CN103070291A - Wet feed and preparing method thereof - Google Patents

Wet feed and preparing method thereof Download PDF

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CN103070291A
CN103070291A CN201310043869XA CN201310043869A CN103070291A CN 103070291 A CN103070291 A CN 103070291A CN 201310043869X A CN201310043869X A CN 201310043869XA CN 201310043869 A CN201310043869 A CN 201310043869A CN 103070291 A CN103070291 A CN 103070291A
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fermentation
preparation
citrus peel
peel residue
accounts
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CN103070291B (en
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彭荣
郑旭煦
殷钟意
雷星
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Chongqing Technology and Business University
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Abstract

The invention provides a preparing method of a wet feed. The method comprises the following steps of inoculating aerobic fermenting bacteria into a fermenting substrate to carry out aerobic fermenting, wherein the fermenting substrate comprises citrus pomace, and the aerobic fermenting bacteria comprise endomycopsis fibuligera, aspergillus niger and aspergillus oryzae; and inoculating an anaerobic fermenting bacteria liquid into the aerobically-fermented material, and carrying out anaerobic fermenting to obtain the wet feed. The method has the advantages that the citrus pomace is used as the raw material and is sequentially subjected to the aerobic fermenting and the anaerobic fermenting, and then the wet feed can be prepared without crushing, drying and the like; the process is simple, and the energy consumption is lower; the prepared wet feed can be directly used for feeding and can also be stored for a long time, and the storage life of the fermented feed is prolonged; and the palatability is good, so the wet feed is suitable for feeding animals of multiple types, and is especially suitable for ruminant animals, such as cows and sheep.

Description

Moist diet and preparation method thereof
Technical field
The invention belongs to the technique for producing feed field, relate in particular to a kind of moist diet and preparation method thereof.
Background technology
Oranges and tangerines are one of south China main product fruit, and output is only second to the U.S. and Brazil, are mainly used in squeezing the juice.But, the skin slag of meeting residual about 60% after oranges and tangerines are squeezed the juice, a large amount of skin slags not only need large quantity space to stack, the waste resource, and also the skin slag can rot in the stacking process, contaminated environment.
In order to improve the comprehensive utilization value of oranges and tangerines, improve the profit of oranges and tangerines processing enterprise, reduce the wasting of resources and environmental pollution, prior art is generally with citrus peel residue and high nutrient, such as mixing such as corn flour, inoculation yeast, the wood microorganism such as mould stir fermentation and are prepared into fermented feed in round.As, (the red tail feathers of Lee such as the red tail feather of Lee, Li Yan, Yu builds, the research of citrus peel residue fermented feed, food industry science and technology, 05 phase in 2009) at first citrus peel residue is dried to constant weight under 105 ℃, be mixed with inoculation yeast bacterium behind the solid medium, by fermentation after, the tunning that obtains is obtained fermented feed through processing such as squeezing, drying, pulverizing.But this method needs the fermentate that water content is high to carry out the drying processing after fermentation is complete, and not only technique is loaded down with trivial details, power consumption is huge, and can cause the loss of tunning nutritional labeling.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is to provide a kind of preparation method of moist diet, and preparation method's technique provided by the invention is simple, power consumption is less, and the moist diet storage life for preparing is longer.
The invention provides a kind of preparation method of moist diet, may further comprise the steps:
Inoculate the laggard aerobe fermentation of acting charitably of aerobic fermentation bacterium in fermentation substrate, described fermentation substrate comprises citrus peel residue, and described aerobic fermentation bacterium comprises Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae;
Inoculation anaerobic fermentation bacterium liquid carries out obtaining moist diet behind the anaerobic fermentation in the material behind the described aerobic fermentation.
Preferably, described Endomycopsis Fibnligera accounts for the 0.55wt% ~ 0.95wt% of described citrus peel residue; Described aspergillus niger accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue; Described aspergillus oryzae accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue.
