CN105285326A - Preparation method of feed micro-ecologic agent produced by using citrus peels - Google Patents

Preparation method of feed micro-ecologic agent produced by using citrus peels Download PDF

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Publication number
CN105285326A
CN105285326A CN201510688660.8A CN201510688660A CN105285326A CN 105285326 A CN105285326 A CN 105285326A CN 201510688660 A CN201510688660 A CN 201510688660A CN 105285326 A CN105285326 A CN 105285326A
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parts
preparation
cfu
ecologic
orange peel
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黄兴国
彭伟
贺建华
黄其永
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Hunan Agricultural University
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Hunan Agricultural University
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Abstract

The present invention discloses a preparation method of a feed micro-ecologic agent produced by using citrus peels and a product thereof. The preparation method: Lactobacillus delbrueckii (Preservation No., CCTCC M 207096), Bacillus subtilis (Preservation No., CCTCC M 207097), Saccharomyces paradoxus (Preservation No., CCTCC M 207098) are inoculated to the corresponding liquid culture medium and cultured at a constant temperature of 37 DEG C for 18-24 hours, then the prepared bacteria solution is mixed in a certain proportion, the mixture is inoculated into sterilized solid state fermentation medium with the citrus peel as the main raw material and fermented at 30-40 DEG C for 36-48 hours, the fermented mixture is dried at a low temperature of 50-55 DEG C, and the dried mixture is crushed, thereby obtaining the feed micro-ecologic agent. Production technology of the feed micro-ecologic agent has the advantages of low investment, low energy consumption, no waste water pollution, etc., and opens up a new way for the utilization of the citrus peels at the same time.

