CN101066090B - Aweto activated reinforced immunological feed additive and its production process - Google Patents
Aweto activated reinforced immunological feed additive and its production process Download PDFInfo
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- CN101066090B CN101066090B CN2007100229237A CN200710022923A CN101066090B CN 101066090 B CN101066090 B CN 101066090B CN 2007100229237 A CN2007100229237 A CN 2007100229237A CN 200710022923 A CN200710022923 A CN 200710022923A CN 101066090 B CN101066090 B CN 101066090B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses one kind of aweto activated and reinforced immunological feed additive and its production process. The materials include corn, wheat bran, rice bran, soybean dregs, inorganic salt, liquid aweto seed and water in the weight ratio of 50-60 to 20-30 to 5-15 to 8-12 to 0.1-0.15 to 8-20 to 40-60; and the production process includes the steps of crushing materials, mixing and opened solid fermentation. The aweto activated and reinforced immunological feed additive contains coarse polysaccharide up to 25.31 % and comprehensive nutritive components, and can activate and reinforce animal's immune system, raise feed utilizing rate, promote animal's growth and raise meat quality. The present invention is favorable to producing green food.
Description
The present invention relates to the feed in the farming, animal husbandry and fishery field, is a kind of feed addictive and production method thereof, particularly a kind of aweto activated reinforced immunological feed additive and production method thereof.
Existing practice and modern pharmacological research all confirm, contain Cordyceps sinensis polysaccharide, cordycepin, protein, superoxide dismutase etc. in northern Chinese caterpillar fungus and the Cordyceps sinensis, can strengthen the immunity of human body, can be used as a kind of reinforced immunological feed additive.Existing mainly is as immunological feed additive northern Chinese caterpillar fungus culture as immunological feed additive.Main with the raising that stimulates various shrimps body immune functions; Like publication number is the one Chinese patent application of CN1448066A a kind of form that comes to this, and it has proposed a kind of immunologic facilitation feed addictive and production method thereof, adopts single northern cordyceps species; With the silkworm and mulberry pupa that steams or boiled; Starch based material corn, wheat bran, rice chaff, granulous wheat, rice etc. are as cultivating batching, sterilization, aseptic condition inoculation down; Solid-state cultivation is 20～30 days under the gnotobasis condition, must contain the northern Chinese caterpillar fungus culture of Cordyceps sinensis polysaccharide 5～20%.Because single northern cordyceps species that adopts and this cultivation batching kind and proportioning make working condition harshly can not carry out open cultivation, incubation time is long, production efficiency is low, the active ingredient kind is few, content is low, a little less than the effect of Product Activation reinforced immunological, narrow application range.
The object of the invention will provide a kind of thick polysaccharide component content high exactly; Be suitable for industrialized production can activated reinforced animal immune system; Improve the immunologic function of animal, thereby improve the efficiency of feed utilization of animal, strengthen the aweto activated reinforced immunological feed additive of assimilation and make this feed addictive production simple; Speed is fast, efficient is high, the measured production method of matter.
The objective of the invention is to realize like this: a kind of aweto activated reinforced immunological feed additive is characterized in that: its raw material comprises liquid spawn, the water of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture; The weight ratio of raw material is: corn: wheat bran: rice chaff: dregs of beans: inorganic salts: the liquid spawn of northern cordyceps species and aweto strain Mixed culture: water=50～60: 20～30: 5～15: 8～12: 0.1～0.15: 8～20: 40～60; Said liquid spawn by northern cordyceps species and aweto strain Mixed culture is meant: become the level liquid bacterial classification by northern cordyceps species and aweto strain by 1: 1 inoculated and cultured; With the level liquid bacterial classification again enlarged culture become second-class liquid isolate, with second-class liquid isolate further three grades of liquid spawns of enlarged culture again; Said inorganic salts are potassium dihydrogen phosphate and magnesium sulfate, and potassium dihydrogen phosphate accounts for 0.07～0.1% of culture medium dry weight, and magnesium sulfate accounts for 0.03～0.05% of culture medium dry weight, and it is to be produced by following method:
A, corn, rice chaff, bean cake powder are broken into 5～10 order meal; Mixing mutually with wheat bran, inorganic salts, water becomes solid medium loose, dampness again, and the liquid-spawn inoculation of northern cordyceps species and aweto strain Mixed culture is become the composite fermentation body to solid medium; The liquid spawn of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture, its mixed weight ratio of water are: 50～60: 20～30: 5～15: 8～12: 0.1～0.15: 8～20: 40～60;
B, with composite fermentation body stand be put on indoor ground or the portable plate or basket, bucket in, the stand puts thickness 3～5cm, ferment;
C, 18 ℃～28 ℃ temperature of maintenance 3～5 days get surperficial inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis that turn white, and are this feed addictive after the compound culture drying of gained.