Preferably, described fermentation substrate also comprises the straw that accounts for described citrus peel residue 5wt% ~ 10wt%.
Preferably, the length of described straw is 4cm ~ 6cm.
Preferably, described fermentation substrate also comprises the wheat bran that accounts for described citrus peel residue 1wt% ~ 2wt%; With the urea that accounts for described citrus peel residue 0.8wt% ~ 2wt%.
Preferably, the time of described aerobic fermentation is 48h ~ 72h, and temperature is 20 ℃ ~ 40 ℃.
Preferably, described anaerobic fermentation bacterium liquid comprises Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water.
Preferably, the mass ratio of described Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water is 30 ~ 50:30 ~ 50:10 ~ 30:10000.
Preferably, the ratio of the quality of the volume of described anaerobic fermentation bacterium liquid and the material behind the described aerobic fermentation is 6L ~ 7L:1 ton.
The present invention also provides a kind of moist diet for preparing according to the described method of technique scheme.
Compared with prior art, the present invention is take citrus peel residue as fermentation substrate, at first inoculation comprises that the aerobic fermentation bacterium of Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae carries out aerobic fermentation, then inoculate anaerobic fermentation bacterium liquid and carry out anaerobic fermentation, simultaneously, anaerobic fermentation process also is the storage process of feed, and namely the product in the anaerobic fermentation process can with getting with usefulness, all can satisfy the requirement of ensilage.The present invention is take citrus peel residue as major ingredient, pass through successively aerobic fermentation and anaerobic fermentation, need not the processing such as squeezing, drying and can prepare moist diet, not only technique is simple, and energy consumption is lower, and the moist diet for preparing can use by Direct-fed, also can long preservation, prolonged the shelf-life of fermented feed, palatability is good, be fit to many animals and feed, the feed that especially is suitable as the ruminant animals such as cattle and sheep uses.Experimental result shows, the main nutrient composition of the feed that the present invention prepares can reach the standard of industry standard SB/T10261-1996 cow concentrate supplement, preserves that the main nutrient composition of feed also can satisfy above-mentioned standard after 4 months.
The specific embodiment
The invention provides a kind of preparation method of moist diet, may further comprise the steps:
Inoculate the laggard aerobe fermentation of acting charitably of aerobic fermentation bacterium in fermentation substrate, described fermentation substrate comprises citrus peel residue, and described aerobic fermentation bacterium comprises Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae;
Inoculation anaerobic fermentation bacterium liquid carries out obtaining moist diet behind the anaerobic fermentation in the material behind the described aerobic fermentation.
The present invention prepares moist diet take citrus peel residue as fermentation substrate, and described citrus peel residue refers to the residue skin slag after oranges and tangerines are squeezed the juice.The present invention preferably adopts fresh citrus peel residue, i.e. the citrus peel residue of undried processing.
Described fermentation substrate preferably also comprises straw, and described straw preferably accounts for the 5wt% ~ 10wt% of described citrus peel residue, more preferably accounts for 6wt% ~ 8wt%.Described straw is preferably dry straw joint, and the length of described straw joint is preferably 4cm ~ 6cm.Those skilled in the art can carry out according to the water content of citrus peel residue the adjustment of straw addition, and the present invention there is no particular restriction to this.
Described fermentation substrate preferably also comprises wheat bran and urea, and described wheat bran preferably accounts for the 1wt% ~ 2wt% of described citrus peel residue, more preferably accounts for 1.2wt% ~ 1.8wt%; Described urea preferably accounts for the 0.8wt% ~ 2wt% of described citrus peel residue, more preferably accounts for 1wt% ~ 1.8wt%.
The present invention inoculation aerobic fermentation bacterium in the described fermentation substrate carries out aerobic fermentation, and described aerobic fermentation bacterium comprises Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae.Wherein, described Endomycopsis Fibnligera preferably accounts for the 0.55wt% ~ 0.95wt% of described citrus peel residue, more preferably 0.6wt% ~ 0.9wt%; Described aspergillus niger preferably accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue, more preferably 0.3wt% ~ 0.6wt%; Described aspergillus oryzae preferably accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue, more preferably 0.3wt% ~ 0.6wt%.
