bacteria liquid is not added when the bottom layer fermentation material is prepared, 21% tap water is added, the lysine content in the fermentation finished product is measured to be 0.120% in the same example 1, the daily gain of broiler chickens is 74.6g after the fermentation product is added for feeding, and the feed weight ratio is 1.85
Comparative example 6
Adding 2% lactobacillus plantarum bacterial solution into the upper layer material, and measuring the lysine content in the fermented product to be 0.121% in the same way as in example 1, wherein the daily gain of the broiler chicken is 75.0g and the feed weight ratio is 1.82 after adding the fermented product for feeding
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the embodiments described above will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (4)
1. A solid state fermentation process for increasing lysine content comprising the steps of:
(1) Mixing raw materials of the traditional Chinese medicine powder: according to mass, magnolia bark powder, hawthorn powder, epimedium powder and glossy privet fruit powder are mixed according to the mass ratio of 5-6:1-2:2-2.5: mixing 2-4% of the raw materials, adding glucose accounting for 4% of the mass of the raw materials, adding peptone accounting for 1% of the mass of the raw materials, and adding soybean meal accounting for 40% of the mass of the raw materials to obtain a raw material; traditional Chinese medicine enzyme mixture: adding cellulase accounting for 0.2% of the mass of the traditional Chinese medicine mixed primary material into the traditional Chinese medicine mixed primary material, adding lignin enzyme accounting for 0.3% of the mass of the traditional Chinese medicine mixed primary material, and uniformly mixing to obtain a traditional Chinese medicine enzyme mixed material fermented complete material: adding bentonite with the mass of 0.1% of the traditional Chinese medicine enzyme mixture and medical stone with the mass of 0.12% of the traditional Chinese medicine enzyme mixture into the traditional Chinese medicine enzyme mixture, and uniformly mixing wheat bran with the mass of 8% of the traditional Chinese medicine enzyme mixture to obtain a fermented whole material;
(2) Solid state fermentation
Adding 5% lactobacillus plantarum bacterial solution and 3% clostridium butyricum bacterial solution into 100kg of traditional Chinese medicine mixed primary material, adding 35kg of sterilized tap water, and uniformly mixing to obtain a bottom fermentation material; 200kg of traditional Chinese medicine enzyme mixture is added with 10% of saccharomycete liquid, added with 28% of sterilized tap water and uniformly mixed to be used as a middle layer material for fermentation; 600kg of fermentation complete material, 10% of saccharomycete liquid, 5% of bacillus subtilis liquid and 28% of sterilized tap water are added, and the materials are uniformly mixed to be used as upper material;
primary fermentation: after stacking, fermenting for 2d at the temperature of 32 ℃ to obtain a first fermentation product;
and (3) secondary fermentation: uniformly mixing the first fermentation product, and fermenting for 1d at 30 ℃ to obtain a fermentation tank fermentation material;
and (3) fermenting for the third time: filling fermentation materials in a fermentation tank into breathing bags, filling 15kg of the fermentation materials in each bag, sealing the sealing of the fermentation breathing bags, and fermenting for 5 days at 30 ℃ to obtain a fermentation finished product;
the method for activating the bacterial liquid comprises the following steps:
inoculating yeast strain into an activation culture medium, bottling 300mL of the activation culture medium with 500mL triangular flask, culturing at 33 ℃ at a rotation speed of 180r/m, and culturing for 18h to obtain yeast solution;
inoculating lactobacillus plantarum into an activation culture medium, bottling 300mL of the activation culture medium with 500mL triangular bottles, culturing at 33 ℃ at a rotating speed of 180r/m, and culturing saccharomycetes for 18 hours to obtain lactobacillus plantarum liquid;
inoculating bacillus subtilis strain into an activation culture medium, bottling 300mL of the activation culture medium with 500mL triangular flask, culturing at 33 ℃ at a rotating speed of 180r/m, and culturing saccharomycetes for 18h to obtain bacillus subtilis liquid;
inoculating clostridium butyricum into an activation culture medium, bottling 300mL of activation culture medium with 500mL triangular flask, culturing at 33 ℃ at a rotating speed of 180r/m, and culturing saccharomycetes for 22h to obtain clostridium butyricum liquid; the strain activation culture medium comprises the following components: yeast extract 1% (w/w), peptone 1% (w/w), glucose 4% (w/w).
2. Use of the solid state fermentation process of claim 1 for the preparation of a feed with a high lysine content.
3. Use of the solid state fermentation process of claim 1 for the preparation of a high lysine content poultry feed.
4. Use of the method of claim 1 for the preparation of a feed for poultry weight gain.
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