CN102283314A - Method for preparing feed by using microorganism to treat purple arrowroot dregs - Google Patents
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Abstract
The invention discloses a method for preparing feed by using a microorganism to treat purple arrowroot dregs, which relates to feed. The microorganism consists of five kinds of microbial additives for feeding, which are approved by the agriculture ministry to use, namely bacillus subtilis, lactobacillus lactis, lactobacillus casei, candida utilis and saccharomyces cerevisiae. A microbial preparation is prepared; then the arrowroot dregs are crushed; the microbial preparation is added in the crushed arrowroot dregs; and the mixture is sealed and fermented to obtain a feed product. The animal feed is prepared by using the compound microbial preparation to treat the purple arrowroot dregs; the purple arrowroot dregs are fermented; a new source for fermenting the feed is opened up; and meanwhile, resources are reasonably and comprehensively utilized and wastes are changed into valuables. The development and utilization levels of feed resources can be improved, the supply of feed raw materials is increased, grain consumption is reduced and the shortage of the feed resources can be relieved to a certain degree.
Description
Technical field
The present invention relates to a kind of feed, especially relate to the method that a kind of microbiological treatment edible canna slag prepares feed.
Background technology
China is first populous nation in the world, supports the population in the world 22% with the soil in the world 7%, and food supply is very nervous.Along with the fast development of China's aquaculture, the feed resource shortage problem of herding main producing region is also more and more serious, and energy feed is under-supply, and protein feeds is seriously deficient, the degree of self-sufficiency is low, even the situation that people and animals strive grain occurs.Part is cultured and Feed Enterprise is blindly carried out American-European corn bean-type daily ration pattern, causes the anxiety of high-quality feed resources such as China's dregs of beans, fish meal and corn.At the present situation of China's feed resource critical shortage, improve the development of exploitation level of China's routine and unconventional feed resource, the supply that increases feedstuff is a main working tasks from now on to alleviate China's feed resource shortage.On the other hand, in recent years, the drawback that antibiotic abuse brought is familiar with by people day by day, and in animal husbandry, the use of the probiotics of nontoxic, harmless, noresidue more and more comes into one's own.
Probiotics has another name called probiotic, bacteria-promoting agent, live bacteria agent.Animal microecological formulation is a kind of of veterinary biological product, it is to be used to regulate the animal body microecological balance according to animal microecology basic principle development, strengthen animal and come prevent disease to the inhibitory action of intestinal toxic microorganism or by strengthening non-specific immune function, thereby promotion growth of animal or a class medicine of improving food conversion ratio or feed addictive (Lei Zhihu. the effect of probiotics and application, Shanxi agricultural animal and veterinary, 2008.2:16-17).In the feed addictive kind catalogue that the permission that No. 105 bulletin of China Ministry of Agriculture announced is used, feed level microbe additive is 12 kinds, is respectively: Lactobacillus casei, Lactobacillus plantarum, lactobacillus acidophilus, streptococcus fecalis, urine streptococcus, Pediococcus acidilactici, bacillus subtilis, bafillus natto, streptococcus lactis, brewer's yeast, candida utili, Rhodopseudomonas palustris.Fermentative feedstuff of microbe is to utilize breeding thalline of microorganism and the feed that metabolism is produced and modulated, it mainly is to utilize the fermentation of microorganism to change the physical and chemical character of feed, reach the effect that increases palatability and nutritive value, and improve digestive utilization ratio.The used bacterial classification major part of fermented feed is a probiotics.Ole etc. raise object, investigated the influence of nursing dry feed and fermented feed to the pig growth, the result shows, fermented feed is at the colon of pig, digestibility in the rectum is all apparently higher than dry feed (Ole H0jberg, Nuria Canibe, Bettina Knudsen, Bent Borg Jensen.Potential Rates of Fermentation in Digesta from the Gastrointestinal Tract of Pigs:Effect of Feeding Fermented Liquid Feed.J.Appl.Envir.Microbiol, 2003, (69): 408-418).Application of fermentation feeds such as Canibe carry out finding in the pig feeding trial, the lactic acid viable count that fermented feed forms in chitling is apparently higher than dry feed, the feeding effect of fermented feed is (N.Canibe better, B.B.Jensen.Fermented and nonfermented liquid feed to growing pigs:Effect on aspects of gastrointestinal ecology and growth performance.J AnimSci, 2003, (81): 2019-2031).Scholten etc. utilize the test of wheat lactic fermentation forage feed wean pig to show, lactic acid production in the Radix Polygalae Crotalarioidis of fermented feed group is apparently higher than fermented feed group not, and the wheat of feeding after the fermentation can improve the resistance against diseases (R.H.Scholten of wean pig, C.M.van der Peet-Schwering, L.A.denHartog, M.Balk, J.W.Schrama, and M.W.Verstegen Fermented wheat in liquid diets:effects on gastrointestinal characteristics in weanling piglets.J.Anim Sci.2002,80:1179-1186).These prove absolutely that all fermented feed has its special advantages with respect to dry feed, and fermented feed is more and more receiving everybody concern now.