Description

A kind of preparation method utilizing orange peel to produce feeding micro-ecological preparation
Technical field
The present invention relates to feed industry and livestock breeding industry field, be specifically related to a kind of preparation method utilizing orange peel to produce feeding micro-ecological preparation.
Background technology
Since 20 middle of century find that antibiotic to make an addition to sub-therapeutic dose and have growth-promoting effect in feed, antibiotic has been widely used in husbandry sector, brings certain economic benefit to animal husbandry.At present, no matter be the prevention of livestock and poultry or the treatment of livestock and poultry, still substantially antibiotic is used.But use antibiotic in a large number for a long time, under making pathogenic microorganism produce drug resistance, livestock products drug residue and livestock and poultry body autoimmunity, degradation problem becomes increasingly conspicuous, cause that the generation of livestock and poultry is increased, mortality of livestock and mortality rise year by year, bring huge economic loss to livestock breeding industry.Antibiotic etc also has potential harmfulness to human health by the accumulation of food chain simultaneously.Therefore, measures such as forbidding or limit the use of is taked to antibiotic use in countries in the world, also impels people to find antibiotic substitute as feed addictive simultaneously.
Probiotics is considered to antibiotic effective substitute.Probiotics be use beneficial microbe through fermentation special process of etc.ing make contain viable bacteria and (or) extremely bacterium, comprise the viable bacteria goods of its component and product, have nontoxic, have no side effect, the advantage such as noresidue, and normal flora balance in enteron aisle can be maintained, improve the balance of mucomembranous surface place microorganism or enzyme, improve organism metabolism and strengthen the effects such as immunity of organism.At present, also there is certain problem in commercially available liquid state or solid-state probiotics, and as commercially available liquid probiotics, its production equipment requires that high, energy consumption is large, and the waste water of discharge can contaminated environment; The primary raw material of commercially available solid-state probiotics is the feeding raw material such as dregs of beans, wheat bran, produces probiotics will tie up feedstuff in short supply if be used for, and is difficult to reduce aquaculture cost.Therefore, under the background of built environment friendly, conservation-minded society, develop eco-agriculture and become a kind of trend, particularly under feed resource shortage and the pressure that is growing more intense of industry competition, waste residue is made the feature of environmental protection, low cost feeding micro-ecological preparation significant.
At present, along with the development of Citrus procession industry, there is a large amount of orange peels, only Hunan Province produces orange peel about 200,000 tons per year.Orange peel has nutritive value and medical value, containing nutritional labelings such as abundant carbohydrate, fat, vitamin, amino acid and mineral matters, but the orange peel water content of undried is very high, easily rot, be not suitable for long-distance transport and long-term preservation, that abandons easily causes the waste of resource and the pollution to environment.At present, the orange peel of most domestic is landfilled to abandon and slatterns, only have sub-fraction to be dried and become animal feed as Chinese herbal medicine or ensiling, make animal feed also because of its heavy bitter taste, palatability is poor, protein content is not high, animal absorbs bad and be difficult to industrialization, so imperative to the utilization of orange peel, research, innovation.
Domestic and international for the application of orange peel in herding and research at present: a main approach is exactly that orange peel is processed into feed.The Citrus procession big countries such as annual Brazil, the U.S. are making pellet after the orange peel drying of more than 40%; Add duck feed with the citrus peel meal of 1% ~ 2% according to the study, 2% ~ 3% adds chicken feed, and 5% ~ 10% adds pig feed and adds in ox feed with 10% ~ 20% and feeds, and all obtains good effect.Such as orange peel mixes in pig feed and feeds pigs, more than general pig 50 ~ 100 grams of daily gain.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of and can produce the preparation method of feeding micro-ecological preparation and the feeding micro-ecological preparation of application beneficial microbe many bacterium solid state fermentation orange peel production by efficiency utilization orange peel.This feeding micro-ecological preparation can improve the feed intake of livestock and poultry, improves feed efficiency, growth promoting effects; Immunity moderation function, strengthens resistance against diseases; Substitute feeding antibiotic, reduce diarrhea rate; Reduce nitrogen phosphorus emission in excrement, reduce excreta to the pollution of environment, reducing feed cost and aquaculture cost, is a kind of livestock and poultry high-efficiency and environment-friendly feed additive.