This aweto activated reinforced immunological feed additive is mixed with vitamin in its solid medium, and vitamin is the independent or vitamin B compound of vitamin(e) B group.
Said aweto activated reinforced immunological feed additive, rice chaff can replace with corncob in the raw material, and dregs of beans can replace with bean powder.
This aweto activated reinforced immunological feed additive in the raw material, is mixed with nucleotides or mixed nucleotides in the solid medium.
The production method of this aweto activated reinforced immunological feed additive is:
A, corn, rice chaff, bean cake powder are broken into 5～10 order meal; Mixing mutually with wheat bran, inorganic salts, water becomes solid medium loose, dampness again, and the liquid-spawn inoculation of northern cordyceps species and aweto strain Mixed culture is become the composite fermentation body to solid medium; The liquid spawn of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture, its mixed weight ratio of water are: 50～60: 20～30: 5～15: 8～12: 0.1-0.15: 8～20: 40～60;
B, with composite fermentation body stand be put on indoor ground or the portable plate or basket, bucket in, thickness 3～5cm is put at the stand, ferments;
C, 18 ℃～28 ℃ temperature of maintenance 3～5 days get surperficial inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis that turn white, and are this feed addictive after the compound culture drying of gained.
The production method of this aweto activated reinforced immunological feed additive, the fermentation that its composite fermentation body carries out is solid-state open type fermented, fermentation time 3～5 days.Open type fermented is that the liquid spawn of northern cordyceps species and aweto strain Mixed culture is inoculated into the composite fermentation body that forms on the solid medium in conventional state and natural environment in conventional state and environment; Need not be at the aseptic condition bottom fermentation, long-time cleaning-less bacteria infection can normally ferment.Cordyceps species or aweto strain carry out solid-state open type fermented then very fast pollution microbes separately, and cultivation can't be carried out.
The present invention has the following advantages: 1, thick polysaccharide component content is up to 25.31%, and multiple components such as cordycepin, protein, superoxide dismutase are complete and content is high; 2, immune system that can activated reinforced animal improves the immunocompetence of animal, thereby improves the efficiency of feed utilization of animal, strengthens assimilation and makes growth of animal fast, and feedstuff-meat ratio was brought up to 2.87: 1 (same basal feed) by 3.26: 1; 3, owing to adopt composite bacteria and this cultivation batching kind of the present invention and proportioning to make solid-state open incubation can resist microbial contamination and growth normally and efficiently by sterilization; Require low to working condition and production environment; Open cultivating and producing is simple, and cost is low; 4, incubation time is short only must 3～5 days shortens 5～7 times of times than alternate manner, and production efficiency is high, and speed is fast, is easy to the big production of heavy industrialization, and quality is good; 5, the effect of Product Activation reinforced immunological is strong; For multiple animals such as pig, ox, sheep, chicken, duck fish, shrimps good effect is arranged all; Applied widely; Do not adding under any antibiotic situation also byer force, can remove that antibiotic interpolation makes animal product can really become pollution-free food in the animal feed from than added with antibiotic control animals resistance against diseases.