The bajiao banana taro has another name called any of several broadleaf plants lotus root, edible canna etc., belongs to the Cannaceae herbaceos perennial, and the cauline leaf of edible canna can be made into the ensiling green feed.The rhizome dry plate chemical composition of bajiao banana taro is: starch 60%, and moisture 17.1%, ash content 2.8%, crude fibre 2.65%, fat 0.257%, protein 3.63% (Zhu Zuohua. the technical research of bajiao banana taro alcohol fuel solid-state fermentation process, Guizhou University, 2007).The research to the bajiao banana taro abroad mainly concentrates on canna starch grain shape and enzymolysis property aspect (Hung PV thereof, Morita N.PHysicochemical properties and enzymatic digestibility of starch from edible canna (Cannaedulis) grown in Vietnam.Carbohydrate Polymers, 2005, (61): 314 1 321).At present, domesticly existing edible canna is made feed be used to raise pigs: the edible canna instead of corn pork pig of feeding is feasible, because the digestible energy of 4 kilograms of edible cannas is equivalent to 1 kg corn; And feed every kilogram of mixed feed weightening finish joint 0.58 yuan of the edible canna daily ration concentrate feed ratio of feeding, reduce feed cost 9.97% (Ma Weijun. the pork pig test of feeding of edible canna concentrate feed ration formulation, raise pigs, 1997,1:4-5).There are some researches show: edible canna energy content height, and good palatability, can be used as energy feed enter growing-finishing pigs diets (Li Zhengchuan. the nutritive value of edible canna and the effect research of raising pigs, Sichuan animal and veterinary, 1999,5 (26): 25).And domestic research to edible canna more focuses mostly in banana lily starch.Banana lily starch is had many uses, in industry such as lightization, food, pharmacy application is arranged all, as produce citric acid, glucose, alcohol, monosodium glutamate etc., also be applied to process (Fang Qin such as sizing, papermaking, system adhesive in addition, Yao Kexun. the development and utilization of bajiao banana taro resource, China's wild plant resource, 2000,19 (5): 26).Edible canna remaining contained crude fibre, coarse ash equal size of burnt taro slag after extracting starch remains very high, has certain value.
Chinese patent CN101531985 discloses the high-efficiency ferment that a kind of bean pulp fermentation is used, and efficient soybean meal leaven is mixed by bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae and candida utili.Dregs of beans and corn are pulverized, and actication of culture mixes dregs of beans and the zymotic fluid that crushes with mixer,, ferments fermentation back processing: oven dry-pulverizing-check-packing-finished product with the bean pulp fermentation pack that mixes.
Summary of the invention
The object of the present invention is to provide a kind of microbiological treatment edible canna slag to prepare the method for feed.
Described microorganism adopts 5 kinds of microorganism fodder additives that used by Ministry of Agriculture's approval to form, i.e. bacillus subtilis, lactobacillus lactis, Lactobacillus casei, candida utili and saccharomyces cerevisiae.