For achieving the above object, the invention provides a kind of preparation method utilizing orange peel to produce feeding micro-ecological preparation, it comprises the following steps:
1) seed liquor is prepared: be the Lactobacillus delbrueckii of CCTCCM207096 by preserving number, preserving number is the Bacillus subtillis of CCTCCM207097, Saccharomyces paradoxus that preserving number is CCTCCM207098 is seeded in MRS fluid nutrient medium, meat soup fluid nutrient medium, potato dextrose broth respectively, cultivate 18 ~ 24 hours at 30 DEG C ~ 37 DEG C;
2) fermentation solid medium is prepared: by weight by air-dry rear orange peel 100 parts, brewex's grains 10 ~ 15 parts, wheat bran 15-20 part, glucose 3 ~ 5 parts, dregs of beans 8 ~ 10 parts, 2 ~ 3 parts, urea, 2 ~ 3 parts, ammonium sulfate, potassium dihydrogen phosphate 0.2 ~ 0.5 part, 0.3 ~ 0.4 part, magnesium sulfate, pulverize respectively, mix after crossing 40 mesh sieves, add distilled water 100 ~ 120 parts, high-temperature sterilization 15-30 minute at fully mixing latter 121 DEG C thoroughly, is cooled to room temperature for subsequent use;
3) solid state fermentation: by step 1) prepare Lactobacillus delbrueckii, Bacillus subtillis, Saccharomyces paradoxus seed liquor 1 ~ 2:1 by volume ~ 2:1 ~ 2 mix, mixed bacteria liquid is joined step 2 in 5-10%V/W ratio) in the solid medium prepared, 30 ~ 40 DEG C of bottom fermentations 36 ~ 48 hours, obtained tunning;
4) tunning is carried out 50 ~ 55 DEG C of low temperature dryings, pulverize, obtain feeding micro-ecological preparation.
The feeding micro-ecological preparation utilizing above-mentioned preparation method to obtain is golden yellow powdery, water content≤10%, and its useful total viable count is respectively: bacillus>=1 × 10 8cFU/g, Bacillus acidi lactici>=1 × 10 8cFU/g, saccharomycete>=2 × 10 8cFU/g.
The beneficial bacterium fermentation of orange peel through filtering out is made feeding micro-ecological preparation by the present invention, be added directly in animal feeding-stuff, the double utility of orange peel and beneficial bacterium can be played, increase beneficial microorganism quantity in animal intestinal, the mineral element that some is necessary is provided, improve the conversion ratio of feed, prevent disease from occurring, and can economic benefit be increased.Microbiological treatment orange peel is utilized to be develop eco-agriculture, the sustainable development path of reasonable employment waste resource, available protecting environment.Yet there are no the report that many bacterium solid state fermentation orange peel produces feeding micro-ecological preparation aspect up to now.
The invention has the advantages that:
1, the present invention has hewed out the scientific and reasonable regeneration approach of orange peel, thus solve its pollution problem to environment, there is provided possibility for Citrus procession industry realizes zero-emission, not only also can create economic benefit for enterprise has saved pollution treatment expense, meet the strategy of sustainable development.
2, the present invention adopts many bacterium solid-state fermentation process to produce feeding micro-ecological preparation, and easy to process, energy consumption is low, produces and throws than high, and whole the process not only easy to control but also sorrow of no waste discharge, can realize cleaner production.
3, the raw material of the present invention more than 75% is orange peel and other slag class materials, and its abundance, cheap, production cost is low, can improve the competitiveness of enterprise.
Detailed description of the invention
Embodiment 1:
1) Lactobacillus delbrueckii (preserving number CCTCCM207096), Bacillus subtillis (preserving number CCTCCM207097), Saccharomyces paradoxus (preserving number CCTCCM207098) are seeded in 100mlMRS fluid nutrient medium, 100ml meat soup fluid nutrient medium, 100ml potato dextrose broth respectively, under 37 DEG C of constant temperature, cultivate 24 hours obtained seed liquor;
2) by weight by air-dry rear orange peel 100 parts, brewex's grains 10 parts, 15 parts, wheat bran, glucose 3 parts, dregs of beans 8 parts, 2 parts, urea, 2 parts, ammonium sulfate, potassium dihydrogen phosphate 0.2 part, 0.3 part, magnesium sulfate, pulverize respectively, mix after crossing 40 mesh sieves, add distilled water 100 parts, high-temperature sterilization 15 minutes at fully mixing latter 121 DEG C thoroughly, be cooled to room temperature for subsequent use, obtain solid medium;
3) by the 1:1:1 mixing by volume of three of above-mentioned preparation kinds of seed liquor, gained mixed bacteria liquid being joined step 2 in 10% (V/W) ratio) in the solid medium of gained, 32 DEG C of bottom fermentations 36 hours, obtain tunning;
4) tunning is carried out 50 DEG C of low temperature dryings, pulverize, obtain feeding micro-ecological preparation.
The probiotics that this technique obtains is golden yellow powdery, water content≤10%, and its useful total viable count is respectively: Lactobacillus delbrueckii 1 × 10 8cFU/g ~ 1 × 10 9cFU/g, Bacillus subtillis 1 × 10 8cFU/g ~ 4 × 10 9cFU/g, Saccharomyces paradoxus 2 × 10 8cFU/g ~ 1 × 10 9cFU/g.
