CN105724772A - Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof - Google Patents

Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof Download PDF

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Publication number
CN105724772A
CN105724772A CN201610101052.7A CN201610101052A CN105724772A CN 105724772 A CN105724772 A CN 105724772A CN 201610101052 A CN201610101052 A CN 201610101052A CN 105724772 A CN105724772 A CN 105724772A
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mass parts
chinese herbal
herbal medicine
feed additive
add
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王林
周玉武
王涛
王莹
冉蓉
杨建朋
杨艳艳
张敏
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JINAN YIMIN ANIMAL MEDICINE CO Ltd
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JINAN YIMIN ANIMAL MEDICINE CO Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to the technical field of feed additives, in particular to a Chinese herbal medicine micro-ecological compound feed additive and a preparation and use method thereof. The additive comprises a Chinese herbal medicine mixture, probiotics and an enzyme preparation, the Chinese herbal medicine mixture is fermented and converted by means of probiotics, the bioavailability of active components in traditional Chinese medicine materials is improved, probiotics is reinforced through prebiotics and other nutritional matter in Chinese herbal medicine, activity is high, and stability is high. The additive is safe, environmentally friendly and free of toxin, has the functions of remarkably promoting animal growth and enhancing animal immunity, and can completely replace antibiotics to be added into feed.

Description

A kind of Chinese herbal medicine micro-ecological composite feed additive and preparation and application thereof
Technical field
The present invention relates to field of feed additive technology, particularly to a kind of Chinese herbal medicine micro-ecological composite feed additive and preparation and application thereof.
Background technology
Feed additive is requisite raw material in modern feedstuff and cultivation industry, to strengthening normal feedstuff nutritive value, improves breeding performonce fo animals, it is ensured that animal health, saves feed cost, and improving the aspects such as livestock products quality has obvious effect.In early days in the production of feed additive, the antibiotic of people's low dosage or sulfa drugs prevent disease or the diarrhoea of poultry, promote growth of animal.The antibiotic of this low dosage remains in a large number in animal body and then enters in human body, and is drained in river, soil by animal wastes etc., causes that the antibiotic such as river exceed standard, causes the appearance of pathogenic bacteria of drug-resistant, superbacteria.People recognize the harm of abuse of antibiotics gradually, and the increasing countries and regions including China at present start to forbid interpolation antibiotic in feedstuff.In order to solve this problem, people continually develop novel feed additive and carry out substitute antibiotics.
Chinese herbal feed additive is the feed additive made for raw material with Chinese herbal medicine, Chinese herbal medicine is medicine and natural product, containing plurality of active ingredients, China's application Chinese herbal medicine has a long history as feed additive, began to for promoting growth of animal before more than 2,000 years, increase weight and disease preventing and treating.
Microbial ecological agent is a kind of replacement or the viable microbial feed additive of one or more fungus strains in balance animal ecosystem, by improving intestinal microbial population balance, animal can be applied Beneficial Effect, it can excite own beneficial culture propagation to increase, and resists harmful fungus strain growth simultaneously.
Both is currently used more generally acknowledged relatively safety, green feed additive, but they also exist, and action effect is notable, it is slow to take effect, utilization rate is low, high in cost of production limitation.
Summary of the invention
In order to solve problem of the prior art, the invention provides a kind of Chinese herbal medicine micro-ecological composite feed additive and preparation and application thereof, its effect is notable, instant effect, utilization rate are high, cost is low.
The technical solution adopted in the present invention is as follows:
nullA kind of Chinese herbal medicine micro-ecological composite feed additive,Including mixture of Chinese herbal medicines、Probiotic bacteria、Enzyme preparation,Described mixture of Chinese herbal medicines consists of: Fructus Crataegi 10~20 mass parts、Radix Glycyrrhizae 10~20 mass parts、Fructus Hordei Germinatus 10~20 mass parts、The Radix Astragali 5~15 mass parts、The Rhizoma Atractylodis Macrocephalae 5~15 mass parts、Rhizoma Atractylodis 5~10 mass parts、Rhizoma Dioscoreae 0~10 mass parts、Radix Codonopsis 0~10 mass parts、Fructus Psoraleae 0~10 mass parts、Rhizoma Polygonati 0~10 mass parts、Folium Eucommiae 0~10 mass parts、Cortex Magnoliae Officinalis 0~10 mass parts、Radix Puerariae 0~10 mass parts、Semen Coicis 0~10 mass parts、Fructus Schisandrae Chinensis 0~10 mass parts、Poria 0~5 mass parts、Fructus Lycii 0~5 mass parts、Radix Rhodiolae 0~5 mass parts、Flos Lonicerae 0~5 mass parts、Pericarpium Citri tangerinae 0~5 mass parts、Flower of poplar 0~5 mass parts、Fructus Aurantii 0~5 mass parts、Pericarpium Citri Reticulatae 0~5 mass parts.