Concrete operations step of the present invention is as follows:
1) preparation microorganism formulation
Use bacillus subtilis, lactobacillus acidophilus, lactobacillus lactis, candida utili and 5 kinds of microorganism fodder additives of saccharomyces cerevisiae, by percentage to the quality wherein: bacillus subtilis 58%～64%, lactobacillus acidophilus 8%～15%, lactobacillus lactis 5%～11%, candida utili 1%～13%, saccharomyces cerevisiae 10%～16%;
2) preparation feed
With the edible canna ground-slag broken after, add the microorganism formulation that step 1) makes, sealing, fermentation, feed product.
In step 2) in, the water content of described edible canna slag can be 50%～60% by mass percentage, and described pulverizing can be crushed to 38～42 order powder; The addition of described microorganism formulation can be 0.5%～1.5% by mass percentage; The temperature of described fermentation can be 20～28 ℃, and the time of fermentation can be 1～3 day.
The present invention utilizes complex microorganism preparations processing edible canna slag to prepare animal feed, employing is carried out fermentation process to the edible canna slag, for fermented feed is opened up a new source, alleviate China's feed resource problem of shortage to a certain extent, simultaneously resource is reasonably fully utilized, " turn waste into wealth ", meet the development strategy of China's recycling economy, have great social significance.
The present invention can improve the development of exploitation level of feed resource, increases the supply of feedstuff, reduces the grain consumption, can alleviate the feed resource shortage to a certain extent.
Description of drawings
Fig. 1 be in the embodiment of the invention 1～4 EM content to the influence of crude protein content of feed.In Fig. 1, abscissa is EM content %, and ordinate is crude protein content %.
Fig. 2 be in the embodiment of the invention 1～4 EM content to the influence of feed crude fiber content.In Fig. 2, abscissa is EM content %, and ordinate is crude protein content %.
Fig. 3 be in the embodiment of the invention 1～4 EM content to the influence of feed crude fat and coarse ash content.In Fig. 3, abscissa is EM content %, and ordinate is crude fat, coarse ash content %; A is a crude fat; B is a coarse ash.
Fig. 4 be in the embodiment of the invention 1～4 EM content to the influence of feed NFE content.In Fig. 4, abscissa is EM content %, and ordinate is NFE content %.
Fig. 5 divides the influence of content to crude protein content of feed for any of several broadleaf plants space pulp water in the embodiment of the invention 1,5,6.In Fig. 5, abscissa is moisture %, and ordinate is crude protein content %.
Fig. 6 divides the influence of content to feed crude fat, coarse ash content for any of several broadleaf plants space pulp water in the embodiment of the invention 1,5,6.In Fig. 6, abscissa is moisture %, and ordinate is crude fat, coarse ash content %; A is a crude fat; B is a coarse ash.
Fig. 7 divides the influence of content to the feed crude fiber content for any of several broadleaf plants space pulp water in the embodiment of the invention 1,5,6.In Fig. 7, abscissa is moisture %, and ordinate is crude fiber content %.
Fig. 8 divides the influence of content to feed NFE content for any of several broadleaf plants space pulp water in the embodiment of the invention 1,5,6.In Fig. 8, abscissa is moisture %, and ordinate is NFE content %.
Fig. 9 is for adding molasses and not adding the influence of molasses to crude protein content of feed in the embodiment of the invention 1,7.In Fig. 9, abscissa is that 0-adds molasses, and 1-does not add molasses, and ordinate is crude protein content %.
Figure 10 is for adding molasses in the embodiment of the invention 1,7 and not adding molasses to the crude fat of feed, the influence of crude fiber content.In Figure 10, abscissa is that 0-adds molasses, and 1-does not add molasses, and ordinate is crude fat, coarse ash content %; A is a crude fat; B is a coarse ash.
Figure 11 is for adding molasses and not adding the influence of molasses to the crude fiber content of feed in the embodiment of the invention 1,7.In Figure 11, abscissa is that 0-adds molasses, and 1-does not add molasses, and ordinate is fiber content %.
Figure 12 is for adding molasses and not adding the influence of molasses to feed NFE content in the embodiment of the invention 1,7.In Figure 12, abscissa is that 0-adds molasses, and 1-does not add molasses, and ordinate is NFE content %.