Embodiment 2:
1) Lactobacillus delbrueckii (preserving number CCTCCM207096), Bacillus subtillis (preserving number CCTCCM207097), Saccharomyces paradoxus (preserving number CCTCCM207098) are seeded in 100mlMRS fluid nutrient medium, 100ml meat soup fluid nutrient medium, 100ml potato dextrose broth respectively, under 30 DEG C of constant temperature, cultivate 21 hours obtained seed liquor;
2) by weight by air-dry rear orange peel 100 parts, brewex's grains 12 parts, 17 parts, wheat bran, glucose 4 parts, dregs of beans 9 parts, 2.5 parts, urea, 2.5 parts, ammonium sulfate, potassium dihydrogen phosphate 0.35 part, 0.35 part, magnesium sulfate, pulverize respectively, mix after crossing 40 mesh sieves, add distilled water 110 parts, high-temperature sterilization 30 minutes at fully mixing latter 121 DEG C thoroughly, be cooled to room temperature for subsequent use, obtain solid medium;
3) by the 2:2:2 mixing by volume of three of above-mentioned preparation kinds of seed liquor, mixed bacteria liquid is joined in sterilized solid medium in 7% (V/W) ratio, 35 DEG C of bottom fermentations 42 hours, obtain tunning;
4) tunning is carried out 52 DEG C of low temperature dryings, pulverize, obtain feeding micro-ecological preparation.
The probiotics that this technique obtains is golden yellow powdery, water content≤10%, and its useful total viable count is respectively: Bacillus acidi lactici 1 × 10 8cFU/g ~ 1 × 10 9cFU/g, bacillus 1 × 10 8cFU/g ~ 4 × 10 9cFU/g, saccharomycete 2 × 10 8cFU/g ~ 1 × 10 9cFU/g.
Embodiment 3:
1) Lactobacillus delbrueckii (preserving number CCTCCM207096), Bacillus subtillis (preserving number CCTCCM207097), Saccharomyces paradoxus (preserving number CCTCCM207098) are seeded in 100mlMRS fluid nutrient medium, 100ml meat soup fluid nutrient medium, 100ml potato dextrose broth respectively, under 34 DEG C of constant temperature, cultivate 18 hours obtained seed liquor;
2) by weight by air-dry rear orange peel 100 parts, brewex's grains 15 parts, 20 parts, wheat bran, glucose 5 parts, dregs of beans 10 parts, 3 parts, urea, 3 parts, ammonium sulfate, potassium dihydrogen phosphate 0.5 part, 0.4 part, magnesium sulfate, pulverize respectively, mix after crossing 40 mesh sieves, add distilled water 120 parts, high-temperature sterilization 20 minutes at fully mixing latter 121 DEG C thoroughly, for subsequent use to room temperature after cooling, obtain solid medium;
3) by the 2:1:2 mixing by volume of three of above-mentioned preparation kinds of seed liquor, mixed bacteria liquid is joined in sterilized solid medium in 5% (V/W) ratio, 40 DEG C of bottom fermentations 48 hours, obtain tunning;
4) tunning is carried out 55 DEG C of low temperature dryings, pulverize, obtain feeding micro-ecological preparation.
The probiotics that this technique obtains is golden yellow powdery, water content≤10%, and its useful total viable count is respectively: Bacillus acidi lactici 1 × 10 8cFU/g ~ 1 × 10 9cFU/g, bacillus 1 × 10 8cFU/g ~ 4 × 10 9cFU/g, saccharomycete 2 × 10 8cFU/g ~ 1 × 10 9cFU/g.
The probiotics prepared below by test explanation the present invention is on the impact of growth of animals or poultry:
Test 1:
Select that health, age in days are close, the grower pigs of original body mass about 28kg (Du × long × large) 48, be divided into 3 process at random, often organize 4 repetitions, often repetition 4 pigs, male and female half and half.Control group is only fed basal diet, and antibiotic group is fed basal diet+75mg/kg aureomycin, and test group is fed the probiotics of basal diet+2000mg/kg.Raise 7 days phases in advance, 28 days experimental periods.
Test data following (table 1-4):
Table 1 probiotics is on the impact of grower pigs growth performance
Table 2 probiotics is on the impact of grower pigs nutrient digestibility
Table 3 probiotics is on the impact of grower pigs physiochemical indice
Table 4 probiotics is on the impact of grower pigs N, P discharge capacity
From result of the test, add orange peel and can improve grower pigs daily ingestion amount through the probiotics made that ferments, improve daily gain and reduce feedstuff-meat ratio, the apparent digestibility of the nutriment such as crude protein, fat and capacity usage ratio improve, and substitute antibiotics is applied in feed.In addition, also can improve body immunity and reduce N, P discharge capacity.
Test 2:
The blue or green pin fiber crops chicken that health, age in days are close, original body mass is close is selected in this test, selects 120 1 Day-old Broiler Chickens, is divided into 4 groups at random, often organize three repetitions, each repetition 10 chickens.Adopt bedding and padding flat foster.Blank group is fed basal diet, control group fed basal diet+antibiotic, and test group 1 is fed basal diet+0.1% probiotics, test group 2 basal diet+0.2% probiotics.Experimental period is 28 days.
Test data following (table 5):
Table 5 probiotics is on the impact of chicken production performance
From result of the test, add the ferment probiotics made of orange peel and can improve chick and improve daily gain and reduction feedstuff-meat ratio, the application of substitute antibiotics in feed.