Probiotic bacteria is bacillus subtilis, Bacillus licheniformis, bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum.
Enzyme preparation is: the xylanase of the glucoseoxidase of 250U/g, the alpha-galactosidase of 20U/g, the 'beta '-mannase of 500U/g, the phytase of 100U/g and 500U/g.
A kind of preparation method of Chinese herbal medicine micro-ecological composite feed additive, it comprises the following steps:
(1) described mixture of Chinese herbal medicines is pulverized: weighed various Chinese crude drug, mix homogeneously by formula, be crushed to all through 20 mesh sieves;
(2) Chinese herbal medicine substrate preparation: add in the mixture of Chinese herbal medicines powder after pulverizing and account for the Semen Maydis powder of 10% weight ratio and the bean cake powder of 5% weight ratio, add the water of 1~3 times, mixing, sterilizing 20min at 121 DEG C;
(3) probiotic's culture: take frozen probiotic bacteria strain respectively, be inoculated in seed culture medium, cultivates 24h at 37 DEG C, obtains the probiotic bacteria seed liquor of bacterial content >=1 × 108/mL;
(4) Chinese herbal medicine probiotics fermention: add pre-incubated bacillus subtilis and Bacillus licheniformis seed liquor in the Chinese herbal medicine substrate after sterilizing by the inoculum concentration of 5%, after 35 DEG C~37 DEG C ferment at constant temperature cultivate 4h~12h, add the seed liquor of bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum again by the inoculum concentration of 2%, continue to terminate fermentation after ferment at constant temperature cultivates 24h~48h at 30 DEG C~35 DEG C;
(5) dry: in the fermentation culture medium that step (4) obtains, to add the Semen Maydis powder of 1~3 times of weight, after mixing through vacuum or lyophilization to moisture below 10%, be ground into powder;
(6) batch mixing: add enzyme preparation, mix homogeneously in dried fermentation culture medium, to obtain final product.
The using method of a kind of Chinese herbal medicine micro-ecological composite feed additive, is addition 0.1~1kg Chinese herbal medicine micro-ecological composite feed additive in animal and fowl fodder per ton, uses after mixing.
Technical scheme provided by the invention has the benefit that
The Chinese herbal medicine micro-ecological composite feed additive that in the present inventive method prepared by step, Chinese herbal medicine is through probiotic bacteria external degradation and conversion, the material that the animal such as cellulose, lignin is difficult to absorb is degraded to low-molecular-weight carbohydrate, produces multiclass and can promote the prebiotic agents of growth of probiotics;Active substance in medical material is able to abundant release, and the active substance of part macromole is also degraded to the small molecule active composition of easily absorption, produces more activated product and somatomedin.Probiotic bacteria then utilizes the nutritional labeling in Chinese herbal medicine, and number of viable increases substantially, and activity is greatly enhanced, and produces the various active materials such as metabolic enzymes, organic acid, somatomedin class, anti-microbial type.
From above-mentioned technical scheme it can be seen that the invention have benefit that:
(1) Chinese crude drug that the present invention uses is the Chinese crude drug of integration of edible and medicinal herbs, all list feedstuff catalogue or feed additive catalogue in, the probiotic bacteria used also is in feed additive catalogue the probiotic bacteria included, product safety is green, without any toxicity, antibiotic-free remains, and life-time service will not develop immunity to drugs;
(2) Chinese crude drug converts through probiotic bacteria, the active substance of macromole is also degraded to the small molecule active composition of easily absorption, improve the bioavailability of active component in Chinese crude drug, a small amount of interpolation can reach disease preventing and treating, constitutional effect, and use cost is low;
(3) variation of the probiotics strain in product of the present invention and be the relation of Mutualism, forms close with intestinal beneficial bacterium group, and the nutrient substance strengthening such as the prebiotics in Chinese herbal medicine, and activity is high, and stability by force, is widely used in various cultivated animals;
(4) Chinese herbal medicine and probiotic bacteria are organically combined by product of the present invention, and add the enzyme lacked in animal digestive system, and three reaches the effect of Synergistic, it is possible to be obviously enhanced animal body constitution, promote growth of animal, improve efficiency of feed utilization.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly, it is described in further detail below in conjunction with detailed description of the invention.