In the present invention, described aerobic fermentation bacterium is preferably the Mixed Microbes of cultivating through enlarging, and preferably obtains in accordance with the following methods:
Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae according to method process slant tube kind well known to those skilled in the art, are turned the triangular flask kind and turn thin layer fermentation koji tray kind again, obtain comprising the Mixed Microbes of Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae.
The present invention enlarges the mode of cultivating to above-mentioned three grades and there is no particular restriction, cultivates according to condition well known to those skilled in the art to get final product.
Particularly, the present invention is with citrus peel residue, straw, wheat bran, urea with comprise that the Mixed Microbes of Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae mixes, and carries out aerobic fermentation.Preferably, above-mentioned aerobic fermentation carries out in the cement fermentation vat, preferably sets up bamboo clappers in cement fermentation vat bottom, and bamboo clappers is arranged with ventilation blower and ajutage, described mixed material is piled up in the enterprising aerobe fermentation of acting charitably of bamboo clappers, and described mixed material thickness is preferably 20cm ~ 25cm.In the present invention, the temperature of described aerobic fermentation is preferably 0 ℃ ~ 50 ℃, and more preferably 20 ℃ ~ 40 ℃, most preferably be 30 ℃ ~ 40 ℃, the time is preferably 48h ~ 72h, more preferably 50h ~ 70h.The present invention preferably with mixed material at natural temperature bottom fermentation 6h ~ 8h, then mixed material is remained under 20 ℃ ~ 40 ℃ and carries out aerobic fermentation.
After aerobic fermentation is complete, in the material that obtains, inoculate anaerobic fermentation bacterium liquid, carry out anaerobic fermentation.In the present invention, described anaerobic fermentation bacterium liquid preferably includes Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water, the mass ratio of described Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water is preferably 30 ~ 50:30 ~ 50:10 ~ 30:10000, more preferably 35 ~ 45:35 ~ 45:15 ~ 25:10000.The present invention does not have particular restriction to the preparation method of described anaerobic fermentation bacterium liquid, can prepare in accordance with the following methods:
Lactobacillus plantarum, streptococcus fecalis and saccharomycete dry bacteria and water are mixed, and room temperature is placed 5h ~ 6h and be get final product.
In the present invention, the ratio of the quality of the volume of described anaerobic fermentation bacterium liquid and the material behind the described aerobic fermentation is preferably 6L ~ 7L:1 ton, more preferably 6.2L ~ 6.7L.Material is at the effect bottom fermentation of anaerobic fermentation bacterium, and the material after the fermentation gets final product ready access upon use.
Described anaerobic fermentation preferably carries out in cement after fermentation cellar for storing things, and the volume at described cement after fermentation cellar for storing things can be built according to fermentation materials, and large-scale cellar for storing things preferably arranges the charging gradient, makes things convenient for the automobile turnover.Particularly, after aerobic fermentation finishes, the material that obtains is transferred in the cement after fermentation cellar for storing things, spray anaerobic fermentation bacterium liquid after preferably piling up the thick material of 20cm ~ 30cm, successively compress material, carry out anaerobic fermentation after the sealing, but the material ready access upon use in the anaerobic fermentation process.Preferably, the present invention preferably sprays salt at the superiors' material before sealing.
The present invention also provides a kind of moist diet for preparing according to the described method of technique scheme, and the water content of described moist diet is preferably 70wt% ~ 83wt%, more preferably 72wt% ~ 82wt%; The crude protein content of described moist diet is preferably 15wt% ~ 20wt%, more preferably 17wt%19wt%; The crude fiber content of described moist diet is preferably 11wt% ~ 16wt%, more preferably 13wt% ~ 15wt%; The soluble polysaccharide content of described moist diet is preferably 2wt% ~ 5wt%, more preferably 2.5wt% ~ 3.5wt%; The pH value of described moist diet is preferably 3 ~ 4.5, and more preferably 3.4 ~ 4.In the present invention, the content of crude protein content, crude fiber content and soluble polysaccharide is respectively the over dry content of material.