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1:
Take by weighing corn 60kg, wheat bran 20kg, rice chaff 10kg, dregs of beans 10kg, water 45kg; Potassium dihydrogen phosphate 0.08kg, magnesium sulfate 0.04kg; 70 of B B-complexes are broken into 5～10 order meal with corn, rice chaff, bean cake powder, and mixing mutually with wheat bran, water becomes solid medium loose, dampness again; The second-class liquid isolate 15kg of northern cordyceps species and aweto strain Mixed culture is inoculated into becomes the composite fermentation body on the solid medium; Composite fermentation body stand is put on the portable plate, and portable plate can move to indoor and outdoor in order to ventilating, dispel the heat, heating, and spreads out summer to put thickness 3cm (spring and autumn sky 4cm, winter 5cm) and carry out open type fermented; Kept 18 ℃～28 ℃ temperature 3 days; White hypha is covered with whole charge level, gets surperficial inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis that turn white, and is this feed addictive after the compound culture drying of gained.The culture that according to said method obtains, wherein thick polyoses content accounts for about 25.31% of its dry weight, and all the other are cordycepin, cordycepic acid, protein, superoxide dismutase, moisture etc.
Embodiment 2:
Take by weighing corn 50gk, wheat bran 30kg, corncob 12kg, bean powder 8kg, water 50kg; Potassium dihydrogen phosphate 0.1kg; Magnesium sulfate 0.05kg, vitamin B compound is a small amount of, with corn, corncob, be ground into 5～10 order meal; Mix mutually with wheat bran, bean powder, water, potassium dihydrogen phosphate, magnesium sulfate, vitamin B compound again and become solid medium loose, dampness; Three grades of liquid of northern cordyceps species and aweto strain Mixed culture are stopped bacterial classification 14kg be inoculated into and become the composite fermentation body on the solid medium, composite fermentation body stand is put on the portable plate, put thickness 3cm (the 5-10 month at the stand; 11-3 month 5cm), carry out open type fermented; Kept 20 ℃ of left and right sides temperature 4 days, white hypha is covered with whole charge level, inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis, and the compound culture of gained is this feed addictive.
Embodiment 3:
Take by weighing corn 60kg, wheat bran 20kg, rice chaff 10kg, dregs of beans 10kg, water 47kg; Potassium dihydrogen phosphate 0.08kg, magnesium sulfate 0.04kg, mixed nucleotides are a small amount of; Corn, rice chaff, bean cake powder are broken into 5～10 order meal; Mix mutually with wheat bran, water again and become solid medium loose, dampness; Three grades of liquid spawn 16kg of northern cordyceps species and aweto strain Mixed culture are added in mixed nucleotides that concentration in the liquid spawn accounts for 100ppm stir to be inoculated into then and become the composite fermentation body on the solid medium, the composite fermentation body is spread out on the portable plate, portable plate can move to indoor and outdoor in order to ventilating, dispel the heat, heat, lowering the temperature.Thickness 3cm is put at the stand, carries out open type fermented; Kept 22 ℃ of left and right sides temperature 2-3 days, and must send out the compound completely culture of bacterium, be this feed addictive after the compound culture drying of gained.The culture that according to said method obtains, thick polyoses content is high, and all the other are complete high for beneficiating ingredient content, the immune system of activated reinforced animal, the ability of the immunity function of raising animal is stronger.
Is the level liquid bacterial classification by northern cordyceps species and aweto strain by the liquid spawn of cultivating on the fluid nutrient medium that is inoculated into by the universal method general formulation at 1: 1; 1000 milliliters of liquid culture mediums are generally put northern cordyceps species and each 1 gram of aweto strain, process the level liquid bacterial classification; With the level liquid bacterial classification is that bacterial classification enlarges that to be inoculated into the bacterium liquid of cultivating on the fluid nutrient medium by the universal method general formulation be second-class liquid isolate; Is three grades of liquid spawns with second-class liquid isolate for further being inoculated into the bacterium liquid of cultivating on the fluid nutrient medium by the universal method general formulation.
The general formulation of cultivating one, two, three liquid spawn is: 1% bean powder, 2% sugar, 3% corn flour, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.1% peptone, vitamin B compound are a small amount of.
Be added in the cultivating system concentration when cultivating one, two, three liquid spawn and at 60-200ppm nucleotides or mixed nucleotides incubation time shortened, spawn activity improves.