Figure 13 is the influence of fate to crude protein content of feed of fermenting in the embodiment of the invention 1,8,9.In Figure 13, abscissa is the fermentation fate, and ordinate is crude protein content %.
Figure 14 is the influence of fate to feed crude fat, coarse ash content of fermenting in the embodiment of the invention 1,8,9.In Figure 14, abscissa is the fermentation fate, and ordinate is crude fat, coarse ash content %; A is a crude fat; B is a coarse ash.
Figure 15 is the influence of fate to the feed crude fiber content of fermenting in the embodiment of the invention 1,8,9.In Figure 15, abscissa is the fermentation fate, and ordinate is crude fiber content %.
Figure 16 is the influence of fate to feed NFE content of fermenting in the embodiment of the invention 1,8,9.In Figure 16, abscissa is the fermentation fate, and ordinate is NFE content %.
The specific embodiment
Following examples will the present invention is further illustrated in conjunction with the accompanying drawings, so that the invention provides foundation for understanding better.
The microorganism formulation (EM) that the present invention adopts is provided by chemical institute of Xiamen University bio-engineering research, the edible canna slag that the edible canna that the edible canna slag is provided by Quanzhou Green's bio tech ltd is left after extracting starch, and the oven dry back is standby.
Measure the pH value with leaching liquor and pH meter method.With the fermented feed of oven dry with Coomassie brilliant blue method, acid-hydrolysis method, soda acid disappear cooking method, high temperature sintering method and measure the content of crude protein, crude fat, crude fibre, coarse ash and the NFE of feed by calculating respectively.Concrete operations are: the water content that takes by weighing Sq is 56% edible canna slag, and behind powder essence 38～42 orders, the adding consumption is 1% microorganism formulation, and then adds suitable molasses, ferments 2 days at 25 ℃ of condition lower seals in the thick plastic bag of packing into after stirring.Result such as Fig. 1～16.
Embodiment 6. is as the fermentation condition of the preparation feed as described in the embodiment 1, investigates the edible canna pulp water and divides content not to the influence of each composition of fermented feed.Its difference is that it is 60% that the edible canna pulp water divides content.Result such as Fig. 5～8.
Embodiment 7. is as the fermentation condition of the preparation feed as described in the embodiment 1, investigates to add molasses and do not add the influence of molasses to each composition of fermented feed.Its difference is not add molasses.Result such as Fig. 9～12.
Embodiment 8. investigates the influence of fermentation fate to each composition of fermented feed as the fermentation condition of the preparation feed as described in the embodiment 1.Its difference fate that is to ferment is 1 day.Result such as Figure 13～16.
Embodiment 9. investigates the influence of fermentation fate to each composition of fermented feed as the fermentation condition of the preparation feed as described in the embodiment 1.Its difference fate that is to ferment is 3 days.Result such as Figure 13～16.
Embodiment 11. investigates the influence of temperature to each composition of fermented feed as the fermentation condition of the preparation feed as described in the embodiment 1.Its difference is that temperature is 20 ℃.
Embodiment 12. investigates the influence of temperature to each composition of fermented feed as the fermentation condition of the preparation feed as described in the embodiment 1.Its difference is that temperature is 28 ℃.
Each constituent content in the table 1 edible canna slag
Table 2EM consumption is to the influence of feed fermentation quality
Table 3 edible canna pulp water divides the influence of content to the feed fermentation quality
Table 4 adds molasses and does not add molasses to the feed fermentation quality influence
Table 5 fermentation fate is to the influence of fermentation quality
Claims (5)
1. microbiological treatment edible canna slag prepares the method for feed, it is characterized in that its concrete operations step is as follows:
1) preparation microorganism formulation
Use bacillus subtilis, lactobacillus acidophilus, lactobacillus lactis, candida utili and 5 kinds of microorganism fodder additives of saccharomyces cerevisiae, by percentage to the quality wherein: bacillus subtilis 58%～64%, lactobacillus acidophilus 8%～15%, lactobacillus lactis 5%～11%, candida utili 1%～13%, saccharomyces cerevisiae 10%～16%;
2) preparation feed
With the edible canna ground-slag broken after, add the microorganism formulation that step 1) makes, sealing, fermentation, feed product.