In the process of the above-mentioned moist diet of preparation, respectively after anaerobic fermentation carries out 1 month, 2 months, 3 months and 4 months, sampling detects analysis, the result shows that the main nutrient composition of the moist diet that obtains can reach the standard of industry standard SB/T10261-1996 cow concentrate supplement, and the ensiling quality also can satisfy the requirement of ensilage.The result shows, the time after the slagging of squeezing the juice after oranges and tangerines are gathered calculates, and method provided by the invention can make citrus peel residue from then May that is saved in Second Year December.
The moist diet that the present invention prepares can Direct-fed uses, also can long preservation, prolonged the shelf-life of fermented feed, and palatability is good, is fit to many animals and feeds, and the feed that especially is suitable as the ruminant animals such as cattle and sheep uses.In addition, method provided by the invention is lower to equipment requirement, both has been applicable to peasant household's self-control personal, and popularization also can be mass-produced.
The present invention is take citrus peel residue as fermentation substrate, at first inoculation comprises that the aerobic fermentation bacterium of Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae carries out aerobic fermentation, then inoculate anaerobic fermentation bacterium liquid and carry out anaerobic fermentation, simultaneously, anaerobic fermentation process also is the storage process of feed, be that product in the anaerobic fermentation process can with getting with usefulness, all can satisfy the requirement of ensilage.The present invention passes through aerobic fermentation and anaerobic fermentation successively take citrus peel residue as major ingredient, need not the processing such as squeezing, drying and can prepare moist diet, not only technique is simple, and energy consumption is lower, and cost of material is cheap, take full advantage of resource, can not cause environmental pollution.
In order to further specify the present invention, below in conjunction with embodiment moist diet provided by the invention and preparation method thereof is described in detail.
Embodiment 1 ~ 5
According to the part by weight shown in the table 1 Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae are passed through the slant tube kind respectively, turn the triangular flask kind and turn again thin layer fermentation koji tray kind, obtain comprising the Mixed Microbes of Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae;
Fresh citrus peel residue, wheat bran, urea and straw after will squeezing the juice according to the part by weight shown in the table 1 respectively, inoculate respectively above-mentioned Mixed Microbes after, room temperature bottom fermentation 5 days ~ 6 days obtains the material behind the aerobic fermentation; To the material behind the described aerobic fermentation and the fresh citrus peel residue after squeezing the juice detect, the result is referring to table 2, the performance indications of material behind the aerobic fermentation that table 2 provides for the embodiment of the invention 1 ~ 5;
Be Lactobacillus plantarum, streptococcus fecalis and the saccharomycete dry bacteria of 40:40:20 and water according to weight ratio with mass ratio be that the ratio of 1:100 mixes, place under the room temperature and obtain anaerobic fermentation bacterium liquid after 5 ~ 6 hours;
The material spray that obtains behind the described aerobic fermentation is respectively spilt and is stated anaerobic fermentation bacterium liquid, material per ton carries out anaerobic fermentation after spraying 6 ~ 7L anaerobic fermentation bacterium liquid, behind the anaerobic fermentation 2 months, the moist diet that obtains is detected, the result is referring to table 3, the performance indications of the moist material that table 3 provides for the embodiment of the invention 1 ~ 5;
Respectively embodiment 1 anaerobic fermentation 1 month, 2 months, the moist diet that obtained in 3 months and 4 months are detected, the result is referring to table 4, the retention index of the moist material that table 4 provides for the embodiment of the invention 1.