Embodiment one
Prepare middle medicinal herbs micro-zoology composite feed additive according to the following steps:
(1) Chinese herbal medicine is pulverized: weighed various Chinese crude drug by formula, mix homogeneously, being crushed to all through 20 mesh sieves, formula is: Fructus Crataegi 20 parts, 20 parts of Radix Glycyrrhizae, 20 parts of Fructus Hordei Germinatus, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 10 parts, Rhizoma Atractylodis 5 parts, Rhizoma Dioscoreae 5 parts, Radix Codonopsis 5 parts, Fructus Psoraleae 5 parts, Rhizoma Polygonati 10 parts, Folium Eucommiae 5 parts, Cortex Magnoliae Officinalis 5 parts, Radix Puerariae 5 parts, Semen Coicis 5 parts, Flos Lonicerae 5 parts, Pericarpium Citri tangerinae 5 parts, flower of poplar 5 parts, Fructus Aurantii 5 parts, Pericarpium Citri Reticulatae 5 parts;
(2) Chinese herbal medicine substrate preparation: add the Semen Maydis powder of 10% weight ratio and the bean cake powder of 5% weight ratio in the herbal powder after pulverizing, add the water of 1~3 times, mixing, 121 DEG C of sterilizing 20min;
(3) probiotic's culture: take frozen probiotic bacteria strain respectively, be inoculated in seed culture medium, cultivates 24h, obtains the probiotic bacteria seed liquor of bacterial content >=1 × 108/mL for 37 DEG C;
(4) Chinese herbal medicine probiotics fermention: add pre-incubated bacillus subtilis and Bacillus licheniformis seed liquor in the Chinese herbal medicine substrate after sterilizing by the inoculum concentration of 5%, after 35 DEG C of ferment at constant temperature cultivate 8h, add the seed liquor of bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum again by the inoculum concentration of 2%, 30 DEG C are continued to terminate fermentation after ferment at constant temperature cultivates 48h;
(5) dry: fermentation culture medium adds 3 times of weight Semen Maydis powder, after mixing through vacuum or lyophilization to moisture below 10%, be ground into powder;
(6) batch mixing: add enzyme preparation, mix homogeneously in dried fermentation culture medium, to obtain final product.
The present embodiment is intended to be tested by feeding of broiler, investigates the impact on feeding of broiler effect of the Traditional Chinese drug microecology feed additive.Randomly select 450 7 age in days white meat-type chickens, be divided into blank group, antibiotic group and additive group, often 5 repetitions of group, 30 chickens of each repetition.Raising 42 days, wherein 7-20 age in days basal diet is that after 511,20 ages in days, basal diet is 510.Blank group only feeds basal diet, and antibiotic group adds 60g Salinomycin in basal diet per ton, and additive group adds the Traditional Chinese drug microecology feed additive in 500g embodiment 1 in basal diet per ton.Blank group and antibiotic group carry out immunity according to program, and Traditional Chinese drug microecology feed additive group is not immune.
Blank group with antibiotic group immune programme for children is:
7 ages in days, newcastle-biography Combined vaccine, eye dripping collunarium;
The 14 poisoning vaccines of age in days fabricius bursa, eye dripping collunarium;
21 age in days avian influenza vaccine neck notes;
28 age in days newcastles IV are Seedling (Lasota strain) drinking-water.
After feeding 42 days, experimental results is as shown in table 1.
Table 1 white meat-type chickens raises result
Table 1 is not it is shown that in using embodiment 1 gained feed additive process, 150 chickens of additive group use other any medicine and vaccines in feeding, it does not have the disease such as IB and Pullorum Disease occurs, and growth of meat chicken physically well develops.The about 200g that increases weight than blank group fed 42 days by every additive group broiler, and ratio antibiotic group weightening finish 100g, growth promotion and enhancing immunity effect are obvious.
Applicating example
1, pig farm, Linyi County of Shandong Province one, randomly select 100 head growth growing and fattening pigs, full price basal diet per ton adds the Traditional Chinese drug microecology feed additive in 1000g embodiment 2, after using 50 days continuously, compared with the growing and fattening pigs not using Traditional Chinese drug microecology feed additive to feed, average weight height 10g~20kg, average head daily gain height 200g~400g, average feed-weight ratio is low by 0.5~0.8, and uses the growing and fattening pigs that Traditional Chinese drug microecology feed additive feeds the symptoms such as diarrhoea seldom occur.