2. microbiological treatment edible canna slag as claimed in claim 1 prepares the method for feed, it is characterized in that in step 2) in, the water content of described edible canna slag is 50%～60% by mass percentage.
3. microbiological treatment edible canna slag as claimed in claim 1 prepares the method for feed, it is characterized in that in step 2) in, described pulverizing is to be crushed to 38～42 order powder.
4. microbiological treatment edible canna slag as claimed in claim 1 prepares the method for feed, it is characterized in that in step 2) in, the addition of described microorganism formulation is 0.5%～1.5% by mass percentage.
5. microbiological treatment edible canna slag as claimed in claim 1 prepares the method for feed, it is characterized in that in step 2) in, the temperature of described fermentation is 20～28 ℃, the time of fermentation is 1～3 day.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011911A (en) * | 2012-12-14 | 2013-04-03 | 厦门大学 | Method for processing edible canna residue to prepare organic fertilizer by microorganism |
| CN104855702A (en) * | 2015-04-16 | 2015-08-26 | 南宁学院 | Method for preparing cattle feed from siraitia grosvenorii residues |
| CN107333968A (en) * | 2017-08-18 | 2017-11-10 | 广西田阳煦日农业科技发展有限公司 | A kind of non-irrigated lotus root feed addictive |
| CN107821752A (en) * | 2017-11-16 | 2018-03-23 | 广西壮族自治区畜牧研究所 | It is a kind of using purple napier grass, clover and kudzu as ewe feed of major ingredient and preparation method thereof |
| CN108185138A (en) * | 2018-01-09 | 2018-06-22 | 南阳市固德威机械装备有限公司 | A kind of banana dasheen feed processing technology |
| CN108477386A (en) * | 2018-03-06 | 2018-09-04 | 广西田阳煦日农业科技发展有限公司 | A kind of drought lotus root organic feed and preparation method thereof |
| CN109007331A (en) * | 2018-09-10 | 2018-12-18 | 南宁市农业科学研究所 | A kind of banana dasheen feed making method |
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| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101455282A (en) * | 2009-01-09 | 2009-06-17 | 山东六和集团有限公司 | Novel green meat-chicken complete feedstuff and preparation technique thereof |
| CN101531985A (en) * | 2009-04-14 | 2009-09-16 | 厦门六维生物科技有限公司 | High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment |
| CN101983578A (en) * | 2010-11-25 | 2011-03-09 | 杨绍林 | Biochemical feed and preparation method thereof |
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| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101455282A (en) * | 2009-01-09 | 2009-06-17 | 山东六和集团有限公司 | Novel green meat-chicken complete feedstuff and preparation technique thereof |
| CN101531985A (en) * | 2009-04-14 | 2009-09-16 | 厦门六维生物科技有限公司 | High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment |
| CN101983578A (en) * | 2010-11-25 | 2011-03-09 | 杨绍林 | Biochemical feed and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011911A (en) * | 2012-12-14 | 2013-04-03 | 厦门大学 | Method for processing edible canna residue to prepare organic fertilizer by microorganism |
| CN104855702A (en) * | 2015-04-16 | 2015-08-26 | 南宁学院 | Method for preparing cattle feed from siraitia grosvenorii residues |
| CN107333968A (en) * | 2017-08-18 | 2017-11-10 | 广西田阳煦日农业科技发展有限公司 | A kind of non-irrigated lotus root feed addictive |
| CN107821752A (en) * | 2017-11-16 | 2018-03-23 | 广西壮族自治区畜牧研究所 | It is a kind of using purple napier grass, clover and kudzu as ewe feed of major ingredient and preparation method thereof |
| CN108185138A (en) * | 2018-01-09 | 2018-06-22 | 南阳市固德威机械装备有限公司 | A kind of banana dasheen feed processing technology |
| CN108477386A (en) * | 2018-03-06 | 2018-09-04 | 广西田阳煦日农业科技发展有限公司 | A kind of drought lotus root organic feed and preparation method thereof |
| CN109007331A (en) * | 2018-09-10 | 2018-12-18 | 南宁市农业科学研究所 | A kind of banana dasheen feed making method |
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