The raw material proportioning that table 1 embodiment of the invention 1 ~ 5 provides
Figure BDA00002816426800061
In the table 1, the addition of Endomycopsis Fibnligera, aspergillus oryzae and aspergillus niger is the mass percent that accounts for citrus peel residue.
The performance indications of material behind the aerobic fermentation that table 2 embodiment of the invention 1 ~ 5 provides
Figure BDA00002816426800062
In the table 2, crude protein content and crude fiber content are over dry material percentage composition.
The performance indications of the moist material that table 3 provides for the embodiment of the invention 1 ~ 5
Figure BDA00002816426800063
In the table 3, crude protein content, crude fiber content and soluble polysaccharide content are over dry material percentage composition.
The retention index of the moist material that table 4 embodiment of the invention 1 provides
Figure BDA00002816426800071
In the table 4, crude protein content, crude fiber content and soluble polysaccharide content are over dry material percentage composition.
By above-described embodiment as can be known, the main nutrient composition of the moist diet that the present invention prepares can reach the standard of industry standard SB/T10261-1996 cow concentrate supplement, and the ensiling quality also can satisfy the requirement of ensilage; But the moist diet long preservation of the present invention's preparation still can satisfy above-mentioned standard between storage life.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (10)

1. the preparation method of a moist diet may further comprise the steps:
Inoculate the laggard aerobe fermentation of acting charitably of aerobic fermentation bacterium in fermentation substrate, described fermentation substrate comprises citrus peel residue, and described aerobic fermentation bacterium comprises Endomycopsis Fibnligera, aspergillus niger and aspergillus oryzae;
Inoculation anaerobic fermentation bacterium liquid carries out obtaining moist diet behind the anaerobic fermentation in the material behind the described aerobic fermentation.
2. preparation method according to claim 1 is characterized in that, described Endomycopsis Fibnligera accounts for the 0.55wt% ~ 0.95wt% of described citrus peel residue; Described aspergillus niger accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue; Described aspergillus oryzae accounts for the 0.25wt% ~ 0.65wt% of described citrus peel residue.
3. preparation method according to claim 1 is characterized in that, described fermentation substrate also comprises the straw that accounts for described citrus peel residue 5wt% ~ 10wt%.
4. preparation method according to claim 3 is characterized in that, the length of described straw is 4cm ~ 6cm.
5. according to claim 3 or 4 described preparation methods, it is characterized in that, described fermentation substrate also comprises the wheat bran that accounts for described citrus peel residue 1wt% ~ 2wt%; With the urea that accounts for described citrus peel residue 0.8wt% ~ 2wt%.
6. preparation method according to claim 1 is characterized in that, the time of described aerobic fermentation is 48h ~ 72h, and temperature is 20 ℃ ~ 40 ℃.
7. preparation method according to claim 1 is characterized in that, described anaerobic fermentation bacterium liquid comprises Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water.
8. preparation method according to claim 7 is characterized in that, the mass ratio of described Lactobacillus plantarum, streptococcus fecalis, saccharomycete and water is 30 ~ 50:30 ~ 50:10 ~ 30:10000.
9. preparation method according to claim 6 is characterized in that, the ratio of the volume of described anaerobic fermentation bacterium liquid and the quality of the material behind the described aerobic fermentation is 6L ~ 7L:1 ton.
10. the moist diet for preparing according to the described method of claim 1 ~ 9 any one.