2, Yinan County, Shandong Province one meat-type duck cultivation company, randomly select a cultivation greenhouse, amount to 2200 cherry valley ducks, from 1 age in days, basal diet per ton adds the Chinese herbal medicine micro-ecological composite feed additive in 300g embodiment 3, delivers for sale after feeding 40 days, compared with the cultivation greenhouse not using Traditional Chinese drug microecology feed additive to feed, average weight height 100g~300g, marketing time shifts to an earlier date 5~10 days.
Embodiment two
Prepare middle medicinal herbs micro-zoology composite feed additive according to the following steps:
(1) Chinese herbal medicine is pulverized: weighed various Chinese crude drug by formula, mix homogeneously, being crushed to all through 20 mesh sieves, formula is: Fructus Crataegi 10 parts, 10 parts of Radix Glycyrrhizae, 10 parts of Fructus Hordei Germinatus, the Radix Astragali 5 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, Rhizoma Atractylodis 5 parts, Rhizoma Dioscoreae 5 parts, Radix Codonopsis 5 parts, Fructus Psoraleae 5 parts, Rhizoma Polygonati 5 parts, Fructus Schisandrae Chinensis 5 parts, 5 parts of Poria, Fructus Lycii 5, Radix Rhodiolae 3 parts, Flos Lonicerae 5 parts, Pericarpium Citri Reticulatae 3 parts;
(2) Chinese herbal medicine substrate preparation: add the Semen Maydis powder of 10% weight ratio and the bean cake powder of 5% weight ratio in the herbal powder after pulverizing, add the water of 2 times, mixing, 121 DEG C of sterilizing 20min;
(3) probiotic's culture: take frozen probiotic bacteria strain respectively, be inoculated in seed culture medium, cultivates 24h, obtains the probiotic bacteria seed liquor of bacterial content >=1 × 108/mL for 35 DEG C;
(4) Chinese herbal medicine probiotics fermention: add pre-incubated bacillus subtilis and Bacillus licheniformis seed liquor in the Chinese herbal medicine substrate after sterilizing by the inoculum concentration of 5%, after 35 DEG C of ferment at constant temperature cultivate 8h, add the seed liquor of bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum again by the inoculum concentration of 2%, 35 DEG C are continued to terminate fermentation after ferment at constant temperature cultivates 24h;
(5) dry: fermentation culture medium adds 3 times of weight Semen Maydis powder, after mixing through vacuum or lyophilization to moisture below 10%, be ground into powder;
(6) batch mixing: add enzyme preparation, mix homogeneously in dried fermentation culture medium, to obtain final product.
Embodiment three
Prepare middle medicinal herbs micro-zoology composite feed additive according to the following steps:
(1) Chinese herbal medicine is pulverized: weighed various Chinese crude drug by formula, mix homogeneously, being crushed to all through 20 mesh sieves, formula is: Fructus Crataegi 10 parts, 10 parts of Radix Glycyrrhizae, 10 parts of Fructus Hordei Germinatus, the Radix Astragali 5 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, Rhizoma Atractylodis 5 parts, Rhizoma Dioscoreae 5 parts, Radix Codonopsis 5 parts, Fructus Psoraleae 5 parts, Rhizoma Polygonati 5 parts, Fructus Schisandrae Chinensis 5 parts, 5 parts of Poria, Fructus Lycii 5, Radix Rhodiolae 3 parts, Flos Lonicerae 5 parts, Pericarpium Citri Reticulatae 3 parts;
(2) Chinese herbal medicine substrate preparation: add the Semen Maydis powder of 10% weight ratio and the bean cake powder of 5% weight ratio in the herbal powder after pulverizing, add the water of 2 times, mixing, 121 DEG C of sterilizing 20min;
(3) probiotic's culture: take frozen probiotic bacteria strain respectively, be inoculated in seed culture medium, cultivates 24h, obtains the probiotic bacteria seed liquor of bacterial content >=1 × 108/mL for 35 DEG C;
(4) Chinese herbal medicine probiotics fermention: add pre-incubated bacillus subtilis and Bacillus licheniformis seed liquor in the Chinese herbal medicine substrate after sterilizing by the inoculum concentration of 5%, after 35 DEG C of ferment at constant temperature cultivate 8h, add the seed liquor of bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum again by the inoculum concentration of 2%, 35 DEG C are continued to terminate fermentation after ferment at constant temperature cultivates 24h;
(5) dry: fermentation culture medium adds 3 times of weight Semen Maydis powder, after mixing through vacuum or lyophilization to moisture below 10%, be ground into powder;
(6) batch mixing: add enzyme preparation, mix homogeneously in dried fermentation culture medium, to obtain final product.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.