The feed that adds this feed addictive account for feed dry weight 3--7% is through, resistance against diseases strong (can remove in animal feed antibiotic interpolation) fast to tests such as pig, ox, sheep, chicken, fish, shrimp growth, meat quality height, make animal product can really become pollution-free food.
Claims (7)
1. aweto activated reinforced immunological feed additive, it is characterized in that: its raw material comprises liquid spawn, the water of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture; The weight ratio of raw material is: corn: wheat bran: rice chaff: dregs of beans: inorganic salts: the liquid spawn of northern cordyceps species and aweto strain Mixed culture: water=50～60: 20～30: 5～15: 8～12: 0.1～0.15: 8～20: 40～60; Said liquid spawn by northern cordyceps species and aweto strain Mixed culture is meant: become the level liquid bacterial classification by northern cordyceps species and aweto strain by 1: 1 inoculated and cultured; With the level liquid bacterial classification again enlarged culture become second-class liquid isolate, with second-class liquid isolate further three grades of liquid spawns of enlarged culture again; Said inorganic salts are potassium dihydrogen phosphate and magnesium sulfate, and potassium dihydrogen phosphate accounts for 0.07～0.1% of culture medium dry weight, and magnesium sulfate accounts for 0.03～0.05% of culture medium dry weight, and it is to be produced by following method:
A, corn, rice chaff, bean cake powder are broken into 5～10 order meal; Mixing mutually with wheat bran, inorganic salts, water becomes solid medium loose, dampness again, and the liquid-spawn inoculation of northern cordyceps species and aweto strain Mixed culture is become the composite fermentation body to solid medium; The liquid spawn of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture, its mixed weight ratio of water are: 50～60: 20～30: 5～15: 8～12: 0.1～0.15: 8～20: 40～60;
B, with composite fermentation body stand be put on indoor ground or the portable plate or basket, bucket in, the stand puts thickness 3～5cm, ferment;
C, 18 ℃～28 ℃ temperature of maintenance 3～5 days get surperficial inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis that turn white, and are this feed addictive after the compound culture drying of gained.
2. according to the said aweto activated reinforced immunological feed additive of claim 1, it is characterized in that: be mixed with vitamin in the solid medium.
3. according to the said aweto activated reinforced immunological feed additive of claim 2, it is characterized in that: the vitamin that is mixed with in the solid medium is vitamin B compound.
4. according to the said aweto activated reinforced immunological feed additive of claim 1, it is characterized in that: rice chaff can replace with corncob, and dregs of beans can replace with bean powder.
5. according to claim 1 or 2 or 3 or 4 said aweto activated reinforced immunological feed additives, it is characterized in that: be mixed with nucleotides or mixed nucleotides in the solid medium.
6. according to the production method of claim 1 aweto activated reinforced immunological feed additive, it is characterized in that:
A, corn, rice chaff, bean cake powder are broken into 5～10 order meal; Mixing mutually with wheat bran, inorganic salts, water becomes solid medium loose, dampness again, and the liquid-spawn inoculation of northern cordyceps species and aweto strain Mixed culture is become the composite fermentation body to solid medium; The liquid spawn of corn, wheat bran, rice chaff, dregs of beans, inorganic salts, northern cordyceps species and aweto strain Mixed culture, its mixed weight ratio of water are: 50～60: 20～30: 5～15: 8～12: 0.1-0.15: 8～20: 40～60;
B, with composite fermentation body stand be put on indoor ground or the portable plate or basket, bucket in, thickness 3～5cm is put at the stand, ferments;
C, 18 ℃～28 ℃ temperature of maintenance 3～5 days get surperficial inner complete white northern Chinese caterpillar fungus and the compound culture of Cordyceps sinensis that turn white, and are this feed addictive after the compound culture drying of gained.
7. according to the production method of the said aweto activated reinforced immunological feed additive of claim 6, it is characterized in that: the fermentation that the composite fermentation body carries out is solid-state open type fermented, fermentation time 3～5 days.
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