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CN105053656A (en) * 2015-08-10 2015-11-18 桂林鲜牧饲料发展有限公司 Wet biologic feed without antibiotics or hormones for flocks and herds and preparation method of wet biologic feed
CN105053537A (en) * 2015-08-10 2015-11-18 中国科学院重庆绿色智能技术研究院 High protein feed producing method using orange peel residues as raw materials and feed
CN105053593A (en) * 2015-07-15 2015-11-18 北京生泰尔科技股份有限公司 Traditional Chinese medicine residues prepared poultry feed additive and preparation method thereof
CN105285360A (en) * 2015-10-27 2016-02-03 重庆工商大学 TMR fermentation ration containing citrus pulp for beef cattle and preparation method of TMR fermentation ration
CN105285326A (en) * 2015-10-22 2016-02-03 湖南农业大学 Preparation method of feed micro-ecologic agent produced by using citrus peels
CN105746903A (en) * 2016-03-10 2016-07-13 安徽省全椒未来饲料有限责任公司 Production and fermentation process of plant protein feed
CN107259103A (en) * 2017-07-20 2017-10-20 邵阳心连心食品有限公司 Orange peel residue fermented feed production technology
CN107279464A (en) * 2017-08-10 2017-10-24 成都钧乔农业科技开发有限公司 A kind of technique for being engineered fermentation of biomass feed
CN107712311A (en) * 2017-11-28 2018-02-23 佛山宝来迪生物科技有限公司 A kind of manufacture method and feed of pineapple peel probiotic feed
CN107744052A (en) * 2017-11-28 2018-03-02 佛山宝来迪生物科技有限公司 A kind of reuse method of pineapple peel
CN107889954A (en) * 2017-11-28 2018-04-10 佛山宝来迪生物科技有限公司 A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method
CN108065113A (en) * 2016-11-08 2018-05-25 佛山市珠水生物科技有限公司 A kind of aquatic products fermented feed and preparation method thereof, application
CN112205517A (en) * 2020-09-30 2021-01-12 广西壮族自治区农业科学院 Chicken feed for improving laying rate and preparation method thereof

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CN105053593A (en) * 2015-07-15 2015-11-18 北京生泰尔科技股份有限公司 Traditional Chinese medicine residues prepared poultry feed additive and preparation method thereof
CN105053537A (en) * 2015-08-10 2015-11-18 中国科学院重庆绿色智能技术研究院 High protein feed producing method using orange peel residues as raw materials and feed
CN105053656A (en) * 2015-08-10 2015-11-18 桂林鲜牧饲料发展有限公司 Wet biologic feed without antibiotics or hormones for flocks and herds and preparation method of wet biologic feed
CN105053537B (en) * 2015-08-10 2019-01-01 中国科学院重庆绿色智能技术研究院 It is a kind of using orange peel slag as the production method of the high protein feed of raw material and feed
CN105285326A (en) * 2015-10-22 2016-02-03 湖南农业大学 Preparation method of feed micro-ecologic agent produced by using citrus peels
CN105285360A (en) * 2015-10-27 2016-02-03 重庆工商大学 TMR fermentation ration containing citrus pulp for beef cattle and preparation method of TMR fermentation ration
CN105746903A (en) * 2016-03-10 2016-07-13 安徽省全椒未来饲料有限责任公司 Production and fermentation process of plant protein feed
CN108065113A (en) * 2016-11-08 2018-05-25 佛山市珠水生物科技有限公司 A kind of aquatic products fermented feed and preparation method thereof, application
CN107259103A (en) * 2017-07-20 2017-10-20 邵阳心连心食品有限公司 Orange peel residue fermented feed production technology
CN107279464A (en) * 2017-08-10 2017-10-24 成都钧乔农业科技开发有限公司 A kind of technique for being engineered fermentation of biomass feed
CN107279464B (en) * 2017-08-10 2021-03-30 成都钧乔农业科技开发有限公司 Process for engineering fermentation of biomass feed
CN107744052A (en) * 2017-11-28 2018-03-02 佛山宝来迪生物科技有限公司 A kind of reuse method of pineapple peel
CN107889954A (en) * 2017-11-28 2018-04-10 佛山宝来迪生物科技有限公司 A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method
CN107712311A (en) * 2017-11-28 2018-02-23 佛山宝来迪生物科技有限公司 A kind of manufacture method and feed of pineapple peel probiotic feed
CN112205517A (en) * 2020-09-30 2021-01-12 广西壮族自治区农业科学院 Chicken feed for improving laying rate and preparation method thereof

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