Claims (6)

1. a Chinese herbal medicine micro-ecological composite feed additive, including mixture of Chinese herbal medicines, probiotic bacteria, enzyme preparation, it is characterised in that:
Described mixture of Chinese herbal medicines consists of: Fructus Crataegi 10~20 mass parts, Radix Glycyrrhizae 10~20 mass parts, Fructus Hordei Germinatus 10~20 mass parts, the Radix Astragali 5~15 mass parts, the Rhizoma Atractylodis Macrocephalae 5~15 mass parts, Rhizoma Atractylodis 5~10 mass parts, Rhizoma Dioscoreae 0~10 mass parts, Radix Codonopsis 0~10 mass parts, Fructus Psoraleae 0~10 mass parts, Rhizoma Polygonati 0~10 mass parts, Folium Eucommiae 0~10 mass parts, Cortex Magnoliae Officinalis 0~10 mass parts, Radix Puerariae 0~10 mass parts, Semen Coicis 0~10 mass parts, Fructus Schisandrae Chinensis 0~10 mass parts, Poria 0~5 mass parts, Fructus Lycii 0~5 mass parts, Radix Rhodiolae 0~5 mass parts, Flos Lonicerae 0~5 mass parts, Pericarpium Citri tangerinae 0~5 mass parts, flower of poplar 0~5 mass parts, Fructus Aurantii 0~5 mass parts, Pericarpium Citri Reticulatae 0~5 mass parts.
2. a kind of Chinese herbal medicine micro-ecological composite feed additive according to claim 1, it is characterized in that, described probiotic bacteria is bacillus subtilis, Bacillus licheniformis, bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum.
3. a kind of Chinese herbal medicine micro-ecological composite feed additive according to claim 1, it is characterized in that, described enzyme preparation is: the xylanase of the glucoseoxidase of 250U/g, the alpha-galactosidase of 20U/g, the 'beta '-mannase of 500U/g, the phytase of 100U/g and 500U/g.
4. a preparation method for Chinese herbal medicine micro-ecological composite feed additive as claimed in claim 1, it comprises the following steps:
(1) described mixture of Chinese herbal medicines is pulverized: weighed various Chinese crude drug, mix homogeneously by formula, be crushed to all through 20 mesh sieves;
(2) Chinese herbal medicine substrate preparation: add in the mixture of Chinese herbal medicines powder after pulverizing and account for the Semen Maydis powder of 10% weight ratio and the bean cake powder of 5% weight ratio, add the water of 1~3 times, mixing, sterilizing 20min at 121 DEG C;
(3) probiotic's culture: take frozen probiotic bacteria strain respectively, be inoculated in seed culture medium, cultivates 24h at 37 DEG C, obtains the probiotic bacteria seed liquor of bacterial content >=1 × 108/mL;
(4) Chinese herbal medicine probiotics fermention: add pre-incubated bacillus subtilis and Bacillus licheniformis seed liquor in the Chinese herbal medicine substrate after sterilizing by the inoculum concentration of 5%, after 35 DEG C~37 DEG C ferment at constant temperature cultivate 4h~12h, add the seed liquor of bacillus acidophilus, Lactobacillus plantarum, enterococcus faecalis, Candida utilis, saccharomyces cerevisiae and Clostridium butyricum again by the inoculum concentration of 2%, continue to terminate fermentation after ferment at constant temperature cultivates 24h~48h at 30 DEG C~35 DEG C;
(5) dry: in the fermentation culture medium that step (4) obtains, to add the Semen Maydis powder of 1~3 times of weight, after mixing through vacuum or lyophilization to moisture below 10%, be ground into powder;
(6) batch mixing: add enzyme preparation, mix homogeneously in dried fermentation culture medium, to obtain final product.
5. the preparation method of a kind of Chinese herbal medicine micro-ecological composite feed additive according to claim 4, it is characterized in that, described enzyme preparation is: the xylanase of the glucoseoxidase of 250U/g, the alpha-galactosidase of 20U/g, the 'beta '-mannase of 500U/g, the phytase of 100U/g and 500U/g.
6. a using method for the Chinese herbal medicine micro-ecological composite feed additive as described in claim 1 or 4, is addition 0.1~1kg Chinese herbal medicine micro-ecological composite feed additive in animal and fowl fodder per ton, uses after mixing.
CN201610101052.7A 2016-02-24 2016-02-24 Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof Pending CN105724772A (en)

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Application publication date: 20160706