Claims (2)

1. utilize orange peel to produce a preparation method for feeding micro-ecological preparation, it is characterized in that comprising the following steps:
1) seed liquor is prepared: be the Lactobacillus delbrueckii of CCTCCM207096 by preserving number, preserving number is the Bacillus subtillis of CCTCCM207097, Saccharomyces paradoxus that preserving number is CCTCCM207098 is seeded in MRS fluid nutrient medium, meat soup fluid nutrient medium, potato dextrose broth respectively, cultivate 18 ~ 24 hours at 30 DEG C ~ 37 DEG C;
2) fermentation solid medium is prepared: by weight by air-dry rear orange peel 100 parts, brewex's grains 10 ~ 15 parts, wheat bran 15-20 part, glucose 3 ~ 5 parts, dregs of beans 8 ~ 10 parts, 2 ~ 3 parts, urea, 2 ~ 3 parts, ammonium sulfate, potassium dihydrogen phosphate 0.2 ~ 0.5 part, 0.3 ~ 0.4 part, magnesium sulfate, pulverize respectively, mix after crossing 40 mesh sieves, add distilled water 100 ~ 120 parts, high-temperature sterilization 15-30 minute at fully mixing latter 121 DEG C thoroughly, is cooled to room temperature for subsequent use;
3) solid state fermentation: by step 1) prepare Lactobacillus delbrueckii, Bacillus subtillis, Saccharomyces paradoxus seed liquor 1 ~ 2:1 by volume ~ 2:1 ~ 2 mix, mixed bacteria liquid is joined step 2 in 5-10%V/W ratio) in the solid medium prepared, 30 ~ 40 DEG C of bottom fermentations 36 ~ 48 hours, obtained tunning;
4) tunning is carried out 50 ~ 55 DEG C of low temperature dryings, pulverize, obtain feeding micro-ecological preparation.
2. the feeding micro-ecological preparation utilizing preparation method described in claim 1 to obtain, is characterized in that: this feeding micro-ecological preparation is golden yellow powdery, water content≤10%, and its useful total viable count is respectively: bacillus>=1 × 10 8cFU/g, Bacillus acidi lactici>=1 × 10 8cFU/g, saccharomycete>=2 × 10 8cFU/g.
CN201510688660.8A 2015-10-22 2015-10-22 Preparation method of feed micro-ecologic agent produced by using citrus peels Pending CN105285326A (en)

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CN107094993A (en) * 2017-05-11 2017-08-29 李平作 A kind of manufacture method of feature glossy ganoderma fermentation feed
CN107467384A (en) * 2017-08-04 2017-12-15 池州市兴牧饲料有限公司 A kind of high efficiency composition feed addictive
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CN106509340A (en) * 2016-11-17 2017-03-22 平坝县鸿黔养殖场 Producing method of livestock feed
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CN107094993A (en) * 2017-05-11 2017-08-29 李平作 A kind of manufacture method of feature glossy ganoderma fermentation feed
CN107467384A (en) * 2017-08-04 2017-12-15 池州市兴牧饲料有限公司 A kind of high efficiency composition feed addictive
CN108477403A (en) * 2017-11-21 2018-09-04 湖南帝亿生物科技股份有限公司 The preparation method of one boar high-effective microorganism fermented feed

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Application publication date: 20160203